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Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology

Authorized Users Only
2016
Authors
Vasić, M.
Duduk, Nataša
Vico, Ivana
Rančić, Dragana
Pajić, V.
Backhouse, D.
Article (Published version)
Metadata
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Abstract
In this study, we compared cultural, morphological, pathogenic and biological features of Serbian isolates of Monilia polystroma and Monilinia fructigena from apple fruit. Isolates of M. polystroma formed characteristic stromatal plates throughout the cultures unlike M. fructigena, while mycelial growth rates were not distinguishable features between species. Conidia of M. fructigena were larger than M. polystroma, and both species produced slightly larger conidia on apple fruit than on V8 medium. The existence and morphology of the microconidial state of M. polystroma was described for the first time using light and SEM microscopy. Microconidia were globose (2.19-3.44 mu m), similar to M. fructigena. Digestion of polymerase chain reaction (PCR) product of ribosomal ITS1-5.8S-ITS2 region with restriction enzyme HhaI provided a reliable method for specific detection of M. polystroma. In a pathogenicity test, both species produced larger lesions on cv. Golden Delicious, than on cvs. Idar...ed and Granny Smith. On cv. Golden Delicious, the mean lesion size was not significatly different between species, while on cvs. Idared and Granny Smith M. fructigena tended to produce slightly larger lesions compared to M. polystroma. On artificially inoculated apple fruit after 1 and 4 months of incubation, M. polystroma, unlike M. fructigena, was capable of forming thicker and more compact stromata with complex morphology, which consisted of densely interwoven medullary hyphae covered with a discontinuous layer of melanized hyphal rind cells. The dynamics of stromata formation beneath the host's cuticle also showed differences between two species, where M. polystroma formed this layer after 1 month, while M. fructigena after 4 months of incubation.

Keywords:
Comparison / Monilia polystroma / Monilinia fructigena / PCR-RFLP / Pathogenicity / Histopathology
Source:
European Journal of Plant Pathology, 2016, 144, 1, 15-30
Publisher:
  • Springer, Dordrecht
Funding / projects:
  • Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-46008)
  • Biotechnological approaches for overcoming effects of drought on agricultural production in Serbia (RS-31005)

DOI: 10.1007/s10658-015-0740-6

ISSN: 0929-1873

WoS: 000366635400002

Scopus: 2-s2.0-84951569185
[ Google Scholar ]
8
7
URI
http://aspace.agrif.bg.ac.rs/handle/123456789/4135
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Poljoprivredni fakultet
TY  - JOUR
AU  - Vasić, M.
AU  - Duduk, Nataša
AU  - Vico, Ivana
AU  - Rančić, Dragana
AU  - Pajić, V.
AU  - Backhouse, D.
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4135
AB  - In this study, we compared cultural, morphological, pathogenic and biological features of Serbian isolates of Monilia polystroma and Monilinia fructigena from apple fruit. Isolates of M. polystroma formed characteristic stromatal plates throughout the cultures unlike M. fructigena, while mycelial growth rates were not distinguishable features between species. Conidia of M. fructigena were larger than M. polystroma, and both species produced slightly larger conidia on apple fruit than on V8 medium. The existence and morphology of the microconidial state of M. polystroma was described for the first time using light and SEM microscopy. Microconidia were globose (2.19-3.44 mu m), similar to M. fructigena. Digestion of polymerase chain reaction (PCR) product of ribosomal ITS1-5.8S-ITS2 region with restriction enzyme HhaI provided a reliable method for specific detection of M. polystroma. In a pathogenicity test, both species produced larger lesions on cv. Golden Delicious, than on cvs. Idared and Granny Smith. On cv. Golden Delicious, the mean lesion size was not significatly different between species, while on cvs. Idared and Granny Smith M. fructigena tended to produce slightly larger lesions compared to M. polystroma. On artificially inoculated apple fruit after 1 and 4 months of incubation, M. polystroma, unlike M. fructigena, was capable of forming thicker and more compact stromata with complex morphology, which consisted of densely interwoven medullary hyphae covered with a discontinuous layer of melanized hyphal rind cells. The dynamics of stromata formation beneath the host's cuticle also showed differences between two species, where M. polystroma formed this layer after 1 month, while M. fructigena after 4 months of incubation.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology
EP  - 30
IS  - 1
SP  - 15
VL  - 144
DO  - 10.1007/s10658-015-0740-6
UR  - conv_5635
ER  - 
@article{
author = "Vasić, M. and Duduk, Nataša and Vico, Ivana and Rančić, Dragana and Pajić, V. and Backhouse, D.",
year = "2016",
abstract = "In this study, we compared cultural, morphological, pathogenic and biological features of Serbian isolates of Monilia polystroma and Monilinia fructigena from apple fruit. Isolates of M. polystroma formed characteristic stromatal plates throughout the cultures unlike M. fructigena, while mycelial growth rates were not distinguishable features between species. Conidia of M. fructigena were larger than M. polystroma, and both species produced slightly larger conidia on apple fruit than on V8 medium. The existence and morphology of the microconidial state of M. polystroma was described for the first time using light and SEM microscopy. Microconidia were globose (2.19-3.44 mu m), similar to M. fructigena. Digestion of polymerase chain reaction (PCR) product of ribosomal ITS1-5.8S-ITS2 region with restriction enzyme HhaI provided a reliable method for specific detection of M. polystroma. In a pathogenicity test, both species produced larger lesions on cv. Golden Delicious, than on cvs. Idared and Granny Smith. On cv. Golden Delicious, the mean lesion size was not significatly different between species, while on cvs. Idared and Granny Smith M. fructigena tended to produce slightly larger lesions compared to M. polystroma. On artificially inoculated apple fruit after 1 and 4 months of incubation, M. polystroma, unlike M. fructigena, was capable of forming thicker and more compact stromata with complex morphology, which consisted of densely interwoven medullary hyphae covered with a discontinuous layer of melanized hyphal rind cells. The dynamics of stromata formation beneath the host's cuticle also showed differences between two species, where M. polystroma formed this layer after 1 month, while M. fructigena after 4 months of incubation.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology",
pages = "30-15",
number = "1",
volume = "144",
doi = "10.1007/s10658-015-0740-6",
url = "conv_5635"
}
Vasić, M., Duduk, N., Vico, I., Rančić, D., Pajić, V.,& Backhouse, D.. (2016). Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology. in European Journal of Plant Pathology
Springer, Dordrecht., 144(1), 15-30.
https://doi.org/10.1007/s10658-015-0740-6
conv_5635
Vasić M, Duduk N, Vico I, Rančić D, Pajić V, Backhouse D. Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology. in European Journal of Plant Pathology. 2016;144(1):15-30.
doi:10.1007/s10658-015-0740-6
conv_5635 .
Vasić, M., Duduk, Nataša, Vico, Ivana, Rančić, Dragana, Pajić, V., Backhouse, D., "Comparative study of Monilinia fructigena and Monilia polystroma on morphological features, RFLP analysis, pathogenicity and histopathology" in European Journal of Plant Pathology, 144, no. 1 (2016):15-30,
https://doi.org/10.1007/s10658-015-0740-6 .,
conv_5635 .

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