TY  - JOUR
AU  - Kočović, Dušica M.
AU  - Bajuk-Bogdanović, Danica
AU  - Pećinar, Ilinka
AU  - Božić Nedeljković, Biljana
AU  - Daković, Marko
AU  - Andjus, Pavle R.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5949
AB  - The objective of the study was to describe cellular and molecular markers of radioprotection by anisomycin, focusing on the changes in rat brain tissue. Two-month-old Wistar rats were exposed to a 60Co radiation source at a dose of 6 Gy, with or without radioprotection with anisomycin (150 mg/kg) administered subcutaneously 30 min before or 3 or 6 h after irradiation. Survivors were analyzed 30 days after treatment. Astroglial and microglial responses were investigated based on the expression of glial markers assessed with immunohistochemistry, and quantitative changes in brain biomolecules were investigated by Raman microspectroscopy. In addition, blood plasma levels of pro-inflammatory (interleukin 6 and tumor necrosis factor α) and anti-inflammatory (interleukin 10) cytokines were assessed. We found that application of anisomycin either before or after irradiation significantly decreased the expression of the microglial marker Iba-1. We also found an increased intensity of Raman spectral bands related to nucleic acids, as well as an increased level of cytokines when anisomycin was applied after irradiation. This suggests that the radioprotective effects of anisomycin are by decreasing Iba-1 expression and stabilizing genetic material by increasing the level of nucleic acids.
PB  - Oxford University Press
T2  - Journal of Radiation Research
T1  - Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin
EP  - 803
IS  - 5
SP  - 793
VL  - 62
DO  - 10.1093/jrr/rrab045
ER  - 

@article{
author = "Kočović, Dušica M. and Bajuk-Bogdanović, Danica and Pećinar, Ilinka and Božić Nedeljković, Biljana and Daković, Marko and Andjus, Pavle R.",
year = "2021",
abstract = "The objective of the study was to describe cellular and molecular markers of radioprotection by anisomycin, focusing on the changes in rat brain tissue. Two-month-old Wistar rats were exposed to a 60Co radiation source at a dose of 6 Gy, with or without radioprotection with anisomycin (150 mg/kg) administered subcutaneously 30 min before or 3 or 6 h after irradiation. Survivors were analyzed 30 days after treatment. Astroglial and microglial responses were investigated based on the expression of glial markers assessed with immunohistochemistry, and quantitative changes in brain biomolecules were investigated by Raman microspectroscopy. In addition, blood plasma levels of pro-inflammatory (interleukin 6 and tumor necrosis factor α) and anti-inflammatory (interleukin 10) cytokines were assessed. We found that application of anisomycin either before or after irradiation significantly decreased the expression of the microglial marker Iba-1. We also found an increased intensity of Raman spectral bands related to nucleic acids, as well as an increased level of cytokines when anisomycin was applied after irradiation. This suggests that the radioprotective effects of anisomycin are by decreasing Iba-1 expression and stabilizing genetic material by increasing the level of nucleic acids.",
publisher = "Oxford University Press",
journal = "Journal of Radiation Research",
title = "Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin",
pages = "803-793",
number = "5",
volume = "62",
doi = "10.1093/jrr/rrab045"
}

Kočović, D. M., Bajuk-Bogdanović, D., Pećinar, I., Božić Nedeljković, B., Daković, M.,& Andjus, P. R.. (2021). Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin. in Journal of Radiation Research
Oxford University Press., 62(5), 793-803.
https://doi.org/10.1093/jrr/rrab045

Kočović DM, Bajuk-Bogdanović D, Pećinar I, Božić Nedeljković B, Daković M, Andjus PR. Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin. in Journal of Radiation Research. 2021;62(5):793-803.
doi:10.1093/jrr/rrab045 .

Kočović, Dušica M., Bajuk-Bogdanović, Danica, Pećinar, Ilinka, Božić Nedeljković, Biljana, Daković, Marko, Andjus, Pavle R., "Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin" in Journal of Radiation Research, 62, no. 5 (2021):793-803,
https://doi.org/10.1093/jrr/rrab045 . .