TY  - CONF
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Obradović, Aleksa
AU  - Balogh, B
AU  - Olson, SM
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1110
AB  - Bacterial spot of tomato, incited by Xanthomonas campestris pv. vesicatoria, is a perennial problem in commercial fields in Florida. Various strategies have been used to control this disease including applying copper bactericides, antibiotics and various other chemicals. Although copper compounds and streptomycin were initially effective in disease control, the presence of strains resistant to these compounds has reduced the efficacy of these compounds. Systemic acquired resistance (SAR) compounds have become a popular alternative to conventional bactericides. One that has shown promise for bacterial spot control is acibenzolar-S-methyl (ASM). Other SAR compounds tested have had variable results. A second strategy which has excellent promise is the application of bacteriophages specific to the bacterial spot pathogen. Following the development of an application strategy, which included a modification in formulation and the timing of bacteriophage applications, bacteriophages were improved in efficacy. We have conducted studies using various combinations of bacteriophages and SAR compounds. In three consecutive seasons, ASM applied in combination with bacteriophage significantly reduced bacterial spot compared to the other treatments. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared to the results of the corresponding non-bacteriophage group, the former provided significantly better disease control and yield of total marketable fruits. Although ASM applications controlled bacterial spot,yield was not significantly improved compared to plots receiving no ASM applications. Bacteriophages offer an excellent alternative to conventional disease control strategies and combining it with ASM shows promise.
PB  - International Society Horticultural Science, Leuven 1
C3  - Proceedings of the 1st International Symposium on Tomato Diseases
T1  - Bacterial spot management on tomatoes
EP  - 124
IS  - 695
SP  - 119
DO  - 10.17660/ActaHortic.2005.695.13
ER  - 

@conference{
author = "Jones, Jeffrey B. and Momol, Timur M. and Obradović, Aleksa and Balogh, B and Olson, SM",
year = "2005",
abstract = "Bacterial spot of tomato, incited by Xanthomonas campestris pv. vesicatoria, is a perennial problem in commercial fields in Florida. Various strategies have been used to control this disease including applying copper bactericides, antibiotics and various other chemicals. Although copper compounds and streptomycin were initially effective in disease control, the presence of strains resistant to these compounds has reduced the efficacy of these compounds. Systemic acquired resistance (SAR) compounds have become a popular alternative to conventional bactericides. One that has shown promise for bacterial spot control is acibenzolar-S-methyl (ASM). Other SAR compounds tested have had variable results. A second strategy which has excellent promise is the application of bacteriophages specific to the bacterial spot pathogen. Following the development of an application strategy, which included a modification in formulation and the timing of bacteriophage applications, bacteriophages were improved in efficacy. We have conducted studies using various combinations of bacteriophages and SAR compounds. In three consecutive seasons, ASM applied in combination with bacteriophage significantly reduced bacterial spot compared to the other treatments. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared to the results of the corresponding non-bacteriophage group, the former provided significantly better disease control and yield of total marketable fruits. Although ASM applications controlled bacterial spot,yield was not significantly improved compared to plots receiving no ASM applications. Bacteriophages offer an excellent alternative to conventional disease control strategies and combining it with ASM shows promise.",
publisher = "International Society Horticultural Science, Leuven 1",
journal = "Proceedings of the 1st International Symposium on Tomato Diseases",
title = "Bacterial spot management on tomatoes",
pages = "124-119",
number = "695",
doi = "10.17660/ActaHortic.2005.695.13"
}

Jones, J. B., Momol, T. M., Obradović, A., Balogh, B.,& Olson, S.. (2005). Bacterial spot management on tomatoes. in Proceedings of the 1st International Symposium on Tomato Diseases
International Society Horticultural Science, Leuven 1.(695), 119-124.
https://doi.org/10.17660/ActaHortic.2005.695.13

Jones JB, Momol TM, Obradović A, Balogh B, Olson S. Bacterial spot management on tomatoes. in Proceedings of the 1st International Symposium on Tomato Diseases. 2005;(695):119-124.
doi:10.17660/ActaHortic.2005.695.13 .

Jones, Jeffrey B., Momol, Timur M., Obradović, Aleksa, Balogh, B, Olson, SM, "Bacterial spot management on tomatoes" in Proceedings of the 1st International Symposium on Tomato Diseases, no. 695 (2005):119-124,
https://doi.org/10.17660/ActaHortic.2005.695.13 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Olson, SM
AU  - Jackson, LE
AU  - Balogh, B
AU  - Guven, K
AU  - Iriarte, FB
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1017
AB  - Two strains of plant growth-promoting rhizobacteria, two systemic acquired resistance inducers (harpin and acibenzolar-S-methyl), host-specific unformulated bacteriophages, and two antagonistic bacteria were evaluated for control of tomato bacterial spot incited by Xanthomonas campestris pv. vesicatoria in greenhouse experiments. Untreated plants and plants treated with copper hydroxide were used as controls. The plant growth-promoting rhizobacteria or a tap water control were applied as a drench to the potting mix containing the seedlings, while the other treatments were applied to the foliage using a handheld sprayer. The plant growth-promoting rhizobacteria strains, when applied alone or in combination with other treatments, had no significant effect on bacterial spot intensity. Messenger and the antagonistic bacterial strains, when applied alone, had negligible effects on disease intensity. Unformulated phage or copper bactericide applications were inconsistent in performance under greenhouse conditions against bacterial spot. Although acibenzolar-S-methyl completely prevented occurrence of typical symptoms of the disease, necrotic spots typical of a hypersensitive reaction (HR) were observed on plants treated with acibenzolar-S-methyl alone. Electrolyte leakage and population dynamics experiments confirmed that acibenzolar-S-methyl-treated plants responded to inoculation by eliciting an HR. Application of bacteriophages in combination with acibenzolar-S-methyl suppressed a visible HR and provided excellent disease control. Although we were unable to quantify populations of the bacterium on the leaf surface, indirectly we determined that bacteriophages specific to the target bacterium reduced populations of a tomato race 3 strain of the pathogen on the leaf surface of acibenzolar-S-methyl-treated plants to levels that did not induce a visible HR. Integrated use of acibenzolar-S-m ethyl and phages may complement each other as an alternative management strategy against bacterial spot on tomato.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato
EP  - 716
IS  - 7
SP  - 712
VL  - 89
DO  - 10.1094/PD-89-0712
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Momol, Timur M. and Olson, SM and Jackson, LE and Balogh, B and Guven, K and Iriarte, FB",
year = "2005",
abstract = "Two strains of plant growth-promoting rhizobacteria, two systemic acquired resistance inducers (harpin and acibenzolar-S-methyl), host-specific unformulated bacteriophages, and two antagonistic bacteria were evaluated for control of tomato bacterial spot incited by Xanthomonas campestris pv. vesicatoria in greenhouse experiments. Untreated plants and plants treated with copper hydroxide were used as controls. The plant growth-promoting rhizobacteria or a tap water control were applied as a drench to the potting mix containing the seedlings, while the other treatments were applied to the foliage using a handheld sprayer. The plant growth-promoting rhizobacteria strains, when applied alone or in combination with other treatments, had no significant effect on bacterial spot intensity. Messenger and the antagonistic bacterial strains, when applied alone, had negligible effects on disease intensity. Unformulated phage or copper bactericide applications were inconsistent in performance under greenhouse conditions against bacterial spot. Although acibenzolar-S-methyl completely prevented occurrence of typical symptoms of the disease, necrotic spots typical of a hypersensitive reaction (HR) were observed on plants treated with acibenzolar-S-methyl alone. Electrolyte leakage and population dynamics experiments confirmed that acibenzolar-S-methyl-treated plants responded to inoculation by eliciting an HR. Application of bacteriophages in combination with acibenzolar-S-methyl suppressed a visible HR and provided excellent disease control. Although we were unable to quantify populations of the bacterium on the leaf surface, indirectly we determined that bacteriophages specific to the target bacterium reduced populations of a tomato race 3 strain of the pathogen on the leaf surface of acibenzolar-S-methyl-treated plants to levels that did not induce a visible HR. Integrated use of acibenzolar-S-m ethyl and phages may complement each other as an alternative management strategy against bacterial spot on tomato.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato",
pages = "716-712",
number = "7",
volume = "89",
doi = "10.1094/PD-89-0712"
}

Obradović, A., Jones, J. B., Momol, T. M., Olson, S., Jackson, L., Balogh, B., Guven, K.,& Iriarte, F.. (2005). Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. in Plant Disease
Amer Phytopathological Soc, St Paul., 89(7), 712-716.
https://doi.org/10.1094/PD-89-0712

Obradović A, Jones JB, Momol TM, Olson S, Jackson L, Balogh B, Guven K, Iriarte F. Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. in Plant Disease. 2005;89(7):712-716.
doi:10.1094/PD-89-0712 .

Obradović, Aleksa, Jones, Jeffrey B., Momol, Timur M., Olson, SM, Jackson, LE, Balogh, B, Guven, K, Iriarte, FB, "Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato" in Plant Disease, 89, no. 7 (2005):712-716,
https://doi.org/10.1094/PD-89-0712 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Balogh, B
AU  - Olson, SM
PY  - 2004
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/851
AB  - Various combinations of the harpin protein, acibenzolar-S-methyl, and bacteriophages were compared for controlling tomato bacterial spot in field experiments. Harpin protein and acibenzolar-S-methyl were applied every 14 days beginning twice before transplanting and then an additional four applications throughout the season. Formulated bacteriophages were applied prior to inoculation followed by twice a week at dusk. A standard bactericide treatment, consisting of copper hydroxide plus mancozeb, was applied once prior to inoculation and then every 7 days, while untreated plants served as an untreated control. Experiments were conducted in north and central Florida fields during fall 2001, spring 2002, and fall 2002. In three consecutive seasons, acibenzolar-S-methyl applied in combination with bacteriophage or bacteriophage and harpin significantly reduced bacterial spot compared with the other treatments. However, it did not significantly affect the total yield compared with the standard or untreated control. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all three experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared with the results of the corresponding nonbacteriophage group, the former provided significantly better disease control and yield of total marketable fruit.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers
EP  - 740
IS  - 7
SP  - 736
VL  - 88
DO  - 10.1094/PDIS.2004.88.7.736
ER  - 

@article{
author = "Obradović, Aleksa and Jones, Jeffrey B. and Momol, Timur M. and Balogh, B and Olson, SM",
year = "2004",
abstract = "Various combinations of the harpin protein, acibenzolar-S-methyl, and bacteriophages were compared for controlling tomato bacterial spot in field experiments. Harpin protein and acibenzolar-S-methyl were applied every 14 days beginning twice before transplanting and then an additional four applications throughout the season. Formulated bacteriophages were applied prior to inoculation followed by twice a week at dusk. A standard bactericide treatment, consisting of copper hydroxide plus mancozeb, was applied once prior to inoculation and then every 7 days, while untreated plants served as an untreated control. Experiments were conducted in north and central Florida fields during fall 2001, spring 2002, and fall 2002. In three consecutive seasons, acibenzolar-S-methyl applied in combination with bacteriophage or bacteriophage and harpin significantly reduced bacterial spot compared with the other treatments. However, it did not significantly affect the total yield compared with the standard or untreated control. Application of host-specific bacteriophages was effective against the bacterial spot pathogen in all three experiments, providing better disease control than copper-mancozeb or untreated control. When results of the disease severity assessments or harvested yield from the bacteriophage-treated plots were grouped and compared with the results of the corresponding nonbacteriophage group, the former provided significantly better disease control and yield of total marketable fruit.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers",
pages = "740-736",
number = "7",
volume = "88",
doi = "10.1094/PDIS.2004.88.7.736"
}

Obradović, A., Jones, J. B., Momol, T. M., Balogh, B.,& Olson, S.. (2004). Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers. in Plant Disease
Amer Phytopathological Soc, St Paul., 88(7), 736-740.
https://doi.org/10.1094/PDIS.2004.88.7.736

Obradović A, Jones JB, Momol TM, Balogh B, Olson S. Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers. in Plant Disease. 2004;88(7):736-740.
doi:10.1094/PDIS.2004.88.7.736 .

Obradović, Aleksa, Jones, Jeffrey B., Momol, Timur M., Balogh, B, Olson, SM, "Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR inducers" in Plant Disease, 88, no. 7 (2004):736-740,
https://doi.org/10.1094/PDIS.2004.88.7.736 . .

TY  - JOUR
AU  - Balogh, B
AU  - Jones, Jeffrey B.
AU  - Momol, Timur M.
AU  - Olson, SM
AU  - Obradović, Aleksa
AU  - King, P
AU  - Jackson, LE
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/641
AB  - Bacteriophages are currently used as an alternative method for controlling bacterial spot disease on tomato incited by Xanthomonas campestris pv. vesicatoria. However, the efficacy of phage is greatly reduced due to its short residual activity on plant foliage. Three formulations that significantly increased phage longevity on the plant surface were tested in field and greenhouse trials: (i) PCF, 0.5% pregelatinized corn flour (PCF) + 0.5% sucrose; (ii) Casecrete, 0.5% Casecrete NH-400 + 0.5% sucrose + 0.25% PCF; and (iii) skim milk, 0.75% powdered skim milk + 0.5% sucrose. In greenhouse experiments, the nonformulated, PCF-, Casecrete-, and skim milk-formulated phage mixtures reduced disease severity on plants compared with the control by 1, 30, 51, and 62%, respectively. In three consecutive field trials, nonformulated phage caused 15, 20, and 9% reduction in disease on treated plants compared with untreated control plants, whereas plants treated with PCF- and Casecrete-formulated phage had 27, 32, and 12% and 30, 43, and 24% disease reduction, respectively. Plants receiving copper-mancozeb treatments were included in two field trials and had a 20% decrease in disease in the first trial and a 13% increase in the second one. Skim milk-formulated phage was tested only once and caused an 18% disease reduction. PCF-formulated phage was more effective when applied in the evening than in the morning, reducing disease on plants by 27 and 13%, respectively. The Casecrete-formulated phage populations were over 1,000-fold higher than the nonformulated phage populations 36 h after phage application.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato
EP  - 954
IS  - 8
SP  - 949
VL  - 87
DO  - 10.1094/PDIS.2003.87.8.949
ER  - 

@article{
author = "Balogh, B and Jones, Jeffrey B. and Momol, Timur M. and Olson, SM and Obradović, Aleksa and King, P and Jackson, LE",
year = "2003",
abstract = "Bacteriophages are currently used as an alternative method for controlling bacterial spot disease on tomato incited by Xanthomonas campestris pv. vesicatoria. However, the efficacy of phage is greatly reduced due to its short residual activity on plant foliage. Three formulations that significantly increased phage longevity on the plant surface were tested in field and greenhouse trials: (i) PCF, 0.5% pregelatinized corn flour (PCF) + 0.5% sucrose; (ii) Casecrete, 0.5% Casecrete NH-400 + 0.5% sucrose + 0.25% PCF; and (iii) skim milk, 0.75% powdered skim milk + 0.5% sucrose. In greenhouse experiments, the nonformulated, PCF-, Casecrete-, and skim milk-formulated phage mixtures reduced disease severity on plants compared with the control by 1, 30, 51, and 62%, respectively. In three consecutive field trials, nonformulated phage caused 15, 20, and 9% reduction in disease on treated plants compared with untreated control plants, whereas plants treated with PCF- and Casecrete-formulated phage had 27, 32, and 12% and 30, 43, and 24% disease reduction, respectively. Plants receiving copper-mancozeb treatments were included in two field trials and had a 20% decrease in disease in the first trial and a 13% increase in the second one. Skim milk-formulated phage was tested only once and caused an 18% disease reduction. PCF-formulated phage was more effective when applied in the evening than in the morning, reducing disease on plants by 27 and 13%, respectively. The Casecrete-formulated phage populations were over 1,000-fold higher than the nonformulated phage populations 36 h after phage application.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato",
pages = "954-949",
number = "8",
volume = "87",
doi = "10.1094/PDIS.2003.87.8.949"
}

Balogh, B., Jones, J. B., Momol, T. M., Olson, S., Obradović, A., King, P.,& Jackson, L.. (2003). Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato. in Plant Disease
Amer Phytopathological Soc, St Paul., 87(8), 949-954.
https://doi.org/10.1094/PDIS.2003.87.8.949

Balogh B, Jones JB, Momol TM, Olson S, Obradović A, King P, Jackson L. Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato. in Plant Disease. 2003;87(8):949-954.
doi:10.1094/PDIS.2003.87.8.949 .

Balogh, B, Jones, Jeffrey B., Momol, Timur M., Olson, SM, Obradović, Aleksa, King, P, Jackson, LE, "Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato" in Plant Disease, 87, no. 8 (2003):949-954,
https://doi.org/10.1094/PDIS.2003.87.8.949 . .