TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Subotić, Srđan
AU  - Višnjić-Jeftić, Željka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6354
AB  - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Ecotoxicology
T2  - Ecotoxicology
T1  - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
DO  - 10.1007/s10646-023-02663-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić-Jeftić, Željka and Rašković, Božidar and Poleksić, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2023",
abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Ecotoxicology, Ecotoxicology",
title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia",
doi = "10.1007/s10646-023-02663-6"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić-Jeftić, Ž., Rašković, B., Poleksić, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology.
https://doi.org/10.1007/s10646-023-02663-6

Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić-Jeftić Ž, Rašković B, Poleksić V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology. 2023;.
doi:10.1007/s10646-023-02663-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić-Jeftić, Željka, Rašković, Božidar, Poleksić, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Ecotoxicology (2023),
https://doi.org/10.1007/s10646-023-02663-6 . .

TY  - JOUR
AU  - Cvetković, Stefana
AU  - Tenji, Dina
AU  - Mitić-Ćulafić, Dragana
AU  - Vuletić, Stefana
AU  - Ganić, Tea
AU  - Djekić, Ilija
AU  - Nikolić, Biljana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6377
AB  - As a foodborne pathogen, Listeria monocytogenes could be commonly found in the food processing facilities, where forms biofilm on various food contact surfaces. Therefore, the control of L. monocytogenes biofilm formation is of key importance for improving food safety. The aim of this work was to examine the ability of yellow gentian root and leaf extracts to inhibit biofilm formation of L. monocytogenes strains at commonly used 37 °C and at ambient temperature associated with food processing plants (18 °C), on both polystyrene and stainless steel. Crystal violet staining assay revealed strong potential of the extracts to prevent biofilm formation on polystyrene at 37 °C (up to 90% inhibition), and on both surfaces at 18 °C (up to 78.1% and 62.9% on polystyrene and stainless steel, respectively). Antibiofilm effect could be ascribed to extracts’ ability to reduce the production of exopolysaccharides and to suppress swimming motility. Finally, qRT-PCR demonstrated no activity against quorum sensing, but up-regulation of the genes (flaA, sigB) involved in biofilm formation, most likely as a response to stressful conditions. Results obtained suggest yellow gentian extracts as potent antibiofilm agents, but further analyses are needed to determine the underlie mechanism and to validate the efficiency and safety in commercial environment. © 2023 Elsevier Ltd
T2  - Food Bioscience
T2  - Food Bioscience
T1  - Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces
VL  - 54
DO  - 10.1016/j.fbio.2023.102857
ER  - 

@article{
author = "Cvetković, Stefana and Tenji, Dina and Mitić-Ćulafić, Dragana and Vuletić, Stefana and Ganić, Tea and Djekić, Ilija and Nikolić, Biljana",
year = "2023",
abstract = "As a foodborne pathogen, Listeria monocytogenes could be commonly found in the food processing facilities, where forms biofilm on various food contact surfaces. Therefore, the control of L. monocytogenes biofilm formation is of key importance for improving food safety. The aim of this work was to examine the ability of yellow gentian root and leaf extracts to inhibit biofilm formation of L. monocytogenes strains at commonly used 37 °C and at ambient temperature associated with food processing plants (18 °C), on both polystyrene and stainless steel. Crystal violet staining assay revealed strong potential of the extracts to prevent biofilm formation on polystyrene at 37 °C (up to 90% inhibition), and on both surfaces at 18 °C (up to 78.1% and 62.9% on polystyrene and stainless steel, respectively). Antibiofilm effect could be ascribed to extracts’ ability to reduce the production of exopolysaccharides and to suppress swimming motility. Finally, qRT-PCR demonstrated no activity against quorum sensing, but up-regulation of the genes (flaA, sigB) involved in biofilm formation, most likely as a response to stressful conditions. Results obtained suggest yellow gentian extracts as potent antibiofilm agents, but further analyses are needed to determine the underlie mechanism and to validate the efficiency and safety in commercial environment. © 2023 Elsevier Ltd",
journal = "Food Bioscience, Food Bioscience",
title = "Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces",
volume = "54",
doi = "10.1016/j.fbio.2023.102857"
}

Cvetković, S., Tenji, D., Mitić-Ćulafić, D., Vuletić, S., Ganić, T., Djekić, I.,& Nikolić, B.. (2023). Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces. in Food Bioscience, 54.
https://doi.org/10.1016/j.fbio.2023.102857

Cvetković S, Tenji D, Mitić-Ćulafić D, Vuletić S, Ganić T, Djekić I, Nikolić B. Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces. in Food Bioscience. 2023;54.
doi:10.1016/j.fbio.2023.102857 .

Cvetković, Stefana, Tenji, Dina, Mitić-Ćulafić, Dragana, Vuletić, Stefana, Ganić, Tea, Djekić, Ilija, Nikolić, Biljana, "Potential of yellow gentian aqueous-ethanolic extracts to prevent Listeria monocytogenes biofilm formation on selected food contact surfaces" in Food Bioscience, 54 (2023),
https://doi.org/10.1016/j.fbio.2023.102857 . .

TY  - JOUR
AU  - Bogdanović, Svetlana
AU  - Stanković, Slaviša
AU  - Berić, Tanja
AU  - Tomasevic, Igor
AU  - Heinz, Volker
AU  - Terjung, Nino
AU  - Dimkić, Ivica
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6288
AB  - “Pirot ‘ironed’ sausage“ (Pis) is a traditional, fermented sausage, made from different types of meat (beef and chevon), without additives or starter cultures. The physical–chemical properties (pH, water activity, fats, moisture, and protein contents) were examined in the initial meat batter stuffing and during ripening. Total bacterial diversity was examined at different time points using both culturable (traditional) and non-culturable (NGS sequencing) approaches. During the ripening, a decrease in pH value, aw, and moisture content was observed, as well as an increase in protein and fat content. At least a two-fold significant decrease was noted for colorimetric values during the ripening period. The dominance of Proteobacteria and Firmicutes was observed in the non-culturable approach in all studied samples. During the ripening process, an increase in Firmicutes (from 33.5% to 63.5%) with a decrease in Proteobacteria (from 65.4% to 22.3%) was observed. The bacterial genera that were dominant throughout the ripening process were Lactobacillus, Photobacterium, Leuconostoc, Weissella, and Lactococcus, while Carnobacterium, Brochothrix, and Acinetobacter were found also, but in negligible abundance. Among the culturable bacteria, Latilactobacillus sakei (Lactobacillus sakei) and Leuconostoc mesenteoides were present in all stages of ripening. © 2023 by the authors.
T2  - Foods
T2  - Foods
T1  - Bacteriobiota and Chemical Changes during the Ripening of
Traditional Fermented “Pirot ‘Ironed’ Sausage”
IS  - 3
VL  - 12
DO  - 10.3390/foods12030664
ER  - 

@article{
author = "Bogdanović, Svetlana and Stanković, Slaviša and Berić, Tanja and Tomasevic, Igor and Heinz, Volker and Terjung, Nino and Dimkić, Ivica",
year = "2023",
abstract = "“Pirot ‘ironed’ sausage“ (Pis) is a traditional, fermented sausage, made from different types of meat (beef and chevon), without additives or starter cultures. The physical–chemical properties (pH, water activity, fats, moisture, and protein contents) were examined in the initial meat batter stuffing and during ripening. Total bacterial diversity was examined at different time points using both culturable (traditional) and non-culturable (NGS sequencing) approaches. During the ripening, a decrease in pH value, aw, and moisture content was observed, as well as an increase in protein and fat content. At least a two-fold significant decrease was noted for colorimetric values during the ripening period. The dominance of Proteobacteria and Firmicutes was observed in the non-culturable approach in all studied samples. During the ripening process, an increase in Firmicutes (from 33.5% to 63.5%) with a decrease in Proteobacteria (from 65.4% to 22.3%) was observed. The bacterial genera that were dominant throughout the ripening process were Lactobacillus, Photobacterium, Leuconostoc, Weissella, and Lactococcus, while Carnobacterium, Brochothrix, and Acinetobacter were found also, but in negligible abundance. Among the culturable bacteria, Latilactobacillus sakei (Lactobacillus sakei) and Leuconostoc mesenteoides were present in all stages of ripening. © 2023 by the authors.",
journal = "Foods, Foods",
title = "Bacteriobiota and Chemical Changes during the Ripening of
Traditional Fermented “Pirot ‘Ironed’ Sausage”",
number = "3",
volume = "12",
doi = "10.3390/foods12030664"
}

Bogdanović, S., Stanković, S., Berić, T., Tomasevic, I., Heinz, V., Terjung, N.,& Dimkić, I.. (2023). Bacteriobiota and Chemical Changes during the Ripening of
Traditional Fermented “Pirot ‘Ironed’ Sausage”. in Foods, 12(3).
https://doi.org/10.3390/foods12030664

Bogdanović S, Stanković S, Berić T, Tomasevic I, Heinz V, Terjung N, Dimkić I. Bacteriobiota and Chemical Changes during the Ripening of
Traditional Fermented “Pirot ‘Ironed’ Sausage”. in Foods. 2023;12(3).
doi:10.3390/foods12030664 .

Bogdanović, Svetlana, Stanković, Slaviša, Berić, Tanja, Tomasevic, Igor, Heinz, Volker, Terjung, Nino, Dimkić, Ivica, "Bacteriobiota and Chemical Changes during the Ripening of
Traditional Fermented “Pirot ‘Ironed’ Sausage”" in Foods, 12, no. 3 (2023),
https://doi.org/10.3390/foods12030664 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Zečević, Katarina
AU  - Stanisavljević, Rade
AU  - Popović, Tatjana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6207
AB  - The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy. © 2022 British Society for Plant Pathology.
T2  - Plant Pathology
T2  - Plant Pathology
T1  - Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans
DO  - 10.1111/ppa.13658
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Milovanović, Predrag and Stanković, Slaviša and Zečević, Katarina and Stanisavljević, Rade and Popović, Tatjana",
year = "2022",
abstract = "The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy. © 2022 British Society for Plant Pathology.",
journal = "Plant Pathology, Plant Pathology",
title = "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans",
doi = "10.1111/ppa.13658"
}

Iličić, R., Jelušić, A., Milovanović, P., Stanković, S., Zečević, K., Stanisavljević, R.,& Popović, T.. (2022). Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in Plant Pathology.
https://doi.org/10.1111/ppa.13658

Iličić R, Jelušić A, Milovanović P, Stanković S, Zečević K, Stanisavljević R, Popović T. Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in Plant Pathology. 2022;.
doi:10.1111/ppa.13658 .

Iličić, Renata, Jelušić, Aleksandra, Milovanović, Predrag, Stanković, Slaviša, Zečević, Katarina, Stanisavljević, Rade, Popović, Tatjana, "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans" in Plant Pathology (2022),
https://doi.org/10.1111/ppa.13658 . .

TY  - CONF
AU  - Stojićević, Aleksandra
AU  - Alimpić, Ana
AU  - Pantić, Milena
AU  - Antić, Mališa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6628
AB  - Basil (Ocimum basilicum L.) has a long traditional use as spice in both folk and conventional medicine. Basil extracts (BEs) as well as essential oil (EO) represents a rich source of phytochemicals, many of which possess diverse biological activities. The objectives of this study were to assess antioxidant and antibacterial activities of BEs and EO, obtained from plants cultivated in Republic of Serbia. EO was isolated by hydrodistillation, while BEs were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. In prepared BEs total phenolic (TPC) and total flavonoid content (TFC) were determined spectrophotometrically. Antioxidant activity was evaluated by DPPH and FRAP assays. A higher TPC was obtained using 70% ethanol, while 96% ethanol proved to be more effective for the extraction of flavonoids. In both antioxidant assays, BEs showed remarkable antioxidant properties compared to EO. Also, regardless the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. Both antioxidant assays strongly correlated with TPC than with TFC. For antibacterial activity assay, minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. Typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes). Opposite to results of antioxidant activity, EO expressed superior antibacterial activity. Based on the obtained results, it can be concluded that basil represents powerful source of biologically active components which can express strong antioxidant and antibacterial activity.
C3  - 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland
T1  - Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts
DO  - 10.3390/ECMC2022-13482
ER  - 

@conference{
author = "Stojićević, Aleksandra and Alimpić, Ana and Pantić, Milena and Antić, Mališa",
year = "2022",
abstract = "Basil (Ocimum basilicum L.) has a long traditional use as spice in both folk and conventional medicine. Basil extracts (BEs) as well as essential oil (EO) represents a rich source of phytochemicals, many of which possess diverse biological activities. The objectives of this study were to assess antioxidant and antibacterial activities of BEs and EO, obtained from plants cultivated in Republic of Serbia. EO was isolated by hydrodistillation, while BEs were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. In prepared BEs total phenolic (TPC) and total flavonoid content (TFC) were determined spectrophotometrically. Antioxidant activity was evaluated by DPPH and FRAP assays. A higher TPC was obtained using 70% ethanol, while 96% ethanol proved to be more effective for the extraction of flavonoids. In both antioxidant assays, BEs showed remarkable antioxidant properties compared to EO. Also, regardless the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. Both antioxidant assays strongly correlated with TPC than with TFC. For antibacterial activity assay, minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. Typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes). Opposite to results of antioxidant activity, EO expressed superior antibacterial activity. Based on the obtained results, it can be concluded that basil represents powerful source of biologically active components which can express strong antioxidant and antibacterial activity.",
journal = "8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland",
title = "Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts",
doi = "10.3390/ECMC2022-13482"
}

Stojićević, A., Alimpić, A., Pantić, M.,& Antić, M.. (2022). Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts. in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland.
https://doi.org/10.3390/ECMC2022-13482

Stojićević A, Alimpić A, Pantić M, Antić M. Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts. in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland. 2022;.
doi:10.3390/ECMC2022-13482 .

Stojićević, Aleksandra, Alimpić, Ana, Pantić, Milena, Antić, Mališa, "Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts" in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland (2022),
https://doi.org/10.3390/ECMC2022-13482 . .

TY  - JOUR
AU  - Hladnik, Matjaž
AU  - Unković, Nikola
AU  - Janakiev, Tamara
AU  - Grbić, Milica Ljaljević
AU  - Arbeiter, Alenka Baruca
AU  - Stanković, Slaviša
AU  - Janaćković, Peđa
AU  - Gavrilović, Milan
AU  - Rančić, Dragana
AU  - Bandelj, Dunja
AU  - Dimkić, Ivica
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6217
AB  - The olive tree is one of the most important agricultural plants, affected by several pests and diseases that cause a severe decline in health status leading to crop losses. Olive leaf spot disease caused by the fungus Venturia oleaginea can result in complete tree defoliation and consequently lower yield. The aim of the study was to obtain new knowledge related to plant–pathogen interaction, reveal mechanisms of plant defense against the pathogen, and characterize fungal phyllosphere communities on infected and symptomless leaves that could contribute to the development of new plant breeding strategies and identification of novel biocontrol agents. The highly susceptible olive variety “Istrska Belica”' was selected for a detailed evaluation. Microscopy analyses led to the observation of raphides in the mesophyll and parenchyma cells of infected leaves and gave new insight into the complex V. oleaginea pathogenesis. Culturable and total phyllosphere mycobiota, obtained via metabarcoding approach, highlighted Didymella, Aureobasidium, Cladosporium, and Alternaria species as overlapping between infected and symptomless leaves. Only Venturia and Erythrobasidium in infected and Cladosporium in symptomless samples with higher abundance showed statistically significant differences. Based on the ecological role of identified taxa, it can be suggested that Cladosporium species might have potential antagonistic effects on V. oleaginea. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Microbial Ecology
T2  - Microbial Ecology
T1  - An Insight into an Olive Scab on the “Istrska Belica” Variety: Host‐Pathogen Interactions and Phyllosphere Mycobiome
DO  - 10.1007/s00248-022-02131-4
ER  - 

@article{
author = "Hladnik, Matjaž and Unković, Nikola and Janakiev, Tamara and Grbić, Milica Ljaljević and Arbeiter, Alenka Baruca and Stanković, Slaviša and Janaćković, Peđa and Gavrilović, Milan and Rančić, Dragana and Bandelj, Dunja and Dimkić, Ivica",
year = "2022",
abstract = "The olive tree is one of the most important agricultural plants, affected by several pests and diseases that cause a severe decline in health status leading to crop losses. Olive leaf spot disease caused by the fungus Venturia oleaginea can result in complete tree defoliation and consequently lower yield. The aim of the study was to obtain new knowledge related to plant–pathogen interaction, reveal mechanisms of plant defense against the pathogen, and characterize fungal phyllosphere communities on infected and symptomless leaves that could contribute to the development of new plant breeding strategies and identification of novel biocontrol agents. The highly susceptible olive variety “Istrska Belica”' was selected for a detailed evaluation. Microscopy analyses led to the observation of raphides in the mesophyll and parenchyma cells of infected leaves and gave new insight into the complex V. oleaginea pathogenesis. Culturable and total phyllosphere mycobiota, obtained via metabarcoding approach, highlighted Didymella, Aureobasidium, Cladosporium, and Alternaria species as overlapping between infected and symptomless leaves. Only Venturia and Erythrobasidium in infected and Cladosporium in symptomless samples with higher abundance showed statistically significant differences. Based on the ecological role of identified taxa, it can be suggested that Cladosporium species might have potential antagonistic effects on V. oleaginea. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Microbial Ecology, Microbial Ecology",
title = "An Insight into an Olive Scab on the “Istrska Belica” Variety: Host‐Pathogen Interactions and Phyllosphere Mycobiome",
doi = "10.1007/s00248-022-02131-4"
}

Hladnik, M., Unković, N., Janakiev, T., Grbić, M. L., Arbeiter, A. B., Stanković, S., Janaćković, P., Gavrilović, M., Rančić, D., Bandelj, D.,& Dimkić, I.. (2022). An Insight into an Olive Scab on the “Istrska Belica” Variety: Host‐Pathogen Interactions and Phyllosphere Mycobiome. in Microbial Ecology.
https://doi.org/10.1007/s00248-022-02131-4

Hladnik M, Unković N, Janakiev T, Grbić ML, Arbeiter AB, Stanković S, Janaćković P, Gavrilović M, Rančić D, Bandelj D, Dimkić I. An Insight into an Olive Scab on the “Istrska Belica” Variety: Host‐Pathogen Interactions and Phyllosphere Mycobiome. in Microbial Ecology. 2022;.
doi:10.1007/s00248-022-02131-4 .

Hladnik, Matjaž, Unković, Nikola, Janakiev, Tamara, Grbić, Milica Ljaljević, Arbeiter, Alenka Baruca, Stanković, Slaviša, Janaćković, Peđa, Gavrilović, Milan, Rančić, Dragana, Bandelj, Dunja, Dimkić, Ivica, "An Insight into an Olive Scab on the “Istrska Belica” Variety: Host‐Pathogen Interactions and Phyllosphere Mycobiome" in Microbial Ecology (2022),
https://doi.org/10.1007/s00248-022-02131-4 . .

TY  - JOUR
AU  - Kabaš, Eva
AU  - Vukojičić, Snežana
AU  - Aćić, Svetlana
AU  - Lakušić, Dmitar
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6091
AB  - Despite the fact that dry grassland vegetation on ultramafics has been the object of continuous research in previous centuries, there remains a knowledge gap in regard to the initial stages of vegetation on ultramafic substrates. The Stipadominated dry grasslands of Serbia and Kosovo representing various initial phases in the overgrowth of ultramafic rocks were the object of the present study. A total of 213 relevés were made in different steppe-like Stipa species-dominated grasslands on ultramafics of Serbia including Kosovo, and were then analysed in the context of Balkan dry grassland vegetation of the Festuco-Brometea class. For cluster analyses, new relevés were analysed in order to characterize them floristically and sintaxonomically. For describing associations, we used the concept of “relative fidelity’’ allowing us to find the optimum occurrence of a species within a group of floristically similar communities. The threshold of the phi value was selected at 0.1 for new associations. Four new associations were distinguished: Stachyo scardicae-Stipetum tirsae ass. nov., Euphorbio glabriflorae-Stipetum pulcherrimae ass. nov., Alysso serbici-Stipetum pulcherrimae ass. nov. and Thymo striati-Stipetum mayerii ass. nov.. Relevés dominated by Stipa novakii were asigned to previously described Stipetum novaki Kabaš et D. Lakušić 2013. The first three new associations were assigned to Potentillion visianii, while the latter was assigned to the Centaureo kosaninii-Bromion fibrosi alliance of the Halacsyetalia sentneri order.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - Phytosociology of Stipa-dominated steppe-like vegetation on the ultramafics of the Central Balkans
EP  - 27
IS  - 1
SP  - 17
VL  - 46
DO  - 10.2298/BOTSERB2201017K
ER  - 

@article{
author = "Kabaš, Eva and Vukojičić, Snežana and Aćić, Svetlana and Lakušić, Dmitar",
year = "2022",
abstract = "Despite the fact that dry grassland vegetation on ultramafics has been the object of continuous research in previous centuries, there remains a knowledge gap in regard to the initial stages of vegetation on ultramafic substrates. The Stipadominated dry grasslands of Serbia and Kosovo representing various initial phases in the overgrowth of ultramafic rocks were the object of the present study. A total of 213 relevés were made in different steppe-like Stipa species-dominated grasslands on ultramafics of Serbia including Kosovo, and were then analysed in the context of Balkan dry grassland vegetation of the Festuco-Brometea class. For cluster analyses, new relevés were analysed in order to characterize them floristically and sintaxonomically. For describing associations, we used the concept of “relative fidelity’’ allowing us to find the optimum occurrence of a species within a group of floristically similar communities. The threshold of the phi value was selected at 0.1 for new associations. Four new associations were distinguished: Stachyo scardicae-Stipetum tirsae ass. nov., Euphorbio glabriflorae-Stipetum pulcherrimae ass. nov., Alysso serbici-Stipetum pulcherrimae ass. nov. and Thymo striati-Stipetum mayerii ass. nov.. Relevés dominated by Stipa novakii were asigned to previously described Stipetum novaki Kabaš et D. Lakušić 2013. The first three new associations were assigned to Potentillion visianii, while the latter was assigned to the Centaureo kosaninii-Bromion fibrosi alliance of the Halacsyetalia sentneri order.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "Phytosociology of Stipa-dominated steppe-like vegetation on the ultramafics of the Central Balkans",
pages = "27-17",
number = "1",
volume = "46",
doi = "10.2298/BOTSERB2201017K"
}

Kabaš, E., Vukojičić, S., Aćić, S.,& Lakušić, D.. (2022). Phytosociology of Stipa-dominated steppe-like vegetation on the ultramafics of the Central Balkans. in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 46(1), 17-27.
https://doi.org/10.2298/BOTSERB2201017K

Kabaš E, Vukojičić S, Aćić S, Lakušić D. Phytosociology of Stipa-dominated steppe-like vegetation on the ultramafics of the Central Balkans. in Botanica Serbica. 2022;46(1):17-27.
doi:10.2298/BOTSERB2201017K .

Kabaš, Eva, Vukojičić, Snežana, Aćić, Svetlana, Lakušić, Dmitar, "Phytosociology of Stipa-dominated steppe-like vegetation on the ultramafics of the Central Balkans" in Botanica Serbica, 46, no. 1 (2022):17-27,
https://doi.org/10.2298/BOTSERB2201017K . .

TY  - JOUR
AU  - Vranković, Jelena
AU  - Božanić, Milenka
AU  - Živić, Miroslav
AU  - Marković, Zoran
AU  - Marjanović, Stefan
AU  - Golubović, Vukosav
AU  - Živić, Ivana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6210
AB  - A 102-day feeding trial was conducted to evaluate the effects of four different fish feeding patterns on carp pond water quality and antioxidant biomarkers [superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR), Glutathione S-transferase (GST) and Thiol groups (SH)], protein content and biomass of the midge Chironomus plumosus. Farmed fish were fed two commercial diets: Soprofish 25/7 Standard (containing 25% protein and 7 % fat) and Soprofish 32/7 Profi Effect (containing 32 % protein and 7 % fat). These feeds were combined during a feeding trial in four different feeding patterns, designated as A, B, C and D. In feeding pattern A, the fish received Soprofish 25/7 Standard throughout the experiment and in feeding pattern D, Soprofish 32/7 Profi Effect. During feeding patterns B and C, a mixed feeding pattern was used, alternating between lower and higher protein diets. The study revealed no significant effects (P > 0.05) of the feeding regimens on water quality, so their effects on C. plumosus larvae can be evaluated independently. The activities of the chironomid enzymes CAT and GR showed a clear statistically significant dependence on the feeding pattern (P < 0.05), which increased with increasing protein content. On the other hand, the activity of SOD as well as larval biomass were noticeably affected by water quality, the former increasing with its deterioration and the latter with its improvement. The activities of GPx and GST were correlated with each other, but no statistically significant relationship was found with any of the measured abiotic factors (P > 0.05). Overall, our results suggest that long-term supplementation with a highly concentrated protein diet may have preventive effects against oxidative stress and support the use of C. plumosus as a model for assessing the effects of organic pollution on pond zoobenthic fauna. © 2022
T2  - Aquaculture Reports
T2  - Aquaculture Reports
T1  - Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns
VL  - 27
DO  - 10.1016/j.aqrep.2022.101387
ER  - 

@article{
author = "Vranković, Jelena and Božanić, Milenka and Živić, Miroslav and Marković, Zoran and Marjanović, Stefan and Golubović, Vukosav and Živić, Ivana",
year = "2022",
abstract = "A 102-day feeding trial was conducted to evaluate the effects of four different fish feeding patterns on carp pond water quality and antioxidant biomarkers [superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR), Glutathione S-transferase (GST) and Thiol groups (SH)], protein content and biomass of the midge Chironomus plumosus. Farmed fish were fed two commercial diets: Soprofish 25/7 Standard (containing 25% protein and 7 % fat) and Soprofish 32/7 Profi Effect (containing 32 % protein and 7 % fat). These feeds were combined during a feeding trial in four different feeding patterns, designated as A, B, C and D. In feeding pattern A, the fish received Soprofish 25/7 Standard throughout the experiment and in feeding pattern D, Soprofish 32/7 Profi Effect. During feeding patterns B and C, a mixed feeding pattern was used, alternating between lower and higher protein diets. The study revealed no significant effects (P > 0.05) of the feeding regimens on water quality, so their effects on C. plumosus larvae can be evaluated independently. The activities of the chironomid enzymes CAT and GR showed a clear statistically significant dependence on the feeding pattern (P < 0.05), which increased with increasing protein content. On the other hand, the activity of SOD as well as larval biomass were noticeably affected by water quality, the former increasing with its deterioration and the latter with its improvement. The activities of GPx and GST were correlated with each other, but no statistically significant relationship was found with any of the measured abiotic factors (P > 0.05). Overall, our results suggest that long-term supplementation with a highly concentrated protein diet may have preventive effects against oxidative stress and support the use of C. plumosus as a model for assessing the effects of organic pollution on pond zoobenthic fauna. © 2022",
journal = "Aquaculture Reports, Aquaculture Reports",
title = "Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns",
volume = "27",
doi = "10.1016/j.aqrep.2022.101387"
}

Vranković, J., Božanić, M., Živić, M., Marković, Z., Marjanović, S., Golubović, V.,& Živić, I.. (2022). Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns. in Aquaculture Reports, 27.
https://doi.org/10.1016/j.aqrep.2022.101387

Vranković J, Božanić M, Živić M, Marković Z, Marjanović S, Golubović V, Živić I. Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns. in Aquaculture Reports. 2022;27.
doi:10.1016/j.aqrep.2022.101387 .

Vranković, Jelena, Božanić, Milenka, Živić, Miroslav, Marković, Zoran, Marjanović, Stefan, Golubović, Vukosav, Živić, Ivana, "Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns" in Aquaculture Reports, 27 (2022),
https://doi.org/10.1016/j.aqrep.2022.101387 . .

TY  - JOUR
AU  - Kostić‑Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun‑Kolarević, Margareta
AU  - Višnjić‑Jeftić, Željka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković‑Gačić, Branka
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5903
AB  - This study was conducted on the Danube River locality Višnjica, exposed to the discharge of the largest wastewater collector in Serbia’s capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
SP  - 465
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 

@article{
author = "Kostić‑Vuković, Jovana and Kolarević, Stoimir and Kračun‑Kolarević, Margareta and Višnjić‑Jeftić, Željka and Rašković, Božidar and Poleksić, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vuković‑Gačić, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Višnjica, exposed to the discharge of the largest wastewater collector in Serbia’s capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
pages = "465",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}

Kostić‑Vuković, J., Kolarević, S., Kračun‑Kolarević, M., Višnjić‑Jeftić, Ž., Rašković, B., Poleksić, V., Gačić, Z., Lenhardt, M.,& Vuković‑Gačić, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer Science and Business Media Deutschland GmbH., 193(8), 465.
https://doi.org/10.1007/s10661-021-09232-6

Kostić‑Vuković J, Kolarević S, Kračun‑Kolarević M, Višnjić‑Jeftić Ž, Rašković B, Poleksić V, Gačić Z, Lenhardt M, Vuković‑Gačić B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8):465.
doi:10.1007/s10661-021-09232-6 .

Kostić‑Vuković, Jovana, Kolarević, Stoimir, Kračun‑Kolarević, Margareta, Višnjić‑Jeftić, Željka, Rašković, Božidar, Poleksić, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vuković‑Gačić, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021):465,
https://doi.org/10.1007/s10661-021-09232-6 . .

TY  - CONF
AU  - Stojićević, Aleksandra
AU  - Alimpić Aradski, Ana
AU  - Pantić, Milena
AU  - Pantelić, Nebojša
AU  - Rabrenović, Biljana
AU  - Duletić-Laušević, Sonja
AU  - Antić, Mališa
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6630
AB  - Medicinal plants represent a rich source of phytochemicals, many of which possess diverse biological activities, including antioxidants. The objectives of this study were to assess antioxidant, antibacterial and anticancer activities of savory (Satureja montana L.) ethanolic extracts and to monitor the changes of oxidative status during long-term storage conditions of the cold pressed sunflower oil (CPSU) with the addition of extracts at various concentrations.  Extracts were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. Antioxidant activity was evaluated by DPPH and ꞵ-carotene bleaching assays. In both assays, extracts showed remarkable antioxidative properties which were equal or stronger than those of BHT and BHA. Also, regardless of the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. For antibacterial activity assay, minimal inhibitory concentrations (MIC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes) with MIC=1,25–10 μg/mL and 0,0781–0,3125 μg/mL, respectively. As results obtained by MTT test, the extracts didn’t show anticancer activity (IC50>100 μg/L) against tested tumor cell lines: PC-3 (prostate), HT-29 (colon) and HeLa (cervix) in the applied concentration range. For investigation of oxidative stability of CPSU, only 70% ethanol extracts were used. Samples of CPSU (with addition of 250, 500 and 1000 ppm of extracts), as well as control samples (with addition of 200 ppm of BHT and CPSU without any additives), were stored at room temperature for six months. In order to determine the level of oxidative changes, samples were analyzed for peroxide, anisidine value and conjugated dienes and trienes. Based on obtained results, it can be concluded that savory extracts are powerful natural antioxidants that can delay the oxidative deterioration of CPSU and whose effectiveness is comparable to the synthetic antioxidant BHT.
PB  - University of Belgrade
C3  - UniFood Conference
T1  - Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions
SP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6630
ER  - 

@conference{
author = "Stojićević, Aleksandra and Alimpić Aradski, Ana and Pantić, Milena and Pantelić, Nebojša and Rabrenović, Biljana and Duletić-Laušević, Sonja and Antić, Mališa and Nikšić, Miomir",
year = "2021",
abstract = "Medicinal plants represent a rich source of phytochemicals, many of which possess diverse biological activities, including antioxidants. The objectives of this study were to assess antioxidant, antibacterial and anticancer activities of savory (Satureja montana L.) ethanolic extracts and to monitor the changes of oxidative status during long-term storage conditions of the cold pressed sunflower oil (CPSU) with the addition of extracts at various concentrations.  Extracts were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. Antioxidant activity was evaluated by DPPH and ꞵ-carotene bleaching assays. In both assays, extracts showed remarkable antioxidative properties which were equal or stronger than those of BHT and BHA. Also, regardless of the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. For antibacterial activity assay, minimal inhibitory concentrations (MIC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes) with MIC=1,25–10 μg/mL and 0,0781–0,3125 μg/mL, respectively. As results obtained by MTT test, the extracts didn’t show anticancer activity (IC50>100 μg/L) against tested tumor cell lines: PC-3 (prostate), HT-29 (colon) and HeLa (cervix) in the applied concentration range. For investigation of oxidative stability of CPSU, only 70% ethanol extracts were used. Samples of CPSU (with addition of 250, 500 and 1000 ppm of extracts), as well as control samples (with addition of 200 ppm of BHT and CPSU without any additives), were stored at room temperature for six months. In order to determine the level of oxidative changes, samples were analyzed for peroxide, anisidine value and conjugated dienes and trienes. Based on obtained results, it can be concluded that savory extracts are powerful natural antioxidants that can delay the oxidative deterioration of CPSU and whose effectiveness is comparable to the synthetic antioxidant BHT.",
publisher = "University of Belgrade",
journal = "UniFood Conference",
title = "Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions",
pages = "101",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6630"
}

Stojićević, A., Alimpić Aradski, A., Pantić, M., Pantelić, N., Rabrenović, B., Duletić-Laušević, S., Antić, M.,& Nikšić, M.. (2021). Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions. in UniFood Conference
University of Belgrade., 101.
https://hdl.handle.net/21.15107/rcub_agrospace_6630

Stojićević A, Alimpić Aradski A, Pantić M, Pantelić N, Rabrenović B, Duletić-Laušević S, Antić M, Nikšić M. Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions. in UniFood Conference. 2021;:101.
https://hdl.handle.net/21.15107/rcub_agrospace_6630 .

Stojićević, Aleksandra, Alimpić Aradski, Ana, Pantić, Milena, Pantelić, Nebojša, Rabrenović, Biljana, Duletić-Laušević, Sonja, Antić, Mališa, Nikšić, Miomir, "Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions" in UniFood Conference (2021):101,
https://hdl.handle.net/21.15107/rcub_agrospace_6630 .

TY  - JOUR
AU  - Janaćković, Pedja
AU  - Gavrilović, Milan
AU  - Rančić, Dragana
AU  - Stešević, Danijela
AU  - Dajić-Stevanović, Zora
AU  - Marin, Petar D.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5848
AB  - In this study, the vegetative anatomy of Artemisia umbelliformis subsp. eriantha, a Central European alpine glacial relict, wild-growing on Mt. Durmitor (Mon- tenegro) was examined for the first time. The aim was to investigate the general anatomy and particular anatomical traits which might have possible taxonomic value. Microscopic slides were prepared according to the standard histological procedures. The adventitious young root showed a primary structure, while the older root showed a secondary structure with a well-developed periderm on its surface. The rhizome showed a primary structure with elements of a secondary structure (periderm). The stem cross section is characterized by a round shape with a well-developed periderm at certain stages, and collateral vascular bundles ar- ranged in a circle. The petiole is concave in shape with a single-layered epidermis and parenchyma tissue with one large and two small vascular bundles. Druses and rhombohedral crystals are observed inside some petiole parenchyma cells. The leaf lobe cross section has an oblong-linear shape and is isolateral and amphistomatous in structure. The anticlinal walls of the leaf epidermal cells are sinuate. Secretory canals are present in the root cortex parenchyma (endodermal secretory canals) and the rhizome cortical parenchyma. The stem, petiole and leaf lack secretory canals. The stem and leaf are covered with T-shaped non-glandular and glandular trichomes. The taxonomic value of the analyzed characteristics is briefly discussed.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - Anatomical traits of Artemisia umbelliformis subsp. eriantha (Asteraceae) alpine glacial relict from Mt. Durmitor (Montenegro)
EP  - 30
IS  - 1
SP  - 23
VL  - 45
DO  - 10.2298/BOTSERB2101023J
ER  - 

@article{
author = "Janaćković, Pedja and Gavrilović, Milan and Rančić, Dragana and Stešević, Danijela and Dajić-Stevanović, Zora and Marin, Petar D.",
year = "2021",
abstract = "In this study, the vegetative anatomy of Artemisia umbelliformis subsp. eriantha, a Central European alpine glacial relict, wild-growing on Mt. Durmitor (Mon- tenegro) was examined for the first time. The aim was to investigate the general anatomy and particular anatomical traits which might have possible taxonomic value. Microscopic slides were prepared according to the standard histological procedures. The adventitious young root showed a primary structure, while the older root showed a secondary structure with a well-developed periderm on its surface. The rhizome showed a primary structure with elements of a secondary structure (periderm). The stem cross section is characterized by a round shape with a well-developed periderm at certain stages, and collateral vascular bundles ar- ranged in a circle. The petiole is concave in shape with a single-layered epidermis and parenchyma tissue with one large and two small vascular bundles. Druses and rhombohedral crystals are observed inside some petiole parenchyma cells. The leaf lobe cross section has an oblong-linear shape and is isolateral and amphistomatous in structure. The anticlinal walls of the leaf epidermal cells are sinuate. Secretory canals are present in the root cortex parenchyma (endodermal secretory canals) and the rhizome cortical parenchyma. The stem, petiole and leaf lack secretory canals. The stem and leaf are covered with T-shaped non-glandular and glandular trichomes. The taxonomic value of the analyzed characteristics is briefly discussed.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "Anatomical traits of Artemisia umbelliformis subsp. eriantha (Asteraceae) alpine glacial relict from Mt. Durmitor (Montenegro)",
pages = "30-23",
number = "1",
volume = "45",
doi = "10.2298/BOTSERB2101023J"
}

Janaćković, P., Gavrilović, M., Rančić, D., Stešević, D., Dajić-Stevanović, Z.,& Marin, P. D.. (2021). Anatomical traits of Artemisia umbelliformis subsp. eriantha (Asteraceae) alpine glacial relict from Mt. Durmitor (Montenegro). in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 45(1), 23-30.
https://doi.org/10.2298/BOTSERB2101023J

Janaćković P, Gavrilović M, Rančić D, Stešević D, Dajić-Stevanović Z, Marin PD. Anatomical traits of Artemisia umbelliformis subsp. eriantha (Asteraceae) alpine glacial relict from Mt. Durmitor (Montenegro). in Botanica Serbica. 2021;45(1):23-30.
doi:10.2298/BOTSERB2101023J .

Janaćković, Pedja, Gavrilović, Milan, Rančić, Dragana, Stešević, Danijela, Dajić-Stevanović, Zora, Marin, Petar D., "Anatomical traits of Artemisia umbelliformis subsp. eriantha (Asteraceae) alpine glacial relict from Mt. Durmitor (Montenegro)" in Botanica Serbica, 45, no. 1 (2021):23-30,
https://doi.org/10.2298/BOTSERB2101023J . .

TY  - JOUR
AU  - Pantelić, Nebojša Đ.
AU  - Božić, Bojan
AU  - Zmejkovski, Bojana B.
AU  - Banjac, Nebojša R.
AU  - Dojčinović, Biljana
AU  - Wessjohann, Ludger A.
AU  - Kaluđerović, Goran N.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5869
AB  - The synthesis of novel triphenyltin(IV) compounds, Ph3SnLn (n = 1–3), with oxaprozin (3-(4,5-diphenyloxazol-2-yl)propanoic acid), HL1, and the new propanoic acid derivatives 3-(4,5bis(4-methoxylphenyl)oxazol-2-yl)propanoic acid, HL2, and 3-(2,5-dioxo-4,4-diphenylimidazolidin1-yl)propanoic acid, HL3, has been performed. The ligands represent commercial drugs or their derivatives and the tin complexes have been characterized by standard analytical methods. The in vitro antiproliferative activity of both ligands and organotin(IV) compounds has been evaluated on the following tumour cell lines: human prostate cancer (PC-3), human colorectal adenocarcinoma (HT29), breast cancer (MCF-7), and hepatocellular cancer (HepG2), as well as on normal mouse embryonic fibroblast cells (NIH3T3) with the aid of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-12 diphenyltetrazolium bromide) and CV (crystal violet) assays. Contrary to the inactive ligand precursors, all organotin(IV) carboxylates showed very good activity with IC50 values ranging from 0.100 to 0.758 µM. According to the CV assay (IC50 = 0.218 ± 0.025 µM), complex Ph3SnL1 demonstrated the highest cytotoxicity against the caspase 3 deficient MCF-7 cell line. Inductively coupled plasma mass spectrometry (ICP-MS) analysis indicated a two-fold lower concentration of tin in MCF-7 cells in comparison to platinum. To investigate the mechanism of action of the compound Ph3SnL1 on MCF-7 cells, morphological, autophagy and cell cycle analysis, as well as the activation of caspase and ROS/RNS and NO production, has been performed. Results suggest that Ph3SnL1 induces caspase-independent apoptosis in MCF-7 cells.
PB  - MDPI AG
T2  - Molecules
T1  - In Vitro Evaluation of Antiproliferative Properties of Novel Organotin(IV) Carboxylate Compounds with Propanoic Acid Derivatives on a Panel of Human Cancer Cell Lines
IS  - 11
SP  - 3199
VL  - 26
DO  - 10.3390/molecules26113199
ER  - 

@article{
author = "Pantelić, Nebojša Đ. and Božić, Bojan and Zmejkovski, Bojana B. and Banjac, Nebojša R. and Dojčinović, Biljana and Wessjohann, Ludger A. and Kaluđerović, Goran N.",
year = "2021",
abstract = "The synthesis of novel triphenyltin(IV) compounds, Ph3SnLn (n = 1–3), with oxaprozin (3-(4,5-diphenyloxazol-2-yl)propanoic acid), HL1, and the new propanoic acid derivatives 3-(4,5bis(4-methoxylphenyl)oxazol-2-yl)propanoic acid, HL2, and 3-(2,5-dioxo-4,4-diphenylimidazolidin1-yl)propanoic acid, HL3, has been performed. The ligands represent commercial drugs or their derivatives and the tin complexes have been characterized by standard analytical methods. The in vitro antiproliferative activity of both ligands and organotin(IV) compounds has been evaluated on the following tumour cell lines: human prostate cancer (PC-3), human colorectal adenocarcinoma (HT29), breast cancer (MCF-7), and hepatocellular cancer (HepG2), as well as on normal mouse embryonic fibroblast cells (NIH3T3) with the aid of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-12 diphenyltetrazolium bromide) and CV (crystal violet) assays. Contrary to the inactive ligand precursors, all organotin(IV) carboxylates showed very good activity with IC50 values ranging from 0.100 to 0.758 µM. According to the CV assay (IC50 = 0.218 ± 0.025 µM), complex Ph3SnL1 demonstrated the highest cytotoxicity against the caspase 3 deficient MCF-7 cell line. Inductively coupled plasma mass spectrometry (ICP-MS) analysis indicated a two-fold lower concentration of tin in MCF-7 cells in comparison to platinum. To investigate the mechanism of action of the compound Ph3SnL1 on MCF-7 cells, morphological, autophagy and cell cycle analysis, as well as the activation of caspase and ROS/RNS and NO production, has been performed. Results suggest that Ph3SnL1 induces caspase-independent apoptosis in MCF-7 cells.",
publisher = "MDPI AG",
journal = "Molecules",
title = "In Vitro Evaluation of Antiproliferative Properties of Novel Organotin(IV) Carboxylate Compounds with Propanoic Acid Derivatives on a Panel of Human Cancer Cell Lines",
number = "11",
pages = "3199",
volume = "26",
doi = "10.3390/molecules26113199"
}

Pantelić, N. Đ., Božić, B., Zmejkovski, B. B., Banjac, N. R., Dojčinović, B., Wessjohann, L. A.,& Kaluđerović, G. N.. (2021). In Vitro Evaluation of Antiproliferative Properties of Novel Organotin(IV) Carboxylate Compounds with Propanoic Acid Derivatives on a Panel of Human Cancer Cell Lines. in Molecules
MDPI AG., 26(11), 3199.
https://doi.org/10.3390/molecules26113199

Pantelić NĐ, Božić B, Zmejkovski BB, Banjac NR, Dojčinović B, Wessjohann LA, Kaluđerović GN. In Vitro Evaluation of Antiproliferative Properties of Novel Organotin(IV) Carboxylate Compounds with Propanoic Acid Derivatives on a Panel of Human Cancer Cell Lines. in Molecules. 2021;26(11):3199.
doi:10.3390/molecules26113199 .

Pantelić, Nebojša Đ., Božić, Bojan, Zmejkovski, Bojana B., Banjac, Nebojša R., Dojčinović, Biljana, Wessjohann, Ludger A., Kaluđerović, Goran N., "In Vitro Evaluation of Antiproliferative Properties of Novel Organotin(IV) Carboxylate Compounds with Propanoic Acid Derivatives on a Panel of Human Cancer Cell Lines" in Molecules, 26, no. 11 (2021):3199,
https://doi.org/10.3390/molecules26113199 . .

TY  - JOUR
AU  - Kočović, Dušica M.
AU  - Bajuk-Bogdanović, Danica
AU  - Pećinar, Ilinka
AU  - Božić Nedeljković, Biljana
AU  - Daković, Marko
AU  - Andjus, Pavle R.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5949
AB  - The objective of the study was to describe cellular and molecular markers of radioprotection by anisomycin, focusing on the changes in rat brain tissue. Two-month-old Wistar rats were exposed to a 60Co radiation source at a dose of 6 Gy, with or without radioprotection with anisomycin (150 mg/kg) administered subcutaneously 30 min before or 3 or 6 h after irradiation. Survivors were analyzed 30 days after treatment. Astroglial and microglial responses were investigated based on the expression of glial markers assessed with immunohistochemistry, and quantitative changes in brain biomolecules were investigated by Raman microspectroscopy. In addition, blood plasma levels of pro-inflammatory (interleukin 6 and tumor necrosis factor α) and anti-inflammatory (interleukin 10) cytokines were assessed. We found that application of anisomycin either before or after irradiation significantly decreased the expression of the microglial marker Iba-1. We also found an increased intensity of Raman spectral bands related to nucleic acids, as well as an increased level of cytokines when anisomycin was applied after irradiation. This suggests that the radioprotective effects of anisomycin are by decreasing Iba-1 expression and stabilizing genetic material by increasing the level of nucleic acids.
PB  - Oxford University Press
T2  - Journal of Radiation Research
T1  - Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin
EP  - 803
IS  - 5
SP  - 793
VL  - 62
DO  - 10.1093/jrr/rrab045
ER  - 

@article{
author = "Kočović, Dušica M. and Bajuk-Bogdanović, Danica and Pećinar, Ilinka and Božić Nedeljković, Biljana and Daković, Marko and Andjus, Pavle R.",
year = "2021",
abstract = "The objective of the study was to describe cellular and molecular markers of radioprotection by anisomycin, focusing on the changes in rat brain tissue. Two-month-old Wistar rats were exposed to a 60Co radiation source at a dose of 6 Gy, with or without radioprotection with anisomycin (150 mg/kg) administered subcutaneously 30 min before or 3 or 6 h after irradiation. Survivors were analyzed 30 days after treatment. Astroglial and microglial responses were investigated based on the expression of glial markers assessed with immunohistochemistry, and quantitative changes in brain biomolecules were investigated by Raman microspectroscopy. In addition, blood plasma levels of pro-inflammatory (interleukin 6 and tumor necrosis factor α) and anti-inflammatory (interleukin 10) cytokines were assessed. We found that application of anisomycin either before or after irradiation significantly decreased the expression of the microglial marker Iba-1. We also found an increased intensity of Raman spectral bands related to nucleic acids, as well as an increased level of cytokines when anisomycin was applied after irradiation. This suggests that the radioprotective effects of anisomycin are by decreasing Iba-1 expression and stabilizing genetic material by increasing the level of nucleic acids.",
publisher = "Oxford University Press",
journal = "Journal of Radiation Research",
title = "Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin",
pages = "803-793",
number = "5",
volume = "62",
doi = "10.1093/jrr/rrab045"
}

Kočović, D. M., Bajuk-Bogdanović, D., Pećinar, I., Božić Nedeljković, B., Daković, M.,& Andjus, P. R.. (2021). Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin. in Journal of Radiation Research
Oxford University Press., 62(5), 793-803.
https://doi.org/10.1093/jrr/rrab045

Kočović DM, Bajuk-Bogdanović D, Pećinar I, Božić Nedeljković B, Daković M, Andjus PR. Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin. in Journal of Radiation Research. 2021;62(5):793-803.
doi:10.1093/jrr/rrab045 .

Kočović, Dušica M., Bajuk-Bogdanović, Danica, Pećinar, Ilinka, Božić Nedeljković, Biljana, Daković, Marko, Andjus, Pavle R., "Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin" in Journal of Radiation Research, 62, no. 5 (2021):793-803,
https://doi.org/10.1093/jrr/rrab045 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Janacković, Pedja
AU  - Kolasinac, Stefan M.
AU  - Dajić-Stevanović, Zora
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5763
AB  - Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry
IS  - 6
VL  - 17
DO  - 10.1002/cbdv.202000097
ER  - 

@article{
author = "Kostić, Aleksandar and Janacković, Pedja and Kolasinac, Stefan M. and Dajić-Stevanović, Zora",
year = "2020",
abstract = "Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry",
number = "6",
volume = "17",
doi = "10.1002/cbdv.202000097"
}

Kostić, A., Janacković, P., Kolasinac, S. M.,& Dajić-Stevanović, Z.. (2020). Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 17(6).
https://doi.org/10.1002/cbdv.202000097

Kostić A, Janacković P, Kolasinac SM, Dajić-Stevanović Z. Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity. 2020;17(6).
doi:10.1002/cbdv.202000097 .

Kostić, Aleksandar, Janacković, Pedja, Kolasinac, Stefan M., Dajić-Stevanović, Zora, "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry" in Chemistry & Biodiversity, 17, no. 6 (2020),
https://doi.org/10.1002/cbdv.202000097 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Janacković, Pedja
AU  - Kolasinac, Stefan M.
AU  - Dajić-Stevanović, Zora
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5399
AB  - Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry
IS  - 6
VL  - 17
DO  - 10.1002/cbdv.202000097
ER  - 

@article{
author = "Kostić, Aleksandar and Janacković, Pedja and Kolasinac, Stefan M. and Dajić-Stevanović, Zora",
year = "2020",
abstract = "Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry",
number = "6",
volume = "17",
doi = "10.1002/cbdv.202000097"
}

Kostić, A., Janacković, P., Kolasinac, S. M.,& Dajić-Stevanović, Z.. (2020). Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 17(6).
https://doi.org/10.1002/cbdv.202000097

Kostić A, Janacković P, Kolasinac SM, Dajić-Stevanović Z. Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity. 2020;17(6).
doi:10.1002/cbdv.202000097 .

Kostić, Aleksandar, Janacković, Pedja, Kolasinac, Stefan M., Dajić-Stevanović, Zora, "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry" in Chemistry & Biodiversity, 17, no. 6 (2020),
https://doi.org/10.1002/cbdv.202000097 . .