TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Biondi, E.
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Bertaccini, Assunta
AU  - Obradović, Aleksa
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4044
AB  - Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall
EP  - 319
IS  - 2
SP  - 311
VL  - 98
DO  - 10.4454/JPP.V98I2.028
ER  - 

@article{
author = "Kuzmanović, Nemanja and Biondi, E. and Ivanović, Milan and Prokić, Andjelka and Zlatković, Nevena and Bertaccini, Assunta and Obradović, Aleksa",
year = "2016",
abstract = "Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall",
pages = "319-311",
number = "2",
volume = "98",
doi = "10.4454/JPP.V98I2.028"
}

Kuzmanović, N., Biondi, E., Ivanović, M., Prokić, A., Zlatković, N., Bertaccini, A.,& Obradović, A.. (2016). Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology
Springer, New York., 98(2), 311-319.
https://doi.org/10.4454/JPP.V98I2.028

Kuzmanović N, Biondi E, Ivanović M, Prokić A, Zlatković N, Bertaccini A, Obradović A. Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology. 2016;98(2):311-319.
doi:10.4454/JPP.V98I2.028 .

Kuzmanović, Nemanja, Biondi, E., Ivanović, Milan, Prokić, Andjelka, Zlatković, Nevena, Bertaccini, Assunta, Obradović, Aleksa, "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall" in Journal of Plant Pathology, 98, no. 2 (2016):311-319,
https://doi.org/10.4454/JPP.V98I2.028 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Biondi, E.
AU  - Bertaccini, Assunta
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3707
AB  - AimsTo analyse genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an 8-year period and to assess the relative contribution of mutation and recombination in shaping their diversity. Methods and ResultsBy using random amplified polymorphic DNA (RAPD) PCR, strains studied were distributed into 12 genetic groups. Sequence analysis of dnaK, gyrB and recA housekeeping genes was employed to characterize a representative subcollection of 28 strains. A total of 15 different haplotypes were found. Nucleotide sequence analysis suggested the presence of recombination events in A.vitis, particularly affecting dnaK locus. Although prevalence of mutation over recombination was found, impact of recombination was about two times greater than mutation in the evolution of the housekeeping genes analysed. ConclusionsThe RAPD analysis indicated high degree of genetic diversity among the strains. However, the most abundant RAPD group was composed of 35 strains, which could lead to the conclusion that they share a common origin and were distributed by the movement of infected grapevine planting material as a most common way of crossing long distances. Furthermore, it seems that recombination is acting as an important driving force in the evolution of A.vitis. As no substantial evidence of recombination was detected within recA gene fragment, this phylogenetic marker could be reliable to characterize phylogenetic relationships among A.vitis strains. Significance and Impact of the StudyWe demonstrated clear epidemiological relationship between majority of strains studied, suggesting a need for more stringent phytosanitary measures in international trade. Moreover, this is the first study to report recombination in A.vitis.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Genetic relatedness and recombination analysis of Allorhizobium vitis strains associated with grapevine crown gall outbreaks in Europe
EP  - 796
IS  - 3
SP  - 786
VL  - 119
DO  - 10.1111/jam.12858
ER  - 

@article{
author = "Kuzmanović, Nemanja and Biondi, E. and Bertaccini, Assunta and Obradović, Aleksa",
year = "2015",
abstract = "AimsTo analyse genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an 8-year period and to assess the relative contribution of mutation and recombination in shaping their diversity. Methods and ResultsBy using random amplified polymorphic DNA (RAPD) PCR, strains studied were distributed into 12 genetic groups. Sequence analysis of dnaK, gyrB and recA housekeeping genes was employed to characterize a representative subcollection of 28 strains. A total of 15 different haplotypes were found. Nucleotide sequence analysis suggested the presence of recombination events in A.vitis, particularly affecting dnaK locus. Although prevalence of mutation over recombination was found, impact of recombination was about two times greater than mutation in the evolution of the housekeeping genes analysed. ConclusionsThe RAPD analysis indicated high degree of genetic diversity among the strains. However, the most abundant RAPD group was composed of 35 strains, which could lead to the conclusion that they share a common origin and were distributed by the movement of infected grapevine planting material as a most common way of crossing long distances. Furthermore, it seems that recombination is acting as an important driving force in the evolution of A.vitis. As no substantial evidence of recombination was detected within recA gene fragment, this phylogenetic marker could be reliable to characterize phylogenetic relationships among A.vitis strains. Significance and Impact of the StudyWe demonstrated clear epidemiological relationship between majority of strains studied, suggesting a need for more stringent phytosanitary measures in international trade. Moreover, this is the first study to report recombination in A.vitis.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Genetic relatedness and recombination analysis of Allorhizobium vitis strains associated with grapevine crown gall outbreaks in Europe",
pages = "796-786",
number = "3",
volume = "119",
doi = "10.1111/jam.12858"
}

Kuzmanović, N., Biondi, E., Bertaccini, A.,& Obradović, A.. (2015). Genetic relatedness and recombination analysis of Allorhizobium vitis strains associated with grapevine crown gall outbreaks in Europe. in Journal of Applied Microbiology
Wiley, Hoboken., 119(3), 786-796.
https://doi.org/10.1111/jam.12858

Kuzmanović N, Biondi E, Bertaccini A, Obradović A. Genetic relatedness and recombination analysis of Allorhizobium vitis strains associated with grapevine crown gall outbreaks in Europe. in Journal of Applied Microbiology. 2015;119(3):786-796.
doi:10.1111/jam.12858 .

Kuzmanović, Nemanja, Biondi, E., Bertaccini, Assunta, Obradović, Aleksa, "Genetic relatedness and recombination analysis of Allorhizobium vitis strains associated with grapevine crown gall outbreaks in Europe" in Journal of Applied Microbiology, 119, no. 3 (2015):786-796,
https://doi.org/10.1111/jam.12858 . .

TY  - CONF
AU  - Kuzmanović, Nemanja
AU  - Biondi, E.
AU  - Bertaccini, Assunta
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3479
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Genetic diversity of Agrobacterium vitis from diverse geographic origins based on RAPD and recA gene sequence analysis
EP  - 64
IS  - 11
SP  - 64
VL  - 104
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3479
ER  - 

@conference{
author = "Kuzmanović, Nemanja and Biondi, E. and Bertaccini, Assunta and Obradović, Aleksa",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Genetic diversity of Agrobacterium vitis from diverse geographic origins based on RAPD and recA gene sequence analysis",
pages = "64-64",
number = "11",
volume = "104",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3479"
}

Kuzmanović, N., Biondi, E., Bertaccini, A.,& Obradović, A.. (2014). Genetic diversity of Agrobacterium vitis from diverse geographic origins based on RAPD and recA gene sequence analysis. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 64-64.
https://hdl.handle.net/21.15107/rcub_agrospace_3479

Kuzmanović N, Biondi E, Bertaccini A, Obradović A. Genetic diversity of Agrobacterium vitis from diverse geographic origins based on RAPD and recA gene sequence analysis. in Phytopathology. 2014;104(11):64-64.
https://hdl.handle.net/21.15107/rcub_agrospace_3479 .

Kuzmanović, Nemanja, Biondi, E., Bertaccini, Assunta, Obradović, Aleksa, "Genetic diversity of Agrobacterium vitis from diverse geographic origins based on RAPD and recA gene sequence analysis" in Phytopathology, 104, no. 11 (2014):64-64,
https://hdl.handle.net/21.15107/rcub_agrospace_3479 .