TY  - JOUR
AU  - Kostić‑Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun‑Kolarević, Margareta
AU  - Višnjić‑Jeftić, Željka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković‑Gačić, Branka
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5903
AB  - This study was conducted on the Danube River locality Višnjica, exposed to the discharge of the largest wastewater collector in Serbia’s capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
SP  - 465
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 

@article{
author = "Kostić‑Vuković, Jovana and Kolarević, Stoimir and Kračun‑Kolarević, Margareta and Višnjić‑Jeftić, Željka and Rašković, Božidar and Poleksić, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vuković‑Gačić, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Višnjica, exposed to the discharge of the largest wastewater collector in Serbia’s capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
pages = "465",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}

Kostić‑Vuković, J., Kolarević, S., Kračun‑Kolarević, M., Višnjić‑Jeftić, Ž., Rašković, B., Poleksić, V., Gačić, Z., Lenhardt, M.,& Vuković‑Gačić, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer Science and Business Media Deutschland GmbH., 193(8), 465.
https://doi.org/10.1007/s10661-021-09232-6

Kostić‑Vuković J, Kolarević S, Kračun‑Kolarević M, Višnjić‑Jeftić Ž, Rašković B, Poleksić V, Gačić Z, Lenhardt M, Vuković‑Gačić B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8):465.
doi:10.1007/s10661-021-09232-6 .

Kostić‑Vuković, Jovana, Kolarević, Stoimir, Kračun‑Kolarević, Margareta, Višnjić‑Jeftić, Željka, Rašković, Božidar, Poleksić, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vuković‑Gačić, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021):465,
https://doi.org/10.1007/s10661-021-09232-6 . .

TY  - JOUR
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Visnjić-Jeftić, Zeljka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4465
AB  - Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons
EP  - 1681
SP  - 1670
VL  - 601
DO  - 10.1016/j.scitotenv.2017.05.273
ER  - 

@article{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Paunović, Momir and Visnjić-Jeftić, Zeljka and Rašković, Božidar and Poleksić, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2017",
abstract = "Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons",
pages = "1681-1670",
volume = "601",
doi = "10.1016/j.scitotenv.2017.05.273"
}

Kostić, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Paunović, M., Visnjić-Jeftić, Z., Rašković, B., Poleksić, V., Lenhardt, M.,& Vuković-Gačić, B.. (2017). The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 601, 1670-1681.
https://doi.org/10.1016/j.scitotenv.2017.05.273

Kostić J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Paunović M, Visnjić-Jeftić Z, Rašković B, Poleksić V, Lenhardt M, Vuković-Gačić B. The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment. 2017;601:1670-1681.
doi:10.1016/j.scitotenv.2017.05.273 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Paunović, Momir, Visnjić-Jeftić, Zeljka, Rašković, Božidar, Poleksić, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons" in Science of the Total Environment, 601 (2017):1670-1681,
https://doi.org/10.1016/j.scitotenv.2017.05.273 . .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Visnjić-Jeftić, Zeljka
AU  - Skorić, Stefan
AU  - Gačić, Zoran
AU  - Djikanović, Vesna
AU  - Jarić, Ivan
AU  - Lenhardt, Mirjana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3690
AB  - The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.
PB  - Wiley, Hoboken
T2  - Environmental Toxicology
T1  - Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution
EP  - 1161
IS  - 10
SP  - 1153
VL  - 30
DO  - 10.1002/tox.21988
ER  - 

@article{
author = "Rašković, Božidar and Poleksić, Vesna and Visnjić-Jeftić, Zeljka and Skorić, Stefan and Gačić, Zoran and Djikanović, Vesna and Jarić, Ivan and Lenhardt, Mirjana",
year = "2015",
abstract = "The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.",
publisher = "Wiley, Hoboken",
journal = "Environmental Toxicology",
title = "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution",
pages = "1161-1153",
number = "10",
volume = "30",
doi = "10.1002/tox.21988"
}

Rašković, B., Poleksić, V., Visnjić-Jeftić, Z., Skorić, S., Gačić, Z., Djikanović, V., Jarić, I.,& Lenhardt, M.. (2015). Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology
Wiley, Hoboken., 30(10), 1153-1161.
https://doi.org/10.1002/tox.21988

Rašković B, Poleksić V, Visnjić-Jeftić Z, Skorić S, Gačić Z, Djikanović V, Jarić I, Lenhardt M. Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology. 2015;30(10):1153-1161.
doi:10.1002/tox.21988 .

Rašković, Božidar, Poleksić, Vesna, Visnjić-Jeftić, Zeljka, Skorić, Stefan, Gačić, Zoran, Djikanović, Vesna, Jarić, Ivan, Lenhardt, Mirjana, "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution" in Environmental Toxicology, 30, no. 10 (2015):1153-1161,
https://doi.org/10.1002/tox.21988 . .

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Poleksić, Vesna
AU  - Vuković-Gačić, Branka
AU  - Rašković, Božidar
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Jarić, Ivan
AU  - Gačić, Zoran
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3781
AB  - Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.
PB  - University of Ljubljana - Veterinary Faculty
T2  - Slovenian Veterinary Research
T1  - Integrated use of different fish related parameters to assess the status of water bodies
EP  - 13
IS  - 1
SP  - 5
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3781
ER  - 

@article{
author = "Lenhardt, Mirjana and Poleksić, Vesna and Vuković-Gačić, Branka and Rašković, Božidar and Sunjog, Karolina and Kolarević, Stoimir and Jarić, Ivan and Gačić, Zoran",
year = "2015",
abstract = "Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.",
publisher = "University of Ljubljana - Veterinary Faculty",
journal = "Slovenian Veterinary Research",
title = "Integrated use of different fish related parameters to assess the status of water bodies",
pages = "13-5",
number = "1",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3781"
}

Lenhardt, M., Poleksić, V., Vuković-Gačić, B., Rašković, B., Sunjog, K., Kolarević, S., Jarić, I.,& Gačić, Z.. (2015). Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research
University of Ljubljana - Veterinary Faculty., 52(1), 5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781

Lenhardt M, Poleksić V, Vuković-Gačić B, Rašković B, Sunjog K, Kolarević S, Jarić I, Gačić Z. Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research. 2015;52(1):5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .

Lenhardt, Mirjana, Poleksić, Vesna, Vuković-Gačić, Branka, Rašković, Božidar, Sunjog, Karolina, Kolarević, Stoimir, Jarić, Ivan, Gačić, Zoran, "Integrated use of different fish related parameters to assess the status of water bodies" in Slovenian Veterinary Research, 52, no. 1 (2015):5-13,
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .

TY  - JOUR
AU  - Skorić, Stefan
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3021
AB  - Cormorants cause damage to fish in aquaculture not only by consuming large number of fish but also by wounding them. Quantification of the actual damage in fish induced by wounding is important for reliable estimations of the overall loss in aquaculture caused by cormorants. In this study, specimens of mirror and scaly carp ( L.) injured by attacks of great cormorant ( L.) were collected from the "Eka" fish farm in Serbia. Surfaces of superficial and open subepithelial wounds (expressed as a percent of the total body surface), estimated by computer-assisted image analysis, had mean values 10.66 +/- A 1.86 and 0.85 +/- A 0.90, respectively. Histological evaluation of wounds was performed by two scoring systems: system proposed by Bernet et al. (J Fish Dis 22:25-34, 1999) and modified system described by Wahli et al. (Aquaculture 225:371-386, 2003). The most frequent histopathological changes were disturbed epidermal structure and surface cell morphology and increased number of club cells (on more than 75% of specimens); increased number of melanocytes and their distribution in different layers in dermis; and enlarged hypodermis (more than 80% of samples) and myolysis (50% of samples). Comparison of wounds' surfaces (surface of superficial injures) and histopathological changes, estimated by two scoring systems, revealed significant positive correlation. Bernet's scoring system showed higher correlation coefficients, when compared with the modified Wahli's scoring system.
PB  - Springer, Dordrecht
T2  - Aquaculture International
T1  - Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices
EP  - 535
IS  - 3
SP  - 525
VL  - 20
DO  - 10.1007/s10499-011-9483-3
ER  - 

@article{
author = "Skorić, Stefan and Rašković, Božidar and Poleksić, Vesna and Gačić, Zoran and Lenhardt, Mirjana",
year = "2012",
abstract = "Cormorants cause damage to fish in aquaculture not only by consuming large number of fish but also by wounding them. Quantification of the actual damage in fish induced by wounding is important for reliable estimations of the overall loss in aquaculture caused by cormorants. In this study, specimens of mirror and scaly carp ( L.) injured by attacks of great cormorant ( L.) were collected from the "Eka" fish farm in Serbia. Surfaces of superficial and open subepithelial wounds (expressed as a percent of the total body surface), estimated by computer-assisted image analysis, had mean values 10.66 +/- A 1.86 and 0.85 +/- A 0.90, respectively. Histological evaluation of wounds was performed by two scoring systems: system proposed by Bernet et al. (J Fish Dis 22:25-34, 1999) and modified system described by Wahli et al. (Aquaculture 225:371-386, 2003). The most frequent histopathological changes were disturbed epidermal structure and surface cell morphology and increased number of club cells (on more than 75% of specimens); increased number of melanocytes and their distribution in different layers in dermis; and enlarged hypodermis (more than 80% of samples) and myolysis (50% of samples). Comparison of wounds' surfaces (surface of superficial injures) and histopathological changes, estimated by two scoring systems, revealed significant positive correlation. Bernet's scoring system showed higher correlation coefficients, when compared with the modified Wahli's scoring system.",
publisher = "Springer, Dordrecht",
journal = "Aquaculture International",
title = "Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices",
pages = "535-525",
number = "3",
volume = "20",
doi = "10.1007/s10499-011-9483-3"
}

Skorić, S., Rašković, B., Poleksić, V., Gačić, Z.,& Lenhardt, M.. (2012). Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices. in Aquaculture International
Springer, Dordrecht., 20(3), 525-535.
https://doi.org/10.1007/s10499-011-9483-3

Skorić S, Rašković B, Poleksić V, Gačić Z, Lenhardt M. Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices. in Aquaculture International. 2012;20(3):525-535.
doi:10.1007/s10499-011-9483-3 .

Skorić, Stefan, Rašković, Božidar, Poleksić, Vesna, Gačić, Zoran, Lenhardt, Mirjana, "Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices" in Aquaculture International, 20, no. 3 (2012):525-535,
https://doi.org/10.1007/s10499-011-9483-3 . .

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Poleksić, Vesna
AU  - Višnjić-Jeftić, Z.
AU  - Kolarević, Stoimir
AU  - Jarić, Ivan
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2414
AB  - Anthropogenic influences on fish populations in river ecosystems can be studied at the individual level, as well as through the assessment of fish fauna composition. This study presents research that was focused at the individual level, such as the analysis of genotoxic effects (Comet assay), assessment of the heavy metal accumulation in fish liver, gills, intestine and muscle, histopathological analysis of liver, gills and skin, presence of parasites and the level of parasite infestation, hepatosomatic index and the condition factor. At the level of fish fauna composition, monitoring of the changes caused by dam construction was conducted through the use of modified index of biotic integrity (IBI). The aim of this work was to analyze all above listed parameters as potential bioindicators that can be utilized to assess the extent of disturbance of river systems in Serbia by anthropogenic impacts.
PB  - Vasile Goldis Western University, Arad
T2  - Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
T1  - Ecological status of Serbian rivers based on an ichthyological assessment
EP  - 860
IS  - 4
SP  - 855
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2414
ER  - 

@article{
author = "Lenhardt, Mirjana and Gačić, Zoran and Vuković-Gačić, Branka and Poleksić, Vesna and Višnjić-Jeftić, Z. and Kolarević, Stoimir and Jarić, Ivan",
year = "2011",
abstract = "Anthropogenic influences on fish populations in river ecosystems can be studied at the individual level, as well as through the assessment of fish fauna composition. This study presents research that was focused at the individual level, such as the analysis of genotoxic effects (Comet assay), assessment of the heavy metal accumulation in fish liver, gills, intestine and muscle, histopathological analysis of liver, gills and skin, presence of parasites and the level of parasite infestation, hepatosomatic index and the condition factor. At the level of fish fauna composition, monitoring of the changes caused by dam construction was conducted through the use of modified index of biotic integrity (IBI). The aim of this work was to analyze all above listed parameters as potential bioindicators that can be utilized to assess the extent of disturbance of river systems in Serbia by anthropogenic impacts.",
publisher = "Vasile Goldis Western University, Arad",
journal = "Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii",
title = "Ecological status of Serbian rivers based on an ichthyological assessment",
pages = "860-855",
number = "4",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2414"
}

Lenhardt, M., Gačić, Z., Vuković-Gačić, B., Poleksić, V., Višnjić-Jeftić, Z., Kolarević, S.,& Jarić, I.. (2011). Ecological status of Serbian rivers based on an ichthyological assessment. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
Vasile Goldis Western University, Arad., 21(4), 855-860.
https://hdl.handle.net/21.15107/rcub_agrospace_2414

Lenhardt M, Gačić Z, Vuković-Gačić B, Poleksić V, Višnjić-Jeftić Z, Kolarević S, Jarić I. Ecological status of Serbian rivers based on an ichthyological assessment. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii. 2011;21(4):855-860.
https://hdl.handle.net/21.15107/rcub_agrospace_2414 .

Lenhardt, Mirjana, Gačić, Zoran, Vuković-Gačić, Branka, Poleksić, Vesna, Višnjić-Jeftić, Z., Kolarević, Stoimir, Jarić, Ivan, "Ecological status of Serbian rivers based on an ichthyological assessment" in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii, 21, no. 4 (2011):855-860,
https://hdl.handle.net/21.15107/rcub_agrospace_2414 .

TY  - CONF
AU  - Poleksić, Vesna
AU  - Lenhardt, Mirjana
AU  - Jarić, Ivan
AU  - Djordjević, Dragana
AU  - Gačić, Zoran
AU  - Cvijanović, Gorcin
AU  - Rašković, Božidar
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2377
AB  - The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations. Environ. Toxicol. Chem. 2010; 29: 515-521.
PB  - Setac Press, Pensacola
C3  - Environmental Toxicology and Chemistry
T1  - Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)
EP  - 521
IS  - 3
SP  - 515
VL  - 29
DO  - 10.1002/etc.82
ER  - 

@conference{
author = "Poleksić, Vesna and Lenhardt, Mirjana and Jarić, Ivan and Djordjević, Dragana and Gačić, Zoran and Cvijanović, Gorcin and Rašković, Božidar",
year = "2010",
abstract = "The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations. Environ. Toxicol. Chem. 2010; 29: 515-521.",
publisher = "Setac Press, Pensacola",
journal = "Environmental Toxicology and Chemistry",
title = "Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)",
pages = "521-515",
number = "3",
volume = "29",
doi = "10.1002/etc.82"
}

Poleksić, V., Lenhardt, M., Jarić, I., Djordjević, D., Gačić, Z., Cvijanović, G.,& Rašković, B.. (2010). Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry
Setac Press, Pensacola., 29(3), 515-521.
https://doi.org/10.1002/etc.82

Poleksić V, Lenhardt M, Jarić I, Djordjević D, Gačić Z, Cvijanović G, Rašković B. Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry. 2010;29(3):515-521.
doi:10.1002/etc.82 .

Poleksić, Vesna, Lenhardt, Mirjana, Jarić, Ivan, Djordjević, Dragana, Gačić, Zoran, Cvijanović, Gorcin, Rašković, Božidar, "Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)" in Environmental Toxicology and Chemistry, 29, no. 3 (2010):515-521,
https://doi.org/10.1002/etc.82 . .