TY  - JOUR
AU  - Vasić, Miljan
AU  - Vico, Ivana
AU  - Jurick, Wayne M.
AU  - Duduk, Bojan
AU  - Duduk, Nataša
PY  - 2022
UR  - https://doi.org/10.1007/s11557-022-01841-w
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6197
AB  - Lambertella corni-maris was isolated from a dark brown lesion on apple fruit cv. Golden Delicious collected from an orchard, and from stored apple fruit cv. Idared previously colonized with Monilia polystroma. The contrasting origin of the isolates led us to hypothesize that this fungus behaves differently in various contexts, which triggered the investigation of L. corni-maris as a pathogen and antagonist. Following identification based on cultural and molecular characteristics, isolates of different origin were characterized and their cultural, ecological, pathogenic, and antagonistic features were compared. This study has shown that L. corni-maris, originally isolated as an apple fruit pathogen and one as an antagonist of M. polystroma, have similar features in vitro and in vivo. The isolates vary widely in growth rate, cultural morphology, crystal production, intensity of dark zone and co-antagonism with two Monilinia species but can also function as apple fruit pathogens and inhibit and replace different Monilinia spp. on apple fruit. These findings reinforce their dual nature in different host-pathogen interactions. The data from this study are biologically intriguing and practically relevant, and have translative potential to exploit L. corni-maris as a model system for developing strategies to block brown rot of apple and to pinpoint virulence factors in the fungus for yellow rot management.
T2  - Mycological Progress
T2  - Mycological ProgressMycol Progress
T1  - The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp
IS  - 11
SP  - 91
VL  - 21
DO  - 10.1007/s11557-022-01841-w
ER  - 

@article{
author = "Vasić, Miljan and Vico, Ivana and Jurick, Wayne M. and Duduk, Bojan and Duduk, Nataša",
year = "2022",
abstract = "Lambertella corni-maris was isolated from a dark brown lesion on apple fruit cv. Golden Delicious collected from an orchard, and from stored apple fruit cv. Idared previously colonized with Monilia polystroma. The contrasting origin of the isolates led us to hypothesize that this fungus behaves differently in various contexts, which triggered the investigation of L. corni-maris as a pathogen and antagonist. Following identification based on cultural and molecular characteristics, isolates of different origin were characterized and their cultural, ecological, pathogenic, and antagonistic features were compared. This study has shown that L. corni-maris, originally isolated as an apple fruit pathogen and one as an antagonist of M. polystroma, have similar features in vitro and in vivo. The isolates vary widely in growth rate, cultural morphology, crystal production, intensity of dark zone and co-antagonism with two Monilinia species but can also function as apple fruit pathogens and inhibit and replace different Monilinia spp. on apple fruit. These findings reinforce their dual nature in different host-pathogen interactions. The data from this study are biologically intriguing and practically relevant, and have translative potential to exploit L. corni-maris as a model system for developing strategies to block brown rot of apple and to pinpoint virulence factors in the fungus for yellow rot management.",
journal = "Mycological Progress, Mycological ProgressMycol Progress",
title = "The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp",
number = "11",
pages = "91",
volume = "21",
doi = "10.1007/s11557-022-01841-w"
}

Vasić, M., Vico, I., Jurick, W. M., Duduk, B.,& Duduk, N.. (2022). The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp. in Mycological Progress, 21(11), 91.
https://doi.org/10.1007/s11557-022-01841-w

Vasić M, Vico I, Jurick WM, Duduk B, Duduk N. The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp. in Mycological Progress. 2022;21(11):91.
doi:10.1007/s11557-022-01841-w .

Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., Duduk, Bojan, Duduk, Nataša, "The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp" in Mycological Progress, 21, no. 11 (2022):91,
https://doi.org/10.1007/s11557-022-01841-w . .

TY  - JOUR
AU  - Vasić, Miljan
AU  - Vico, Ivana
AU  - Jurick, Wayne M., II
AU  - Duduk, Nataša
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4741
AB  - Brown rot, caused by Monilinia spp., is an economically important pre and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia
EP  - 369
IS  - 2
SP  - 359
VL  - 102
DO  - 10.1094/PDIS-06-17-0867-RE
ER  - 

@article{
author = "Vasić, Miljan and Vico, Ivana and Jurick, Wayne M., II and Duduk, Nataša",
year = "2018",
abstract = "Brown rot, caused by Monilinia spp., is an economically important pre and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia",
pages = "369-359",
number = "2",
volume = "102",
doi = "10.1094/PDIS-06-17-0867-RE"
}

Vasić, M., Vico, I., Jurick, W. M., I.,& Duduk, N.. (2018). Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 102(2), 359-369.
https://doi.org/10.1094/PDIS-06-17-0867-RE

Vasić M, Vico I, Jurick WMI, Duduk N. Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia. in Plant Disease. 2018;102(2):359-369.
doi:10.1094/PDIS-06-17-0867-RE .

Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., II, Duduk, Nataša, "Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia" in Plant Disease, 102, no. 2 (2018):359-369,
https://doi.org/10.1094/PDIS-06-17-0867-RE . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Lazarević, Marina
AU  - Žebeljan, Aleksandra
AU  - Vasić, Miljan
AU  - Vico, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4452
AB  - Onion bulbs (Allium cepa L.) can be stored for up to 12months to ensure their availability all year, but one of the limiting factors is decay caused by blue mould fungi. Postharvest development of blue mould is not only economically important, but also poses a health hazard due to mycotoxin contamination. Onion bulbs of different yellow and red cultivars with blue mould symptoms were collected from three storage facilities in Serbia. Pathogenic isolates were identified and characterized based on their molecular (partial -tubulin gene), morphological (micro- and macromorphology, and colony growth) and biochemical properties (analyses of indole metabolites via Ehrlich test). Three Penicillium species were identified: P.polonicum, P.glabrum and P.expansum. Virulence on inoculated onion bulbs varied significantly within isolates of P.glabrum, but the most virulent isolate of P.polonicum and P.glabrum did not differ, nor did they differ from an isolate of P.expansum. Variability in virulence of individual P.glabrum isolates corresponded to differences in their molecular and macromorphological characters. P.glabrum and P.expansum were identified for the first time as causal agents of onion bulb decay in Serbia. Data from this investigation provide insight into diversity of economically important and possibly toxigenic blue mould fungi which brings attention to their presence in storage facilities and therefore the necessity of the application of prevention measures.
PB  - Wiley, Hoboken
T2  - Journal of Phytopathology
T1  - Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum
EP  - 669
IS  - 10
SP  - 662
VL  - 165
DO  - 10.1111/jph.12605
ER  - 

@article{
author = "Duduk, Nataša and Lazarević, Marina and Žebeljan, Aleksandra and Vasić, Miljan and Vico, Ivana",
year = "2017",
abstract = "Onion bulbs (Allium cepa L.) can be stored for up to 12months to ensure their availability all year, but one of the limiting factors is decay caused by blue mould fungi. Postharvest development of blue mould is not only economically important, but also poses a health hazard due to mycotoxin contamination. Onion bulbs of different yellow and red cultivars with blue mould symptoms were collected from three storage facilities in Serbia. Pathogenic isolates were identified and characterized based on their molecular (partial -tubulin gene), morphological (micro- and macromorphology, and colony growth) and biochemical properties (analyses of indole metabolites via Ehrlich test). Three Penicillium species were identified: P.polonicum, P.glabrum and P.expansum. Virulence on inoculated onion bulbs varied significantly within isolates of P.glabrum, but the most virulent isolate of P.polonicum and P.glabrum did not differ, nor did they differ from an isolate of P.expansum. Variability in virulence of individual P.glabrum isolates corresponded to differences in their molecular and macromorphological characters. P.glabrum and P.expansum were identified for the first time as causal agents of onion bulb decay in Serbia. Data from this investigation provide insight into diversity of economically important and possibly toxigenic blue mould fungi which brings attention to their presence in storage facilities and therefore the necessity of the application of prevention measures.",
publisher = "Wiley, Hoboken",
journal = "Journal of Phytopathology",
title = "Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum",
pages = "669-662",
number = "10",
volume = "165",
doi = "10.1111/jph.12605"
}

Duduk, N., Lazarević, M., Žebeljan, A., Vasić, M.,& Vico, I.. (2017). Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum. in Journal of Phytopathology
Wiley, Hoboken., 165(10), 662-669.
https://doi.org/10.1111/jph.12605

Duduk N, Lazarević M, Žebeljan A, Vasić M, Vico I. Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum. in Journal of Phytopathology. 2017;165(10):662-669.
doi:10.1111/jph.12605 .

Duduk, Nataša, Lazarević, Marina, Žebeljan, Aleksandra, Vasić, Miljan, Vico, Ivana, "Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum" in Journal of Phytopathology, 165, no. 10 (2017):662-669,
https://doi.org/10.1111/jph.12605 . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Marković, Tatjana
AU  - Vasić, Miljan
AU  - Duduk, Bojan
AU  - Vico, Ivana
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3825
AB  - The antifungal effects of thyme, cinnamon bark and clove bud essential oils (EOs) were investigated in vitro on Colletotrichum acutatum mycelial growth, conidial germination, appressoria formation, and in vivo on strawberry fruit disease incidence. All tested EOs, incorporated in potato-dextrose agar, inhibitedC. acutatum mycelial growth, and had a fungistatic effect at concentration 667 mu l/l of medium. Volatiles of cinnamon bark, thyme and clove bud EOs completely prevented conidial germination at the lowest concentrations of 1.53, 15.3 and 76.5 mu l/l of air, respectively, and disabled appressoria formation at concentration of 1.53 mu l/l of air. On inoculated strawberry fruit, thyme and cinnamon bark EO volatiles reduced anthracnose incidence at concentrations above 15.3 and 76.5 mu l/l of air, respectively. GC-FID and GC-MS analysis showed that major components of thyme EO were p-cymene, thymol, alpha-terpineol, carvacrol; cinnamon bark EO: trans- cinnameldehyde, trans-cinnamyl acetate; clove bud EO: eugenol and beta-caryophyllene. Our results suggest thatvolatiles of thyme and cinnamon bark EOs are effective against C. acutatum both in vitro and in vivo.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose
EP  - 537
IS  - 3
SP  - 529
VL  - 18
DO  - 10.1080/0972060X.2015.1004120
ER  - 

@article{
author = "Duduk, Nataša and Marković, Tatjana and Vasić, Miljan and Duduk, Bojan and Vico, Ivana and Obradović, Aleksa",
year = "2015",
abstract = "The antifungal effects of thyme, cinnamon bark and clove bud essential oils (EOs) were investigated in vitro on Colletotrichum acutatum mycelial growth, conidial germination, appressoria formation, and in vivo on strawberry fruit disease incidence. All tested EOs, incorporated in potato-dextrose agar, inhibitedC. acutatum mycelial growth, and had a fungistatic effect at concentration 667 mu l/l of medium. Volatiles of cinnamon bark, thyme and clove bud EOs completely prevented conidial germination at the lowest concentrations of 1.53, 15.3 and 76.5 mu l/l of air, respectively, and disabled appressoria formation at concentration of 1.53 mu l/l of air. On inoculated strawberry fruit, thyme and cinnamon bark EO volatiles reduced anthracnose incidence at concentrations above 15.3 and 76.5 mu l/l of air, respectively. GC-FID and GC-MS analysis showed that major components of thyme EO were p-cymene, thymol, alpha-terpineol, carvacrol; cinnamon bark EO: trans- cinnameldehyde, trans-cinnamyl acetate; clove bud EO: eugenol and beta-caryophyllene. Our results suggest thatvolatiles of thyme and cinnamon bark EOs are effective against C. acutatum both in vitro and in vivo.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose",
pages = "537-529",
number = "3",
volume = "18",
doi = "10.1080/0972060X.2015.1004120"
}

Duduk, N., Marković, T., Vasić, M., Duduk, B., Vico, I.,& Obradović, A.. (2015). Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 18(3), 529-537.
https://doi.org/10.1080/0972060X.2015.1004120

Duduk N, Marković T, Vasić M, Duduk B, Vico I, Obradović A. Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose. in Journal of Essential Oil Bearing Plants. 2015;18(3):529-537.
doi:10.1080/0972060X.2015.1004120 .

Duduk, Nataša, Marković, Tatjana, Vasić, Miljan, Duduk, Bojan, Vico, Ivana, Obradović, Aleksa, "Antifungal Activity of Three Essential Oils against Colletotrichum acutatum, the Causal Agent of Strawberry Anthracnose" in Journal of Essential Oil Bearing Plants, 18, no. 3 (2015):529-537,
https://doi.org/10.1080/0972060X.2015.1004120 . .

TY  - JOUR
AU  - Lazarević, Marina
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Vico, Ivana
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3606
AB  - Penicillium polonicum is an economically important fungal species with a wide host range which includes cereals, peanuts, onions, dried meats, citrus, apple, and yam tubers. Onion bulbs cv. Meranto with blue mold symptoms have been collected, in a storage facility in Stara Pazova during 2013. Fungal isolates were obtained from decayed bulbs and their pathogenicity was proven after wound inoculation of healthy onion bulbs. On differential media the isolates formed velutinous, green blue colonies with a white margin and a characteristic odor. The isolates grew at 5 C while no growth was observed at 37 C. Isolates produced cyclopiazonic acid and other alkaloids. Conidiophores of isolates were terverticillate, stipes were septate with smooth to finely roughened walls, and phialides were ampulliform. Conidia were globose to subglobose, smooth-walled, and borne in columns. The nucleotide sequences of amplified products (~800 bp) for partial β-tubulin gene showed in MegaBLAST search analysis a 99% similarity with several sequences of P. polonicum. Based on morphological and molecular features isolates obtained from stored onion were identified as P. polonicum.
AB  - Penicillium polonicum je ekonomski važna gljiva sa širokim krugom domaćina, uključujući žitarice, kikiriki, lukove, sušeno meso, citruse, jabuke i krtole jama. U januaru 2013. godine, u skladištu na teritoriji Stare Pazove prikupljeni su uzorci lukovica crnog luka cv. Meranto sa karakterističnim simptomima plave truleži. Iz obolelih lukovica dobijeni su izolati gljive čija je patogenost potvrđena inokulacijom zdravih lukovica. Na diferencijalnim podlogama izolati su formirali dobro razvijene, guste kolonije, plavozelene boje sa belim obodom, karakterističnog mirisa. Izolati nisu rasli na 37oC, dok je porast zabeležen na 5oC. Utvrđeno je da su izolati stvarali ciklopiazonsku kiselinu i druge alkaloide. Konidiofore izolata su bile terverticilate, stipe septirane, tankih i glatkih zidova, a fijalide cilindrične. Konidije su bile loptaste do elipsoidne, glatke i formirale su se u dugim lancima. Sekvence dobijenih amplikona veličine oko 800 bp dela gena za β-tubulin su u MegaBLAST analizi pokazali najvišu sličnost od 99% sa sekvencama istog regiona vrste P. polonicum. Na osnovu morfoloških i molekularnih osobina kao prouzrokovač propadanja lukovica crnog luka u skladištu identifikovana je gljiva P. polonicum.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Penicillium polonicum: Causal agent of onion bulb decay in storage
T1  - Penicillium polonicum - prouzrokovač propadanja lukovica crnog luka u skladištu
EP  - 110
IS  - 3
SP  - 105
VL  - 65
DO  - 10.5937/zasbilj1403105L
ER  - 

@article{
author = "Lazarević, Marina and Duduk, Nataša and Vasić, Miljan and Vico, Ivana",
year = "2014",
abstract = "Penicillium polonicum is an economically important fungal species with a wide host range which includes cereals, peanuts, onions, dried meats, citrus, apple, and yam tubers. Onion bulbs cv. Meranto with blue mold symptoms have been collected, in a storage facility in Stara Pazova during 2013. Fungal isolates were obtained from decayed bulbs and their pathogenicity was proven after wound inoculation of healthy onion bulbs. On differential media the isolates formed velutinous, green blue colonies with a white margin and a characteristic odor. The isolates grew at 5 C while no growth was observed at 37 C. Isolates produced cyclopiazonic acid and other alkaloids. Conidiophores of isolates were terverticillate, stipes were septate with smooth to finely roughened walls, and phialides were ampulliform. Conidia were globose to subglobose, smooth-walled, and borne in columns. The nucleotide sequences of amplified products (~800 bp) for partial β-tubulin gene showed in MegaBLAST search analysis a 99% similarity with several sequences of P. polonicum. Based on morphological and molecular features isolates obtained from stored onion were identified as P. polonicum., Penicillium polonicum je ekonomski važna gljiva sa širokim krugom domaćina, uključujući žitarice, kikiriki, lukove, sušeno meso, citruse, jabuke i krtole jama. U januaru 2013. godine, u skladištu na teritoriji Stare Pazove prikupljeni su uzorci lukovica crnog luka cv. Meranto sa karakterističnim simptomima plave truleži. Iz obolelih lukovica dobijeni su izolati gljive čija je patogenost potvrđena inokulacijom zdravih lukovica. Na diferencijalnim podlogama izolati su formirali dobro razvijene, guste kolonije, plavozelene boje sa belim obodom, karakterističnog mirisa. Izolati nisu rasli na 37oC, dok je porast zabeležen na 5oC. Utvrđeno je da su izolati stvarali ciklopiazonsku kiselinu i druge alkaloide. Konidiofore izolata su bile terverticilate, stipe septirane, tankih i glatkih zidova, a fijalide cilindrične. Konidije su bile loptaste do elipsoidne, glatke i formirale su se u dugim lancima. Sekvence dobijenih amplikona veličine oko 800 bp dela gena za β-tubulin su u MegaBLAST analizi pokazali najvišu sličnost od 99% sa sekvencama istog regiona vrste P. polonicum. Na osnovu morfoloških i molekularnih osobina kao prouzrokovač propadanja lukovica crnog luka u skladištu identifikovana je gljiva P. polonicum.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Penicillium polonicum: Causal agent of onion bulb decay in storage, Penicillium polonicum - prouzrokovač propadanja lukovica crnog luka u skladištu",
pages = "110-105",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403105L"
}

Lazarević, M., Duduk, N., Vasić, M.,& Vico, I.. (2014). Penicillium polonicum: Causal agent of onion bulb decay in storage. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 65(3), 105-110.
https://doi.org/10.5937/zasbilj1403105L

Lazarević M, Duduk N, Vasić M, Vico I. Penicillium polonicum: Causal agent of onion bulb decay in storage. in Zaštita bilja. 2014;65(3):105-110.
doi:10.5937/zasbilj1403105L .

Lazarević, Marina, Duduk, Nataša, Vasić, Miljan, Vico, Ivana, "Penicillium polonicum: Causal agent of onion bulb decay in storage" in Zaštita bilja, 65, no. 3 (2014):105-110,
https://doi.org/10.5937/zasbilj1403105L . .

TY  - JOUR
AU  - Vico, Ivana
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Nikolić, Milica
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3561
AB  - Penicillium expansum (Link) Thom. is one of the most important postharvest pathogens of apple fruit worldwide. It causes blue mold, a decay that can lead to significant economic losses during storage, which can also impact fruit destined for processing due to the production of carcinogenic mycotoxin patulin. Apple fruit cvs. Idared, Golden Delicious and Braeburn with blue mold symptoms were collected from five storage facilities in Serbia and nine fungal isolates were obtained. Pathogenicity of the isolates was tested and proven by artificial inoculation of healthy apples cv. Idared. In order to identify the causal agents of decay, morphological and molecular methods were used. Colony morphology and microscopic features were observed on differential media, and isolates were tested for the production of cyclopiazonic acid. Molecular analysis included PCR amplification with species specific primers for P. expansum based on polygalacturonase gene (Pepg1), universal primers for internal transcribed spacer rDNA region and primers based on β-tubulin gene. All isolates formed compact blue green colonies with characteristic earthy odor. Conidiophores were terverticillate with smooth septate stipes and conidia were smooth, globose to subglobose, born in colums. The average size of conidia was 3.38 +- 0.49 (SD) x 3 +- 0.36 (SD) μm. Using species specific primers PEF/PER the texpected amplicons of ~404 bp were obtained in all nine tested isolates and PCR conducted with the Bt-LEVUp4/ Bt-LEV-Lo1 and universal ITS1/ITS4 primer pairs generated amplicons of the expected sizes of ~800 bp and ~600 bp, respectively. MegaBlast analyses of the 2X consensus of nucleotide sequences of the isolate JP1 partial β-tubulin gene and ITS region showed 99-100% and 100% similarity with several P. expansum sequences of corresponding regions of this species deposited in GenBank. Based on morphological and molecular features, the isolates obtained from decayed apple fruit collected in several storage facilities in Serbia were identified as P. expansum.
AB  - Penicillium expansum (Link) Thom. je jedan od najznačajnijih prouzrokovača truleži plodova jabučastog voća u toku čuvanja koji se odlikuje proizvodnjom mikotoksina patulina. U ovom radu je, na osnovu morfoloških i molekularnih osobina, identifikovana vrsta P. expansum kao prouzrokovač truleži plodova jabuke u skladištu. Plodovi jabuke sorti Ajdared, Zlatni delišes i Breburn sa simptomima truleži prikupljene su u skladištima u lokalitetima Pudarci, Suvodol, Čelarevo, Bela Crkva i Svilajnac. Na prikupljenim plodovima uočavale su se svetlosmeđe, meke i udubljene pege u okviru kojih je bila prisutna zelenoplava sporulacija gljive. Izolacija je obavljena standardnim postupkom na podlogu od krompir-dekstroznog agara (KDA), a patogenost izolata proverena je inokulacijom zdravih plodova jabuke. Morfološke odlike kolonija i reproduktivnih struktura izolata praćene su na dijagnostičkim hranljivim podlogama (MEA, CYA i YES). Molekularna identifikacija izolata obavljena je korišćenjem specifičnih prajmera za vrstu P. expansum zasnovanih na genu za poligalakturonazu (Pepg1), univerzalnih prajmera za ITS region i prajmera zasnovanih na genu za β-tubulin. Iz jabuke sa simptomima plave truleži dobijeno je devet izolata čija je patogenost potvrdjena veštačkim inokulacijama zdravih plodova jabuke. Ispitivani izolati su formirali kompaktne zelenoplave kolonije karakterističnog mirisa. Svi izolati su rasli na 5oC, a porasta nije bilo na 37oC. Konidije prosečne veličine 3,38 x 3 μm, bile su okruglaste do ovalne, formirane u dugim nizovima. Konidiofore su bile terverticillata, asimetričnog grananja sa glatkim stipama. Kod svih ispitivanih izolata amplikoni očekivane veličine oko 404 bp dobijeni su korišćenjem specifičnih prajmera za P. expansum PEF/PER, dok su primenom ITS1-ITS4 prajmera amplifikovani produkti veličine oko 600 bp, a primenom prajmera za deo β-tubulin gena, produkti veličine oko 800 bp. MegaBlast analizom dobijenih sekvenci ITS regiona i dela β-tubulin gena izabranog izolata JP1 utvrđena je 100%, odnosno 99-100% sličnost sa sekvencama odgovarajućih regiona vrste P. expansum deponovanim u NCBI bazi podataka. Na osnovu morfoloških i molekularnih osobina dobijenih izolata utvrđeno je da prouzrokovači truleži plodova jabuke u nekoliko skladišta u Srbiji pripadaju vrsti P. expansum.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit
T1  - Identifikacija Penicillium expansum prouzrokovača truleži plodova jabuke u skladištu
EP  - 266
IS  - 4
SP  - 257
VL  - 29
DO  - 10.2298/PIF1404257V
ER  - 

@article{
author = "Vico, Ivana and Duduk, Nataša and Vasić, Miljan and Nikolić, Milica",
year = "2014",
abstract = "Penicillium expansum (Link) Thom. is one of the most important postharvest pathogens of apple fruit worldwide. It causes blue mold, a decay that can lead to significant economic losses during storage, which can also impact fruit destined for processing due to the production of carcinogenic mycotoxin patulin. Apple fruit cvs. Idared, Golden Delicious and Braeburn with blue mold symptoms were collected from five storage facilities in Serbia and nine fungal isolates were obtained. Pathogenicity of the isolates was tested and proven by artificial inoculation of healthy apples cv. Idared. In order to identify the causal agents of decay, morphological and molecular methods were used. Colony morphology and microscopic features were observed on differential media, and isolates were tested for the production of cyclopiazonic acid. Molecular analysis included PCR amplification with species specific primers for P. expansum based on polygalacturonase gene (Pepg1), universal primers for internal transcribed spacer rDNA region and primers based on β-tubulin gene. All isolates formed compact blue green colonies with characteristic earthy odor. Conidiophores were terverticillate with smooth septate stipes and conidia were smooth, globose to subglobose, born in colums. The average size of conidia was 3.38 +- 0.49 (SD) x 3 +- 0.36 (SD) μm. Using species specific primers PEF/PER the texpected amplicons of ~404 bp were obtained in all nine tested isolates and PCR conducted with the Bt-LEVUp4/ Bt-LEV-Lo1 and universal ITS1/ITS4 primer pairs generated amplicons of the expected sizes of ~800 bp and ~600 bp, respectively. MegaBlast analyses of the 2X consensus of nucleotide sequences of the isolate JP1 partial β-tubulin gene and ITS region showed 99-100% and 100% similarity with several P. expansum sequences of corresponding regions of this species deposited in GenBank. Based on morphological and molecular features, the isolates obtained from decayed apple fruit collected in several storage facilities in Serbia were identified as P. expansum., Penicillium expansum (Link) Thom. je jedan od najznačajnijih prouzrokovača truleži plodova jabučastog voća u toku čuvanja koji se odlikuje proizvodnjom mikotoksina patulina. U ovom radu je, na osnovu morfoloških i molekularnih osobina, identifikovana vrsta P. expansum kao prouzrokovač truleži plodova jabuke u skladištu. Plodovi jabuke sorti Ajdared, Zlatni delišes i Breburn sa simptomima truleži prikupljene su u skladištima u lokalitetima Pudarci, Suvodol, Čelarevo, Bela Crkva i Svilajnac. Na prikupljenim plodovima uočavale su se svetlosmeđe, meke i udubljene pege u okviru kojih je bila prisutna zelenoplava sporulacija gljive. Izolacija je obavljena standardnim postupkom na podlogu od krompir-dekstroznog agara (KDA), a patogenost izolata proverena je inokulacijom zdravih plodova jabuke. Morfološke odlike kolonija i reproduktivnih struktura izolata praćene su na dijagnostičkim hranljivim podlogama (MEA, CYA i YES). Molekularna identifikacija izolata obavljena je korišćenjem specifičnih prajmera za vrstu P. expansum zasnovanih na genu za poligalakturonazu (Pepg1), univerzalnih prajmera za ITS region i prajmera zasnovanih na genu za β-tubulin. Iz jabuke sa simptomima plave truleži dobijeno je devet izolata čija je patogenost potvrdjena veštačkim inokulacijama zdravih plodova jabuke. Ispitivani izolati su formirali kompaktne zelenoplave kolonije karakterističnog mirisa. Svi izolati su rasli na 5oC, a porasta nije bilo na 37oC. Konidije prosečne veličine 3,38 x 3 μm, bile su okruglaste do ovalne, formirane u dugim nizovima. Konidiofore su bile terverticillata, asimetričnog grananja sa glatkim stipama. Kod svih ispitivanih izolata amplikoni očekivane veličine oko 404 bp dobijeni su korišćenjem specifičnih prajmera za P. expansum PEF/PER, dok su primenom ITS1-ITS4 prajmera amplifikovani produkti veličine oko 600 bp, a primenom prajmera za deo β-tubulin gena, produkti veličine oko 800 bp. MegaBlast analizom dobijenih sekvenci ITS regiona i dela β-tubulin gena izabranog izolata JP1 utvrđena je 100%, odnosno 99-100% sličnost sa sekvencama odgovarajućih regiona vrste P. expansum deponovanim u NCBI bazi podataka. Na osnovu morfoloških i molekularnih osobina dobijenih izolata utvrđeno je da prouzrokovači truleži plodova jabuke u nekoliko skladišta u Srbiji pripadaju vrsti P. expansum.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit, Identifikacija Penicillium expansum prouzrokovača truleži plodova jabuke u skladištu",
pages = "266-257",
number = "4",
volume = "29",
doi = "10.2298/PIF1404257V"
}

Vico, I., Duduk, N., Vasić, M.,& Nikolić, M.. (2014). Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 29(4), 257-266.
https://doi.org/10.2298/PIF1404257V

Vico I, Duduk N, Vasić M, Nikolić M. Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit. in Pesticidi i fitomedicina. 2014;29(4):257-266.
doi:10.2298/PIF1404257V .

Vico, Ivana, Duduk, Nataša, Vasić, Miljan, Nikolić, Milica, "Identification of Penicillium expansum causing postharvest blue mold decay of apple fruit" in Pesticidi i fitomedicina, 29, no. 4 (2014):257-266,
https://doi.org/10.2298/PIF1404257V . .

TY  - JOUR
AU  - Vico, Ivana
AU  - Gaskins, Verneta L.
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Yu, J.J.
AU  - Peter, K.A.
AU  - Jurick, Wayne M., II
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3403
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia
EP  - 1430
IS  - 10
SP  - 1430
VL  - 98
DO  - 10.1094/PDIS-02-14-0179-PDN
ER  - 

@article{
author = "Vico, Ivana and Gaskins, Verneta L. and Duduk, Nataša and Vasić, Miljan and Yu, J.J. and Peter, K.A. and Jurick, Wayne M., II",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia",
pages = "1430-1430",
number = "10",
volume = "98",
doi = "10.1094/PDIS-02-14-0179-PDN"
}

Vico, I., Gaskins, V. L., Duduk, N., Vasić, M., Yu, J.J., Peter, K.A.,& Jurick, W. M., I.. (2014). First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(10), 1430-1430.
https://doi.org/10.1094/PDIS-02-14-0179-PDN

Vico I, Gaskins VL, Duduk N, Vasić M, Yu J, Peter K, Jurick WMI. First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia. in Plant Disease. 2014;98(10):1430-1430.
doi:10.1094/PDIS-02-14-0179-PDN .

Vico, Ivana, Gaskins, Verneta L., Duduk, Nataša, Vasić, Miljan, Yu, J.J., Peter, K.A., Jurick, Wayne M., II, "First Report of Penicillium crustosum Causing Blue Mold on Stored Apple Fruit in Serbia" in Plant Disease, 98, no. 10 (2014):1430-1430,
https://doi.org/10.1094/PDIS-02-14-0179-PDN . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Vasić, Miljan
AU  - Vico, Ivana
AU  - Duduk, Bojan
AU  - Marković, Tatjana
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3238
AB  - Plants belonging to Brassicaceae family accumulate secondary metabolites, glucosinolates, as their constituent compounds. The amount of glucosinolates in brassica plants depend on growth stage, plant part, ecological conditions and agricultural practices. As a reaction to wounding and injury of plant tissue glucosinolates undergo enzymatic hydrolysis with myrosinase and volatile isothiocyanates are released. These volatiles have been long known for their fungicidal, bactericidal, nematocidal and alelopatic properties. Antifungal potencial of isothiocyanates originating from Brassicaceae plants has been confirmed towards many plant pathogenic fungi. Glucosinolates and the products of their hydrolysis are natural products which are considered to be fully biodegradable and non-toxic, making them eligible contenders for organic and integrated pest management.
AB  - Biljke iz familije Brassicaceae konstitutivno akumuliraju značajne količine sekundarnih metabolita, glukozinolata. Sadržaj glukozinolata u kupusima zavisi od fenofaze razvoja, biljnog dela, ekoloških i agrotehničkih faktora. Usled povreda i oštećenja biljnog tkiva pod uticajem enzima mirozinaze dolazi do hidrolitičkog razlaganja glukozinolata, pri čemu se oslobađaju isparljivi izotiocijanati. Ova jedinjenja su odavno poznati po svom fungicidnom, baktericidnom, nematocidnom i alelopatskom svojstvu. Antifungalni potencijal izotiocijanata biljaka iz familije Brassicaceae potvrđen je prema mnogim fitopatogenim gljivama. Glukozinolati i njihovi hidrolitički produkti izotiocijanati predstavljaju prirodna, biodegradiblna i netoksična jedinjenja biljaka sa velikim antifungalnim potencijalom, što ih čini pogodnim alternativnim merama kontrole patogena u organskoj i integralnoj zaštiti bilja.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Glucosinolates: Plant derived antifungal compounds
T1  - Glukozinolati - prirodna antifungalna jedinjenja
EP  - 369
IS  - 3
SP  - 362
VL  - 41
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3238
ER  - 

@article{
author = "Duduk, Nataša and Vasić, Miljan and Vico, Ivana and Duduk, Bojan and Marković, Tatjana",
year = "2013",
abstract = "Plants belonging to Brassicaceae family accumulate secondary metabolites, glucosinolates, as their constituent compounds. The amount of glucosinolates in brassica plants depend on growth stage, plant part, ecological conditions and agricultural practices. As a reaction to wounding and injury of plant tissue glucosinolates undergo enzymatic hydrolysis with myrosinase and volatile isothiocyanates are released. These volatiles have been long known for their fungicidal, bactericidal, nematocidal and alelopatic properties. Antifungal potencial of isothiocyanates originating from Brassicaceae plants has been confirmed towards many plant pathogenic fungi. Glucosinolates and the products of their hydrolysis are natural products which are considered to be fully biodegradable and non-toxic, making them eligible contenders for organic and integrated pest management., Biljke iz familije Brassicaceae konstitutivno akumuliraju značajne količine sekundarnih metabolita, glukozinolata. Sadržaj glukozinolata u kupusima zavisi od fenofaze razvoja, biljnog dela, ekoloških i agrotehničkih faktora. Usled povreda i oštećenja biljnog tkiva pod uticajem enzima mirozinaze dolazi do hidrolitičkog razlaganja glukozinolata, pri čemu se oslobađaju isparljivi izotiocijanati. Ova jedinjenja su odavno poznati po svom fungicidnom, baktericidnom, nematocidnom i alelopatskom svojstvu. Antifungalni potencijal izotiocijanata biljaka iz familije Brassicaceae potvrđen je prema mnogim fitopatogenim gljivama. Glukozinolati i njihovi hidrolitički produkti izotiocijanati predstavljaju prirodna, biodegradiblna i netoksična jedinjenja biljaka sa velikim antifungalnim potencijalom, što ih čini pogodnim alternativnim merama kontrole patogena u organskoj i integralnoj zaštiti bilja.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Glucosinolates: Plant derived antifungal compounds, Glukozinolati - prirodna antifungalna jedinjenja",
pages = "369-362",
number = "3",
volume = "41",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3238"
}

Duduk, N., Vasić, M., Vico, I., Duduk, B.,& Marković, T.. (2013). Glucosinolates: Plant derived antifungal compounds. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 41(3), 362-369.
https://hdl.handle.net/21.15107/rcub_agrospace_3238

Duduk N, Vasić M, Vico I, Duduk B, Marković T. Glucosinolates: Plant derived antifungal compounds. in Biljni lekar. 2013;41(3):362-369.
https://hdl.handle.net/21.15107/rcub_agrospace_3238 .

Duduk, Nataša, Vasić, Miljan, Vico, Ivana, Duduk, Bojan, Marković, Tatjana, "Glucosinolates: Plant derived antifungal compounds" in Biljni lekar, 41, no. 3 (2013):362-369,
https://hdl.handle.net/21.15107/rcub_agrospace_3238 .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Vico, Ivana
AU  - Vasić, Miljan
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2912
AB  - Volatile compounds including fundamental aroma constituents are naturally produced by plants as secondary metabolites and emitted into the environment. Fatty acids are the major precursors of volatile compounds responsible for aroma. They are transformed into aldehydes, alcohols, acids and esters trough the oxidative degradation pathway. The two most important products that have been studied for antifungal activity from this group of compounds are aldehydes, hexanal and trans-2-hexenal. Since there is increasing evidence based on investigation results that plant-derived compounds exhibit antifungal effects, research is done targeting possible practical applications. In the review antifungal effects of hexanal and related compounds in vitro and in vivo on economically important plant pathogens have been discussed.
AB  - Biljke oslobađaju u gasovitom stanju u spoljašnju sredinu mnoge sekundarne metabolite, među kojima i jedinjenja koja određuju aromu. Glavni prekursori u sintezi ovih jedinjenja su masne kiseline, čijim oksidativnim razlaganjem nastaju aldehidi, alkoholi, kiseline i estri. Među njima, najtoksičniji prema fitopatogenim gljivama su aldehidi, trans-2-heksenal i heksanal. Veliki broj istraživanja usmeren je ispitivanju antifungalnog potencijala biljnih komponenti prema značajnim fitopatogenim gljivama i mogućnostima njihove praktične primene. U radu su prikazani rezultati antifungalnog delovanja heksanala i srodnih jedinjenja in vitro i in vivo prema ekonomski značajnim prouzrokovačima biljnih bolesti.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Antifungal potential of hexanal and related compounds
T1  - Antifungalni potencijal heksanala i srodnih jedinjenja
EP  - 508
IS  - 6
SP  - 502
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2912
ER  - 

@article{
author = "Duduk, Nataša and Vico, Ivana and Vasić, Miljan",
year = "2012",
abstract = "Volatile compounds including fundamental aroma constituents are naturally produced by plants as secondary metabolites and emitted into the environment. Fatty acids are the major precursors of volatile compounds responsible for aroma. They are transformed into aldehydes, alcohols, acids and esters trough the oxidative degradation pathway. The two most important products that have been studied for antifungal activity from this group of compounds are aldehydes, hexanal and trans-2-hexenal. Since there is increasing evidence based on investigation results that plant-derived compounds exhibit antifungal effects, research is done targeting possible practical applications. In the review antifungal effects of hexanal and related compounds in vitro and in vivo on economically important plant pathogens have been discussed., Biljke oslobađaju u gasovitom stanju u spoljašnju sredinu mnoge sekundarne metabolite, među kojima i jedinjenja koja određuju aromu. Glavni prekursori u sintezi ovih jedinjenja su masne kiseline, čijim oksidativnim razlaganjem nastaju aldehidi, alkoholi, kiseline i estri. Među njima, najtoksičniji prema fitopatogenim gljivama su aldehidi, trans-2-heksenal i heksanal. Veliki broj istraživanja usmeren je ispitivanju antifungalnog potencijala biljnih komponenti prema značajnim fitopatogenim gljivama i mogućnostima njihove praktične primene. U radu su prikazani rezultati antifungalnog delovanja heksanala i srodnih jedinjenja in vitro i in vivo prema ekonomski značajnim prouzrokovačima biljnih bolesti.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Antifungal potential of hexanal and related compounds, Antifungalni potencijal heksanala i srodnih jedinjenja",
pages = "508-502",
number = "6",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2912"
}

Duduk, N., Vico, I.,& Vasić, M.. (2012). Antifungal potential of hexanal and related compounds. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 40(6), 502-508.
https://hdl.handle.net/21.15107/rcub_agrospace_2912

Duduk N, Vico I, Vasić M. Antifungal potential of hexanal and related compounds. in Biljni lekar. 2012;40(6):502-508.
https://hdl.handle.net/21.15107/rcub_agrospace_2912 .

Duduk, Nataša, Vico, Ivana, Vasić, Miljan, "Antifungal potential of hexanal and related compounds" in Biljni lekar, 40, no. 6 (2012):502-508,
https://hdl.handle.net/21.15107/rcub_agrospace_2912 .