Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia
Само за регистроване кориснике
2023
Аутори
Ivanović, MilanProkić, Anđelka
Gašić, Katarina
Menković, Jelena
Kuzmanović, Nemanja
Zlatković, Nevena
Obradović, Aleksa
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
During May 2016, severe blight symptoms were observed in several
raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic
raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally,
eight raspberry strains, isolated earlier from two similar outbreaks, were
included in the study. Pathogenicity of the strains was confirmed on
detached raspberry and blackberry shoots by reproducing the symptoms
of natural infection. The strains were Gram-negative, fluorescent on
King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic,
arginine dihydrolase negative, and induced hypersensitivity in tobacco
leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all
strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using m...orphology, LOPAT, GATTa, and ice-nucleating ability
tests suggested that isolated strains belong to Pseudomonas syringae.
The syrB gene associated with syringomycin production was detected
in all strains. DNA fingerprints with REP, ERIC, and BOX primers
generated identical profiles for 29 strains, except for strain KBI 222,
which showed a unique genomic fingerprint. In all, 9 of 10 selected
strains exhibited identical sequences of four housekeeping genes: gyrB,
rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in
strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree
based on a concatenated sequence of all four housekeeping genes,
strains clustered within phylogroup 2 (i.e., genomospecies 1) of the
P. syringae species complex, with pathotype strains of P. syringae pv.
aceris and P. syringae pv. solidagae as their closest relatives. There
was no correlation between genotype and geographic origin, particular
outbreak, host, or cultivar.
Кључне речи:
epidemiology / genetic relatedness / multilocus sequence analysis / Pseudomonas blight / repetitive sequence-based PCRИзвор:
Plant Disease, 2023, 107, 826-833Издавач:
- APS Publications
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200010 (Институт за заштиту биља и животну средину, Београд) (RS-MESTD-inst-2020-200010)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Ivanović, Milan AU - Prokić, Anđelka AU - Gašić, Katarina AU - Menković, Jelena AU - Kuzmanović, Nemanja AU - Zlatković, Nevena AU - Obradović, Aleksa PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6326 AB - During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar. PB - APS Publications T2 - Plant Disease T1 - Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia EP - 833 IS - 107 SP - 826 DO - 10.1094/PDIS-06-22-1425-RE ER -
@article{ author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa", year = "2023", abstract = "During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar.", publisher = "APS Publications", journal = "Plant Disease", title = "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia", pages = "833-826", number = "107", doi = "10.1094/PDIS-06-22-1425-RE" }
Ivanović, M., Prokić, A., Gašić, K., Menković, J., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2023). Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease APS Publications.(107), 826-833. https://doi.org/10.1094/PDIS-06-22-1425-RE
Ivanović M, Prokić A, Gašić K, Menković J, Kuzmanović N, Zlatković N, Obradović A. Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease. 2023;(107):826-833. doi:10.1094/PDIS-06-22-1425-RE .
Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia" in Plant Disease, no. 107 (2023):826-833, https://doi.org/10.1094/PDIS-06-22-1425-RE . .