TY  - CONF
AU  - Dragojević, Milica
AU  - Đokić, Lidija
AU  - Stanković, Nada
AU  - Raičević, Vera
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6605
AB  - Increased soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.
C3  - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE
T1  - Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6605
ER  - 

@conference{
author = "Dragojević, Milica and Đokić, Lidija and Stanković, Nada and Raičević, Vera and Karličič, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "Increased soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6605"
}

Dragojević, M., Đokić, L., Stanković, N., Raičević, V., Karličič, V., Lalević, B.,& Jovičić-Petrović, J.. (2023). Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE.
https://hdl.handle.net/21.15107/rcub_agrospace_6605

Dragojević M, Đokić L, Stanković N, Raičević V, Karličič V, Lalević B, Jovičić-Petrović J. Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6605 .

Dragojević, Milica, Đokić, Lidija, Stanković, Nada, Raičević, Vera, Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6605 .

TY  - JOUR
AU  - Dragojević, Milica
AU  - Stankovic, Nada
AU  - Djokic, Lidija
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6316
AB  - The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils. © 2023, The Author(s).
T2  - Environmental Microbiome
T2  - Environmental Microbiome
T1  - Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria
IS  - 1
VL  - 18
DO  - 10.1186/s40793-023-00477-x
ER  - 

@article{
author = "Dragojević, Milica and Stankovic, Nada and Djokic, Lidija and Raičević, Vera and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils. © 2023, The Author(s).",
journal = "Environmental Microbiome, Environmental Microbiome",
title = "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria",
number = "1",
volume = "18",
doi = "10.1186/s40793-023-00477-x"
}

Dragojević, M., Stankovic, N., Djokic, L., Raičević, V.,& Jovičić-Petrović, J.. (2023). Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome, 18(1).
https://doi.org/10.1186/s40793-023-00477-x

Dragojević M, Stankovic N, Djokic L, Raičević V, Jovičić-Petrović J. Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome. 2023;18(1).
doi:10.1186/s40793-023-00477-x .

Dragojević, Milica, Stankovic, Nada, Djokic, Lidija, Raičević, Vera, Jovičić-Petrović, Jelena, "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria" in Environmental Microbiome, 18, no. 1 (2023),
https://doi.org/10.1186/s40793-023-00477-x . .

TY  - CONF
AU  - Karličič, Vera
AU  - Dragojević, Milica
AU  - Savić, Zagorka
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6606
AB  - Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.
C3  - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE
T1  - Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6606
ER  - 

@conference{
author = "Karličič, Vera and Dragojević, Milica and Savić, Zagorka and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2023",
abstract = "Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6606"
}

Karličič, V., Dragojević, M., Savić, Z., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2023). Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE.
https://hdl.handle.net/21.15107/rcub_agrospace_6606

Karličič V, Dragojević M, Savić Z, Jovičić-Petrović J, Kljujev I, Raičević V. Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

Karličič, Vera, Dragojević, Milica, Savić, Zagorka, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

TY  - JOUR
AU  - Dragojević, Milica
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6842
AB  - BACKGROUND
Determination of the halophytic microbiome diversity is the basis for understanding the mechanisms that enable halophytes to tolerate the increased salt concentration. This report provides new data regarding the bacterial halophyte community and diversity of the endorhizosphere of the halophytic Hordeum hystrix Roth. Hordeum hystrix Roth. (Poaceae) is an annual plant of seasonally flooded saline lands and consequently, plant samples were collected
from the area of the naturally formed saline lands, Slano Kopovo.

OBJECTIVES
In addition to the analysis of microbiome diversity, the aim of this study was to characterize isolates of halophilic endophytic bacteria and their plant growth promoting ability.
METHODS
The metagenomic approach based on 16s rRNA gene sequencing was used to gain insight into the composition of the endophyte bacterial community at different taxonomic levels. The endophytic isolates were initially selected for the maximum salt concentration tolerance and the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Selected strains were tested on eight PGP traits at different NaCl concentrations.
RESULTS
Most isolates could tolerate the NaCl concentration over a range from 3-20%. Two strains showed positive results related to all eight tested PGP traits. At the high NaCl concentrations, the same strains retained seven positive PGP features. Our results indicate that the endorhizosphere of the halophytes is a source of halophilic PGP bacteria that, in addition to its beneficial effects on the host, have the possibility of their potential application in sustainable agriculture.
PB  - Federation of European Microbiological Societies, FEMS
T2  - FEMS Online Conference on Microbiology
T1  - MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6842
ER  - 

@article{
author = "Dragojević, Milica and Jovičić-Petrović, Jelena and Raičević, Vera",
year = "2020",
abstract = "BACKGROUND
Determination of the halophytic microbiome diversity is the basis for understanding the mechanisms that enable halophytes to tolerate the increased salt concentration. This report provides new data regarding the bacterial halophyte community and diversity of the endorhizosphere of the halophytic Hordeum hystrix Roth. Hordeum hystrix Roth. (Poaceae) is an annual plant of seasonally flooded saline lands and consequently, plant samples were collected
from the area of the naturally formed saline lands, Slano Kopovo.

OBJECTIVES
In addition to the analysis of microbiome diversity, the aim of this study was to characterize isolates of halophilic endophytic bacteria and their plant growth promoting ability.
METHODS
The metagenomic approach based on 16s rRNA gene sequencing was used to gain insight into the composition of the endophyte bacterial community at different taxonomic levels. The endophytic isolates were initially selected for the maximum salt concentration tolerance and the ability to produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Selected strains were tested on eight PGP traits at different NaCl concentrations.
RESULTS
Most isolates could tolerate the NaCl concentration over a range from 3-20%. Two strains showed positive results related to all eight tested PGP traits. At the high NaCl concentrations, the same strains retained seven positive PGP features. Our results indicate that the endorhizosphere of the halophytes is a source of halophilic PGP bacteria that, in addition to its beneficial effects on the host, have the possibility of their potential application in sustainable agriculture.",
publisher = "Federation of European Microbiological Societies, FEMS",
journal = "FEMS Online Conference on Microbiology",
title = "MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6842"
}

Dragojević, M., Jovičić-Petrović, J.,& Raičević, V.. (2020). MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies, FEMS..
https://hdl.handle.net/21.15107/rcub_agrospace_6842

Dragojević M, Jovičić-Petrović J, Raičević V. MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA. in FEMS Online Conference on Microbiology. 2020;.
https://hdl.handle.net/21.15107/rcub_agrospace_6842 .

Dragojević, Milica, Jovičić-Petrović, Jelena, Raičević, Vera, "MICROBIOME OF THE HALOPHYTE HORDEUM HYSTRIX ROTH. AS A SOURCE OF THE HALOPHILIC PGP BACTERIA" in FEMS Online Conference on Microbiology (2020),
https://hdl.handle.net/21.15107/rcub_agrospace_6842 .