Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability
Само за регистроване кориснике
2023
Аутори
Karličič, VeraDragojević, Milica
Savić, Zagorka
Jovičić-Petrović, Jelena
Kljujev, Igor
Raičević, Vera
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation te...st revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.
Кључне речи:
Trichoderma spp. / biocontrol agents / Botrytis cinerea / Fusarium spp. / plant growth promotionИзвор:
ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE, 2023Институција/група
Poljoprivredni fakultetTY - CONF AU - Karličič, Vera AU - Dragojević, Milica AU - Savić, Zagorka AU - Jovičić-Petrović, Jelena AU - Kljujev, Igor AU - Raičević, Vera PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6606 AB - Modern agricultural practice is burdened with increasing caution related to the use of chemical inputs in the form of fertilizers and pesticides. These concerns have been followed by a growing interest in more ecological approaches to nutrient supplementation and disease control management. As an option, biofertilizers and biocontrol agents gather the scientific community, aiming to deeply explore and emphasize “the solutions from nature”. In that context, the objective of our research was to isolate Trichoderma spp. from different environments (agricultural, forest soil, and pine bark) and test their biocontrol and plant growth promoting potential. A total of 13 Trichoderma spp. were isolated, and confrontation tests were established with Botrytis cinerea, Fusarium graminearum, and Fusarium oxisporum. Biochemical characterization involved the assessment of several plant growth promoting characteristics (production of indoles, siderophores and enzymes). The confrontation test revealed different mechanisms employed in newly formed interactions. The mycelia of six Trichoderma isolates completely overgrew the tested pathogens, expressing very high antagonistic activity (≥75%), and emphasizing the competitive character of the isolates. The presence of an inhibition zone, a sign of antibiosis as a probable mode of action, was recorded within F. oxisporum interactions with several Trichoderma isolates. Biochemical characterization confirmed the production of cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase) which are correlated to mycoparasitism. Biochemical assessments confirmed the plant growth promoting nature of Trichoderma isolates, which were capable of producing indoles, siderophores and enzymes involved in P cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results suggests several newly isolated Trichoderma spp. as effective plant growth promotors capable of expressing direct and indirect mechanisms of growth stimulation and acting as promising biocontrol agents against significant crop pathogens. Further analyses demand assessment of field conditions performance and deeper insight into the mechanisms involved in mutualistic interactions with plants and antagonistic interactions with phytopathogens. C3 - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE T1 - Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability UR - https://hdl.handle.net/21.15107/rcub_agrospace_6606 ER -
@conference{ author = "Karličič, Vera and Dragojević, Milica and Savić, Zagorka and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera", year = "2023", abstract = "Modern agricultural practice is burdened with increasing caution related to the use of chemical inputs in the form of fertilizers and pesticides. These concerns have been followed by a growing interest in more ecological approaches to nutrient supplementation and disease control management. As an option, biofertilizers and biocontrol agents gather the scientific community, aiming to deeply explore and emphasize “the solutions from nature”. In that context, the objective of our research was to isolate Trichoderma spp. from different environments (agricultural, forest soil, and pine bark) and test their biocontrol and plant growth promoting potential. A total of 13 Trichoderma spp. were isolated, and confrontation tests were established with Botrytis cinerea, Fusarium graminearum, and Fusarium oxisporum. Biochemical characterization involved the assessment of several plant growth promoting characteristics (production of indoles, siderophores and enzymes). The confrontation test revealed different mechanisms employed in newly formed interactions. The mycelia of six Trichoderma isolates completely overgrew the tested pathogens, expressing very high antagonistic activity (≥75%), and emphasizing the competitive character of the isolates. The presence of an inhibition zone, a sign of antibiosis as a probable mode of action, was recorded within F. oxisporum interactions with several Trichoderma isolates. Biochemical characterization confirmed the production of cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase) which are correlated to mycoparasitism. Biochemical assessments confirmed the plant growth promoting nature of Trichoderma isolates, which were capable of producing indoles, siderophores and enzymes involved in P cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results suggests several newly isolated Trichoderma spp. as effective plant growth promotors capable of expressing direct and indirect mechanisms of growth stimulation and acting as promising biocontrol agents against significant crop pathogens. Further analyses demand assessment of field conditions performance and deeper insight into the mechanisms involved in mutualistic interactions with plants and antagonistic interactions with phytopathogens.", journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE", title = "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6606" }
Karličič, V., Dragojević, M., Savić, Z., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2023). Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. https://hdl.handle.net/21.15107/rcub_agrospace_6606
Karličič V, Dragojević M, Savić Z, Jovičić-Petrović J, Kljujev I, Raičević V. Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;. https://hdl.handle.net/21.15107/rcub_agrospace_6606 .
Karličič, Vera, Dragojević, Milica, Savić, Zagorka, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023), https://hdl.handle.net/21.15107/rcub_agrospace_6606 .