TY  - JOUR
AU  - Vasić, Miljan
AU  - Vico, Ivana
AU  - Jurick, Wayne M.
AU  - Duduk, Bojan
AU  - Duduk, Nataša
PY  - 2022
UR  - https://doi.org/10.1007/s11557-022-01841-w
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6197
AB  - Lambertella corni-maris was isolated from a dark brown lesion on apple fruit cv. Golden Delicious collected from an orchard, and from stored apple fruit cv. Idared previously colonized with Monilia polystroma. The contrasting origin of the isolates led us to hypothesize that this fungus behaves differently in various contexts, which triggered the investigation of L. corni-maris as a pathogen and antagonist. Following identification based on cultural and molecular characteristics, isolates of different origin were characterized and their cultural, ecological, pathogenic, and antagonistic features were compared. This study has shown that L. corni-maris, originally isolated as an apple fruit pathogen and one as an antagonist of M. polystroma, have similar features in vitro and in vivo. The isolates vary widely in growth rate, cultural morphology, crystal production, intensity of dark zone and co-antagonism with two Monilinia species but can also function as apple fruit pathogens and inhibit and replace different Monilinia spp. on apple fruit. These findings reinforce their dual nature in different host-pathogen interactions. The data from this study are biologically intriguing and practically relevant, and have translative potential to exploit L. corni-maris as a model system for developing strategies to block brown rot of apple and to pinpoint virulence factors in the fungus for yellow rot management.
T2  - Mycological Progress
T2  - Mycological ProgressMycol Progress
T1  - The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp
IS  - 11
SP  - 91
VL  - 21
DO  - 10.1007/s11557-022-01841-w
ER  - 

@article{
author = "Vasić, Miljan and Vico, Ivana and Jurick, Wayne M. and Duduk, Bojan and Duduk, Nataša",
year = "2022",
abstract = "Lambertella corni-maris was isolated from a dark brown lesion on apple fruit cv. Golden Delicious collected from an orchard, and from stored apple fruit cv. Idared previously colonized with Monilia polystroma. The contrasting origin of the isolates led us to hypothesize that this fungus behaves differently in various contexts, which triggered the investigation of L. corni-maris as a pathogen and antagonist. Following identification based on cultural and molecular characteristics, isolates of different origin were characterized and their cultural, ecological, pathogenic, and antagonistic features were compared. This study has shown that L. corni-maris, originally isolated as an apple fruit pathogen and one as an antagonist of M. polystroma, have similar features in vitro and in vivo. The isolates vary widely in growth rate, cultural morphology, crystal production, intensity of dark zone and co-antagonism with two Monilinia species but can also function as apple fruit pathogens and inhibit and replace different Monilinia spp. on apple fruit. These findings reinforce their dual nature in different host-pathogen interactions. The data from this study are biologically intriguing and practically relevant, and have translative potential to exploit L. corni-maris as a model system for developing strategies to block brown rot of apple and to pinpoint virulence factors in the fungus for yellow rot management.",
journal = "Mycological Progress, Mycological ProgressMycol Progress",
title = "The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp",
number = "11",
pages = "91",
volume = "21",
doi = "10.1007/s11557-022-01841-w"
}

Vasić, M., Vico, I., Jurick, W. M., Duduk, B.,& Duduk, N.. (2022). The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp. in Mycological Progress, 21(11), 91.
https://doi.org/10.1007/s11557-022-01841-w

Vasić M, Vico I, Jurick WM, Duduk B, Duduk N. The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp. in Mycological Progress. 2022;21(11):91.
doi:10.1007/s11557-022-01841-w .

Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., Duduk, Bojan, Duduk, Nataša, "The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp" in Mycological Progress, 21, no. 11 (2022):91,
https://doi.org/10.1007/s11557-022-01841-w . .

TY  - JOUR
AU  - Vasić, Miljan
AU  - Vico, Ivana
AU  - Jurick, Wayne M., II
AU  - Duduk, Nataša
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4741
AB  - Brown rot, caused by Monilinia spp., is an economically important pre and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia
EP  - 369
IS  - 2
SP  - 359
VL  - 102
DO  - 10.1094/PDIS-06-17-0867-RE
ER  - 

@article{
author = "Vasić, Miljan and Vico, Ivana and Jurick, Wayne M., II and Duduk, Nataša",
year = "2018",
abstract = "Brown rot, caused by Monilinia spp., is an economically important pre and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia",
pages = "369-359",
number = "2",
volume = "102",
doi = "10.1094/PDIS-06-17-0867-RE"
}

Vasić, M., Vico, I., Jurick, W. M., I.,& Duduk, N.. (2018). Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 102(2), 359-369.
https://doi.org/10.1094/PDIS-06-17-0867-RE

Vasić M, Vico I, Jurick WMI, Duduk N. Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia. in Plant Disease. 2018;102(2):359-369.
doi:10.1094/PDIS-06-17-0867-RE .

Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., II, Duduk, Nataša, "Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia" in Plant Disease, 102, no. 2 (2018):359-369,
https://doi.org/10.1094/PDIS-06-17-0867-RE . .

TY  - CONF
AU  - Jurick, Wayne M., II
AU  - Macarisin, O.
AU  - Gaskins, Verneta L.
AU  - Vico, Ivana
AU  - Yu, J.
AU  - Peter, K.A.
AU  - Janisiewicz, Wojciech
AU  - Cox, K.D.
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4443
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management
EP  - 63
IS  - 12
SP  - 63
VL  - 107
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4443
ER  - 

@conference{
author = "Jurick, Wayne M., II and Macarisin, O. and Gaskins, Verneta L. and Vico, Ivana and Yu, J. and Peter, K.A. and Janisiewicz, Wojciech and Cox, K.D.",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management",
pages = "63-63",
number = "12",
volume = "107",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4443"
}

Jurick, W. M., I., Macarisin, O., Gaskins, V. L., Vico, I., Yu, J., Peter, K.A., Janisiewicz, W.,& Cox, K.D.. (2017). Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management. in Phytopathology
Amer Phytopathological Soc, St Paul., 107(12), 63-63.
https://hdl.handle.net/21.15107/rcub_agrospace_4443

Jurick WMI, Macarisin O, Gaskins VL, Vico I, Yu J, Peter K, Janisiewicz W, Cox K. Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management. in Phytopathology. 2017;107(12):63-63.
https://hdl.handle.net/21.15107/rcub_agrospace_4443 .

Jurick, Wayne M., II, Macarisin, O., Gaskins, Verneta L., Vico, Ivana, Yu, J., Peter, K.A., Janisiewicz, Wojciech, Cox, K.D., "Botrytis cinerea isolates lacking the Mrr1 R632I mutation are multi-resistant to postharvest fungicides which impacts apple grey mold management" in Phytopathology, 107, no. 12 (2017):63-63,
https://hdl.handle.net/21.15107/rcub_agrospace_4443 .

TY  - JOUR
AU  - Vico, Ivana
AU  - Gaskins, Verneta L.
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Yu, J.J.
AU  - Peter, K.A.
AU  - Jurick, Wayne M., II
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3403
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia
EP  - 1430
IS  - 10
SP  - 1430
VL  - 98
DO  - 10.1094/PDIS-02-14-0179-PDN
ER  - 

@article{
author = "Vico, Ivana and Gaskins, Verneta L. and Duduk, Nataša and Vasić, Miljan and Yu, J.J. and Peter, K.A. and Jurick, Wayne M., II",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia",
pages = "1430-1430",
number = "10",
volume = "98",
doi = "10.1094/PDIS-02-14-0179-PDN"
}

Vico, I., Gaskins, V. L., Duduk, N., Vasić, M., Yu, J.J., Peter, K.A.,& Jurick, W. M., I.. (2014). First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(10), 1430-1430.
https://doi.org/10.1094/PDIS-02-14-0179-PDN

Vico I, Gaskins VL, Duduk N, Vasić M, Yu J, Peter K, Jurick WMI. First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia. in Plant Disease. 2014;98(10):1430-1430.
doi:10.1094/PDIS-02-14-0179-PDN .

Vico, Ivana, Gaskins, Verneta L., Duduk, Nataša, Vasić, Miljan, Yu, J.J., Peter, K.A., Jurick, Wayne M., II, "First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia" in Plant Disease, 98, no. 10 (2014):1430-1430,
https://doi.org/10.1094/PDIS-02-14-0179-PDN . .

TY  - JOUR
AU  - Yan, H.J.
AU  - Gaskins, Verneta L.
AU  - Vico, Ivana
AU  - Luo, Y.G.
AU  - Jurick, Wayne M., II
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3649
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania
EP  - 1004
IS  - 7
SP  - 1004
VL  - 98
DO  - 10.1094/PDIS-12-13-1214-PDN
ER  - 

@article{
author = "Yan, H.J. and Gaskins, Verneta L. and Vico, Ivana and Luo, Y.G. and Jurick, Wayne M., II",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania",
pages = "1004-1004",
number = "7",
volume = "98",
doi = "10.1094/PDIS-12-13-1214-PDN"
}

Yan, H.J., Gaskins, V. L., Vico, I., Luo, Y.G.,& Jurick, W. M., I.. (2014). First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(7), 1004-1004.
https://doi.org/10.1094/PDIS-12-13-1214-PDN

Yan H, Gaskins VL, Vico I, Luo Y, Jurick WMI. First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania. in Plant Disease. 2014;98(7):1004-1004.
doi:10.1094/PDIS-12-13-1214-PDN .

Yan, H.J., Gaskins, Verneta L., Vico, Ivana, Luo, Y.G., Jurick, Wayne M., II, "First Report of Penicillium expansum Isolates Resistant to Pyrimethanil from Stored Apple Fruit in Pennsylvania" in Plant Disease, 98, no. 7 (2014):1004-1004,
https://doi.org/10.1094/PDIS-12-13-1214-PDN . .

TY  - CONF
AU  - Peter, K.
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - Park, E.
AU  - Gaskins, Verneta L.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2664
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Host modification of Penicillium solitum during postharvest decay of apple fruit
EP  - S141
IS  - 6
SP  - S141
VL  - 101
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2664
ER  - 

@conference{
author = "Peter, K. and Jurick, Wayne M., II and Vico, Ivana and Park, E. and Gaskins, Verneta L. and Janisiewicz, Wojciech and Conway, William S.",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Host modification of Penicillium solitum during postharvest decay of apple fruit",
pages = "S141-S141",
number = "6",
volume = "101",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2664"
}

Peter, K., Jurick, W. M., I., Vico, I., Park, E., Gaskins, V. L., Janisiewicz, W.,& Conway, W. S.. (2011). Host modification of Penicillium solitum during postharvest decay of apple fruit. in Phytopathology
Amer Phytopathological Soc, St Paul., 101(6), S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_2664

Peter K, Jurick WMI, Vico I, Park E, Gaskins VL, Janisiewicz W, Conway WS. Host modification of Penicillium solitum during postharvest decay of apple fruit. in Phytopathology. 2011;101(6):S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_2664 .

Peter, K., Jurick, Wayne M., II, Vico, Ivana, Park, E., Gaskins, Verneta L., Janisiewicz, Wojciech, Conway, William S., "Host modification of Penicillium solitum during postharvest decay of apple fruit" in Phytopathology, 101, no. 6 (2011):S141-S141,
https://hdl.handle.net/21.15107/rcub_agrospace_2664 .

TY  - JOUR
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - Gaskins, Verneta L.
AU  - Garrett, Wesley M.
AU  - Whitaker, Bruce D.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2375
AB  - A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified PG is of fungal origin. The purified enzyme had a molecular mass of 41 kDa and a pI of 7.8. Activity of the PG was not associated with a glycosylated protein. The enzyme was active over a broad pH range from 3 to 6, with optimal activity at 4.5 in sodium citrate and sodium acetate buffers. The optimal temperature for activity was 37 degrees C but the enzyme was also active at 0, 5, 10, 20, and 50 degrees C. Thin-layer chromatographic analysis of PG hydrolysis products showed that the enzyme exhibits endo- and exo-activity. The purified enzyme macerated tissue in vitro causing approximate to 30% reduction in mass of pear plugs compared with approximate to 17% reduction for apple. Additionally, it produced 1.5-fold more soluble polyuronides on pear than apple tissue. This work shows for the first time the production of a PG by P. expansum during postharvest decay of pear fruit is different from the previously described PG produced in decayed apple fruit by the same pathogen.
PB  - Amer Phytopathological Soc, St Paul
T2  - Phytopathology
T1  - Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear
EP  - 48
IS  - 1
SP  - 42
VL  - 100
DO  - 10.1094/PHYTO-100-1-0042
ER  - 

@article{
author = "Jurick, Wayne M., II and Vico, Ivana and Gaskins, Verneta L. and Garrett, Wesley M. and Whitaker, Bruce D. and Janisiewicz, Wojciech and Conway, William S.",
year = "2010",
abstract = "A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified PG is of fungal origin. The purified enzyme had a molecular mass of 41 kDa and a pI of 7.8. Activity of the PG was not associated with a glycosylated protein. The enzyme was active over a broad pH range from 3 to 6, with optimal activity at 4.5 in sodium citrate and sodium acetate buffers. The optimal temperature for activity was 37 degrees C but the enzyme was also active at 0, 5, 10, 20, and 50 degrees C. Thin-layer chromatographic analysis of PG hydrolysis products showed that the enzyme exhibits endo- and exo-activity. The purified enzyme macerated tissue in vitro causing approximate to 30% reduction in mass of pear plugs compared with approximate to 17% reduction for apple. Additionally, it produced 1.5-fold more soluble polyuronides on pear than apple tissue. This work shows for the first time the production of a PG by P. expansum during postharvest decay of pear fruit is different from the previously described PG produced in decayed apple fruit by the same pathogen.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear",
pages = "48-42",
number = "1",
volume = "100",
doi = "10.1094/PHYTO-100-1-0042"
}

Jurick, W. M., I., Vico, I., Gaskins, V. L., Garrett, W. M., Whitaker, B. D., Janisiewicz, W.,& Conway, W. S.. (2010). Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear. in Phytopathology
Amer Phytopathological Soc, St Paul., 100(1), 42-48.
https://doi.org/10.1094/PHYTO-100-1-0042

Jurick WMI, Vico I, Gaskins VL, Garrett WM, Whitaker BD, Janisiewicz W, Conway WS. Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear. in Phytopathology. 2010;100(1):42-48.
doi:10.1094/PHYTO-100-1-0042 .

Jurick, Wayne M., II, Vico, Ivana, Gaskins, Verneta L., Garrett, Wesley M., Whitaker, Bruce D., Janisiewicz, Wojciech, Conway, William S., "Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear" in Phytopathology, 100, no. 1 (2010):42-48,
https://doi.org/10.1094/PHYTO-100-1-0042 . .

TY  - JOUR
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - McEvoy, James L.
AU  - Whitaker, Bruce D.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1987
AB  - Polygalacturonase (PG) was extracted and purified from decayed 'Golden Delicious' apple fruit inoculated with Penicillium solitum. Ammonium sulfate, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. The purified PG most likely originates from the fungus because PG activity from healthy and wounded apple tissue was undetectable. Analysis of cation exchange-purified material using sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single 50-kDa band. The enzyme was active over a broad pH range (3 to 7), with optimal activity between pH 4 and 5. PG was highly active at 20 and 37 degrees C but was also detectable at 2, 50, and 75 degrees C. Divalent cations affected PG enzyme activity; Mg and Fe increased, whereas Ca and Mn reduced activity in vitro. Thin-layer chromatographic separation of hydrolysis products and data from a PG plate activity assay based on staining with ruthenium red showed that the enzyme exhibits both exo and endo activity. Purified PG incubated with intact apple fruit tissue in vitro caused a 30% reduction in mass after 48 h, suggesting a role in P. solitum-mediated decay of apple fruit.
PB  - Amer Phytopathological Soc, St Paul
T2  - Phytopathology
T1  - Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit
EP  - 641
IS  - 6
SP  - 636
VL  - 99
DO  - 10.1094/PHYTO-99-6-0636
ER  - 

@article{
author = "Jurick, Wayne M., II and Vico, Ivana and McEvoy, James L. and Whitaker, Bruce D. and Janisiewicz, Wojciech and Conway, William S.",
year = "2009",
abstract = "Polygalacturonase (PG) was extracted and purified from decayed 'Golden Delicious' apple fruit inoculated with Penicillium solitum. Ammonium sulfate, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. The purified PG most likely originates from the fungus because PG activity from healthy and wounded apple tissue was undetectable. Analysis of cation exchange-purified material using sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single 50-kDa band. The enzyme was active over a broad pH range (3 to 7), with optimal activity between pH 4 and 5. PG was highly active at 20 and 37 degrees C but was also detectable at 2, 50, and 75 degrees C. Divalent cations affected PG enzyme activity; Mg and Fe increased, whereas Ca and Mn reduced activity in vitro. Thin-layer chromatographic separation of hydrolysis products and data from a PG plate activity assay based on staining with ruthenium red showed that the enzyme exhibits both exo and endo activity. Purified PG incubated with intact apple fruit tissue in vitro caused a 30% reduction in mass after 48 h, suggesting a role in P. solitum-mediated decay of apple fruit.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit",
pages = "641-636",
number = "6",
volume = "99",
doi = "10.1094/PHYTO-99-6-0636"
}

Jurick, W. M., I., Vico, I., McEvoy, J. L., Whitaker, B. D., Janisiewicz, W.,& Conway, W. S.. (2009). Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit. in Phytopathology
Amer Phytopathological Soc, St Paul., 99(6), 636-641.
https://doi.org/10.1094/PHYTO-99-6-0636

Jurick WMI, Vico I, McEvoy JL, Whitaker BD, Janisiewicz W, Conway WS. Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit. in Phytopathology. 2009;99(6):636-641.
doi:10.1094/PHYTO-99-6-0636 .

Jurick, Wayne M., II, Vico, Ivana, McEvoy, James L., Whitaker, Bruce D., Janisiewicz, Wojciech, Conway, William S., "Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit" in Phytopathology, 99, no. 6 (2009):636-641,
https://doi.org/10.1094/PHYTO-99-6-0636 . .

TY  - CONF
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - McEvoy, J.L.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1806
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh)
EP  - S77
IS  - 6
SP  - S77
VL  - 98
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1806
ER  - 

@conference{
author = "Jurick, Wayne M., II and Vico, Ivana and McEvoy, J.L. and Janisiewicz, Wojciech and Conway, William S.",
year = "2008",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh)",
pages = "S77-S77",
number = "6",
volume = "98",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1806"
}

Jurick, W. M., I., Vico, I., McEvoy, J.L., Janisiewicz, W.,& Conway, W. S.. (2008). Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh). in Phytopathology
Amer Phytopathological Soc, St Paul., 98(6), S77-S77.
https://hdl.handle.net/21.15107/rcub_agrospace_1806

Jurick WMI, Vico I, McEvoy J, Janisiewicz W, Conway WS. Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh). in Phytopathology. 2008;98(6):S77-S77.
https://hdl.handle.net/21.15107/rcub_agrospace_1806 .

Jurick, Wayne M., II, Vico, Ivana, McEvoy, J.L., Janisiewicz, Wojciech, Conway, William S., "Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh)" in Phytopathology, 98, no. 6 (2008):S77-S77,
https://hdl.handle.net/21.15107/rcub_agrospace_1806 .