TY  - JOUR
AU  - Duduk, Nataša
AU  - Vico, Ivana
AU  - Kosovac, Andrea
AU  - Stepanović, Jelena
AU  - Ćurčić, Živko
AU  - Vučković, Nina
AU  - Rekanović, Emil
AU  - Duduk, Bojan
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6347
AB  - ‘Candidatus Phytoplasma solani’ (stolbur phytoplasma) is associated with rubbery taproot disease (RTD) of sugar beet (Beta vulgaris L.), while Macrophomina phaseolina is considered the most important root rot pathogen of this plant in Serbia. The high prevalence of M. phaseolina root rot reported on sugar beet in Serbia, unmatched elsewhere in the world, coupled with the notorious tendency of RTD-affected sugar beet to rot, has prompted research into the relationship between the two diseases. This study investigates the correlation between the occurrence of sugar beet RTD and the presence of root rot fungal pathogens in a semi-field ‘Ca. P. solani’ transmission experiment with the cixiid vector Reptalus quinquecostatus (Dufour), in addition to naturally infected sugar beet in the open field. Our results showed that: (i) Reptalus quinquecostatus transmitted ‘Ca. P. solani’ to sugar beet which induced typical RTD root symptoms; (ii) Macrophomina phaseolina root rot was exclusively present in ‘Ca. P. solani’-infected sugar beet in both the semi-field experiment and naturally infected sugar beet; and that (iii) even under environmental conditions favorable to the pathogen, M. phaseolina did not infect sugar beet, unless the plants had been previously infected with phytoplasma. Copyright © 2023 Duduk, Vico, Kosovac, Stepanović, Ćurčić, Vučković, Rekanović and Duduk.
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - A biotroph sets the stage for a necrotroph to play: ‘Candidatus Phytoplasma solani’ infection of sugar beet facilitated Macrophomina phaseolina root rot
VL  - 14
DO  - 10.3389/fmicb.2023.1164035
ER  - 

@article{
author = "Duduk, Nataša and Vico, Ivana and Kosovac, Andrea and Stepanović, Jelena and Ćurčić, Živko and Vučković, Nina and Rekanović, Emil and Duduk, Bojan",
year = "2023",
abstract = "‘Candidatus Phytoplasma solani’ (stolbur phytoplasma) is associated with rubbery taproot disease (RTD) of sugar beet (Beta vulgaris L.), while Macrophomina phaseolina is considered the most important root rot pathogen of this plant in Serbia. The high prevalence of M. phaseolina root rot reported on sugar beet in Serbia, unmatched elsewhere in the world, coupled with the notorious tendency of RTD-affected sugar beet to rot, has prompted research into the relationship between the two diseases. This study investigates the correlation between the occurrence of sugar beet RTD and the presence of root rot fungal pathogens in a semi-field ‘Ca. P. solani’ transmission experiment with the cixiid vector Reptalus quinquecostatus (Dufour), in addition to naturally infected sugar beet in the open field. Our results showed that: (i) Reptalus quinquecostatus transmitted ‘Ca. P. solani’ to sugar beet which induced typical RTD root symptoms; (ii) Macrophomina phaseolina root rot was exclusively present in ‘Ca. P. solani’-infected sugar beet in both the semi-field experiment and naturally infected sugar beet; and that (iii) even under environmental conditions favorable to the pathogen, M. phaseolina did not infect sugar beet, unless the plants had been previously infected with phytoplasma. Copyright © 2023 Duduk, Vico, Kosovac, Stepanović, Ćurčić, Vučković, Rekanović and Duduk.",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "A biotroph sets the stage for a necrotroph to play: ‘Candidatus Phytoplasma solani’ infection of sugar beet facilitated Macrophomina phaseolina root rot",
volume = "14",
doi = "10.3389/fmicb.2023.1164035"
}

Duduk, N., Vico, I., Kosovac, A., Stepanović, J., Ćurčić, Ž., Vučković, N., Rekanović, E.,& Duduk, B.. (2023). A biotroph sets the stage for a necrotroph to play: ‘Candidatus Phytoplasma solani’ infection of sugar beet facilitated Macrophomina phaseolina root rot. in Frontiers in Microbiology, 14.
https://doi.org/10.3389/fmicb.2023.1164035

Duduk N, Vico I, Kosovac A, Stepanović J, Ćurčić Ž, Vučković N, Rekanović E, Duduk B. A biotroph sets the stage for a necrotroph to play: ‘Candidatus Phytoplasma solani’ infection of sugar beet facilitated Macrophomina phaseolina root rot. in Frontiers in Microbiology. 2023;14.
doi:10.3389/fmicb.2023.1164035 .

Duduk, Nataša, Vico, Ivana, Kosovac, Andrea, Stepanović, Jelena, Ćurčić, Živko, Vučković, Nina, Rekanović, Emil, Duduk, Bojan, "A biotroph sets the stage for a necrotroph to play: ‘Candidatus Phytoplasma solani’ infection of sugar beet facilitated Macrophomina phaseolina root rot" in Frontiers in Microbiology, 14 (2023),
https://doi.org/10.3389/fmicb.2023.1164035 . .

TY  - JOUR
AU  - Vučković, Nina
AU  - Vico, Ivana
AU  - Duduk, Nataša
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6300
T2  - Journal of Plant Pathology
T2  - Journal of Plant Pathology
T1  - First report of Botryosphaeria dothidea causing postharvest rot of quince fruits in Serbia
DO  - 10.1007/s42161-023-01314-3
ER  - 

@article{
author = "Vučković, Nina and Vico, Ivana and Duduk, Nataša",
year = "2023",
journal = "Journal of Plant Pathology, Journal of Plant Pathology",
title = "First report of Botryosphaeria dothidea causing postharvest rot of quince fruits in Serbia",
doi = "10.1007/s42161-023-01314-3"
}

Vučković, N., Vico, I.,& Duduk, N.. (2023). First report of Botryosphaeria dothidea causing postharvest rot of quince fruits in Serbia. in Journal of Plant Pathology.
https://doi.org/10.1007/s42161-023-01314-3

Vučković N, Vico I, Duduk N. First report of Botryosphaeria dothidea causing postharvest rot of quince fruits in Serbia. in Journal of Plant Pathology. 2023;.
doi:10.1007/s42161-023-01314-3 .

Vučković, Nina, Vico, Ivana, Duduk, Nataša, "First report of Botryosphaeria dothidea causing postharvest rot of quince fruits in Serbia" in Journal of Plant Pathology (2023),
https://doi.org/10.1007/s42161-023-01314-3 . .

TY  - JOUR
AU  - Vasić, Miljan
AU  - Vico, Ivana
AU  - Jurick, Wayne M.
AU  - Duduk, Bojan
AU  - Duduk, Nataša
PY  - 2022
UR  - https://doi.org/10.1007/s11557-022-01841-w
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6197
AB  - Lambertella corni-maris was isolated from a dark brown lesion on apple fruit cv. Golden Delicious collected from an orchard, and from stored apple fruit cv. Idared previously colonized with Monilia polystroma. The contrasting origin of the isolates led us to hypothesize that this fungus behaves differently in various contexts, which triggered the investigation of L. corni-maris as a pathogen and antagonist. Following identification based on cultural and molecular characteristics, isolates of different origin were characterized and their cultural, ecological, pathogenic, and antagonistic features were compared. This study has shown that L. corni-maris, originally isolated as an apple fruit pathogen and one as an antagonist of M. polystroma, have similar features in vitro and in vivo. The isolates vary widely in growth rate, cultural morphology, crystal production, intensity of dark zone and co-antagonism with two Monilinia species but can also function as apple fruit pathogens and inhibit and replace different Monilinia spp. on apple fruit. These findings reinforce their dual nature in different host-pathogen interactions. The data from this study are biologically intriguing and practically relevant, and have translative potential to exploit L. corni-maris as a model system for developing strategies to block brown rot of apple and to pinpoint virulence factors in the fungus for yellow rot management.
T2  - Mycological Progress
T2  - Mycological ProgressMycol Progress
T1  - The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp
IS  - 11
SP  - 91
VL  - 21
DO  - 10.1007/s11557-022-01841-w
ER  - 

@article{
author = "Vasić, Miljan and Vico, Ivana and Jurick, Wayne M. and Duduk, Bojan and Duduk, Nataša",
year = "2022",
abstract = "Lambertella corni-maris was isolated from a dark brown lesion on apple fruit cv. Golden Delicious collected from an orchard, and from stored apple fruit cv. Idared previously colonized with Monilia polystroma. The contrasting origin of the isolates led us to hypothesize that this fungus behaves differently in various contexts, which triggered the investigation of L. corni-maris as a pathogen and antagonist. Following identification based on cultural and molecular characteristics, isolates of different origin were characterized and their cultural, ecological, pathogenic, and antagonistic features were compared. This study has shown that L. corni-maris, originally isolated as an apple fruit pathogen and one as an antagonist of M. polystroma, have similar features in vitro and in vivo. The isolates vary widely in growth rate, cultural morphology, crystal production, intensity of dark zone and co-antagonism with two Monilinia species but can also function as apple fruit pathogens and inhibit and replace different Monilinia spp. on apple fruit. These findings reinforce their dual nature in different host-pathogen interactions. The data from this study are biologically intriguing and practically relevant, and have translative potential to exploit L. corni-maris as a model system for developing strategies to block brown rot of apple and to pinpoint virulence factors in the fungus for yellow rot management.",
journal = "Mycological Progress, Mycological ProgressMycol Progress",
title = "The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp",
number = "11",
pages = "91",
volume = "21",
doi = "10.1007/s11557-022-01841-w"
}

Vasić, M., Vico, I., Jurick, W. M., Duduk, B.,& Duduk, N.. (2022). The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp. in Mycological Progress, 21(11), 91.
https://doi.org/10.1007/s11557-022-01841-w

Vasić M, Vico I, Jurick WM, Duduk B, Duduk N. The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp. in Mycological Progress. 2022;21(11):91.
doi:10.1007/s11557-022-01841-w .

Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., Duduk, Bojan, Duduk, Nataša, "The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp" in Mycological Progress, 21, no. 11 (2022):91,
https://doi.org/10.1007/s11557-022-01841-w . .

TY  - JOUR
AU  - Žebeljan, Aleksandra
AU  - Duduk, Nataša
AU  - Vučković, Nina
AU  - Jurick II, Wayne M.
AU  - Vico, Ivana
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6020
AB  - Blue mold, caused by Penicillium spp., is one of the most economically important postharvest diseases of pome fruits, globally. Pome fruits, in particular apple, is the most widely grown pome fruit in Serbia, and the distribution of Penicillium spp. responsible for postharvest decay is unknown. A two-year survey was conducted in 2014 and 2015, where four pome fruits (apple, pear, quince, and medlar) with blue mold symptoms were collected from 20 storage locations throughout Serbia. Detailed morphological characterization, analysis of virulence in three apple cultivars, and multilocus phylogeny revealed three main Penicillium spp. in order of abundance: P. expansum, P. crustosum, and P. solitum. Interestingly, P. expansum split into two distinct clades with strong statistical support that coincided with several morphological observations. Findings from this study are significant and showed previously undocumented diversity in blue mold fungi responsible for postharvest decay including the first finding of P. crustosum, and P. solitum as postharvest pathogens of quince and P. crustosum of medlar fruit in the world, and P. expansum of quince in Serbia. Data from this study provide timely information regarding phenotypic, morphological and genotypic plasticity in P. expansum that will impact the design of species-specific detection tools and guide the development of blue mold management strategies.
PB  - MDPI
T2  - J. Fungi
T1  - Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia
IS  - 12
SP  - 1019
VL  - 7
DO  - 10.3390/jof7121019
ER  - 

@article{
author = "Žebeljan, Aleksandra and Duduk, Nataša and Vučković, Nina and Jurick II, Wayne M. and Vico, Ivana",
year = "2021",
abstract = "Blue mold, caused by Penicillium spp., is one of the most economically important postharvest diseases of pome fruits, globally. Pome fruits, in particular apple, is the most widely grown pome fruit in Serbia, and the distribution of Penicillium spp. responsible for postharvest decay is unknown. A two-year survey was conducted in 2014 and 2015, where four pome fruits (apple, pear, quince, and medlar) with blue mold symptoms were collected from 20 storage locations throughout Serbia. Detailed morphological characterization, analysis of virulence in three apple cultivars, and multilocus phylogeny revealed three main Penicillium spp. in order of abundance: P. expansum, P. crustosum, and P. solitum. Interestingly, P. expansum split into two distinct clades with strong statistical support that coincided with several morphological observations. Findings from this study are significant and showed previously undocumented diversity in blue mold fungi responsible for postharvest decay including the first finding of P. crustosum, and P. solitum as postharvest pathogens of quince and P. crustosum of medlar fruit in the world, and P. expansum of quince in Serbia. Data from this study provide timely information regarding phenotypic, morphological and genotypic plasticity in P. expansum that will impact the design of species-specific detection tools and guide the development of blue mold management strategies.",
publisher = "MDPI",
journal = "J. Fungi",
title = "Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia",
number = "12",
pages = "1019",
volume = "7",
doi = "10.3390/jof7121019"
}

Žebeljan, A., Duduk, N., Vučković, N., Jurick II, W. M.,& Vico, I.. (2021). Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia. in J. Fungi
MDPI., 7(12), 1019.
https://doi.org/10.3390/jof7121019

Žebeljan A, Duduk N, Vučković N, Jurick II WM, Vico I. Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia. in J. Fungi. 2021;7(12):1019.
doi:10.3390/jof7121019 .

Žebeljan, Aleksandra, Duduk, Nataša, Vučković, Nina, Jurick II, Wayne M., Vico, Ivana, "Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia" in J. Fungi, 7, no. 12 (2021):1019,
https://doi.org/10.3390/jof7121019 . .

TY  - JOUR
AU  - Mikić, Sara D.
AU  - Vico, Ivana M.
AU  - Duduk, Nataša D.
AU  - Marković, Tatjana Lj.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5852
AB  - Seed health testing aims to ensure a healthy crop and to prevent the spread of plant diseases. The need to study and apply both essential oils and plant extracts against seed diseases is growing and becoming more important. Coriander (Coriandrum sativum L.) is an annual herbaceous, aromatic, spicy and medicinal plant from the Apiaceae family, known for its seeds with a characteristic and pleasant odour. In human medicine, coriander is a part of various medicines for the treatment of digestive organs. Phytopathogenic fungi and bacteria are the most common and significant causes of coriander seed disease. The aim of this study was to assess coriander seed health and investigate the possibility of seed disinfection using peppermint (Mentha x piperita) essential oil. The health of the seeds was examined by the filter paper laboratory standard method. Different concentrations (1, 2, 5 and 10%) of peppermint essential oil were used for seed disinfection. The composition of the essential oil was determined by gas chromatographic analysis. The results of seed health analysis showed the presence of a fungus after seven days of incubation in a humid chamber at room temperature. The fungus was identified as Alternaria sp. (sect. Alternaria) based on the symptoms and macroscopic and microscopic features of the fungus (on seed and potato dextrose agar). Immersion treatment of infected seed in peppermint essential oil reduced the presence of Alternaria sp. on coriander seeds. The highest disease inhibition, 69,33%, namely 58,93% without the influence of the emulsifier, was shown by oil solution of 5%. Stagnation in inhibition was observed with a further increase in oil concentration. The results of this study show the possibility of disinfecting coriander seeds with peppermint essential oil to reduce infection with Alternaria sp.
PB  - University of Belgrade - Faculty of Agriculture
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - The possibility of coriander seed disinfection with the essential oil of peppermint [Mogućnost dezinfekcije semena korijandera etarskim uljem pitome nane]
EP  - 52
IS  - 1
SP  - 39
VL  - 66
DO  - 10.2298/JAS2101039M
ER  - 

@article{
author = "Mikić, Sara D. and Vico, Ivana M. and Duduk, Nataša D. and Marković, Tatjana Lj.",
year = "2021",
abstract = "Seed health testing aims to ensure a healthy crop and to prevent the spread of plant diseases. The need to study and apply both essential oils and plant extracts against seed diseases is growing and becoming more important. Coriander (Coriandrum sativum L.) is an annual herbaceous, aromatic, spicy and medicinal plant from the Apiaceae family, known for its seeds with a characteristic and pleasant odour. In human medicine, coriander is a part of various medicines for the treatment of digestive organs. Phytopathogenic fungi and bacteria are the most common and significant causes of coriander seed disease. The aim of this study was to assess coriander seed health and investigate the possibility of seed disinfection using peppermint (Mentha x piperita) essential oil. The health of the seeds was examined by the filter paper laboratory standard method. Different concentrations (1, 2, 5 and 10%) of peppermint essential oil were used for seed disinfection. The composition of the essential oil was determined by gas chromatographic analysis. The results of seed health analysis showed the presence of a fungus after seven days of incubation in a humid chamber at room temperature. The fungus was identified as Alternaria sp. (sect. Alternaria) based on the symptoms and macroscopic and microscopic features of the fungus (on seed and potato dextrose agar). Immersion treatment of infected seed in peppermint essential oil reduced the presence of Alternaria sp. on coriander seeds. The highest disease inhibition, 69,33%, namely 58,93% without the influence of the emulsifier, was shown by oil solution of 5%. Stagnation in inhibition was observed with a further increase in oil concentration. The results of this study show the possibility of disinfecting coriander seeds with peppermint essential oil to reduce infection with Alternaria sp.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "The possibility of coriander seed disinfection with the essential oil of peppermint [Mogućnost dezinfekcije semena korijandera etarskim uljem pitome nane]",
pages = "52-39",
number = "1",
volume = "66",
doi = "10.2298/JAS2101039M"
}

Mikić, S. D., Vico, I. M., Duduk, N. D.,& Marković, T. Lj.. (2021). The possibility of coriander seed disinfection with the essential oil of peppermint [Mogućnost dezinfekcije semena korijandera etarskim uljem pitome nane]. in Journal of Agricultural Sciences (Belgrade)
University of Belgrade - Faculty of Agriculture., 66(1), 39-52.
https://doi.org/10.2298/JAS2101039M

Mikić SD, Vico IM, Duduk ND, Marković TL. The possibility of coriander seed disinfection with the essential oil of peppermint [Mogućnost dezinfekcije semena korijandera etarskim uljem pitome nane]. in Journal of Agricultural Sciences (Belgrade). 2021;66(1):39-52.
doi:10.2298/JAS2101039M .

Mikić, Sara D., Vico, Ivana M., Duduk, Nataša D., Marković, Tatjana Lj., "The possibility of coriander seed disinfection with the essential oil of peppermint [Mogućnost dezinfekcije semena korijandera etarskim uljem pitome nane]" in Journal of Agricultural Sciences (Belgrade), 66, no. 1 (2021):39-52,
https://doi.org/10.2298/JAS2101039M . .

TY  - JOUR
AU  - Savić, Iva
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Vico, Ivana
AU  - Mladenović Drinić, Snežana
AU  - Duduk, Nataša
AU  - Stanković, Slavica
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5934
AB  - Savić I., M. Nikolić, A.Nikolić, I. Vico, S. Mladenović Drinić, N. Duduk, S. Stanković (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. - Genetika, Vol 53, No.2, 641-649. During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain
EP  - 649
IS  - 2
SP  - 641
VL  - 53
DO  - 10.2298/GENSR2102641S
ER  - 

@article{
author = "Savić, Iva and Nikolić, Milica and Nikolić, Ana and Vico, Ivana and Mladenović Drinić, Snežana and Duduk, Nataša and Stanković, Slavica",
year = "2021",
abstract = "Savić I., M. Nikolić, A.Nikolić, I. Vico, S. Mladenović Drinić, N. Duduk, S. Stanković (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. - Genetika, Vol 53, No.2, 641-649. During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain",
pages = "649-641",
number = "2",
volume = "53",
doi = "10.2298/GENSR2102641S"
}

Savić, I., Nikolić, M., Nikolić, A., Vico, I., Mladenović Drinić, S., Duduk, N.,& Stanković, S.. (2021). The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain. in Genetika
Serbian Genetics Society., 53(2), 641-649.
https://doi.org/10.2298/GENSR2102641S

Savić I, Nikolić M, Nikolić A, Vico I, Mladenović Drinić S, Duduk N, Stanković S. The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain. in Genetika. 2021;53(2):641-649.
doi:10.2298/GENSR2102641S .

Savić, Iva, Nikolić, Milica, Nikolić, Ana, Vico, Ivana, Mladenović Drinić, Snežana, Duduk, Nataša, Stanković, Slavica, "The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain" in Genetika, 53, no. 2 (2021):641-649,
https://doi.org/10.2298/GENSR2102641S . .

TY  - JOUR
AU  - Luković, Jelena
AU  - Milijašević-Marčić, Svetlana
AU  - Hatvani, Lorant
AU  - Kredics, Laszlo
AU  - Szucs, Attila
AU  - Vagvolgyi, Csaba
AU  - Duduk, Nataša
AU  - Vico, Ivana
AU  - Potocnik, Ivana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5299
AB  - Twenty-two strains of Trichoderma spp. (T. harzianum species complex [THSC], Trichoderma aggressivum f. europaeum, Trichoderma pleuroti, and Trichoderma pleuroticola) causing green mold disease on edible mushrooms (button mushroom, shiitake and oyster mushroom), collected during 2004-2018 from four countries (Serbia, North Macedonia, Croatia, and Hungary) were examined. Based on their ITS (internal transcribed spacer) sequences, strains from shiitake mushroom in Serbia were identified as members of the THSC, while in samples obtained from Serbian and North-Macedonian oyster mushroom farms THSC, T. pleuroti and T. pleuroticola were detected, which represent the first findings in the region. In fungicide susceptibility tests, all examined Trichoderma strains were found to be highly sensitive to prochloraz (ED(50)0.4 mu g mL(-1)) and considerably susceptible to metrafenone (ED50  lt  4 mu g mL(-1)). The most sensitive taxon to both fungicides was THSC from oyster mushroom. The toxicity of metrafenone was satisfying and strains from oyster mushroom showed the highest sensitivity (ED50  lt  1.43 mu g mL(-1)), while strains originating from button mushroom and shiitake displayed similar susceptibilities (ED50  lt  3.64 mu g mL(-1)). After additional in vivo trials, metrafenone might also be recommended for the control of green mold disease in mushroom farms.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
T1  - Sensitivity of Trichoderma strains from edible mushrooms to the fungicides prochloraz and metrafenone
DO  - 10.1080/03601234.2020.1838821
ER  - 

@article{
author = "Luković, Jelena and Milijašević-Marčić, Svetlana and Hatvani, Lorant and Kredics, Laszlo and Szucs, Attila and Vagvolgyi, Csaba and Duduk, Nataša and Vico, Ivana and Potocnik, Ivana",
year = "2020",
abstract = "Twenty-two strains of Trichoderma spp. (T. harzianum species complex [THSC], Trichoderma aggressivum f. europaeum, Trichoderma pleuroti, and Trichoderma pleuroticola) causing green mold disease on edible mushrooms (button mushroom, shiitake and oyster mushroom), collected during 2004-2018 from four countries (Serbia, North Macedonia, Croatia, and Hungary) were examined. Based on their ITS (internal transcribed spacer) sequences, strains from shiitake mushroom in Serbia were identified as members of the THSC, while in samples obtained from Serbian and North-Macedonian oyster mushroom farms THSC, T. pleuroti and T. pleuroticola were detected, which represent the first findings in the region. In fungicide susceptibility tests, all examined Trichoderma strains were found to be highly sensitive to prochloraz (ED(50)0.4 mu g mL(-1)) and considerably susceptible to metrafenone (ED50  lt  4 mu g mL(-1)). The most sensitive taxon to both fungicides was THSC from oyster mushroom. The toxicity of metrafenone was satisfying and strains from oyster mushroom showed the highest sensitivity (ED50  lt  1.43 mu g mL(-1)), while strains originating from button mushroom and shiitake displayed similar susceptibilities (ED50  lt  3.64 mu g mL(-1)). After additional in vivo trials, metrafenone might also be recommended for the control of green mold disease in mushroom farms.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was",
title = "Sensitivity of Trichoderma strains from edible mushrooms to the fungicides prochloraz and metrafenone",
doi = "10.1080/03601234.2020.1838821"
}

Luković, J., Milijašević-Marčić, S., Hatvani, L., Kredics, L., Szucs, A., Vagvolgyi, C., Duduk, N., Vico, I.,& Potocnik, I.. (2020). Sensitivity of Trichoderma strains from edible mushrooms to the fungicides prochloraz and metrafenone. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
Taylor & Francis Inc, Philadelphia..
https://doi.org/10.1080/03601234.2020.1838821

Luković J, Milijašević-Marčić S, Hatvani L, Kredics L, Szucs A, Vagvolgyi C, Duduk N, Vico I, Potocnik I. Sensitivity of Trichoderma strains from edible mushrooms to the fungicides prochloraz and metrafenone. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was. 2020;.
doi:10.1080/03601234.2020.1838821 .

Luković, Jelena, Milijašević-Marčić, Svetlana, Hatvani, Lorant, Kredics, Laszlo, Szucs, Attila, Vagvolgyi, Csaba, Duduk, Nataša, Vico, Ivana, Potocnik, Ivana, "Sensitivity of Trichoderma strains from edible mushrooms to the fungicides prochloraz and metrafenone" in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was (2020),
https://doi.org/10.1080/03601234.2020.1838821 . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Mitrović, Petar
AU  - Tanović, Brankica
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5227
AB  - Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
AB  - Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
T1  - Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji
EP  - 29
IS  - 1
SP  - 19
VL  - 34
DO  - 10.2298/PIF1901019V
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Mitrović, Petar and Tanović, Brankica and Vico, Ivana and Bulajić, Aleksandra",
year = "2019",
abstract = "Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet, Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji",
pages = "29-19",
number = "1",
volume = "34",
doi = "10.2298/PIF1901019V"
}

Vojvodić, M., Lazić, D., Mitrović, P., Tanović, B., Vico, I.,& Bulajić, A.. (2019). Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 19-29.
https://doi.org/10.2298/PIF1901019V

Vojvodić M, Lazić D, Mitrović P, Tanović B, Vico I, Bulajić A. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina. 2019;34(1):19-29.
doi:10.2298/PIF1901019V .

Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet" in Pesticidi i fitomedicina, 34, no. 1 (2019):19-29,
https://doi.org/10.2298/PIF1901019V . .

TY  - JOUR
AU  - Duduk, Bojan
AU  - Duduk, Nataša
AU  - Vico, Ivana
AU  - Stepanović, Jelena
AU  - Marković, Tatjana
AU  - Rekanović, Emil
AU  - Kube, Michael
AU  - Radanović, Dragoja
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4963
AB  - Floricolous downy mildews (Peronospora, oomycetes) are a small, monophyletic group of mostly inconspicuous plant pathogens that induce symptoms exclusively on flowers. Characterization of this group of pathogens, and information about their biology, is particularly sparse. The recurrent presence of a disease causing flower malformation which, in turn, leads to high production losses of the medicinal herb Matricaria chamomilla in Serbia has enabled continuous experiments focusing on the pathogen and its biology. Peronospora radii was identified as the causal agent of the disease, and morphologically and molecularly characterized. Diseased chamomile flowers showed severe malformations of the disc and ray florets, including phyllody and secondary inflorescence formation, followed by the onset of downy mildew. Phylogeny, based on internal transcribed spacer and cox2, indicates clustering of the Serbian P. radii with other P. radii from chamomile although, in cox2 analyses, they formed a separate subcluster. Evidence pointing to systemic infection was provided through histological and molecular analyses, with related experiments validating the impact of soilborne and blossom infections. This study provides new findings in the biology of P. radii on chamomile, thus enabling the reconstruction of this floricolous Peronospora species' life cycle.
PB  - Amer Phytopathological Soc, St Paul
T2  - Phytopathology
T1  - Chamomile Floricolous Downy Mildew Caused by Peronospora radii
EP  - 1907
IS  - 11
SP  - 1900
VL  - 109
DO  - 10.1094/PHYTO-04-19-0138-R
ER  - 

@article{
author = "Duduk, Bojan and Duduk, Nataša and Vico, Ivana and Stepanović, Jelena and Marković, Tatjana and Rekanović, Emil and Kube, Michael and Radanović, Dragoja",
year = "2019",
abstract = "Floricolous downy mildews (Peronospora, oomycetes) are a small, monophyletic group of mostly inconspicuous plant pathogens that induce symptoms exclusively on flowers. Characterization of this group of pathogens, and information about their biology, is particularly sparse. The recurrent presence of a disease causing flower malformation which, in turn, leads to high production losses of the medicinal herb Matricaria chamomilla in Serbia has enabled continuous experiments focusing on the pathogen and its biology. Peronospora radii was identified as the causal agent of the disease, and morphologically and molecularly characterized. Diseased chamomile flowers showed severe malformations of the disc and ray florets, including phyllody and secondary inflorescence formation, followed by the onset of downy mildew. Phylogeny, based on internal transcribed spacer and cox2, indicates clustering of the Serbian P. radii with other P. radii from chamomile although, in cox2 analyses, they formed a separate subcluster. Evidence pointing to systemic infection was provided through histological and molecular analyses, with related experiments validating the impact of soilborne and blossom infections. This study provides new findings in the biology of P. radii on chamomile, thus enabling the reconstruction of this floricolous Peronospora species' life cycle.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Chamomile Floricolous Downy Mildew Caused by Peronospora radii",
pages = "1907-1900",
number = "11",
volume = "109",
doi = "10.1094/PHYTO-04-19-0138-R"
}

Duduk, B., Duduk, N., Vico, I., Stepanović, J., Marković, T., Rekanović, E., Kube, M.,& Radanović, D.. (2019). Chamomile Floricolous Downy Mildew Caused by Peronospora radii. in Phytopathology
Amer Phytopathological Soc, St Paul., 109(11), 1900-1907.
https://doi.org/10.1094/PHYTO-04-19-0138-R

Duduk B, Duduk N, Vico I, Stepanović J, Marković T, Rekanović E, Kube M, Radanović D. Chamomile Floricolous Downy Mildew Caused by Peronospora radii. in Phytopathology. 2019;109(11):1900-1907.
doi:10.1094/PHYTO-04-19-0138-R .

Duduk, Bojan, Duduk, Nataša, Vico, Ivana, Stepanović, Jelena, Marković, Tatjana, Rekanović, Emil, Kube, Michael, Radanović, Dragoja, "Chamomile Floricolous Downy Mildew Caused by Peronospora radii" in Phytopathology, 109, no. 11 (2019):1900-1907,
https://doi.org/10.1094/PHYTO-04-19-0138-R . .

TY  - JOUR
AU  - Žebeljan, Aleksandra
AU  - Vico, Ivana
AU  - Duduk, Nataša
AU  - Ziberna, Bojana
AU  - Krajnc, Andreja Urbanek
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5070
AB  - Penicillium expansum-infected apple fruit resulted in a disrupted balance of primary metabolites and impacted the ascorbate-glutathione cycle. The initial decline in fructose, malic acid, shikimic acid and total ascorbate content (6 hpi) was followed by a significant increase in malic acid (24 hpi) and total glutathione (3 dpi). Significant increase in sucrose was accompanied by a decline in glucose and ascorbate content (5 dpi). Total glutathione and shikimic acid gradually deteriorated with symptom development. A negative correlation was found between sucrose and ascorbate-glutathione pool. Other correlations were strong amongst other metabolites and lesion size indicating that they may be involved or important for apple fruit decay.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Physiological and Molecular Plant Pathology
T1  - Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions
EP  - 174
SP  - 166
VL  - 106
DO  - 10.1016/j.pmpp.2019.02.001
ER  - 

@article{
author = "Žebeljan, Aleksandra and Vico, Ivana and Duduk, Nataša and Ziberna, Bojana and Krajnc, Andreja Urbanek",
year = "2019",
abstract = "Penicillium expansum-infected apple fruit resulted in a disrupted balance of primary metabolites and impacted the ascorbate-glutathione cycle. The initial decline in fructose, malic acid, shikimic acid and total ascorbate content (6 hpi) was followed by a significant increase in malic acid (24 hpi) and total glutathione (3 dpi). Significant increase in sucrose was accompanied by a decline in glucose and ascorbate content (5 dpi). Total glutathione and shikimic acid gradually deteriorated with symptom development. A negative correlation was found between sucrose and ascorbate-glutathione pool. Other correlations were strong amongst other metabolites and lesion size indicating that they may be involved or important for apple fruit decay.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Physiological and Molecular Plant Pathology",
title = "Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions",
pages = "174-166",
volume = "106",
doi = "10.1016/j.pmpp.2019.02.001"
}

Žebeljan, A., Vico, I., Duduk, N., Ziberna, B.,& Krajnc, A. U.. (2019). Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions. in Physiological and Molecular Plant Pathology
Academic Press Ltd- Elsevier Science Ltd, London., 106, 166-174.
https://doi.org/10.1016/j.pmpp.2019.02.001

Žebeljan A, Vico I, Duduk N, Ziberna B, Krajnc AU. Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions. in Physiological and Molecular Plant Pathology. 2019;106:166-174.
doi:10.1016/j.pmpp.2019.02.001 .

Žebeljan, Aleksandra, Vico, Ivana, Duduk, Nataša, Ziberna, Bojana, Krajnc, Andreja Urbanek, "Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions" in Physiological and Molecular Plant Pathology, 106 (2019):166-174,
https://doi.org/10.1016/j.pmpp.2019.02.001 . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mihajlović, Milica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4898
AB  - Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
AB  - Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
T1  - Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji
EP  - 107
IS  - 2
SP  - 97
VL  - 33
DO  - 10.2298/PIF1802097V
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mihajlović, Milica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2018",
abstract = "Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia, Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji",
pages = "107-97",
number = "2",
volume = "33",
doi = "10.2298/PIF1802097V"
}

Vojvodić, M., Tanović, B., Mihajlović, M., Mitrović, P., Vico, I.,& Bulajić, A.. (2018). Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(2), 97-107.
https://doi.org/10.2298/PIF1802097V

Vojvodić M, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina. 2018;33(2):97-107.
doi:10.2298/PIF1802097V .

Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia" in Pesticidi i fitomedicina, 33, no. 2 (2018):97-107,
https://doi.org/10.2298/PIF1802097V . .

TY  - JOUR
AU  - Vasić, Miljan
AU  - Vico, Ivana
AU  - Jurick, Wayne M., II
AU  - Duduk, Nataša
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4741
AB  - Brown rot, caused by Monilinia spp., is an economically important pre and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia
EP  - 369
IS  - 2
SP  - 359
VL  - 102
DO  - 10.1094/PDIS-06-17-0867-RE
ER  - 

@article{
author = "Vasić, Miljan and Vico, Ivana and Jurick, Wayne M., II and Duduk, Nataša",
year = "2018",
abstract = "Brown rot, caused by Monilinia spp., is an economically important pre and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia",
pages = "369-359",
number = "2",
volume = "102",
doi = "10.1094/PDIS-06-17-0867-RE"
}

Vasić, M., Vico, I., Jurick, W. M., I.,& Duduk, N.. (2018). Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 102(2), 359-369.
https://doi.org/10.1094/PDIS-06-17-0867-RE

Vasić M, Vico I, Jurick WMI, Duduk N. Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia. in Plant Disease. 2018;102(2):359-369.
doi:10.1094/PDIS-06-17-0867-RE .

Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., II, Duduk, Nataša, "Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia" in Plant Disease, 102, no. 2 (2018):359-369,
https://doi.org/10.1094/PDIS-06-17-0867-RE . .

TY  - JOUR
AU  - Luković, Jelena
AU  - Stepanović, Miloš
AU  - Todorović, Biljana
AU  - Milijašević-Marčić, Svetlana
AU  - Duduk, Nataša
AU  - Vico, Ivana
AU  - Potočnik, Ivana
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4889
AB  - Antifungal activity of two essential oils, cinnamon (Cinnamomum verum J. Presl) and clove (Syzygium aromaticum (L.) Merrill & Perry), was evaluated against Cladobotryum dendroides (Bull.) W. Gams & Hooz, and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, the causal agents of cobweb and dry bubble disease of cultivated mushroom. Inhibitory and fungicidal activity of the selected essential oils was assayed using three methods: microdilution, macrodilution fumigant and macrodilution contact method. Comparing all three methods, clove essential oil showed stronger activity than cinnamon against both fungi, having minimum inhibitory concentration (MIC) at the lowest concentrations tested (1.56, 0.02 and 0.1 µl ml-1, respectively). However, cinnamon oil was more toxic to L. fungicola var. fungicola then to C. dendroides in all three methods. Both oils exhibited stronger antifungal effects when used in the macrodilution fumigant than in contact method. The results showed that both cinnamon and clove essential oils have the potential for further in vivo experiments against L. fungicola var. fungicola and C. dendroides and indicated a possible use of these oils in integrated disease management in mushrooms.
AB  - Ispitana je antifungalna aktivnost etarskih ulja cimeta i karanfilića prema Cladobotryum dendroides (Bull.) W. Gams & Hooz, i Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, prouzrokovačima paučinaste plesni i suve truleži šampinjona. Antifungalna aktivnost odabranih etarskih ulja testirana je primenom tri različite metode: mikrodilucione, makrodilucione fumigantne i makrodilucione kontaktne metode. Etarsko ulje karanfilića je ispoljilo jači efekat od ulja cimeta na obe gljive primenom sve tri metode, sa minimalnim inhibitornim koncentrecijama pri najnižim testiranim koncentracijama (1,56, 0,02 i 0,1 µl ml-1). Ulje cimeta je pokazalo veću toksičnost prema L. fungicola var. fungicola nego prema C. dendroides. Ispitivana etarska ulja ispoljila su jače antifungalno dejstvo primenjena fumigantno nego kontaktno u makrodilucionoj metodi. Antifungalno delovanje etarskih ulja cimeta i karanfilića in vitro, ukazuje na potencijal za dalja ispitivanja njihove efikasnosti in vivo, kao i mogućnosti uključivanja ovih etarskih ulja u integralnu zaštitu jestivih gljiva od bolesti prouzrokovanih mikopatogenim gljivama L. fungicola var. fungicola i C. dendroides.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk
T1  - Antifungalna aktivnost etarskih ulja cimeta i karanfilića prema patogenima šampinjona - Cladobotryum dendroides (Bull.) W. Gams & Hooz i Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk
EP  - 26
IS  - 1
SP  - 19
VL  - 33
DO  - 10.2298/PIF1801019L
ER  - 

@article{
author = "Luković, Jelena and Stepanović, Miloš and Todorović, Biljana and Milijašević-Marčić, Svetlana and Duduk, Nataša and Vico, Ivana and Potočnik, Ivana",
year = "2018",
abstract = "Antifungal activity of two essential oils, cinnamon (Cinnamomum verum J. Presl) and clove (Syzygium aromaticum (L.) Merrill & Perry), was evaluated against Cladobotryum dendroides (Bull.) W. Gams & Hooz, and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, the causal agents of cobweb and dry bubble disease of cultivated mushroom. Inhibitory and fungicidal activity of the selected essential oils was assayed using three methods: microdilution, macrodilution fumigant and macrodilution contact method. Comparing all three methods, clove essential oil showed stronger activity than cinnamon against both fungi, having minimum inhibitory concentration (MIC) at the lowest concentrations tested (1.56, 0.02 and 0.1 µl ml-1, respectively). However, cinnamon oil was more toxic to L. fungicola var. fungicola then to C. dendroides in all three methods. Both oils exhibited stronger antifungal effects when used in the macrodilution fumigant than in contact method. The results showed that both cinnamon and clove essential oils have the potential for further in vivo experiments against L. fungicola var. fungicola and C. dendroides and indicated a possible use of these oils in integrated disease management in mushrooms., Ispitana je antifungalna aktivnost etarskih ulja cimeta i karanfilića prema Cladobotryum dendroides (Bull.) W. Gams & Hooz, i Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, prouzrokovačima paučinaste plesni i suve truleži šampinjona. Antifungalna aktivnost odabranih etarskih ulja testirana je primenom tri različite metode: mikrodilucione, makrodilucione fumigantne i makrodilucione kontaktne metode. Etarsko ulje karanfilića je ispoljilo jači efekat od ulja cimeta na obe gljive primenom sve tri metode, sa minimalnim inhibitornim koncentrecijama pri najnižim testiranim koncentracijama (1,56, 0,02 i 0,1 µl ml-1). Ulje cimeta je pokazalo veću toksičnost prema L. fungicola var. fungicola nego prema C. dendroides. Ispitivana etarska ulja ispoljila su jače antifungalno dejstvo primenjena fumigantno nego kontaktno u makrodilucionoj metodi. Antifungalno delovanje etarskih ulja cimeta i karanfilića in vitro, ukazuje na potencijal za dalja ispitivanja njihove efikasnosti in vivo, kao i mogućnosti uključivanja ovih etarskih ulja u integralnu zaštitu jestivih gljiva od bolesti prouzrokovanih mikopatogenim gljivama L. fungicola var. fungicola i C. dendroides.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, Antifungalna aktivnost etarskih ulja cimeta i karanfilića prema patogenima šampinjona - Cladobotryum dendroides (Bull.) W. Gams & Hooz i Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk",
pages = "26-19",
number = "1",
volume = "33",
doi = "10.2298/PIF1801019L"
}

Luković, J., Stepanović, M., Todorović, B., Milijašević-Marčić, S., Duduk, N., Vico, I.,& Potočnik, I.. (2018). Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(1), 19-26.
https://doi.org/10.2298/PIF1801019L

Luković J, Stepanović M, Todorović B, Milijašević-Marčić S, Duduk N, Vico I, Potočnik I. Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk. in Pesticidi i fitomedicina. 2018;33(1):19-26.
doi:10.2298/PIF1801019L .

Luković, Jelena, Stepanović, Miloš, Todorović, Biljana, Milijašević-Marčić, Svetlana, Duduk, Nataša, Vico, Ivana, Potočnik, Ivana, "Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk" in Pesticidi i fitomedicina, 33, no. 1 (2018):19-26,
https://doi.org/10.2298/PIF1801019L . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Vasić, Miljan M.
AU  - Vučković, Nina R.
AU  - Žebeljan, Aleksandra
AU  - Vico, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4574
AB  - Monilinia spp. are economically important pathogens of pome and stone fruits. Four Monilinia species are present in Serbia - Monilinia fructigena, M. laxa, M. fructicola and Monilia polystroma. As detection and identification of Monilinia species are complex, the aim of this research was to evaluate species-specific primers in PCR in order to standardize fast and reliable molecular methods for differentiation between the four Monilinia species. Isolates of M. fructigena, M. laxa, M. fructicola and M. polystroma from apple fruit and referent isolates from Italy and Japan were used for testing. Specific molecular detection of M. laxa was obtained using ITS1Mlx/ITS4Mlx and Ml-Mfg-F2/Ml-Mfc-R1 primer pairs, and M. fructicola using ITS1Mfcl/ITS4Mfcl and Mfc-F1/Mfc-R1 primer pairs. ITS1Mfgn/ITS4Mfgn and ITS1/Mfg-R2 primer pairs, described as M. fructigena species-specific, amplified M. fructigena and M. polystroma, as well. Specific detection of these two species as well as of all four tested Monilinia species was obtained using the reverse primer MO368-5 with forward primers MO368-8R, Laxa-R2 and MO368-10R in separate or in Multiplex PCR reactions.
AB  - Vrste roda Monilinia su ekonomski znač ajni patogeni jabučastih i koštičavih vrsta voćaka. U Srbiji su prisutne četiri vrste ovog roda: M. fructigena, M. laxa, M. fructicola i Monilia polystroma. Detekcija i identifikacija vrsta roda Monilinia je složena, tako da je cilj ovog rada bio da se ispita i standardizuje brza i efikasna metoda molekularne detekcije korišćenjem različitih prajmera u PCR metodi i da se utvrdi njihova specifičnost i mogućnost korišćenja u identifikaciji Monilinia spp. U radu su korišćeni izolati M. fructigena, M. laxa, M. fructicola i M. polystroma poreklom iz plodova jabuke prikupljenih u Srbiji, kao i referentni izolati iz Italije i Japana. Specifična molekularna detekcija M. laxa postignuta je korišćenjem prajmera ITS1Mlx/ITS4Mlx i Ml-Mfg -F2/Ml-Mfc-R1, a vrste M. fructicola korišćenjem prajmera ITS1Mfcl/ITS4Mfcl i Mfc-F1/Mfc-R1. Prajmeri ITS1Mfgn/ITS4Mfgn i ITS1/Mfg -R2, koji su u literaturi opisani kao specifični za M. fructigena, pored izolata M. fructigena amplifikovali su i DNK izolata M. polystroma. Za razdvajanje ove dve vrste, kao i za razlikovanje sve četiri ispitivane vrste roda Monilinia, najpogodniji su uzvodni prajmer MO368-5 u kombinaciji sa nizvodnim prajmerima MO368-8R, Laxa-R2 i MO368-10R u direktnoj ili Multiplex PCR metodi.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Suitability of different primers for specific molecular detection of Monilinia spp.
T1  - Pogodnost različitih prajmera za specifičnu molekularnu detekciju Monilinia spp.
EP  - 177
IS  - 2
SP  - 167
VL  - 62
DO  - 10.2298/JAS1702167D
ER  - 

@article{
author = "Duduk, Nataša and Vasić, Miljan M. and Vučković, Nina R. and Žebeljan, Aleksandra and Vico, Ivana",
year = "2017",
abstract = "Monilinia spp. are economically important pathogens of pome and stone fruits. Four Monilinia species are present in Serbia - Monilinia fructigena, M. laxa, M. fructicola and Monilia polystroma. As detection and identification of Monilinia species are complex, the aim of this research was to evaluate species-specific primers in PCR in order to standardize fast and reliable molecular methods for differentiation between the four Monilinia species. Isolates of M. fructigena, M. laxa, M. fructicola and M. polystroma from apple fruit and referent isolates from Italy and Japan were used for testing. Specific molecular detection of M. laxa was obtained using ITS1Mlx/ITS4Mlx and Ml-Mfg-F2/Ml-Mfc-R1 primer pairs, and M. fructicola using ITS1Mfcl/ITS4Mfcl and Mfc-F1/Mfc-R1 primer pairs. ITS1Mfgn/ITS4Mfgn and ITS1/Mfg-R2 primer pairs, described as M. fructigena species-specific, amplified M. fructigena and M. polystroma, as well. Specific detection of these two species as well as of all four tested Monilinia species was obtained using the reverse primer MO368-5 with forward primers MO368-8R, Laxa-R2 and MO368-10R in separate or in Multiplex PCR reactions., Vrste roda Monilinia su ekonomski znač ajni patogeni jabučastih i koštičavih vrsta voćaka. U Srbiji su prisutne četiri vrste ovog roda: M. fructigena, M. laxa, M. fructicola i Monilia polystroma. Detekcija i identifikacija vrsta roda Monilinia je složena, tako da je cilj ovog rada bio da se ispita i standardizuje brza i efikasna metoda molekularne detekcije korišćenjem različitih prajmera u PCR metodi i da se utvrdi njihova specifičnost i mogućnost korišćenja u identifikaciji Monilinia spp. U radu su korišćeni izolati M. fructigena, M. laxa, M. fructicola i M. polystroma poreklom iz plodova jabuke prikupljenih u Srbiji, kao i referentni izolati iz Italije i Japana. Specifična molekularna detekcija M. laxa postignuta je korišćenjem prajmera ITS1Mlx/ITS4Mlx i Ml-Mfg -F2/Ml-Mfc-R1, a vrste M. fructicola korišćenjem prajmera ITS1Mfcl/ITS4Mfcl i Mfc-F1/Mfc-R1. Prajmeri ITS1Mfgn/ITS4Mfgn i ITS1/Mfg -R2, koji su u literaturi opisani kao specifični za M. fructigena, pored izolata M. fructigena amplifikovali su i DNK izolata M. polystroma. Za razdvajanje ove dve vrste, kao i za razlikovanje sve četiri ispitivane vrste roda Monilinia, najpogodniji su uzvodni prajmer MO368-5 u kombinaciji sa nizvodnim prajmerima MO368-8R, Laxa-R2 i MO368-10R u direktnoj ili Multiplex PCR metodi.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Suitability of different primers for specific molecular detection of Monilinia spp., Pogodnost različitih prajmera za specifičnu molekularnu detekciju Monilinia spp.",
pages = "177-167",
number = "2",
volume = "62",
doi = "10.2298/JAS1702167D"
}

Duduk, N., Vasić, M. M., Vučković, N. R., Žebeljan, A.,& Vico, I.. (2017). Suitability of different primers for specific molecular detection of Monilinia spp.. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 62(2), 167-177.
https://doi.org/10.2298/JAS1702167D

Duduk N, Vasić MM, Vučković NR, Žebeljan A, Vico I. Suitability of different primers for specific molecular detection of Monilinia spp.. in Journal of Agricultural Sciences (Belgrade). 2017;62(2):167-177.
doi:10.2298/JAS1702167D .

Duduk, Nataša, Vasić, Miljan M., Vučković, Nina R., Žebeljan, Aleksandra, Vico, Ivana, "Suitability of different primers for specific molecular detection of Monilinia spp." in Journal of Agricultural Sciences (Belgrade), 62, no. 2 (2017):167-177,
https://doi.org/10.2298/JAS1702167D . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Lazarević, Marina
AU  - Žebeljan, Aleksandra
AU  - Vasić, Miljan
AU  - Vico, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4452
AB  - Onion bulbs (Allium cepa L.) can be stored for up to 12months to ensure their availability all year, but one of the limiting factors is decay caused by blue mould fungi. Postharvest development of blue mould is not only economically important, but also poses a health hazard due to mycotoxin contamination. Onion bulbs of different yellow and red cultivars with blue mould symptoms were collected from three storage facilities in Serbia. Pathogenic isolates were identified and characterized based on their molecular (partial -tubulin gene), morphological (micro- and macromorphology, and colony growth) and biochemical properties (analyses of indole metabolites via Ehrlich test). Three Penicillium species were identified: P.polonicum, P.glabrum and P.expansum. Virulence on inoculated onion bulbs varied significantly within isolates of P.glabrum, but the most virulent isolate of P.polonicum and P.glabrum did not differ, nor did they differ from an isolate of P.expansum. Variability in virulence of individual P.glabrum isolates corresponded to differences in their molecular and macromorphological characters. P.glabrum and P.expansum were identified for the first time as causal agents of onion bulb decay in Serbia. Data from this investigation provide insight into diversity of economically important and possibly toxigenic blue mould fungi which brings attention to their presence in storage facilities and therefore the necessity of the application of prevention measures.
PB  - Wiley, Hoboken
T2  - Journal of Phytopathology
T1  - Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum
EP  - 669
IS  - 10
SP  - 662
VL  - 165
DO  - 10.1111/jph.12605
ER  - 

@article{
author = "Duduk, Nataša and Lazarević, Marina and Žebeljan, Aleksandra and Vasić, Miljan and Vico, Ivana",
year = "2017",
abstract = "Onion bulbs (Allium cepa L.) can be stored for up to 12months to ensure their availability all year, but one of the limiting factors is decay caused by blue mould fungi. Postharvest development of blue mould is not only economically important, but also poses a health hazard due to mycotoxin contamination. Onion bulbs of different yellow and red cultivars with blue mould symptoms were collected from three storage facilities in Serbia. Pathogenic isolates were identified and characterized based on their molecular (partial -tubulin gene), morphological (micro- and macromorphology, and colony growth) and biochemical properties (analyses of indole metabolites via Ehrlich test). Three Penicillium species were identified: P.polonicum, P.glabrum and P.expansum. Virulence on inoculated onion bulbs varied significantly within isolates of P.glabrum, but the most virulent isolate of P.polonicum and P.glabrum did not differ, nor did they differ from an isolate of P.expansum. Variability in virulence of individual P.glabrum isolates corresponded to differences in their molecular and macromorphological characters. P.glabrum and P.expansum were identified for the first time as causal agents of onion bulb decay in Serbia. Data from this investigation provide insight into diversity of economically important and possibly toxigenic blue mould fungi which brings attention to their presence in storage facilities and therefore the necessity of the application of prevention measures.",
publisher = "Wiley, Hoboken",
journal = "Journal of Phytopathology",
title = "Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum",
pages = "669-662",
number = "10",
volume = "165",
doi = "10.1111/jph.12605"
}

Duduk, N., Lazarević, M., Žebeljan, A., Vasić, M.,& Vico, I.. (2017). Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum. in Journal of Phytopathology
Wiley, Hoboken., 165(10), 662-669.
https://doi.org/10.1111/jph.12605

Duduk N, Lazarević M, Žebeljan A, Vasić M, Vico I. Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum. in Journal of Phytopathology. 2017;165(10):662-669.
doi:10.1111/jph.12605 .

Duduk, Nataša, Lazarević, Marina, Žebeljan, Aleksandra, Vasić, Miljan, Vico, Ivana, "Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum" in Journal of Phytopathology, 165, no. 10 (2017):662-669,
https://doi.org/10.1111/jph.12605 . .

TY  - JOUR
AU  - Vico, Ivana
AU  - Žebeljan, Aleksandra
AU  - Vucković, N.
AU  - Vasić, M.
AU  - Duduk, Nataša
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4496
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia
EP  - 1823
IS  - 10
SP  - 1823
VL  - 101
DO  - 10.1094/PDIS-04-17-0484-PDN
ER  - 

@article{
author = "Vico, Ivana and Žebeljan, Aleksandra and Vucković, N. and Vasić, M. and Duduk, Nataša",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia",
pages = "1823-1823",
number = "10",
volume = "101",
doi = "10.1094/PDIS-04-17-0484-PDN"
}

Vico, I., Žebeljan, A., Vucković, N., Vasić, M.,& Duduk, N.. (2017). First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(10), 1823-1823.
https://doi.org/10.1094/PDIS-04-17-0484-PDN

Vico I, Žebeljan A, Vucković N, Vasić M, Duduk N. First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia. in Plant Disease. 2017;101(10):1823-1823.
doi:10.1094/PDIS-04-17-0484-PDN .

Vico, Ivana, Žebeljan, Aleksandra, Vucković, N., Vasić, M., Duduk, Nataša, "First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia" in Plant Disease, 101, no. 10 (2017):1823-1823,
https://doi.org/10.1094/PDIS-04-17-0484-PDN . .

TY  - CONF
AU  - Jurick, Wayne M., II
AU  - Macarisin, O.
AU  - Gaskins, Verneta L.
AU  - Vico, Ivana
AU  - Yu, J.
AU  - Peter, K.A.
AU  - Janisiewicz, Wojciech
AU  - Cox, K.D.
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4443
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management
EP  - 63
IS  - 12
SP  - 63
VL  - 107
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4443
ER  - 

@conference{
author = "Jurick, Wayne M., II and Macarisin, O. and Gaskins, Verneta L. and Vico, Ivana and Yu, J. and Peter, K.A. and Janisiewicz, Wojciech and Cox, K.D.",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management",
pages = "63-63",
number = "12",
volume = "107",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4443"
}

Jurick, W. M., I., Macarisin, O., Gaskins, V. L., Vico, I., Yu, J., Peter, K.A., Janisiewicz, W.,& Cox, K.D.. (2017). Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management. in Phytopathology
Amer Phytopathological Soc, St Paul., 107(12), 63-63.
https://hdl.handle.net/21.15107/rcub_agrospace_4443

Jurick WMI, Macarisin O, Gaskins VL, Vico I, Yu J, Peter K, Janisiewicz W, Cox K. Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management. in Phytopathology. 2017;107(12):63-63.
https://hdl.handle.net/21.15107/rcub_agrospace_4443 .

Jurick, Wayne M., II, Macarisin, O., Gaskins, Verneta L., Vico, Ivana, Yu, J., Peter, K.A., Janisiewicz, Wojciech, Cox, K.D., "Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management" in Phytopathology, 107, no. 12 (2017):63-63,
https://hdl.handle.net/21.15107/rcub_agrospace_4443 .

TY  - JOUR
AU  - Vasić, M.
AU  - Duduk, Nataša
AU  - Vico, Ivana
AU  - Rančić, Dragana
AU  - Pajić, V.
AU  - Backhouse, D.
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4135
AB  - In this study, we compared cultural, morphological, pathogenic and biological features of Serbian isolates of Monilia polystroma and Monilinia fructigena from apple fruit. Isolates of M. polystroma formed characteristic stromatal plates throughout the cultures unlike M. fructigena, while mycelial growth rates were not distinguishable features between species. Conidia of M. fructigena were larger than M. polystroma, and both species produced slightly larger conidia on apple fruit than on V8 medium. The existence and morphology of the microconidial state of M. polystroma was described for the first time using light and SEM microscopy. Microconidia were globose (2.19-3.44 mu m), similar to M. fructigena. Digestion of polymerase chain reaction (PCR) product of ribosomal ITS1-5.8S-ITS2 region with restriction enzyme HhaI provided a reliable method for specific detection of M. polystroma. In a pathogenicity test, both species produced larger lesions on cv. Golden Delicious, than on cvs. Idared and Granny Smith. On cv. Golden Delicious, the mean lesion size was not significatly different between species, while on cvs. Idared and Granny Smith M. fructigena tended to produce slightly larger lesions compared to M. polystroma. On artificially inoculated apple fruit after 1 and 4 months of incubation, M. polystroma, unlike M. fructigena, was capable of forming thicker and more compact stromata with complex morphology, which consisted of densely interwoven medullary hyphae covered with a discontinuous layer of melanized hyphal rind cells. The dynamics of stromata formation beneath the host's cuticle also showed differences between two species, where M. polystroma formed this layer after 1 month, while M. fructigena after 4 months of incubation.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology
EP  - 30
IS  - 1
SP  - 15
VL  - 144
DO  - 10.1007/s10658-015-0740-6
ER  - 

@article{
author = "Vasić, M. and Duduk, Nataša and Vico, Ivana and Rančić, Dragana and Pajić, V. and Backhouse, D.",
year = "2016",
abstract = "In this study, we compared cultural, morphological, pathogenic and biological features of Serbian isolates of Monilia polystroma and Monilinia fructigena from apple fruit. Isolates of M. polystroma formed characteristic stromatal plates throughout the cultures unlike M. fructigena, while mycelial growth rates were not distinguishable features between species. Conidia of M. fructigena were larger than M. polystroma, and both species produced slightly larger conidia on apple fruit than on V8 medium. The existence and morphology of the microconidial state of M. polystroma was described for the first time using light and SEM microscopy. Microconidia were globose (2.19-3.44 mu m), similar to M. fructigena. Digestion of polymerase chain reaction (PCR) product of ribosomal ITS1-5.8S-ITS2 region with restriction enzyme HhaI provided a reliable method for specific detection of M. polystroma. In a pathogenicity test, both species produced larger lesions on cv. Golden Delicious, than on cvs. Idared and Granny Smith. On cv. Golden Delicious, the mean lesion size was not significatly different between species, while on cvs. Idared and Granny Smith M. fructigena tended to produce slightly larger lesions compared to M. polystroma. On artificially inoculated apple fruit after 1 and 4 months of incubation, M. polystroma, unlike M. fructigena, was capable of forming thicker and more compact stromata with complex morphology, which consisted of densely interwoven medullary hyphae covered with a discontinuous layer of melanized hyphal rind cells. The dynamics of stromata formation beneath the host's cuticle also showed differences between two species, where M. polystroma formed this layer after 1 month, while M. fructigena after 4 months of incubation.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology",
pages = "30-15",
number = "1",
volume = "144",
doi = "10.1007/s10658-015-0740-6"
}

Vasić, M., Duduk, N., Vico, I., Rančić, D., Pajić, V.,& Backhouse, D.. (2016). Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology. in European Journal of Plant Pathology
Springer, Dordrecht., 144(1), 15-30.
https://doi.org/10.1007/s10658-015-0740-6

Vasić M, Duduk N, Vico I, Rančić D, Pajić V, Backhouse D. Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology. in European Journal of Plant Pathology. 2016;144(1):15-30.
doi:10.1007/s10658-015-0740-6 .

Vasić, M., Duduk, Nataša, Vico, Ivana, Rančić, Dragana, Pajić, V., Backhouse, D., "Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology" in European Journal of Plant Pathology, 144, no. 1 (2016):15-30,
https://doi.org/10.1007/s10658-015-0740-6 . .

TY  - CONF
AU  - Vico, Ivana
AU  - Duduk, Nataša
AU  - Vasić, M.
AU  - Žebeljan, Aleksandra
AU  - Radivojević, D.
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4141
AB  - Bull's eye rot caused by Neofabraea spp. is an important postharvest disease of apple fruit worldwide. Four species of the genus are known to cause this disease among which Neofabraea alba is the main pathogen causing bull's eye rot in continental Europe. Typical symptoms of the disease were observed in a local market in Belgrade, Serbia in March 2015. Circular lesions, slightly sunken light brown to dark brown with a lighter brown to tan center and a darker outer ring were present on 'Golden Delicious' apples. Decayed tissue was firm. The aim of this study was to identify the causal agent of bull's eye rot of apple fruit based on morphological and molecular characteristics of the pathogen. Two fungal isolates were obtained using standard laboratory procedure and their pathogenicity was tested by wound inoculation of healthy 'Idared' apple fruit. Seven days post inoculation lesions up to 23 cm in diameter developed on inoculated fruit while control fruit remained healthy. The isolates were preliminary identified based on colony morphology. They formed round, slow growing colonies on potato dextrose agar (PDA) with initially white mycelium which turned pinkish-brown with time. Cylindrical to curved-fusiform macroconidia were formed. Microconidia were not observed. Species level identification was completed by sequence analysis of the partial beta-tubulin gene. MegaBLAST analysis of the obtained nucleotide sequences (622 nt) revealed 100% and 99% similarity with several Neofabraea alba sequences deposited in GenBank. Based on morphological characteristics and the partial sequence analysis of p-tubulin gene, Neofabraea alba (Guthrie) Verkley was identified as the causal agent of bull's eye rot of apple fruit in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - Bull's eye rot of apple fruit caused by Neofabraea alba
EP  - 737
SP  - 733
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.125
ER  - 

@conference{
author = "Vico, Ivana and Duduk, Nataša and Vasić, M. and Žebeljan, Aleksandra and Radivojević, D.",
year = "2016",
abstract = "Bull's eye rot caused by Neofabraea spp. is an important postharvest disease of apple fruit worldwide. Four species of the genus are known to cause this disease among which Neofabraea alba is the main pathogen causing bull's eye rot in continental Europe. Typical symptoms of the disease were observed in a local market in Belgrade, Serbia in March 2015. Circular lesions, slightly sunken light brown to dark brown with a lighter brown to tan center and a darker outer ring were present on 'Golden Delicious' apples. Decayed tissue was firm. The aim of this study was to identify the causal agent of bull's eye rot of apple fruit based on morphological and molecular characteristics of the pathogen. Two fungal isolates were obtained using standard laboratory procedure and their pathogenicity was tested by wound inoculation of healthy 'Idared' apple fruit. Seven days post inoculation lesions up to 23 cm in diameter developed on inoculated fruit while control fruit remained healthy. The isolates were preliminary identified based on colony morphology. They formed round, slow growing colonies on potato dextrose agar (PDA) with initially white mycelium which turned pinkish-brown with time. Cylindrical to curved-fusiform macroconidia were formed. Microconidia were not observed. Species level identification was completed by sequence analysis of the partial beta-tubulin gene. MegaBLAST analysis of the obtained nucleotide sequences (622 nt) revealed 100% and 99% similarity with several Neofabraea alba sequences deposited in GenBank. Based on morphological characteristics and the partial sequence analysis of p-tubulin gene, Neofabraea alba (Guthrie) Verkley was identified as the causal agent of bull's eye rot of apple fruit in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "Bull's eye rot of apple fruit caused by Neofabraea alba",
pages = "737-733",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.125"
}

Vico, I., Duduk, N., Vasić, M., Žebeljan, A.,& Radivojević, D.. (2016). Bull's eye rot of apple fruit caused by Neofabraea alba. in III Balkan Symposium on Fruit Growing
Int Soc Horticultural Science, Leuven 1., 1139, 733-737.
https://doi.org/10.17660/ActaHortic.2016.1139.125

Vico I, Duduk N, Vasić M, Žebeljan A, Radivojević D. Bull's eye rot of apple fruit caused by Neofabraea alba. in III Balkan Symposium on Fruit Growing. 2016;1139:733-737.
doi:10.17660/ActaHortic.2016.1139.125 .

Vico, Ivana, Duduk, Nataša, Vasić, M., Žebeljan, Aleksandra, Radivojević, D., "Bull's eye rot of apple fruit caused by Neofabraea alba" in III Balkan Symposium on Fruit Growing, 1139 (2016):733-737,
https://doi.org/10.17660/ActaHortic.2016.1139.125 . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Marković, Tatjana
AU  - Vasić, Miljan
AU  - Duduk, Bojan
AU  - Vico, Ivana
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3825
AB  - The antifungal effects of thyme, cinnamon bark and clove bud essential oils (EOs) were investigated in vitro on Colletotrichum acutatum mycelial growth, conidial germination, appressoria formation, and in vivo on strawberry fruit disease incidence. All tested EOs, incorporated in potato-dextrose agar, inhibitedC. acutatum mycelial growth, and had a fungistatic effect at concentration 667 mu l/l of medium. Volatiles of cinnamon bark, thyme and clove bud EOs completely prevented conidial germination at the lowest concentrations of 1.53, 15.3 and 76.5 mu l/l of air, respectively, and disabled appressoria formation at concentration of 1.53 mu l/l of air. On inoculated strawberry fruit, thyme and cinnamon bark EO volatiles reduced anthracnose incidence at concentrations above 15.3 and 76.5 mu l/l of air, respectively. GC-FID and GC-MS analysis showed that major components of thyme EO were p-cymene, thymol, alpha-terpineol, carvacrol; cinnamon bark EO: trans- cinnameldehyde, trans-cinnamyl acetate; clove bud EO: eugenol and beta-caryophyllene. Our results suggest thatvolatiles of thyme and cinnamon bark EOs are effective against C. acutatum both in vitro and in vivo.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose
EP  - 537
IS  - 3
SP  - 529
VL  - 18
DO  - 10.1080/0972060X.2015.1004120
ER  - 

@article{
author = "Duduk, Nataša and Marković, Tatjana and Vasić, Miljan and Duduk, Bojan and Vico, Ivana and Obradović, Aleksa",
year = "2015",
abstract = "The antifungal effects of thyme, cinnamon bark and clove bud essential oils (EOs) were investigated in vitro on Colletotrichum acutatum mycelial growth, conidial germination, appressoria formation, and in vivo on strawberry fruit disease incidence. All tested EOs, incorporated in potato-dextrose agar, inhibitedC. acutatum mycelial growth, and had a fungistatic effect at concentration 667 mu l/l of medium. Volatiles of cinnamon bark, thyme and clove bud EOs completely prevented conidial germination at the lowest concentrations of 1.53, 15.3 and 76.5 mu l/l of air, respectively, and disabled appressoria formation at concentration of 1.53 mu l/l of air. On inoculated strawberry fruit, thyme and cinnamon bark EO volatiles reduced anthracnose incidence at concentrations above 15.3 and 76.5 mu l/l of air, respectively. GC-FID and GC-MS analysis showed that major components of thyme EO were p-cymene, thymol, alpha-terpineol, carvacrol; cinnamon bark EO: trans- cinnameldehyde, trans-cinnamyl acetate; clove bud EO: eugenol and beta-caryophyllene. Our results suggest thatvolatiles of thyme and cinnamon bark EOs are effective against C. acutatum both in vitro and in vivo.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose",
pages = "537-529",
number = "3",
volume = "18",
doi = "10.1080/0972060X.2015.1004120"
}

Duduk, N., Marković, T., Vasić, M., Duduk, B., Vico, I.,& Obradović, A.. (2015). Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 18(3), 529-537.
https://doi.org/10.1080/0972060X.2015.1004120

Duduk N, Marković T, Vasić M, Duduk B, Vico I, Obradović A. Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose. in Journal of Essential Oil Bearing Plants. 2015;18(3):529-537.
doi:10.1080/0972060X.2015.1004120 .

Duduk, Nataša, Marković, Tatjana, Vasić, Miljan, Duduk, Bojan, Vico, Ivana, Obradović, Aleksa, "Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose" in Journal of Essential Oil Bearing Plants, 18, no. 3 (2015):529-537,
https://doi.org/10.1080/0972060X.2015.1004120 . .

TY  - JOUR
AU  - Lazarević, Marina
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Vico, Ivana
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3606
AB  - Penicillium polonicum is an economically important fungal species with a wide host range which includes cereals, peanuts, onions, dried meats, citrus, apple, and yam tubers. Onion bulbs cv. Meranto with blue mold symptoms have been collected, in a storage facility in Stara Pazova during 2013. Fungal isolates were obtained from decayed bulbs and their pathogenicity was proven after wound inoculation of healthy onion bulbs. On differential media the isolates formed velutinous, green blue colonies with a white margin and a characteristic odor. The isolates grew at 5 C while no growth was observed at 37 C. Isolates produced cyclopiazonic acid and other alkaloids. Conidiophores of isolates were terverticillate, stipes were septate with smooth to finely roughened walls, and phialides were ampulliform. Conidia were globose to subglobose, smooth-walled, and borne in columns. The nucleotide sequences of amplified products (~800 bp) for partial β-tubulin gene showed in MegaBLAST search analysis a 99% similarity with several sequences of P. polonicum. Based on morphological and molecular features isolates obtained from stored onion were identified as P. polonicum.
AB  - Penicillium polonicum je ekonomski važna gljiva sa širokim krugom domaćina, uključujući žitarice, kikiriki, lukove, sušeno meso, citruse, jabuke i krtole jama. U januaru 2013. godine, u skladištu na teritoriji Stare Pazove prikupljeni su uzorci lukovica crnog luka cv. Meranto sa karakterističnim simptomima plave truleži. Iz obolelih lukovica dobijeni su izolati gljive čija je patogenost potvrđena inokulacijom zdravih lukovica. Na diferencijalnim podlogama izolati su formirali dobro razvijene, guste kolonije, plavozelene boje sa belim obodom, karakterističnog mirisa. Izolati nisu rasli na 37oC, dok je porast zabeležen na 5oC. Utvrđeno je da su izolati stvarali ciklopiazonsku kiselinu i druge alkaloide. Konidiofore izolata su bile terverticilate, stipe septirane, tankih i glatkih zidova, a fijalide cilindrične. Konidije su bile loptaste do elipsoidne, glatke i formirale su se u dugim lancima. Sekvence dobijenih amplikona veličine oko 800 bp dela gena za β-tubulin su u MegaBLAST analizi pokazali najvišu sličnost od 99% sa sekvencama istog regiona vrste P. polonicum. Na osnovu morfoloških i molekularnih osobina kao prouzrokovač propadanja lukovica crnog luka u skladištu identifikovana je gljiva P. polonicum.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Penicillium polonicum: Causal agent of onion bulb decay in storage
T1  - Penicillium polonicum - prouzrokovač propadanja lukovica crnog luka u skladištu
EP  - 110
IS  - 3
SP  - 105
VL  - 65
DO  - 10.5937/zasbilj1403105L
ER  - 

@article{
author = "Lazarević, Marina and Duduk, Nataša and Vasić, Miljan and Vico, Ivana",
year = "2014",
abstract = "Penicillium polonicum is an economically important fungal species with a wide host range which includes cereals, peanuts, onions, dried meats, citrus, apple, and yam tubers. Onion bulbs cv. Meranto with blue mold symptoms have been collected, in a storage facility in Stara Pazova during 2013. Fungal isolates were obtained from decayed bulbs and their pathogenicity was proven after wound inoculation of healthy onion bulbs. On differential media the isolates formed velutinous, green blue colonies with a white margin and a characteristic odor. The isolates grew at 5 C while no growth was observed at 37 C. Isolates produced cyclopiazonic acid and other alkaloids. Conidiophores of isolates were terverticillate, stipes were septate with smooth to finely roughened walls, and phialides were ampulliform. Conidia were globose to subglobose, smooth-walled, and borne in columns. The nucleotide sequences of amplified products (~800 bp) for partial β-tubulin gene showed in MegaBLAST search analysis a 99% similarity with several sequences of P. polonicum. Based on morphological and molecular features isolates obtained from stored onion were identified as P. polonicum., Penicillium polonicum je ekonomski važna gljiva sa širokim krugom domaćina, uključujući žitarice, kikiriki, lukove, sušeno meso, citruse, jabuke i krtole jama. U januaru 2013. godine, u skladištu na teritoriji Stare Pazove prikupljeni su uzorci lukovica crnog luka cv. Meranto sa karakterističnim simptomima plave truleži. Iz obolelih lukovica dobijeni su izolati gljive čija je patogenost potvrđena inokulacijom zdravih lukovica. Na diferencijalnim podlogama izolati su formirali dobro razvijene, guste kolonije, plavozelene boje sa belim obodom, karakterističnog mirisa. Izolati nisu rasli na 37oC, dok je porast zabeležen na 5oC. Utvrđeno je da su izolati stvarali ciklopiazonsku kiselinu i druge alkaloide. Konidiofore izolata su bile terverticilate, stipe septirane, tankih i glatkih zidova, a fijalide cilindrične. Konidije su bile loptaste do elipsoidne, glatke i formirale su se u dugim lancima. Sekvence dobijenih amplikona veličine oko 800 bp dela gena za β-tubulin su u MegaBLAST analizi pokazali najvišu sličnost od 99% sa sekvencama istog regiona vrste P. polonicum. Na osnovu morfoloških i molekularnih osobina kao prouzrokovač propadanja lukovica crnog luka u skladištu identifikovana je gljiva P. polonicum.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Penicillium polonicum: Causal agent of onion bulb decay in storage, Penicillium polonicum - prouzrokovač propadanja lukovica crnog luka u skladištu",
pages = "110-105",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403105L"
}

Lazarević, M., Duduk, N., Vasić, M.,& Vico, I.. (2014). Penicillium polonicum: Causal agent of onion bulb decay in storage. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 65(3), 105-110.
https://doi.org/10.5937/zasbilj1403105L

Lazarević M, Duduk N, Vasić M, Vico I. Penicillium polonicum: Causal agent of onion bulb decay in storage. in Zaštita bilja. 2014;65(3):105-110.
doi:10.5937/zasbilj1403105L .

Lazarević, Marina, Duduk, Nataša, Vasić, Miljan, Vico, Ivana, "Penicillium polonicum: Causal agent of onion bulb decay in storage" in Zaštita bilja, 65, no. 3 (2014):105-110,
https://doi.org/10.5937/zasbilj1403105L . .

TY  - JOUR
AU  - Vico, Ivana
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Nikolić, Milica
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3561
AB  - Penicillium expansum (Link) Thom. is one of the most important postharvest pathogens of apple fruit worldwide. It causes blue mold, a decay that can lead to significant economic losses during storage, which can also impact fruit destined for processing due to the production of carcinogenic mycotoxin patulin. Apple fruit cvs. Idared, Golden Delicious and Braeburn with blue mold symptoms were collected from five storage facilities in Serbia and nine fungal isolates were obtained. Pathogenicity of the isolates was tested and proven by artificial inoculation of healthy apples cv. Idared. In order to identify the causal agents of decay, morphological and molecular methods were used. Colony morphology and microscopic features were observed on differential media, and isolates were tested for the production of cyclopiazonic acid. Molecular analysis included PCR amplification with species specific primers for P. expansum based on polygalacturonase gene (Pepg1), universal primers for internal transcribed spacer rDNA region and primers based on β-tubulin gene. All isolates formed compact blue green colonies with characteristic earthy odor. Conidiophores were terverticillate with smooth septate stipes and conidia were smooth, globose to subglobose, born in colums. The average size of conidia was 3.38 +- 0.49 (SD) x 3 +- 0.36 (SD) μm. Using species specific primers PEF/PER the texpected amplicons of ~404 bp were obtained in all nine tested isolates and PCR conducted with the Bt-LEVUp4/ Bt-LEV-Lo1 and universal ITS1/ITS4 primer pairs generated amplicons of the expected sizes of ~800 bp and ~600 bp, respectively. MegaBlast analyses of the 2X consensus of nucleotide sequences of the isolate JP1 partial β-tubulin gene and ITS region showed 99-100% and 100% similarity with several P. expansum sequences of corresponding regions of this species deposited in GenBank. Based on morphological and molecular features, the isolates obtained from decayed apple fruit collected in several storage facilities in Serbia were identified as P. expansum.
AB  - Penicillium expansum (Link) Thom. je jedan od najznačajnijih prouzrokovača truleži plodova jabučastog voća u toku čuvanja koji se odlikuje proizvodnjom mikotoksina patulina. U ovom radu je, na osnovu morfoloških i molekularnih osobina, identifikovana vrsta P. expansum kao prouzrokovač truleži plodova jabuke u skladištu. Plodovi jabuke sorti Ajdared, Zlatni delišes i Breburn sa simptomima truleži prikupljene su u skladištima u lokalitetima Pudarci, Suvodol, Čelarevo, Bela Crkva i Svilajnac. Na prikupljenim plodovima uočavale su se svetlosmeđe, meke i udubljene pege u okviru kojih je bila prisutna zelenoplava sporulacija gljive. Izolacija je obavljena standardnim postupkom na podlogu od krompir-dekstroznog agara (KDA), a patogenost izolata proverena je inokulacijom zdravih plodova jabuke. Morfološke odlike kolonija i reproduktivnih struktura izolata praćene su na dijagnostičkim hranljivim podlogama (MEA, CYA i YES). Molekularna identifikacija izolata obavljena je korišćenjem specifičnih prajmera za vrstu P. expansum zasnovanih na genu za poligalakturonazu (Pepg1), univerzalnih prajmera za ITS region i prajmera zasnovanih na genu za β-tubulin. Iz jabuke sa simptomima plave truleži dobijeno je devet izolata čija je patogenost potvrdjena veštačkim inokulacijama zdravih plodova jabuke. Ispitivani izolati su formirali kompaktne zelenoplave kolonije karakterističnog mirisa. Svi izolati su rasli na 5oC, a porasta nije bilo na 37oC. Konidije prosečne veličine 3,38 x 3 μm, bile su okruglaste do ovalne, formirane u dugim nizovima. Konidiofore su bile terverticillata, asimetričnog grananja sa glatkim stipama. Kod svih ispitivanih izolata amplikoni očekivane veličine oko 404 bp dobijeni su korišćenjem specifičnih prajmera za P. expansum PEF/PER, dok su primenom ITS1-ITS4 prajmera amplifikovani produkti veličine oko 600 bp, a primenom prajmera za deo β-tubulin gena, produkti veličine oko 800 bp. MegaBlast analizom dobijenih sekvenci ITS regiona i dela β-tubulin gena izabranog izolata JP1 utvrđena je 100%, odnosno 99-100% sličnost sa sekvencama odgovarajućih regiona vrste P. expansum deponovanim u NCBI bazi podataka. Na osnovu morfoloških i molekularnih osobina dobijenih izolata utvrđeno je da prouzrokovači truleži plodova jabuke u nekoliko skladišta u Srbiji pripadaju vrsti P. expansum.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit
T1  - Identifikacija Penicillium expansum prouzrokovača truleži plodova jabuke u skladištu
EP  - 266
IS  - 4
SP  - 257
VL  - 29
DO  - 10.2298/PIF1404257V
ER  - 

@article{
author = "Vico, Ivana and Duduk, Nataša and Vasić, Miljan and Nikolić, Milica",
year = "2014",
abstract = "Penicillium expansum (Link) Thom. is one of the most important postharvest pathogens of apple fruit worldwide. It causes blue mold, a decay that can lead to significant economic losses during storage, which can also impact fruit destined for processing due to the production of carcinogenic mycotoxin patulin. Apple fruit cvs. Idared, Golden Delicious and Braeburn with blue mold symptoms were collected from five storage facilities in Serbia and nine fungal isolates were obtained. Pathogenicity of the isolates was tested and proven by artificial inoculation of healthy apples cv. Idared. In order to identify the causal agents of decay, morphological and molecular methods were used. Colony morphology and microscopic features were observed on differential media, and isolates were tested for the production of cyclopiazonic acid. Molecular analysis included PCR amplification with species specific primers for P. expansum based on polygalacturonase gene (Pepg1), universal primers for internal transcribed spacer rDNA region and primers based on β-tubulin gene. All isolates formed compact blue green colonies with characteristic earthy odor. Conidiophores were terverticillate with smooth septate stipes and conidia were smooth, globose to subglobose, born in colums. The average size of conidia was 3.38 +- 0.49 (SD) x 3 +- 0.36 (SD) μm. Using species specific primers PEF/PER the texpected amplicons of ~404 bp were obtained in all nine tested isolates and PCR conducted with the Bt-LEVUp4/ Bt-LEV-Lo1 and universal ITS1/ITS4 primer pairs generated amplicons of the expected sizes of ~800 bp and ~600 bp, respectively. MegaBlast analyses of the 2X consensus of nucleotide sequences of the isolate JP1 partial β-tubulin gene and ITS region showed 99-100% and 100% similarity with several P. expansum sequences of corresponding regions of this species deposited in GenBank. Based on morphological and molecular features, the isolates obtained from decayed apple fruit collected in several storage facilities in Serbia were identified as P. expansum., Penicillium expansum (Link) Thom. je jedan od najznačajnijih prouzrokovača truleži plodova jabučastog voća u toku čuvanja koji se odlikuje proizvodnjom mikotoksina patulina. U ovom radu je, na osnovu morfoloških i molekularnih osobina, identifikovana vrsta P. expansum kao prouzrokovač truleži plodova jabuke u skladištu. Plodovi jabuke sorti Ajdared, Zlatni delišes i Breburn sa simptomima truleži prikupljene su u skladištima u lokalitetima Pudarci, Suvodol, Čelarevo, Bela Crkva i Svilajnac. Na prikupljenim plodovima uočavale su se svetlosmeđe, meke i udubljene pege u okviru kojih je bila prisutna zelenoplava sporulacija gljive. Izolacija je obavljena standardnim postupkom na podlogu od krompir-dekstroznog agara (KDA), a patogenost izolata proverena je inokulacijom zdravih plodova jabuke. Morfološke odlike kolonija i reproduktivnih struktura izolata praćene su na dijagnostičkim hranljivim podlogama (MEA, CYA i YES). Molekularna identifikacija izolata obavljena je korišćenjem specifičnih prajmera za vrstu P. expansum zasnovanih na genu za poligalakturonazu (Pepg1), univerzalnih prajmera za ITS region i prajmera zasnovanih na genu za β-tubulin. Iz jabuke sa simptomima plave truleži dobijeno je devet izolata čija je patogenost potvrdjena veštačkim inokulacijama zdravih plodova jabuke. Ispitivani izolati su formirali kompaktne zelenoplave kolonije karakterističnog mirisa. Svi izolati su rasli na 5oC, a porasta nije bilo na 37oC. Konidije prosečne veličine 3,38 x 3 μm, bile su okruglaste do ovalne, formirane u dugim nizovima. Konidiofore su bile terverticillata, asimetričnog grananja sa glatkim stipama. Kod svih ispitivanih izolata amplikoni očekivane veličine oko 404 bp dobijeni su korišćenjem specifičnih prajmera za P. expansum PEF/PER, dok su primenom ITS1-ITS4 prajmera amplifikovani produkti veličine oko 600 bp, a primenom prajmera za deo β-tubulin gena, produkti veličine oko 800 bp. MegaBlast analizom dobijenih sekvenci ITS regiona i dela β-tubulin gena izabranog izolata JP1 utvrđena je 100%, odnosno 99-100% sličnost sa sekvencama odgovarajućih regiona vrste P. expansum deponovanim u NCBI bazi podataka. Na osnovu morfoloških i molekularnih osobina dobijenih izolata utvrđeno je da prouzrokovači truleži plodova jabuke u nekoliko skladišta u Srbiji pripadaju vrsti P. expansum.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit, Identifikacija Penicillium expansum prouzrokovača truleži plodova jabuke u skladištu",
pages = "266-257",
number = "4",
volume = "29",
doi = "10.2298/PIF1404257V"
}

Vico, I., Duduk, N., Vasić, M.,& Nikolić, M.. (2014). Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 29(4), 257-266.
https://doi.org/10.2298/PIF1404257V

Vico I, Duduk N, Vasić M, Nikolić M. Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit. in Pesticidi i fitomedicina. 2014;29(4):257-266.
doi:10.2298/PIF1404257V .

Vico, Ivana, Duduk, Nataša, Vasić, Miljan, Nikolić, Milica, "Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit" in Pesticidi i fitomedicina, 29, no. 4 (2014):257-266,
https://doi.org/10.2298/PIF1404257V . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Vasić, M.
AU  - Vico, Ivana
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3405
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia
EP  - 1441
IS  - 10
SP  - 1440
VL  - 98
DO  - 10.1094/PDIS-05-14-0550-PDN
ER  - 

@article{
author = "Duduk, Nataša and Vasić, M. and Vico, Ivana",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia",
pages = "1441-1440",
number = "10",
volume = "98",
doi = "10.1094/PDIS-05-14-0550-PDN"
}

Duduk, N., Vasić, M.,& Vico, I.. (2014). First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(10), 1440-1441.
https://doi.org/10.1094/PDIS-05-14-0550-PDN

Duduk N, Vasić M, Vico I. First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia. in Plant Disease. 2014;98(10):1440-1441.
doi:10.1094/PDIS-05-14-0550-PDN .

Duduk, Nataša, Vasić, M., Vico, Ivana, "First Report of Penicillium polonicum Causing Blue Mold on Stored Onion (Allium cepa) in Serbia" in Plant Disease, 98, no. 10 (2014):1440-1441,
https://doi.org/10.1094/PDIS-05-14-0550-PDN . .

TY  - JOUR
AU  - Vico, Ivana
AU  - Gaskins, Verneta L.
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Yu, J.J.
AU  - Peter, K.A.
AU  - Jurick, Wayne M., II
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3403
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia
EP  - 1430
IS  - 10
SP  - 1430
VL  - 98
DO  - 10.1094/PDIS-02-14-0179-PDN
ER  - 

@article{
author = "Vico, Ivana and Gaskins, Verneta L. and Duduk, Nataša and Vasić, Miljan and Yu, J.J. and Peter, K.A. and Jurick, Wayne M., II",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia",
pages = "1430-1430",
number = "10",
volume = "98",
doi = "10.1094/PDIS-02-14-0179-PDN"
}

Vico, I., Gaskins, V. L., Duduk, N., Vasić, M., Yu, J.J., Peter, K.A.,& Jurick, W. M., I.. (2014). First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(10), 1430-1430.
https://doi.org/10.1094/PDIS-02-14-0179-PDN

Vico I, Gaskins VL, Duduk N, Vasić M, Yu J, Peter K, Jurick WMI. First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia. in Plant Disease. 2014;98(10):1430-1430.
doi:10.1094/PDIS-02-14-0179-PDN .

Vico, Ivana, Gaskins, Verneta L., Duduk, Nataša, Vasić, Miljan, Yu, J.J., Peter, K.A., Jurick, Wayne M., II, "First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia" in Plant Disease, 98, no. 10 (2014):1430-1430,
https://doi.org/10.1094/PDIS-02-14-0179-PDN . .

TY  - JOUR
AU  - Yan, H.J.
AU  - Gaskins, Verneta L.
AU  - Vico, Ivana
AU  - Luo, Y.G.
AU  - Jurick, Wayne M., II
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3649
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania
EP  - 1004
IS  - 7
SP  - 1004
VL  - 98
DO  - 10.1094/PDIS-12-13-1214-PDN
ER  - 

@article{
author = "Yan, H.J. and Gaskins, Verneta L. and Vico, Ivana and Luo, Y.G. and Jurick, Wayne M., II",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania",
pages = "1004-1004",
number = "7",
volume = "98",
doi = "10.1094/PDIS-12-13-1214-PDN"
}

Yan, H.J., Gaskins, V. L., Vico, I., Luo, Y.G.,& Jurick, W. M., I.. (2014). First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(7), 1004-1004.
https://doi.org/10.1094/PDIS-12-13-1214-PDN

Yan H, Gaskins VL, Vico I, Luo Y, Jurick WMI. First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania. in Plant Disease. 2014;98(7):1004-1004.
doi:10.1094/PDIS-12-13-1214-PDN .

Yan, H.J., Gaskins, Verneta L., Vico, Ivana, Luo, Y.G., Jurick, Wayne M., II, "First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania" in Plant Disease, 98, no. 7 (2014):1004-1004,
https://doi.org/10.1094/PDIS-12-13-1214-PDN . .