MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA
Само за регистроване кориснике
2020
Чланак у часопису (Објављена верзија)
Метаподаци
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BACKGROUND
Determination of the halophytic microbiome diversity is the basis for understanding the mechanisms that enable halophytes to tolerate the increased salt concentration. This report provides new data regarding the bacterial halophyte community and diversity of the endorhizosphere of the halophytic Hordeum hystrix Roth. Hordeum hystrix Roth. (Poaceae) is an annual plant of seasonally flooded saline lands and consequently, plant samples were collected
from the area of the naturally formed saline lands, Slano Kopovo.
OBJECTIVES
In addition to the analysis of microbiome diversity, the aim of this study was to characterize isolates of halophilic endophytic bacteria and their plant growth promoting ability.
METHODS
The metagenomic approach based on 16s rRNA gene sequencing was used to gain insight into the composition of the endophyte bacterial community at different taxonomic levels. The endophytic isolates were initially selected for the maximum salt concentration toleranc...e and the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Selected strains were tested on eight PGP traits at different NaCl concentrations.
RESULTS
Most isolates could tolerate the NaCl concentration over a range from 3-20%. Two strains showed positive results related to all eight tested PGP traits. At the high NaCl concentrations, the same strains retained seven positive PGP features. Our results indicate that the endorhizosphere of the halophytes is a source of halophilic PGP bacteria that, in addition to its beneficial effects on the host, have the possibility of their potential application in sustainable agriculture.
Извор:
FEMS Online Conference on Microbiology, 2020Издавач:
- Federation of European Microbiological Societies, FEMS
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Dragojević, Milica AU - Jovičić-Petrović, Jelena AU - Raičević, Vera PY - 2020 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6842 AB - BACKGROUND Determination of the halophytic microbiome diversity is the basis for understanding the mechanisms that enable halophytes to tolerate the increased salt concentration. This report provides new data regarding the bacterial halophyte community and diversity of the endorhizosphere of the halophytic Hordeum hystrix Roth. Hordeum hystrix Roth. (Poaceae) is an annual plant of seasonally flooded saline lands and consequently, plant samples were collected from the area of the naturally formed saline lands, Slano Kopovo. OBJECTIVES In addition to the analysis of microbiome diversity, the aim of this study was to characterize isolates of halophilic endophytic bacteria and their plant growth promoting ability. METHODS The metagenomic approach based on 16s rRNA gene sequencing was used to gain insight into the composition of the endophyte bacterial community at different taxonomic levels. The endophytic isolates were initially selected for the maximum salt concentration tolerance and the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Selected strains were tested on eight PGP traits at different NaCl concentrations. RESULTS Most isolates could tolerate the NaCl concentration over a range from 3-20%. Two strains showed positive results related to all eight tested PGP traits. At the high NaCl concentrations, the same strains retained seven positive PGP features. Our results indicate that the endorhizosphere of the halophytes is a source of halophilic PGP bacteria that, in addition to its beneficial effects on the host, have the possibility of their potential application in sustainable agriculture. PB - Federation of European Microbiological Societies, FEMS T2 - FEMS Online Conference on Microbiology T1 - MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA UR - https://hdl.handle.net/21.15107/rcub_agrospace_6842 ER -
@article{ author = "Dragojević, Milica and Jovičić-Petrović, Jelena and Raičević, Vera", year = "2020", abstract = "BACKGROUND Determination of the halophytic microbiome diversity is the basis for understanding the mechanisms that enable halophytes to tolerate the increased salt concentration. This report provides new data regarding the bacterial halophyte community and diversity of the endorhizosphere of the halophytic Hordeum hystrix Roth. Hordeum hystrix Roth. (Poaceae) is an annual plant of seasonally flooded saline lands and consequently, plant samples were collected from the area of the naturally formed saline lands, Slano Kopovo. OBJECTIVES In addition to the analysis of microbiome diversity, the aim of this study was to characterize isolates of halophilic endophytic bacteria and their plant growth promoting ability. METHODS The metagenomic approach based on 16s rRNA gene sequencing was used to gain insight into the composition of the endophyte bacterial community at different taxonomic levels. The endophytic isolates were initially selected for the maximum salt concentration tolerance and the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Selected strains were tested on eight PGP traits at different NaCl concentrations. RESULTS Most isolates could tolerate the NaCl concentration over a range from 3-20%. Two strains showed positive results related to all eight tested PGP traits. At the high NaCl concentrations, the same strains retained seven positive PGP features. Our results indicate that the endorhizosphere of the halophytes is a source of halophilic PGP bacteria that, in addition to its beneficial effects on the host, have the possibility of their potential application in sustainable agriculture.", publisher = "Federation of European Microbiological Societies, FEMS", journal = "FEMS Online Conference on Microbiology", title = "MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6842" }
Dragojević, M., Jovičić-Petrović, J.,& Raičević, V.. (2020). MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA. in FEMS Online Conference on Microbiology Federation of European Microbiological Societies, FEMS.. https://hdl.handle.net/21.15107/rcub_agrospace_6842
Dragojević M, Jovičić-Petrović J, Raičević V. MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA. in FEMS Online Conference on Microbiology. 2020;. https://hdl.handle.net/21.15107/rcub_agrospace_6842 .
Dragojević, Milica, Jovičić-Petrović, Jelena, Raičević, Vera, "MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA" in FEMS Online Conference on Microbiology (2020), https://hdl.handle.net/21.15107/rcub_agrospace_6842 .