TY  - JOUR
AU  - Vukelić, Igor D.
AU  - Prokić, Ljiljana T.
AU  - Racić, Gordana M.
AU  - Pešić, Mirjana B.
AU  - Bojović, Mirjana M.
AU  - Sierka, Edyta M.
AU  - Kalaji, Hazem M.
AU  - Panković, Dejana M.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5883
AB  - The beneficial role of fungi from the Trichoderma genus and its secondary metabolites in promoting plant growth, uptake and use efficiency of macronutrients and oligo/micro‐nutrients, activation of plant secondary metabolism and plant protection from diseases makes it interesting for application in environmentally friendly agriculture. However, the literature data on the effect of Trichoderma inoculation on tomato fruit quality is scarce. Commercially used tomato cultivars were chosen in combination with indigenous Trichodrema species previously characterized on molecular and biochemical level, to investigate the effect of Trichoderma on photosynthetic characteristics and fruit quality of plants grown in organic system of production. Examined cultivars differed in the majority of examined parameters. Response of cultivar Gružanski zlatni to Tricho-derma application was more significant. As a consequence of increased epidermal flavonols and decreased chlorophyll, the nitrogen balance index in leaves has decreased, indicating a shift from primary to secondary metabolism. The quality of its fruit was altered in the sense of increased total flavonoids content, decreased starch, increased Bioaccumulation Index (BI) for Fe and Cr, and decreased BI for heavy metals Ni and Pb. Higher expression of swolenin gene in tomato roots of more responsive tomato cultivar indicates better root colonization, which correlates with observed positive effects of Trichodrema.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Effects of Trichoderma harzianum on Photosynthetic Characteristics and Fruit Quality of Tomato Plants
IS  - 13
SP  - 6961
VL  - 22
DO  - 10.3390/ijms22136961
ER  - 

@article{
author = "Vukelić, Igor D. and Prokić, Ljiljana T. and Racić, Gordana M. and Pešić, Mirjana B. and Bojović, Mirjana M. and Sierka, Edyta M. and Kalaji, Hazem M. and Panković, Dejana M.",
year = "2021",
abstract = "The beneficial role of fungi from the Trichoderma genus and its secondary metabolites in promoting plant growth, uptake and use efficiency of macronutrients and oligo/micro‐nutrients, activation of plant secondary metabolism and plant protection from diseases makes it interesting for application in environmentally friendly agriculture. However, the literature data on the effect of Trichoderma inoculation on tomato fruit quality is scarce. Commercially used tomato cultivars were chosen in combination with indigenous Trichodrema species previously characterized on molecular and biochemical level, to investigate the effect of Trichoderma on photosynthetic characteristics and fruit quality of plants grown in organic system of production. Examined cultivars differed in the majority of examined parameters. Response of cultivar Gružanski zlatni to Tricho-derma application was more significant. As a consequence of increased epidermal flavonols and decreased chlorophyll, the nitrogen balance index in leaves has decreased, indicating a shift from primary to secondary metabolism. The quality of its fruit was altered in the sense of increased total flavonoids content, decreased starch, increased Bioaccumulation Index (BI) for Fe and Cr, and decreased BI for heavy metals Ni and Pb. Higher expression of swolenin gene in tomato roots of more responsive tomato cultivar indicates better root colonization, which correlates with observed positive effects of Trichodrema.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Effects of Trichoderma harzianum on Photosynthetic Characteristics and Fruit Quality of Tomato Plants",
number = "13",
pages = "6961",
volume = "22",
doi = "10.3390/ijms22136961"
}

Vukelić, I. D., Prokić, L. T., Racić, G. M., Pešić, M. B., Bojović, M. M., Sierka, E. M., Kalaji, H. M.,& Panković, D. M.. (2021). Effects of Trichoderma harzianum on Photosynthetic Characteristics and Fruit Quality of Tomato Plants. in International Journal of Molecular Sciences
MDPI AG., 22(13), 6961.
https://doi.org/10.3390/ijms22136961

Vukelić ID, Prokić LT, Racić GM, Pešić MB, Bojović MM, Sierka EM, Kalaji HM, Panković DM. Effects of Trichoderma harzianum on Photosynthetic Characteristics and Fruit Quality of Tomato Plants. in International Journal of Molecular Sciences. 2021;22(13):6961.
doi:10.3390/ijms22136961 .

Vukelić, Igor D., Prokić, Ljiljana T., Racić, Gordana M., Pešić, Mirjana B., Bojović, Mirjana M., Sierka, Edyta M., Kalaji, Hazem M., Panković, Dejana M., "Effects of Trichoderma harzianum on Photosynthetic Characteristics and Fruit Quality of Tomato Plants" in International Journal of Molecular Sciences, 22, no. 13 (2021):6961,
https://doi.org/10.3390/ijms22136961 . .

TY  - CONF
AU  - Lalević, Blažo
AU  - Hamidović, Saud
AU  - Gavrić, Teofil
AU  - Sunulahpašić, A.
AU  - Borovac, B.
AU  - Halilović, M.
AU  - Jusić, I.
AU  - Kazlagić, A.
AU  - Delić, M.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5433
AB  - Modern agricultural production is often associated with application of agrochemicals. One of them is glyphosate, which has become one of the most widely used herbicides in weed control. However, the widespread use of glyphosate may influence the living organism’s activity. The aim of this paper was to estimate the soil microbial activity after glyphosate application. Glyphosate (Glifomark SG) was applied at the end of 2017 on two locations: Principovo polje, and Novi Travnik (Travnik municipality, Bosnia and Herzegovina), so that corn and raspberry residues can be removed. Soil sampling (0–10 and 10–30 cm) was conducted at the time of glyphosate application, also 15 and 45 days after application. On control soil glyphosate was not applied. Chemical soil analysis (pH, content of humus, available P and K) was performed using standard methodology, whilst microbial abundance (total number of bacteria, ammonification bacteria, fungi, actinomycetes and glyphosate-tolerant bacteria) was analyzed using agar plate method. The results of chemical soil analysis showed slightly acid to slightly alkaline pH value, moderate to high humus content, and low to high content of available P and K. In most of samples, bacterial number was reduced 15 days after glyphosate application, whilst at the end of experiment increase of bacterial abundance was recorded. Similar results were obtained for fungi. Rapid decrease of actinomycetes number was observed after application of glyphosate. Two bacterial strains (PP-23 and NT-11) were able to grow on mineral medium supplemented with glyphosate in concentrations of 1 and 2% (v/v), thus representing a promising candidates for bioremediation of soil contaminated with glyphosate.
PB  - Springer
C3  - IFMBE Proceedings
T1  - Survival of soil microbial population after glyphosate application
EP  - 43
SP  - 36
VL  - 78
DO  - 10.1007/978-3-030-40049-1_4
ER  - 

@conference{
author = "Lalević, Blažo and Hamidović, Saud and Gavrić, Teofil and Sunulahpašić, A. and Borovac, B. and Halilović, M. and Jusić, I. and Kazlagić, A. and Delić, M.",
year = "2020",
abstract = "Modern agricultural production is often associated with application of agrochemicals. One of them is glyphosate, which has become one of the most widely used herbicides in weed control. However, the widespread use of glyphosate may influence the living organism’s activity. The aim of this paper was to estimate the soil microbial activity after glyphosate application. Glyphosate (Glifomark SG) was applied at the end of 2017 on two locations: Principovo polje, and Novi Travnik (Travnik municipality, Bosnia and Herzegovina), so that corn and raspberry residues can be removed. Soil sampling (0–10 and 10–30 cm) was conducted at the time of glyphosate application, also 15 and 45 days after application. On control soil glyphosate was not applied. Chemical soil analysis (pH, content of humus, available P and K) was performed using standard methodology, whilst microbial abundance (total number of bacteria, ammonification bacteria, fungi, actinomycetes and glyphosate-tolerant bacteria) was analyzed using agar plate method. The results of chemical soil analysis showed slightly acid to slightly alkaline pH value, moderate to high humus content, and low to high content of available P and K. In most of samples, bacterial number was reduced 15 days after glyphosate application, whilst at the end of experiment increase of bacterial abundance was recorded. Similar results were obtained for fungi. Rapid decrease of actinomycetes number was observed after application of glyphosate. Two bacterial strains (PP-23 and NT-11) were able to grow on mineral medium supplemented with glyphosate in concentrations of 1 and 2% (v/v), thus representing a promising candidates for bioremediation of soil contaminated with glyphosate.",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Survival of soil microbial population after glyphosate application",
pages = "43-36",
volume = "78",
doi = "10.1007/978-3-030-40049-1_4"
}

Lalević, B., Hamidović, S., Gavrić, T., Sunulahpašić, A., Borovac, B., Halilović, M., Jusić, I., Kazlagić, A.,& Delić, M.. (2020). Survival of soil microbial population after glyphosate application. in IFMBE Proceedings
Springer., 78, 36-43.
https://doi.org/10.1007/978-3-030-40049-1_4

Lalević B, Hamidović S, Gavrić T, Sunulahpašić A, Borovac B, Halilović M, Jusić I, Kazlagić A, Delić M. Survival of soil microbial population after glyphosate application. in IFMBE Proceedings. 2020;78:36-43.
doi:10.1007/978-3-030-40049-1_4 .

Lalević, Blažo, Hamidović, Saud, Gavrić, Teofil, Sunulahpašić, A., Borovac, B., Halilović, M., Jusić, I., Kazlagić, A., Delić, M., "Survival of soil microbial population after glyphosate application" in IFMBE Proceedings, 78 (2020):36-43,
https://doi.org/10.1007/978-3-030-40049-1_4 . .

TY  - JOUR
AU  - Ilić, Dora S.
AU  - Dimkić, Ivica
AU  - Waisi, Hadi K.
AU  - Gkorezis, Panagiotis M.
AU  - Hamidović, Saud
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4922
AB  - Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil
EP  - 258
IS  - 3
SP  - 247
VL  - 25
DO  - 10.2298/CICEQ180607003I
ER  - 

@article{
author = "Ilić, Dora S. and Dimkić, Ivica and Waisi, Hadi K. and Gkorezis, Panagiotis M. and Hamidović, Saud and Raičević, Vera and Lalević, Blažo",
year = "2019",
abstract = "Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil",
pages = "258-247",
number = "3",
volume = "25",
doi = "10.2298/CICEQ180607003I"
}

Ilić, D. S., Dimkić, I., Waisi, H. K., Gkorezis, P. M., Hamidović, S., Raičević, V.,& Lalević, B.. (2019). Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 25(3), 247-258.
https://doi.org/10.2298/CICEQ180607003I

Ilić DS, Dimkić I, Waisi HK, Gkorezis PM, Hamidović S, Raičević V, Lalević B. Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2019;25(3):247-258.
doi:10.2298/CICEQ180607003I .

Ilić, Dora S., Dimkić, Ivica, Waisi, Hadi K., Gkorezis, Panagiotis M., Hamidović, Saud, Raičević, Vera, Lalević, Blažo, "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 25, no. 3 (2019):247-258,
https://doi.org/10.2298/CICEQ180607003I . .

TY  - JOUR
AU  - Milinković, Mira
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
AU  - Golubović-Ćurguz, Vesna
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5075
AB  - Besides ecological and environmental benefits of green open spaces, horticultural waste management has various environmental consequences. Green waste composting represents promising environmentally friendly alternative which gives valuable products with positive soil and plants impact. Composting products' quality determinates their application and depends on the particular waste material and process parameters. The aim of this paper was to estimate the chemical and microbiological quality of green waste compost and compost products (compost tea, compost extract and the solid phase after extraction), and their biopotential based on germination rate, germination index and inhibition of phytopathogenic fungi growth. Higher germination rate of examined plant seeds was noticed on the solid phase after extraction, and compost extract, compared to compost, and compost tea, respectively. Plants with low germination rate grown on compost showed higher fresh and dry biomass. Compost products strongly inhibited the growth of plant pathogens Fusarium oxysporum, Rhizoctonia sp., and Pythium debaryanum. Presented results show that composted biodegradable waste from urban green spaces contribute to the plant growth and phytopathogenic fungi suppression, and thus improve the overall environmental quality.
PB  - Inst Chemical Engineers, Rugby
T2  - Process Safety and Environmental Protection
T1  - Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression
EP  - 306
SP  - 299
VL  - 121
DO  - 10.1016/j.psep.2018.09.024
ER  - 

@article{
author = "Milinković, Mira and Lalević, Blažo and Jovičić-Petrović, Jelena and Golubović-Ćurguz, Vesna and Kljujev, Igor and Raičević, Vera",
year = "2019",
abstract = "Besides ecological and environmental benefits of green open spaces, horticultural waste management has various environmental consequences. Green waste composting represents promising environmentally friendly alternative which gives valuable products with positive soil and plants impact. Composting products' quality determinates their application and depends on the particular waste material and process parameters. The aim of this paper was to estimate the chemical and microbiological quality of green waste compost and compost products (compost tea, compost extract and the solid phase after extraction), and their biopotential based on germination rate, germination index and inhibition of phytopathogenic fungi growth. Higher germination rate of examined plant seeds was noticed on the solid phase after extraction, and compost extract, compared to compost, and compost tea, respectively. Plants with low germination rate grown on compost showed higher fresh and dry biomass. Compost products strongly inhibited the growth of plant pathogens Fusarium oxysporum, Rhizoctonia sp., and Pythium debaryanum. Presented results show that composted biodegradable waste from urban green spaces contribute to the plant growth and phytopathogenic fungi suppression, and thus improve the overall environmental quality.",
publisher = "Inst Chemical Engineers, Rugby",
journal = "Process Safety and Environmental Protection",
title = "Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression",
pages = "306-299",
volume = "121",
doi = "10.1016/j.psep.2018.09.024"
}

Milinković, M., Lalević, B., Jovičić-Petrović, J., Golubović-Ćurguz, V., Kljujev, I.,& Raičević, V.. (2019). Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression. in Process Safety and Environmental Protection
Inst Chemical Engineers, Rugby., 121, 299-306.
https://doi.org/10.1016/j.psep.2018.09.024

Milinković M, Lalević B, Jovičić-Petrović J, Golubović-Ćurguz V, Kljujev I, Raičević V. Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression. in Process Safety and Environmental Protection. 2019;121:299-306.
doi:10.1016/j.psep.2018.09.024 .

Milinković, Mira, Lalević, Blažo, Jovičić-Petrović, Jelena, Golubović-Ćurguz, Vesna, Kljujev, Igor, Raičević, Vera, "Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression" in Process Safety and Environmental Protection, 121 (2019):299-306,
https://doi.org/10.1016/j.psep.2018.09.024 . .

TY  - JOUR
AU  - Sunulahpašić, Amer
AU  - Hamidović, Saud
AU  - Mitrić, Siniša
AU  - Gavrić, Teofil
AU  - Haseljić, Sanel
AU  - Lalević, Blažo
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5223
AB  - Nicosulfuron belongs to the sulfonylurea pesticides, which are widely used for weeds control. Except of benefits in plant production, long-term application of nicosulfuron may have toxic effect for living organisms, including microorganisms. The aim of this paper was to determined impact of nicosulfuron on microbial diversity of soil. Sampling of soil (0-20 and 20-40 cm) treated with nicosulfuron at village Trenica (Novi Travnik municipality, Bosnia and Herzegovina) was performed in autumn 2017. Determination of microbial diversity (total number of bacteria, ammonification bacteria, fungi and actinomycetes) was performed using standard methodology, whilst nicosulfuron-tolerant bacteria were isolated using enrichment method. Soil without nicosulfuron application was used as a control. The results showed that bacteria were most abundant microbial population. In all experiments, reduction of microbial diversity in nicosulfuron-treated soil compared to untreated was observed. This reduction was most expressed in fungal number, which is reduced from 38 to 60% compared to control. Several nicosulfuron-tolerant isolates were isolated by enrichment method. By microscopic observation and using API test kits and APIWEB database, isolates 17cs, and 22wl and 5 wl were identified as Pseudomonas fluorescens, and Bacillus subtilis, respectively. These bacterial isolates could be applied in remediation of environments polluted by nicosulfuron.
AB  - Nikosulfuron pripada grupi pesticide sulfonil urea, koji imaju široku primenu u uništavanju korova. Iako je njegova primena korisna sa aspekta uspešne biljne proizvodnje, njegova višegodišnja upotreba može imati toksične efekte za živi svet, uključujući i mikroorganizme. Cilj ovog rada bio je ispitivanje uticaja nikosulfurona na mikrobni diverzitet zemljišta. Uzorkovanje zemljišta (0-20 i 20-40 cm) tretiranog nikosulfuronom na području sela Trenica (Opština Novi Travnik, Bosna i Hercegovina) obavljeno je u jesen 2017. godine. Određivanje mikrobnog diverziteta (ukupnog broja bakterija, amonifikatora, gljiva I aktinomiceta) izvršeno je korišćenjem standardnih metoda, dok su bakterije tolerantne na prisustvo nikosulfurona određene metodom obogaćenja. Kontrolu je predstavljalo zemljište koje nije tretirano nikosulfuronom. Rezultati ukazuju da su bakterije bile najbrojnija populacija mikroorganizama. U svim varijantama ogleda konstatovana je redukcija mikrobnog diverziteta u zemljištu koje je tretirano nikosulfuronom u odnosu na kontrolni uzorak. Stepen redukcije bio je najveći kod gljiva, čija je brojnost redukovana za 38-60% u odnosu na kontrolu. Nekoliko izolata bakterija tolerantnih na prisustvo nikosulfurona je izolovano iz uzoraka zemljišta metodom obogaćenja. Mikroskopskim ispitivanjem I pomoću API i APIWEB metode, izolati 17cs, odnosno 22wl i 5wl, su identifikovani kao Pseudomonas fluorescens, odnosno Bacillus subtilis. Ovi bakterijski izolati bi mogli da imaju potencijalnu primenu u remedijaciji ekosistema kontaminiranih nikosulfuronom.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Assessment of microbial diversity of soil exposed to nicosulfuron
T1  - Ispitivanje mikrobnog diverziteta zemljišta tretiranog nikosulfuronom
EP  - 156
IS  - 2
SP  - 152
VL  - 60
DO  - 10.5937/zasmat1902152S
ER  - 

@article{
author = "Sunulahpašić, Amer and Hamidović, Saud and Mitrić, Siniša and Gavrić, Teofil and Haseljić, Sanel and Lalević, Blažo",
year = "2019",
abstract = "Nicosulfuron belongs to the sulfonylurea pesticides, which are widely used for weeds control. Except of benefits in plant production, long-term application of nicosulfuron may have toxic effect for living organisms, including microorganisms. The aim of this paper was to determined impact of nicosulfuron on microbial diversity of soil. Sampling of soil (0-20 and 20-40 cm) treated with nicosulfuron at village Trenica (Novi Travnik municipality, Bosnia and Herzegovina) was performed in autumn 2017. Determination of microbial diversity (total number of bacteria, ammonification bacteria, fungi and actinomycetes) was performed using standard methodology, whilst nicosulfuron-tolerant bacteria were isolated using enrichment method. Soil without nicosulfuron application was used as a control. The results showed that bacteria were most abundant microbial population. In all experiments, reduction of microbial diversity in nicosulfuron-treated soil compared to untreated was observed. This reduction was most expressed in fungal number, which is reduced from 38 to 60% compared to control. Several nicosulfuron-tolerant isolates were isolated by enrichment method. By microscopic observation and using API test kits and APIWEB database, isolates 17cs, and 22wl and 5 wl were identified as Pseudomonas fluorescens, and Bacillus subtilis, respectively. These bacterial isolates could be applied in remediation of environments polluted by nicosulfuron., Nikosulfuron pripada grupi pesticide sulfonil urea, koji imaju široku primenu u uništavanju korova. Iako je njegova primena korisna sa aspekta uspešne biljne proizvodnje, njegova višegodišnja upotreba može imati toksične efekte za živi svet, uključujući i mikroorganizme. Cilj ovog rada bio je ispitivanje uticaja nikosulfurona na mikrobni diverzitet zemljišta. Uzorkovanje zemljišta (0-20 i 20-40 cm) tretiranog nikosulfuronom na području sela Trenica (Opština Novi Travnik, Bosna i Hercegovina) obavljeno je u jesen 2017. godine. Određivanje mikrobnog diverziteta (ukupnog broja bakterija, amonifikatora, gljiva I aktinomiceta) izvršeno je korišćenjem standardnih metoda, dok su bakterije tolerantne na prisustvo nikosulfurona određene metodom obogaćenja. Kontrolu je predstavljalo zemljište koje nije tretirano nikosulfuronom. Rezultati ukazuju da su bakterije bile najbrojnija populacija mikroorganizama. U svim varijantama ogleda konstatovana je redukcija mikrobnog diverziteta u zemljištu koje je tretirano nikosulfuronom u odnosu na kontrolni uzorak. Stepen redukcije bio je najveći kod gljiva, čija je brojnost redukovana za 38-60% u odnosu na kontrolu. Nekoliko izolata bakterija tolerantnih na prisustvo nikosulfurona je izolovano iz uzoraka zemljišta metodom obogaćenja. Mikroskopskim ispitivanjem I pomoću API i APIWEB metode, izolati 17cs, odnosno 22wl i 5wl, su identifikovani kao Pseudomonas fluorescens, odnosno Bacillus subtilis. Ovi bakterijski izolati bi mogli da imaju potencijalnu primenu u remedijaciji ekosistema kontaminiranih nikosulfuronom.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Assessment of microbial diversity of soil exposed to nicosulfuron, Ispitivanje mikrobnog diverziteta zemljišta tretiranog nikosulfuronom",
pages = "156-152",
number = "2",
volume = "60",
doi = "10.5937/zasmat1902152S"
}

Sunulahpašić, A., Hamidović, S., Mitrić, S., Gavrić, T., Haseljić, S.,& Lalević, B.. (2019). Assessment of microbial diversity of soil exposed to nicosulfuron. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 60(2), 152-156.
https://doi.org/10.5937/zasmat1902152S

Sunulahpašić A, Hamidović S, Mitrić S, Gavrić T, Haseljić S, Lalević B. Assessment of microbial diversity of soil exposed to nicosulfuron. in Zaštita materijala. 2019;60(2):152-156.
doi:10.5937/zasmat1902152S .

Sunulahpašić, Amer, Hamidović, Saud, Mitrić, Siniša, Gavrić, Teofil, Haseljić, Sanel, Lalević, Blažo, "Assessment of microbial diversity of soil exposed to nicosulfuron" in Zaštita materijala, 60, no. 2 (2019):152-156,
https://doi.org/10.5937/zasmat1902152S . .

TY  - JOUR
AU  - Milinković, M.
AU  - Lalević, Blažo
AU  - Raičević, Vera
AU  - Paunović, S.M.
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4697
AB  - Fruits of five apple cultivars were treated using 1-methylcyclopropene (1-MCP or SmartFresh (TM)) after cropping and were stored at normal atmosphere 2 +/- 0.5 degrees C, 90 +/- 5% relative humidity (RH) and 20.9 kPa O-2 +  lt 0.5 kPa CO2. Fruit firmness was assessed at three periods: 7 d after storing, 120 d after storing and 30 d after the second assessment and storing at room temperature. Contents of K in all of the cultivars and in all years of study varied within the average values between 1390.5 and 2028.0 mg kg(-1), while the Ca content varied between 21.7 and 59.5 mg kg(-1). The K:Ca ratio was the lowest in cultivar 'Granny Smith' (24.0) and the highest in 'Redchief ' (99.1). Application of 1-MCP made the strongest impact on fruit firmness of the cultivars 'Granny Smith' and 'Idared' in all measuring periods. Cultivars 'Redchier', 'Cadel' and 'Morrens Ionagored' responded well to the application of 1-MCP in the storage conditions, whereas the effect of its application influenced conservability of the fruits stored at room temperature except in fruits of the cultivar 'Morens Jonagored'. Application of 1-MCP made an important effect on the preservation of fruit firmness, all in accordance with the degree of ripeness of the fruits subjected to the treatment and the contents of K, Ca and K:Ca ratio. This study indicates that the use of 1-MCP treatment in post harvest handling of apples is promising for maintaining the freshness and quality of fruits.
PB  - Druckerei Liddy Halm, Gottingen
T2  - Journal of Applied Botany and Food Quality
T1  - Application of 1-methylcyclopropene in fruit of five apple cultivars grown in Serbia
EP  - 303
SP  - 296
VL  - 91
DO  - 10.5073/JABFQ.2018.091.038
ER  - 

@article{
author = "Milinković, M. and Lalević, Blažo and Raičević, Vera and Paunović, S.M.",
year = "2018",
abstract = "Fruits of five apple cultivars were treated using 1-methylcyclopropene (1-MCP or SmartFresh (TM)) after cropping and were stored at normal atmosphere 2 +/- 0.5 degrees C, 90 +/- 5% relative humidity (RH) and 20.9 kPa O-2 +  lt 0.5 kPa CO2. Fruit firmness was assessed at three periods: 7 d after storing, 120 d after storing and 30 d after the second assessment and storing at room temperature. Contents of K in all of the cultivars and in all years of study varied within the average values between 1390.5 and 2028.0 mg kg(-1), while the Ca content varied between 21.7 and 59.5 mg kg(-1). The K:Ca ratio was the lowest in cultivar 'Granny Smith' (24.0) and the highest in 'Redchief ' (99.1). Application of 1-MCP made the strongest impact on fruit firmness of the cultivars 'Granny Smith' and 'Idared' in all measuring periods. Cultivars 'Redchier', 'Cadel' and 'Morrens Ionagored' responded well to the application of 1-MCP in the storage conditions, whereas the effect of its application influenced conservability of the fruits stored at room temperature except in fruits of the cultivar 'Morens Jonagored'. Application of 1-MCP made an important effect on the preservation of fruit firmness, all in accordance with the degree of ripeness of the fruits subjected to the treatment and the contents of K, Ca and K:Ca ratio. This study indicates that the use of 1-MCP treatment in post harvest handling of apples is promising for maintaining the freshness and quality of fruits.",
publisher = "Druckerei Liddy Halm, Gottingen",
journal = "Journal of Applied Botany and Food Quality",
title = "Application of 1-methylcyclopropene in fruit of five apple cultivars grown in Serbia",
pages = "303-296",
volume = "91",
doi = "10.5073/JABFQ.2018.091.038"
}

Milinković, M., Lalević, B., Raičević, V.,& Paunović, S.M.. (2018). Application of 1-methylcyclopropene in fruit of five apple cultivars grown in Serbia. in Journal of Applied Botany and Food Quality
Druckerei Liddy Halm, Gottingen., 91, 296-303.
https://doi.org/10.5073/JABFQ.2018.091.038

Milinković M, Lalević B, Raičević V, Paunović S. Application of 1-methylcyclopropene in fruit of five apple cultivars grown in Serbia. in Journal of Applied Botany and Food Quality. 2018;91:296-303.
doi:10.5073/JABFQ.2018.091.038 .

Milinković, M., Lalević, Blažo, Raičević, Vera, Paunović, S.M., "Application of 1-methylcyclopropene in fruit of five apple cultivars grown in Serbia" in Journal of Applied Botany and Food Quality, 91 (2018):296-303,
https://doi.org/10.5073/JABFQ.2018.091.038 . .

TY  - CONF
AU  - Vujović, Bojana
AU  - Jovičić-Petrović, Jelena
AU  - Rašković, Nikola
AU  - Vukmanović, Tamara
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6844
AB  - Podzemne vode predstavljaju najočuvaniji deo hidrosfere, zaštićen od globalne
degradacije kvaliteta vode, zbog čega je široko rasprostranjen i često korišćen resurs
vodosnabdevanja. Iako se podzemne vode smatraju mikrobiološki slabo naseljenim
delom biosfere, transformacija organskog ugljenika, transformacija azota, gvožđa i
sumpora između oksidovanog i redukovanog stanja, produkcija metana i drugi
fundamentalni procesi se i u podzemnim vodama odigravaju mikrobiološkom
aktivnošću.1 Iako bakterije koje pripadaju različitim fiziološkim grupama nisu od
primarnog značaja pri proceni pogodnosti vode za upotrebu, populacija "normalno" ili
"prirodno" prisutnih bakterija utiče na organoleptička svojstva, pH, redoks potencijal,
količinu kiseonika i druge karakteristike vode.1,2 U radu su prikazani rezultati
kvalitativne i kvantitativne zastupljenosti gvožđevitih, sulfat redukujućih, nitrifikujućih,
denitrifikujućih, fluorescirajućih i sluz produkujućih bakterija u vodi poreklom iz tri
bunara primenom BART testova (Biological Activity Reaction Tests). Takođe, u radu je
prikazan i broj ukupnih i fekalnih koliformnih bakterija određen metodom IDEXX
Colilert-18/Quanty-Tray 2000 i fekalnih enterokoka metodom Enterolert-E/Quanty-Tray
2000. Dobijeni rezultati ukazuju da postoji pozitivna korelacija između biodiverziteta
različitih grupa testiranih bakterija, što ukazuje na složene ekološke abiotičke i biotičke
odnose u podzemenim vodama i bunarima. Iako je autohtona bakterijska zajednica
uslovljena geološkim svojstvima vode, ove bakterije mogu uticati i limitirati upotrebu
podzemne vode u vodosnabdevanju stupanjem u odnose sa patogenim ili potencijalno
patogenim organizmima
C3  - knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo
T1  - Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6844
ER  - 

@conference{
author = "Vujović, Bojana and Jovičić-Petrović, Jelena and Rašković, Nikola and Vukmanović, Tamara and Kljujev, Igor and Raičević, Vera",
year = "2018",
abstract = "Podzemne vode predstavljaju najočuvaniji deo hidrosfere, zaštićen od globalne
degradacije kvaliteta vode, zbog čega je široko rasprostranjen i često korišćen resurs
vodosnabdevanja. Iako se podzemne vode smatraju mikrobiološki slabo naseljenim
delom biosfere, transformacija organskog ugljenika, transformacija azota, gvožđa i
sumpora između oksidovanog i redukovanog stanja, produkcija metana i drugi
fundamentalni procesi se i u podzemnim vodama odigravaju mikrobiološkom
aktivnošću.1 Iako bakterije koje pripadaju različitim fiziološkim grupama nisu od
primarnog značaja pri proceni pogodnosti vode za upotrebu, populacija "normalno" ili
"prirodno" prisutnih bakterija utiče na organoleptička svojstva, pH, redoks potencijal,
količinu kiseonika i druge karakteristike vode.1,2 U radu su prikazani rezultati
kvalitativne i kvantitativne zastupljenosti gvožđevitih, sulfat redukujućih, nitrifikujućih,
denitrifikujućih, fluorescirajućih i sluz produkujućih bakterija u vodi poreklom iz tri
bunara primenom BART testova (Biological Activity Reaction Tests). Takođe, u radu je
prikazan i broj ukupnih i fekalnih koliformnih bakterija određen metodom IDEXX
Colilert-18/Quanty-Tray 2000 i fekalnih enterokoka metodom Enterolert-E/Quanty-Tray
2000. Dobijeni rezultati ukazuju da postoji pozitivna korelacija između biodiverziteta
različitih grupa testiranih bakterija, što ukazuje na složene ekološke abiotičke i biotičke
odnose u podzemenim vodama i bunarima. Iako je autohtona bakterijska zajednica
uslovljena geološkim svojstvima vode, ove bakterije mogu uticati i limitirati upotrebu
podzemne vode u vodosnabdevanju stupanjem u odnose sa patogenim ili potencijalno
patogenim organizmima",
journal = "knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo",
title = "Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6844"
}

Vujović, B., Jovičić-Petrović, J., Rašković, N., Vukmanović, T., Kljujev, I.,& Raičević, V.. (2018). Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi. in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo.
https://hdl.handle.net/21.15107/rcub_agrospace_6844

Vujović B, Jovičić-Petrović J, Rašković N, Vukmanović T, Kljujev I, Raičević V. Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi. in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo. 2018;.
https://hdl.handle.net/21.15107/rcub_agrospace_6844 .

Vujović, Bojana, Jovičić-Petrović, Jelena, Rašković, Nikola, Vukmanović, Tamara, Kljujev, Igor, Raičević, Vera, "Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi" in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo (2018),
https://hdl.handle.net/21.15107/rcub_agrospace_6844 .

TY  - JOUR
AU  - Rudić, Željka
AU  - Vujović, Bojana
AU  - Božić, Mile
AU  - Arizanović, Tanja
AU  - Raičević, Vera
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4621
AB  - The objective of this paper was to emphasise how faecal bacteria persist in canal sediment and relate abiotic factors in a pen-urban canal. Mann-Whitney tests confirmed that there is a considerable difference in water quality of upstream and downstream ends of the canal, which supports the claim that water quality is affected by wastewater discharge. Strong positive correlations between enterococci and organic matter, TOC and all nitrogen forms in sediment show that the large amount of organic matter in sediment is linked to high enterococcal counts. High counts of E. coli in sediment, as well as weak correlations between E. coli and sediment quality parameters, together with water quality changes, indicate a 'fresh' pollution of aquatic system. In conclusion, our study suggests that wastewater discharge makes a long-term contribution to the increased content of E. cob and enterococci in the Palic-Ludas canal.
PB  - Inderscience Enterprises Ltd, Geneva
T2  - International Journal of Environment and Pollution
T1  - Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia
EP  - 309
IS  - 4
SP  - 292
VL  - 64
DO  - 10.1504/IJEP.2018.099463
ER  - 

@article{
author = "Rudić, Željka and Vujović, Bojana and Božić, Mile and Arizanović, Tanja and Raičević, Vera",
year = "2018",
abstract = "The objective of this paper was to emphasise how faecal bacteria persist in canal sediment and relate abiotic factors in a pen-urban canal. Mann-Whitney tests confirmed that there is a considerable difference in water quality of upstream and downstream ends of the canal, which supports the claim that water quality is affected by wastewater discharge. Strong positive correlations between enterococci and organic matter, TOC and all nitrogen forms in sediment show that the large amount of organic matter in sediment is linked to high enterococcal counts. High counts of E. coli in sediment, as well as weak correlations between E. coli and sediment quality parameters, together with water quality changes, indicate a 'fresh' pollution of aquatic system. In conclusion, our study suggests that wastewater discharge makes a long-term contribution to the increased content of E. cob and enterococci in the Palic-Ludas canal.",
publisher = "Inderscience Enterprises Ltd, Geneva",
journal = "International Journal of Environment and Pollution",
title = "Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia",
pages = "309-292",
number = "4",
volume = "64",
doi = "10.1504/IJEP.2018.099463"
}

Rudić, Ž., Vujović, B., Božić, M., Arizanović, T.,& Raičević, V.. (2018). Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia. in International Journal of Environment and Pollution
Inderscience Enterprises Ltd, Geneva., 64(4), 292-309.
https://doi.org/10.1504/IJEP.2018.099463

Rudić Ž, Vujović B, Božić M, Arizanović T, Raičević V. Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia. in International Journal of Environment and Pollution. 2018;64(4):292-309.
doi:10.1504/IJEP.2018.099463 .

Rudić, Željka, Vujović, Bojana, Božić, Mile, Arizanović, Tanja, Raičević, Vera, "Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia" in International Journal of Environment and Pollution, 64, no. 4 (2018):292-309,
https://doi.org/10.1504/IJEP.2018.099463 . .

TY  - JOUR
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Vujović, Bojana
AU  - Mirković, Milica
AU  - Rothballer, Michael
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4692
AB  - The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Listeria monocytogenes - Danger for health safety vegetable production
EP  - 31
SP  - 23
VL  - 120
DO  - 10.1016/j.micpath.2018.04.034
ER  - 

@article{
author = "Kljujev, Igor and Raičević, Vera and Jovičić-Petrović, Jelena and Vujović, Bojana and Mirković, Milica and Rothballer, Michael",
year = "2018",
abstract = "The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Listeria monocytogenes - Danger for health safety vegetable production",
pages = "31-23",
volume = "120",
doi = "10.1016/j.micpath.2018.04.034"
}

Kljujev, I., Raičević, V., Jovičić-Petrović, J., Vujović, B., Mirković, M.,& Rothballer, M.. (2018). Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 120, 23-31.
https://doi.org/10.1016/j.micpath.2018.04.034

Kljujev I, Raičević V, Jovičić-Petrović J, Vujović B, Mirković M, Rothballer M. Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis. 2018;120:23-31.
doi:10.1016/j.micpath.2018.04.034 .

Kljujev, Igor, Raičević, Vera, Jovičić-Petrović, Jelena, Vujović, Bojana, Mirković, Milica, Rothballer, Michael, "Listeria monocytogenes - Danger for health safety vegetable production" in Microbial Pathogenesis, 120 (2018):23-31,
https://doi.org/10.1016/j.micpath.2018.04.034 . .

TY  - JOUR
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Vujović, Bojana
AU  - Rothballer, Michael
AU  - Schmid, Michael
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4720
AB  - Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production
EP  - 207
SP  - 199
VL  - 115
DO  - 10.1016/j.micpath.2017.12.020
ER  - 

@article{
author = "Kljujev, Igor and Raičević, Vera and Vujović, Bojana and Rothballer, Michael and Schmid, Michael",
year = "2018",
abstract = "Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production",
pages = "207-199",
volume = "115",
doi = "10.1016/j.micpath.2017.12.020"
}

Kljujev, I., Raičević, V., Vujović, B., Rothballer, M.,& Schmid, M.. (2018). Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 115, 199-207.
https://doi.org/10.1016/j.micpath.2017.12.020

Kljujev I, Raičević V, Vujović B, Rothballer M, Schmid M. Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis. 2018;115:199-207.
doi:10.1016/j.micpath.2017.12.020 .

Kljujev, Igor, Raičević, Vera, Vujović, Bojana, Rothballer, Michael, Schmid, Michael, "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production" in Microbial Pathogenesis, 115 (2018):199-207,
https://doi.org/10.1016/j.micpath.2017.12.020 . .

TY  - JOUR
AU  - Racić, Gordana
AU  - Vukelić, Igor
AU  - Prokić, Ljiljana
AU  - Curcić, Nataša
AU  - Zorić, Miroslav
AU  - Jovanović, Ljubinko
AU  - Panković, Dejana
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4668
AB  - Trichoderma species are widely known for their antagonistic properties, but plant growth promotion and abiotic stress tolerance have also been reported. Mechanisms of abiotic stress tolerance, such as drought in plants in interaction with Trichoderma spp., remain unclear. In this work, the results about the influence of Trichoderma brevicompactum on the physiological parameters and abscisic acid (ABA) content in leaves and roots of tomato, as well as the Trichodermamodulated plant response to drought are presented. Both in optimally watered plants and droughted plants treated with Trichoderma, the relative water content (RWC) was 17% higher than in untreated plants. Drought lowered RWC by 25% in both Trichoderma-untreated and -treated plants. Under drought conditions, the better water status of plants treated with Trichoderma is connected with lower stomatal conductance and closure of stomata. The lower increase of root ABA content in Trichoderma-treated plants that we observed could be an adaptive advantage in sense of smaller inhibition of plant growth induced by drought. Trichoderma modulation of the salicylic acid (SA) marker genes response to drought was observed in leaves. Our results suggest that signalling pathways of jasmonic acid (JA), SA and ABA are involved in response to both drought and Trichoderma. The mutual effect of the tested treatments leads to the modification of gene expression in comparison to the reaction to a single stress. The upregulation of SA marker genes in leaves and JA marker genes in the roots 2 weeks after the interaction with T. brevicompactum indicates that it could be promising biocontrol agent.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato
EP  - 221
IS  - 3
SP  - 213
VL  - 173
DO  - 10.1111/aab.12454
ER  - 

@article{
author = "Racić, Gordana and Vukelić, Igor and Prokić, Ljiljana and Curcić, Nataša and Zorić, Miroslav and Jovanović, Ljubinko and Panković, Dejana",
year = "2018",
abstract = "Trichoderma species are widely known for their antagonistic properties, but plant growth promotion and abiotic stress tolerance have also been reported. Mechanisms of abiotic stress tolerance, such as drought in plants in interaction with Trichoderma spp., remain unclear. In this work, the results about the influence of Trichoderma brevicompactum on the physiological parameters and abscisic acid (ABA) content in leaves and roots of tomato, as well as the Trichodermamodulated plant response to drought are presented. Both in optimally watered plants and droughted plants treated with Trichoderma, the relative water content (RWC) was 17% higher than in untreated plants. Drought lowered RWC by 25% in both Trichoderma-untreated and -treated plants. Under drought conditions, the better water status of plants treated with Trichoderma is connected with lower stomatal conductance and closure of stomata. The lower increase of root ABA content in Trichoderma-treated plants that we observed could be an adaptive advantage in sense of smaller inhibition of plant growth induced by drought. Trichoderma modulation of the salicylic acid (SA) marker genes response to drought was observed in leaves. Our results suggest that signalling pathways of jasmonic acid (JA), SA and ABA are involved in response to both drought and Trichoderma. The mutual effect of the tested treatments leads to the modification of gene expression in comparison to the reaction to a single stress. The upregulation of SA marker genes in leaves and JA marker genes in the roots 2 weeks after the interaction with T. brevicompactum indicates that it could be promising biocontrol agent.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato",
pages = "221-213",
number = "3",
volume = "173",
doi = "10.1111/aab.12454"
}

Racić, G., Vukelić, I., Prokić, L., Curcić, N., Zorić, M., Jovanović, L.,& Panković, D.. (2018). The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato. in Annals of Applied Biology
Wiley, Hoboken., 173(3), 213-221.
https://doi.org/10.1111/aab.12454

Racić G, Vukelić I, Prokić L, Curcić N, Zorić M, Jovanović L, Panković D. The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato. in Annals of Applied Biology. 2018;173(3):213-221.
doi:10.1111/aab.12454 .

Racić, Gordana, Vukelić, Igor, Prokić, Ljiljana, Curcić, Nataša, Zorić, Miroslav, Jovanović, Ljubinko, Panković, Dejana, "The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato" in Annals of Applied Biology, 173, no. 3 (2018):213-221,
https://doi.org/10.1111/aab.12454 . .

TY  - JOUR
AU  - Racić, Gordana
AU  - Kormoczi, Peter
AU  - Kredics, Laszlo
AU  - Raičević, Vera
AU  - Mutavdzić, Beba
AU  - Vrvić, Miroslav M.
AU  - Panković, Dejana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4384
AB  - Influence of edaphic factors and metal content on diversity of Trichoderma species at 14 different soil sampling locations, on two depths, was examined. Forty-one Trichoderma isolates from 14 sampling sites were determined as nine species based on their internal transcribed spacer (ITS) sequences. Our results indicate that weakly alkaline soils are rich sources of Trichoderma strains. Also, higher contents of available K and P are connected with higher Trichoderma diversity. Increased metal content in soil was not inhibiting factor for Trichoderma species occurrence. Relationship between these factors was confirmed by locally weighted sequential smoothing (LOESS) nonparametric smoothing analysis. Trichoderma strain (Szeged Microbiology Collection (SZMC) 22669) from soil with concentrations of Cr and Ni above remediation values should be tested for its potential for bioremediation of these metals in polluted soils.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.
EP  - 3386
IS  - 4
SP  - 3375
VL  - 24
DO  - 10.1007/s11356-016-8067-1
ER  - 

@article{
author = "Racić, Gordana and Kormoczi, Peter and Kredics, Laszlo and Raičević, Vera and Mutavdzić, Beba and Vrvić, Miroslav M. and Panković, Dejana",
year = "2017",
abstract = "Influence of edaphic factors and metal content on diversity of Trichoderma species at 14 different soil sampling locations, on two depths, was examined. Forty-one Trichoderma isolates from 14 sampling sites were determined as nine species based on their internal transcribed spacer (ITS) sequences. Our results indicate that weakly alkaline soils are rich sources of Trichoderma strains. Also, higher contents of available K and P are connected with higher Trichoderma diversity. Increased metal content in soil was not inhibiting factor for Trichoderma species occurrence. Relationship between these factors was confirmed by locally weighted sequential smoothing (LOESS) nonparametric smoothing analysis. Trichoderma strain (Szeged Microbiology Collection (SZMC) 22669) from soil with concentrations of Cr and Ni above remediation values should be tested for its potential for bioremediation of these metals in polluted soils.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.",
pages = "3386-3375",
number = "4",
volume = "24",
doi = "10.1007/s11356-016-8067-1"
}

Racić, G., Kormoczi, P., Kredics, L., Raičević, V., Mutavdzić, B., Vrvić, M. M.,& Panković, D.. (2017). Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(4), 3375-3386.
https://doi.org/10.1007/s11356-016-8067-1

Racić G, Kormoczi P, Kredics L, Raičević V, Mutavdzić B, Vrvić MM, Panković D. Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.. in Environmental Science and Pollution Research. 2017;24(4):3375-3386.
doi:10.1007/s11356-016-8067-1 .

Racić, Gordana, Kormoczi, Peter, Kredics, Laszlo, Raičević, Vera, Mutavdzić, Beba, Vrvić, Miroslav M., Panković, Dejana, "Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp." in Environmental Science and Pollution Research, 24, no. 4 (2017):3375-3386,
https://doi.org/10.1007/s11356-016-8067-1 . .

TY  - JOUR
AU  - Waisi, Hadi K.
AU  - Petković, Andjelka Z.
AU  - Nikolić, Bogdan R.
AU  - Janković, Bojan Z.
AU  - Raičević, Vera
AU  - Lalević, Blažo
AU  - Giba, Zlatko S.
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4461
AB  - In this study, influence of wide range of 24-epibrassinolide (24-EBL) on early growth potential of two maize hybrids (ZP 434 and ZP 704) was examined. Paper concerns germination, seedling biomass, important chlorophylls content, and redistribution of elements (heavy metals and microelements), in a seedlings of the maize hybrids, as influenced by different 24-EBL concentrations. It was found that hybrids react differently to exogenously applied hormone. The biggest differences between two examined maize hybrids considering the germination level were reached with the lowest values at 86% for ZP 704 and 72% for ZP 434, gained at the highest applied concentration of 24-EBL. Seedlings of hybrid ZP 434 reacted positively moderately in the case of shoot length and biomass under the influence of 24-EBL, but seedlings of hybrid ZP 704 had lower values of these parameters under the influence of the phytohormone. Chlorophyll a/b ratios showed that photosynthetic apparatus of seedlings of the hybrids is not active in this stage of development. It was established that 24-EBL affects seedling growth and re-allocation of naturally present mineral elements in early growth stages and that could be one of the reason for poorer growth of ZP 704 treated with various concentrations of 24-EBL, comparing to control. When applied in lower concentrations, 24-EBL is blocking toxic elements such as chromium and nickel to relocate to vital parts of plant, what was case in hybrid ZP704. In case of ZP 434, lower concentrations of 24-EBL are affecting re-allocation of Cu and Cr and these findings suggest that maize hybrid seedlings treated with lower concentrations of 24-EBL could survive and be successful in polluted areas.
PB  - Savez hemijskih inženjera, Beograd
T2  - HEMIJSKA INDUSTRIJA
T1  - Influence of 24-epibrassinolide on seedling growth and distribution of mineral elements in two maize hybrids
EP  - 209
IS  - 3
SP  - 201
VL  - 71
DO  - 10.2298/HEMIND160318030W
ER  - 

@article{
author = "Waisi, Hadi K. and Petković, Andjelka Z. and Nikolić, Bogdan R. and Janković, Bojan Z. and Raičević, Vera and Lalević, Blažo and Giba, Zlatko S.",
year = "2017",
abstract = "In this study, influence of wide range of 24-epibrassinolide (24-EBL) on early growth potential of two maize hybrids (ZP 434 and ZP 704) was examined. Paper concerns germination, seedling biomass, important chlorophylls content, and redistribution of elements (heavy metals and microelements), in a seedlings of the maize hybrids, as influenced by different 24-EBL concentrations. It was found that hybrids react differently to exogenously applied hormone. The biggest differences between two examined maize hybrids considering the germination level were reached with the lowest values at 86% for ZP 704 and 72% for ZP 434, gained at the highest applied concentration of 24-EBL. Seedlings of hybrid ZP 434 reacted positively moderately in the case of shoot length and biomass under the influence of 24-EBL, but seedlings of hybrid ZP 704 had lower values of these parameters under the influence of the phytohormone. Chlorophyll a/b ratios showed that photosynthetic apparatus of seedlings of the hybrids is not active in this stage of development. It was established that 24-EBL affects seedling growth and re-allocation of naturally present mineral elements in early growth stages and that could be one of the reason for poorer growth of ZP 704 treated with various concentrations of 24-EBL, comparing to control. When applied in lower concentrations, 24-EBL is blocking toxic elements such as chromium and nickel to relocate to vital parts of plant, what was case in hybrid ZP704. In case of ZP 434, lower concentrations of 24-EBL are affecting re-allocation of Cu and Cr and these findings suggest that maize hybrid seedlings treated with lower concentrations of 24-EBL could survive and be successful in polluted areas.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "HEMIJSKA INDUSTRIJA",
title = "Influence of 24-epibrassinolide on seedling growth and distribution of mineral elements in two maize hybrids",
pages = "209-201",
number = "3",
volume = "71",
doi = "10.2298/HEMIND160318030W"
}

Waisi, H. K., Petković, A. Z., Nikolić, B. R., Janković, B. Z., Raičević, V., Lalević, B.,& Giba, Z. S.. (2017). Influence of 24-epibrassinolide on seedling growth and distribution of mineral elements in two maize hybrids. in HEMIJSKA INDUSTRIJA
Savez hemijskih inženjera, Beograd., 71(3), 201-209.
https://doi.org/10.2298/HEMIND160318030W

Waisi HK, Petković AZ, Nikolić BR, Janković BZ, Raičević V, Lalević B, Giba ZS. Influence of 24-epibrassinolide on seedling growth and distribution of mineral elements in two maize hybrids. in HEMIJSKA INDUSTRIJA. 2017;71(3):201-209.
doi:10.2298/HEMIND160318030W .

Waisi, Hadi K., Petković, Andjelka Z., Nikolić, Bogdan R., Janković, Bojan Z., Raičević, Vera, Lalević, Blažo, Giba, Zlatko S., "Influence of 24-epibrassinolide on seedling growth and distribution of mineral elements in two maize hybrids" in HEMIJSKA INDUSTRIJA, 71, no. 3 (2017):201-209,
https://doi.org/10.2298/HEMIND160318030W . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Morina, Filis
AU  - Golubović-Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4466
AB  - Overburden waste dumps represent a huge threat to environmental quality. The reduction of their negative impact can be achieved by vegetation cover establishment. Usually, this action is complicated due to site-specific characteristics, such as nutrient deficiency, elevated metal concentration, low pH value, lack of moisture and lack of organic matter. Establishment of vegetation can be facilitated by inoculation with plant growth promoting bacteria (PGPB) which improve the physicochemical and biological properties of degraded substrates and make them more hospitable for plants. In this study we selected several strains based on the ability to produce ammonia, indole-3-acetic acid, siderophores and lytic enzymes, and to solubilize inorganic phosphates. This selection resulted in microbial consortia consisting of Serratia liquefaciens Z-I ARV, Ensifer adhaerens 10_ARV, Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. The effects of PGPB consortia on one-year-old London plane (Platanus x acerifolia [Aiton] Willd.) seedlings replanted into overburden waste from Kolubara Mine Basin were examined. After seven months, inoculated seedlings were 32% higher with 45% wider root collar diameter and over 80% higher total dry biomass compared to uninoculated seedlings grown in Kolubara's overburden. Inoculation resulted in higher amounts of total soluble proteins, higher chlorophyll and epidermal flavonoids content and higher total antioxidative capacity in the leaves. This study represents a successful search for effective PGPB strains and shows that microbial consortia have an important role in enhancing the growth of seedlings in nutrient deficient and degraded substrates such as overburden waste from open-pit coal mines. Positive response of London plane seedlings suggest that inoculation may help widening the opus of species for reforestation of post mining areas and speed up natural succession processes and recovery of degraded landscapes.
PB  - Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza
T2  - Iforest-Biogeosciences and Forestry
T1  - Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia
EP  - 699
SP  - 692
VL  - 10
DO  - 10.3832/ifor2135-010
ER  - 

@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Lalević, Blažo and Morina, Filis and Golubović-Ćurguz, Vesna and Raičević, Vera",
year = "2017",
abstract = "Overburden waste dumps represent a huge threat to environmental quality. The reduction of their negative impact can be achieved by vegetation cover establishment. Usually, this action is complicated due to site-specific characteristics, such as nutrient deficiency, elevated metal concentration, low pH value, lack of moisture and lack of organic matter. Establishment of vegetation can be facilitated by inoculation with plant growth promoting bacteria (PGPB) which improve the physicochemical and biological properties of degraded substrates and make them more hospitable for plants. In this study we selected several strains based on the ability to produce ammonia, indole-3-acetic acid, siderophores and lytic enzymes, and to solubilize inorganic phosphates. This selection resulted in microbial consortia consisting of Serratia liquefaciens Z-I ARV, Ensifer adhaerens 10_ARV, Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. The effects of PGPB consortia on one-year-old London plane (Platanus x acerifolia [Aiton] Willd.) seedlings replanted into overburden waste from Kolubara Mine Basin were examined. After seven months, inoculated seedlings were 32% higher with 45% wider root collar diameter and over 80% higher total dry biomass compared to uninoculated seedlings grown in Kolubara's overburden. Inoculation resulted in higher amounts of total soluble proteins, higher chlorophyll and epidermal flavonoids content and higher total antioxidative capacity in the leaves. This study represents a successful search for effective PGPB strains and shows that microbial consortia have an important role in enhancing the growth of seedlings in nutrient deficient and degraded substrates such as overburden waste from open-pit coal mines. Positive response of London plane seedlings suggest that inoculation may help widening the opus of species for reforestation of post mining areas and speed up natural succession processes and recovery of degraded landscapes.",
publisher = "Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza",
journal = "Iforest-Biogeosciences and Forestry",
title = "Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia",
pages = "699-692",
volume = "10",
doi = "10.3832/ifor2135-010"
}

Karličić, V., Radić, D., Jovičić-Petrović, J., Lalević, B., Morina, F., Golubović-Ćurguz, V.,& Raičević, V.. (2017). Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia. in Iforest-Biogeosciences and Forestry
Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza., 10, 692-699.
https://doi.org/10.3832/ifor2135-010

Karličić V, Radić D, Jovičić-Petrović J, Lalević B, Morina F, Golubović-Ćurguz V, Raičević V. Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia. in Iforest-Biogeosciences and Forestry. 2017;10:692-699.
doi:10.3832/ifor2135-010 .

Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Lalević, Blažo, Morina, Filis, Golubović-Ćurguz, Vesna, Raičević, Vera, "Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia" in Iforest-Biogeosciences and Forestry, 10 (2017):692-699,
https://doi.org/10.3832/ifor2135-010 . .

TY  - JOUR
AU  - Radić, Danka
AU  - Pavlović, Vera P.
AU  - Lazović, Milana
AU  - Jovičić-Petrović, Jelena
AU  - Karličić, Vera
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4484
AB  - Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism
EP  - 21893
IS  - 27
SP  - 21885
VL  - 24
DO  - 10.1007/s11356-017-9817-4
ER  - 

@article{
author = "Radić, Danka and Pavlović, Vera P. and Lazović, Milana and Jovičić-Petrović, Jelena and Karličić, Vera and Lalević, Blažo and Raičević, Vera",
year = "2017",
abstract = "Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism",
pages = "21893-21885",
number = "27",
volume = "24",
doi = "10.1007/s11356-017-9817-4"
}

Radić, D., Pavlović, V. P., Lazović, M., Jovičić-Petrović, J., Karličić, V., Lalević, B.,& Raičević, V.. (2017). Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(27), 21885-21893.
https://doi.org/10.1007/s11356-017-9817-4

Radić D, Pavlović VP, Lazović M, Jovičić-Petrović J, Karličić V, Lalević B, Raičević V. Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research. 2017;24(27):21885-21893.
doi:10.1007/s11356-017-9817-4 .

Radić, Danka, Pavlović, Vera P., Lazović, Milana, Jovičić-Petrović, Jelena, Karličić, Vera, Lalević, Blažo, Raičević, Vera, "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism" in Environmental Science and Pollution Research, 24, no. 27 (2017):21885-21893,
https://doi.org/10.1007/s11356-017-9817-4 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Živanović, Iva
AU  - Matijašević, Danka
AU  - Raičević, Vera
AU  - Nikšić, Miomir
AU  - Rac, Vladislav
AU  - Simić, Aleksandar
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4607
AB  - Zeolites can mitigate ammonium losses to the environment by taking up the ions from sources such as farmyard manure and ammonium-bearing fertilizers. This ability can improve nitrogen fertilizing practice given that ammonium ions loaded onto zeolite are available for plant uptake. The main goal of this study was to assess the influence of zeolite (clinoptilolite) and ammonia-loaded clinoptilolite on growth and yield of red clover (Trifolium pratense L.), as well as their influence on the microbial dynamics in soil. Plants sown in pots were cut four times, and dry matter yield (DM) of each harvest was weighed. The number of culturable bacteria, moulds, ammonifiers, Azotobacter sp. and actinomycetes was determined at the beginning of the experiment, and after each harvest. Two control treatments (soil without fertilizer and soil supplemented with mineral fertilizer - CAN) were included in the study. The application of ammonia-loaded clinoptilolite positively and significantly affected the microbial activity and provided higher yields (from 4.9 g/pot in ammonia-loaded clinoptilolite treatment to 4.3 g/pot in soil with zeolite treatment). Our results suggest that the addition of clinoptilolite to organic fertilizers (manure, composts) could be recommended. The increase of microbial communities’ abundances and their activity represented the key benefit from clinoptilolite application.
AB  - Zeoliti mogu ublažiti otpuštanje amonijaka u prirodu preko uzimanja jona iz izvora kao što su stajnjak i đubriva bogata amonijakom. Ova sposobnost može unaprediti đubrenje azotom preko zeolitom usvojenih amonijum jona koji bi tako postali dostupni za usvajanje biljkama. Cilj ovih ispitivanja je bio procena uticaja zeolita (klinoptilolita) i zeolita obogaćenog amonijakom na rast i prinos crvene deteline (Trifolium pratense L.), kao i na njihovu ulogu u mikrobiološkoj dinamici zemljišta. Biljke sejane u sudovima su košene četiri puta i meren je prinos suve materije (SM) svakog otkosa. Broj bakterija, plesni, amonifikatora, Azotobacter sp. i aktinomiceta je utvrđen na početku ogleda i nakon svakog košenja. U istraživanja su uključena dva kontrolna tretmana (zemljište bez đubriva i zemljište sa dodatim mineralnim đubrivom - KAN). Primena klinoptilolita obogaćenog sa amonijakom je pozitivno i značajno uticala na mikrobiološku aktivnost i povećala je prinos (od 4,9 g po sudu kod tretmana sa klinoptilolitom obogaćenim amonijakom do 4,3 g po sudu u zemljištu sa zemljištem i zeolitom zajedno). Naša istraživanja sugerišu da se može preporučiti dodavanje klinoptilolita organskim đubrivima (stajnjaku, kompostu). Ključna dobit od primene klinoptilolita bi se ogledala u povećanju zastupljenosti mikrobioloških zajednica i njihove aktivnosti.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth
T1  - Stimulacija zemljišne mikrobiološke aktivnosti sa klinoptilolitom - uticaj na rast biljke
EP  - 123
IS  - 3
SP  - 117
VL  - 54
DO  - 10.5937/ratpov54-14870
ER  - 

@article{
author = "Karličić, Vera and Živanović, Iva and Matijašević, Danka and Raičević, Vera and Nikšić, Miomir and Rac, Vladislav and Simić, Aleksandar",
year = "2017",
abstract = "Zeolites can mitigate ammonium losses to the environment by taking up the ions from sources such as farmyard manure and ammonium-bearing fertilizers. This ability can improve nitrogen fertilizing practice given that ammonium ions loaded onto zeolite are available for plant uptake. The main goal of this study was to assess the influence of zeolite (clinoptilolite) and ammonia-loaded clinoptilolite on growth and yield of red clover (Trifolium pratense L.), as well as their influence on the microbial dynamics in soil. Plants sown in pots were cut four times, and dry matter yield (DM) of each harvest was weighed. The number of culturable bacteria, moulds, ammonifiers, Azotobacter sp. and actinomycetes was determined at the beginning of the experiment, and after each harvest. Two control treatments (soil without fertilizer and soil supplemented with mineral fertilizer - CAN) were included in the study. The application of ammonia-loaded clinoptilolite positively and significantly affected the microbial activity and provided higher yields (from 4.9 g/pot in ammonia-loaded clinoptilolite treatment to 4.3 g/pot in soil with zeolite treatment). Our results suggest that the addition of clinoptilolite to organic fertilizers (manure, composts) could be recommended. The increase of microbial communities’ abundances and their activity represented the key benefit from clinoptilolite application., Zeoliti mogu ublažiti otpuštanje amonijaka u prirodu preko uzimanja jona iz izvora kao što su stajnjak i đubriva bogata amonijakom. Ova sposobnost može unaprediti đubrenje azotom preko zeolitom usvojenih amonijum jona koji bi tako postali dostupni za usvajanje biljkama. Cilj ovih ispitivanja je bio procena uticaja zeolita (klinoptilolita) i zeolita obogaćenog amonijakom na rast i prinos crvene deteline (Trifolium pratense L.), kao i na njihovu ulogu u mikrobiološkoj dinamici zemljišta. Biljke sejane u sudovima su košene četiri puta i meren je prinos suve materije (SM) svakog otkosa. Broj bakterija, plesni, amonifikatora, Azotobacter sp. i aktinomiceta je utvrđen na početku ogleda i nakon svakog košenja. U istraživanja su uključena dva kontrolna tretmana (zemljište bez đubriva i zemljište sa dodatim mineralnim đubrivom - KAN). Primena klinoptilolita obogaćenog sa amonijakom je pozitivno i značajno uticala na mikrobiološku aktivnost i povećala je prinos (od 4,9 g po sudu kod tretmana sa klinoptilolitom obogaćenim amonijakom do 4,3 g po sudu u zemljištu sa zemljištem i zeolitom zajedno). Naša istraživanja sugerišu da se može preporučiti dodavanje klinoptilolita organskim đubrivima (stajnjaku, kompostu). Ključna dobit od primene klinoptilolita bi se ogledala u povećanju zastupljenosti mikrobioloških zajednica i njihove aktivnosti.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth, Stimulacija zemljišne mikrobiološke aktivnosti sa klinoptilolitom - uticaj na rast biljke",
pages = "123-117",
number = "3",
volume = "54",
doi = "10.5937/ratpov54-14870"
}

Karličić, V., Živanović, I., Matijašević, D., Raičević, V., Nikšić, M., Rac, V.,& Simić, A.. (2017). Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(3), 117-123.
https://doi.org/10.5937/ratpov54-14870

Karličić V, Živanović I, Matijašević D, Raičević V, Nikšić M, Rac V, Simić A. Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth. in Ratarstvo i povrtarstvo. 2017;54(3):117-123.
doi:10.5937/ratpov54-14870 .

Karličić, Vera, Živanović, Iva, Matijašević, Danka, Raičević, Vera, Nikšić, Miomir, Rac, Vladislav, Simić, Aleksandar, "Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth" in Ratarstvo i povrtarstvo, 54, no. 3 (2017):117-123,
https://doi.org/10.5937/ratpov54-14870 . .

TY  - JOUR
AU  - Waisi, Hadi K.
AU  - Janković, Bojan
AU  - Janković, Marija
AU  - Nikolić, Bogdan
AU  - Dimkić, Ivica
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4344
AB  - Proposed distributed reactivity model of dehydration for seedling parts of two various maize hybrids (ZP434, ZP704) was established. Dehydration stresses were induced thermally, which is also accompanied by response of hybrids to heat stress. It was found that an increased value of activation energy counterparts within radicle dehydration of ZP434, with a high concentration of 24-epibrassinolide (24-EBL) at elevated operating temperatures, probably causes activation of diffusion mechanisms in cutin network and may increases likelihood of formation of free volumes, large enough to accommodate diffusing molecule. Many small random effects were detected and can be correlated with micro-disturbing in a space filled with water caused by thermal gradients, increasing capillary phenomena, and which can induce thermo-capillary migration. The influence of seedling content of various sugars and minerals on dehydration was also examined. Estimated distributed reactivity models indicate a dependence of reactivity on structural arrangements, due to present interactions between water molecules and chemical species within the plant.
PB  - Public Library Science, San Francisco
T2  - Plos One
T1  - New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide
IS  - 6
VL  - 12
DO  - 10.1371/journal.pone.0179650
ER  - 

@article{
author = "Waisi, Hadi K. and Janković, Bojan and Janković, Marija and Nikolić, Bogdan and Dimkić, Ivica and Lalević, Blažo and Raičević, Vera",
year = "2017",
abstract = "Proposed distributed reactivity model of dehydration for seedling parts of two various maize hybrids (ZP434, ZP704) was established. Dehydration stresses were induced thermally, which is also accompanied by response of hybrids to heat stress. It was found that an increased value of activation energy counterparts within radicle dehydration of ZP434, with a high concentration of 24-epibrassinolide (24-EBL) at elevated operating temperatures, probably causes activation of diffusion mechanisms in cutin network and may increases likelihood of formation of free volumes, large enough to accommodate diffusing molecule. Many small random effects were detected and can be correlated with micro-disturbing in a space filled with water caused by thermal gradients, increasing capillary phenomena, and which can induce thermo-capillary migration. The influence of seedling content of various sugars and minerals on dehydration was also examined. Estimated distributed reactivity models indicate a dependence of reactivity on structural arrangements, due to present interactions between water molecules and chemical species within the plant.",
publisher = "Public Library Science, San Francisco",
journal = "Plos One",
title = "New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide",
number = "6",
volume = "12",
doi = "10.1371/journal.pone.0179650"
}

Waisi, H. K., Janković, B., Janković, M., Nikolić, B., Dimkić, I., Lalević, B.,& Raičević, V.. (2017). New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide. in Plos One
Public Library Science, San Francisco., 12(6).
https://doi.org/10.1371/journal.pone.0179650

Waisi HK, Janković B, Janković M, Nikolić B, Dimkić I, Lalević B, Raičević V. New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide. in Plos One. 2017;12(6).
doi:10.1371/journal.pone.0179650 .

Waisi, Hadi K., Janković, Bojan, Janković, Marija, Nikolić, Bogdan, Dimkić, Ivica, Lalević, Blažo, Raičević, Vera, "New insights in dehydration stress behavior of two maize hybrids using advanced distributed reactivity model (DRM). Responses to the impact of 24-epibrassinolide" in Plos One, 12, no. 6 (2017),
https://doi.org/10.1371/journal.pone.0179650 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Jovanović, Ljubinko
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4247
AB  - Plant growth promoting (PGP) bacteria and yeasts play an important role in bioremediation processes. Thirty bacterial and ten yeast isolates were obtained from PAH and PCB contaminated soil with an aim of determining the presence of PGP mechanisms (production of ammonia, indoleacetic acid, siderophores and solubilization of inorganic phosphate). As a result, three bacterial (Serratia liquefaciens, Micrococcus sp. and Serratia sp.) and two yeast isolates (Candida utilis and Candida tropicalis) were recognized as PGP strains. Among them, Serratia sp. showed the highest indole production (25.5 μg/ml). Analyses of metal tolerance (Cu+2, Cr+6 and Ni+2) revealed that Serratia liquefaciens, Micrococcus sp., Serratia sp. and Candida tropicalis were capable to tolerate significant concentration of metals. As a result of this study several bacterial and yeast strains were attributed as potential plant growth promoters which can be applied in future remediation activities and environmental quality improvements.
AB  - Zemljište predstavlja dinamičan ekosistem naseljen brojnim mikroorganizmima među kojima su bakterije najbrojnije. Najveći broj je skoncentrisan u uskoj zoni zemljišta koja okružuje koren i koja se naziva rizosfera. Procenjuje se da 1-2% bakterija koje naseljavaju ovu zonu imaju sposobnost da promovišu rast biljaka (engl. plant growth promoting bacteria - PGPB). Pored bakterija, sve više je podataka da i zemljišni kvasci poseduju ove sposobnosti. Mikroorganizmi koji stimulišu rast biljaka koriste različite mehanizme kojima povećavaju dostupnost nutrijenata biljkama, regulišu njihov hormonski status kao i odnos prema biljnim patogenima. Bakterije i kvasci koji stimulišu rast biljaka igraju važnu ulogu i u procesima bioremedijacije. Trideset bakterijskih i deset izolata kvasaca je izolovano iz kontaminiranog zemljišta (PAH i PCB) i testirano na prisustvo mehanizama kojima se pospešuje rast biljaka. Cilj je bio doći do sojeva koji su prilagođeni na život u zagađenom zemljištu, a istovremeno imaju i potencijalno stimulativno dejstvo. Nakon izvršenih biohemijskih analiza (produkcija amonijaka, indol-sirćetne kiseline, siderofora, rastvaranje neorganskog fosfora) u stimulatore biljnog rasta svrstana su tri bakterijska (Serratia liquefaciens, Micrococcus sp. i Serratia sp.) i dva izolata kvasaca (Candida utilis i Candida tropicalis). Među njima najveću sposobnost produkcije indol-sirćetne kiseline je pokazao izolat Serratia sp. (25,5 μg/ml). Analize tolerancije na prisustvo teških metala (Cu+2, Cr+6 i Ni+2) pokazale su da su izolati Serratia liquefaciens, Micrococcus sp., Serratia sp. i Candida tropicalis sposobni da podnesu više koncentracije. Rezultati ovih istraživanja mogu imati praktičnu primenu u budućim remedijacionim aktivnostima i unapređenju kvaliteta životne sredine.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Isolation and characterization of bacteria and yeasts from contaminated soil
T1  - Izolacija i karakterizacija bakterija i kvasaca iz kontaminiranog zemljišta
EP  - 256
IS  - 3
SP  - 247
VL  - 61
DO  - 10.2298/JAS1603247K
ER  - 

@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Lalević, Blažo and Jovanović, Ljubinko and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "Plant growth promoting (PGP) bacteria and yeasts play an important role in bioremediation processes. Thirty bacterial and ten yeast isolates were obtained from PAH and PCB contaminated soil with an aim of determining the presence of PGP mechanisms (production of ammonia, indoleacetic acid, siderophores and solubilization of inorganic phosphate). As a result, three bacterial (Serratia liquefaciens, Micrococcus sp. and Serratia sp.) and two yeast isolates (Candida utilis and Candida tropicalis) were recognized as PGP strains. Among them, Serratia sp. showed the highest indole production (25.5 μg/ml). Analyses of metal tolerance (Cu+2, Cr+6 and Ni+2) revealed that Serratia liquefaciens, Micrococcus sp., Serratia sp. and Candida tropicalis were capable to tolerate significant concentration of metals. As a result of this study several bacterial and yeast strains were attributed as potential plant growth promoters which can be applied in future remediation activities and environmental quality improvements., Zemljište predstavlja dinamičan ekosistem naseljen brojnim mikroorganizmima među kojima su bakterije najbrojnije. Najveći broj je skoncentrisan u uskoj zoni zemljišta koja okružuje koren i koja se naziva rizosfera. Procenjuje se da 1-2% bakterija koje naseljavaju ovu zonu imaju sposobnost da promovišu rast biljaka (engl. plant growth promoting bacteria - PGPB). Pored bakterija, sve više je podataka da i zemljišni kvasci poseduju ove sposobnosti. Mikroorganizmi koji stimulišu rast biljaka koriste različite mehanizme kojima povećavaju dostupnost nutrijenata biljkama, regulišu njihov hormonski status kao i odnos prema biljnim patogenima. Bakterije i kvasci koji stimulišu rast biljaka igraju važnu ulogu i u procesima bioremedijacije. Trideset bakterijskih i deset izolata kvasaca je izolovano iz kontaminiranog zemljišta (PAH i PCB) i testirano na prisustvo mehanizama kojima se pospešuje rast biljaka. Cilj je bio doći do sojeva koji su prilagođeni na život u zagađenom zemljištu, a istovremeno imaju i potencijalno stimulativno dejstvo. Nakon izvršenih biohemijskih analiza (produkcija amonijaka, indol-sirćetne kiseline, siderofora, rastvaranje neorganskog fosfora) u stimulatore biljnog rasta svrstana su tri bakterijska (Serratia liquefaciens, Micrococcus sp. i Serratia sp.) i dva izolata kvasaca (Candida utilis i Candida tropicalis). Među njima najveću sposobnost produkcije indol-sirćetne kiseline je pokazao izolat Serratia sp. (25,5 μg/ml). Analize tolerancije na prisustvo teških metala (Cu+2, Cr+6 i Ni+2) pokazale su da su izolati Serratia liquefaciens, Micrococcus sp., Serratia sp. i Candida tropicalis sposobni da podnesu više koncentracije. Rezultati ovih istraživanja mogu imati praktičnu primenu u budućim remedijacionim aktivnostima i unapređenju kvaliteta životne sredine.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Isolation and characterization of bacteria and yeasts from contaminated soil, Izolacija i karakterizacija bakterija i kvasaca iz kontaminiranog zemljišta",
pages = "256-247",
number = "3",
volume = "61",
doi = "10.2298/JAS1603247K"
}

Karličić, V., Radić, D., Jovičić-Petrović, J., Lalević, B., Jovanović, L., Kiković, D.,& Raičević, V.. (2016). Isolation and characterization of bacteria and yeasts from contaminated soil. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 61(3), 247-256.
https://doi.org/10.2298/JAS1603247K

Karličić V, Radić D, Jovičić-Petrović J, Lalević B, Jovanović L, Kiković D, Raičević V. Isolation and characterization of bacteria and yeasts from contaminated soil. in Journal of Agricultural Sciences (Belgrade). 2016;61(3):247-256.
doi:10.2298/JAS1603247K .

Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Lalević, Blažo, Jovanović, Ljubinko, Kiković, Dragan, Raičević, Vera, "Isolation and characterization of bacteria and yeasts from contaminated soil" in Journal of Agricultural Sciences (Belgrade), 61, no. 3 (2016):247-256,
https://doi.org/10.2298/JAS1603247K . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Golubović-Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4307
AB  - Surface mining causes major destruction of natural landscapes and ecosystems. The most fertile, surface soil layer is lost permanently, together with vegetation, wildlife, and micro flora. Post-mining areas are characterized with diverse edaphic, topographic, hydrographic conditions, which complicate land restoration. Successful establishment of forest ecosystems on such land depends mostly on selection of tree species. The chosen plants must be capable of tolerating a wide range of acidity, fertility, moisture, and have potential to ameliorate such substrates for more demanding species. But, reforestation of heavily damaged ecosystems, such as post-mining areas, demands a new approach in seedlings production. This new approach takes into account specific requirements of habitat and integrates them into 'targeted production of planting material'. A good strategy for successful reforestation of post-mining areas is the input of organic matter (compost, mulch). Also, current knowledge and experiences emphasize the potential of beneficial microorganisms such as, mycorrhizal fungi (MF) and plant growth promoting rhizobacteria (PGPR). The majority of studies that deal with beneficial interactions between trees and microorganisms are focused on the mycorrhiza, while plant growth promoting rhizobacteria are less present in silviculture. In this study, the focus is on the reforestation challenges of two mining basins, Majdanpek and Kolubara and suggests beneficial microorganisms as potential solution. The study presents results of several years' researches on plant response to the presence of mycorrhizal fungi and PGPR. The substrates used for plant growth were Majdanpek and Kolubara mine deposals. Mycorrhizal seedlings were grown in Majdanpek mine deposal, and at the end of the experiment they had 30% higher biomass in comparison to control (seedlings without mycorrhiza). Seedlings linked with fungi had a higher survival rate. Deposals from Kolubara Mining Basin were used as a substrate for seedlings inoculated with PGPR. In the first experiment, Scots pine and Norway spruce were inoculated with Azotobacter chroococcum, Bacillus megaterium, B. circulans, B. licheniformis, B. pumilus, B. amyloliquefaciens. Inoculation resulted with higher biomass production (Scots pine 43%, Norway spruce 34%). Similar results were obtained in the second experiment where Scots pine and black locust were inoculated with Bacillus licheniformis, Aeromonas hydrophila, Pseudomonas putida and Burkholderia cepacia. Both species had higher biomass (around 20%) in comparison to un-inoculated control. The results confirmed the fact that early establishment and successful growth of vegetation on devastated areas depends on the presence and activity of soil microbes. Microorganisms as a 'nature's solution' pose the potential to alleviate reforestation challenges of anthropogenic devastated landscapes. Their presence and activity is crucial for ecosystem stability. In areas with compromised balance, their introduction is justified action for achieving the goal of long term ecosystem sustainability.
PB  - SPS Reforesta, Beograd
T2  - Reforesta
T1  - The alleviation of reforestation challenges by beneficial soil microorganisms
EP  - 260
IS  - 1
SP  - 238
VL  - 1
DO  - 10.21750/REFOR.1.12.12
ER  - 

@article{
author = "Karličić, Vera and Golubović-Ćurguz, Vesna and Raičević, Vera",
year = "2016",
abstract = "Surface mining causes major destruction of natural landscapes and ecosystems. The most fertile, surface soil layer is lost permanently, together with vegetation, wildlife, and micro flora. Post-mining areas are characterized with diverse edaphic, topographic, hydrographic conditions, which complicate land restoration. Successful establishment of forest ecosystems on such land depends mostly on selection of tree species. The chosen plants must be capable of tolerating a wide range of acidity, fertility, moisture, and have potential to ameliorate such substrates for more demanding species. But, reforestation of heavily damaged ecosystems, such as post-mining areas, demands a new approach in seedlings production. This new approach takes into account specific requirements of habitat and integrates them into 'targeted production of planting material'. A good strategy for successful reforestation of post-mining areas is the input of organic matter (compost, mulch). Also, current knowledge and experiences emphasize the potential of beneficial microorganisms such as, mycorrhizal fungi (MF) and plant growth promoting rhizobacteria (PGPR). The majority of studies that deal with beneficial interactions between trees and microorganisms are focused on the mycorrhiza, while plant growth promoting rhizobacteria are less present in silviculture. In this study, the focus is on the reforestation challenges of two mining basins, Majdanpek and Kolubara and suggests beneficial microorganisms as potential solution. The study presents results of several years' researches on plant response to the presence of mycorrhizal fungi and PGPR. The substrates used for plant growth were Majdanpek and Kolubara mine deposals. Mycorrhizal seedlings were grown in Majdanpek mine deposal, and at the end of the experiment they had 30% higher biomass in comparison to control (seedlings without mycorrhiza). Seedlings linked with fungi had a higher survival rate. Deposals from Kolubara Mining Basin were used as a substrate for seedlings inoculated with PGPR. In the first experiment, Scots pine and Norway spruce were inoculated with Azotobacter chroococcum, Bacillus megaterium, B. circulans, B. licheniformis, B. pumilus, B. amyloliquefaciens. Inoculation resulted with higher biomass production (Scots pine 43%, Norway spruce 34%). Similar results were obtained in the second experiment where Scots pine and black locust were inoculated with Bacillus licheniformis, Aeromonas hydrophila, Pseudomonas putida and Burkholderia cepacia. Both species had higher biomass (around 20%) in comparison to un-inoculated control. The results confirmed the fact that early establishment and successful growth of vegetation on devastated areas depends on the presence and activity of soil microbes. Microorganisms as a 'nature's solution' pose the potential to alleviate reforestation challenges of anthropogenic devastated landscapes. Their presence and activity is crucial for ecosystem stability. In areas with compromised balance, their introduction is justified action for achieving the goal of long term ecosystem sustainability.",
publisher = "SPS Reforesta, Beograd",
journal = "Reforesta",
title = "The alleviation of reforestation challenges by beneficial soil microorganisms",
pages = "260-238",
number = "1",
volume = "1",
doi = "10.21750/REFOR.1.12.12"
}

Karličić, V., Golubović-Ćurguz, V.,& Raičević, V.. (2016). The alleviation of reforestation challenges by beneficial soil microorganisms. in Reforesta
SPS Reforesta, Beograd., 1(1), 238-260.
https://doi.org/10.21750/REFOR.1.12.12

Karličić V, Golubović-Ćurguz V, Raičević V. The alleviation of reforestation challenges by beneficial soil microorganisms. in Reforesta. 2016;1(1):238-260.
doi:10.21750/REFOR.1.12.12 .

Karličić, Vera, Golubović-Ćurguz, Vesna, Raičević, Vera, "The alleviation of reforestation challenges by beneficial soil microorganisms" in Reforesta, 1, no. 1 (2016):238-260,
https://doi.org/10.21750/REFOR.1.12.12 . .

TY  - JOUR
AU  - Atanasković, Iva M.
AU  - Jovičić-Petrović, Jelena
AU  - Biocanin, Marjan B.
AU  - Karličić, Vera
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4164
AB  - Bioremediation is promising technology for dealing with oil hydrocarbons contamination. In this research growth kinetics and oil biodegradation efficiency of Pseudomonas luteola PRO23, isolated from crude oil-contaminated soil samples, were investigated under different concentrations (5, 10 and 20 g/L) of light and heavy crude oil. More efficient biodegradation and more rapid adaptation and cell growth were obtained in conditions with light oil. The 5 to 10 g/L upgrade of light oil concentration stimulated the microbial growth and the biodegradation efficiency. Further upgrade of light oil concentration and the upgrade of heavy oil concentration both inhibited the microbial growth, as well as biodegradation process. Aminoglycosides stimulated biosurfactant production in P. luteola in the range of sub-inhibitory concentrations (0.3125, 0.625 mu g/mL). Aminoglycosides also induced biofilm formation. The production of biosurfactants was the most intense during lag phase and continues until stationary phase. Aminoglycosides also induced changes in P. luteola growth kinetics. In the presence of aminoglycosides this strain degraded 82% of diesel for 96 h. These results indicated that P. luteola PRO23 potentially can be used in bioremediation of crude oil-contaminated environments and that aminoglycosides could stimulate this process.
PB  - Savez hemijskih inženjera, Beograd
T2  - HEMIJSKA INDUSTRIJA
T1  - Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23
EP  - 150
IS  - 2
SP  - 143
VL  - 70
DO  - 10.2298/HEMIND141127020A
ER  - 

@article{
author = "Atanasković, Iva M. and Jovičić-Petrović, Jelena and Biocanin, Marjan B. and Karličić, Vera and Raičević, Vera and Lalević, Blažo",
year = "2016",
abstract = "Bioremediation is promising technology for dealing with oil hydrocarbons contamination. In this research growth kinetics and oil biodegradation efficiency of Pseudomonas luteola PRO23, isolated from crude oil-contaminated soil samples, were investigated under different concentrations (5, 10 and 20 g/L) of light and heavy crude oil. More efficient biodegradation and more rapid adaptation and cell growth were obtained in conditions with light oil. The 5 to 10 g/L upgrade of light oil concentration stimulated the microbial growth and the biodegradation efficiency. Further upgrade of light oil concentration and the upgrade of heavy oil concentration both inhibited the microbial growth, as well as biodegradation process. Aminoglycosides stimulated biosurfactant production in P. luteola in the range of sub-inhibitory concentrations (0.3125, 0.625 mu g/mL). Aminoglycosides also induced biofilm formation. The production of biosurfactants was the most intense during lag phase and continues until stationary phase. Aminoglycosides also induced changes in P. luteola growth kinetics. In the presence of aminoglycosides this strain degraded 82% of diesel for 96 h. These results indicated that P. luteola PRO23 potentially can be used in bioremediation of crude oil-contaminated environments and that aminoglycosides could stimulate this process.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "HEMIJSKA INDUSTRIJA",
title = "Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23",
pages = "150-143",
number = "2",
volume = "70",
doi = "10.2298/HEMIND141127020A"
}

Atanasković, I. M., Jovičić-Petrović, J., Biocanin, M. B., Karličić, V., Raičević, V.,& Lalević, B.. (2016). Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23. in HEMIJSKA INDUSTRIJA
Savez hemijskih inženjera, Beograd., 70(2), 143-150.
https://doi.org/10.2298/HEMIND141127020A

Atanasković IM, Jovičić-Petrović J, Biocanin MB, Karličić V, Raičević V, Lalević B. Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23. in HEMIJSKA INDUSTRIJA. 2016;70(2):143-150.
doi:10.2298/HEMIND141127020A .

Atanasković, Iva M., Jovičić-Petrović, Jelena, Biocanin, Marjan B., Karličić, Vera, Raičević, Vera, Lalević, Blažo, "Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23" in HEMIJSKA INDUSTRIJA, 70, no. 2 (2016):143-150,
https://doi.org/10.2298/HEMIND141127020A . .

TY  - JOUR
AU  - Pavlović, Vera P.
AU  - Radić, Danka
AU  - Karličić, Vera
AU  - Lalević, Blažo
AU  - Lević, Steva
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4232
AB  - In this paper, two isolates of yeasts, members of the genus Candida, have been examined using Raman spectroscopy. The yeasts were isolated from soils sampled at the town park in Tivat (Montenegro) and experimental farm Radmilovac (Serbia), by the accumulation method. The pure cultures of yeasts were identified by the API 20C AUX system. The presence of Candida guilliermondii and Candida utilis was noted. The Raman spectra, originating from lipids, amides, proteins, carbohydrates, aromatic amino acids and the nucleotide bases, were analyzed in the wide range of the wave numbers from 500 to 3200 cm-1. It was noticed that peaks which correspond to the lipid fraction were more pronounced in the case of Candida guilliermondii compared to Candida utilis. This may represent a specific response of the yeast species to stress conditions.
AB  - U ovom radu su Raman spektroskopijom ispitivana dva izolata kvasaca iz roda Candida. Izolacija kvasaca je izvršena iz zemljišta uzetog iz gradskog parka u Tivtu (Crna Gora) i sa oglednog dobra Radmilovac (Poljoprivredni fakultet, Beograd, Srbija), metodom nakupljanja. Dobijene čiste kulture kvasaca identifikovane su primenom API AUX 20C sistema (bioMerieux-Vitek). Konstatovano je prisustvo vrsta Candida guilliermondii i Candida utilis. Analizirani su Raman spektri koji potiču od lipida, amida, proteina, ugljenih hidrata, aromatičnih aminokiselina i nukleotidnih baza, u širokoj oblasti talasnih brojeva, od 500 do 3200 cm-1. Utvrđeno je da su kod kvasca Candida guilliermondii pikovi koji odgovaraju frakciji lipida bitno izraženiji nego kod Candida utilis, što može predstavljati specifičan odgovor date vrste kvasca na stres.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Raman spectroscopy and determination of soil yeasts
T1  - Raman spektroskopija i determinacija zemljišnih kvasaca
EP  - 459
IS  - 3
SP  - 455
VL  - 57
DO  - 10.5937/ZasMat1603455P
ER  - 

@article{
author = "Pavlović, Vera P. and Radić, Danka and Karličić, Vera and Lalević, Blažo and Lević, Steva and Raičević, Vera",
year = "2016",
abstract = "In this paper, two isolates of yeasts, members of the genus Candida, have been examined using Raman spectroscopy. The yeasts were isolated from soils sampled at the town park in Tivat (Montenegro) and experimental farm Radmilovac (Serbia), by the accumulation method. The pure cultures of yeasts were identified by the API 20C AUX system. The presence of Candida guilliermondii and Candida utilis was noted. The Raman spectra, originating from lipids, amides, proteins, carbohydrates, aromatic amino acids and the nucleotide bases, were analyzed in the wide range of the wave numbers from 500 to 3200 cm-1. It was noticed that peaks which correspond to the lipid fraction were more pronounced in the case of Candida guilliermondii compared to Candida utilis. This may represent a specific response of the yeast species to stress conditions., U ovom radu su Raman spektroskopijom ispitivana dva izolata kvasaca iz roda Candida. Izolacija kvasaca je izvršena iz zemljišta uzetog iz gradskog parka u Tivtu (Crna Gora) i sa oglednog dobra Radmilovac (Poljoprivredni fakultet, Beograd, Srbija), metodom nakupljanja. Dobijene čiste kulture kvasaca identifikovane su primenom API AUX 20C sistema (bioMerieux-Vitek). Konstatovano je prisustvo vrsta Candida guilliermondii i Candida utilis. Analizirani su Raman spektri koji potiču od lipida, amida, proteina, ugljenih hidrata, aromatičnih aminokiselina i nukleotidnih baza, u širokoj oblasti talasnih brojeva, od 500 do 3200 cm-1. Utvrđeno je da su kod kvasca Candida guilliermondii pikovi koji odgovaraju frakciji lipida bitno izraženiji nego kod Candida utilis, što može predstavljati specifičan odgovor date vrste kvasca na stres.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Raman spectroscopy and determination of soil yeasts, Raman spektroskopija i determinacija zemljišnih kvasaca",
pages = "459-455",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603455P"
}

Pavlović, V. P., Radić, D., Karličić, V., Lalević, B., Lević, S.,& Raičević, V.. (2016). Raman spectroscopy and determination of soil yeasts. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 455-459.
https://doi.org/10.5937/ZasMat1603455P

Pavlović VP, Radić D, Karličić V, Lalević B, Lević S, Raičević V. Raman spectroscopy and determination of soil yeasts. in Zaštita materijala. 2016;57(3):455-459.
doi:10.5937/ZasMat1603455P .

Pavlović, Vera P., Radić, Danka, Karličić, Vera, Lalević, Blažo, Lević, Steva, Raičević, Vera, "Raman spectroscopy and determination of soil yeasts" in Zaštita materijala, 57, no. 3 (2016):455-459,
https://doi.org/10.5937/ZasMat1603455P . .

TY  - JOUR
AU  - Ilić, Dora
AU  - Radić, Danka
AU  - Karličić, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Kiković, Dragan
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4233
AB  - Except of benefits for human population, several industrial activities have a negative consequences i.e. increasing of heavy metal content and changes in diversity of living organisms in environment. The aim of this work was to examinated the influence of high content of heavy metals on microbial diversity of soil. Researches was conducted at locations of former factory 'Rog' (Ljubljana, Republic of Slovenia), by taking of four samples of contaminated soil, while the control was uncontaminated soil sample. Determination of microbial diversity (abundance of bacteria, fungi and actinomycetes) was performed using the standard methodology. The results of research showed that abundance of microorganisms depends on location and presence/absence of contamination. In control treatment abundance of all examinated groups of microorganisms was higher compared with other samples. The bacterial number in contaminated samples was from 1.21 to 6.88x106 CFU/g, the number of fungi was from 1,08 to 30,30x103 CFU/g, while actinomycetes number from 302,15 to 381,82x103 CFU/g. In control treatment, bacterial number was 17,45x106 CFU/g, fungi number 54,02x103 CFU/g and actinomycetes number 481,91x103 CFU/g. These results confirms the importance of microbial diversity research on contaminated locations and potential applications of bioremediation technologies on locations of former factory 'Rog'.
AB  - Mnogobrojne industrijske aktivnosti, osim koristi za ljudsku populaciju, imale su za posledicu povećanje sadržaja teških metala i promene diverziteta živih organizama u životnoj sredini. Cilj ovog rada bio je ispitivanje uticaja visokih koncentracija teških metala na mikrobni diverzitet zemljišta. Istraživanja su obavljena na području bivše fabrike 'Rog' (Ljubljana, Republika Slovenija), gde su uzeta četiri uzorka kontaminiranog zemljišta, dok je kontrolni uzorak predstavljao nekontaminirano zemljište. Ispitivanje mikrobnog diverziteta (brojnost bakterija, gljiva i aktinomiceta) obavljeno je primenom standardnih metoda. Rezultati istraživanja ukazuju da je brojnost mikroorganizama zavisila od lokacije i prusustva/odsustva kontaminacije. U kontrolnoj varijanti brojnost svih ispitivanih grupa mikroorganizama bila je veća u odnosu na ostale uzorke. Brojnost bakterija u kontaminiranim uzorcima iznosila je od 1,21-6,88x106 CFU/g, gljiva 1,08-30,30x103 CFU/g i aktinomiceta 302,15-381,82x103 CFU/g. U kontrolnoj varijanti broj bakterija iznosio je 17,45x106 CFU/g, gljiva 54,02x103 CFU/g i aktinomiceta 481,91x103 CFU/g. Ovi rezultati ukuzuju na značaj ispitivanja mikrobnog diverziteta na kontaminiranim lokacijama i predstavljaju osnovu za potencijalne aplikacije bioremedijacionih tehnologija na području bivše fabrike 'Rog'.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Microbial diversity of soil contaminated with high content of heavy metals
T1  - Mikrobni diverzitet zemljišta kontaminiranog visokim sadržajem teških metala
EP  - 387
IS  - 3
SP  - 383
VL  - 57
DO  - 10.5937/ZasMat1603383I
ER  - 

@article{
author = "Ilić, Dora and Radić, Danka and Karličić, Vera and Jovičić-Petrović, Jelena and Kiković, Dragan and Lalević, Blažo and Raičević, Vera",
year = "2016",
abstract = "Except of benefits for human population, several industrial activities have a negative consequences i.e. increasing of heavy metal content and changes in diversity of living organisms in environment. The aim of this work was to examinated the influence of high content of heavy metals on microbial diversity of soil. Researches was conducted at locations of former factory 'Rog' (Ljubljana, Republic of Slovenia), by taking of four samples of contaminated soil, while the control was uncontaminated soil sample. Determination of microbial diversity (abundance of bacteria, fungi and actinomycetes) was performed using the standard methodology. The results of research showed that abundance of microorganisms depends on location and presence/absence of contamination. In control treatment abundance of all examinated groups of microorganisms was higher compared with other samples. The bacterial number in contaminated samples was from 1.21 to 6.88x106 CFU/g, the number of fungi was from 1,08 to 30,30x103 CFU/g, while actinomycetes number from 302,15 to 381,82x103 CFU/g. In control treatment, bacterial number was 17,45x106 CFU/g, fungi number 54,02x103 CFU/g and actinomycetes number 481,91x103 CFU/g. These results confirms the importance of microbial diversity research on contaminated locations and potential applications of bioremediation technologies on locations of former factory 'Rog'., Mnogobrojne industrijske aktivnosti, osim koristi za ljudsku populaciju, imale su za posledicu povećanje sadržaja teških metala i promene diverziteta živih organizama u životnoj sredini. Cilj ovog rada bio je ispitivanje uticaja visokih koncentracija teških metala na mikrobni diverzitet zemljišta. Istraživanja su obavljena na području bivše fabrike 'Rog' (Ljubljana, Republika Slovenija), gde su uzeta četiri uzorka kontaminiranog zemljišta, dok je kontrolni uzorak predstavljao nekontaminirano zemljište. Ispitivanje mikrobnog diverziteta (brojnost bakterija, gljiva i aktinomiceta) obavljeno je primenom standardnih metoda. Rezultati istraživanja ukazuju da je brojnost mikroorganizama zavisila od lokacije i prusustva/odsustva kontaminacije. U kontrolnoj varijanti brojnost svih ispitivanih grupa mikroorganizama bila je veća u odnosu na ostale uzorke. Brojnost bakterija u kontaminiranim uzorcima iznosila je od 1,21-6,88x106 CFU/g, gljiva 1,08-30,30x103 CFU/g i aktinomiceta 302,15-381,82x103 CFU/g. U kontrolnoj varijanti broj bakterija iznosio je 17,45x106 CFU/g, gljiva 54,02x103 CFU/g i aktinomiceta 481,91x103 CFU/g. Ovi rezultati ukuzuju na značaj ispitivanja mikrobnog diverziteta na kontaminiranim lokacijama i predstavljaju osnovu za potencijalne aplikacije bioremedijacionih tehnologija na području bivše fabrike 'Rog'.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Microbial diversity of soil contaminated with high content of heavy metals, Mikrobni diverzitet zemljišta kontaminiranog visokim sadržajem teških metala",
pages = "387-383",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603383I"
}

Ilić, D., Radić, D., Karličić, V., Jovičić-Petrović, J., Kiković, D., Lalević, B.,& Raičević, V.. (2016). Microbial diversity of soil contaminated with high content of heavy metals. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 383-387.
https://doi.org/10.5937/ZasMat1603383I

Ilić D, Radić D, Karličić V, Jovičić-Petrović J, Kiković D, Lalević B, Raičević V. Microbial diversity of soil contaminated with high content of heavy metals. in Zaštita materijala. 2016;57(3):383-387.
doi:10.5937/ZasMat1603383I .

Ilić, Dora, Radić, Danka, Karličić, Vera, Jovičić-Petrović, Jelena, Kiković, Dragan, Lalević, Blažo, Raičević, Vera, "Microbial diversity of soil contaminated with high content of heavy metals" in Zaštita materijala, 57, no. 3 (2016):383-387,
https://doi.org/10.5937/ZasMat1603383I . .

TY  - JOUR
AU  - Jovičić-Petrović, Jelena
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4166
AB  - In this paper we report on the isolation and identification of three filamentous fungi from grape marc, and antifungal effect of their cell-free culture filtrates on the growth of Botrytis cinerea, causal agent of gray mold. Grape marc is a waste material that has been used as soil amendment in sustainable agriculture. Isolates originating from grape marc were identified on the basis of morphological features and internal transcribed spacer rDNA or beta-tubulin gene sequencing. The presence of three different species, Penicillium paneum, Penicillium chrysogenum and Aspergillus fumigatus has been detected expressing different effect on the growth of B. cinerea. The effect of crude culture filtrates of selected fungi on B. cinerea growth was tested. Heat sensitivity of the established inhibition effect was examined by autoclaving the crude culture filtrate prior to testing. Additional aim was to determine whether antifungal effect was influenced by previous exposure to B. cinerea in dual liquid cultures. Crude culture filtrate of A. fumigatus K16/2 showed the lowest suppression of B. cinerea growth. A maximal percentage inhibition achieved within the study was 38.2%, 39.8% and 23.8 for crude filtrates of P. paneum K7/1, P. chrysogenum K11/1 and A. fumigatus K16/2, respectively. Presence of B. cinerea in dual liquid culture induced significant increase in antifungal capacity of the culture filtrates in comparison to pure culture filtrates of the chosen isolates. The antifungal activity of all of the isolates' culture filtrates retained after heat treatment suggesting the presence of some thermostable antifungal metabolites. The results indicate the complexity and specificity of the interaction between filamentous fungi and B. cinerea. Grape marc is a good source for isolation od B. cinerea fungal antagonists and their antifungal metabolites. Specificity of fungal-fungal interactions suggests that further research on the antagonistic mechanisms and factors affecting them should be studied separately for each pair of antagonists.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea
EP  - 48
IS  - 1
SP  - 37
VL  - 48
DO  - 10.2298/GENSR1601037J
ER  - 

@article{
author = "Jovičić-Petrović, Jelena and Stanković, Ivana and Bulajić, Aleksandra and Krstić, Branka and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "In this paper we report on the isolation and identification of three filamentous fungi from grape marc, and antifungal effect of their cell-free culture filtrates on the growth of Botrytis cinerea, causal agent of gray mold. Grape marc is a waste material that has been used as soil amendment in sustainable agriculture. Isolates originating from grape marc were identified on the basis of morphological features and internal transcribed spacer rDNA or beta-tubulin gene sequencing. The presence of three different species, Penicillium paneum, Penicillium chrysogenum and Aspergillus fumigatus has been detected expressing different effect on the growth of B. cinerea. The effect of crude culture filtrates of selected fungi on B. cinerea growth was tested. Heat sensitivity of the established inhibition effect was examined by autoclaving the crude culture filtrate prior to testing. Additional aim was to determine whether antifungal effect was influenced by previous exposure to B. cinerea in dual liquid cultures. Crude culture filtrate of A. fumigatus K16/2 showed the lowest suppression of B. cinerea growth. A maximal percentage inhibition achieved within the study was 38.2%, 39.8% and 23.8 for crude filtrates of P. paneum K7/1, P. chrysogenum K11/1 and A. fumigatus K16/2, respectively. Presence of B. cinerea in dual liquid culture induced significant increase in antifungal capacity of the culture filtrates in comparison to pure culture filtrates of the chosen isolates. The antifungal activity of all of the isolates' culture filtrates retained after heat treatment suggesting the presence of some thermostable antifungal metabolites. The results indicate the complexity and specificity of the interaction between filamentous fungi and B. cinerea. Grape marc is a good source for isolation od B. cinerea fungal antagonists and their antifungal metabolites. Specificity of fungal-fungal interactions suggests that further research on the antagonistic mechanisms and factors affecting them should be studied separately for each pair of antagonists.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea",
pages = "48-37",
number = "1",
volume = "48",
doi = "10.2298/GENSR1601037J"
}

Jovičić-Petrović, J., Stanković, I., Bulajić, A., Krstić, B., Kiković, D.,& Raičević, V.. (2016). Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea. in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 37-48.
https://doi.org/10.2298/GENSR1601037J

Jovičić-Petrović J, Stanković I, Bulajić A, Krstić B, Kiković D, Raičević V. Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea. in Genetika. 2016;48(1):37-48.
doi:10.2298/GENSR1601037J .

Jovičić-Petrović, Jelena, Stanković, Ivana, Bulajić, Aleksandra, Krstić, Branka, Kiković, Dragan, Raičević, Vera, "Filamentous fungi isolated from grape marc as antagonists of botrytis cinerea" in Genetika, 48, no. 1 (2016):37-48,
https://doi.org/10.2298/GENSR1601037J . .

TY  - JOUR
AU  - Jovičić-Petrović, Jelena
AU  - Jeremić, Sanja
AU  - Vucković, Ivan
AU  - Vojnović, Sandra
AU  - Bulajić, Aleksandra
AU  - Raičević, Vera
AU  - Nikodinović-Runić, Jasmina
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4175
AB  - Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, beta-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/ 5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum
EP  - 289
IS  - 2
SP  - 279
VL  - 68
DO  - 10.2298/ABS150602016J
ER  - 

@article{
author = "Jovičić-Petrović, Jelena and Jeremić, Sanja and Vucković, Ivan and Vojnović, Sandra and Bulajić, Aleksandra and Raičević, Vera and Nikodinović-Runić, Jasmina",
year = "2016",
abstract = "Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, beta-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/ 5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum",
pages = "289-279",
number = "2",
volume = "68",
doi = "10.2298/ABS150602016J"
}

Jovičić-Petrović, J., Jeremić, S., Vucković, I., Vojnović, S., Bulajić, A., Raičević, V.,& Nikodinović-Runić, J.. (2016). Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 68(2), 279-289.
https://doi.org/10.2298/ABS150602016J

Jovičić-Petrović J, Jeremić S, Vucković I, Vojnović S, Bulajić A, Raičević V, Nikodinović-Runić J. Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum. in Archives of Biological Sciences. 2016;68(2):279-289.
doi:10.2298/ABS150602016J .

Jovičić-Petrović, Jelena, Jeremić, Sanja, Vucković, Ivan, Vojnović, Sandra, Bulajić, Aleksandra, Raičević, Vera, Nikodinović-Runić, Jasmina, "Aspergillus piperis a/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen pythium aphanidermatum" in Archives of Biological Sciences, 68, no. 2 (2016):279-289,
https://doi.org/10.2298/ABS150602016J . .

TY  - JOUR
AU  - Vujović, Bojana
AU  - Rudić, Željka
AU  - Kljujev, Igor
AU  - Rajković, Dobrica
AU  - Božić, Mile
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4228
AB  - Biofilm formation is one of the main characteristic of Pseudomonas aeruginosa strains. This ability provide bacteria to survive in different, usually restrictivesss environmental conditions. In the same time, biofilm forming is a risk in a many field of industry, mainly in food industry. It is known that diverse P. aeruginosa strains from various environmental sources have different potention to form biofilms. In this paper it is examinated the potention to form biofilm four isolates of P. aeruginosa and based of this results, tested isolates have classificated into four groups. Biofilm formation tested in microtitar plates during the 24 hours at changed conditions of temperature (37°C and 22°C), pH (6 and 7) and concentration of NaCl (1% and 2%). Obtained results have shown that isolated P. aeruginosa have unsimilar ability to form biofilms. Biomass of formed biofilm was greater at 22°C than at 37°C. Also, acid conditions and higher concentration of salt had no inhibitory effect to biofilm forming process.
AB  - Stvaranje biofilmova predstavlja jednu od glavnih osobina Pseudomonas aeruginosa koja omogućava ovoj bakteriji preživljavanje u različitim, često nepovoljnim uslovima životne sredine. Međutim, formiranje biofilmova predstavlja i faktor rizika u mnogim oblastima industrije, posebno prehrambene, pošto prisustvo čvrstih površina pruža dobre uslove za formiranje biofilma. Kako je poznato da izolati P. aeruginosa koji potiču iz različitih uzoraka poreklom iz životne sredine imaju različit potencijal da formiraju biofilmove, u ovom radu je ispitana sposobnost formiranja biofilmova četiri izolata ove bakterije i izvršena njihova klasifikacija u odnosu na ovu osobinu. Primenjen je statički test u mikrotitar pločama u trajanju od 24 sata u različitim uslovima temperature (37°C i 22°C), pH (6 i 7) i koncentracije NaCl (1 i 2%). Dobijeni rezultati su pokazali da izolati P. aeruginosa imaju različitu sposobnost da formiraju biofilmove, da je masa formiranih biofilmova bila veća na 22°C, kao i da promenjeni uslovi sredine nisu inhibirali stvaranje biofilmova.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Potential of pseudomonas aeruginosa environmental isolates for biofilm formation
T1  - Potencijal formiranja biofilmova pseudomonas aeruginosa iz životne sredine
EP  - 454
IS  - 3
SP  - 449
VL  - 57
DO  - 10.5937/ZasMat1603449V
ER  - 

@article{
author = "Vujović, Bojana and Rudić, Željka and Kljujev, Igor and Rajković, Dobrica and Božić, Mile and Raičević, Vera",
year = "2016",
abstract = "Biofilm formation is one of the main characteristic of Pseudomonas aeruginosa strains. This ability provide bacteria to survive in different, usually restrictivesss environmental conditions. In the same time, biofilm forming is a risk in a many field of industry, mainly in food industry. It is known that diverse P. aeruginosa strains from various environmental sources have different potention to form biofilms. In this paper it is examinated the potention to form biofilm four isolates of P. aeruginosa and based of this results, tested isolates have classificated into four groups. Biofilm formation tested in microtitar plates during the 24 hours at changed conditions of temperature (37°C and 22°C), pH (6 and 7) and concentration of NaCl (1% and 2%). Obtained results have shown that isolated P. aeruginosa have unsimilar ability to form biofilms. Biomass of formed biofilm was greater at 22°C than at 37°C. Also, acid conditions and higher concentration of salt had no inhibitory effect to biofilm forming process., Stvaranje biofilmova predstavlja jednu od glavnih osobina Pseudomonas aeruginosa koja omogućava ovoj bakteriji preživljavanje u različitim, često nepovoljnim uslovima životne sredine. Međutim, formiranje biofilmova predstavlja i faktor rizika u mnogim oblastima industrije, posebno prehrambene, pošto prisustvo čvrstih površina pruža dobre uslove za formiranje biofilma. Kako je poznato da izolati P. aeruginosa koji potiču iz različitih uzoraka poreklom iz životne sredine imaju različit potencijal da formiraju biofilmove, u ovom radu je ispitana sposobnost formiranja biofilmova četiri izolata ove bakterije i izvršena njihova klasifikacija u odnosu na ovu osobinu. Primenjen je statički test u mikrotitar pločama u trajanju od 24 sata u različitim uslovima temperature (37°C i 22°C), pH (6 i 7) i koncentracije NaCl (1 i 2%). Dobijeni rezultati su pokazali da izolati P. aeruginosa imaju različitu sposobnost da formiraju biofilmove, da je masa formiranih biofilmova bila veća na 22°C, kao i da promenjeni uslovi sredine nisu inhibirali stvaranje biofilmova.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Potential of pseudomonas aeruginosa environmental isolates for biofilm formation, Potencijal formiranja biofilmova pseudomonas aeruginosa iz životne sredine",
pages = "454-449",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603449V"
}

Vujović, B., Rudić, Ž., Kljujev, I., Rajković, D., Božić, M.,& Raičević, V.. (2016). Potential of pseudomonas aeruginosa environmental isolates for biofilm formation. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 449-454.
https://doi.org/10.5937/ZasMat1603449V

Vujović B, Rudić Ž, Kljujev I, Rajković D, Božić M, Raičević V. Potential of pseudomonas aeruginosa environmental isolates for biofilm formation. in Zaštita materijala. 2016;57(3):449-454.
doi:10.5937/ZasMat1603449V .

Vujović, Bojana, Rudić, Željka, Kljujev, Igor, Rajković, Dobrica, Božić, Mile, Raičević, Vera, "Potential of pseudomonas aeruginosa environmental isolates for biofilm formation" in Zaštita materijala, 57, no. 3 (2016):449-454,
https://doi.org/10.5937/ZasMat1603449V . .