TY  - JOUR
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - Gaskins, Verneta L.
AU  - Garrett, Wesley M.
AU  - Whitaker, Bruce D.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2375
AB  - A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified PG is of fungal origin. The purified enzyme had a molecular mass of 41 kDa and a pI of 7.8. Activity of the PG was not associated with a glycosylated protein. The enzyme was active over a broad pH range from 3 to 6, with optimal activity at 4.5 in sodium citrate and sodium acetate buffers. The optimal temperature for activity was 37 degrees C but the enzyme was also active at 0, 5, 10, 20, and 50 degrees C. Thin-layer chromatographic analysis of PG hydrolysis products showed that the enzyme exhibits endo- and exo-activity. The purified enzyme macerated tissue in vitro causing approximate to 30% reduction in mass of pear plugs compared with approximate to 17% reduction for apple. Additionally, it produced 1.5-fold more soluble polyuronides on pear than apple tissue. This work shows for the first time the production of a PG by P. expansum during postharvest decay of pear fruit is different from the previously described PG produced in decayed apple fruit by the same pathogen.
PB  - Amer Phytopathological Soc, St Paul
T2  - Phytopathology
T1  - Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear
EP  - 48
IS  - 1
SP  - 42
VL  - 100
DO  - 10.1094/PHYTO-100-1-0042
ER  - 

@article{
author = "Jurick, Wayne M., II and Vico, Ivana and Gaskins, Verneta L. and Garrett, Wesley M. and Whitaker, Bruce D. and Janisiewicz, Wojciech and Conway, William S.",
year = "2010",
abstract = "A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified PG is of fungal origin. The purified enzyme had a molecular mass of 41 kDa and a pI of 7.8. Activity of the PG was not associated with a glycosylated protein. The enzyme was active over a broad pH range from 3 to 6, with optimal activity at 4.5 in sodium citrate and sodium acetate buffers. The optimal temperature for activity was 37 degrees C but the enzyme was also active at 0, 5, 10, 20, and 50 degrees C. Thin-layer chromatographic analysis of PG hydrolysis products showed that the enzyme exhibits endo- and exo-activity. The purified enzyme macerated tissue in vitro causing approximate to 30% reduction in mass of pear plugs compared with approximate to 17% reduction for apple. Additionally, it produced 1.5-fold more soluble polyuronides on pear than apple tissue. This work shows for the first time the production of a PG by P. expansum during postharvest decay of pear fruit is different from the previously described PG produced in decayed apple fruit by the same pathogen.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear",
pages = "48-42",
number = "1",
volume = "100",
doi = "10.1094/PHYTO-100-1-0042"
}

Jurick, W. M., I., Vico, I., Gaskins, V. L., Garrett, W. M., Whitaker, B. D., Janisiewicz, W.,& Conway, W. S.. (2010). Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear. in Phytopathology
Amer Phytopathological Soc, St Paul., 100(1), 42-48.
https://doi.org/10.1094/PHYTO-100-1-0042

Jurick WMI, Vico I, Gaskins VL, Garrett WM, Whitaker BD, Janisiewicz W, Conway WS. Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear. in Phytopathology. 2010;100(1):42-48.
doi:10.1094/PHYTO-100-1-0042 .

Jurick, Wayne M., II, Vico, Ivana, Gaskins, Verneta L., Garrett, Wesley M., Whitaker, Bruce D., Janisiewicz, Wojciech, Conway, William S., "Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear" in Phytopathology, 100, no. 1 (2010):42-48,
https://doi.org/10.1094/PHYTO-100-1-0042 . .