TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Balogh, B.
AU  - Momol, Timur M.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1846
AB  - Tomato bacterial spot, caused by Xanthomonas spp. complex, is a constant threat to tomato (Solanum lycopersicum) commercial production, but is especially severe in Florida and the southeastern US when weather conditions become conducive for disease development. In an effort to develop more sustainable strategies for reducing severity of the disease, we investigated various combinations of PGPR strains, bacterial antagonists, bacteriophages and SAR inducers (harpin, acibenzolar-S-methyl) in greenhouse and field trials. The idea was to search for alternative treatments that could be integrated with conventional practices, in order to improve disease control and yield responses. After screening single treatments and their combinations for efficacy in a series of greenhouse experiments, acibenzolar-S-methyl (ASM) and phage treatment provided the most promising results. In field trials, carried out in three consecutive seasons, ASM significantly reduced disease severity compared to the untreated control. However, the combination of ASM and formulated host-specific phages provided an additional reduction in disease pressure and resulted in more efficient foliar disease control than ASM, phage, or copper-macozeb alone. Integrated application of phages, ASM and other practices is currently widely used in greenhouses and production fields in Florida as a part of a standard integrated management strategy for tomato bacterial spot control. http://www.actahort.org/books/808/index.htm
T2  - Acta Horticulturae
T1  - Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice
EP  - 346
SP  - 343
VL  - 808
DO  - 10.17660/ActaHortic.2009.808.55
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Balogh, B. and Momol, Timur M.",
year = "2009",
abstract = "Tomato bacterial spot, caused by Xanthomonas spp. complex, is a constant threat to tomato (Solanum lycopersicum) commercial production, but is especially severe in Florida and the southeastern US when weather conditions become conducive for disease development. In an effort to develop more sustainable strategies for reducing severity of the disease, we investigated various combinations of PGPR strains, bacterial antagonists, bacteriophages and SAR inducers (harpin, acibenzolar-S-methyl) in greenhouse and field trials. The idea was to search for alternative treatments that could be integrated with conventional practices, in order to improve disease control and yield responses. After screening single treatments and their combinations for efficacy in a series of greenhouse experiments, acibenzolar-S-methyl (ASM) and phage treatment provided the most promising results. In field trials, carried out in three consecutive seasons, ASM significantly reduced disease severity compared to the untreated control. However, the combination of ASM and formulated host-specific phages provided an additional reduction in disease pressure and resulted in more efficient foliar disease control than ASM, phage, or copper-macozeb alone. Integrated application of phages, ASM and other practices is currently widely used in greenhouses and production fields in Florida as a part of a standard integrated management strategy for tomato bacterial spot control. http://www.actahort.org/books/808/index.htm",
journal = "Acta Horticulturae",
title = "Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice",
pages = "346-343",
volume = "808",
doi = "10.17660/ActaHortic.2009.808.55"
}

Obradović, A., Jones, J. B., Balogh, B.,& Momol, T. M.. (2009). Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice. in Acta Horticulturae, 808, 343-346.
https://doi.org/10.17660/ActaHortic.2009.808.55

Obradović A, Jones JB, Balogh B, Momol TM. Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice. in Acta Horticulturae. 2009;808:343-346.
doi:10.17660/ActaHortic.2009.808.55 .

Obradović, Aleksa, Jones, Jeffrey B., Balogh, B., Momol, Timur M., "Development of an Integrated Management of Tomato Bacterial Spot - A Strategy That Lives in Practice" in Acta Horticulturae, 808 (2009):343-346,
https://doi.org/10.17660/ActaHortic.2009.808.55 . .

TY  - CHAP
AU  - Balogh, B.
AU  - Momol, Timur M.
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1843
T2  - Disease Control in Crops: Biological and Environmentally-Friendly Approaches
T1  - Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria
EP  - 256
SP  - 246
DO  - 10.1002/9781444312157.ch13
ER  - 

@inbook{
author = "Balogh, B. and Momol, Timur M. and Obradović, Aleksa and Jones, Jeffrey B.",
year = "2009",
journal = "Disease Control in Crops: Biological and Environmentally-Friendly Approaches",
booktitle = "Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria",
pages = "256-246",
doi = "10.1002/9781444312157.ch13"
}

Balogh, B., Momol, T. M., Obradović, A.,& Jones, J. B.. (2009). Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria. in Disease Control in Crops: Biological and Environmentally-Friendly Approaches, 246-256.
https://doi.org/10.1002/9781444312157.ch13

Balogh B, Momol TM, Obradović A, Jones JB. Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria. in Disease Control in Crops: Biological and Environmentally-Friendly Approaches. 2009;:246-256.
doi:10.1002/9781444312157.ch13 .

Balogh, B., Momol, Timur M., Obradović, Aleksa, Jones, Jeffrey B., "Bacteriophages as Agents for the Control of Plant Pathogenic Bacteria" in Disease Control in Crops: Biological and Environmentally-Friendly Approaches (2009):246-256,
https://doi.org/10.1002/9781444312157.ch13 . .

TY  - JOUR
AU  - Jones, Jeffrey B.
AU  - Jackson, LE
AU  - Balogh, B.
AU  - Obradović, Aleksa
AU  - Iriarte, FB
AU  - Momol, Timur M.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1598
AB  - The use of phages for disease control is a fast expanding area of plant protection with great potential to replace the chemical control measures now prevalent. Phages can be used effectively as part of integrated disease management strategies. The relative ease of preparing phage treatments and low cost of production of these agents make them good candidates for widespread use in developing countries as well. However, the efficacy of phages, as is true of many biological control agents, depends greatly on prevailing environmental factors as well as on susceptibility of the target organism. Great care is necessary during development, production and application of phage treatments. In addition, constant monitoring for the emergence of resistant bacterial strains is essential. Phage-based disease control management is a dynamic process with a need for continuous adjustment of the phage preparation in order to effectively fight potentially adapting pathogenic bacteria.
PB  - Annual Reviews, Palo Alto
T2  - Annual Review of Phytopathology
T1  - Bacteriophages for plant disease control
EP  - 262
SP  - 245
VL  - 45
DO  - 10.1146/annurev.phyto.45.062806.094411
ER  - 

@article{
author = "Jones, Jeffrey B. and Jackson, LE and Balogh, B. and Obradović, Aleksa and Iriarte, FB and Momol, Timur M.",
year = "2007",
abstract = "The use of phages for disease control is a fast expanding area of plant protection with great potential to replace the chemical control measures now prevalent. Phages can be used effectively as part of integrated disease management strategies. The relative ease of preparing phage treatments and low cost of production of these agents make them good candidates for widespread use in developing countries as well. However, the efficacy of phages, as is true of many biological control agents, depends greatly on prevailing environmental factors as well as on susceptibility of the target organism. Great care is necessary during development, production and application of phage treatments. In addition, constant monitoring for the emergence of resistant bacterial strains is essential. Phage-based disease control management is a dynamic process with a need for continuous adjustment of the phage preparation in order to effectively fight potentially adapting pathogenic bacteria.",
publisher = "Annual Reviews, Palo Alto",
journal = "Annual Review of Phytopathology",
title = "Bacteriophages for plant disease control",
pages = "262-245",
volume = "45",
doi = "10.1146/annurev.phyto.45.062806.094411"
}

Jones, J. B., Jackson, L., Balogh, B., Obradović, A., Iriarte, F.,& Momol, T. M.. (2007). Bacteriophages for plant disease control. in Annual Review of Phytopathology
Annual Reviews, Palo Alto., 45, 245-262.
https://doi.org/10.1146/annurev.phyto.45.062806.094411

Jones JB, Jackson L, Balogh B, Obradović A, Iriarte F, Momol TM. Bacteriophages for plant disease control. in Annual Review of Phytopathology. 2007;45:245-262.
doi:10.1146/annurev.phyto.45.062806.094411 .

Jones, Jeffrey B., Jackson, LE, Balogh, B., Obradović, Aleksa, Iriarte, FB, Momol, Timur M., "Bacteriophages for plant disease control" in Annual Review of Phytopathology, 45 (2007):245-262,
https://doi.org/10.1146/annurev.phyto.45.062806.094411 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Momol, Timur M.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1499
AB  - A leaf spot and blighting were observed on leaves of tomato transplants from a producer in Florida in 2001 and 2002. A nonfluorescent bacterium was isolated consistently from affected tissue. The typical bacterium was a gram negative, strictly aerobic, slightly curved rod with one or two flagella. Sequence analysis of the 16S rRNA indicated that two representative strains, F1 and SE1, had greater than 99% nucleotide sequence identity with Herbaspirillum huttiense and H. rubrisubalbicans. The cellular fatty acid composition of the total of 16 tomato strains was very similar to H. huttiense and H. rubrisubalbicans. Based on carbon utilization, six of nine strains tested with the Biolog system were identified as Herbaspirillum spp. The tomato strains were oxidase positive and grew at 40 degrees C, but were negative for levan production, pectate hydrolysis, and arginine dihydrolase activity. Based upon this polyphasic analysis, we concluded that the strains were most closely related to H. huttiense, although placement in this species would require further analyses. However, the tomato strains and H. rubrisubalbicans, but not H. huttiense, caused confluent necrosis when infiltrated at high concentrations into tomato leaves and were able to produce leaf spot symptoms on inoculated tomato seedlings in the greenhouse. Using pulsed-field gel electrophoresis, we determined that there was considerable variability between the strains collected in 2001 and 2002.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.
EP  - 890
IS  - 7
SP  - 886
VL  - 91
DO  - 10.1094/PDIS-91-7-0886
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Minsavage, Gerald V. and Dickstein, Ellen R. and Momol, Timur M.",
year = "2007",
abstract = "A leaf spot and blighting were observed on leaves of tomato transplants from a producer in Florida in 2001 and 2002. A nonfluorescent bacterium was isolated consistently from affected tissue. The typical bacterium was a gram negative, strictly aerobic, slightly curved rod with one or two flagella. Sequence analysis of the 16S rRNA indicated that two representative strains, F1 and SE1, had greater than 99% nucleotide sequence identity with Herbaspirillum huttiense and H. rubrisubalbicans. The cellular fatty acid composition of the total of 16 tomato strains was very similar to H. huttiense and H. rubrisubalbicans. Based on carbon utilization, six of nine strains tested with the Biolog system were identified as Herbaspirillum spp. The tomato strains were oxidase positive and grew at 40 degrees C, but were negative for levan production, pectate hydrolysis, and arginine dihydrolase activity. Based upon this polyphasic analysis, we concluded that the strains were most closely related to H. huttiense, although placement in this species would require further analyses. However, the tomato strains and H. rubrisubalbicans, but not H. huttiense, caused confluent necrosis when infiltrated at high concentrations into tomato leaves and were able to produce leaf spot symptoms on inoculated tomato seedlings in the greenhouse. Using pulsed-field gel electrophoresis, we determined that there was considerable variability between the strains collected in 2001 and 2002.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.",
pages = "890-886",
number = "7",
volume = "91",
doi = "10.1094/PDIS-91-7-0886"
}

Obradović, A., Jones, J. B., Minsavage, G. V., Dickstein, E. R.,& Momol, T. M.. (2007). A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.. in Plant Disease
Amer Phytopathological Soc, St Paul., 91(7), 886-890.
https://doi.org/10.1094/PDIS-91-7-0886

Obradović A, Jones JB, Minsavage GV, Dickstein ER, Momol TM. A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp.. in Plant Disease. 2007;91(7):886-890.
doi:10.1094/PDIS-91-7-0886 .

Obradović, Aleksa, Jones, Jeffrey B., Minsavage, Gerald V., Dickstein, Ellen R., Momol, Timur M., "A leaf spot and blight of greenhouse tomato seedlings incited by a Herbaspirillum sp." in Plant Disease, 91, no. 7 (2007):886-890,
https://doi.org/10.1094/PDIS-91-7-0886 . .

TY  - CONF
AU  - Balogh, B.
AU  - Iriarte, FB
AU  - Obradović, Aleksa
AU  - Momol, Timur M.
AU  - Jones, Jeffrey B.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1490
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Phages don't have it easy.
EP  - S141
IS  - 7
SP  - S141
VL  - 97
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1490
ER  - 

@conference{
author = "Balogh, B. and Iriarte, FB and Obradović, Aleksa and Momol, Timur M. and Jones, Jeffrey B.",
year = "2007",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Phages don't have it easy.",
pages = "S141-S141",
number = "7",
volume = "97",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1490"
}

Balogh, B., Iriarte, F., Obradović, A., Momol, T. M.,& Jones, J. B.. (2007). Phages don't have it easy.. in Phytopathology
Amer Phytopathological Soc, St Paul., 97(7), S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_1490

Balogh B, Iriarte F, Obradović A, Momol TM, Jones JB. Phages don't have it easy.. in Phytopathology. 2007;97(7):S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_1490 .

Balogh, B., Iriarte, FB, Obradović, Aleksa, Momol, Timur M., Jones, Jeffrey B., "Phages don't have it easy." in Phytopathology, 97, no. 7 (2007):S141-S141,
https://hdl.handle.net/21.15107/rcub_agrospace_1490 .

TY  - CONF
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Obradović, Aleksa
AU  - Balogh, B
AU  - Olson, SM
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1110
AB  - Bacterial spot of tomato, incited by Xanthomonas campestris pv. vesicatoria, is a perennial problem in commercial fields in Florida. Various strategies have been used to control this disease including applying copper bactericides, antibiotics and various other chemicals. Although copper compounds and streptomycin were initially effective in disease control, the presence of strains resistant to these compounds has reduced the efficacy of these compounds. Systemic acquired resistance (SAR) compounds have become a popular alternative to conventional bactericides. One that has shown promise for bacterial spot control is acibenzolar-S-methyl (ASM). Other SAR compounds tested have had variable results. A second strategy which has excellent promise is the application of bacteriophages specific to the bacterial spot pathogen. Following the development of an application strategy, which included a modification in formulation and the timing of bacteriophage applications, bacteriophages were improved in efficacy. We have conducted studies using various combinations of bacteriophages and SAR compounds. In three consecutive seasons, ASM applied in combination with bacteriophage significantly reduced bacterial spot compared to the other treatments. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared to the results of the corresponding non-bacteriophage group, the former provided significantly better disease control and yield of total marketable fruits. Although ASM applications controlled bacterial spot,yield was not significantly improved compared to plots receiving no ASM applications. Bacteriophages offer an excellent alternative to conventional disease control strategies and combining it with ASM shows promise.
PB  - International Society Horticultural Science, Leuven 1
C3  - Proceedings of the 1st International Symposium on Tomato Diseases
T1  - Bacterial spot management on tomatoes
EP  - 124
IS  - 695
SP  - 119
DO  - 10.17660/ActaHortic.2005.695.13
ER  - 

@conference{
author = "Jones, Jeffrey B. and Momol, Timur M. and Obradović, Aleksa and Balogh, B and Olson, SM",
year = "2005",
abstract = "Bacterial spot of tomato, incited by Xanthomonas campestris pv. vesicatoria, is a perennial problem in commercial fields in Florida. Various strategies have been used to control this disease including applying copper bactericides, antibiotics and various other chemicals. Although copper compounds and streptomycin were initially effective in disease control, the presence of strains resistant to these compounds has reduced the efficacy of these compounds. Systemic acquired resistance (SAR) compounds have become a popular alternative to conventional bactericides. One that has shown promise for bacterial spot control is acibenzolar-S-methyl (ASM). Other SAR compounds tested have had variable results. A second strategy which has excellent promise is the application of bacteriophages specific to the bacterial spot pathogen. Following the development of an application strategy, which included a modification in formulation and the timing of bacteriophage applications, bacteriophages were improved in efficacy. We have conducted studies using various combinations of bacteriophages and SAR compounds. In three consecutive seasons, ASM applied in combination with bacteriophage significantly reduced bacterial spot compared to the other treatments. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared to the results of the corresponding non-bacteriophage group, the former provided significantly better disease control and yield of total marketable fruits. Although ASM applications controlled bacterial spot,yield was not significantly improved compared to plots receiving no ASM applications. Bacteriophages offer an excellent alternative to conventional disease control strategies and combining it with ASM shows promise.",
publisher = "International Society Horticultural Science, Leuven 1",
journal = "Proceedings of the 1st International Symposium on Tomato Diseases",
title = "Bacterial spot management on tomatoes",
pages = "124-119",
number = "695",
doi = "10.17660/ActaHortic.2005.695.13"
}

Jones, J. B., Momol, T. M., Obradović, A., Balogh, B.,& Olson, S.. (2005). Bacterial spot management on tomatoes. in Proceedings of the 1st International Symposium on Tomato Diseases
International Society Horticultural Science, Leuven 1.(695), 119-124.
https://doi.org/10.17660/ActaHortic.2005.695.13

Jones JB, Momol TM, Obradović A, Balogh B, Olson S. Bacterial spot management on tomatoes. in Proceedings of the 1st International Symposium on Tomato Diseases. 2005;(695):119-124.
doi:10.17660/ActaHortic.2005.695.13 .

Jones, Jeffrey B., Momol, Timur M., Obradović, Aleksa, Balogh, B, Olson, SM, "Bacterial spot management on tomatoes" in Proceedings of the 1st International Symposium on Tomato Diseases, no. 695 (2005):119-124,
https://doi.org/10.17660/ActaHortic.2005.695.13 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Olson, SM
AU  - Jackson, LE
AU  - Balogh, B
AU  - Guven, K
AU  - Iriarte, FB
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1017
AB  - Two strains of plant growth-promoting rhizobacteria, two systemic acquired resistance inducers (harpin and acibenzolar-S-methyl), host-specific unformulated bacteriophages, and two antagonistic bacteria were evaluated for control of tomato bacterial spot incited by Xanthomonas campestris pv. vesicatoria in greenhouse experiments. Untreated plants and plants treated with copper hydroxide were used as controls. The plant growth-promoting rhizobacteria or a tap water control were applied as a drench to the potting mix containing the seedlings, while the other treatments were applied to the foliage using a handheld sprayer. The plant growth-promoting rhizobacteria strains, when applied alone or in combination with other treatments, had no significant effect on bacterial spot intensity. Messenger and the antagonistic bacterial strains, when applied alone, had negligible effects on disease intensity. Unformulated phage or copper bactericide applications were inconsistent in performance under greenhouse conditions against bacterial spot. Although acibenzolar-S-methyl completely prevented occurrence of typical symptoms of the disease, necrotic spots typical of a hypersensitive reaction (HR) were observed on plants treated with acibenzolar-S-methyl alone. Electrolyte leakage and population dynamics experiments confirmed that acibenzolar-S-methyl-treated plants responded to inoculation by eliciting an HR. Application of bacteriophages in combination with acibenzolar-S-methyl suppressed a visible HR and provided excellent disease control. Although we were unable to quantify populations of the bacterium on the leaf surface, indirectly we determined that bacteriophages specific to the target bacterium reduced populations of a tomato race 3 strain of the pathogen on the leaf surface of acibenzolar-S-methyl-treated plants to levels that did not induce a visible HR. Integrated use of acibenzolar-S-m ethyl and phages may complement each other as an alternative management strategy against bacterial spot on tomato.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato
EP  - 716
IS  - 7
SP  - 712
VL  - 89
DO  - 10.1094/PD-89-0712
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Momol, Timur M. and Olson, SM and Jackson, LE and Balogh, B and Guven, K and Iriarte, FB",
year = "2005",
abstract = "Two strains of plant growth-promoting rhizobacteria, two systemic acquired resistance inducers (harpin and acibenzolar-S-methyl), host-specific unformulated bacteriophages, and two antagonistic bacteria were evaluated for control of tomato bacterial spot incited by Xanthomonas campestris pv. vesicatoria in greenhouse experiments. Untreated plants and plants treated with copper hydroxide were used as controls. The plant growth-promoting rhizobacteria or a tap water control were applied as a drench to the potting mix containing the seedlings, while the other treatments were applied to the foliage using a handheld sprayer. The plant growth-promoting rhizobacteria strains, when applied alone or in combination with other treatments, had no significant effect on bacterial spot intensity. Messenger and the antagonistic bacterial strains, when applied alone, had negligible effects on disease intensity. Unformulated phage or copper bactericide applications were inconsistent in performance under greenhouse conditions against bacterial spot. Although acibenzolar-S-methyl completely prevented occurrence of typical symptoms of the disease, necrotic spots typical of a hypersensitive reaction (HR) were observed on plants treated with acibenzolar-S-methyl alone. Electrolyte leakage and population dynamics experiments confirmed that acibenzolar-S-methyl-treated plants responded to inoculation by eliciting an HR. Application of bacteriophages in combination with acibenzolar-S-methyl suppressed a visible HR and provided excellent disease control. Although we were unable to quantify populations of the bacterium on the leaf surface, indirectly we determined that bacteriophages specific to the target bacterium reduced populations of a tomato race 3 strain of the pathogen on the leaf surface of acibenzolar-S-methyl-treated plants to levels that did not induce a visible HR. Integrated use of acibenzolar-S-m ethyl and phages may complement each other as an alternative management strategy against bacterial spot on tomato.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato",
pages = "716-712",
number = "7",
volume = "89",
doi = "10.1094/PD-89-0712"
}

Obradović, A., Jones, J. B., Momol, T. M., Olson, S., Jackson, L., Balogh, B., Guven, K.,& Iriarte, F.. (2005). Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. in Plant Disease
Amer Phytopathological Soc, St Paul., 89(7), 712-716.
https://doi.org/10.1094/PD-89-0712

Obradović A, Jones JB, Momol TM, Olson S, Jackson L, Balogh B, Guven K, Iriarte F. Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. in Plant Disease. 2005;89(7):712-716.
doi:10.1094/PD-89-0712 .

Obradović, Aleksa, Jones, Jeffrey B., Momol, Timur M., Olson, SM, Jackson, LE, Balogh, B, Guven, K, Iriarte, FB, "Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato" in Plant Disease, 89, no. 7 (2005):712-716,
https://doi.org/10.1094/PD-89-0712 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Balogh, B
AU  - Olson, SM
PY  - 2004
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/851
AB  - Various combinations of the harpin protein, acibenzolar-S-methyl, and bacteriophages were compared for controlling tomato bacterial spot in field experiments. Harpin protein and acibenzolar-S-methyl were applied every 14 days beginning twice before transplanting and then an additional four applications throughout the season. Formulated bacteriophages were applied prior to inoculation followed by twice a week at dusk. A standard bactericide treatment, consisting of copper hydroxide plus mancozeb, was applied once prior to inoculation and then every 7 days, while untreated plants served as an untreated control. Experiments were conducted in north and central Florida fields during fall 2001, spring 2002, and fall 2002. In three consecutive seasons, acibenzolar-S-methyl applied in combination with bacteriophage or bacteriophage and harpin significantly reduced bacterial spot compared with the other treatments. However, it did not significantly affect the total yield compared with the standard or untreated control. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all three experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared with the results of the corresponding nonbacteriophage group, the former provided significantly better disease control and yield of total marketable fruit.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers
EP  - 740
IS  - 7
SP  - 736
VL  - 88
DO  - 10.1094/PDIS.2004.88.7.736
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Momol, Timur M. and Balogh, B and Olson, SM",
year = "2004",
abstract = "Various combinations of the harpin protein, acibenzolar-S-methyl, and bacteriophages were compared for controlling tomato bacterial spot in field experiments. Harpin protein and acibenzolar-S-methyl were applied every 14 days beginning twice before transplanting and then an additional four applications throughout the season. Formulated bacteriophages were applied prior to inoculation followed by twice a week at dusk. A standard bactericide treatment, consisting of copper hydroxide plus mancozeb, was applied once prior to inoculation and then every 7 days, while untreated plants served as an untreated control. Experiments were conducted in north and central Florida fields during fall 2001, spring 2002, and fall 2002. In three consecutive seasons, acibenzolar-S-methyl applied in combination with bacteriophage or bacteriophage and harpin significantly reduced bacterial spot compared with the other treatments. However, it did not significantly affect the total yield compared with the standard or untreated control. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all three experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared with the results of the corresponding nonbacteriophage group, the former provided significantly better disease control and yield of total marketable fruit.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers",
pages = "740-736",
number = "7",
volume = "88",
doi = "10.1094/PDIS.2004.88.7.736"
}

Obradović, A., Jones, J. B., Momol, T. M., Balogh, B.,& Olson, S.. (2004). Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers. in Plant Disease
Amer Phytopathological Soc, St Paul., 88(7), 736-740.
https://doi.org/10.1094/PDIS.2004.88.7.736

Obradović A, Jones JB, Momol TM, Balogh B, Olson S. Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers. in Plant Disease. 2004;88(7):736-740.
doi:10.1094/PDIS.2004.88.7.736 .

Obradović, Aleksa, Jones, Jeffrey B., Momol, Timur M., Balogh, B, Olson, SM, "Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers" in Plant Disease, 88, no. 7 (2004):736-740,
https://doi.org/10.1094/PDIS.2004.88.7.736 . .

TY  - JOUR
AU  - Balogh, B
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Olson, SM
AU  - Obradović, Aleksa
AU  - King, P
AU  - Jackson, LE
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/641
AB  - Bacteriophages are currently used as an alternative method for controlling bacterial spot disease on tomato incited by Xanthomonas campestris pv. vesicatoria. However, the efficacy of phage is greatly reduced due to its short residual activity on plant foliage. Three formulations that significantly increased phage longevity on the plant surface were tested in field and greenhouse trials: (i) PCF, 0.5% pregelatinized corn flour (PCF) + 0.5% sucrose; (ii) Casecrete, 0.5% Casecrete NH-400 + 0.5% sucrose + 0.25% PCF; and (iii) skim milk, 0.75% powdered skim milk + 0.5% sucrose. In greenhouse experiments, the nonformulated, PCF-, Casecrete-, and skim milk-formulated phage mixtures reduced disease severity on plants compared with the control by 1, 30, 51, and 62%, respectively. In three consecutive field trials, nonformulated phage caused 15, 20, and 9% reduction in disease on treated plants compared with untreated control plants, whereas plants treated with PCF- and Casecrete-formulated phage had 27, 32, and 12% and 30, 43, and 24% disease reduction, respectively. Plants receiving copper-mancozeb treatments were included in two field trials and had a 20% decrease in disease in the first trial and a 13% increase in the second one. Skim milk-formulated phage was tested only once and caused an 18% disease reduction. PCF-formulated phage was more effective when applied in the evening than in the morning, reducing disease on plants by 27 and 13%, respectively. The Casecrete-formulated phage populations were over 1,000-fold higher than the nonformulated phage populations 36 h after phage application.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato
EP  - 954
IS  - 8
SP  - 949
VL  - 87
DO  - 10.1094/PDIS.2003.87.8.949
ER  - 

@article{
author = "Balogh, B and Jones, Jeffrey B. and Momol, Timur M. and Olson, SM and Obradović, Aleksa and King, P and Jackson, LE",
year = "2003",
abstract = "Bacteriophages are currently used as an alternative method for controlling bacterial spot disease on tomato incited by Xanthomonas campestris pv. vesicatoria. However, the efficacy of phage is greatly reduced due to its short residual activity on plant foliage. Three formulations that significantly increased phage longevity on the plant surface were tested in field and greenhouse trials: (i) PCF, 0.5% pregelatinized corn flour (PCF) + 0.5% sucrose; (ii) Casecrete, 0.5% Casecrete NH-400 + 0.5% sucrose + 0.25% PCF; and (iii) skim milk, 0.75% powdered skim milk + 0.5% sucrose. In greenhouse experiments, the nonformulated, PCF-, Casecrete-, and skim milk-formulated phage mixtures reduced disease severity on plants compared with the control by 1, 30, 51, and 62%, respectively. In three consecutive field trials, nonformulated phage caused 15, 20, and 9% reduction in disease on treated plants compared with untreated control plants, whereas plants treated with PCF- and Casecrete-formulated phage had 27, 32, and 12% and 30, 43, and 24% disease reduction, respectively. Plants receiving copper-mancozeb treatments were included in two field trials and had a 20% decrease in disease in the first trial and a 13% increase in the second one. Skim milk-formulated phage was tested only once and caused an 18% disease reduction. PCF-formulated phage was more effective when applied in the evening than in the morning, reducing disease on plants by 27 and 13%, respectively. The Casecrete-formulated phage populations were over 1,000-fold higher than the nonformulated phage populations 36 h after phage application.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato",
pages = "954-949",
number = "8",
volume = "87",
doi = "10.1094/PDIS.2003.87.8.949"
}

Balogh, B., Jones, J. B., Momol, T. M., Olson, S., Obradović, A., King, P.,& Jackson, L.. (2003). Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato. in Plant Disease
Amer Phytopathological Soc, St Paul., 87(8), 949-954.
https://doi.org/10.1094/PDIS.2003.87.8.949

Balogh B, Jones JB, Momol TM, Olson S, Obradović A, King P, Jackson L. Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato. in Plant Disease. 2003;87(8):949-954.
doi:10.1094/PDIS.2003.87.8.949 .

Balogh, B, Jones, Jeffrey B., Momol, Timur M., Olson, SM, Obradović, Aleksa, King, P, Jackson, LE, "Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato" in Plant Disease, 87, no. 8 (2003):949-954,
https://doi.org/10.1094/PDIS.2003.87.8.949 . .

TY  - CONF
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Minsavage, Gerald V.
AU  - Dickstein, Ellen R.
AU  - Momol, Timur M.
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/586
AB  - In October 2001, tomato foliage with blighting and a leaf spot was received from a transplant producer in Florida. The seedlings manifested apical or marginal leaf necrosis or discrete lesions along the leaf veins. Non-fluorescent bacterial strains forming viscous, creamy white colonies on King's medium B and causing a hypersensitive reaction in tomato and pepper leaves was consistently isolated from lesions. Pathogenicity was checked on three-week old tomato plants. The plants were incubated in high humidity for 24 h before and after inoculation. Similar symptoms were observed on the inoculated seedlings. We characterised 12 strains using bacteriological tests. According to the fatty acid profile, the strains displayed highest similarity with the bacterium Pseudomonas huttiensis Leifson 1962. Sequence analysis of the 16S rRNA indicated that this bacterium shows 98.7divided by98.8% homology with two Herbaspirillum species. Thus, our strains were compared with one P. huttiensis and five H. rubrisubalbicans strains. The strains from tomato were Gram-negative, non-fluorescent and oxidase positive, but levan, pectate hydrolysis and arginine dihydrolase negative, and grew at 40degreesC. Based on these results, they had a high degree of similarity with both P. huttiensis and H. rubrisubalbicans.
PB  - Springer, Dordrecht
C3  - Pseudomonas Syringae and Related Pathogens: Biology and Genetics
T1  - Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse
EP  - 630
SP  - 627
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_586
ER  - 

@conference{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Minsavage, Gerald V. and Dickstein, Ellen R. and Momol, Timur M.",
year = "2003",
abstract = "In October 2001, tomato foliage with blighting and a leaf spot was received from a transplant producer in Florida. The seedlings manifested apical or marginal leaf necrosis or discrete lesions along the leaf veins. Non-fluorescent bacterial strains forming viscous, creamy white colonies on King's medium B and causing a hypersensitive reaction in tomato and pepper leaves was consistently isolated from lesions. Pathogenicity was checked on three-week old tomato plants. The plants were incubated in high humidity for 24 h before and after inoculation. Similar symptoms were observed on the inoculated seedlings. We characterised 12 strains using bacteriological tests. According to the fatty acid profile, the strains displayed highest similarity with the bacterium Pseudomonas huttiensis Leifson 1962. Sequence analysis of the 16S rRNA indicated that this bacterium shows 98.7divided by98.8% homology with two Herbaspirillum species. Thus, our strains were compared with one P. huttiensis and five H. rubrisubalbicans strains. The strains from tomato were Gram-negative, non-fluorescent and oxidase positive, but levan, pectate hydrolysis and arginine dihydrolase negative, and grew at 40degreesC. Based on these results, they had a high degree of similarity with both P. huttiensis and H. rubrisubalbicans.",
publisher = "Springer, Dordrecht",
journal = "Pseudomonas Syringae and Related Pathogens: Biology and Genetics",
title = "Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse",
pages = "630-627",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_586"
}

Obradović, A., Jones, J. B., Minsavage, G. V., Dickstein, E. R.,& Momol, T. M.. (2003). Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics
Springer, Dordrecht., 627-630.
https://hdl.handle.net/21.15107/rcub_agrospace_586

Obradović A, Jones JB, Minsavage GV, Dickstein ER, Momol TM. Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse. in Pseudomonas Syringae and Related Pathogens: Biology and Genetics. 2003;:627-630.
https://hdl.handle.net/21.15107/rcub_agrospace_586 .

Obradović, Aleksa, Jones, Jeffrey B., Minsavage, Gerald V., Dickstein, Ellen R., Momol, Timur M., "Pseudomonas huttiensis associated with leaf necrosis and blighting of tomato seedlings in the greenhouse" in Pseudomonas Syringae and Related Pathogens: Biology and Genetics (2003):627-630,
https://hdl.handle.net/21.15107/rcub_agrospace_586 .