TY  - CHAP
AU  - Pulawska, Joanna
AU  - Gétaz, M.
AU  - Kaluzna, M.
AU  - Kuzmanović, Nemanja
AU  - Obradović, Aleksa
AU  - Pothier, J.F.
AU  - Ruinelli, M.
AU  - Boscia, D.
AU  - Saponari, M.
AU  - Végh, A.
AU  - Palkovics, L.
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4505
PB  - CABI International
T2  - Cherries: Botany, Production and Uses
T1  - Bacterial diseases
EP  - 385
SP  - 365
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4505
ER  - 

@inbook{
author = "Pulawska, Joanna and Gétaz, M. and Kaluzna, M. and Kuzmanović, Nemanja and Obradović, Aleksa and Pothier, J.F. and Ruinelli, M. and Boscia, D. and Saponari, M. and Végh, A. and Palkovics, L.",
year = "2017",
publisher = "CABI International",
journal = "Cherries: Botany, Production and Uses",
booktitle = "Bacterial diseases",
pages = "385-365",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4505"
}

Pulawska, J., Gétaz, M., Kaluzna, M., Kuzmanović, N., Obradović, A., Pothier, J.F., Ruinelli, M., Boscia, D., Saponari, M., Végh, A.,& Palkovics, L.. (2017). Bacterial diseases. in Cherries: Botany, Production and Uses
CABI International., 365-385.
https://hdl.handle.net/21.15107/rcub_agrospace_4505

Pulawska J, Gétaz M, Kaluzna M, Kuzmanović N, Obradović A, Pothier J, Ruinelli M, Boscia D, Saponari M, Végh A, Palkovics L. Bacterial diseases. in Cherries: Botany, Production and Uses. 2017;:365-385.
https://hdl.handle.net/21.15107/rcub_agrospace_4505 .

Pulawska, Joanna, Gétaz, M., Kaluzna, M., Kuzmanović, Nemanja, Obradović, Aleksa, Pothier, J.F., Ruinelli, M., Boscia, D., Saponari, M., Végh, A., Palkovics, L., "Bacterial diseases" in Cherries: Botany, Production and Uses (2017):365-385,
https://hdl.handle.net/21.15107/rcub_agrospace_4505 .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3730
AB  - Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.
PB  - Amer Soc Microbiology, Washington
T2  - Microbiology Resource Announcements
T1  - Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330
IS  - 2
VL  - 3
DO  - 10.1128/genomeA.00331-15
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Microbiology Resource Announcements",
title = "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330",
number = "2",
volume = "3",
doi = "10.1128/genomeA.00331-15"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements
Amer Soc Microbiology, Washington., 3(2).
https://doi.org/10.1128/genomeA.00331-15

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements. 2015;3(2).
doi:10.1128/genomeA.00331-15 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330" in Microbiology Resource Announcements, 3, no. 2 (2015),
https://doi.org/10.1128/genomeA.00331-15 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Jones, Jeffrey B.
AU  - Obradović, Aleksa
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3716
AB  - Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).
PB  - Elsevier Gmbh, Munich
T2  - Systematic and Applied Microbiology
T1  - Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum
EP  - 378
IS  - 6
SP  - 373
VL  - 38
DO  - 10.1016/j.syapm.2015.06.001
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Jones, Jeffrey B. and Obradović, Aleksa",
year = "2015",
abstract = "Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).",
publisher = "Elsevier Gmbh, Munich",
journal = "Systematic and Applied Microbiology",
title = "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum",
pages = "378-373",
number = "6",
volume = "38",
doi = "10.1016/j.syapm.2015.06.001"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Jones, J. B.,& Obradović, A.. (2015). Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology
Elsevier Gmbh, Munich., 38(6), 373-378.
https://doi.org/10.1016/j.syapm.2015.06.001

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Jones JB, Obradović A. Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology. 2015;38(6):373-378.
doi:10.1016/j.syapm.2015.06.001 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Jones, Jeffrey B., Obradović, Aleksa, "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum" in Systematic and Applied Microbiology, 38, no. 6 (2015):373-378,
https://doi.org/10.1016/j.syapm.2015.06.001 . .

TY  - JOUR
AU  - Ismail, Emadeldeen
AU  - Blom, Jochen
AU  - Bultreys, Alain
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
AU  - van Doorn, Joop
AU  - Bergsma-Vlami, Maria
AU  - Maes, Martine
AU  - Willems, Anne
AU  - Duffy, Brion
AU  - Stockwell, Virginia O.
AU  - Smits, Theo H.M.
AU  - Pulawska, Joanna
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3470
AB  - Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.
PB  - Springer, New York
T2  - Archives of Microbiology
T1  - A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids
EP  - 899
IS  - 12
SP  - 891
VL  - 196
DO  - 10.1007/s00203-014-1028-5
ER  - 

@article{
author = "Ismail, Emadeldeen and Blom, Jochen and Bultreys, Alain and Ivanović, Milan and Obradović, Aleksa and van Doorn, Joop and Bergsma-Vlami, Maria and Maes, Martine and Willems, Anne and Duffy, Brion and Stockwell, Virginia O. and Smits, Theo H.M. and Pulawska, Joanna",
year = "2014",
abstract = "Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.",
publisher = "Springer, New York",
journal = "Archives of Microbiology",
title = "A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids",
pages = "899-891",
number = "12",
volume = "196",
doi = "10.1007/s00203-014-1028-5"
}

Ismail, E., Blom, J., Bultreys, A., Ivanović, M., Obradović, A., van Doorn, J., Bergsma-Vlami, M., Maes, M., Willems, A., Duffy, B., Stockwell, V. O., Smits, T. H.M.,& Pulawska, J.. (2014). A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids. in Archives of Microbiology
Springer, New York., 196(12), 891-899.
https://doi.org/10.1007/s00203-014-1028-5

Ismail E, Blom J, Bultreys A, Ivanović M, Obradović A, van Doorn J, Bergsma-Vlami M, Maes M, Willems A, Duffy B, Stockwell VO, Smits TH, Pulawska J. A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids. in Archives of Microbiology. 2014;196(12):891-899.
doi:10.1007/s00203-014-1028-5 .

Ismail, Emadeldeen, Blom, Jochen, Bultreys, Alain, Ivanović, Milan, Obradović, Aleksa, van Doorn, Joop, Bergsma-Vlami, Maria, Maes, Martine, Willems, Anne, Duffy, Brion, Stockwell, Virginia O., Smits, Theo H.M., Pulawska, Joanna, "A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids" in Archives of Microbiology, 196, no. 12 (2014):891-899,
https://doi.org/10.1007/s00203-014-1028-5 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Gašić, Katarina
AU  - Blagojević, Nevena
AU  - Pulawska, Joanna
AU  - Obradović, Aleksa
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3302
AB  - Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia
EP  - 16
IS  - 1
SP  - 11
VL  - 137
DO  - 10.1007/s10658-013-0229-0
ER  - 

@article{
author = "Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Andjelka and Gašić, Katarina and Blagojević, Nevena and Pulawska, Joanna and Obradović, Aleksa",
year = "2013",
abstract = "Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia",
pages = "16-11",
number = "1",
volume = "137",
doi = "10.1007/s10658-013-0229-0"
}

Kuzmanović, N., Ivanović, M., Prokić, A., Gašić, K., Blagojević, N., Pulawska, J.,& Obradović, A.. (2013). Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 137(1), 11-16.
https://doi.org/10.1007/s10658-013-0229-0

Kuzmanović N, Ivanović M, Prokić A, Gašić K, Blagojević N, Pulawska J, Obradović A. Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology. 2013;137(1):11-16.
doi:10.1007/s10658-013-0229-0 .

Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Andjelka, Gašić, Katarina, Blagojević, Nevena, Pulawska, Joanna, Obradović, Aleksa, "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia" in European Journal of Plant Pathology, 137, no. 1 (2013):11-16,
https://doi.org/10.1007/s10658-013-0229-0 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Ćalić, Andjelka
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Pulawska, Joanna
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2997
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia
EP  - 286
IS  - 2
SP  - 286
VL  - 96
DO  - 10.1094/PDIS-07-11-0600
ER  - 

@article{
author = "Kuzmanović, Nemanja and Ćalić, Andjelka and Ivanović, Milan and Gašić, K. and Pulawska, Joanna and Obradović, Aleksa",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia",
pages = "286-286",
number = "2",
volume = "96",
doi = "10.1094/PDIS-07-11-0600"
}

Kuzmanović, N., Ćalić, A., Ivanović, M., Gašić, K., Pulawska, J.,& Obradović, A.. (2012). First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(2), 286-286.
https://doi.org/10.1094/PDIS-07-11-0600

Kuzmanović N, Ćalić A, Ivanović M, Gašić K, Pulawska J, Obradović A. First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia. in Plant Disease. 2012;96(2):286-286.
doi:10.1094/PDIS-07-11-0600 .

Kuzmanović, Nemanja, Ćalić, Andjelka, Ivanović, Milan, Gašić, K., Pulawska, Joanna, Obradović, Aleksa, "First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia" in Plant Disease, 96, no. 2 (2012):286-286,
https://doi.org/10.1094/PDIS-07-11-0600 . .