Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bact...eria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.
Keywords:
Contamination vegetables / Salmonella spp. / Irrigation water / FISH / CLSM
TY - JOUR
AU - Kljujev, Igor
AU - Raičević, Vera
AU - Vujović, Bojana
AU - Rothballer, Michael
AU - Schmid, Michael
PY - 2018
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4720
AB - Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.
PB - Academic Press Ltd- Elsevier Science Ltd, London
T2 - Microbial Pathogenesis
T1 - Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production
EP - 207
SP - 199
VL - 115
DO - 10.1016/j.micpath.2017.12.020
UR - conv_6198
ER -
@article{
author = "Kljujev, Igor and Raičević, Vera and Vujović, Bojana and Rothballer, Michael and Schmid, Michael",
year = "2018",
abstract = "Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production",
pages = "207-199",
volume = "115",
doi = "10.1016/j.micpath.2017.12.020",
url = "conv_6198"
}
Kljujev, I., Raičević, V., Vujović, B., Rothballer, M.,& Schmid, M.. (2018). Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 115, 199-207.
https://doi.org/10.1016/j.micpath.2017.12.020
conv_6198
Kljujev I, Raičević V, Vujović B, Rothballer M, Schmid M. Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis. 2018;115:199-207.
doi:10.1016/j.micpath.2017.12.020
conv_6198 .
Kljujev, Igor, Raičević, Vera, Vujović, Bojana, Rothballer, Michael, Schmid, Michael, "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production" in Microbial Pathogenesis, 115 (2018):199-207,
https://doi.org/10.1016/j.micpath.2017.12.020 .,
conv_6198 .
