TY  - JOUR
AU  - Žebeljan, Aleksandra
AU  - Duduk, Nataša
AU  - Vučković, Nina
AU  - Jurick II, Wayne M.
AU  - Vico, Ivana
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6020
AB  - Blue mold, caused by Penicillium spp., is one of the most economically important postharvest diseases of pome fruits, globally. Pome fruits, in particular apple, is the most widely grown pome fruit in Serbia, and the distribution of Penicillium spp. responsible for postharvest decay is unknown. A two-year survey was conducted in 2014 and 2015, where four pome fruits (apple, pear, quince, and medlar) with blue mold symptoms were collected from 20 storage locations throughout Serbia. Detailed morphological characterization, analysis of virulence in three apple cultivars, and multilocus phylogeny revealed three main Penicillium spp. in order of abundance: P. expansum, P. crustosum, and P. solitum. Interestingly, P. expansum split into two distinct clades with strong statistical support that coincided with several morphological observations. Findings from this study are significant and showed previously undocumented diversity in blue mold fungi responsible for postharvest decay including the first finding of P. crustosum, and P. solitum as postharvest pathogens of quince and P. crustosum of medlar fruit in the world, and P. expansum of quince in Serbia. Data from this study provide timely information regarding phenotypic, morphological and genotypic plasticity in P. expansum that will impact the design of species-specific detection tools and guide the development of blue mold management strategies.
PB  - MDPI
T2  - J. Fungi
T1  - Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia
IS  - 12
SP  - 1019
VL  - 7
DO  - 10.3390/jof7121019
ER  - 

@article{
author = "Žebeljan, Aleksandra and Duduk, Nataša and Vučković, Nina and Jurick II, Wayne M. and Vico, Ivana",
year = "2021",
abstract = "Blue mold, caused by Penicillium spp., is one of the most economically important postharvest diseases of pome fruits, globally. Pome fruits, in particular apple, is the most widely grown pome fruit in Serbia, and the distribution of Penicillium spp. responsible for postharvest decay is unknown. A two-year survey was conducted in 2014 and 2015, where four pome fruits (apple, pear, quince, and medlar) with blue mold symptoms were collected from 20 storage locations throughout Serbia. Detailed morphological characterization, analysis of virulence in three apple cultivars, and multilocus phylogeny revealed three main Penicillium spp. in order of abundance: P. expansum, P. crustosum, and P. solitum. Interestingly, P. expansum split into two distinct clades with strong statistical support that coincided with several morphological observations. Findings from this study are significant and showed previously undocumented diversity in blue mold fungi responsible for postharvest decay including the first finding of P. crustosum, and P. solitum as postharvest pathogens of quince and P. crustosum of medlar fruit in the world, and P. expansum of quince in Serbia. Data from this study provide timely information regarding phenotypic, morphological and genotypic plasticity in P. expansum that will impact the design of species-specific detection tools and guide the development of blue mold management strategies.",
publisher = "MDPI",
journal = "J. Fungi",
title = "Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia",
number = "12",
pages = "1019",
volume = "7",
doi = "10.3390/jof7121019"
}

Žebeljan, A., Duduk, N., Vučković, N., Jurick II, W. M.,& Vico, I.. (2021). Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia. in J. Fungi
MDPI., 7(12), 1019.
https://doi.org/10.3390/jof7121019

Žebeljan A, Duduk N, Vučković N, Jurick II WM, Vico I. Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia. in J. Fungi. 2021;7(12):1019.
doi:10.3390/jof7121019 .

Žebeljan, Aleksandra, Duduk, Nataša, Vučković, Nina, Jurick II, Wayne M., Vico, Ivana, "Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia" in J. Fungi, 7, no. 12 (2021):1019,
https://doi.org/10.3390/jof7121019 . .

TY  - JOUR
AU  - Žebeljan, Aleksandra
AU  - Vico, Ivana
AU  - Duduk, Nataša
AU  - Ziberna, Bojana
AU  - Krajnc, Andreja Urbanek
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5070
AB  - Penicillium expansum-infected apple fruit resulted in a disrupted balance of primary metabolites and impacted the ascorbate-glutathione cycle. The initial decline in fructose, malic acid, shikimic acid and total ascorbate content (6 hpi) was followed by a significant increase in malic acid (24 hpi) and total glutathione (3 dpi). Significant increase in sucrose was accompanied by a decline in glucose and ascorbate content (5 dpi). Total glutathione and shikimic acid gradually deteriorated with symptom development. A negative correlation was found between sucrose and ascorbate-glutathione pool. Other correlations were strong amongst other metabolites and lesion size indicating that they may be involved or important for apple fruit decay.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Physiological and Molecular Plant Pathology
T1  - Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions
EP  - 174
SP  - 166
VL  - 106
DO  - 10.1016/j.pmpp.2019.02.001
ER  - 

@article{
author = "Žebeljan, Aleksandra and Vico, Ivana and Duduk, Nataša and Ziberna, Bojana and Krajnc, Andreja Urbanek",
year = "2019",
abstract = "Penicillium expansum-infected apple fruit resulted in a disrupted balance of primary metabolites and impacted the ascorbate-glutathione cycle. The initial decline in fructose, malic acid, shikimic acid and total ascorbate content (6 hpi) was followed by a significant increase in malic acid (24 hpi) and total glutathione (3 dpi). Significant increase in sucrose was accompanied by a decline in glucose and ascorbate content (5 dpi). Total glutathione and shikimic acid gradually deteriorated with symptom development. A negative correlation was found between sucrose and ascorbate-glutathione pool. Other correlations were strong amongst other metabolites and lesion size indicating that they may be involved or important for apple fruit decay.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Physiological and Molecular Plant Pathology",
title = "Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions",
pages = "174-166",
volume = "106",
doi = "10.1016/j.pmpp.2019.02.001"
}

Žebeljan, A., Vico, I., Duduk, N., Ziberna, B.,& Krajnc, A. U.. (2019). Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions. in Physiological and Molecular Plant Pathology
Academic Press Ltd- Elsevier Science Ltd, London., 106, 166-174.
https://doi.org/10.1016/j.pmpp.2019.02.001

Žebeljan A, Vico I, Duduk N, Ziberna B, Krajnc AU. Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions. in Physiological and Molecular Plant Pathology. 2019;106:166-174.
doi:10.1016/j.pmpp.2019.02.001 .

Žebeljan, Aleksandra, Vico, Ivana, Duduk, Nataša, Ziberna, Bojana, Krajnc, Andreja Urbanek, "Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions" in Physiological and Molecular Plant Pathology, 106 (2019):166-174,
https://doi.org/10.1016/j.pmpp.2019.02.001 . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Vasić, Miljan M.
AU  - Vučković, Nina R.
AU  - Žebeljan, Aleksandra
AU  - Vico, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4574
AB  - Monilinia spp. are economically important pathogens of pome and stone fruits. Four Monilinia species are present in Serbia - Monilinia fructigena, M. laxa, M. fructicola and Monilia polystroma. As detection and identification of Monilinia species are complex, the aim of this research was to evaluate species-specific primers in PCR in order to standardize fast and reliable molecular methods for differentiation between the four Monilinia species. Isolates of M. fructigena, M. laxa, M. fructicola and M. polystroma from apple fruit and referent isolates from Italy and Japan were used for testing. Specific molecular detection of M. laxa was obtained using ITS1Mlx/ITS4Mlx and Ml-Mfg-F2/Ml-Mfc-R1 primer pairs, and M. fructicola using ITS1Mfcl/ITS4Mfcl and Mfc-F1/Mfc-R1 primer pairs. ITS1Mfgn/ITS4Mfgn and ITS1/Mfg-R2 primer pairs, described as M. fructigena species-specific, amplified M. fructigena and M. polystroma, as well. Specific detection of these two species as well as of all four tested Monilinia species was obtained using the reverse primer MO368-5 with forward primers MO368-8R, Laxa-R2 and MO368-10R in separate or in Multiplex PCR reactions.
AB  - Vrste roda Monilinia su ekonomski znač ajni patogeni jabučastih i koštičavih vrsta voćaka. U Srbiji su prisutne četiri vrste ovog roda: M. fructigena, M. laxa, M. fructicola i Monilia polystroma. Detekcija i identifikacija vrsta roda Monilinia je složena, tako da je cilj ovog rada bio da se ispita i standardizuje brza i efikasna metoda molekularne detekcije korišćenjem različitih prajmera u PCR metodi i da se utvrdi njihova specifičnost i mogućnost korišćenja u identifikaciji Monilinia spp. U radu su korišćeni izolati M. fructigena, M. laxa, M. fructicola i M. polystroma poreklom iz plodova jabuke prikupljenih u Srbiji, kao i referentni izolati iz Italije i Japana. Specifična molekularna detekcija M. laxa postignuta je korišćenjem prajmera ITS1Mlx/ITS4Mlx i Ml-Mfg -F2/Ml-Mfc-R1, a vrste M. fructicola korišćenjem prajmera ITS1Mfcl/ITS4Mfcl i Mfc-F1/Mfc-R1. Prajmeri ITS1Mfgn/ITS4Mfgn i ITS1/Mfg -R2, koji su u literaturi opisani kao specifični za M. fructigena, pored izolata M. fructigena amplifikovali su i DNK izolata M. polystroma. Za razdvajanje ove dve vrste, kao i za razlikovanje sve četiri ispitivane vrste roda Monilinia, najpogodniji su uzvodni prajmer MO368-5 u kombinaciji sa nizvodnim prajmerima MO368-8R, Laxa-R2 i MO368-10R u direktnoj ili Multiplex PCR metodi.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Suitability of different primers for specific molecular detection of Monilinia spp.
T1  - Pogodnost različitih prajmera za specifičnu molekularnu detekciju Monilinia spp.
EP  - 177
IS  - 2
SP  - 167
VL  - 62
DO  - 10.2298/JAS1702167D
ER  - 

@article{
author = "Duduk, Nataša and Vasić, Miljan M. and Vučković, Nina R. and Žebeljan, Aleksandra and Vico, Ivana",
year = "2017",
abstract = "Monilinia spp. are economically important pathogens of pome and stone fruits. Four Monilinia species are present in Serbia - Monilinia fructigena, M. laxa, M. fructicola and Monilia polystroma. As detection and identification of Monilinia species are complex, the aim of this research was to evaluate species-specific primers in PCR in order to standardize fast and reliable molecular methods for differentiation between the four Monilinia species. Isolates of M. fructigena, M. laxa, M. fructicola and M. polystroma from apple fruit and referent isolates from Italy and Japan were used for testing. Specific molecular detection of M. laxa was obtained using ITS1Mlx/ITS4Mlx and Ml-Mfg-F2/Ml-Mfc-R1 primer pairs, and M. fructicola using ITS1Mfcl/ITS4Mfcl and Mfc-F1/Mfc-R1 primer pairs. ITS1Mfgn/ITS4Mfgn and ITS1/Mfg-R2 primer pairs, described as M. fructigena species-specific, amplified M. fructigena and M. polystroma, as well. Specific detection of these two species as well as of all four tested Monilinia species was obtained using the reverse primer MO368-5 with forward primers MO368-8R, Laxa-R2 and MO368-10R in separate or in Multiplex PCR reactions., Vrste roda Monilinia su ekonomski znač ajni patogeni jabučastih i koštičavih vrsta voćaka. U Srbiji su prisutne četiri vrste ovog roda: M. fructigena, M. laxa, M. fructicola i Monilia polystroma. Detekcija i identifikacija vrsta roda Monilinia je složena, tako da je cilj ovog rada bio da se ispita i standardizuje brza i efikasna metoda molekularne detekcije korišćenjem različitih prajmera u PCR metodi i da se utvrdi njihova specifičnost i mogućnost korišćenja u identifikaciji Monilinia spp. U radu su korišćeni izolati M. fructigena, M. laxa, M. fructicola i M. polystroma poreklom iz plodova jabuke prikupljenih u Srbiji, kao i referentni izolati iz Italije i Japana. Specifična molekularna detekcija M. laxa postignuta je korišćenjem prajmera ITS1Mlx/ITS4Mlx i Ml-Mfg -F2/Ml-Mfc-R1, a vrste M. fructicola korišćenjem prajmera ITS1Mfcl/ITS4Mfcl i Mfc-F1/Mfc-R1. Prajmeri ITS1Mfgn/ITS4Mfgn i ITS1/Mfg -R2, koji su u literaturi opisani kao specifični za M. fructigena, pored izolata M. fructigena amplifikovali su i DNK izolata M. polystroma. Za razdvajanje ove dve vrste, kao i za razlikovanje sve četiri ispitivane vrste roda Monilinia, najpogodniji su uzvodni prajmer MO368-5 u kombinaciji sa nizvodnim prajmerima MO368-8R, Laxa-R2 i MO368-10R u direktnoj ili Multiplex PCR metodi.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Suitability of different primers for specific molecular detection of Monilinia spp., Pogodnost različitih prajmera za specifičnu molekularnu detekciju Monilinia spp.",
pages = "177-167",
number = "2",
volume = "62",
doi = "10.2298/JAS1702167D"
}

Duduk, N., Vasić, M. M., Vučković, N. R., Žebeljan, A.,& Vico, I.. (2017). Suitability of different primers for specific molecular detection of Monilinia spp.. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 62(2), 167-177.
https://doi.org/10.2298/JAS1702167D

Duduk N, Vasić MM, Vučković NR, Žebeljan A, Vico I. Suitability of different primers for specific molecular detection of Monilinia spp.. in Journal of Agricultural Sciences (Belgrade). 2017;62(2):167-177.
doi:10.2298/JAS1702167D .

Duduk, Nataša, Vasić, Miljan M., Vučković, Nina R., Žebeljan, Aleksandra, Vico, Ivana, "Suitability of different primers for specific molecular detection of Monilinia spp." in Journal of Agricultural Sciences (Belgrade), 62, no. 2 (2017):167-177,
https://doi.org/10.2298/JAS1702167D . .

TY  - JOUR
AU  - Duduk, Nataša
AU  - Lazarević, Marina
AU  - Žebeljan, Aleksandra
AU  - Vasić, Miljan
AU  - Vico, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4452
AB  - Onion bulbs (Allium cepa L.) can be stored for up to 12months to ensure their availability all year, but one of the limiting factors is decay caused by blue mould fungi. Postharvest development of blue mould is not only economically important, but also poses a health hazard due to mycotoxin contamination. Onion bulbs of different yellow and red cultivars with blue mould symptoms were collected from three storage facilities in Serbia. Pathogenic isolates were identified and characterized based on their molecular (partial -tubulin gene), morphological (micro- and macromorphology, and colony growth) and biochemical properties (analyses of indole metabolites via Ehrlich test). Three Penicillium species were identified: P.polonicum, P.glabrum and P.expansum. Virulence on inoculated onion bulbs varied significantly within isolates of P.glabrum, but the most virulent isolate of P.polonicum and P.glabrum did not differ, nor did they differ from an isolate of P.expansum. Variability in virulence of individual P.glabrum isolates corresponded to differences in their molecular and macromorphological characters. P.glabrum and P.expansum were identified for the first time as causal agents of onion bulb decay in Serbia. Data from this investigation provide insight into diversity of economically important and possibly toxigenic blue mould fungi which brings attention to their presence in storage facilities and therefore the necessity of the application of prevention measures.
PB  - Wiley, Hoboken
T2  - Journal of Phytopathology
T1  - Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum
EP  - 669
IS  - 10
SP  - 662
VL  - 165
DO  - 10.1111/jph.12605
ER  - 

@article{
author = "Duduk, Nataša and Lazarević, Marina and Žebeljan, Aleksandra and Vasić, Miljan and Vico, Ivana",
year = "2017",
abstract = "Onion bulbs (Allium cepa L.) can be stored for up to 12months to ensure their availability all year, but one of the limiting factors is decay caused by blue mould fungi. Postharvest development of blue mould is not only economically important, but also poses a health hazard due to mycotoxin contamination. Onion bulbs of different yellow and red cultivars with blue mould symptoms were collected from three storage facilities in Serbia. Pathogenic isolates were identified and characterized based on their molecular (partial -tubulin gene), morphological (micro- and macromorphology, and colony growth) and biochemical properties (analyses of indole metabolites via Ehrlich test). Three Penicillium species were identified: P.polonicum, P.glabrum and P.expansum. Virulence on inoculated onion bulbs varied significantly within isolates of P.glabrum, but the most virulent isolate of P.polonicum and P.glabrum did not differ, nor did they differ from an isolate of P.expansum. Variability in virulence of individual P.glabrum isolates corresponded to differences in their molecular and macromorphological characters. P.glabrum and P.expansum were identified for the first time as causal agents of onion bulb decay in Serbia. Data from this investigation provide insight into diversity of economically important and possibly toxigenic blue mould fungi which brings attention to their presence in storage facilities and therefore the necessity of the application of prevention measures.",
publisher = "Wiley, Hoboken",
journal = "Journal of Phytopathology",
title = "Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum",
pages = "669-662",
number = "10",
volume = "165",
doi = "10.1111/jph.12605"
}

Duduk, N., Lazarević, M., Žebeljan, A., Vasić, M.,& Vico, I.. (2017). Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum. in Journal of Phytopathology
Wiley, Hoboken., 165(10), 662-669.
https://doi.org/10.1111/jph.12605

Duduk N, Lazarević M, Žebeljan A, Vasić M, Vico I. Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum. in Journal of Phytopathology. 2017;165(10):662-669.
doi:10.1111/jph.12605 .

Duduk, Nataša, Lazarević, Marina, Žebeljan, Aleksandra, Vasić, Miljan, Vico, Ivana, "Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P.glabrum and P.expansum" in Journal of Phytopathology, 165, no. 10 (2017):662-669,
https://doi.org/10.1111/jph.12605 . .

TY  - JOUR
AU  - Vico, Ivana
AU  - Žebeljan, Aleksandra
AU  - Vucković, N.
AU  - Vasić, M.
AU  - Duduk, Nataša
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4496
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia
EP  - 1823
IS  - 10
SP  - 1823
VL  - 101
DO  - 10.1094/PDIS-04-17-0484-PDN
ER  - 

@article{
author = "Vico, Ivana and Žebeljan, Aleksandra and Vucković, N. and Vasić, M. and Duduk, Nataša",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia",
pages = "1823-1823",
number = "10",
volume = "101",
doi = "10.1094/PDIS-04-17-0484-PDN"
}

Vico, I., Žebeljan, A., Vucković, N., Vasić, M.,& Duduk, N.. (2017). First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(10), 1823-1823.
https://doi.org/10.1094/PDIS-04-17-0484-PDN

Vico I, Žebeljan A, Vucković N, Vasić M, Duduk N. First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia. in Plant Disease. 2017;101(10):1823-1823.
doi:10.1094/PDIS-04-17-0484-PDN .

Vico, Ivana, Žebeljan, Aleksandra, Vucković, N., Vasić, M., Duduk, Nataša, "First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia" in Plant Disease, 101, no. 10 (2017):1823-1823,
https://doi.org/10.1094/PDIS-04-17-0484-PDN . .

TY  - CONF
AU  - Vico, Ivana
AU  - Duduk, Nataša
AU  - Vasić, M.
AU  - Žebeljan, Aleksandra
AU  - Radivojević, D.
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4141
AB  - Bull's eye rot caused by Neofabraea spp. is an important postharvest disease of apple fruit worldwide. Four species of the genus are known to cause this disease among which Neofabraea alba is the main pathogen causing bull's eye rot in continental Europe. Typical symptoms of the disease were observed in a local market in Belgrade, Serbia in March 2015. Circular lesions, slightly sunken light brown to dark brown with a lighter brown to tan center and a darker outer ring were present on 'Golden Delicious' apples. Decayed tissue was firm. The aim of this study was to identify the causal agent of bull's eye rot of apple fruit based on morphological and molecular characteristics of the pathogen. Two fungal isolates were obtained using standard laboratory procedure and their pathogenicity was tested by wound inoculation of healthy 'Idared' apple fruit. Seven days post inoculation lesions up to 23 cm in diameter developed on inoculated fruit while control fruit remained healthy. The isolates were preliminary identified based on colony morphology. They formed round, slow growing colonies on potato dextrose agar (PDA) with initially white mycelium which turned pinkish-brown with time. Cylindrical to curved-fusiform macroconidia were formed. Microconidia were not observed. Species level identification was completed by sequence analysis of the partial beta-tubulin gene. MegaBLAST analysis of the obtained nucleotide sequences (622 nt) revealed 100% and 99% similarity with several Neofabraea alba sequences deposited in GenBank. Based on morphological characteristics and the partial sequence analysis of p-tubulin gene, Neofabraea alba (Guthrie) Verkley was identified as the causal agent of bull's eye rot of apple fruit in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - Bull's eye rot of apple fruit caused by Neofabraea alba
EP  - 737
SP  - 733
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.125
ER  - 

@conference{
author = "Vico, Ivana and Duduk, Nataša and Vasić, M. and Žebeljan, Aleksandra and Radivojević, D.",
year = "2016",
abstract = "Bull's eye rot caused by Neofabraea spp. is an important postharvest disease of apple fruit worldwide. Four species of the genus are known to cause this disease among which Neofabraea alba is the main pathogen causing bull's eye rot in continental Europe. Typical symptoms of the disease were observed in a local market in Belgrade, Serbia in March 2015. Circular lesions, slightly sunken light brown to dark brown with a lighter brown to tan center and a darker outer ring were present on 'Golden Delicious' apples. Decayed tissue was firm. The aim of this study was to identify the causal agent of bull's eye rot of apple fruit based on morphological and molecular characteristics of the pathogen. Two fungal isolates were obtained using standard laboratory procedure and their pathogenicity was tested by wound inoculation of healthy 'Idared' apple fruit. Seven days post inoculation lesions up to 23 cm in diameter developed on inoculated fruit while control fruit remained healthy. The isolates were preliminary identified based on colony morphology. They formed round, slow growing colonies on potato dextrose agar (PDA) with initially white mycelium which turned pinkish-brown with time. Cylindrical to curved-fusiform macroconidia were formed. Microconidia were not observed. Species level identification was completed by sequence analysis of the partial beta-tubulin gene. MegaBLAST analysis of the obtained nucleotide sequences (622 nt) revealed 100% and 99% similarity with several Neofabraea alba sequences deposited in GenBank. Based on morphological characteristics and the partial sequence analysis of p-tubulin gene, Neofabraea alba (Guthrie) Verkley was identified as the causal agent of bull's eye rot of apple fruit in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "Bull's eye rot of apple fruit caused by Neofabraea alba",
pages = "737-733",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.125"
}

Vico, I., Duduk, N., Vasić, M., Žebeljan, A.,& Radivojević, D.. (2016). Bull's eye rot of apple fruit caused by Neofabraea alba. in III Balkan Symposium on Fruit Growing
Int Soc Horticultural Science, Leuven 1., 1139, 733-737.
https://doi.org/10.17660/ActaHortic.2016.1139.125

Vico I, Duduk N, Vasić M, Žebeljan A, Radivojević D. Bull's eye rot of apple fruit caused by Neofabraea alba. in III Balkan Symposium on Fruit Growing. 2016;1139:733-737.
doi:10.17660/ActaHortic.2016.1139.125 .

Vico, Ivana, Duduk, Nataša, Vasić, M., Žebeljan, Aleksandra, Radivojević, D., "Bull's eye rot of apple fruit caused by Neofabraea alba" in III Balkan Symposium on Fruit Growing, 1139 (2016):733-737,
https://doi.org/10.17660/ActaHortic.2016.1139.125 . .