TY  - JOUR
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - McEvoy, James L.
AU  - Whitaker, Bruce D.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1987
AB  - Polygalacturonase (PG) was extracted and purified from decayed 'Golden Delicious' apple fruit inoculated with Penicillium solitum. Ammonium sulfate, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. The purified PG most likely originates from the fungus because PG activity from healthy and wounded apple tissue was undetectable. Analysis of cation exchange-purified material using sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single 50-kDa band. The enzyme was active over a broad pH range (3 to 7), with optimal activity between pH 4 and 5. PG was highly active at 20 and 37 degrees C but was also detectable at 2, 50, and 75 degrees C. Divalent cations affected PG enzyme activity; Mg and Fe increased, whereas Ca and Mn reduced activity in vitro. Thin-layer chromatographic separation of hydrolysis products and data from a PG plate activity assay based on staining with ruthenium red showed that the enzyme exhibits both exo and endo activity. Purified PG incubated with intact apple fruit tissue in vitro caused a 30% reduction in mass after 48 h, suggesting a role in P. solitum-mediated decay of apple fruit.
PB  - Amer Phytopathological Soc, St Paul
T2  - Phytopathology
T1  - Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit
EP  - 641
IS  - 6
SP  - 636
VL  - 99
DO  - 10.1094/PHYTO-99-6-0636
ER  - 

@article{
author = "Jurick, Wayne M., II and Vico, Ivana and McEvoy, James L. and Whitaker, Bruce D. and Janisiewicz, Wojciech and Conway, William S.",
year = "2009",
abstract = "Polygalacturonase (PG) was extracted and purified from decayed 'Golden Delicious' apple fruit inoculated with Penicillium solitum. Ammonium sulfate, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. The purified PG most likely originates from the fungus because PG activity from healthy and wounded apple tissue was undetectable. Analysis of cation exchange-purified material using sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single 50-kDa band. The enzyme was active over a broad pH range (3 to 7), with optimal activity between pH 4 and 5. PG was highly active at 20 and 37 degrees C but was also detectable at 2, 50, and 75 degrees C. Divalent cations affected PG enzyme activity; Mg and Fe increased, whereas Ca and Mn reduced activity in vitro. Thin-layer chromatographic separation of hydrolysis products and data from a PG plate activity assay based on staining with ruthenium red showed that the enzyme exhibits both exo and endo activity. Purified PG incubated with intact apple fruit tissue in vitro caused a 30% reduction in mass after 48 h, suggesting a role in P. solitum-mediated decay of apple fruit.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit",
pages = "641-636",
number = "6",
volume = "99",
doi = "10.1094/PHYTO-99-6-0636"
}

Jurick, W. M., I., Vico, I., McEvoy, J. L., Whitaker, B. D., Janisiewicz, W.,& Conway, W. S.. (2009). Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit. in Phytopathology
Amer Phytopathological Soc, St Paul., 99(6), 636-641.
https://doi.org/10.1094/PHYTO-99-6-0636

Jurick WMI, Vico I, McEvoy JL, Whitaker BD, Janisiewicz W, Conway WS. Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit. in Phytopathology. 2009;99(6):636-641.
doi:10.1094/PHYTO-99-6-0636 .

Jurick, Wayne M., II, Vico, Ivana, McEvoy, James L., Whitaker, Bruce D., Janisiewicz, Wojciech, Conway, William S., "Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit" in Phytopathology, 99, no. 6 (2009):636-641,
https://doi.org/10.1094/PHYTO-99-6-0636 . .