Minsavage, Gerald V.

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  • Minsavage, Gerald V. (7)
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Author's Bibliography

Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns

Ivanović, Milan; Obradović, Aleksa; Gašić, Katarina; Minsavage, Gerald V.; Dickstein, Ellen R.; Jones, Jeffrey B.

(Springer, Dordrecht, 2012)

TY  - JOUR
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3017
AB  - Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that the E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns
EP  - 557
IS  - 3
SP  - 545
VL  - 133
DO  - 10.1007/s10658-011-9926-8
ER  - 
@article{
author = "Ivanović, Milan and Obradović, Aleksa and Gašić, Katarina and Minsavage, Gerald V. and Dickstein, Ellen R. and Jones, Jeffrey B.",
year = "2012",
abstract = "Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that the E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns",
pages = "557-545",
number = "3",
volume = "133",
doi = "10.1007/s10658-011-9926-8"
}
Ivanović, M., Obradović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns. in European Journal of Plant Pathology
Springer, Dordrecht., 133(3), 545-557.
https://doi.org/10.1007/s10658-011-9926-8
Ivanović M, Obradović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns. in European Journal of Plant Pathology. 2012;133(3):545-557.
doi:10.1007/s10658-011-9926-8 .
Ivanović, Milan, Obradović, Aleksa, Gašić, Katarina, Minsavage, Gerald V., Dickstein, Ellen R., Jones, Jeffrey B., "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniquesand detection of new PFGE patterns" in European Journal of Plant Pathology, 133, no. 3 (2012):545-557,
https://doi.org/10.1007/s10658-011-9926-8 . .
4
6
5

Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns

Ivanović, Milan; Obadović, Aleksa; Gašić, Katarina; Minsavage, Gerald V.; Dickstein, Ellen R.; Jones, Jeffrey B.

(Springer, Dordrecht, 2012)

TY  - JOUR
AU  - Ivanović, Milan
AU  - Obadović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3016
AB  - Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns
EP  - 727
IS  - 3
SP  - 715
VL  - 133
DO  - 10.1007/s10658-012-9950-3
ER  - 
@article{
author = "Ivanović, Milan and Obadović, Aleksa and Gašić, Katarina and Minsavage, Gerald V. and Dickstein, Ellen R. and Jones, Jeffrey B.",
year = "2012",
abstract = "Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns",
pages = "727-715",
number = "3",
volume = "133",
doi = "10.1007/s10658-012-9950-3"
}
Ivanović, M., Obadović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns. in European Journal of Plant Pathology
Springer, Dordrecht., 133(3), 715-727.
https://doi.org/10.1007/s10658-012-9950-3
Ivanović M, Obadović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns. in European Journal of Plant Pathology. 2012;133(3):715-727.
doi:10.1007/s10658-012-9950-3 .
Ivanović, Milan, Obadović, Aleksa, Gašić, Katarina, Minsavage, Gerald V., Dickstein, Ellen R., Jones, Jeffrey B., "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns" in European Journal of Plant Pathology, 133, no. 3 (2012):715-727,
https://doi.org/10.1007/s10658-012-9950-3 . .
5
3
5

Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)

Ivanović, Milan; Obradović, Aleksa; Gašić, Katarina; Minsavage, Gerald V.; Dickstein, Ellen R.; Jones, Jeffrey B.

(Springer, Dordrecht, 2012)

TY  - JOUR
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Jones, Jeffrey B.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3008
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)
EP  - 443
IS  - 2
SP  - 443
VL  - 134
DO  - 10.1007/s10658-012-0037-y
ER  - 
@article{
author = "Ivanović, Milan and Obradović, Aleksa and Gašić, Katarina and Minsavage, Gerald V. and Dickstein, Ellen R. and Jones, Jeffrey B.",
year = "2012",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)",
pages = "443-443",
number = "2",
volume = "134",
doi = "10.1007/s10658-012-0037-y"
}
Ivanović, M., Obradović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012). in European Journal of Plant Pathology
Springer, Dordrecht., 134(2), 443-443.
https://doi.org/10.1007/s10658-012-0037-y
Ivanović M, Obradović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012). in European Journal of Plant Pathology. 2012;134(2):443-443.
doi:10.1007/s10658-012-0037-y .
Ivanović, Milan, Obradović, Aleksa, Gašić, Katarina, Minsavage, Gerald V., Dickstein, Ellen R., Jones, Jeffrey B., "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)" in European Journal of Plant Pathology, 134, no. 2 (2012):443-443,
https://doi.org/10.1007/s10658-012-0037-y . .

A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.

Obradović, Aleksa; Jones, Jeffrey B.; Minsavage, Gerald V.; Dickstein, Ellen R.; Momol, Timur M.

(Amer Phytopathological Soc, St Paul, 2007)

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Momol, Timur M.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1499
AB  - A leaf spot and blighting were observed on leaves of tomato transplants from a producer in Florida in 2001 and 2002. A nonfluorescent bacterium was isolated consistently from affected tissue. The typical bacterium was a gram negative, strictly aerobic, slightly curved rod with one or two flagella. Sequence analysis of the 16S rRNA indicated that two representative strains, F1 and SE1, had greater than 99% nucleotide sequence identity with Herbaspirillum huttiense and H. rubrisubalbicans. The cellular fatty acid composition of the total of 16 tomato strains was very similar to H. huttiense and H. rubrisubalbicans. Based on carbon utilization, six of nine strains tested with the Biolog system were identified as Herbaspirillum spp. The tomato strains were oxidase positive and grew at 40 degrees C, but were negative for levan production, pectate hydrolysis, and arginine dihydrolase activity. Based upon this polyphasic analysis, we concluded that the strains were most closely related to H. huttiense, although placement in this species would require further analyses. However, the tomato strains and H. rubrisubalbicans, but not H. huttiense, caused confluent necrosis when infiltrated at high concentrations into tomato leaves and were able to produce leaf spot symptoms on inoculated tomato seedlings in the greenhouse. Using pulsed-field gel electrophoresis, we determined that there was considerable variability between the strains collected in 2001 and 2002.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.
EP  - 890
IS  - 7
SP  - 886
VL  - 91
DO  - 10.1094/PDIS-91-7-0886
ER  - 
@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Minsavage, Gerald V. and Dickstein, Ellen R. and Momol, Timur M.",
year = "2007",
abstract = "A leaf spot and blighting were observed on leaves of tomato transplants from a producer in Florida in 2001 and 2002. A nonfluorescent bacterium was isolated consistently from affected tissue. The typical bacterium was a gram negative, strictly aerobic, slightly curved rod with one or two flagella. Sequence analysis of the 16S rRNA indicated that two representative strains, F1 and SE1, had greater than 99% nucleotide sequence identity with Herbaspirillum huttiense and H. rubrisubalbicans. The cellular fatty acid composition of the total of 16 tomato strains was very similar to H. huttiense and H. rubrisubalbicans. Based on carbon utilization, six of nine strains tested with the Biolog system were identified as Herbaspirillum spp. The tomato strains were oxidase positive and grew at 40 degrees C, but were negative for levan production, pectate hydrolysis, and arginine dihydrolase activity. Based upon this polyphasic analysis, we concluded that the strains were most closely related to H. huttiense, although placement in this species would require further analyses. However, the tomato strains and H. rubrisubalbicans, but not H. huttiense, caused confluent necrosis when infiltrated at high concentrations into tomato leaves and were able to produce leaf spot symptoms on inoculated tomato seedlings in the greenhouse. Using pulsed-field gel electrophoresis, we determined that there was considerable variability between the strains collected in 2001 and 2002.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.",
pages = "890-886",
number = "7",
volume = "91",
doi = "10.1094/PDIS-91-7-0886"
}
Obradović, A., Jones, J. B., Minsavage, G. V., Dickstein, E. R.,& Momol, T. M.. (2007). A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.. in Plant Disease
Amer Phytopathological Soc, St Paul., 91(7), 886-890.
https://doi.org/10.1094/PDIS-91-7-0886
Obradović A, Jones JB, Minsavage GV, Dickstein ER, Momol TM. A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.. in Plant Disease. 2007;91(7):886-890.
doi:10.1094/PDIS-91-7-0886 .
Obradović, Aleksa, Jones, Jeffrey B., Minsavage, Gerald V., Dickstein, Ellen R., Momol, Timur M., "A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp." in Plant Disease, 91, no. 7 (2007):886-890,
https://doi.org/10.1094/PDIS-91-7-0886 . .
5
3

Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia

Obradović, Aleksa; Mavridis, A; Rudolph, K; Janse, JD; Arsenijević, M.; Jones, Jeffrey B.; Minsavage, Gerald V.; Wang, JF

(Springer, Dordrecht, 2004)

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Mavridis, A
AU  - Rudolph, K
AU  - Janse, JD
AU  - Arsenijević, M.
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Wang, JF
PY  - 2004
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/820
AB  - During the last two decades bacterial strains associated with necrotic leaf spots of pepper and tomato fruit spots were collected in Serbia. Twenty-eight strains isolated from pepper and six from tomato were characterized. A study of their physiological and pathological characteristics, and fatty acid composition analysis revealed that all of the strains belong to Xanthomonas campestris pv. vesicatoria. Being non-amylolytic and non-pectolytic, pathogenic on pepper but not on tomato, containing lower amounts of fatty acid 15 : 0 ante-iso, the pepper strains were designated as members of the A group of X. campestris pv. vesicatoria. However, the tomato strains hydrolyzed starch and pectate, caused compatible reactions on tomato but not on pepper, had higher percent of 15 : 0 ante iso fatty acid, and were classified into B phenotypic group and identified as X. vesicatoria. PCR primers were developed which amplified conserved DNA regions related to the hrp genes of different strains of X. campestris pv. vesicatoria associated with pepper and tomato. Restriction analysis of the PCR product resulted in different patterns and enabled grouping of the strains into four groups. When xanthomonads isolated from pepper and tomato in Serbia were analyzed, they clustered into two groups corresponding to the grouping based on their physiological and pathological characteristics. According to the reaction of pepper and tomato differential varieties, the strains from pepper belong to races P7 and P8 and tomato strains belong to the race T2. All strains were sensitive to copper and streptomycin. Advantages and disadvantages of various bacterial spot management practices are discussed.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia
EP  - 292
IS  - 3
SP  - 285
VL  - 110
DO  - 10.1023/B:EJPP.0000019797.27952.1d
ER  - 
@article{
author = "Obradović, Aleksa and Mavridis, A and Rudolph, K and Janse, JD and Arsenijević, M. and Jones, Jeffrey B. and Minsavage, Gerald V. and Wang, JF",
year = "2004",
abstract = "During the last two decades bacterial strains associated with necrotic leaf spots of pepper and tomato fruit spots were collected in Serbia. Twenty-eight strains isolated from pepper and six from tomato were characterized. A study of their physiological and pathological characteristics, and fatty acid composition analysis revealed that all of the strains belong to Xanthomonas campestris pv. vesicatoria. Being non-amylolytic and non-pectolytic, pathogenic on pepper but not on tomato, containing lower amounts of fatty acid 15 : 0 ante-iso, the pepper strains were designated as members of the A group of X. campestris pv. vesicatoria. However, the tomato strains hydrolyzed starch and pectate, caused compatible reactions on tomato but not on pepper, had higher percent of 15 : 0 ante iso fatty acid, and were classified into B phenotypic group and identified as X. vesicatoria. PCR primers were developed which amplified conserved DNA regions related to the hrp genes of different strains of X. campestris pv. vesicatoria associated with pepper and tomato. Restriction analysis of the PCR product resulted in different patterns and enabled grouping of the strains into four groups. When xanthomonads isolated from pepper and tomato in Serbia were analyzed, they clustered into two groups corresponding to the grouping based on their physiological and pathological characteristics. According to the reaction of pepper and tomato differential varieties, the strains from pepper belong to races P7 and P8 and tomato strains belong to the race T2. All strains were sensitive to copper and streptomycin. Advantages and disadvantages of various bacterial spot management practices are discussed.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia",
pages = "292-285",
number = "3",
volume = "110",
doi = "10.1023/B:EJPP.0000019797.27952.1d"
}
Obradović, A., Mavridis, A., Rudolph, K., Janse, J., Arsenijević, M., Jones, J. B., Minsavage, G. V.,& Wang, J.. (2004). Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 110(3), 285-292.
https://doi.org/10.1023/B:EJPP.0000019797.27952.1d
Obradović A, Mavridis A, Rudolph K, Janse J, Arsenijević M, Jones JB, Minsavage GV, Wang J. Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. in European Journal of Plant Pathology. 2004;110(3):285-292.
doi:10.1023/B:EJPP.0000019797.27952.1d .
Obradović, Aleksa, Mavridis, A, Rudolph, K, Janse, JD, Arsenijević, M., Jones, Jeffrey B., Minsavage, Gerald V., Wang, JF, "Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia" in European Journal of Plant Pathology, 110, no. 3 (2004):285-292,
https://doi.org/10.1023/B:EJPP.0000019797.27952.1d . .
69
53
64

Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse

Obradović, Aleksa; Jones, Jeffrey B.; Minsavage, Gerald V.; Dickstein, Ellen R.; Momol, Timur M.

(Springer, Dordrecht, 2003)

TY  - CONF
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Momol, Timur M.
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/586
AB  - In October 2001, tomato foliage with blighting and a leaf spot was received from a transplant producer in Florida. The seedlings manifested apical or marginal leaf necrosis or discrete lesions along the leaf veins. Non-fluorescent bacterial strains forming viscous, creamy white colonies on King's medium B and causing a hypersensitive reaction in tomato and pepper leaves was consistently isolated from lesions. Pathogenicity was checked on three-week old tomato plants. The plants were incubated in high humidity for 24 h before and after inoculation. Similar symptoms were observed on the inoculated seedlings. We characterised 12 strains using bacteriological tests. According to the fatty acid profile, the strains displayed highest similarity with the bacterium Pseudomonas huttiensis Leifson 1962. Sequence analysis of the 16S rRNA indicated that this bacterium shows 98.7divided by98.8% homology with two Herbaspirillum species. Thus, our strains were compared with one P. huttiensis and five H. rubrisubalbicans strains. The strains from tomato were Gram-negative, non-fluorescent and oxidase positive, but levan, pectate hydrolysis and arginine dihydrolase negative, and grew at 40degreesC. Based on these results, they had a high degree of similarity with both P. huttiensis and H. rubrisubalbicans.
PB  - Springer, Dordrecht
C3  - Pseudomonas Syringae and Related Pathogens: Biology and Genetics
T1  - Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse
EP  - 630
SP  - 627
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_586
ER  - 
@conference{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Minsavage, Gerald V. and Dickstein, Ellen R. and Momol, Timur M.",
year = "2003",
abstract = "In October 2001, tomato foliage with blighting and a leaf spot was received from a transplant producer in Florida. The seedlings manifested apical or marginal leaf necrosis or discrete lesions along the leaf veins. Non-fluorescent bacterial strains forming viscous, creamy white colonies on King's medium B and causing a hypersensitive reaction in tomato and pepper leaves was consistently isolated from lesions. Pathogenicity was checked on three-week old tomato plants. The plants were incubated in high humidity for 24 h before and after inoculation. Similar symptoms were observed on the inoculated seedlings. We characterised 12 strains using bacteriological tests. According to the fatty acid profile, the strains displayed highest similarity with the bacterium Pseudomonas huttiensis Leifson 1962. Sequence analysis of the 16S rRNA indicated that this bacterium shows 98.7divided by98.8% homology with two Herbaspirillum species. Thus, our strains were compared with one P. huttiensis and five H. rubrisubalbicans strains. The strains from tomato were Gram-negative, non-fluorescent and oxidase positive, but levan, pectate hydrolysis and arginine dihydrolase negative, and grew at 40degreesC. Based on these results, they had a high degree of similarity with both P. huttiensis and H. rubrisubalbicans.",
publisher = "Springer, Dordrecht",
journal = "Pseudomonas Syringae and Related Pathogens: Biology and Genetics",
title = "Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse",
pages = "630-627",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_586"
}
Obradović, A., Jones, J. B., Minsavage, G. V., Dickstein, E. R.,& Momol, T. M.. (2003). Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics
Springer, Dordrecht., 627-630.
https://hdl.handle.net/21.15107/rcub_agrospace_586
Obradović A, Jones JB, Minsavage GV, Dickstein ER, Momol TM. Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics. 2003;:627-630.
https://hdl.handle.net/21.15107/rcub_agrospace_586 .
Obradović, Aleksa, Jones, Jeffrey B., Minsavage, Gerald V., Dickstein, Ellen R., Momol, Timur M., "Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse" in Pseudomonas Syringae and Related Pathogens: Biology and Genetics (2003):627-630,
https://hdl.handle.net/21.15107/rcub_agrospace_586 .

An unusual pseudomonad isolated from diseased parsley roots in Serbia

Obradović, Aleksa; Arsenijević, M.; Jones, Jeffrey B.; Minsavage, Gerald V.

(Springer, Dordrecht, 2003)

TY  - CONF
AU  - Obradović, Aleksa
AU  - Arsenijević, M.
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/585
AB  - In autumn of 1998 and 1999, parsley root rot was observed either in the fields of northern Serbia or during storage. Diseased plants showed total destruction of the leaf rosette and root base resembling soft rot. However, when the remaining part of the root was removed from the soil, reddish brown areas were noticed on the root surface. The tissue was sunken and firm with no cracks visible on the surface. On the root, longitudinal brown discoloration spread from the upper part toward the root tip affecting the vascular cylinder and the surrounding tissue. Thirty-two bacterial isolates were obtained from inner tissue of many roots. All isolates caused a hypersensitive reaction in tobacco leaves within 24 h, were Gram negative, rod-shaped, motile, and produced shiny, greyish-white colonies on King's medium B (KB) without the typical green fluorescent pigment on KB medium in 24 h. They were levan positive but oxidase and arginine dihydrolase negative, non pectolytic, aerobic, utilised sucrose and aesculin, did not reduce nitrates, and did not grow at 37degreesC. Syringomycin and coronatine production was negative and ice nucleation was positive. Based on fatty acid profiles the strains had greatest similarity to Pseudomonas cichorii and P. syringae. Prick-inoculated roots of two-month-old parsley plants caused tissue depression around the point of inoculation and yellowing of older leaves followed by wilting of the foliage one week after inoculation. A week later the roots were pulled out and root rot resembling the symptoms of natural infection was observed. Considering their pathogenic and bacteriological characteristics, investigated bacterial isolates associated with parsley root rot in Serbia, belong to an unusual, non fluorescent Pseudomonas sp.
PB  - Springer, Dordrecht
C3  - Pseudomonas Syringae and Related Pathogens: Biology and Genetics
T1  - An unusual pseudomonad isolated from diseased parsley roots in Serbia
EP  - 634
SP  - 631
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_585
ER  - 
@conference{
author = "Obradović, Aleksa and Arsenijević, M. and Jones, Jeffrey B. and Minsavage, Gerald V.",
year = "2003",
abstract = "In autumn of 1998 and 1999, parsley root rot was observed either in the fields of northern Serbia or during storage. Diseased plants showed total destruction of the leaf rosette and root base resembling soft rot. However, when the remaining part of the root was removed from the soil, reddish brown areas were noticed on the root surface. The tissue was sunken and firm with no cracks visible on the surface. On the root, longitudinal brown discoloration spread from the upper part toward the root tip affecting the vascular cylinder and the surrounding tissue. Thirty-two bacterial isolates were obtained from inner tissue of many roots. All isolates caused a hypersensitive reaction in tobacco leaves within 24 h, were Gram negative, rod-shaped, motile, and produced shiny, greyish-white colonies on King's medium B (KB) without the typical green fluorescent pigment on KB medium in 24 h. They were levan positive but oxidase and arginine dihydrolase negative, non pectolytic, aerobic, utilised sucrose and aesculin, did not reduce nitrates, and did not grow at 37degreesC. Syringomycin and coronatine production was negative and ice nucleation was positive. Based on fatty acid profiles the strains had greatest similarity to Pseudomonas cichorii and P. syringae. Prick-inoculated roots of two-month-old parsley plants caused tissue depression around the point of inoculation and yellowing of older leaves followed by wilting of the foliage one week after inoculation. A week later the roots were pulled out and root rot resembling the symptoms of natural infection was observed. Considering their pathogenic and bacteriological characteristics, investigated bacterial isolates associated with parsley root rot in Serbia, belong to an unusual, non fluorescent Pseudomonas sp.",
publisher = "Springer, Dordrecht",
journal = "Pseudomonas Syringae and Related Pathogens: Biology and Genetics",
title = "An unusual pseudomonad isolated from diseased parsley roots in Serbia",
pages = "634-631",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_585"
}
Obradović, A., Arsenijević, M., Jones, J. B.,& Minsavage, G. V.. (2003). An unusual pseudomonad isolated from diseased parsley roots in Serbia. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics
Springer, Dordrecht., 631-634.
https://hdl.handle.net/21.15107/rcub_agrospace_585
Obradović A, Arsenijević M, Jones JB, Minsavage GV. An unusual pseudomonad isolated from diseased parsley roots in Serbia. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics. 2003;:631-634.
https://hdl.handle.net/21.15107/rcub_agrospace_585 .
Obradović, Aleksa, Arsenijević, M., Jones, Jeffrey B., Minsavage, Gerald V., "An unusual pseudomonad isolated from diseased parsley roots in Serbia" in Pseudomonas Syringae and Related Pathogens: Biology and Genetics (2003):631-634,
https://hdl.handle.net/21.15107/rcub_agrospace_585 .