TY  - JOUR
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Menković, Jelena
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6326
AB  - During May 2016, severe blight symptoms were observed in several
raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic
raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally,
eight raspberry strains, isolated earlier from two similar outbreaks, were
included in the study. Pathogenicity of the strains was confirmed on
detached raspberry and blackberry shoots by reproducing the symptoms
of natural infection. The strains were Gram-negative, fluorescent on
King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic,
arginine dihydrolase negative, and induced hypersensitivity in tobacco
leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all
strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability
tests suggested that isolated strains belong to Pseudomonas syringae.
The syrB gene associated with syringomycin production was detected
in all strains. DNA fingerprints with REP, ERIC, and BOX primers
generated identical profiles for 29 strains, except for strain KBI 222,
which showed a unique genomic fingerprint. In all, 9 of 10 selected
strains exhibited identical sequences of four housekeeping genes: gyrB,
rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in
strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree
based on a concatenated sequence of all four housekeeping genes,
strains clustered within phylogroup 2 (i.e., genomospecies 1) of the
P. syringae species complex, with pathotype strains of P. syringae pv.
aceris and P. syringae pv. solidagae as their closest relatives. There
was no correlation between genotype and geographic origin, particular
outbreak, host, or cultivar.
PB  - APS Publications
T2  - Plant Disease
T1  - Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia
EP  - 833
IS  - 107
SP  - 826
DO  - 10.1094/PDIS-06-22-1425-RE
ER  - 

@article{
author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2023",
abstract = "During May 2016, severe blight symptoms were observed in several
raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic
raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally,
eight raspberry strains, isolated earlier from two similar outbreaks, were
included in the study. Pathogenicity of the strains was confirmed on
detached raspberry and blackberry shoots by reproducing the symptoms
of natural infection. The strains were Gram-negative, fluorescent on
King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic,
arginine dihydrolase negative, and induced hypersensitivity in tobacco
leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all
strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability
tests suggested that isolated strains belong to Pseudomonas syringae.
The syrB gene associated with syringomycin production was detected
in all strains. DNA fingerprints with REP, ERIC, and BOX primers
generated identical profiles for 29 strains, except for strain KBI 222,
which showed a unique genomic fingerprint. In all, 9 of 10 selected
strains exhibited identical sequences of four housekeeping genes: gyrB,
rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in
strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree
based on a concatenated sequence of all four housekeeping genes,
strains clustered within phylogroup 2 (i.e., genomospecies 1) of the
P. syringae species complex, with pathotype strains of P. syringae pv.
aceris and P. syringae pv. solidagae as their closest relatives. There
was no correlation between genotype and geographic origin, particular
outbreak, host, or cultivar.",
publisher = "APS Publications",
journal = "Plant Disease",
title = "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia",
pages = "833-826",
number = "107",
doi = "10.1094/PDIS-06-22-1425-RE"
}

Ivanović, M., Prokić, A., Gašić, K., Menković, J., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2023). Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease
APS Publications.(107), 826-833.
https://doi.org/10.1094/PDIS-06-22-1425-RE

Ivanović M, Prokić A, Gašić K, Menković J, Kuzmanović N, Zlatković N, Obradović A. Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease. 2023;(107):826-833.
doi:10.1094/PDIS-06-22-1425-RE .

Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia" in Plant Disease, no. 107 (2023):826-833,
https://doi.org/10.1094/PDIS-06-22-1425-RE . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Živković, Svetlana
AU  - Obradović, Aleksa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6024
AB  - Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.
PB  - MDPI
T2  - Agronomy
T1  - Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia
IS  - 2
SP  - 235
VL  - 12
DO  - 10.3390/agronomy12020235
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Živković, Svetlana and Obradović, Aleksa",
year = "2022",
abstract = "Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.",
publisher = "MDPI",
journal = "Agronomy",
title = "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia",
number = "2",
pages = "235",
volume = "12",
doi = "10.3390/agronomy12020235"
}

Zlatković, N., Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Živković, S.,& Obradović, A.. (2022). Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy
MDPI., 12(2), 235.
https://doi.org/10.3390/agronomy12020235

Zlatković N, Gašić K, Kuzmanović N, Prokić A, Ivanović M, Živković S, Obradović A. Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy. 2022;12(2):235.
doi:10.3390/agronomy12020235 .

Zlatković, Nevena, Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Živković, Svetlana, Obradović, Aleksa, "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia" in Agronomy, 12, no. 2 (2022):235,
https://doi.org/10.3390/agronomy12020235 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6013
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
SP  - 803789
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Danijela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
pages = "803789",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gašić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, D.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media S.A.., 12, 803789.
https://doi.org/10.3389/fmicb.2021.803789

Gašić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić D, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12:803789.
doi:10.3389/fmicb.2021.803789 .

Gašić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Danijela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12 (2022):803789,
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - JOUR
AU  - Kurz, Mirjam
AU  - Carnal, Simon
AU  - Dafny-Yelin, Mery
AU  - Mairesse, Orly
AU  - Gottsberger, Richard A.
AU  - Ivanović, Milan
AU  - Grahovac, Mila
AU  - Lagonenko, Alexander L.
AU  - Drenova, Nataliya
AU  - Zharmukhamedova, Galiya
AU  - Doolotkeldieva, Tinatin
AU  - Smits, Theo H. M.
AU  - Rezzonico, Fabio
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5916
AB  - Fire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in the ensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogen outside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, however, not practical to sequence due to their size and variability. Here, we present a simple PCR assay targeting the duplication of a single CRISPR spacer in Erwinia amylovora that was found to be an important marker to discriminate between two main European populations of the pathogen. We implemented the assay on a total of 582 isolates to follow the spread of fire blight across the continent over several decades and, wherever possible, within single countries. The results obtained point to the occurrence of two major separate introduction events for E. amylovora in Europe that occurred approximately 20 years apart, and confirmed the existence of two principal distribution areas located in Northeastern Europe and the Eastern Mediterranean Basin from which the pathogen moved on to colonize the Eurasian continent.
PB  - BioMed Central Ltd
T2  - Phytopathology Research
T1  - Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer
IS  - 1
SP  - 18
VL  - 3
DO  - 10.1186/s42483-021-00096-9
ER  - 

@article{
author = "Kurz, Mirjam and Carnal, Simon and Dafny-Yelin, Mery and Mairesse, Orly and Gottsberger, Richard A. and Ivanović, Milan and Grahovac, Mila and Lagonenko, Alexander L. and Drenova, Nataliya and Zharmukhamedova, Galiya and Doolotkeldieva, Tinatin and Smits, Theo H. M. and Rezzonico, Fabio",
year = "2021",
abstract = "Fire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in the ensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogen outside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, however, not practical to sequence due to their size and variability. Here, we present a simple PCR assay targeting the duplication of a single CRISPR spacer in Erwinia amylovora that was found to be an important marker to discriminate between two main European populations of the pathogen. We implemented the assay on a total of 582 isolates to follow the spread of fire blight across the continent over several decades and, wherever possible, within single countries. The results obtained point to the occurrence of two major separate introduction events for E. amylovora in Europe that occurred approximately 20 years apart, and confirmed the existence of two principal distribution areas located in Northeastern Europe and the Eastern Mediterranean Basin from which the pathogen moved on to colonize the Eurasian continent.",
publisher = "BioMed Central Ltd",
journal = "Phytopathology Research",
title = "Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer",
number = "1",
pages = "18",
volume = "3",
doi = "10.1186/s42483-021-00096-9"
}

Kurz, M., Carnal, S., Dafny-Yelin, M., Mairesse, O., Gottsberger, R. A., Ivanović, M., Grahovac, M., Lagonenko, A. L., Drenova, N., Zharmukhamedova, G., Doolotkeldieva, T., Smits, T. H. M.,& Rezzonico, F.. (2021). Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. in Phytopathology Research
BioMed Central Ltd., 3(1), 18.
https://doi.org/10.1186/s42483-021-00096-9

Kurz M, Carnal S, Dafny-Yelin M, Mairesse O, Gottsberger RA, Ivanović M, Grahovac M, Lagonenko AL, Drenova N, Zharmukhamedova G, Doolotkeldieva T, Smits THM, Rezzonico F. Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. in Phytopathology Research. 2021;3(1):18.
doi:10.1186/s42483-021-00096-9 .

Kurz, Mirjam, Carnal, Simon, Dafny-Yelin, Mery, Mairesse, Orly, Gottsberger, Richard A., Ivanović, Milan, Grahovac, Mila, Lagonenko, Alexander L., Drenova, Nataliya, Zharmukhamedova, Galiya, Doolotkeldieva, Tinatin, Smits, Theo H. M., Rezzonico, Fabio, "Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer" in Phytopathology Research, 3, no. 1 (2021):18,
https://doi.org/10.1186/s42483-021-00096-9 . .

TY  - JOUR
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Pavlović, Z.
AU  - Obradović, Aleksa
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5379
AB  - Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia
EP  - 691
IS  - 3
SP  - 685
VL  - 157
DO  - 10.1007/s10658-020-02019-4
ER  - 

@article{
author = "Prokić, Andjelka and Zlatković, Nevena and Kuzmanović, Nemanja and Ivanović, Milan and Gašić, K. and Pavlović, Z. and Obradović, Aleksa",
year = "2020",
abstract = "Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia",
pages = "691-685",
number = "3",
volume = "157",
doi = "10.1007/s10658-020-02019-4"
}

Prokić, A., Zlatković, N., Kuzmanović, N., Ivanović, M., Gašić, K., Pavlović, Z.,& Obradović, A.. (2020). Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 157(3), 685-691.
https://doi.org/10.1007/s10658-020-02019-4

Prokić A, Zlatković N, Kuzmanović N, Ivanović M, Gašić K, Pavlović Z, Obradović A. Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology. 2020;157(3):685-691.
doi:10.1007/s10658-020-02019-4 .

Prokić, Andjelka, Zlatković, Nevena, Kuzmanović, Nemanja, Ivanović, Milan, Gašić, K., Pavlović, Z., Obradović, Aleksa, "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia" in European Journal of Plant Pathology, 157, no. 3 (2020):685-691,
https://doi.org/10.1007/s10658-020-02019-4 . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4912
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia
EP  - 2667
IS  - 10
SP  - 2667
VL  - 103
DO  - 10.1094/PDIS-12-18-2213-PDN
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Gašić, K. and Kuzmanović, Nemanja and Ivanović, Milan and Obradović, Aleksa",
year = "2019",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia",
pages = "2667-2667",
number = "10",
volume = "103",
doi = "10.1094/PDIS-12-18-2213-PDN"
}

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(10), 2667-2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667-2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Andjelka, Gašić, K., Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667-2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5009
AB  - Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material
EP  - 1052
IS  - 3
SP  - 1039
VL  - 51
DO  - 10.2298/GENSR1903039I
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Gašić, Katarina and Prokić, Andjelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2019",
abstract = "Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material",
pages = "1052-1039",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903039I"
}

Ivanović, M., Kuzmanović, N., Gašić, K., Prokić, A., Zlatković, N.,& Obradović, A.. (2019). Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika
Društvo genetičara Srbije, Beograd., 51(3), 1039-1052.
https://doi.org/10.2298/GENSR1903039I

Ivanović M, Kuzmanović N, Gašić K, Prokić A, Zlatković N, Obradović A. Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika. 2019;51(3):1039-1052.
doi:10.2298/GENSR1903039I .

Ivanović, Milan, Kuzmanović, Nemanja, Gašić, Katarina, Prokić, Andjelka, Zlatković, Nevena, Obradović, Aleksa, "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material" in Genetika, 51, no. 3 (2019):1039-1052,
https://doi.org/10.2298/GENSR1903039I . .

TY  - JOUR
AU  - Zivković, Sanja
AU  - Vasić, Tanja
AU  - Ivanović, Milan
AU  - Jevremović, Darko
AU  - Marković, Jordan
AU  - Trkulja, Vojislav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4958
AB  - During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Plant Diseases and Protection
T1  - Morphological and molecular identification of Eutypa lata on grapevine in Serbia
EP  - 483
IS  - 5
SP  - 479
VL  - 126
DO  - 10.1007/s41348-019-00238-4
ER  - 

@article{
author = "Zivković, Sanja and Vasić, Tanja and Ivanović, Milan and Jevremović, Darko and Marković, Jordan and Trkulja, Vojislav",
year = "2019",
abstract = "During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Plant Diseases and Protection",
title = "Morphological and molecular identification of Eutypa lata on grapevine in Serbia",
pages = "483-479",
number = "5",
volume = "126",
doi = "10.1007/s41348-019-00238-4"
}

Zivković, S., Vasić, T., Ivanović, M., Jevremović, D., Marković, J.,& Trkulja, V.. (2019). Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection
Springer Heidelberg, Heidelberg., 126(5), 479-483.
https://doi.org/10.1007/s41348-019-00238-4

Zivković S, Vasić T, Ivanović M, Jevremović D, Marković J, Trkulja V. Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection. 2019;126(5):479-483.
doi:10.1007/s41348-019-00238-4 .

Zivković, Sanja, Vasić, Tanja, Ivanović, Milan, Jevremović, Darko, Marković, Jordan, Trkulja, Vojislav, "Morphological and molecular identification of Eutypa lata on grapevine in Serbia" in Journal of Plant Diseases and Protection, 126, no. 5 (2019):479-483,
https://doi.org/10.1007/s41348-019-00238-4 . .

TY  - CONF
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4646
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot
EP  - 133
IS  - 10
SP  - 132
VL  - 108
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4646
ER  - 

@conference{
author = "Gašić, K. and Kuzmanović, Nemanja and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot",
pages = "133-132",
number = "10",
volume = "108",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4646"
}

Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N.,& Obradović, A.. (2018). Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 132-133.
https://hdl.handle.net/21.15107/rcub_agrospace_4646

Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Obradović A. Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology. 2018;108(10):132-133.
https://hdl.handle.net/21.15107/rcub_agrospace_4646 .

Gašić, K., Kuzmanović, Nemanja, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Obradović, Aleksa, "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot" in Phytopathology, 108, no. 10 (2018):132-133,
https://hdl.handle.net/21.15107/rcub_agrospace_4646 .

TY  - CONF
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4645
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro
EP  - 24
IS  - 10
SP  - 24
VL  - 108
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4645
ER  - 

@conference{
author = "Prokić, Andjelka and Ivanović, Milan and Gašić, K. and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro",
pages = "24-24",
number = "10",
volume = "108",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4645"
}

Prokić, A., Ivanović, M., Gašić, K., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2018). Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 24-24.
https://hdl.handle.net/21.15107/rcub_agrospace_4645

Prokić A, Ivanović M, Gašić K, Kuzmanović N, Zlatković N, Obradović A. Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology. 2018;108(10):24-24.
https://hdl.handle.net/21.15107/rcub_agrospace_4645 .

Prokić, Andjelka, Ivanović, Milan, Gašić, K., Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro" in Phytopathology, 108, no. 10 (2018):24-24,
https://hdl.handle.net/21.15107/rcub_agrospace_4645 .

TY  - JOUR
AU  - Živković, Sanja
AU  - Bulajić, Aleksandra
AU  - Vasić, Tanja
AU  - Ivanović, Milan
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4840
AB  - The eutypa dieback of grapevine (Vitis vinifera L.), caused by phytopathogenic fungus Eutypa lata, is one of the most severe diseases of trunk and other woody tissues of grapevine in the world. This disease, also known as the grapevine eutyposis, significantly reduces yield and quality of grapes, but primarily reduces the life span of the vines, causing partial or complete dying of grapevine. During 2004-2012, symptoms of dieback of grapevine were observed in Serbia and E. lata presence was confirmed by conventional and molecular identification methods. Symptoms first appear in the form of tiny, chlorotic and necrotic spots on the periphery of the leaf, followed by deformation of the leaves and the appearance of shortened shoots, often with the so-called zigzag growth of internodes. Subsequently, partial or complete dying of the grapevine occurs. The causal agent of eutypa dieback is E. lata - a vascular pathogen from the Diatrypaceae family. The infection occurs when ascospores reach fresh wounds formed by pruning, and in the presence of water droplets penetrate into the vascular tissue. Wounds are particularly sensitive immediately after pruning, although infections can occur seven weeks after the pruning. Eutyposis disease control requires integration of several different control measures. When pruning the grapevine, large scale wounding should be avoided. Pruned parts from diseased plants should be taken out of the vineyard and burned. If the symptoms are present, but the disease has not spread to the whole vine, the infected tissue should be pruned off together with at least 10 cm of healthy tissue. Fresh cuts should be preferably coated with fungicides such as thiophanate-methyl, pyraclostrobin or tebuconazole. In USA, paste with 5% (w/w) boric acid is successfully used for the coating of wounds. It is especially important to protect cuts during the restoration of grapevines or grafting grapevine on the two-year-old and the older grapevine, as well as in the large cuts inflicted in the main trunk. In the USA (California), it is recommended to conduct pruning as late as possible, just before bud swelling. Preventive measures include selection of less sensitive varieties and removal of potential disease hosts near the vineyard. Breeding forms of grapevine with lower stems and mixed pruning are also recommended.
AB  - Eutipozno odumiranje čokota vinove loze (Vitis vinifera L.), kojе prouzokuje fitopatogena gljiva Eutypa lata, predstavlja jednu od najštetnijih bolesti debla i ostalih drvenastih tkiva vinove loze u svetu. Ovо oboljenje, poznato i kao eutipoza vinove loze, značajno smanjuje prinos i kvalitet grožđa, ali pre svega skraćuje životni vek vinove loze, izazivajući delimično ili potpuno odumiranje čokota. U peroiodu od 2004 do 2012. godine, na teritoriji Srbije, uočeni su simptomi odumiranja čokota vinove loze, a prisustvo E. lata potvrđeno primenom konvencionalnih i molekularnih metoda. Simptomi se najpre ispoljavaju u vidu sitnih, hlorotičnih i nekrotičnih pega po obodu lista, praćeni deformacijom listova i pojavom skraćenih lastara, često sa takozvanim cik-cak porastom internodija. Vremenom dolazi do delimičnog ili potpunog odumiranja čokota vinove loze. Prouzrokovač eutipoznog odumiranja vinove loze je gljiva E. lata - vaskularni patogen iz familije Diatrypaceae. Infekcija nastaje kada askospore gljive dospeju na sveže preseke nastale rezidbom i u prisustvu vodenih kapi prodiru u vaskularno tkivo. Rane su posebno osetljive odmah nakon orezivanja, premda se infekcije mogu ostvariti i sedam nedelja nakon rezidbe. Mere zaštite od ove kompleksne bolesti zahtevaju integraciju više različitih mera borbe. Pri orezivanju vinove loze potrebno je izbegavati nanošenje velikih rana. Ukoliko se primete simptomi, ali se bolest nije proširila na ceo čokot, zaraženo tkivo treba odrezati zajedno sa najmanje 10 cm zdravog tkiva. Orezane delove sa obolelih biljaka bi trebalo izneti iz vinograda i spaliti. Sveže preseke poželjno je premazati fungicidima kao što su tiofanat - metil, piraklostrobin ili tebukonazol. Za premazivanje rana u SAD-a se uspešno koristi pasta sa 5% (w/w) bornom kiselinom. Posebno je važno zaštititi preseke pri obnavljaju čokota ili prekalemljivanju loze na dvogodišnjoj i starijoj lozi, kao i velike preseke na glavnom stablu. U SAD (Kaliforniji) preporučuje se što kasnija rezidba, ako je moguće pred samo bubrenje pupoljaka. Preventivne mere uključuju izbor manje osetljivih sorti i uklanjanje potencijalnih domaćina prouzrokovača u blizini vinograda. Za preventivu se preporučuje i izbor nekog od uzgojnih oblika koji podrazumevaju formiranje čokota sa niskim stablom - mešovita rezidba.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Eutypa dieback of grapevine
T1  - Eutipozno odumiranje čokota vinove loze
EP  - 658
IS  - 6
SP  - 647
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4840
ER  - 

@article{
author = "Živković, Sanja and Bulajić, Aleksandra and Vasić, Tanja and Ivanović, Milan",
year = "2018",
abstract = "The eutypa dieback of grapevine (Vitis vinifera L.), caused by phytopathogenic fungus Eutypa lata, is one of the most severe diseases of trunk and other woody tissues of grapevine in the world. This disease, also known as the grapevine eutyposis, significantly reduces yield and quality of grapes, but primarily reduces the life span of the vines, causing partial or complete dying of grapevine. During 2004-2012, symptoms of dieback of grapevine were observed in Serbia and E. lata presence was confirmed by conventional and molecular identification methods. Symptoms first appear in the form of tiny, chlorotic and necrotic spots on the periphery of the leaf, followed by deformation of the leaves and the appearance of shortened shoots, often with the so-called zigzag growth of internodes. Subsequently, partial or complete dying of the grapevine occurs. The causal agent of eutypa dieback is E. lata - a vascular pathogen from the Diatrypaceae family. The infection occurs when ascospores reach fresh wounds formed by pruning, and in the presence of water droplets penetrate into the vascular tissue. Wounds are particularly sensitive immediately after pruning, although infections can occur seven weeks after the pruning. Eutyposis disease control requires integration of several different control measures. When pruning the grapevine, large scale wounding should be avoided. Pruned parts from diseased plants should be taken out of the vineyard and burned. If the symptoms are present, but the disease has not spread to the whole vine, the infected tissue should be pruned off together with at least 10 cm of healthy tissue. Fresh cuts should be preferably coated with fungicides such as thiophanate-methyl, pyraclostrobin or tebuconazole. In USA, paste with 5% (w/w) boric acid is successfully used for the coating of wounds. It is especially important to protect cuts during the restoration of grapevines or grafting grapevine on the two-year-old and the older grapevine, as well as in the large cuts inflicted in the main trunk. In the USA (California), it is recommended to conduct pruning as late as possible, just before bud swelling. Preventive measures include selection of less sensitive varieties and removal of potential disease hosts near the vineyard. Breeding forms of grapevine with lower stems and mixed pruning are also recommended., Eutipozno odumiranje čokota vinove loze (Vitis vinifera L.), kojе prouzokuje fitopatogena gljiva Eutypa lata, predstavlja jednu od najštetnijih bolesti debla i ostalih drvenastih tkiva vinove loze u svetu. Ovо oboljenje, poznato i kao eutipoza vinove loze, značajno smanjuje prinos i kvalitet grožđa, ali pre svega skraćuje životni vek vinove loze, izazivajući delimično ili potpuno odumiranje čokota. U peroiodu od 2004 do 2012. godine, na teritoriji Srbije, uočeni su simptomi odumiranja čokota vinove loze, a prisustvo E. lata potvrđeno primenom konvencionalnih i molekularnih metoda. Simptomi se najpre ispoljavaju u vidu sitnih, hlorotičnih i nekrotičnih pega po obodu lista, praćeni deformacijom listova i pojavom skraćenih lastara, često sa takozvanim cik-cak porastom internodija. Vremenom dolazi do delimičnog ili potpunog odumiranja čokota vinove loze. Prouzrokovač eutipoznog odumiranja vinove loze je gljiva E. lata - vaskularni patogen iz familije Diatrypaceae. Infekcija nastaje kada askospore gljive dospeju na sveže preseke nastale rezidbom i u prisustvu vodenih kapi prodiru u vaskularno tkivo. Rane su posebno osetljive odmah nakon orezivanja, premda se infekcije mogu ostvariti i sedam nedelja nakon rezidbe. Mere zaštite od ove kompleksne bolesti zahtevaju integraciju više različitih mera borbe. Pri orezivanju vinove loze potrebno je izbegavati nanošenje velikih rana. Ukoliko se primete simptomi, ali se bolest nije proširila na ceo čokot, zaraženo tkivo treba odrezati zajedno sa najmanje 10 cm zdravog tkiva. Orezane delove sa obolelih biljaka bi trebalo izneti iz vinograda i spaliti. Sveže preseke poželjno je premazati fungicidima kao što su tiofanat - metil, piraklostrobin ili tebukonazol. Za premazivanje rana u SAD-a se uspešno koristi pasta sa 5% (w/w) bornom kiselinom. Posebno je važno zaštititi preseke pri obnavljaju čokota ili prekalemljivanju loze na dvogodišnjoj i starijoj lozi, kao i velike preseke na glavnom stablu. U SAD (Kaliforniji) preporučuje se što kasnija rezidba, ako je moguće pred samo bubrenje pupoljaka. Preventivne mere uključuju izbor manje osetljivih sorti i uklanjanje potencijalnih domaćina prouzrokovača u blizini vinograda. Za preventivu se preporučuje i izbor nekog od uzgojnih oblika koji podrazumevaju formiranje čokota sa niskim stablom - mešovita rezidba.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Eutypa dieback of grapevine, Eutipozno odumiranje čokota vinove loze",
pages = "658-647",
number = "6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4840"
}

Živković, S., Bulajić, A., Vasić, T.,& Ivanović, M.. (2018). Eutypa dieback of grapevine. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 46(6), 647-658.
https://hdl.handle.net/21.15107/rcub_agrospace_4840

Živković S, Bulajić A, Vasić T, Ivanović M. Eutypa dieback of grapevine. in Biljni lekar. 2018;46(6):647-658.
https://hdl.handle.net/21.15107/rcub_agrospace_4840 .

Živković, Sanja, Bulajić, Aleksandra, Vasić, Tanja, Ivanović, Milan, "Eutypa dieback of grapevine" in Biljni lekar, 46, no. 6 (2018):647-658,
https://hdl.handle.net/21.15107/rcub_agrospace_4840 .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4662
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 

@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Andjelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}

Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.02021

Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .

Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Andjelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .

TY  - JOUR
AU  - Krivokapić, Marija
AU  - Gavrilović, Veljko
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Fira, Djordje
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4901
AB  - The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation.
AB  - Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia
T1  - Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji
EP  - 184
IS  - 3-4
SP  - 175
VL  - 33
DO  - 10.2298/PIF1804175K
ER  - 

@article{
author = "Krivokapić, Marija and Gavrilović, Veljko and Ivanović, Milan and Kuzmanović, Nemanja and Fira, Djordje and Obradović, Aleksa and Gašić, Katarina",
year = "2018",
abstract = "The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation., Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia, Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji",
pages = "184-175",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804175K"
}

Krivokapić, M., Gavrilović, V., Ivanović, M., Kuzmanović, N., Fira, D., Obradović, A.,& Gašić, K.. (2018). Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(3-4), 175-184.
https://doi.org/10.2298/PIF1804175K

Krivokapić M, Gavrilović V, Ivanović M, Kuzmanović N, Fira D, Obradović A, Gašić K. Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina. 2018;33(3-4):175-184.
doi:10.2298/PIF1804175K .

Krivokapić, Marija, Gavrilović, Veljko, Ivanović, Milan, Kuzmanović, Nemanja, Fira, Djordje, Obradović, Aleksa, Gašić, Katarina, "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):175-184,
https://doi.org/10.2298/PIF1804175K . .

TY  - JOUR
AU  - Jerinić-Prodanović, Dušanka
AU  - Obradović, Aleksa
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Pavlović, Žaklina
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4551
AB  - Carrot psyllid Bactericera trigonica belongs to the family Triozidae, superfamily Psylloidea, order Hemiptera. Together with onion psyllid (B. tremblayi) and potato psyllid (B. nigricornis), it is a member of the complex Bactericera nigricornis Foerster with morphologically and bioecologically very similar species. It was originally described by Hodkinson (1981) as Trioza trigonica based on the analysis of adults collected from the carrots in Portugal, Italy, Cyprus, Turkey, Egypt and Iran. Later it has been transferred to the genus Bactericera together with the two other species from this complex. Adults and larvae B. trigonica, feed on carrot leaf and other Apiaceae. If they appears in large number, they can lead to chlorosis and leaf yellowing, which causes a yield reduction. Besides direct damage, its vector role of bacteria “Candidatus Liberibacter solanacearum” has been determined, which leads to root proliferation and weakening of plants from Apiaceae family due to chlorosis. Ca. Liberibacter solanacearum has been known in North Africa and New Zealand for a long time on plants from Solanacae family, potato in the first place, while in Europe it has been determined on carrot and cellery for the first time, with B. trigonica as its vector. The presence, biology and harmfulness of B. trigonica in the region of Serbia were studyed in the period from 2014 to 2017. It was found in 2014 for the first time and since then, its presence has been determined in all researched localities, during the whole vegetative period of carrot. Besides carrot, B. trigonica has been registered on celery, parsley and wild carrot as well. It has a several generations per year, overwintering as adults.
AB  - Mrkvina lisna buva Bactericera trigonica pripada familiji Triozidae, nadfamiliji Psylloidea, redu Hemiptera. Zajedno sa lukovom lisnom buvom (B. tremblayi) i krompirovom lisnom buvom (B. nigricornis) pripada kompleksu Bactericera nigricornis Foerster, u morfološkom i bioekološkom smislu veoma sličnih vrsta. Opisana je 1981. godine kao Trioza trigonica na osnovu analize imaga sakupljanih sa mrkve iz Portugalije, Italije, Kipra, Turske, Egipta i Irana. Kasnije je prebačena u rod Bactericera zajedno sa druge dve vrste ovog kompleksa. Imaga i larve se hrane na listu mrkve i drugih štitonoša. Ukoliko se jave u povećanoj brojnosti mogu dovesti do hloroze i žućenja lista, što ima za posledicu smanjenje prinosa. Pored direktnih šteta, utvrđena je njena vektorska uloga fastidiozne bakterije «Candidatus Liberibacter solanacearum», koja na biljkama iz familije Apiaceae dovodi do proliferacije korena i hlorotičnog slabljenja. Ca. L. solanacearum je odavno poznata u Severnoj Americi i Novom Zelandu na biljkama iz familije Solanacae, prevashodno na krompiru, a u Evropi je prvi put utvrđena na mrkvi i celeru, a B. trigonica kao njen vektor. U periodu od 2014. do 2017. godine proučavano je prisustvo, biologija i štetnost B. trigonica na području Srbije. Prvi put je registrovana 2014. godine i od tada je utvrđeno njeno prisustvo u svim lokalitetima istraživanja tokom celog vegetacionog perioda mrkve. Pored mrkve, B. trigonica je registrovana na celeru, peršunu i divljoj mrkvi. U toku godine ima veći broj generacija, prezimljava u stadijumu imaga na skrovitim mestima.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia
T1  - Rasprostranjenost, biologija i štetnost mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) u Srbiji
EP  - 384
IS  - 4
SP  - 375
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4551
ER  - 

@article{
author = "Jerinić-Prodanović, Dušanka and Obradović, Aleksa and Ivanović, Milan and Prokić, Andjelka and Zlatković, Nevena and Pavlović, Žaklina",
year = "2017",
abstract = "Carrot psyllid Bactericera trigonica belongs to the family Triozidae, superfamily Psylloidea, order Hemiptera. Together with onion psyllid (B. tremblayi) and potato psyllid (B. nigricornis), it is a member of the complex Bactericera nigricornis Foerster with morphologically and bioecologically very similar species. It was originally described by Hodkinson (1981) as Trioza trigonica based on the analysis of adults collected from the carrots in Portugal, Italy, Cyprus, Turkey, Egypt and Iran. Later it has been transferred to the genus Bactericera together with the two other species from this complex. Adults and larvae B. trigonica, feed on carrot leaf and other Apiaceae. If they appears in large number, they can lead to chlorosis and leaf yellowing, which causes a yield reduction. Besides direct damage, its vector role of bacteria “Candidatus Liberibacter solanacearum” has been determined, which leads to root proliferation and weakening of plants from Apiaceae family due to chlorosis. Ca. Liberibacter solanacearum has been known in North Africa and New Zealand for a long time on plants from Solanacae family, potato in the first place, while in Europe it has been determined on carrot and cellery for the first time, with B. trigonica as its vector. The presence, biology and harmfulness of B. trigonica in the region of Serbia were studyed in the period from 2014 to 2017. It was found in 2014 for the first time and since then, its presence has been determined in all researched localities, during the whole vegetative period of carrot. Besides carrot, B. trigonica has been registered on celery, parsley and wild carrot as well. It has a several generations per year, overwintering as adults., Mrkvina lisna buva Bactericera trigonica pripada familiji Triozidae, nadfamiliji Psylloidea, redu Hemiptera. Zajedno sa lukovom lisnom buvom (B. tremblayi) i krompirovom lisnom buvom (B. nigricornis) pripada kompleksu Bactericera nigricornis Foerster, u morfološkom i bioekološkom smislu veoma sličnih vrsta. Opisana je 1981. godine kao Trioza trigonica na osnovu analize imaga sakupljanih sa mrkve iz Portugalije, Italije, Kipra, Turske, Egipta i Irana. Kasnije je prebačena u rod Bactericera zajedno sa druge dve vrste ovog kompleksa. Imaga i larve se hrane na listu mrkve i drugih štitonoša. Ukoliko se jave u povećanoj brojnosti mogu dovesti do hloroze i žućenja lista, što ima za posledicu smanjenje prinosa. Pored direktnih šteta, utvrđena je njena vektorska uloga fastidiozne bakterije «Candidatus Liberibacter solanacearum», koja na biljkama iz familije Apiaceae dovodi do proliferacije korena i hlorotičnog slabljenja. Ca. L. solanacearum je odavno poznata u Severnoj Americi i Novom Zelandu na biljkama iz familije Solanacae, prevashodno na krompiru, a u Evropi je prvi put utvrđena na mrkvi i celeru, a B. trigonica kao njen vektor. U periodu od 2014. do 2017. godine proučavano je prisustvo, biologija i štetnost B. trigonica na području Srbije. Prvi put je registrovana 2014. godine i od tada je utvrđeno njeno prisustvo u svim lokalitetima istraživanja tokom celog vegetacionog perioda mrkve. Pored mrkve, B. trigonica je registrovana na celeru, peršunu i divljoj mrkvi. U toku godine ima veći broj generacija, prezimljava u stadijumu imaga na skrovitim mestima.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia, Rasprostranjenost, biologija i štetnost mrkvine lisne buve Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) u Srbiji",
pages = "384-375",
number = "4",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4551"
}

Jerinić-Prodanović, D., Obradović, A., Ivanović, M., Prokić, A., Zlatković, N.,& Pavlović, Ž.. (2017). Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(4), 375-384.
https://hdl.handle.net/21.15107/rcub_agrospace_4551

Jerinić-Prodanović D, Obradović A, Ivanović M, Prokić A, Zlatković N, Pavlović Ž. Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia. in Biljni lekar. 2017;45(4):375-384.
https://hdl.handle.net/21.15107/rcub_agrospace_4551 .

Jerinić-Prodanović, Dušanka, Obradović, Aleksa, Ivanović, Milan, Prokić, Andjelka, Zlatković, Nevena, Pavlović, Žaklina, "Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia" in Biljni lekar, 45, no. 4 (2017):375-384,
https://hdl.handle.net/21.15107/rcub_agrospace_4551 .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4556
AB  - Cucurbits production has a long tradition in our country. In some parts of the country, cucumber, watermelon and melon are the most important agricultural crops. High yield and good profit are often compromized by various biotic and abiotic factors. In years with favorable weather conditions cucurbit bacterial diseases can cause serious damage. This paper describes major bacterial diseases of cucurbit plants, such as angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial fruit blotch caused by Acidovorax citrulli, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila and cucurbit yellow wine disease caused by Serratia marcescens, relatively new and invasive disease across the United States. In addition, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae and Pectobacterium carotovorum subsp. carotovorum, inducing bacterial soft rot, are becoming more important in cucurbit production worldwide.
AB  - Biljke familije Cucurbitaceae se dugi niz godina gaje širom naše zemlje. U pojedinim krajevima, proizvodnja krastavca, lubenice i dinje predstavlja osnovni izvor prihoda za mnoga poljoprivredna domaćinstva. Visok prinos i ekonomsku dobit često ugrožavaju različiti biotski i abiotski faktori. U godinama sa uslovima povoljnim za nastanak infekcije, bakteriozna oboljenja mogu prouzrokovati velike gubitke. U ovom radu predstavljene su najznačajnije bakterioze biljaka familije Cucurbitaceae kao što su uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli; zatim bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila i žutilo vreža, čiji je prouzrokovač Serratia marcescens. Gubicima u proizvodnji doprinose i prouzrokovači bakteriozne pegavosti, Pseudomonas syringae pv. syringae, kao i bakteriozne vlažne truleži, Pectobacterium carotovorum subsp. carotovorum.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of Cucurbits
T1  - Bakteriozna oboljenja biljaka familije Cucurbitaceae
EP  - 400
IS  - 4
SP  - 390
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4556
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Kuzmanović, Nemanja and Gašić, Katarina and Ivanović, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Cucurbits production has a long tradition in our country. In some parts of the country, cucumber, watermelon and melon are the most important agricultural crops. High yield and good profit are often compromized by various biotic and abiotic factors. In years with favorable weather conditions cucurbit bacterial diseases can cause serious damage. This paper describes major bacterial diseases of cucurbit plants, such as angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial fruit blotch caused by Acidovorax citrulli, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila and cucurbit yellow wine disease caused by Serratia marcescens, relatively new and invasive disease across the United States. In addition, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae and Pectobacterium carotovorum subsp. carotovorum, inducing bacterial soft rot, are becoming more important in cucurbit production worldwide., Biljke familije Cucurbitaceae se dugi niz godina gaje širom naše zemlje. U pojedinim krajevima, proizvodnja krastavca, lubenice i dinje predstavlja osnovni izvor prihoda za mnoga poljoprivredna domaćinstva. Visok prinos i ekonomsku dobit često ugrožavaju različiti biotski i abiotski faktori. U godinama sa uslovima povoljnim za nastanak infekcije, bakteriozna oboljenja mogu prouzrokovati velike gubitke. U ovom radu predstavljene su najznačajnije bakterioze biljaka familije Cucurbitaceae kao što su uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli; zatim bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila i žutilo vreža, čiji je prouzrokovač Serratia marcescens. Gubicima u proizvodnji doprinose i prouzrokovači bakteriozne pegavosti, Pseudomonas syringae pv. syringae, kao i bakteriozne vlažne truleži, Pectobacterium carotovorum subsp. carotovorum.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of Cucurbits, Bakteriozna oboljenja biljaka familije Cucurbitaceae",
pages = "400-390",
number = "4",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4556"
}

Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Ivanović, M.,& Obradović, A.. (2017). Bacterial diseases of Cucurbits. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(4), 390-400.
https://hdl.handle.net/21.15107/rcub_agrospace_4556

Zlatković N, Prokić A, Kuzmanović N, Gašić K, Ivanović M, Obradović A. Bacterial diseases of Cucurbits. in Biljni lekar. 2017;45(4):390-400.
https://hdl.handle.net/21.15107/rcub_agrospace_4556 .

Zlatković, Nevena, Prokić, Andjelka, Kuzmanović, Nemanja, Gašić, Katarina, Ivanović, Milan, Obradović, Aleksa, "Bacterial diseases of Cucurbits" in Biljni lekar, 45, no. 4 (2017):390-400,
https://hdl.handle.net/21.15107/rcub_agrospace_4556 .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Jerinić-Prodanović, Dušanka
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Pavlović, Žaklina
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4301
AB  - Candidatus Liberibacter solanacearumis a gram-negative bacterium restricted to plant phloem, associated with economically important diseases such as potato zebra chip and yellow decline and with vegetative disorders in carrots and celery. Over the past decade, the zebra chip disease resulted in major economic loses in the potato production of U.S.A., Mexico, Central America and New Zealand. Outbreaks of yellow decline and vegetative disorders in carrot and celery crops have been reported recently in geographically distant areas and the countries of Europe (Finland, Sweden, Norway, Spain, France and Germany). The infected plants showed symptoms including yellow, bronze or red leaf discoloration, reduced size of main root and lateral root proliferation. Given the importance of carrot production in Serbia, the goal of this review paper is to raise awareness about the disease occurrence and distribution, discuss the main characteristics of this relatively unknown pathogen, its detection and identification, epidemiology, symptomatology and disease management, before this emerging plant pathogenic organism reaches our country.
AB  - Fastidiozna bakterija 'Candidatus Liberibacter solanacearum' poznata je kao prouzrokovač destruktivnog oboljenja 'zebrasti čips', a od nedavno i kao prouzrokovač bolesti hlorotičnog slabljenja biljaka iz familije Apiaceae. Kao patogen krompira 'Ca. L. solanacearum' poznat je već deceniju unazad u proizvodnim regionima Amerike i Novog Zelanda. Međutim, na evropskom kontinentu, prisustvo ove bakterije prvi put je potvrđeno na zaraženim biljkama mrkve i celera, što je ukazalo na širi krug domaćina ovog patogena. Areal rasprostranjenja u Evropi obuhvata Finsku, Švedsku, Norvešku, Španiju, Francusku i Nemačku. Kao posledica infekcije dolazi do promena u boji listova, kržljavosti izdanaka, dok najznačajnije štete nastaju usled proliferacije korena. S obzirom na značaj proizvodnje mrkve u našoj zemlji, cilj ovog preglednog rada je da se ukaže na pojavu i rasprostranjenost navedenog destruktivnog oboljenja, karakteristike patogena, simptomatologiju, načine prenošenja, metode detekcije i identifikacije neophodne za pouzdanu i pravovremenu dijagnozu oboljenja, kao i mere zaštite pre nego što ovaj patogen dospe u našu zemlju.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - 'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family
T1  - 'Candidatus Liberibacter solanacearum' - novi patogen biljaka iz familije Apiaceae
EP  - 191
IS  - 2
SP  - 180
VL  - 44
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4301
ER  - 

@article{
author = "Obradović, Aleksa and Jerinić-Prodanović, Dušanka and Ivanović, Milan and Prokić, Andjelka and Kuzmanović, Nemanja and Zlatković, Nevena and Pavlović, Žaklina",
year = "2016",
abstract = "Candidatus Liberibacter solanacearumis a gram-negative bacterium restricted to plant phloem, associated with economically important diseases such as potato zebra chip and yellow decline and with vegetative disorders in carrots and celery. Over the past decade, the zebra chip disease resulted in major economic loses in the potato production of U.S.A., Mexico, Central America and New Zealand. Outbreaks of yellow decline and vegetative disorders in carrot and celery crops have been reported recently in geographically distant areas and the countries of Europe (Finland, Sweden, Norway, Spain, France and Germany). The infected plants showed symptoms including yellow, bronze or red leaf discoloration, reduced size of main root and lateral root proliferation. Given the importance of carrot production in Serbia, the goal of this review paper is to raise awareness about the disease occurrence and distribution, discuss the main characteristics of this relatively unknown pathogen, its detection and identification, epidemiology, symptomatology and disease management, before this emerging plant pathogenic organism reaches our country., Fastidiozna bakterija 'Candidatus Liberibacter solanacearum' poznata je kao prouzrokovač destruktivnog oboljenja 'zebrasti čips', a od nedavno i kao prouzrokovač bolesti hlorotičnog slabljenja biljaka iz familije Apiaceae. Kao patogen krompira 'Ca. L. solanacearum' poznat je već deceniju unazad u proizvodnim regionima Amerike i Novog Zelanda. Međutim, na evropskom kontinentu, prisustvo ove bakterije prvi put je potvrđeno na zaraženim biljkama mrkve i celera, što je ukazalo na širi krug domaćina ovog patogena. Areal rasprostranjenja u Evropi obuhvata Finsku, Švedsku, Norvešku, Španiju, Francusku i Nemačku. Kao posledica infekcije dolazi do promena u boji listova, kržljavosti izdanaka, dok najznačajnije štete nastaju usled proliferacije korena. S obzirom na značaj proizvodnje mrkve u našoj zemlji, cilj ovog preglednog rada je da se ukaže na pojavu i rasprostranjenost navedenog destruktivnog oboljenja, karakteristike patogena, simptomatologiju, načine prenošenja, metode detekcije i identifikacije neophodne za pouzdanu i pravovremenu dijagnozu oboljenja, kao i mere zaštite pre nego što ovaj patogen dospe u našu zemlju.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family, 'Candidatus Liberibacter solanacearum' - novi patogen biljaka iz familije Apiaceae",
pages = "191-180",
number = "2",
volume = "44",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4301"
}

Obradović, A., Jerinić-Prodanović, D., Ivanović, M., Prokić, A., Kuzmanović, N., Zlatković, N.,& Pavlović, Ž.. (2016). 'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 44(2), 180-191.
https://hdl.handle.net/21.15107/rcub_agrospace_4301

Obradović A, Jerinić-Prodanović D, Ivanović M, Prokić A, Kuzmanović N, Zlatković N, Pavlović Ž. 'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family. in Biljni lekar. 2016;44(2):180-191.
https://hdl.handle.net/21.15107/rcub_agrospace_4301 .

Obradović, Aleksa, Jerinić-Prodanović, Dušanka, Ivanović, Milan, Prokić, Andjelka, Kuzmanović, Nemanja, Zlatković, Nevena, Pavlović, Žaklina, "'Candidatus Liberibacter solanacearum': New emerging plant disease of Apiaceae family" in Biljni lekar, 44, no. 2 (2016):180-191,
https://hdl.handle.net/21.15107/rcub_agrospace_4301 .

TY  - CONF
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4098
AB  - Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia
EP  - 719
SP  - 715
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.122
ER  - 

@conference{
author = "Ivanović, Milan and Gašić, K. and Prokić, Andjelka and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2016",
abstract = "Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia",
pages = "719-715",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.122"
}

Ivanović, M., Gašić, K., Prokić, A., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2016). Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing
Int Soc Horticultural Science, Leuven 1., 1139, 715-719.
https://doi.org/10.17660/ActaHortic.2016.1139.122

Ivanović M, Gašić K, Prokić A, Kuzmanović N, Zlatković N, Obradović A. Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing. 2016;1139:715-719.
doi:10.17660/ActaHortic.2016.1139.122 .

Ivanović, Milan, Gašić, K., Prokić, Andjelka, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia" in III Balkan Symposium on Fruit Growing, 1139 (2016):715-719,
https://doi.org/10.17660/ActaHortic.2016.1139.122 . .

TY  - JOUR
AU  - Ivanović, Milan
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4293
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Aleksa Obradović: Compendium of pepper diseases and Compendium of tomato diseases and pests, APS Press
EP  - 204
IS  - 2
SP  - 204
VL  - 44
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4293
ER  - 

@article{
author = "Ivanović, Milan",
year = "2016",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Aleksa Obradović: Compendium of pepper diseases and Compendium of tomato diseases and pests, APS Press",
pages = "204-204",
number = "2",
volume = "44",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4293"
}

Ivanović, M.. (2016). Aleksa Obradović: Compendium of pepper diseases and Compendium of tomato diseases and pests, APS Press. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 44(2), 204-204.
https://hdl.handle.net/21.15107/rcub_agrospace_4293

Ivanović M. Aleksa Obradović: Compendium of pepper diseases and Compendium of tomato diseases and pests, APS Press. in Biljni lekar. 2016;44(2):204-204.
https://hdl.handle.net/21.15107/rcub_agrospace_4293 .

Ivanović, Milan, "Aleksa Obradović: Compendium of pepper diseases and Compendium of tomato diseases and pests, APS Press" in Biljni lekar, 44, no. 2 (2016):204-204,
https://hdl.handle.net/21.15107/rcub_agrospace_4293 .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Biondi, E.
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Bertaccini, Assunta
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4044
AB  - Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall
EP  - 319
IS  - 2
SP  - 311
VL  - 98
DO  - 10.4454/JPP.V98I2.028
ER  - 

@article{
author = "Kuzmanović, Nemanja and Biondi, E. and Ivanović, Milan and Prokić, Andjelka and Zlatković, Nevena and Bertaccini, Assunta and Obradović, Aleksa",
year = "2016",
abstract = "Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall",
pages = "319-311",
number = "2",
volume = "98",
doi = "10.4454/JPP.V98I2.028"
}

Kuzmanović, N., Biondi, E., Ivanović, M., Prokić, A., Zlatković, N., Bertaccini, A.,& Obradović, A.. (2016). Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology
Springer, New York., 98(2), 311-319.
https://doi.org/10.4454/JPP.V98I2.028

Kuzmanović N, Biondi E, Ivanović M, Prokić A, Zlatković N, Bertaccini A, Obradović A. Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology. 2016;98(2):311-319.
doi:10.4454/JPP.V98I2.028 .

Kuzmanović, Nemanja, Biondi, E., Ivanović, Milan, Prokić, Andjelka, Zlatković, Nevena, Bertaccini, Assunta, Obradović, Aleksa, "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall" in Journal of Plant Pathology, 98, no. 2 (2016):311-319,
https://doi.org/10.4454/JPP.V98I2.028 . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3880
AB  - During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014.
AB  - U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial fruit blotch of watermelon in Serbia
T1  - Bakteriozna mrljavost plodova lubenice u Srbiji
EP  - 271
IS  - 3
SP  - 265
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3880
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Kuzmanović, Nemanja and Gašić, Katarina and Šević, Milan and Ivanović, Milan and Obradović, Aleksa",
year = "2015",
abstract = "During the summer of 2014, watermelon producers in the north of Serbia (Srem district), observed the occurrence of irregularly shaped, water-soaked lesions on mature watermelon fruits indicating possible bacterial nature of the disease. Bacteria were isolated from symptomatic samples and identified as Acidovorax citrulli, previously described as a causal agent of bacterial fruit blotch. In the past few years, this pathogen caused considerable damage to the watermelon industry of the United States where, in some commercial production sectors, the loss of marketable fruit reached 90%. In Serbia, it is considered a quarantine pathogen and was not recorded until 2014., U leto 2014. godine, proizvođači lubenice iz sremskog okruga uočili su pojavu simptoma u vidu pega vodenastog izgleda i nepravilnog oblika na zrelim plodovima lubenice, koji su ukazivali na oboljenje bakteriozne prirode. Izolacijom i identifikacijom patogena, utvrđeno je prisustvo bakterije Acidovorax citrulli, prouzrokovača bakteriozne mrljavosti plodova lubenice. Ova vrsta je u skorije vreme dosta dobila na značaju, posebno zbog gubitaka u proizvodnji koji su u pojedinim oblastima Sjedinjenih Američkih Država (SAD) dostizali i 90% od ukupnog prinosa. U Srbiji A. citrulli ima karantinski status i nalazi se na A1 listi Evropske i mediteranske organizacije za zaštitu bilja (EPPO).",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial fruit blotch of watermelon in Serbia, Bakteriozna mrljavost plodova lubenice u Srbiji",
pages = "271-265",
number = "3",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3880"
}

Zlatković, N., Prokić, A., Kuzmanović, N., Gašić, K., Šević, M., Ivanović, M.,& Obradović, A.. (2015). Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 43(3), 265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880

Zlatković N, Prokić A, Kuzmanović N, Gašić K, Šević M, Ivanović M, Obradović A. Bacterial fruit blotch of watermelon in Serbia. in Biljni lekar. 2015;43(3):265-271.
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

Zlatković, Nevena, Prokić, Andjelka, Kuzmanović, Nemanja, Gašić, Katarina, Šević, Milan, Ivanović, Milan, Obradović, Aleksa, "Bacterial fruit blotch of watermelon in Serbia" in Biljni lekar, 43, no. 3 (2015):265-271,
https://hdl.handle.net/21.15107/rcub_agrospace_3880 .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3819
AB  - During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease. Out of 14 strains isolated from raspberry tumors, 12 were identified as tumorigenic Rhizobium rhizogenes, one belonged to Agrobacterium tumefaciens genomic species G8, while the remaining strain formed a separate phylogenetic lineage within A. tumefaciens species complex, different from all known genomic species. All strains investigated harbored nopaline-type of Ti plasmid and showed identical pathogenic properties by inoculating several test plants. However, they were divided into two genetic groups based on PCR-RFLP analysis of Ti plasmid virA-virB2 region. Furthermore, total of nine unique ERIC-PCR profiles were identified among the strains studied. Although strains of R. rhizogenes exhibited similar ERIC-PCR profiles, they were differentiated into six distinct genetic groups. Based on the fact that some genetic groups were composed of strains originating from different geographic areas, it can be assumed that they have a common origin and were probably disseminated by movement of infected plant material.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia
EP  - 713
IS  - 4
SP  - 701
VL  - 142
DO  - 10.1007/s10658-015-0645-4
ER  - 

@article{
author = "Kuzmanović, Nemanja and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "During the last 3 years, crown gall disease was observed in some young raspberry plantations throughout Serbia, causing considerable economic losses. Based on biochemical and physiological tests, PCR targeting the 23S rRNA gene, and 16S rRNA and recA gene sequence analysis, at least two different species were identified as causal agents of disease. Out of 14 strains isolated from raspberry tumors, 12 were identified as tumorigenic Rhizobium rhizogenes, one belonged to Agrobacterium tumefaciens genomic species G8, while the remaining strain formed a separate phylogenetic lineage within A. tumefaciens species complex, different from all known genomic species. All strains investigated harbored nopaline-type of Ti plasmid and showed identical pathogenic properties by inoculating several test plants. However, they were divided into two genetic groups based on PCR-RFLP analysis of Ti plasmid virA-virB2 region. Furthermore, total of nine unique ERIC-PCR profiles were identified among the strains studied. Although strains of R. rhizogenes exhibited similar ERIC-PCR profiles, they were differentiated into six distinct genetic groups. Based on the fact that some genetic groups were composed of strains originating from different geographic areas, it can be assumed that they have a common origin and were probably disseminated by movement of infected plant material.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia",
pages = "713-701",
number = "4",
volume = "142",
doi = "10.1007/s10658-015-0645-4"
}

Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 142(4), 701-713.
https://doi.org/10.1007/s10658-015-0645-4

Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia. in European Journal of Plant Pathology. 2015;142(4):701-713.
doi:10.1007/s10658-015-0645-4 .

Kuzmanović, Nemanja, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia" in European Journal of Plant Pathology, 142, no. 4 (2015):701-713,
https://doi.org/10.1007/s10658-015-0645-4 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3730
AB  - Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.
PB  - Amer Soc Microbiology, Washington
T2  - Microbiology Resource Announcements
T1  - Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330
IS  - 2
VL  - 3
DO  - 10.1128/genomeA.00331-15
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Microbiology Resource Announcements",
title = "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330",
number = "2",
volume = "3",
doi = "10.1128/genomeA.00331-15"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements
Amer Soc Microbiology, Washington., 3(2).
https://doi.org/10.1128/genomeA.00331-15

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements. 2015;3(2).
doi:10.1128/genomeA.00331-15 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330" in Microbiology Resource Announcements, 3, no. 2 (2015),
https://doi.org/10.1128/genomeA.00331-15 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Jones, Jeffrey B.
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3716
AB  - Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).
PB  - Elsevier Gmbh, Munich
T2  - Systematic and Applied Microbiology
T1  - Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum
EP  - 378
IS  - 6
SP  - 373
VL  - 38
DO  - 10.1016/j.syapm.2015.06.001
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Jones, Jeffrey B. and Obradović, Aleksa",
year = "2015",
abstract = "Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).",
publisher = "Elsevier Gmbh, Munich",
journal = "Systematic and Applied Microbiology",
title = "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum",
pages = "378-373",
number = "6",
volume = "38",
doi = "10.1016/j.syapm.2015.06.001"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Jones, J. B.,& Obradović, A.. (2015). Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology
Elsevier Gmbh, Munich., 38(6), 373-378.
https://doi.org/10.1016/j.syapm.2015.06.001

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Jones JB, Obradović A. Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology. 2015;38(6):373-378.
doi:10.1016/j.syapm.2015.06.001 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Jones, Jeffrey B., Obradović, Aleksa, "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum" in Systematic and Applied Microbiology, 38, no. 6 (2015):373-378,
https://doi.org/10.1016/j.syapm.2015.06.001 . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Blagojević, N.
AU  - Obradović, Aleksa
AU  - Gašić, K.
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3522
AB  - In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR
EP  - 84
SP  - 81
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.10
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Andjelka and Blagojević, N. and Obradović, Aleksa and Gašić, K.",
year = "2014",
abstract = "In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR",
pages = "84-81",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.10"
}

Ivanović, M., Kuzmanović, N., Prokić, A., Blagojević, N., Obradović, A.,& Gašić, K.. (2014). Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in Acta Horticulturae
International Society for Horticultural Science., 1056, 81-84.
https://doi.org/10.17660/ActaHortic.2014.1056.10

Ivanović M, Kuzmanović N, Prokić A, Blagojević N, Obradović A, Gašić K. Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in Acta Horticulturae. 2014;1056:81-84.
doi:10.17660/ActaHortic.2014.1056.10 .

Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Andjelka, Blagojević, N., Obradović, Aleksa, Gašić, K., "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR" in Acta Horticulturae, 1056 (2014):81-84,
https://doi.org/10.17660/ActaHortic.2014.1056.10 . .

TY  - JOUR
AU  - Gašić, K.
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Šević, Milan
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3510
AB  - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
EP  - 126
SP  - 123
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.17
ER  - 

@article{
author = "Gašić, K. and Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Andjelka and Obradović, Aleksa and Šević, Milan",
year = "2014",
abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential",
pages = "126-123",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.17"
}

Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae
International Society for Horticultural Science., 1056, 123-126.
https://doi.org/10.17660/ActaHortic.2014.1056.17

Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae. 2014;1056:123-126.
doi:10.17660/ActaHortic.2014.1056.17 .

Gašić, K., Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Andjelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in Acta Horticulturae, 1056 (2014):123-126,
https://doi.org/10.17660/ActaHortic.2014.1056.17 . .