@conference{
author = "Karličič, Vera and Dragojević, Milica and Savić, Zagorka and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2023",
abstract = "Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6606"
}
