TY  - JOUR
AU  - Newberry, Eric A.
AU  - Ebrahim, Mohamed
AU  - Timilsina, Sujan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
AU  - Bull, Carolee T.
AU  - Goss, Erica M.
AU  - Huguet-Tapia, Jose C.
AU  - Paret, Mathews L.
AU  - Jones, Jeffrey B.
AU  - Potnis, Neha
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5040
AB  - Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash
VL  - 10
DO  - 10.3389/fmicb.2019.00270
ER  - 

@article{
author = "Newberry, Eric A. and Ebrahim, Mohamed and Timilsina, Sujan and Zlatković, Nevena and Obradović, Aleksa and Bull, Carolee T. and Goss, Erica M. and Huguet-Tapia, Jose C. and Paret, Mathews L. and Jones, Jeffrey B. and Potnis, Neha",
year = "2019",
abstract = "Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash",
volume = "10",
doi = "10.3389/fmicb.2019.00270"
}

Newberry, E. A., Ebrahim, M., Timilsina, S., Zlatković, N., Obradović, A., Bull, C. T., Goss, E. M., Huguet-Tapia, J. C., Paret, M. L., Jones, J. B.,& Potnis, N.. (2019). Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00270

Newberry EA, Ebrahim M, Timilsina S, Zlatković N, Obradović A, Bull CT, Goss EM, Huguet-Tapia JC, Paret ML, Jones JB, Potnis N. Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00270 .

Newberry, Eric A., Ebrahim, Mohamed, Timilsina, Sujan, Zlatković, Nevena, Obradović, Aleksa, Bull, Carolee T., Goss, Erica M., Huguet-Tapia, Jose C., Paret, Mathews L., Jones, Jeffrey B., Potnis, Neha, "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00270 . .

TY  - JOUR
AU  - Newberry, Eric A.
AU  - Ebrahim, Mohamed
AU  - Timilsina, Sujan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
AU  - Bull, Carolee T.
AU  - Goss, Erica M.
AU  - Huguet-Tapia, Jose C.
AU  - Paret, Mathews L.
AU  - Jones, Jeffrey B.
AU  - Potnis, Neha
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5045
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019)
VL  - 10
DO  - 10.3389/fmicb.2019.00963
ER  - 

@article{
author = "Newberry, Eric A. and Ebrahim, Mohamed and Timilsina, Sujan and Zlatković, Nevena and Obradović, Aleksa and Bull, Carolee T. and Goss, Erica M. and Huguet-Tapia, Jose C. and Paret, Mathews L. and Jones, Jeffrey B. and Potnis, Neha",
year = "2019",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019)",
volume = "10",
doi = "10.3389/fmicb.2019.00963"
}

Newberry, E. A., Ebrahim, M., Timilsina, S., Zlatković, N., Obradović, A., Bull, C. T., Goss, E. M., Huguet-Tapia, J. C., Paret, M. L., Jones, J. B.,& Potnis, N.. (2019). Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019). in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00963

Newberry EA, Ebrahim M, Timilsina S, Zlatković N, Obradović A, Bull CT, Goss EM, Huguet-Tapia JC, Paret ML, Jones JB, Potnis N. Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019). in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00963 .

Newberry, Eric A., Ebrahim, Mohamed, Timilsina, Sujan, Zlatković, Nevena, Obradović, Aleksa, Bull, Carolee T., Goss, Erica M., Huguet-Tapia, Jose C., Paret, Mathews L., Jones, Jeffrey B., Potnis, Neha, "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019)" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00963 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Jones, Jeffrey B.
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3716
AB  - Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).
PB  - Elsevier Gmbh, Munich
T2  - Systematic and Applied Microbiology
T1  - Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum
EP  - 378
IS  - 6
SP  - 373
VL  - 38
DO  - 10.1016/j.syapm.2015.06.001
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Jones, Jeffrey B. and Obradović, Aleksa",
year = "2015",
abstract = "Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).",
publisher = "Elsevier Gmbh, Munich",
journal = "Systematic and Applied Microbiology",
title = "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum",
pages = "378-373",
number = "6",
volume = "38",
doi = "10.1016/j.syapm.2015.06.001"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Jones, J. B.,& Obradović, A.. (2015). Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology
Elsevier Gmbh, Munich., 38(6), 373-378.
https://doi.org/10.1016/j.syapm.2015.06.001

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Jones JB, Obradović A. Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology. 2015;38(6):373-378.
doi:10.1016/j.syapm.2015.06.001 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Jones, Jeffrey B., Obradović, Aleksa, "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum" in Systematic and Applied Microbiology, 38, no. 6 (2015):373-378,
https://doi.org/10.1016/j.syapm.2015.06.001 . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3017
AB  - Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that the E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns
EP  - 557
IS  - 3
SP  - 545
VL  - 133
DO  - 10.1007/s10658-011-9926-8
ER  - 

@article{
author = "Ivanović, Milan and Obradović, Aleksa and Gašić, Katarina and Minsavage, Gerald V. and Dickstein, Ellen R. and Jones, Jeffrey B.",
year = "2012",
abstract = "Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that the E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns",
pages = "557-545",
number = "3",
volume = "133",
doi = "10.1007/s10658-011-9926-8"
}

Ivanović, M., Obradović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns. in European Journal of Plant Pathology
Springer, Dordrecht., 133(3), 545-557.
https://doi.org/10.1007/s10658-011-9926-8

Ivanović M, Obradović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns. in European Journal of Plant Pathology. 2012;133(3):545-557.
doi:10.1007/s10658-011-9926-8 .

Ivanović, Milan, Obradović, Aleksa, Gašić, Katarina, Minsavage, Gerald V., Dickstein, Ellen R., Jones, Jeffrey B., "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns" in European Journal of Plant Pathology, 133, no. 3 (2012):545-557,
https://doi.org/10.1007/s10658-011-9926-8 . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Obadović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3016
AB  - Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns
EP  - 727
IS  - 3
SP  - 715
VL  - 133
DO  - 10.1007/s10658-012-9950-3
ER  - 

@article{
author = "Ivanović, Milan and Obadović, Aleksa and Gašić, Katarina and Minsavage, Gerald V. and Dickstein, Ellen R. and Jones, Jeffrey B.",
year = "2012",
abstract = "Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns",
pages = "727-715",
number = "3",
volume = "133",
doi = "10.1007/s10658-012-9950-3"
}

Ivanović, M., Obadović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns. in European Journal of Plant Pathology
Springer, Dordrecht., 133(3), 715-727.
https://doi.org/10.1007/s10658-012-9950-3

Ivanović M, Obadović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns. in European Journal of Plant Pathology. 2012;133(3):715-727.
doi:10.1007/s10658-012-9950-3 .

Ivanović, Milan, Obadović, Aleksa, Gašić, Katarina, Minsavage, Gerald V., Dickstein, Ellen R., Jones, Jeffrey B., "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns" in European Journal of Plant Pathology, 133, no. 3 (2012):715-727,
https://doi.org/10.1007/s10658-012-9950-3 . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3008
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)
EP  - 443
IS  - 2
SP  - 443
VL  - 134
DO  - 10.1007/s10658-012-0037-y
ER  - 

@article{
author = "Ivanović, Milan and Obradović, Aleksa and Gašić, Katarina and Minsavage, Gerald V. and Dickstein, Ellen R. and Jones, Jeffrey B.",
year = "2012",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)",
pages = "443-443",
number = "2",
volume = "134",
doi = "10.1007/s10658-012-0037-y"
}

Ivanović, M., Obradović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012). in European Journal of Plant Pathology
Springer, Dordrecht., 134(2), 443-443.
https://doi.org/10.1007/s10658-012-0037-y

Ivanović M, Obradović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012). in European Journal of Plant Pathology. 2012;134(2):443-443.
doi:10.1007/s10658-012-0037-y .

Ivanović, Milan, Obradović, Aleksa, Gašić, Katarina, Minsavage, Gerald V., Dickstein, Ellen R., Jones, Jeffrey B., "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)" in European Journal of Plant Pathology, 134, no. 2 (2012):443-443,
https://doi.org/10.1007/s10658-012-0037-y . .

TY  - CONF
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Obradović, Aleksa
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
AU  - Gavrilović, Veljko
AU  - Balaž, Jelica
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2441
AB  - A collection of 41 Erwinia amylovora strains isolated from 8 plant species at 13 locations in Serbia and one location in Montenegro was tested and their relationship was determined based on carbohydrate metabolism and fatty acid composition. Metabolism of carbon compounds was tested using the Biolog GN MicroPlate system. After 24 h of incubation, the resulting metabolic fingerprints were recorded and compared with the Biolog GN database. Fatty acid composition was analyzed by Microbial Identification System and compared with the data from the MIDI database. Our results showed that all strains tested were identified as E. amylovora, with similarity index (SI) between 0.62 and 0.99, using BIOLOG. Based on fatty acid composition, 39 strains were identified as E. amylovora, with SI between 0.61 and 0.92. More than 14 fatty acids were detected, eight of which were present in all strains with more than 1% of the total named peak area. Based on quantitative and qualitative content of fatty acid composition, the isolates were clustered into three groups: alpha, beta and gamma (Euclidian distance  lt = 6). Cluster analysis revealed that groups beta and gamma consists of only strains isolated from north Serbia, whereas all strains isolated from central or south Serbia belong to group alpha.
C3  - Acta Horticulturae
T1  - Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG
EP  - 72
SP  - 65
VL  - 896
DO  - 10.17660/actahortic.2011.896.6
ER  - 

@conference{
author = "Ivanović, Milan and Gašić, K. and Obradović, Aleksa and Dickstein, Ellen R. and Jones, Jeffrey B. and Gavrilović, Veljko and Balaž, Jelica",
year = "2011",
abstract = "A collection of 41 Erwinia amylovora strains isolated from 8 plant species at 13 locations in Serbia and one location in Montenegro was tested and their relationship was determined based on carbohydrate metabolism and fatty acid composition. Metabolism of carbon compounds was tested using the Biolog GN MicroPlate system. After 24 h of incubation, the resulting metabolic fingerprints were recorded and compared with the Biolog GN database. Fatty acid composition was analyzed by Microbial Identification System and compared with the data from the MIDI database. Our results showed that all strains tested were identified as E. amylovora, with similarity index (SI) between 0.62 and 0.99, using BIOLOG. Based on fatty acid composition, 39 strains were identified as E. amylovora, with SI between 0.61 and 0.92. More than 14 fatty acids were detected, eight of which were present in all strains with more than 1% of the total named peak area. Based on quantitative and qualitative content of fatty acid composition, the isolates were clustered into three groups: alpha, beta and gamma (Euclidian distance  lt = 6). Cluster analysis revealed that groups beta and gamma consists of only strains isolated from north Serbia, whereas all strains isolated from central or south Serbia belong to group alpha.",
journal = "Acta Horticulturae",
title = "Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG",
pages = "72-65",
volume = "896",
doi = "10.17660/actahortic.2011.896.6"
}

Ivanović, M., Gašić, K., Obradović, A., Dickstein, E. R., Jones, J. B., Gavrilović, V.,& Balaž, J.. (2011). Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG. in Acta Horticulturae, 896, 65-72.
https://doi.org/10.17660/actahortic.2011.896.6

Ivanović M, Gašić K, Obradović A, Dickstein ER, Jones JB, Gavrilović V, Balaž J. Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG. in Acta Horticulturae. 2011;896:65-72.
doi:10.17660/actahortic.2011.896.6 .

Ivanović, Milan, Gašić, K., Obradović, Aleksa, Dickstein, Ellen R., Jones, Jeffrey B., Gavrilović, Veljko, Balaž, Jelica, "Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG" in Acta Horticulturae, 896 (2011):65-72,
https://doi.org/10.17660/actahortic.2011.896.6 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Balogh, B.
AU  - Momol, Timur M.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1846
AB  - Tomato bacterial spot, caused by Xanthomonas spp. complex, is a constant threat to tomato (Solanum lycopersicum) commercial production, but is especially severe in Florida and the southeastern US when weather conditions become conducive for disease development. In an effort to develop more sustainable strategies for reducing severity of the disease, we investigated various combinations of PGPR strains, bacterial antagonists, bacteriophages and SAR inducers (harpin, acibenzolar-S-methyl) in greenhouse and field trials. The idea was to search for alternative treatments that could be integrated with conventional practices, in order to improve disease control and yield responses. After screening single treatments and their combinations for efficacy in a series of greenhouse experiments, acibenzolar-S-methyl (ASM) and phage treatment provided the most promising results. In field trials, carried out in three consecutive seasons, ASM significantly reduced disease severity compared to the untreated control. However, the combination of ASM and formulated host-specific phages provided an additional reduction in disease pressure and resulted in more efficient foliar disease control than ASM, phage, or copper-macozeb alone. Integrated application of phages, ASM and other practices is currently widely used in greenhouses and production fields in Florida as a part of a standard integrated management strategy for tomato bacterial spot control. http://www.actahort.org/books/808/index.htm
T2  - Acta Horticulturae
T1  - Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice
EP  - 346
SP  - 343
VL  - 808
DO  - 10.17660/ActaHortic.2009.808.55
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Balogh, B. and Momol, Timur M.",
year = "2009",
abstract = "Tomato bacterial spot, caused by Xanthomonas spp. complex, is a constant threat to tomato (Solanum lycopersicum) commercial production, but is especially severe in Florida and the southeastern US when weather conditions become conducive for disease development. In an effort to develop more sustainable strategies for reducing severity of the disease, we investigated various combinations of PGPR strains, bacterial antagonists, bacteriophages and SAR inducers (harpin, acibenzolar-S-methyl) in greenhouse and field trials. The idea was to search for alternative treatments that could be integrated with conventional practices, in order to improve disease control and yield responses. After screening single treatments and their combinations for efficacy in a series of greenhouse experiments, acibenzolar-S-methyl (ASM) and phage treatment provided the most promising results. In field trials, carried out in three consecutive seasons, ASM significantly reduced disease severity compared to the untreated control. However, the combination of ASM and formulated host-specific phages provided an additional reduction in disease pressure and resulted in more efficient foliar disease control than ASM, phage, or copper-macozeb alone. Integrated application of phages, ASM and other practices is currently widely used in greenhouses and production fields in Florida as a part of a standard integrated management strategy for tomato bacterial spot control. http://www.actahort.org/books/808/index.htm",
journal = "Acta Horticulturae",
title = "Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice",
pages = "346-343",
volume = "808",
doi = "10.17660/ActaHortic.2009.808.55"
}

Obradović, A., Jones, J. B., Balogh, B.,& Momol, T. M.. (2009). Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice. in Acta Horticulturae, 808, 343-346.
https://doi.org/10.17660/ActaHortic.2009.808.55

Obradović A, Jones JB, Balogh B, Momol TM. Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice. in Acta Horticulturae. 2009;808:343-346.
doi:10.17660/ActaHortic.2009.808.55 .

Obradović, Aleksa, Jones, Jeffrey B., Balogh, B., Momol, Timur M., "Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice" in Acta Horticulturae, 808 (2009):343-346,
https://doi.org/10.17660/ActaHortic.2009.808.55 . .

TY  - CHAP
AU  - Balogh, B.
AU  - Momol, Timur M.
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1843
T2  - Disease Control in Crops: Biological and Environmentally-Friendly Approaches
T1  - Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria
EP  - 256
SP  - 246
DO  - 10.1002/9781444312157.ch13
ER  - 

@inbook{
author = "Balogh, B. and Momol, Timur M. and Obradović, Aleksa and Jones, Jeffrey B.",
year = "2009",
journal = "Disease Control in Crops: Biological and Environmentally-Friendly Approaches",
booktitle = "Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria",
pages = "256-246",
doi = "10.1002/9781444312157.ch13"
}

Balogh, B., Momol, T. M., Obradović, A.,& Jones, J. B.. (2009). Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria. in Disease Control in Crops: Biological and Environmentally-Friendly Approaches, 246-256.
https://doi.org/10.1002/9781444312157.ch13

Balogh B, Momol TM, Obradović A, Jones JB. Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria. in Disease Control in Crops: Biological and Environmentally-Friendly Approaches. 2009;:246-256.
doi:10.1002/9781444312157.ch13 .

Balogh, B., Momol, Timur M., Obradović, Aleksa, Jones, Jeffrey B., "Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria" in Disease Control in Crops: Biological and Environmentally-Friendly Approaches (2009):246-256,
https://doi.org/10.1002/9781444312157.ch13 . .

TY  - JOUR
AU  - Jones, Jeffrey B.
AU  - Jackson, LE
AU  - Balogh, B.
AU  - Obradović, Aleksa
AU  - Iriarte, FB
AU  - Momol, Timur M.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1598
AB  - The use of phages for disease control is a fast expanding area of plant protection with great potential to replace the chemical control measures now prevalent. Phages can be used effectively as part of integrated disease management strategies. The relative ease of preparing phage treatments and low cost of production of these agents make them good candidates for widespread use in developing countries as well. However, the efficacy of phages, as is true of many biological control agents, depends greatly on prevailing environmental factors as well as on susceptibility of the target organism. Great care is necessary during development, production and application of phage treatments. In addition, constant monitoring for the emergence of resistant bacterial strains is essential. Phage-based disease control management is a dynamic process with a need for continuous adjustment of the phage preparation in order to effectively fight potentially adapting pathogenic bacteria.
PB  - Annual Reviews, Palo Alto
T2  - Annual Review of Phytopathology
T1  - Bacteriophages for plant disease control
EP  - 262
SP  - 245
VL  - 45
DO  - 10.1146/annurev.phyto.45.062806.094411
ER  - 

@article{
author = "Jones, Jeffrey B. and Jackson, LE and Balogh, B. and Obradović, Aleksa and Iriarte, FB and Momol, Timur M.",
year = "2007",
abstract = "The use of phages for disease control is a fast expanding area of plant protection with great potential to replace the chemical control measures now prevalent. Phages can be used effectively as part of integrated disease management strategies. The relative ease of preparing phage treatments and low cost of production of these agents make them good candidates for widespread use in developing countries as well. However, the efficacy of phages, as is true of many biological control agents, depends greatly on prevailing environmental factors as well as on susceptibility of the target organism. Great care is necessary during development, production and application of phage treatments. In addition, constant monitoring for the emergence of resistant bacterial strains is essential. Phage-based disease control management is a dynamic process with a need for continuous adjustment of the phage preparation in order to effectively fight potentially adapting pathogenic bacteria.",
publisher = "Annual Reviews, Palo Alto",
journal = "Annual Review of Phytopathology",
title = "Bacteriophages for plant disease control",
pages = "262-245",
volume = "45",
doi = "10.1146/annurev.phyto.45.062806.094411"
}

Jones, J. B., Jackson, L., Balogh, B., Obradović, A., Iriarte, F.,& Momol, T. M.. (2007). Bacteriophages for plant disease control. in Annual Review of Phytopathology
Annual Reviews, Palo Alto., 45, 245-262.
https://doi.org/10.1146/annurev.phyto.45.062806.094411

Jones JB, Jackson L, Balogh B, Obradović A, Iriarte F, Momol TM. Bacteriophages for plant disease control. in Annual Review of Phytopathology. 2007;45:245-262.
doi:10.1146/annurev.phyto.45.062806.094411 .

Jones, Jeffrey B., Jackson, LE, Balogh, B., Obradović, Aleksa, Iriarte, FB, Momol, Timur M., "Bacteriophages for plant disease control" in Annual Review of Phytopathology, 45 (2007):245-262,
https://doi.org/10.1146/annurev.phyto.45.062806.094411 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Momol, Timur M.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1499
AB  - A leaf spot and blighting were observed on leaves of tomato transplants from a producer in Florida in 2001 and 2002. A nonfluorescent bacterium was isolated consistently from affected tissue. The typical bacterium was a gram negative, strictly aerobic, slightly curved rod with one or two flagella. Sequence analysis of the 16S rRNA indicated that two representative strains, F1 and SE1, had greater than 99% nucleotide sequence identity with Herbaspirillum huttiense and H. rubrisubalbicans. The cellular fatty acid composition of the total of 16 tomato strains was very similar to H. huttiense and H. rubrisubalbicans. Based on carbon utilization, six of nine strains tested with the Biolog system were identified as Herbaspirillum spp. The tomato strains were oxidase positive and grew at 40 degrees C, but were negative for levan production, pectate hydrolysis, and arginine dihydrolase activity. Based upon this polyphasic analysis, we concluded that the strains were most closely related to H. huttiense, although placement in this species would require further analyses. However, the tomato strains and H. rubrisubalbicans, but not H. huttiense, caused confluent necrosis when infiltrated at high concentrations into tomato leaves and were able to produce leaf spot symptoms on inoculated tomato seedlings in the greenhouse. Using pulsed-field gel electrophoresis, we determined that there was considerable variability between the strains collected in 2001 and 2002.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.
EP  - 890
IS  - 7
SP  - 886
VL  - 91
DO  - 10.1094/PDIS-91-7-0886
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Minsavage, Gerald V. and Dickstein, Ellen R. and Momol, Timur M.",
year = "2007",
abstract = "A leaf spot and blighting were observed on leaves of tomato transplants from a producer in Florida in 2001 and 2002. A nonfluorescent bacterium was isolated consistently from affected tissue. The typical bacterium was a gram negative, strictly aerobic, slightly curved rod with one or two flagella. Sequence analysis of the 16S rRNA indicated that two representative strains, F1 and SE1, had greater than 99% nucleotide sequence identity with Herbaspirillum huttiense and H. rubrisubalbicans. The cellular fatty acid composition of the total of 16 tomato strains was very similar to H. huttiense and H. rubrisubalbicans. Based on carbon utilization, six of nine strains tested with the Biolog system were identified as Herbaspirillum spp. The tomato strains were oxidase positive and grew at 40 degrees C, but were negative for levan production, pectate hydrolysis, and arginine dihydrolase activity. Based upon this polyphasic analysis, we concluded that the strains were most closely related to H. huttiense, although placement in this species would require further analyses. However, the tomato strains and H. rubrisubalbicans, but not H. huttiense, caused confluent necrosis when infiltrated at high concentrations into tomato leaves and were able to produce leaf spot symptoms on inoculated tomato seedlings in the greenhouse. Using pulsed-field gel electrophoresis, we determined that there was considerable variability between the strains collected in 2001 and 2002.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.",
pages = "890-886",
number = "7",
volume = "91",
doi = "10.1094/PDIS-91-7-0886"
}

Obradović, A., Jones, J. B., Minsavage, G. V., Dickstein, E. R.,& Momol, T. M.. (2007). A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.. in Plant Disease
Amer Phytopathological Soc, St Paul., 91(7), 886-890.
https://doi.org/10.1094/PDIS-91-7-0886

Obradović A, Jones JB, Minsavage GV, Dickstein ER, Momol TM. A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.. in Plant Disease. 2007;91(7):886-890.
doi:10.1094/PDIS-91-7-0886 .

Obradović, Aleksa, Jones, Jeffrey B., Minsavage, Gerald V., Dickstein, Ellen R., Momol, Timur M., "A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp." in Plant Disease, 91, no. 7 (2007):886-890,
https://doi.org/10.1094/PDIS-91-7-0886 . .

TY  - CONF
AU  - Balogh, B.
AU  - Iriarte, FB
AU  - Obradović, Aleksa
AU  - Momol, Timur M.
AU  - Jones, Jeffrey B.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1490
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Phages don't have it easy.
EP  - S141
IS  - 7
SP  - S141
VL  - 97
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1490
ER  - 

@conference{
author = "Balogh, B. and Iriarte, FB and Obradović, Aleksa and Momol, Timur M. and Jones, Jeffrey B.",
year = "2007",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Phages don't have it easy.",
pages = "S141-S141",
number = "7",
volume = "97",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1490"
}

Balogh, B., Iriarte, F., Obradović, A., Momol, T. M.,& Jones, J. B.. (2007). Phages don't have it easy.. in Phytopathology
Amer Phytopathological Soc, St Paul., 97(7), S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_1490

Balogh B, Iriarte F, Obradović A, Momol TM, Jones JB. Phages don't have it easy.. in Phytopathology. 2007;97(7):S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_1490 .

Balogh, B., Iriarte, FB, Obradović, Aleksa, Momol, Timur M., Jones, Jeffrey B., "Phages don't have it easy." in Phytopathology, 97, no. 7 (2007):S141-S141,
https://hdl.handle.net/21.15107/rcub_agrospace_1490 .

TY  - CONF
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Obradović, Aleksa
AU  - Balogh, B
AU  - Olson, SM
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1110
AB  - Bacterial spot of tomato, incited by Xanthomonas campestris pv. vesicatoria, is a perennial problem in commercial fields in Florida. Various strategies have been used to control this disease including applying copper bactericides, antibiotics and various other chemicals. Although copper compounds and streptomycin were initially effective in disease control, the presence of strains resistant to these compounds has reduced the efficacy of these compounds. Systemic acquired resistance (SAR) compounds have become a popular alternative to conventional bactericides. One that has shown promise for bacterial spot control is acibenzolar-S-methyl (ASM). Other SAR compounds tested have had variable results. A second strategy which has excellent promise is the application of bacteriophages specific to the bacterial spot pathogen. Following the development of an application strategy, which included a modification in formulation and the timing of bacteriophage applications, bacteriophages were improved in efficacy. We have conducted studies using various combinations of bacteriophages and SAR compounds. In three consecutive seasons, ASM applied in combination with bacteriophage significantly reduced bacterial spot compared to the other treatments. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared to the results of the corresponding non-bacteriophage group, the former provided significantly better disease control and yield of total marketable fruits. Although ASM applications controlled bacterial spot,yield was not significantly improved compared to plots receiving no ASM applications. Bacteriophages offer an excellent alternative to conventional disease control strategies and combining it with ASM shows promise.
PB  - International Society Horticultural Science, Leuven 1
C3  - Proceedings of the 1st International Symposium on Tomato Diseases
T1  - Bacterial spot management on tomatoes
EP  - 124
IS  - 695
SP  - 119
DO  - 10.17660/ActaHortic.2005.695.13
ER  - 

@conference{
author = "Jones, Jeffrey B. and Momol, Timur M. and Obradović, Aleksa and Balogh, B and Olson, SM",
year = "2005",
abstract = "Bacterial spot of tomato, incited by Xanthomonas campestris pv. vesicatoria, is a perennial problem in commercial fields in Florida. Various strategies have been used to control this disease including applying copper bactericides, antibiotics and various other chemicals. Although copper compounds and streptomycin were initially effective in disease control, the presence of strains resistant to these compounds has reduced the efficacy of these compounds. Systemic acquired resistance (SAR) compounds have become a popular alternative to conventional bactericides. One that has shown promise for bacterial spot control is acibenzolar-S-methyl (ASM). Other SAR compounds tested have had variable results. A second strategy which has excellent promise is the application of bacteriophages specific to the bacterial spot pathogen. Following the development of an application strategy, which included a modification in formulation and the timing of bacteriophage applications, bacteriophages were improved in efficacy. We have conducted studies using various combinations of bacteriophages and SAR compounds. In three consecutive seasons, ASM applied in combination with bacteriophage significantly reduced bacterial spot compared to the other treatments. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared to the results of the corresponding non-bacteriophage group, the former provided significantly better disease control and yield of total marketable fruits. Although ASM applications controlled bacterial spot,yield was not significantly improved compared to plots receiving no ASM applications. Bacteriophages offer an excellent alternative to conventional disease control strategies and combining it with ASM shows promise.",
publisher = "International Society Horticultural Science, Leuven 1",
journal = "Proceedings of the 1st International Symposium on Tomato Diseases",
title = "Bacterial spot management on tomatoes",
pages = "124-119",
number = "695",
doi = "10.17660/ActaHortic.2005.695.13"
}

Jones, J. B., Momol, T. M., Obradović, A., Balogh, B.,& Olson, S.. (2005). Bacterial spot management on tomatoes. in Proceedings of the 1st International Symposium on Tomato Diseases
International Society Horticultural Science, Leuven 1.(695), 119-124.
https://doi.org/10.17660/ActaHortic.2005.695.13

Jones JB, Momol TM, Obradović A, Balogh B, Olson S. Bacterial spot management on tomatoes. in Proceedings of the 1st International Symposium on Tomato Diseases. 2005;(695):119-124.
doi:10.17660/ActaHortic.2005.695.13 .

Jones, Jeffrey B., Momol, Timur M., Obradović, Aleksa, Balogh, B, Olson, SM, "Bacterial spot management on tomatoes" in Proceedings of the 1st International Symposium on Tomato Diseases, no. 695 (2005):119-124,
https://doi.org/10.17660/ActaHortic.2005.695.13 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Olson, SM
AU  - Jackson, LE
AU  - Balogh, B
AU  - Guven, K
AU  - Iriarte, FB
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1017
AB  - Two strains of plant growth-promoting rhizobacteria, two systemic acquired resistance inducers (harpin and acibenzolar-S-methyl), host-specific unformulated bacteriophages, and two antagonistic bacteria were evaluated for control of tomato bacterial spot incited by Xanthomonas campestris pv. vesicatoria in greenhouse experiments. Untreated plants and plants treated with copper hydroxide were used as controls. The plant growth-promoting rhizobacteria or a tap water control were applied as a drench to the potting mix containing the seedlings, while the other treatments were applied to the foliage using a handheld sprayer. The plant growth-promoting rhizobacteria strains, when applied alone or in combination with other treatments, had no significant effect on bacterial spot intensity. Messenger and the antagonistic bacterial strains, when applied alone, had negligible effects on disease intensity. Unformulated phage or copper bactericide applications were inconsistent in performance under greenhouse conditions against bacterial spot. Although acibenzolar-S-methyl completely prevented occurrence of typical symptoms of the disease, necrotic spots typical of a hypersensitive reaction (HR) were observed on plants treated with acibenzolar-S-methyl alone. Electrolyte leakage and population dynamics experiments confirmed that acibenzolar-S-methyl-treated plants responded to inoculation by eliciting an HR. Application of bacteriophages in combination with acibenzolar-S-methyl suppressed a visible HR and provided excellent disease control. Although we were unable to quantify populations of the bacterium on the leaf surface, indirectly we determined that bacteriophages specific to the target bacterium reduced populations of a tomato race 3 strain of the pathogen on the leaf surface of acibenzolar-S-methyl-treated plants to levels that did not induce a visible HR. Integrated use of acibenzolar-S-m ethyl and phages may complement each other as an alternative management strategy against bacterial spot on tomato.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato
EP  - 716
IS  - 7
SP  - 712
VL  - 89
DO  - 10.1094/PD-89-0712
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Momol, Timur M. and Olson, SM and Jackson, LE and Balogh, B and Guven, K and Iriarte, FB",
year = "2005",
abstract = "Two strains of plant growth-promoting rhizobacteria, two systemic acquired resistance inducers (harpin and acibenzolar-S-methyl), host-specific unformulated bacteriophages, and two antagonistic bacteria were evaluated for control of tomato bacterial spot incited by Xanthomonas campestris pv. vesicatoria in greenhouse experiments. Untreated plants and plants treated with copper hydroxide were used as controls. The plant growth-promoting rhizobacteria or a tap water control were applied as a drench to the potting mix containing the seedlings, while the other treatments were applied to the foliage using a handheld sprayer. The plant growth-promoting rhizobacteria strains, when applied alone or in combination with other treatments, had no significant effect on bacterial spot intensity. Messenger and the antagonistic bacterial strains, when applied alone, had negligible effects on disease intensity. Unformulated phage or copper bactericide applications were inconsistent in performance under greenhouse conditions against bacterial spot. Although acibenzolar-S-methyl completely prevented occurrence of typical symptoms of the disease, necrotic spots typical of a hypersensitive reaction (HR) were observed on plants treated with acibenzolar-S-methyl alone. Electrolyte leakage and population dynamics experiments confirmed that acibenzolar-S-methyl-treated plants responded to inoculation by eliciting an HR. Application of bacteriophages in combination with acibenzolar-S-methyl suppressed a visible HR and provided excellent disease control. Although we were unable to quantify populations of the bacterium on the leaf surface, indirectly we determined that bacteriophages specific to the target bacterium reduced populations of a tomato race 3 strain of the pathogen on the leaf surface of acibenzolar-S-methyl-treated plants to levels that did not induce a visible HR. Integrated use of acibenzolar-S-m ethyl and phages may complement each other as an alternative management strategy against bacterial spot on tomato.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato",
pages = "716-712",
number = "7",
volume = "89",
doi = "10.1094/PD-89-0712"
}

Obradović, A., Jones, J. B., Momol, T. M., Olson, S., Jackson, L., Balogh, B., Guven, K.,& Iriarte, F.. (2005). Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. in Plant Disease
Amer Phytopathological Soc, St Paul., 89(7), 712-716.
https://doi.org/10.1094/PD-89-0712

Obradović A, Jones JB, Momol TM, Olson S, Jackson L, Balogh B, Guven K, Iriarte F. Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. in Plant Disease. 2005;89(7):712-716.
doi:10.1094/PD-89-0712 .

Obradović, Aleksa, Jones, Jeffrey B., Momol, Timur M., Olson, SM, Jackson, LE, Balogh, B, Guven, K, Iriarte, FB, "Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato" in Plant Disease, 89, no. 7 (2005):712-716,
https://doi.org/10.1094/PD-89-0712 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Balogh, B
AU  - Olson, SM
PY  - 2004
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/851
AB  - Various combinations of the harpin protein, acibenzolar-S-methyl, and bacteriophages were compared for controlling tomato bacterial spot in field experiments. Harpin protein and acibenzolar-S-methyl were applied every 14 days beginning twice before transplanting and then an additional four applications throughout the season. Formulated bacteriophages were applied prior to inoculation followed by twice a week at dusk. A standard bactericide treatment, consisting of copper hydroxide plus mancozeb, was applied once prior to inoculation and then every 7 days, while untreated plants served as an untreated control. Experiments were conducted in north and central Florida fields during fall 2001, spring 2002, and fall 2002. In three consecutive seasons, acibenzolar-S-methyl applied in combination with bacteriophage or bacteriophage and harpin significantly reduced bacterial spot compared with the other treatments. However, it did not significantly affect the total yield compared with the standard or untreated control. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all three experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared with the results of the corresponding nonbacteriophage group, the former provided significantly better disease control and yield of total marketable fruit.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers
EP  - 740
IS  - 7
SP  - 736
VL  - 88
DO  - 10.1094/PDIS.2004.88.7.736
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Momol, Timur M. and Balogh, B and Olson, SM",
year = "2004",
abstract = "Various combinations of the harpin protein, acibenzolar-S-methyl, and bacteriophages were compared for controlling tomato bacterial spot in field experiments. Harpin protein and acibenzolar-S-methyl were applied every 14 days beginning twice before transplanting and then an additional four applications throughout the season. Formulated bacteriophages were applied prior to inoculation followed by twice a week at dusk. A standard bactericide treatment, consisting of copper hydroxide plus mancozeb, was applied once prior to inoculation and then every 7 days, while untreated plants served as an untreated control. Experiments were conducted in north and central Florida fields during fall 2001, spring 2002, and fall 2002. In three consecutive seasons, acibenzolar-S-methyl applied in combination with bacteriophage or bacteriophage and harpin significantly reduced bacterial spot compared with the other treatments. However, it did not significantly affect the total yield compared with the standard or untreated control. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all three experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared with the results of the corresponding nonbacteriophage group, the former provided significantly better disease control and yield of total marketable fruit.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers",
pages = "740-736",
number = "7",
volume = "88",
doi = "10.1094/PDIS.2004.88.7.736"
}

Obradović, A., Jones, J. B., Momol, T. M., Balogh, B.,& Olson, S.. (2004). Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers. in Plant Disease
Amer Phytopathological Soc, St Paul., 88(7), 736-740.
https://doi.org/10.1094/PDIS.2004.88.7.736

Obradović A, Jones JB, Momol TM, Balogh B, Olson S. Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers. in Plant Disease. 2004;88(7):736-740.
doi:10.1094/PDIS.2004.88.7.736 .

Obradović, Aleksa, Jones, Jeffrey B., Momol, Timur M., Balogh, B, Olson, SM, "Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers" in Plant Disease, 88, no. 7 (2004):736-740,
https://doi.org/10.1094/PDIS.2004.88.7.736 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Mavridis, A
AU  - Rudolph, K
AU  - Janse, JD
AU  - Arsenijević, M.
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Wang, JF
PY  - 2004
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/820
AB  - During the last two decades bacterial strains associated with necrotic leaf spots of pepper and tomato fruit spots were collected in Serbia. Twenty-eight strains isolated from pepper and six from tomato were characterized. A study of their physiological and pathological characteristics, and fatty acid composition analysis revealed that all of the strains belong to Xanthomonas campestris pv. vesicatoria. Being non-amylolytic and non-pectolytic, pathogenic on pepper but not on tomato, containing lower amounts of fatty acid 15 : 0 ante-iso, the pepper strains were designated as members of the A group of X. campestris pv. vesicatoria. However, the tomato strains hydrolyzed starch and pectate, caused compatible reactions on tomato but not on pepper, had higher percent of 15 : 0 ante iso fatty acid, and were classified into B phenotypic group and identified as X. vesicatoria. PCR primers were developed which amplified conserved DNA regions related to the hrp genes of different strains of X. campestris pv. vesicatoria associated with pepper and tomato. Restriction analysis of the PCR product resulted in different patterns and enabled grouping of the strains into four groups. When xanthomonads isolated from pepper and tomato in Serbia were analyzed, they clustered into two groups corresponding to the grouping based on their physiological and pathological characteristics. According to the reaction of pepper and tomato differential varieties, the strains from pepper belong to races P7 and P8 and tomato strains belong to the race T2. All strains were sensitive to copper and streptomycin. Advantages and disadvantages of various bacterial spot management practices are discussed.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia
EP  - 292
IS  - 3
SP  - 285
VL  - 110
DO  - 10.1023/B:EJPP.0000019797.27952.1d
ER  - 

@article{
author = "Obradović, Aleksa and Mavridis, A and Rudolph, K and Janse, JD and Arsenijević, M. and Jones, Jeffrey B. and Minsavage, Gerald V. and Wang, JF",
year = "2004",
abstract = "During the last two decades bacterial strains associated with necrotic leaf spots of pepper and tomato fruit spots were collected in Serbia. Twenty-eight strains isolated from pepper and six from tomato were characterized. A study of their physiological and pathological characteristics, and fatty acid composition analysis revealed that all of the strains belong to Xanthomonas campestris pv. vesicatoria. Being non-amylolytic and non-pectolytic, pathogenic on pepper but not on tomato, containing lower amounts of fatty acid 15 : 0 ante-iso, the pepper strains were designated as members of the A group of X. campestris pv. vesicatoria. However, the tomato strains hydrolyzed starch and pectate, caused compatible reactions on tomato but not on pepper, had higher percent of 15 : 0 ante iso fatty acid, and were classified into B phenotypic group and identified as X. vesicatoria. PCR primers were developed which amplified conserved DNA regions related to the hrp genes of different strains of X. campestris pv. vesicatoria associated with pepper and tomato. Restriction analysis of the PCR product resulted in different patterns and enabled grouping of the strains into four groups. When xanthomonads isolated from pepper and tomato in Serbia were analyzed, they clustered into two groups corresponding to the grouping based on their physiological and pathological characteristics. According to the reaction of pepper and tomato differential varieties, the strains from pepper belong to races P7 and P8 and tomato strains belong to the race T2. All strains were sensitive to copper and streptomycin. Advantages and disadvantages of various bacterial spot management practices are discussed.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia",
pages = "292-285",
number = "3",
volume = "110",
doi = "10.1023/B:EJPP.0000019797.27952.1d"
}

Obradović, A., Mavridis, A., Rudolph, K., Janse, J., Arsenijević, M., Jones, J. B., Minsavage, G. V.,& Wang, J.. (2004). Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 110(3), 285-292.
https://doi.org/10.1023/B:EJPP.0000019797.27952.1d

Obradović A, Mavridis A, Rudolph K, Janse J, Arsenijević M, Jones JB, Minsavage GV, Wang J. Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. in European Journal of Plant Pathology. 2004;110(3):285-292.
doi:10.1023/B:EJPP.0000019797.27952.1d .

Obradović, Aleksa, Mavridis, A, Rudolph, K, Janse, JD, Arsenijević, M., Jones, Jeffrey B., Minsavage, Gerald V., Wang, JF, "Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia" in European Journal of Plant Pathology, 110, no. 3 (2004):285-292,
https://doi.org/10.1023/B:EJPP.0000019797.27952.1d . .

TY  - JOUR
AU  - Balogh, B
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Olson, SM
AU  - Obradović, Aleksa
AU  - King, P
AU  - Jackson, LE
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/641
AB  - Bacteriophages are currently used as an alternative method for controlling bacterial spot disease on tomato incited by Xanthomonas campestris pv. vesicatoria. However, the efficacy of phage is greatly reduced due to its short residual activity on plant foliage. Three formulations that significantly increased phage longevity on the plant surface were tested in field and greenhouse trials: (i) PCF, 0.5% pregelatinized corn flour (PCF) + 0.5% sucrose; (ii) Casecrete, 0.5% Casecrete NH-400 + 0.5% sucrose + 0.25% PCF; and (iii) skim milk, 0.75% powdered skim milk + 0.5% sucrose. In greenhouse experiments, the nonformulated, PCF-, Casecrete-, and skim milk-formulated phage mixtures reduced disease severity on plants compared with the control by 1, 30, 51, and 62%, respectively. In three consecutive field trials, nonformulated phage caused 15, 20, and 9% reduction in disease on treated plants compared with untreated control plants, whereas plants treated with PCF- and Casecrete-formulated phage had 27, 32, and 12% and 30, 43, and 24% disease reduction, respectively. Plants receiving copper-mancozeb treatments were included in two field trials and had a 20% decrease in disease in the first trial and a 13% increase in the second one. Skim milk-formulated phage was tested only once and caused an 18% disease reduction. PCF-formulated phage was more effective when applied in the evening than in the morning, reducing disease on plants by 27 and 13%, respectively. The Casecrete-formulated phage populations were over 1,000-fold higher than the nonformulated phage populations 36 h after phage application.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato
EP  - 954
IS  - 8
SP  - 949
VL  - 87
DO  - 10.1094/PDIS.2003.87.8.949
ER  - 

@article{
author = "Balogh, B and Jones, Jeffrey B. and Momol, Timur M. and Olson, SM and Obradović, Aleksa and King, P and Jackson, LE",
year = "2003",
abstract = "Bacteriophages are currently used as an alternative method for controlling bacterial spot disease on tomato incited by Xanthomonas campestris pv. vesicatoria. However, the efficacy of phage is greatly reduced due to its short residual activity on plant foliage. Three formulations that significantly increased phage longevity on the plant surface were tested in field and greenhouse trials: (i) PCF, 0.5% pregelatinized corn flour (PCF) + 0.5% sucrose; (ii) Casecrete, 0.5% Casecrete NH-400 + 0.5% sucrose + 0.25% PCF; and (iii) skim milk, 0.75% powdered skim milk + 0.5% sucrose. In greenhouse experiments, the nonformulated, PCF-, Casecrete-, and skim milk-formulated phage mixtures reduced disease severity on plants compared with the control by 1, 30, 51, and 62%, respectively. In three consecutive field trials, nonformulated phage caused 15, 20, and 9% reduction in disease on treated plants compared with untreated control plants, whereas plants treated with PCF- and Casecrete-formulated phage had 27, 32, and 12% and 30, 43, and 24% disease reduction, respectively. Plants receiving copper-mancozeb treatments were included in two field trials and had a 20% decrease in disease in the first trial and a 13% increase in the second one. Skim milk-formulated phage was tested only once and caused an 18% disease reduction. PCF-formulated phage was more effective when applied in the evening than in the morning, reducing disease on plants by 27 and 13%, respectively. The Casecrete-formulated phage populations were over 1,000-fold higher than the nonformulated phage populations 36 h after phage application.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato",
pages = "954-949",
number = "8",
volume = "87",
doi = "10.1094/PDIS.2003.87.8.949"
}

Balogh, B., Jones, J. B., Momol, T. M., Olson, S., Obradović, A., King, P.,& Jackson, L.. (2003). Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato. in Plant Disease
Amer Phytopathological Soc, St Paul., 87(8), 949-954.
https://doi.org/10.1094/PDIS.2003.87.8.949

Balogh B, Jones JB, Momol TM, Olson S, Obradović A, King P, Jackson L. Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato. in Plant Disease. 2003;87(8):949-954.
doi:10.1094/PDIS.2003.87.8.949 .

Balogh, B, Jones, Jeffrey B., Momol, Timur M., Olson, SM, Obradović, Aleksa, King, P, Jackson, LE, "Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato" in Plant Disease, 87, no. 8 (2003):949-954,
https://doi.org/10.1094/PDIS.2003.87.8.949 . .

TY  - CONF
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Momol, Timur M.
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/586
AB  - In October 2001, tomato foliage with blighting and a leaf spot was received from a transplant producer in Florida. The seedlings manifested apical or marginal leaf necrosis or discrete lesions along the leaf veins. Non-fluorescent bacterial strains forming viscous, creamy white colonies on King's medium B and causing a hypersensitive reaction in tomato and pepper leaves was consistently isolated from lesions. Pathogenicity was checked on three-week old tomato plants. The plants were incubated in high humidity for 24 h before and after inoculation. Similar symptoms were observed on the inoculated seedlings. We characterised 12 strains using bacteriological tests. According to the fatty acid profile, the strains displayed highest similarity with the bacterium Pseudomonas huttiensis Leifson 1962. Sequence analysis of the 16S rRNA indicated that this bacterium shows 98.7divided by98.8% homology with two Herbaspirillum species. Thus, our strains were compared with one P. huttiensis and five H. rubrisubalbicans strains. The strains from tomato were Gram-negative, non-fluorescent and oxidase positive, but levan, pectate hydrolysis and arginine dihydrolase negative, and grew at 40degreesC. Based on these results, they had a high degree of similarity with both P. huttiensis and H. rubrisubalbicans.
PB  - Springer, Dordrecht
C3  - Pseudomonas Syringae and Related Pathogens: Biology and Genetics
T1  - Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse
EP  - 630
SP  - 627
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_586
ER  - 

@conference{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Minsavage, Gerald V. and Dickstein, Ellen R. and Momol, Timur M.",
year = "2003",
abstract = "In October 2001, tomato foliage with blighting and a leaf spot was received from a transplant producer in Florida. The seedlings manifested apical or marginal leaf necrosis or discrete lesions along the leaf veins. Non-fluorescent bacterial strains forming viscous, creamy white colonies on King's medium B and causing a hypersensitive reaction in tomato and pepper leaves was consistently isolated from lesions. Pathogenicity was checked on three-week old tomato plants. The plants were incubated in high humidity for 24 h before and after inoculation. Similar symptoms were observed on the inoculated seedlings. We characterised 12 strains using bacteriological tests. According to the fatty acid profile, the strains displayed highest similarity with the bacterium Pseudomonas huttiensis Leifson 1962. Sequence analysis of the 16S rRNA indicated that this bacterium shows 98.7divided by98.8% homology with two Herbaspirillum species. Thus, our strains were compared with one P. huttiensis and five H. rubrisubalbicans strains. The strains from tomato were Gram-negative, non-fluorescent and oxidase positive, but levan, pectate hydrolysis and arginine dihydrolase negative, and grew at 40degreesC. Based on these results, they had a high degree of similarity with both P. huttiensis and H. rubrisubalbicans.",
publisher = "Springer, Dordrecht",
journal = "Pseudomonas Syringae and Related Pathogens: Biology and Genetics",
title = "Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse",
pages = "630-627",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_586"
}

Obradović, A., Jones, J. B., Minsavage, G. V., Dickstein, E. R.,& Momol, T. M.. (2003). Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics
Springer, Dordrecht., 627-630.
https://hdl.handle.net/21.15107/rcub_agrospace_586

Obradović A, Jones JB, Minsavage GV, Dickstein ER, Momol TM. Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics. 2003;:627-630.
https://hdl.handle.net/21.15107/rcub_agrospace_586 .

Obradović, Aleksa, Jones, Jeffrey B., Minsavage, Gerald V., Dickstein, Ellen R., Momol, Timur M., "Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse" in Pseudomonas Syringae and Related Pathogens: Biology and Genetics (2003):627-630,
https://hdl.handle.net/21.15107/rcub_agrospace_586 .

TY  - CONF
AU  - Obradović, Aleksa
AU  - Arsenijević, M.
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/585
AB  - In autumn of 1998 and 1999, parsley root rot was observed either in the fields of northern Serbia or during storage. Diseased plants showed total destruction of the leaf rosette and root base resembling soft rot. However, when the remaining part of the root was removed from the soil, reddish brown areas were noticed on the root surface. The tissue was sunken and firm with no cracks visible on the surface. On the root, longitudinal brown discoloration spread from the upper part toward the root tip affecting the vascular cylinder and the surrounding tissue. Thirty-two bacterial isolates were obtained from inner tissue of many roots. All isolates caused a hypersensitive reaction in tobacco leaves within 24 h, were Gram negative, rod-shaped, motile, and produced shiny, greyish-white colonies on King's medium B (KB) without the typical green fluorescent pigment on KB medium in 24 h. They were levan positive but oxidase and arginine dihydrolase negative, non pectolytic, aerobic, utilised sucrose and aesculin, did not reduce nitrates, and did not grow at 37degreesC. Syringomycin and coronatine production was negative and ice nucleation was positive. Based on fatty acid profiles the strains had greatest similarity to Pseudomonas cichorii and P. syringae. Prick-inoculated roots of two-month-old parsley plants caused tissue depression around the point of inoculation and yellowing of older leaves followed by wilting of the foliage one week after inoculation. A week later the roots were pulled out and root rot resembling the symptoms of natural infection was observed. Considering their pathogenic and bacteriological characteristics, investigated bacterial isolates associated with parsley root rot in Serbia, belong to an unusual, non fluorescent Pseudomonas sp.
PB  - Springer, Dordrecht
C3  - Pseudomonas Syringae and Related Pathogens: Biology and Genetics
T1  - An unusual pseudomonad isolated from diseased parsley roots in Serbia
EP  - 634
SP  - 631
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_585
ER  - 

@conference{
author = "Obradović, Aleksa and Arsenijević, M. and Jones, Jeffrey B. and Minsavage, Gerald V.",
year = "2003",
abstract = "In autumn of 1998 and 1999, parsley root rot was observed either in the fields of northern Serbia or during storage. Diseased plants showed total destruction of the leaf rosette and root base resembling soft rot. However, when the remaining part of the root was removed from the soil, reddish brown areas were noticed on the root surface. The tissue was sunken and firm with no cracks visible on the surface. On the root, longitudinal brown discoloration spread from the upper part toward the root tip affecting the vascular cylinder and the surrounding tissue. Thirty-two bacterial isolates were obtained from inner tissue of many roots. All isolates caused a hypersensitive reaction in tobacco leaves within 24 h, were Gram negative, rod-shaped, motile, and produced shiny, greyish-white colonies on King's medium B (KB) without the typical green fluorescent pigment on KB medium in 24 h. They were levan positive but oxidase and arginine dihydrolase negative, non pectolytic, aerobic, utilised sucrose and aesculin, did not reduce nitrates, and did not grow at 37degreesC. Syringomycin and coronatine production was negative and ice nucleation was positive. Based on fatty acid profiles the strains had greatest similarity to Pseudomonas cichorii and P. syringae. Prick-inoculated roots of two-month-old parsley plants caused tissue depression around the point of inoculation and yellowing of older leaves followed by wilting of the foliage one week after inoculation. A week later the roots were pulled out and root rot resembling the symptoms of natural infection was observed. Considering their pathogenic and bacteriological characteristics, investigated bacterial isolates associated with parsley root rot in Serbia, belong to an unusual, non fluorescent Pseudomonas sp.",
publisher = "Springer, Dordrecht",
journal = "Pseudomonas Syringae and Related Pathogens: Biology and Genetics",
title = "An unusual pseudomonad isolated from diseased parsley roots in Serbia",
pages = "634-631",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_585"
}

Obradović, A., Arsenijević, M., Jones, J. B.,& Minsavage, G. V.. (2003). An unusual pseudomonad isolated from diseased parsley roots in Serbia. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics
Springer, Dordrecht., 631-634.
https://hdl.handle.net/21.15107/rcub_agrospace_585

Obradović A, Arsenijević M, Jones JB, Minsavage GV. An unusual pseudomonad isolated from diseased parsley roots in Serbia. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics. 2003;:631-634.
https://hdl.handle.net/21.15107/rcub_agrospace_585 .

Obradović, Aleksa, Arsenijević, M., Jones, Jeffrey B., Minsavage, Gerald V., "An unusual pseudomonad isolated from diseased parsley roots in Serbia" in Pseudomonas Syringae and Related Pathogens: Biology and Genetics (2003):631-634,
https://hdl.handle.net/21.15107/rcub_agrospace_585 .