Miletić, D., Karličić, V., Lević, S., Nedović, V., Sknepnek, A., Jovičić-Petrović, J.,& Raičević, V.. (2023). Novi tehnološki postupak dobijanja biofortifikatora na bazi Trichoderma spp. obogaćene selenom za primenu u održivoj poljoprivredi. .
https://hdl.handle.net/21.15107/rcub_agrospace_6495

Miletić D, Karličić V, Lević S, Nedović V, Sknepnek A, Jovičić-Petrović J, Raičević V. Novi tehnološki postupak dobijanja biofortifikatora na bazi Trichoderma spp. obogaćene selenom za primenu u održivoj poljoprivredi. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6495 .

Miletić, Dunja, Karličić, Vera, Lević, Steva, Nedović, Viktor, Sknepnek, Aleksandra, Jovičić-Petrović, Jelena, Raičević, Vera, "Novi tehnološki postupak dobijanja biofortifikatora na bazi Trichoderma spp. obogaćene selenom za primenu u održivoj poljoprivredi" (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6495 .

TY  - CONF
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Karličič, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Golubović Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6604
AB  - Organochlorinated pesticides (OCPs), such as DDT are characterized as highly persistent and mobile in environment, with high bioaccumulation capacity. The use of those chemicals has been banned in the United States and Europe for decades (Tsai, 2014). Despite this, OCPs-affected ecosystems are detected in several countries, even in the XXI century. Malusa et al. (2020) reported the presence of DDT in more than 80% of soil samples originating from organic farms in Poland. The objective was to determine the impact of microbial consortium on DDT biodegradation in soil. BacFung microbial consortium consisting of Bacillus sp., Pseudomonas sp., Azotobacter sp. and Trichoderma sp. was used for soil inoculation. Soil conditions were improved by grass sowing. The research was conducted at a playground in Tivat (Montenegro), and the size of the total examined area was 3,000 m2 divided into 13 sub-units. The concentration of DDT and its metabolites (DDE - dichlorodiphenyldichloroethylene and DDD - dichlorodiphenyldichloroethane) was measured by GC/MS technique. The initial DDT concentration in soil sub-units varied from 0.005 to 20.5 mg/kg. The sum of DDT+DDD+DDE (ΣDDT ) concentrations varied from 0.024 to 22.7 mg/kg (average concentration was 1.69 mg/kg). After the application of BacFung microbial consortium, the decline of DDT amount in soil sub-units was observed (from 0.005 to 0.057 mg/kg); ΣDDT was 0.020 to 0.2 mg/kg (average
value 0.055 mg/kg). The accumulation of DDD and DDE, as initial degradation products of DDT, was observed. These results showed that microbial consortium BacFung can be used in removal of DDT from polluted environment.
C3  - 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes
T1  - Microbial consortium in in situ remediation of DDT residues-affected soil
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6604
ER  - 

@conference{
author = "Kljujev, Igor and Lalević, Blažo and Karličič, Vera and Jovičić-Petrović, Jelena and Golubović Ćurguz, Vesna and Raičević, Vera",
year = "2023",
abstract = "Organochlorinated pesticides (OCPs), such as DDT are characterized as highly persistent and mobile in environment, with high bioaccumulation capacity. The use of those chemicals has been banned in the United States and Europe for decades (Tsai, 2014). Despite this, OCPs-affected ecosystems are detected in several countries, even in the XXI century. Malusa et al. (2020) reported the presence of DDT in more than 80% of soil samples originating from organic farms in Poland. The objective was to determine the impact of microbial consortium on DDT biodegradation in soil. BacFung microbial consortium consisting of Bacillus sp., Pseudomonas sp., Azotobacter sp. and Trichoderma sp. was used for soil inoculation. Soil conditions were improved by grass sowing. The research was conducted at a playground in Tivat (Montenegro), and the size of the total examined area was 3,000 m2 divided into 13 sub-units. The concentration of DDT and its metabolites (DDE - dichlorodiphenyldichloroethylene and DDD - dichlorodiphenyldichloroethane) was measured by GC/MS technique. The initial DDT concentration in soil sub-units varied from 0.005 to 20.5 mg/kg. The sum of DDT+DDD+DDE (ΣDDT ) concentrations varied from 0.024 to 22.7 mg/kg (average concentration was 1.69 mg/kg). After the application of BacFung microbial consortium, the decline of DDT amount in soil sub-units was observed (from 0.005 to 0.057 mg/kg); ΣDDT was 0.020 to 0.2 mg/kg (average
value 0.055 mg/kg). The accumulation of DDD and DDE, as initial degradation products of DDT, was observed. These results showed that microbial consortium BacFung can be used in removal of DDT from polluted environment.",
journal = "3rd International Meeting on New Strategies in Bioremediation/Restoration Processes",
title = "Microbial consortium in in situ remediation of DDT residues-affected soil",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6604"
}

Kljujev, I., Lalević, B., Karličič, V., Jovičić-Petrović, J., Golubović Ćurguz, V.,& Raičević, V.. (2023). Microbial consortium in in situ remediation of DDT residues-affected soil. in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes, 154.
https://hdl.handle.net/21.15107/rcub_agrospace_6604

Kljujev I, Lalević B, Karličič V, Jovičić-Petrović J, Golubović Ćurguz V, Raičević V. Microbial consortium in in situ remediation of DDT residues-affected soil. in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes. 2023;:154.
https://hdl.handle.net/21.15107/rcub_agrospace_6604 .

Kljujev, Igor, Lalević, Blažo, Karličič, Vera, Jovičić-Petrović, Jelena, Golubović Ćurguz, Vesna, Raičević, Vera, "Microbial consortium in in situ remediation of DDT residues-affected soil" in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes (2023):154,
https://hdl.handle.net/21.15107/rcub_agrospace_6604 .

TY  - CONF
AU  - Dragojević, Milica
AU  - Đokić, Lidija
AU  - Stanković, Nada
AU  - Raičević, Vera
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6605
AB  - Increased soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.
C3  - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE
T1  - Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6605
ER  - 

@conference{
author = "Dragojević, Milica and Đokić, Lidija and Stanković, Nada and Raičević, Vera and Karličič, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "Increased soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6605"
}

Dragojević, M., Đokić, L., Stanković, N., Raičević, V., Karličič, V., Lalević, B.,& Jovičić-Petrović, J.. (2023). Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE.
https://hdl.handle.net/21.15107/rcub_agrospace_6605

Dragojević M, Đokić L, Stanković N, Raičević V, Karličič V, Lalević B, Jovičić-Petrović J. Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6605 .

Dragojević, Milica, Đokić, Lidija, Stanković, Nada, Raičević, Vera, Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6605 .

TY  - JOUR
AU  - Đedović, Suzana
AU  - Stojanova, Monika
AU  - Bojkovski, Jovan
AU  - Kljujev, Igor
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6836
AB  - Microbial degradation, compared with many other degradation processes, is the most important
pathway for the depletion of triazine herbicides in soil. The aim of this study was to determine the
growth potential of Pseudomonas sp. CY in the presence of atrazine and additional carbon (sodium citrate) and nitrogen (ammonium-nitrate) sources. The experiment was performed with five treatments: i) 100 mg/L atrazine (control); ii) One hundred mg/L atrazine + sodium citrate (0.3 %,w/v); iii) One hundred mg/L atrazine + sodium citrate (0.3 %, w/v) + ammonium nitrate (0.6 %, w/v);iv) Atrazine (300 mg/L) + sodium citrate (0.3 %, w/v) and v) Atrazine (500 mg/L) + sodium citrate (0.3 %, w/v). The bacterial count was determined after incubation (7 days at 30°C) using the agar plate method, while atrazine degradation was determined by measuring the optical density at 221 nm.
Pseudomonas sp. CY can partially utilize atrazine as the sole source of carbon and energy. The
highest values of the bacterial count were determined at the highest initial atrazine concentrations; however, bacterial growth was not detected in these treatments. A significant impact of citrate on bacterial growth and atrazine degradation was observed, while the addition of nitrate decreased the atrazine degradation rate. This study confirmed that Pseudomonas sp. CY can be used as a prominent candidate for the remediation of atrazine-affected environments.
PB  - MDPI
T2  - ZEMLJISTE I BILJKA
T1  - Changes in Pseudomonas sp. CY growth in the presence of atrazine
DO  - 10.5937/ZemBilj2302001D
ER  - 

@article{
author = "Đedović, Suzana and Stojanova, Monika and Bojkovski, Jovan and Kljujev, Igor and Karličič, Vera and Lalević, Blažo and Raičević, Vera",
year = "2023",
abstract = "Microbial degradation, compared with many other degradation processes, is the most important
pathway for the depletion of triazine herbicides in soil. The aim of this study was to determine the
growth potential of Pseudomonas sp. CY in the presence of atrazine and additional carbon (sodium citrate) and nitrogen (ammonium-nitrate) sources. The experiment was performed with five treatments: i) 100 mg/L atrazine (control); ii) One hundred mg/L atrazine + sodium citrate (0.3 %,w/v); iii) One hundred mg/L atrazine + sodium citrate (0.3 %, w/v) + ammonium nitrate (0.6 %, w/v);iv) Atrazine (300 mg/L) + sodium citrate (0.3 %, w/v) and v) Atrazine (500 mg/L) + sodium citrate (0.3 %, w/v). The bacterial count was determined after incubation (7 days at 30°C) using the agar plate method, while atrazine degradation was determined by measuring the optical density at 221 nm.
Pseudomonas sp. CY can partially utilize atrazine as the sole source of carbon and energy. The
highest values of the bacterial count were determined at the highest initial atrazine concentrations; however, bacterial growth was not detected in these treatments. A significant impact of citrate on bacterial growth and atrazine degradation was observed, while the addition of nitrate decreased the atrazine degradation rate. This study confirmed that Pseudomonas sp. CY can be used as a prominent candidate for the remediation of atrazine-affected environments.",
publisher = "MDPI",
journal = "ZEMLJISTE I BILJKA",
title = "Changes in Pseudomonas sp. CY growth in the presence of atrazine",
doi = "10.5937/ZemBilj2302001D"
}

Đedović, S., Stojanova, M., Bojkovski, J., Kljujev, I., Karličič, V., Lalević, B.,& Raičević, V.. (2023). Changes in Pseudomonas sp. CY growth in the presence of atrazine. in ZEMLJISTE I BILJKA
MDPI..
https://doi.org/10.5937/ZemBilj2302001D

Đedović S, Stojanova M, Bojkovski J, Kljujev I, Karličič V, Lalević B, Raičević V. Changes in Pseudomonas sp. CY growth in the presence of atrazine. in ZEMLJISTE I BILJKA. 2023;.
doi:10.5937/ZemBilj2302001D .

Đedović, Suzana, Stojanova, Monika, Bojkovski, Jovan, Kljujev, Igor, Karličič, Vera, Lalević, Blažo, Raičević, Vera, "Changes in Pseudomonas sp. CY growth in the presence of atrazine" in ZEMLJISTE I BILJKA (2023),
https://doi.org/10.5937/ZemBilj2302001D . .

TY  - JOUR
AU  - Dragojević, Milica
AU  - Stankovic, Nada
AU  - Djokic, Lidija
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6316
AB  - The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils. © 2023, The Author(s).
T2  - Environmental Microbiome
T2  - Environmental Microbiome
T1  - Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria
IS  - 1
VL  - 18
DO  - 10.1186/s40793-023-00477-x
ER  - 

@article{
author = "Dragojević, Milica and Stankovic, Nada and Djokic, Lidija and Raičević, Vera and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils. © 2023, The Author(s).",
journal = "Environmental Microbiome, Environmental Microbiome",
title = "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria",
number = "1",
volume = "18",
doi = "10.1186/s40793-023-00477-x"
}

Dragojević, M., Stankovic, N., Djokic, L., Raičević, V.,& Jovičić-Petrović, J.. (2023). Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome, 18(1).
https://doi.org/10.1186/s40793-023-00477-x

Dragojević M, Stankovic N, Djokic L, Raičević V, Jovičić-Petrović J. Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome. 2023;18(1).
doi:10.1186/s40793-023-00477-x .

Dragojević, Milica, Stankovic, Nada, Djokic, Lidija, Raičević, Vera, Jovičić-Petrović, Jelena, "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria" in Environmental Microbiome, 18, no. 1 (2023),
https://doi.org/10.1186/s40793-023-00477-x . .

TY  - JOUR
AU  - Todorović, Irena
AU  - Abrouk, Danis
AU  - Kyselková, Martina
AU  - Lavire, Céline
AU  - Rey, Marjolaine
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Moënne-Loccoz, Yvan
AU  - Muller, Daniel
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6337
AB  - Pseudomonas strains IT-194P, IT-215P, IT-P366T and IT-P374T were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366T and IT-194P and clustering (whole genome analysis) next to P. umsongensis DSM16611T, and another species containing strains IT-P374T and IT-215P and clustering next to P. koreensis LMG21318T. Genome analysis confirmed the proposition of novel species, as ANI was below the threshold of 95% and dDDH below 70% for strains IT-P366T (compared with P. umsongensis DSM16611T) and IT-P374T (compared with P. koreensis LMG21318T). Unlike P. umsongensis DSM16611T, strains of P. serbica can grow on D-mannitol, but not on pectin, D-galacturonic acid, L-galactonic acid lactone and α-hydroxybutyric acid. In contrary to P. koreensis LMG21318T, strains of P. serboccidentalis can use sucrose, inosine and α-ketoglutaric acid (but not L-histidine) as carbon sources. Altogether, these results indicate the existence of two novel species for which we propose the names Pseudomonas serbica sp. nov., with the type strain IT-P366T (=CFBP 9060 T = LMG 32732 T = EML 1791 T) and Pseudomonas serboccidentalis sp. nov., with the type strain IT-P374T (=CFBP 9061 T = LMG 32734 T = EML 1792 T). Strains from this study presented a set of phytobeneficial functions modulating plant hormonal balance, plant nutrition and plant protection, suggesting a potential as Plant Growth-Promoting Rhizobacteria (PGPR). © 2023 Elsevier GmbH
T2  - Systematic and Applied Microbiology
T2  - Systematic and Applied Microbiology
T1  - Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas
serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.
IS  - 4
VL  - 46
DO  - 10.1016/j.syapm.2023.126425
ER  - 

@article{
author = "Todorović, Irena and Abrouk, Danis and Kyselková, Martina and Lavire, Céline and Rey, Marjolaine and Raičević, Vera and Jovičić-Petrović, Jelena and Moënne-Loccoz, Yvan and Muller, Daniel",
year = "2023",
abstract = "Pseudomonas strains IT-194P, IT-215P, IT-P366T and IT-P374T were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366T and IT-194P and clustering (whole genome analysis) next to P. umsongensis DSM16611T, and another species containing strains IT-P374T and IT-215P and clustering next to P. koreensis LMG21318T. Genome analysis confirmed the proposition of novel species, as ANI was below the threshold of 95% and dDDH below 70% for strains IT-P366T (compared with P. umsongensis DSM16611T) and IT-P374T (compared with P. koreensis LMG21318T). Unlike P. umsongensis DSM16611T, strains of P. serbica can grow on D-mannitol, but not on pectin, D-galacturonic acid, L-galactonic acid lactone and α-hydroxybutyric acid. In contrary to P. koreensis LMG21318T, strains of P. serboccidentalis can use sucrose, inosine and α-ketoglutaric acid (but not L-histidine) as carbon sources. Altogether, these results indicate the existence of two novel species for which we propose the names Pseudomonas serbica sp. nov., with the type strain IT-P366T (=CFBP 9060 T = LMG 32732 T = EML 1791 T) and Pseudomonas serboccidentalis sp. nov., with the type strain IT-P374T (=CFBP 9061 T = LMG 32734 T = EML 1792 T). Strains from this study presented a set of phytobeneficial functions modulating plant hormonal balance, plant nutrition and plant protection, suggesting a potential as Plant Growth-Promoting Rhizobacteria (PGPR). © 2023 Elsevier GmbH",
journal = "Systematic and Applied Microbiology, Systematic and Applied Microbiology",
title = "Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas
serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.",
number = "4",
volume = "46",
doi = "10.1016/j.syapm.2023.126425"
}

Todorović, I., Abrouk, D., Kyselková, M., Lavire, C., Rey, M., Raičević, V., Jovičić-Petrović, J., Moënne-Loccoz, Y.,& Muller, D.. (2023). Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas
serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.. in Systematic and Applied Microbiology, 46(4).
https://doi.org/10.1016/j.syapm.2023.126425

Todorović I, Abrouk D, Kyselková M, Lavire C, Rey M, Raičević V, Jovičić-Petrović J, Moënne-Loccoz Y, Muller D. Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas
serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.. in Systematic and Applied Microbiology. 2023;46(4).
doi:10.1016/j.syapm.2023.126425 .

Todorović, Irena, Abrouk, Danis, Kyselková, Martina, Lavire, Céline, Rey, Marjolaine, Raičević, Vera, Jovičić-Petrović, Jelena, Moënne-Loccoz, Yvan, Muller, Daniel, "Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas
serbica sp. nov. and Pseudomonas serboccidentalis sp. nov." in Systematic and Applied Microbiology, 46, no. 4 (2023),
https://doi.org/10.1016/j.syapm.2023.126425 . .

TY  - JOUR
AU  - Todorović, Irena
AU  - Yvan, Moenne-Loccoz
AU  - Vera, Raičević
AU  - Jelena, Jovičić-Petrović
AU  - Daniel, Muller
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6529
AB  - Fusarium species are cosmopolitan soil phytopathogens from the division Ascomycota, which produce mycotoxins and cause significant economic losses of crop plants. However, soils suppressive to Fusarium diseases are known to occur, and recent knowledge on microbial diversity in these soils has shed new lights on phytoprotection effects. In this review, we synthesize current knowledge on soils suppressive to Fusarium diseases and the role of their rhizosphere microbiota in phytoprotection. This is an important issue, as disease does not develop significantly in suppressive soils even though pathogenic Fusarium and susceptible host plant are present, and weather conditions are suitable for disease. Soils suppressive to Fusarium diseases are documented in different regions of the world. They contain biocontrol microorganisms, which act by inducing plants’ resistance to the pathogen, competing with or inhibiting the pathogen, or parasitizing the pathogen. In particular, some of the Bacillus, Pseudomonas, Paenibacillus and Streptomyces species are involved in plant protection from Fusarium diseases. Besides specific bacterial populations involved in disease suppression, next-generation sequencing and ecological networks have largely contributed to the understanding of microbial communities in soils suppressive or not to Fusarium diseases, revealing different microbial community patterns and differences for a notable number of taxa, according to the Fusarium pathosystem, the host plant and the origin of the soil. Agricultural practices can significantly influence soil suppressiveness to Fusarium diseases by influencing soil microbiota ecology. Research on microbial modes of action and diversity in suppressive soils should help guide the development of effective farming practices for Fusarium disease management in sustainable agriculture.
PB  - Frontiers
T2  - Frontiers in Plant Science
T1  - Microbial diversity in soils suppressive to Fusarium diseases
VL  - 14
DO  - 10.3389/fpls.2023.1228749
ER  - 

@article{
author = "Todorović, Irena and Yvan, Moenne-Loccoz and Vera, Raičević and Jelena, Jovičić-Petrović and Daniel, Muller",
year = "2023",
abstract = "Fusarium species are cosmopolitan soil phytopathogens from the division Ascomycota, which produce mycotoxins and cause significant economic losses of crop plants. However, soils suppressive to Fusarium diseases are known to occur, and recent knowledge on microbial diversity in these soils has shed new lights on phytoprotection effects. In this review, we synthesize current knowledge on soils suppressive to Fusarium diseases and the role of their rhizosphere microbiota in phytoprotection. This is an important issue, as disease does not develop significantly in suppressive soils even though pathogenic Fusarium and susceptible host plant are present, and weather conditions are suitable for disease. Soils suppressive to Fusarium diseases are documented in different regions of the world. They contain biocontrol microorganisms, which act by inducing plants’ resistance to the pathogen, competing with or inhibiting the pathogen, or parasitizing the pathogen. In particular, some of the Bacillus, Pseudomonas, Paenibacillus and Streptomyces species are involved in plant protection from Fusarium diseases. Besides specific bacterial populations involved in disease suppression, next-generation sequencing and ecological networks have largely contributed to the understanding of microbial communities in soils suppressive or not to Fusarium diseases, revealing different microbial community patterns and differences for a notable number of taxa, according to the Fusarium pathosystem, the host plant and the origin of the soil. Agricultural practices can significantly influence soil suppressiveness to Fusarium diseases by influencing soil microbiota ecology. Research on microbial modes of action and diversity in suppressive soils should help guide the development of effective farming practices for Fusarium disease management in sustainable agriculture.",
publisher = "Frontiers",
journal = "Frontiers in Plant Science",
title = "Microbial diversity in soils suppressive to Fusarium diseases",
volume = "14",
doi = "10.3389/fpls.2023.1228749"
}

Todorović, I., Yvan, M., Vera, R., Jelena, J.,& Daniel, M.. (2023). Microbial diversity in soils suppressive to Fusarium diseases. in Frontiers in Plant Science
Frontiers., 14.
https://doi.org/10.3389/fpls.2023.1228749

Todorović I, Yvan M, Vera R, Jelena J, Daniel M. Microbial diversity in soils suppressive to Fusarium diseases. in Frontiers in Plant Science. 2023;14.
doi:10.3389/fpls.2023.1228749 .

Todorović, Irena, Yvan, Moenne-Loccoz, Vera, Raičević, Jelena, Jovičić-Petrović, Daniel, Muller, "Microbial diversity in soils suppressive to Fusarium diseases" in Frontiers in Plant Science, 14 (2023),
https://doi.org/10.3389/fpls.2023.1228749 . .

TY  - CONF
AU  - Karličič, Vera
AU  - Dragojević, Milica
AU  - Savić, Zagorka
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6606
AB  - Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.
C3  - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE
T1  - Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6606
ER  - 

@conference{
author = "Karličič, Vera and Dragojević, Milica and Savić, Zagorka and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2023",
abstract = "Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6606"
}

Karličič, V., Dragojević, M., Savić, Z., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2023). Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE.
https://hdl.handle.net/21.15107/rcub_agrospace_6606

Karličič V, Dragojević M, Savić Z, Jovičić-Petrović J, Kljujev I, Raičević V. Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

Karličič, Vera, Dragojević, Milica, Savić, Zagorka, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

TY  - CONF
AU  - Karličić, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Raičević, Vera
AU  - Kljujev, Igor
AU  - Gudalović, Tatjana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6511
AB  - One of the major problems in agriculture are Fusarium species which cause fusariosis in wheat,
corn, tomato, melon, watermelon, onion, peas, and beans. Also, Fusarium can synthesize
thermostable mycotoxins which can lead to certain diseases if they were found in food.
Currently, chemical fungicides are mostly used to prevent the occurrence of fusariosis disease,
but the resistance of pathogens to such treatment is being more and more widespread. Soil
microorganisms help in plant defense and growth. The rhizosphere fungi have an important role
in the mutual exchange of nutrients with plants and they can establish specific interactions with
plants. Such rhizosphere fungi are known as plant growth-promoting (PGP). These PGP fungi
affect the better plant growth through the synthesis of certain phytohormones but they also have
the function as biocontrol agents. They can inhibit the growth of phytopathogens through direct
mechanisms of antibiosis, mycoparasitism, and competition.
The goal of this research was an investigation of the antagonistic effect of Trichoderma
harzianum TR1 and Trichoderma citrinoviride 1V on Fusarium oxysporum and Fusarium
graminearum. The antagonistic effect was examined through two tests: Dual culture test (DUAL
test) and the effect of volatile organic compounds (VOCs) of Trichoderma strains on the growth
of Fusarium strains. Also, the cell-wall degrading enzymatic activity of T. citrinoviride and T.
harzianum was examined. The results showed that antagonistic activity of Trichoderma strains
varies from moderate to high. Mycelial growth inhibition by T. citrinoviride was 44% for F.
graminearum and 67% for F. oxysporum. T. harzianum inhibited F. gramminearum growth for
59% and F. oxysporum for 66%. Based on the results, it was concluded that T.harzianum and
T.citrinoviride can be considered biocontrol agents for F. oxysporum and F. graminearum.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - ...
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
T1  - In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6511
ER  - 

@conference{
author = "Karličić, Vera and Jovičić-Petrović, Jelena and Lalević, Blažo and Raičević, Vera and Kljujev, Igor and Gudalović, Tatjana",
year = "2022",
abstract = "One of the major problems in agriculture are Fusarium species which cause fusariosis in wheat,
corn, tomato, melon, watermelon, onion, peas, and beans. Also, Fusarium can synthesize
thermostable mycotoxins which can lead to certain diseases if they were found in food.
Currently, chemical fungicides are mostly used to prevent the occurrence of fusariosis disease,
but the resistance of pathogens to such treatment is being more and more widespread. Soil
microorganisms help in plant defense and growth. The rhizosphere fungi have an important role
in the mutual exchange of nutrients with plants and they can establish specific interactions with
plants. Such rhizosphere fungi are known as plant growth-promoting (PGP). These PGP fungi
affect the better plant growth through the synthesis of certain phytohormones but they also have
the function as biocontrol agents. They can inhibit the growth of phytopathogens through direct
mechanisms of antibiosis, mycoparasitism, and competition.
The goal of this research was an investigation of the antagonistic effect of Trichoderma
harzianum TR1 and Trichoderma citrinoviride 1V on Fusarium oxysporum and Fusarium
graminearum. The antagonistic effect was examined through two tests: Dual culture test (DUAL
test) and the effect of volatile organic compounds (VOCs) of Trichoderma strains on the growth
of Fusarium strains. Also, the cell-wall degrading enzymatic activity of T. citrinoviride and T.
harzianum was examined. The results showed that antagonistic activity of Trichoderma strains
varies from moderate to high. Mycelial growth inhibition by T. citrinoviride was 44% for F.
graminearum and 67% for F. oxysporum. T. harzianum inhibited F. gramminearum growth for
59% and F. oxysporum for 66%. Based on the results, it was concluded that T.harzianum and
T.citrinoviride can be considered biocontrol agents for F. oxysporum and F. graminearum.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, ...",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”",
title = "In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6511"
}

Karličić, V., Jovičić-Petrović, J., Lalević, B., Raičević, V., Kljujev, I.,& Gudalović, T.. (2022). In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6511

Karličić V, Jovičić-Petrović J, Lalević B, Raičević V, Kljujev I, Gudalović T. In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6511 .

Karličić, Vera, Jovičić-Petrović, Jelena, Lalević, Blažo, Raičević, Vera, Kljujev, Igor, Gudalović, Tatjana, "In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6511 .

TY  - CONF
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6603
AB  - Biopriming is a pre-sowing seed inoculation technique based on beneficial microorganisms. The aim of the study was to estimate biopriming potential of microbial consortium on soybean seeds. Obtained results showed a stimulative effect of biopriming resulting in 8% higher germination percentage. Inoculated seeds were characterised as more vigorous according to vigor I, vigor II and electrical conductivity results. Accelerated aging test decreased germination percentage of both treatments to approximately 66±2%. Inoculated seeds were marked as more vigorous according to vigor I index. Biopriming of soybean seeds resulted in an increase of germination parameters that suggest stimulative effects on seed quality.
C3  - 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia
T1  - Biopriming: multiple effects on soybean germination metrics
EP  - 282
SP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6603
ER  - 

@conference{
author = "Karličič, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2022",
abstract = "Biopriming is a pre-sowing seed inoculation technique based on beneficial microorganisms. The aim of the study was to estimate biopriming potential of microbial consortium on soybean seeds. Obtained results showed a stimulative effect of biopriming resulting in 8% higher germination percentage. Inoculated seeds were characterised as more vigorous according to vigor I, vigor II and electrical conductivity results. Accelerated aging test decreased germination percentage of both treatments to approximately 66±2%. Inoculated seeds were marked as more vigorous according to vigor I index. Biopriming of soybean seeds resulted in an increase of germination parameters that suggest stimulative effects on seed quality.",
journal = "57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia",
title = "Biopriming: multiple effects on soybean germination metrics",
pages = "282-278",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6603"
}

Karličič, V., Lalević, B., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2022). Biopriming: multiple effects on soybean germination metrics. in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia, 278-282.
https://hdl.handle.net/21.15107/rcub_agrospace_6603

Karličič V, Lalević B, Jovičić-Petrović J, Kljujev I, Raičević V. Biopriming: multiple effects on soybean germination metrics. in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia. 2022;:278-282.
https://hdl.handle.net/21.15107/rcub_agrospace_6603 .

Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Biopriming: multiple effects on soybean germination metrics" in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia (2022):278-282,
https://hdl.handle.net/21.15107/rcub_agrospace_6603 .

Raičević, V., Karličič, V., Lalević, B., Jovičić-Petrović, J., Kljujev, I.,& Golubović Ćurguz, V.. (2022). Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola. 
University of Belgrade, Faculty of Agriculture, Serbia..
https://hdl.handle.net/21.15107/rcub_agrospace_6723

Raičević V, Karličič V, Lalević B, Jovičić-Petrović J, Kljujev I, Golubović Ćurguz V. Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6723 .

Raičević, Vera, Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, Kljujev, Igor, Golubović Ćurguz, Vesna, "Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola" (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6723 .

TY  - GEN
AU  - Karličič, Vera
AU  - Simić, Aleksandar
AU  - Brajević, Snežana
AU  - Kljujev, Igor
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6724
AB  - Eksploatacija rude, i pored benefita za ljudsko društvo, dovodi do pogoršanja kvaliteta životne sredine. Kao posledica eksploatacije, dolazi do deponovanja otpadnog materijala i stvaranja jalovina sa nepovoljnim fizičkim, hemijskim i biološkim osobinama. Imajući u vidu značaj mikroorganizama u oštećenim ekosistemima, cilj ovog rada bio je ispitivanje mikrobnog diverziteta jalovine; na jednom delu jalovine izvršena je setva semena crvenog vijuka uz dodatak organskog i mineralnog đubriva (CVF), na drugom setva istog semena bez đubrenja (CV), dok je treći deo, bez setve i đubrenja, predstavljao kontrolu (KON). Mikrobni diverzitet ispitan je metodom agarnih ploča, pri čemu je određen ukupan broj bakterija, amonifikatora i gljiva, kao i prisustvo vrsta iz rodova Pseudomonas sp. i Azotobacter sp. Dobijeni rezultati ukazuju na nizak ukupan broj bakterija, amonifikatora i gljiva, dok vrste iz rodova Pseudomonas sp. i Azotobacter sp. nisu detektovane. Slaba zastupljenost mikrobnih populacija je posledica niskog sadržaja hranljivih supstanci i visoke koncentracije teških metala. Đubrenje je uticalo na povećanje mikrobiološke aktivnosti, kao i na promenu odnosa fungalnih i bakterijskih populacija, što ukazuje na oporavak oštećenog ekosistema.
T2  - XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac
T1  - Mikrobni diverzitet kao pokazatelj remedijacije jalovine
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6724
ER  - 

@misc{
author = "Karličič, Vera and Simić, Aleksandar and Brajević, Snežana and Kljujev, Igor and Jovičić-Petrović, Jelena and Raičević, Vera and Lalević, Blažo",
year = "2022",
abstract = "Eksploatacija rude, i pored benefita za ljudsko društvo, dovodi do pogoršanja kvaliteta životne sredine. Kao posledica eksploatacije, dolazi do deponovanja otpadnog materijala i stvaranja jalovina sa nepovoljnim fizičkim, hemijskim i biološkim osobinama. Imajući u vidu značaj mikroorganizama u oštećenim ekosistemima, cilj ovog rada bio je ispitivanje mikrobnog diverziteta jalovine; na jednom delu jalovine izvršena je setva semena crvenog vijuka uz dodatak organskog i mineralnog đubriva (CVF), na drugom setva istog semena bez đubrenja (CV), dok je treći deo, bez setve i đubrenja, predstavljao kontrolu (KON). Mikrobni diverzitet ispitan je metodom agarnih ploča, pri čemu je određen ukupan broj bakterija, amonifikatora i gljiva, kao i prisustvo vrsta iz rodova Pseudomonas sp. i Azotobacter sp. Dobijeni rezultati ukazuju na nizak ukupan broj bakterija, amonifikatora i gljiva, dok vrste iz rodova Pseudomonas sp. i Azotobacter sp. nisu detektovane. Slaba zastupljenost mikrobnih populacija je posledica niskog sadržaja hranljivih supstanci i visoke koncentracije teških metala. Đubrenje je uticalo na povećanje mikrobiološke aktivnosti, kao i na promenu odnosa fungalnih i bakterijskih populacija, što ukazuje na oporavak oštećenog ekosistema.",
journal = "XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac",
title = "Mikrobni diverzitet kao pokazatelj remedijacije jalovine",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6724"
}

Karličič, V., Simić, A., Brajević, S., Kljujev, I., Jovičić-Petrović, J., Raičević, V.,& Lalević, B.. (2022). Mikrobni diverzitet kao pokazatelj remedijacije jalovine. in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac.
https://hdl.handle.net/21.15107/rcub_agrospace_6724

Karličič V, Simić A, Brajević S, Kljujev I, Jovičić-Petrović J, Raičević V, Lalević B. Mikrobni diverzitet kao pokazatelj remedijacije jalovine. in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6724 .

Karličič, Vera, Simić, Aleksandar, Brajević, Snežana, Kljujev, Igor, Jovičić-Petrović, Jelena, Raičević, Vera, Lalević, Blažo, "Mikrobni diverzitet kao pokazatelj remedijacije jalovine" in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6724 .

TY  - JOUR
AU  - Radić, Danka
AU  - Karličić, Vera
AU  - Đorđević, Jelena
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6116
AB  - A large number of soil microorganisms are characterized as plant growth promoting, but there seems to be a lack of comprehensive knowledge regarding plant growth promoting soil yeasts. The aim of the experiment was to analyse the properties of three yeast species: Schwanniomyces occidentalis BK0302D, Cyberlindnera saturnus CK2404I and Candida tropicalis 2TD2912B, important for plant growth (ammonium sulphate transformation, phosphorus, potassium and zinc dissolution), and to evaluate the effect of yeast on the growth of common wheat and white mustard seedlings after seeds’ inoculation. Common wheat and white mustard seeds were inoculated with the selected yeasts. The final measurements showed that the highest amount of nitrate (10.40 μg mL-1 NO3 −) was produced by C. saturnus CK2404I, while S. occidentalis BK0302D solubilized the largest amount of phosphorus (63.70 μg mL-1 P). All three strains are marked as potassium and zinc solubilizers with both acid and alkaline phosphatase activity. This is the first report on S. occidentalis and C. tropicalis ability to solubilize insoluble potassium and zinc, and C. saturnus ability to solubilize insoluble phosphorus, potassium and zinc. Also, C. tropicalis 2TD2912B exhibited high antagonistic activity (66% growth inhibition) toward Botrytis cinerea. In vivo trial was conducted in a low-nutrient substrate, and S. occidentalis BK0302D was found to have the most considerable influence on common wheat biomass production (34% increase). White mustard inoculation with C. saturnus CK2404I resulted in a 4-fold higher biomass production, while S. occidentalis BK0302D induced a 2-fold increase. The presented results confirmed the multi-functional plant growth promoting characteristics of the tested yeasts and their potential for broad application from conventional agriculture on low-nutrient soils to revegetation of disturbed substrates. © 2022, Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University. All rights reserved.
T2  - Zemdirbyste
T2  - Zemdirbyste
T1  - Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard
EP  - 34
IS  - 1
SP  - 27
VL  - 109
DO  - 10.13080/Z-A.2022.109.004
ER  - 

@article{
author = "Radić, Danka and Karličić, Vera and Đorđević, Jelena and Jovičić-Petrović, Jelena and Kljujev, Igor and Lalević, Blažo and Raičević, Vera",
year = "2022",
abstract = "A large number of soil microorganisms are characterized as plant growth promoting, but there seems to be a lack of comprehensive knowledge regarding plant growth promoting soil yeasts. The aim of the experiment was to analyse the properties of three yeast species: Schwanniomyces occidentalis BK0302D, Cyberlindnera saturnus CK2404I and Candida tropicalis 2TD2912B, important for plant growth (ammonium sulphate transformation, phosphorus, potassium and zinc dissolution), and to evaluate the effect of yeast on the growth of common wheat and white mustard seedlings after seeds’ inoculation. Common wheat and white mustard seeds were inoculated with the selected yeasts. The final measurements showed that the highest amount of nitrate (10.40 μg mL-1 NO3 −) was produced by C. saturnus CK2404I, while S. occidentalis BK0302D solubilized the largest amount of phosphorus (63.70 μg mL-1 P). All three strains are marked as potassium and zinc solubilizers with both acid and alkaline phosphatase activity. This is the first report on S. occidentalis and C. tropicalis ability to solubilize insoluble potassium and zinc, and C. saturnus ability to solubilize insoluble phosphorus, potassium and zinc. Also, C. tropicalis 2TD2912B exhibited high antagonistic activity (66% growth inhibition) toward Botrytis cinerea. In vivo trial was conducted in a low-nutrient substrate, and S. occidentalis BK0302D was found to have the most considerable influence on common wheat biomass production (34% increase). White mustard inoculation with C. saturnus CK2404I resulted in a 4-fold higher biomass production, while S. occidentalis BK0302D induced a 2-fold increase. The presented results confirmed the multi-functional plant growth promoting characteristics of the tested yeasts and their potential for broad application from conventional agriculture on low-nutrient soils to revegetation of disturbed substrates. © 2022, Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University. All rights reserved.",
journal = "Zemdirbyste, Zemdirbyste",
title = "Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard",
pages = "34-27",
number = "1",
volume = "109",
doi = "10.13080/Z-A.2022.109.004"
}

Radić, D., Karličić, V., Đorđević, J., Jovičić-Petrović, J., Kljujev, I., Lalević, B.,& Raičević, V.. (2022). Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard. in Zemdirbyste, 109(1), 27-34.
https://doi.org/10.13080/Z-A.2022.109.004

Radić D, Karličić V, Đorđević J, Jovičić-Petrović J, Kljujev I, Lalević B, Raičević V. Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard. in Zemdirbyste. 2022;109(1):27-34.
doi:10.13080/Z-A.2022.109.004 .

Radić, Danka, Karličić, Vera, Đorđević, Jelena, Jovičić-Petrović, Jelena, Kljujev, Igor, Lalević, Blažo, Raičević, Vera, "Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard" in Zemdirbyste, 109, no. 1 (2022):27-34,
https://doi.org/10.13080/Z-A.2022.109.004 . .

TY  - JOUR
AU  - Kerečki, Slavica
AU  - Pećinar, Ilinka
AU  - Karličić, Vera
AU  - Mirković, Nemanja
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6143
AB  - This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
T2  - Journal of Plant Interactions
T2  - Journal of Plant Interactions
T1  - Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming
EP  - 730
IS  - 1
SP  - 719
VL  - 17
DO  - 10.1080/17429145.2022.2091802
ER  - 

@article{
author = "Kerečki, Slavica and Pećinar, Ilinka and Karličić, Vera and Mirković, Nemanja and Kljujev, Igor and Raičević, Vera and Jovičić-Petrović, Jelena",
year = "2022",
abstract = "This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",
journal = "Journal of Plant Interactions, Journal of Plant Interactions",
title = "Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming",
pages = "730-719",
number = "1",
volume = "17",
doi = "10.1080/17429145.2022.2091802"
}

Kerečki, S., Pećinar, I., Karličić, V., Mirković, N., Kljujev, I., Raičević, V.,& Jovičić-Petrović, J.. (2022). Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming. in Journal of Plant Interactions, 17(1), 719-730.
https://doi.org/10.1080/17429145.2022.2091802

Kerečki S, Pećinar I, Karličić V, Mirković N, Kljujev I, Raičević V, Jovičić-Petrović J. Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming. in Journal of Plant Interactions. 2022;17(1):719-730.
doi:10.1080/17429145.2022.2091802 .

Kerečki, Slavica, Pećinar, Ilinka, Karličić, Vera, Mirković, Nemanja, Kljujev, Igor, Raičević, Vera, Jovičić-Petrović, Jelena, "Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming" in Journal of Plant Interactions, 17, no. 1 (2022):719-730,
https://doi.org/10.1080/17429145.2022.2091802 . .

TY  - JOUR
AU  - Mandić, N.
AU  - Lalević, B.
AU  - Raičević, V.
AU  - Radojičić, V.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6159
AB  - In the past several decades, due to the increased population, the planet has faced immense problems with waste management.
The need for recycling and upcycling is increasing. It has become imperative to extract maximum value from discarded
products. There are nearly one billion smokers on the planet. Tobacco butts are toxic, and they pollute the environment. In
this paper, the research objective was the bio-augmentation of waste in the context of composting tobacco and vegetable
waste mixtures, and the determination of nicotine degradation rate depending on the composting conditions. Low-grade
Virginia tobacco leaves that were discarded from further processing were used as base material. The experiment was conducted in triplicate. To each sample, bacteria from the species Brevibacillus were added, and then, samples were composted
under diferent conditions (controlled, semi-controlled and non-controlled). Two more samples were formed as the control
group, one without bacteria and one with only tobacco waste. The research was conducted over the period of one month
with monitoring of the process parameters. The tobacco waste had a baseline nicotine level of 17,363 mg/kg. In all samples
containing nicotine-degrading bacteria, the level of nicotine dropped below 10 mg/kg. There was a direct positive relationship between the rate of nicotine degradation, temperature and moisture content in the samples, as well as environmental
temperature and relative humidity. The content of macro- and micronutrients makes the compost appropriate for organic
fertilization in agricultural production.
PB  - Springer
T2  - International Journal of Environmental Science and Technology
T1  - Impact of composting conditions on the nicotine degradation rate using nicotinophilic bacteria from tobacco waste
VL  - 1
DO  - 10.1007/s13762-022-04405-3
ER  - 

@article{
author = "Mandić, N. and Lalević, B. and Raičević, V. and Radojičić, V.",
year = "2022",
abstract = "In the past several decades, due to the increased population, the planet has faced immense problems with waste management.
The need for recycling and upcycling is increasing. It has become imperative to extract maximum value from discarded
products. There are nearly one billion smokers on the planet. Tobacco butts are toxic, and they pollute the environment. In
this paper, the research objective was the bio-augmentation of waste in the context of composting tobacco and vegetable
waste mixtures, and the determination of nicotine degradation rate depending on the composting conditions. Low-grade
Virginia tobacco leaves that were discarded from further processing were used as base material. The experiment was conducted in triplicate. To each sample, bacteria from the species Brevibacillus were added, and then, samples were composted
under diferent conditions (controlled, semi-controlled and non-controlled). Two more samples were formed as the control
group, one without bacteria and one with only tobacco waste. The research was conducted over the period of one month
with monitoring of the process parameters. The tobacco waste had a baseline nicotine level of 17,363 mg/kg. In all samples
containing nicotine-degrading bacteria, the level of nicotine dropped below 10 mg/kg. There was a direct positive relationship between the rate of nicotine degradation, temperature and moisture content in the samples, as well as environmental
temperature and relative humidity. The content of macro- and micronutrients makes the compost appropriate for organic
fertilization in agricultural production.",
publisher = "Springer",
journal = "International Journal of Environmental Science and Technology",
title = "Impact of composting conditions on the nicotine degradation rate using nicotinophilic bacteria from tobacco waste",
volume = "1",
doi = "10.1007/s13762-022-04405-3"
}

Mandić, N., Lalević, B., Raičević, V.,& Radojičić, V.. (2022). Impact of composting conditions on the nicotine degradation rate using nicotinophilic bacteria from tobacco waste. in International Journal of Environmental Science and Technology
Springer., 1.
https://doi.org/10.1007/s13762-022-04405-3

Mandić N, Lalević B, Raičević V, Radojičić V. Impact of composting conditions on the nicotine degradation rate using nicotinophilic bacteria from tobacco waste. in International Journal of Environmental Science and Technology. 2022;1.
doi:10.1007/s13762-022-04405-3 .

Mandić, N., Lalević, B., Raičević, V., Radojičić, V., "Impact of composting conditions on the nicotine degradation rate using nicotinophilic bacteria from tobacco waste" in International Journal of Environmental Science and Technology, 1 (2022),
https://doi.org/10.1007/s13762-022-04405-3 . .

TY  - CONF
AU  - Kljujev, Igor
AU  - Jovicic-Petrovic, Jelena
AU  - Lalevic, Blazo
AU  - Karlicic, Vera
AU  - Todorovic, Irena
AU  - Prijepoljac, Minela
AU  - Raicevic, Vera
PY  - 2021
UR  - https://iwa-network.org/events/12th-eastern-european-young-water-professionals-conference-water-for-all-water-for-nature-reliable-water-supply-wastewater-treatment/#:~:text=In%202021%20we%20organize%20our,it%20will%20not%20be%20possible.
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6577
AB  - Surface water contamination becomes a raising problem due to different anthropogenic factors such as agriculture, industry, waste landfills, and wastewater effluents. Small rivers are also exposed to wastewater influents, pollution from agricultural activities, as well as raising human influence on the river flow (dam building). This research aimed to analyze the microbiological quality and ecological status of the water on the small river Ribnica in Western Serbia. Ribnica is a 22 km long tributary of Kolubara. The upper part of the river flow is surrounded by forests, agricultural fields, and small farms. Downstream, the river flows through Mionica, a small town where its water quality becomes endangered by communal wastewater, as well as landfills in the river surrounding. The microbiological quality of river water was estimated by the presence of coliforms, E. coli, enterococci which determination was done by Colilert and Enterolert method (IDEXX Laboratories, USA). The other parameters for water ecological status (oligotrophs, heterotrophs microorganisms, and autopurification index) are determined using the serial dilution method and incubation on selective nutrient media. The index of autopurification was calculated as oligotrophs/heterotrophs ratio. Our results show that the greatest pollution comes in the river after Mionica center. The extremely large number of fecal E. coli indicates the presence of fecal matter in water which could be a potential risk for human health if this water is used for irrigation crops or recreation. Also, autopurification index of water in this location indicates very poor self-purification potential which means that this water needs human help for achieving good ecological status. Regular monitoring and analyses of microbiological contamination and ecosystem disturbances should be important input data for public policies and strategic planning in the area of wastewater management, hydraulic engineering, and agricultural management.
C3  - 12th Eastern European Young Water Professionals Conference IWA
T1  - Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6577
ER  - 

@conference{
author = "Kljujev, Igor and Jovicic-Petrovic, Jelena and Lalevic, Blazo and Karlicic, Vera and Todorovic, Irena and Prijepoljac, Minela and Raicevic, Vera",
year = "2021",
abstract = "Surface water contamination becomes a raising problem due to different anthropogenic factors such as agriculture, industry, waste landfills, and wastewater effluents. Small rivers are also exposed to wastewater influents, pollution from agricultural activities, as well as raising human influence on the river flow (dam building). This research aimed to analyze the microbiological quality and ecological status of the water on the small river Ribnica in Western Serbia. Ribnica is a 22 km long tributary of Kolubara. The upper part of the river flow is surrounded by forests, agricultural fields, and small farms. Downstream, the river flows through Mionica, a small town where its water quality becomes endangered by communal wastewater, as well as landfills in the river surrounding. The microbiological quality of river water was estimated by the presence of coliforms, E. coli, enterococci which determination was done by Colilert and Enterolert method (IDEXX Laboratories, USA). The other parameters for water ecological status (oligotrophs, heterotrophs microorganisms, and autopurification index) are determined using the serial dilution method and incubation on selective nutrient media. The index of autopurification was calculated as oligotrophs/heterotrophs ratio. Our results show that the greatest pollution comes in the river after Mionica center. The extremely large number of fecal E. coli indicates the presence of fecal matter in water which could be a potential risk for human health if this water is used for irrigation crops or recreation. Also, autopurification index of water in this location indicates very poor self-purification potential which means that this water needs human help for achieving good ecological status. Regular monitoring and analyses of microbiological contamination and ecosystem disturbances should be important input data for public policies and strategic planning in the area of wastewater management, hydraulic engineering, and agricultural management.",
journal = "12th Eastern European Young Water Professionals Conference IWA",
title = "Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6577"
}

Kljujev, I., Jovicic-Petrovic, J., Lalevic, B., Karlicic, V., Todorovic, I., Prijepoljac, M.,& Raicevic, V.. (2021). Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water. in 12th Eastern European Young Water Professionals Conference IWA.
https://hdl.handle.net/21.15107/rcub_agrospace_6577

Kljujev I, Jovicic-Petrovic J, Lalevic B, Karlicic V, Todorovic I, Prijepoljac M, Raicevic V. Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water. in 12th Eastern European Young Water Professionals Conference IWA. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6577 .

Kljujev, Igor, Jovicic-Petrovic, Jelena, Lalevic, Blazo, Karlicic, Vera, Todorovic, Irena, Prijepoljac, Minela, Raicevic, Vera, "Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water" in 12th Eastern European Young Water Professionals Conference IWA (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6577 .

TY  - CONF
AU  - Kerečki, Slavica
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Karličič, Vera
AU  - Petrović, Ivana
AU  - Raičević, Vera
PY  - 2021
UR  - https://agrosym.ues.rs.ba/article/showpdf/BOOK_OF_PROCEEDINGS_2021_FINAL.pdf
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6578
AB  - Crop yields are highly depended on germination and early stages of plant growth. Numerous
priming techniques are being developed aimed to improve germination. Biopriming represents a
sustainable approach based on seed treatment in bacterial suspension of selected plant growth
promoting strains. One of the most promising plant growth promoting bacteria is Azotobacter
chroococcum. The aim of the research was to evaluate the effects of A. chroococcum F8/2 as a
biopriming agent on germination of various cultivable plants: basil, white mustard, cucumber,
tomato, wheat, canola, and soybean. After surface sterilization, seeds were bioprimed in the
bacterial suspension (107CFU/ml). Uninoculated seeds represented control treatment.
Germination test was conducted with 100 seeds per treatment and the germination was
monitored for 7 days. Following germination parameters were determined: germination
percentage, germination index, mean germination time, vigor I, vigor II, length and dry biomass
of the seedlings. The bacterial inoculation caused higher germination percentages of cucumber,
tomato, wheat and soybean. The highest increase in germination index was observed in wheat
(an increase of 19.8%). Tomato and basil were the only plants where vigor I was not increased
by inoculation. Generally, the most favorable effects of A. chroococcum biopriming were
observed in wheat where vigor I was increased more than twice, and vigor II was higher by
75.4% in inoculated seeds. The results indicate a significant potential for A. chroococcum use in
biopriming. The observed effects of seed priming on germination parameters were crop-specific,
with the most prominent potential in wheat biopriming.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy
PB  - International Society of Environment and Rural Development, Japan
PB  - ...
C3  - Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
T1  - BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6578
ER  - 

@conference{
author = "Kerečki, Slavica and Jovičić-Petrović, Jelena and Kljujev, Igor and Lalević, Blažo and Karličič, Vera and Petrović, Ivana and Raičević, Vera",
year = "2021",
abstract = "Crop yields are highly depended on germination and early stages of plant growth. Numerous
priming techniques are being developed aimed to improve germination. Biopriming represents a
sustainable approach based on seed treatment in bacterial suspension of selected plant growth
promoting strains. One of the most promising plant growth promoting bacteria is Azotobacter
chroococcum. The aim of the research was to evaluate the effects of A. chroococcum F8/2 as a
biopriming agent on germination of various cultivable plants: basil, white mustard, cucumber,
tomato, wheat, canola, and soybean. After surface sterilization, seeds were bioprimed in the
bacterial suspension (107CFU/ml). Uninoculated seeds represented control treatment.
Germination test was conducted with 100 seeds per treatment and the germination was
monitored for 7 days. Following germination parameters were determined: germination
percentage, germination index, mean germination time, vigor I, vigor II, length and dry biomass
of the seedlings. The bacterial inoculation caused higher germination percentages of cucumber,
tomato, wheat and soybean. The highest increase in germination index was observed in wheat
(an increase of 19.8%). Tomato and basil were the only plants where vigor I was not increased
by inoculation. Generally, the most favorable effects of A. chroococcum biopriming were
observed in wheat where vigor I was increased more than twice, and vigor II was higher by
75.4% in inoculated seeds. The results indicate a significant potential for A. chroococcum use in
biopriming. The observed effects of seed priming on germination parameters were crop-specific,
with the most prominent potential in wheat biopriming.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, International Society of Environment and Rural Development, Japan, ...",
journal = "Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”",
title = "BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6578"
}

Kerečki, S., Jovičić-Petrović, J., Kljujev, I., Lalević, B., Karličič, V., Petrović, I.,& Raičević, V.. (2021). BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6578

Kerečki S, Jovičić-Petrović J, Kljujev I, Lalević B, Karličič V, Petrović I, Raičević V. BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6578 .

Kerečki, Slavica, Jovičić-Petrović, Jelena, Kljujev, Igor, Lalević, Blažo, Karličič, Vera, Petrović, Ivana, Raičević, Vera, "BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT" in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021” (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6578 .

TY  - CONF
AU  - Kljujev, Igor
AU  - Karličič, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Veličković, Ana
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6602
AB  - Good microbiological quality of irrigation water has a great importance for health-safety
agricultural production. In order to avoid microbiological contamination of crops, the
microbiological quality of the water supplying the crops should be monitored. The aim of this
paper is investigation of the microbiological quality of the water from 5 channels and one pool
from the municipality of Surcin which can potentially be used for irrigation, as well as
determination the microbiological quality of the vegetables which are irrigated from the pool.
Microbiological parameters of water and vegetables quality are determined by the standard
methods prescribed by the Rulebook on parameters of surface waters (MPN method) and
Rulebook of food hygiene of production of Republic of Serbia. The values of the tested
parameters in one of the five investigated channels, Surcin channel water, were higher in relation
to the limit values of the Rulebook. The increased levels of total and fecal coliforms, Escherichia
coli, Salmonella, and Shigella were found. The Surcin channel water requires appropriate
treatment before its use for crop irrigation. The values of other tested waters did not go beyond
the limit values prescribed by the Rulebook, but for the safer agricultural production their
treatment is recommended. Microbiological analyzes of vegetables were also done. The total and
fecal coliform bacteria, as well as E. coli, Salmonella sp. and Shigella sp., were not detected in
the tested vegetable samples.
C3  - Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
T1  - MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION
EP  - 529
SP  - 523
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6602
ER  - 

@conference{
author = "Kljujev, Igor and Karličič, Vera and Jovičić-Petrović, Jelena and Veličković, Ana and Lalević, Blažo and Raičević, Vera",
year = "2021",
abstract = "Good microbiological quality of irrigation water has a great importance for health-safety
agricultural production. In order to avoid microbiological contamination of crops, the
microbiological quality of the water supplying the crops should be monitored. The aim of this
paper is investigation of the microbiological quality of the water from 5 channels and one pool
from the municipality of Surcin which can potentially be used for irrigation, as well as
determination the microbiological quality of the vegetables which are irrigated from the pool.
Microbiological parameters of water and vegetables quality are determined by the standard
methods prescribed by the Rulebook on parameters of surface waters (MPN method) and
Rulebook of food hygiene of production of Republic of Serbia. The values of the tested
parameters in one of the five investigated channels, Surcin channel water, were higher in relation
to the limit values of the Rulebook. The increased levels of total and fecal coliforms, Escherichia
coli, Salmonella, and Shigella were found. The Surcin channel water requires appropriate
treatment before its use for crop irrigation. The values of other tested waters did not go beyond
the limit values prescribed by the Rulebook, but for the safer agricultural production their
treatment is recommended. Microbiological analyzes of vegetables were also done. The total and
fecal coliform bacteria, as well as E. coli, Salmonella sp. and Shigella sp., were not detected in
the tested vegetable samples.",
journal = "Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”",
title = "MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION",
pages = "529-523",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6602"
}

Kljujev, I., Karličič, V., Jovičić-Petrović, J., Veličković, A., Lalević, B.,& Raičević, V.. (2021). MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, 523-529.
https://hdl.handle.net/21.15107/rcub_agrospace_6602

Kljujev I, Karličič V, Jovičić-Petrović J, Veličković A, Lalević B, Raičević V. MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”. 2021;:523-529.
https://hdl.handle.net/21.15107/rcub_agrospace_6602 .

Kljujev, Igor, Karličič, Vera, Jovičić-Petrović, Jelena, Veličković, Ana, Lalević, Blažo, Raičević, Vera, "MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION" in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021” (2021):523-529,
https://hdl.handle.net/21.15107/rcub_agrospace_6602 .

TY  - JOUR
AU  - Jovičić-Petrović, Jelena
AU  - Karličić, Vera
AU  - Petrović, Ivana
AU  - Ćirković, Saša
AU  - Ristić‐Đurović, Jasna L.
AU  - Raičević, Vera
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5802
AB  - Different priming methods were developed to improve seed germination and the early growth of seedlings. This study aimed to examine the combined effect of bacterial inoculation and static magnetic field on white mustard (Sinapis alba L.) germination. A plant growth‐promoting bacterial strain Bacillus amyloliquefaciens D5 ARV was used for biopriming. The static magnetic field of 90 mT was applied for 5 and 15 min. Analyses of abscisic acid, chlorophyll, anthocyanins, flavonoids content, nitrogen balance index, and bacterial indole‐3‐acetic acid were used to explain observed effects. Bacterial inoculation improved seed germination, whereas exposure to 90 mT for 15 min suppressed germination. Such an unfavorable effect was neutralized when the treatment with the static magnetic field was combined with bacterial inoculation. The highest germination percentage was a result of synergistic action of B. amyloliquefaciens D5 ARV and 15 min long exposure to 90 mT, which induced an increase of 53.20% in the number of germinated seeds. The static magnetic field induced the increase of bacterial indole‐3‐acetic acid production threefold times. Biomagnetic priming caused a metabolic shift from primary to secondary metabolism in the white mustard seedlings. An adequate combination of biological priming and static magnetic field treatment can be successfully used in old seed revitalization and germination improvements.
PB  - Wiley Online Library
T2  - Bio Electro Magnetics
T1  - Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds
DO  - 10.1002/bem.22328
ER  - 

@article{
author = "Jovičić-Petrović, Jelena and Karličić, Vera and Petrović, Ivana and Ćirković, Saša and Ristić‐Đurović, Jasna L. and Raičević, Vera",
year = "2021",
abstract = "Different priming methods were developed to improve seed germination and the early growth of seedlings. This study aimed to examine the combined effect of bacterial inoculation and static magnetic field on white mustard (Sinapis alba L.) germination. A plant growth‐promoting bacterial strain Bacillus amyloliquefaciens D5 ARV was used for biopriming. The static magnetic field of 90 mT was applied for 5 and 15 min. Analyses of abscisic acid, chlorophyll, anthocyanins, flavonoids content, nitrogen balance index, and bacterial indole‐3‐acetic acid were used to explain observed effects. Bacterial inoculation improved seed germination, whereas exposure to 90 mT for 15 min suppressed germination. Such an unfavorable effect was neutralized when the treatment with the static magnetic field was combined with bacterial inoculation. The highest germination percentage was a result of synergistic action of B. amyloliquefaciens D5 ARV and 15 min long exposure to 90 mT, which induced an increase of 53.20% in the number of germinated seeds. The static magnetic field induced the increase of bacterial indole‐3‐acetic acid production threefold times. Biomagnetic priming caused a metabolic shift from primary to secondary metabolism in the white mustard seedlings. An adequate combination of biological priming and static magnetic field treatment can be successfully used in old seed revitalization and germination improvements.",
publisher = "Wiley Online Library",
journal = "Bio Electro Magnetics",
title = "Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds",
doi = "10.1002/bem.22328"
}

Jovičić-Petrović, J., Karličić, V., Petrović, I., Ćirković, S., Ristić‐Đurović, J. L.,& Raičević, V.. (2021). Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds. in Bio Electro Magnetics
Wiley Online Library..
https://doi.org/10.1002/bem.22328

Jovičić-Petrović J, Karličić V, Petrović I, Ćirković S, Ristić‐Đurović JL, Raičević V. Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds. in Bio Electro Magnetics. 2021;.
doi:10.1002/bem.22328 .

Jovičić-Petrović, Jelena, Karličić, Vera, Petrović, Ivana, Ćirković, Saša, Ristić‐Đurović, Jasna L., Raičević, Vera, "Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds" in Bio Electro Magnetics (2021),
https://doi.org/10.1002/bem.22328 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Zlatković, Milica
AU  - Jovičić-Petrović, Jelena
AU  - Nikolić, Milan P.
AU  - Orlović, Saša
AU  - Raičević, Vera
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6021
AB  - Pinus sylvestris bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of P. sylvestris bark against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Botryosphaeriaceae) through its chemical (water extracts) and biological (Trichoderma spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of B. dothidea and D. sarmentorum for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three Trichoderma isolates originating from the pine bark. Trichoderma spp. reduced growth of B. dothidea by 67%–85%, D. sarmentorum by 63%–75% and N. parvum by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of P. sylvestris bark extracts and Trichoderma spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control.
PB  - MDPI
T2  - Forests
T1  - Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae
IS  - 12
SP  - 1731
VL  - 12
DO  - 10.3390/f12121731
ER  - 

@article{
author = "Karličić, Vera and Zlatković, Milica and Jovičić-Petrović, Jelena and Nikolić, Milan P. and Orlović, Saša and Raičević, Vera",
year = "2021",
abstract = "Pinus sylvestris bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of P. sylvestris bark against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Botryosphaeriaceae) through its chemical (water extracts) and biological (Trichoderma spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of B. dothidea and D. sarmentorum for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three Trichoderma isolates originating from the pine bark. Trichoderma spp. reduced growth of B. dothidea by 67%–85%, D. sarmentorum by 63%–75% and N. parvum by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of P. sylvestris bark extracts and Trichoderma spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control.",
publisher = "MDPI",
journal = "Forests",
title = "Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae",
number = "12",
pages = "1731",
volume = "12",
doi = "10.3390/f12121731"
}

Karličić, V., Zlatković, M., Jovičić-Petrović, J., Nikolić, M. P., Orlović, S.,& Raičević, V.. (2021). Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae. in Forests
MDPI., 12(12), 1731.
https://doi.org/10.3390/f12121731

Karličić V, Zlatković M, Jovičić-Petrović J, Nikolić MP, Orlović S, Raičević V. Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae. in Forests. 2021;12(12):1731.
doi:10.3390/f12121731 .

Karličić, Vera, Zlatković, Milica, Jovičić-Petrović, Jelena, Nikolić, Milan P., Orlović, Saša, Raičević, Vera, "Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae" in Forests, 12, no. 12 (2021):1731,
https://doi.org/10.3390/f12121731 . .

TY  - JOUR
AU  - Dragojević, Milica
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6842
AB  - BACKGROUND
Determination of the halophytic microbiome diversity is the basis for understanding the mechanisms that enable halophytes to tolerate the increased salt concentration. This report provides new data regarding the bacterial halophyte community and diversity of the endorhizosphere of the halophytic Hordeum hystrix Roth. Hordeum hystrix Roth. (Poaceae) is an annual plant of seasonally flooded saline lands and consequently, plant samples were collected
from the area of the naturally formed saline lands, Slano Kopovo.

OBJECTIVES
In addition to the analysis of microbiome diversity, the aim of this study was to characterize isolates of halophilic endophytic bacteria and their plant growth promoting ability.
METHODS
The metagenomic approach based on 16s rRNA gene sequencing was used to gain insight into the composition of the endophyte bacterial community at different taxonomic levels. The endophytic isolates were initially selected for the maximum salt concentration tolerance and the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Selected strains were tested on eight PGP traits at different NaCl concentrations.
RESULTS
Most isolates could tolerate the NaCl concentration over a range from 3-20%. Two strains showed positive results related to all eight tested PGP traits. At the high NaCl concentrations, the same strains retained seven positive PGP features. Our results indicate that the endorhizosphere of the halophytes is a source of halophilic PGP bacteria that, in addition to its beneficial effects on the host, have the possibility of their potential application in sustainable agriculture.
PB  - Federation of European Microbiological Societies, FEMS
T2  - FEMS Online Conference on Microbiology
T1  - MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6842
ER  - 

@article{
author = "Dragojević, Milica and Jovičić-Petrović, Jelena and Raičević, Vera",
year = "2020",
abstract = "BACKGROUND
Determination of the halophytic microbiome diversity is the basis for understanding the mechanisms that enable halophytes to tolerate the increased salt concentration. This report provides new data regarding the bacterial halophyte community and diversity of the endorhizosphere of the halophytic Hordeum hystrix Roth. Hordeum hystrix Roth. (Poaceae) is an annual plant of seasonally flooded saline lands and consequently, plant samples were collected
from the area of the naturally formed saline lands, Slano Kopovo.

OBJECTIVES
In addition to the analysis of microbiome diversity, the aim of this study was to characterize isolates of halophilic endophytic bacteria and their plant growth promoting ability.
METHODS
The metagenomic approach based on 16s rRNA gene sequencing was used to gain insight into the composition of the endophyte bacterial community at different taxonomic levels. The endophytic isolates were initially selected for the maximum salt concentration tolerance and the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Selected strains were tested on eight PGP traits at different NaCl concentrations.
RESULTS
Most isolates could tolerate the NaCl concentration over a range from 3-20%. Two strains showed positive results related to all eight tested PGP traits. At the high NaCl concentrations, the same strains retained seven positive PGP features. Our results indicate that the endorhizosphere of the halophytes is a source of halophilic PGP bacteria that, in addition to its beneficial effects on the host, have the possibility of their potential application in sustainable agriculture.",
publisher = "Federation of European Microbiological Societies, FEMS",
journal = "FEMS Online Conference on Microbiology",
title = "MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6842"
}

Dragojević, M., Jovičić-Petrović, J.,& Raičević, V.. (2020). MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies, FEMS..
https://hdl.handle.net/21.15107/rcub_agrospace_6842

Dragojević M, Jovičić-Petrović J, Raičević V. MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA. in FEMS Online Conference on Microbiology. 2020;.
https://hdl.handle.net/21.15107/rcub_agrospace_6842 .

Dragojević, Milica, Jovičić-Petrović, Jelena, Raičević, Vera, "MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA" in FEMS Online Conference on Microbiology (2020),
https://hdl.handle.net/21.15107/rcub_agrospace_6842 .

TY  - JOUR
AU  - Hamidović, Saud
AU  - Gojgić-Cvijović, Gordana
AU  - Waisi, Hadi K.
AU  - Životić, Ljubomir
AU  - Janković-Šoja, Svjetlana
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5380
AB  - Surface mining activities, despite their benefits, lead to the deterioration of local and regional environmental quality and play a role in global ecosystem pollution. This research aimed to estimate the culturable microbial population structure at five locations near the opencast coal mine "Kakanj" (Bosnia and Herzegovina) via agar plate and phospholipid fatty acids (PLFA) method and to establish its relationship to the physical and chemical properties of soil. Using the ICP-OES method, the heavy metal pollution of all examined locations (overburden, former grass yard, forest, arable soil, and greenhouse) was observed. Substantial variations among the sites regarding the most expressed indicators of heavy metal pollution were noted; Cr, Pb, Ni, and Cu content ranged from 63.17 to 524.47, 20.57 to 349.47, 139.13 to 2785.67, and 25.97 to 458.73 mg/kg, respectively. In the overburden sample, considerable low microbial activity was detected; the bacterial count was approximately 6- to 18-fold lower in comparison with the other samples. PLFA analysis showed the reduction of microbial diversity, reflected through the prevalence of normal and branched saturated fatty acids, their ratio (ranged from 0.92 to 7.13), and the absence of fungal marker 18:2 omega 6 fatty acid. The principal component analysis showed a strong negative impact of heavy metals Na and B on main microbial and PLFA profiles. In contrast, stock of main chemical parameters, including Ca, K, Fe, and pH, was positively correlated with the microbial community structure.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Response of microbial community composition in soils affected by coal mine exploitation
IS  - 6
VL  - 192
DO  - 10.1007/s10661-020-08305-2
ER  - 

@article{
author = "Hamidović, Saud and Gojgić-Cvijović, Gordana and Waisi, Hadi K. and Životić, Ljubomir and Janković-Šoja, Svjetlana and Raičević, Vera and Lalević, Blažo",
year = "2020",
abstract = "Surface mining activities, despite their benefits, lead to the deterioration of local and regional environmental quality and play a role in global ecosystem pollution. This research aimed to estimate the culturable microbial population structure at five locations near the opencast coal mine "Kakanj" (Bosnia and Herzegovina) via agar plate and phospholipid fatty acids (PLFA) method and to establish its relationship to the physical and chemical properties of soil. Using the ICP-OES method, the heavy metal pollution of all examined locations (overburden, former grass yard, forest, arable soil, and greenhouse) was observed. Substantial variations among the sites regarding the most expressed indicators of heavy metal pollution were noted; Cr, Pb, Ni, and Cu content ranged from 63.17 to 524.47, 20.57 to 349.47, 139.13 to 2785.67, and 25.97 to 458.73 mg/kg, respectively. In the overburden sample, considerable low microbial activity was detected; the bacterial count was approximately 6- to 18-fold lower in comparison with the other samples. PLFA analysis showed the reduction of microbial diversity, reflected through the prevalence of normal and branched saturated fatty acids, their ratio (ranged from 0.92 to 7.13), and the absence of fungal marker 18:2 omega 6 fatty acid. The principal component analysis showed a strong negative impact of heavy metals Na and B on main microbial and PLFA profiles. In contrast, stock of main chemical parameters, including Ca, K, Fe, and pH, was positively correlated with the microbial community structure.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Response of microbial community composition in soils affected by coal mine exploitation",
number = "6",
volume = "192",
doi = "10.1007/s10661-020-08305-2"
}

Hamidović, S., Gojgić-Cvijović, G., Waisi, H. K., Životić, L., Janković-Šoja, S., Raičević, V.,& Lalević, B.. (2020). Response of microbial community composition in soils affected by coal mine exploitation. in Environmental Monitoring and Assessment
Springer, Dordrecht., 192(6).
https://doi.org/10.1007/s10661-020-08305-2

Hamidović S, Gojgić-Cvijović G, Waisi HK, Životić L, Janković-Šoja S, Raičević V, Lalević B. Response of microbial community composition in soils affected by coal mine exploitation. in Environmental Monitoring and Assessment. 2020;192(6).
doi:10.1007/s10661-020-08305-2 .

Hamidović, Saud, Gojgić-Cvijović, Gordana, Waisi, Hadi K., Životić, Ljubomir, Janković-Šoja, Svjetlana, Raičević, Vera, Lalević, Blažo, "Response of microbial community composition in soils affected by coal mine exploitation" in Environmental Monitoring and Assessment, 192, no. 6 (2020),
https://doi.org/10.1007/s10661-020-08305-2 . .

TY  - CONF
AU  - Hamidović, Saud
AU  - Lalević, Blažo
AU  - Borovac, B.
AU  - Kazlagić, A.
AU  - Haseljić, S.
AU  - Raičević, Vera
AU  - Djikić, M.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5436
AB  - When a pesticide gets into soil, it takes part in a number of physical, chemical and biological processes that depend not only on the compound itself, but a number of other factors such as physical, chemical, and biological characteristics of soil and climatic factors. Microorganisms play an important role in pesticide degradation as they are able to utilize the biogenic elements from those compounds, as well as energy for their physiological processes. On the other hand, pesticides are more or less toxic substances that can have adverse effect on microbial populations and prevent their development, reduce their abundance, deplete their taxonomic complexity and create communities with a lower level of diversity and reduced physiological activity. The aim of this study was to analyze post nicosulfuron application dynamics of microbial populations’ activity in the soil. By standard methodology, soil pH value was determined as slightly acid to slightly alkaline, humus and available K content as moderate to high, and available P content as very low to moderate. Microbial abundance (total number of bacteria, number of ammonification bacteria, fungi and actinomycetes) was determined by agar plate method. The application of nicosulfuron in all samples and at both locations has resulted in increased number of bacteria, fungi and ammonification bacteria and reduction of the number of actinomycetes. The highest positive effect on the number of microorganisms was 15 days after treatment with nicosulfuron.
PB  - Springer
C3  - IFMBE Proceedings
T1  - Dynamics of microbial populations activities after the application of nicosulfuron
EP  - 280
SP  - 275
VL  - 78
DO  - 10.1007/978-3-030-40049-1_35
ER  - 

@conference{
author = "Hamidović, Saud and Lalević, Blažo and Borovac, B. and Kazlagić, A. and Haseljić, S. and Raičević, Vera and Djikić, M.",
year = "2020",
abstract = "When a pesticide gets into soil, it takes part in a number of physical, chemical and biological processes that depend not only on the compound itself, but a number of other factors such as physical, chemical, and biological characteristics of soil and climatic factors. Microorganisms play an important role in pesticide degradation as they are able to utilize the biogenic elements from those compounds, as well as energy for their physiological processes. On the other hand, pesticides are more or less toxic substances that can have adverse effect on microbial populations and prevent their development, reduce their abundance, deplete their taxonomic complexity and create communities with a lower level of diversity and reduced physiological activity. The aim of this study was to analyze post nicosulfuron application dynamics of microbial populations’ activity in the soil. By standard methodology, soil pH value was determined as slightly acid to slightly alkaline, humus and available K content as moderate to high, and available P content as very low to moderate. Microbial abundance (total number of bacteria, number of ammonification bacteria, fungi and actinomycetes) was determined by agar plate method. The application of nicosulfuron in all samples and at both locations has resulted in increased number of bacteria, fungi and ammonification bacteria and reduction of the number of actinomycetes. The highest positive effect on the number of microorganisms was 15 days after treatment with nicosulfuron.",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Dynamics of microbial populations activities after the application of nicosulfuron",
pages = "280-275",
volume = "78",
doi = "10.1007/978-3-030-40049-1_35"
}

Hamidović, S., Lalević, B., Borovac, B., Kazlagić, A., Haseljić, S., Raičević, V.,& Djikić, M.. (2020). Dynamics of microbial populations activities after the application of nicosulfuron. in IFMBE Proceedings
Springer., 78, 275-280.
https://doi.org/10.1007/978-3-030-40049-1_35

Hamidović S, Lalević B, Borovac B, Kazlagić A, Haseljić S, Raičević V, Djikić M. Dynamics of microbial populations activities after the application of nicosulfuron. in IFMBE Proceedings. 2020;78:275-280.
doi:10.1007/978-3-030-40049-1_35 .

Hamidović, Saud, Lalević, Blažo, Borovac, B., Kazlagić, A., Haseljić, S., Raičević, Vera, Djikić, M., "Dynamics of microbial populations activities after the application of nicosulfuron" in IFMBE Proceedings, 78 (2020):275-280,
https://doi.org/10.1007/978-3-030-40049-1_35 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5474
AB  - Red clover (Trifolium pratense L.) seeds were inoculated with several plant growth-promoting bacteria (PGPB) and sown in the substrate contaminated with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organometallic derivatives of tin (OT). The aim was to determine if selected PGPB strains can promote the growth of red clover in the substrate contaminated with several organic pollutants. The influence of bacteria on red clover growth (height, root length and biomass) was monitored during the three-month experimental period. The most significant improvements of seedling height were noted in the treatment with Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. Root growth was positively affected by Serratia liquefaciens Z-I ARV. The same isolates significantly affected biomass production. Those isolates caused total biomass increases of 70%, 48% and 33% compared to control. Bacterial strains used in this study were already confirmed as PGPB by biochemical testing, as well as by an in vivo test of mixed inoculums on several woody plants grown in the coal-mine overburden site. This work is the first-time record on their individual effects on one plant species. Obtained results confirmed that inoculation with several PGPB strains can enhance red clover growth in polluted soil.
AB  - Seme crvene deteline (Trifolium pratense L.), inokulisno sa nekoliko bakterija stimulatora biljnog rasta (PGPB), posejano je u supstrat kontaminiran policikličnim aromatičnim ugljovodonicima (PAHs), polihlorovanim bifenilima (PCBs) i organometalnim derivatima kalaja (OT). Cilj je bio da se utvrdi da li selektovane PGPB mogu promovisati rast crvene deteline u supstratu kontaminiranom sa nekoliko organskih zagađujućih materija. Uticaj bakterija na rast crvene deteline (visina, dužina korena i biomasa) praćen je tri meseca. Najveća visina je zabeležena kod biljaka inokulisanih sa Bacillus amyloliquefaciens D5 ARV i Pseudomonas putida P1 ARV. Rast korena je stimulisan od strane Serratia liquefaciens Z-I ARV. Ovi izolati su značajno uticali i na produkciju biomase. Ukupna biomasa dobijena tokom celog ogleda je za 70%, 48% i 33% veća u odnosu na kontrolu. Bakterijski sojevi korišćeni u ovoj studiji su prethodno potvrđeni kao PGPB kroz biohemijske i in vivo testove mešanog inokuluma na nekoliko drvenastih vrsta gajenih u jalovini. Ovaj rad prvi put beleži njihove pojedinačne efekte na jednu biljnu vrstu. Dobijeni rezultati potvrđuju da inokulacija sa nekoliko PGPB sojeva može ubrzati rast crvene deteline u zagađenom zemljištu.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Bacterial inoculation: A tool for red clover growth promotion in polluted soil
T1  - Bakterijska inokulacija - postupak za stimulaciju rasta crvene deteline gajene u zagađenom zemljištu
EP  - 174
IS  - 2
SP  - 163
VL  - 65
DO  - 10.2298/JAS2002163K
ER  - 

@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Raičević, Vera",
year = "2020",
abstract = "Red clover (Trifolium pratense L.) seeds were inoculated with several plant growth-promoting bacteria (PGPB) and sown in the substrate contaminated with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organometallic derivatives of tin (OT). The aim was to determine if selected PGPB strains can promote the growth of red clover in the substrate contaminated with several organic pollutants. The influence of bacteria on red clover growth (height, root length and biomass) was monitored during the three-month experimental period. The most significant improvements of seedling height were noted in the treatment with Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. Root growth was positively affected by Serratia liquefaciens Z-I ARV. The same isolates significantly affected biomass production. Those isolates caused total biomass increases of 70%, 48% and 33% compared to control. Bacterial strains used in this study were already confirmed as PGPB by biochemical testing, as well as by an in vivo test of mixed inoculums on several woody plants grown in the coal-mine overburden site. This work is the first-time record on their individual effects on one plant species. Obtained results confirmed that inoculation with several PGPB strains can enhance red clover growth in polluted soil., Seme crvene deteline (Trifolium pratense L.), inokulisno sa nekoliko bakterija stimulatora biljnog rasta (PGPB), posejano je u supstrat kontaminiran policikličnim aromatičnim ugljovodonicima (PAHs), polihlorovanim bifenilima (PCBs) i organometalnim derivatima kalaja (OT). Cilj je bio da se utvrdi da li selektovane PGPB mogu promovisati rast crvene deteline u supstratu kontaminiranom sa nekoliko organskih zagađujućih materija. Uticaj bakterija na rast crvene deteline (visina, dužina korena i biomasa) praćen je tri meseca. Najveća visina je zabeležena kod biljaka inokulisanih sa Bacillus amyloliquefaciens D5 ARV i Pseudomonas putida P1 ARV. Rast korena je stimulisan od strane Serratia liquefaciens Z-I ARV. Ovi izolati su značajno uticali i na produkciju biomase. Ukupna biomasa dobijena tokom celog ogleda je za 70%, 48% i 33% veća u odnosu na kontrolu. Bakterijski sojevi korišćeni u ovoj studiji su prethodno potvrđeni kao PGPB kroz biohemijske i in vivo testove mešanog inokuluma na nekoliko drvenastih vrsta gajenih u jalovini. Ovaj rad prvi put beleži njihove pojedinačne efekte na jednu biljnu vrstu. Dobijeni rezultati potvrđuju da inokulacija sa nekoliko PGPB sojeva može ubrzati rast crvene deteline u zagađenom zemljištu.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Bacterial inoculation: A tool for red clover growth promotion in polluted soil, Bakterijska inokulacija - postupak za stimulaciju rasta crvene deteline gajene u zagađenom zemljištu",
pages = "174-163",
number = "2",
volume = "65",
doi = "10.2298/JAS2002163K"
}

Karličić, V., Radić, D., Jovičić-Petrović, J.,& Raičević, V.. (2020). Bacterial inoculation: A tool for red clover growth promotion in polluted soil. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 65(2), 163-174.
https://doi.org/10.2298/JAS2002163K

Karličić V, Radić D, Jovičić-Petrović J, Raičević V. Bacterial inoculation: A tool for red clover growth promotion in polluted soil. in Journal of Agricultural Sciences (Belgrade). 2020;65(2):163-174.
doi:10.2298/JAS2002163K .

Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Raičević, Vera, "Bacterial inoculation: A tool for red clover growth promotion in polluted soil" in Journal of Agricultural Sciences (Belgrade), 65, no. 2 (2020):163-174,
https://doi.org/10.2298/JAS2002163K . .

TY  - JOUR
AU  - Ilić, Dora S.
AU  - Dimkić, Ivica
AU  - Waisi, Hadi K.
AU  - Gkorezis, Panagiotis M.
AU  - Hamidović, Saud
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4922
AB  - Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil
EP  - 258
IS  - 3
SP  - 247
VL  - 25
DO  - 10.2298/CICEQ180607003I
ER  - 

@article{
author = "Ilić, Dora S. and Dimkić, Ivica and Waisi, Hadi K. and Gkorezis, Panagiotis M. and Hamidović, Saud and Raičević, Vera and Lalević, Blažo",
year = "2019",
abstract = "Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil",
pages = "258-247",
number = "3",
volume = "25",
doi = "10.2298/CICEQ180607003I"
}

Ilić, D. S., Dimkić, I., Waisi, H. K., Gkorezis, P. M., Hamidović, S., Raičević, V.,& Lalević, B.. (2019). Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 25(3), 247-258.
https://doi.org/10.2298/CICEQ180607003I

Ilić DS, Dimkić I, Waisi HK, Gkorezis PM, Hamidović S, Raičević V, Lalević B. Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2019;25(3):247-258.
doi:10.2298/CICEQ180607003I .

Ilić, Dora S., Dimkić, Ivica, Waisi, Hadi K., Gkorezis, Panagiotis M., Hamidović, Saud, Raičević, Vera, Lalević, Blažo, "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 25, no. 3 (2019):247-258,
https://doi.org/10.2298/CICEQ180607003I . .