Miletić, D., Karličić, V., Lević, S., Nedović, V., Sknepnek, A., Jovičić-Petrović, J.,& Raičević, V.. (2023). Novi tehnološki postupak dobijanja biofortifikatora na bazi Trichoderma spp. obogaćene selenom za primenu u održivoj poljoprivredi. .
https://hdl.handle.net/21.15107/rcub_agrospace_6495

Miletić D, Karličić V, Lević S, Nedović V, Sknepnek A, Jovičić-Petrović J, Raičević V. Novi tehnološki postupak dobijanja biofortifikatora na bazi Trichoderma spp. obogaćene selenom za primenu u održivoj poljoprivredi. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6495 .

Miletić, Dunja, Karličić, Vera, Lević, Steva, Nedović, Viktor, Sknepnek, Aleksandra, Jovičić-Petrović, Jelena, Raičević, Vera, "Novi tehnološki postupak dobijanja biofortifikatora na bazi Trichoderma spp. obogaćene selenom za primenu u održivoj poljoprivredi" (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6495 .

TY  - CONF
AU  - Hamidović, Saud
AU  - Ibraković, Vanesa
AU  - Duvnjak, Amerisa
AU  - Opačin, Edna
AU  - Karličić, Vera
AU  - Lalević, Blažo
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6533
AB  - One of the major public health concerns is food contamination by pathogenic bacteria. Fresh food
products can be contaminated during each phase of food production. Many factors are involved in the
survival and growth of pathogenic bacteria, such as environmental and agricultural practices. Several
methods are proposed for pathogen removal from fresh food products; one of them is the application
of essential oils. Thus, the aim of this research was to determine the effects of caraway, coriander, and
thyme essential oils on E. coli and Salmonella spp. growth. Mueller-Hinton agar, previously
inoculated by overnight bacterial cultures, was used for the estimation of particular effects. Sterile
filter paper discs impregnated with essential oil were placed on the surface of the agar. After
incubation, the inhibition zone was measured. The olive oil macerate was used as a control. The results
showed that the highest impact was noticed in treatment with caraway essential oil, while thyme
essential oil showed the lowest inhibition zone. The obtained results confirm the potential of natural
products in the suppression of pathogen growth.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022
C3  - Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
T1  - POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6533
ER  - 

@conference{
author = "Hamidović, Saud and Ibraković, Vanesa and Duvnjak, Amerisa and Opačin, Edna and Karličić, Vera and Lalević, Blažo",
year = "2023",
abstract = "One of the major public health concerns is food contamination by pathogenic bacteria. Fresh food
products can be contaminated during each phase of food production. Many factors are involved in the
survival and growth of pathogenic bacteria, such as environmental and agricultural practices. Several
methods are proposed for pathogen removal from fresh food products; one of them is the application
of essential oils. Thus, the aim of this research was to determine the effects of caraway, coriander, and
thyme essential oils on E. coli and Salmonella spp. growth. Mueller-Hinton agar, previously
inoculated by overnight bacterial cultures, was used for the estimation of particular effects. Sterile
filter paper discs impregnated with essential oil were placed on the surface of the agar. After
incubation, the inhibition zone was measured. The olive oil macerate was used as a control. The results
showed that the highest impact was noticed in treatment with caraway essential oil, while thyme
essential oil showed the lowest inhibition zone. The obtained results confirm the potential of natural
products in the suppression of pathogen growth.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022",
journal = "Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection",
title = "POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6533"
}

Hamidović, S., Ibraković, V., Duvnjak, A., Opačin, E., Karličić, V.,& Lalević, B.. (2023). POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY. in Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2022..
https://hdl.handle.net/21.15107/rcub_agrospace_6533

Hamidović S, Ibraković V, Duvnjak A, Opačin E, Karličić V, Lalević B. POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY. in Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6533 .

Hamidović, Saud, Ibraković, Vanesa, Duvnjak, Amerisa, Opačin, Edna, Karličić, Vera, Lalević, Blažo, "POTENTIAL OF PLANT ESSENTIAL OILS IN THE SUPPRESSION OF E. COLI AND SALMONELLA spp. – IN VITRO STUDY" in Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6533 .

TY  - CONF
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Karličič, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Golubović Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6604
AB  - Organochlorinated pesticides (OCPs), such as DDT are characterized as highly persistent and mobile in environment, with high bioaccumulation capacity. The use of those chemicals has been banned in the United States and Europe for decades (Tsai, 2014). Despite this, OCPs-affected ecosystems are detected in several countries, even in the XXI century. Malusa et al. (2020) reported the presence of DDT in more than 80% of soil samples originating from organic farms in Poland. The objective was to determine the impact of microbial consortium on DDT biodegradation in soil. BacFung microbial consortium consisting of Bacillus sp., Pseudomonas sp., Azotobacter sp. and Trichoderma sp. was used for soil inoculation. Soil conditions were improved by grass sowing. The research was conducted at a playground in Tivat (Montenegro), and the size of the total examined area was 3,000 m2 divided into 13 sub-units. The concentration of DDT and its metabolites (DDE - dichlorodiphenyldichloroethylene and DDD - dichlorodiphenyldichloroethane) was measured by GC/MS technique. The initial DDT concentration in soil sub-units varied from 0.005 to 20.5 mg/kg. The sum of DDT+DDD+DDE (ΣDDT ) concentrations varied from 0.024 to 22.7 mg/kg (average concentration was 1.69 mg/kg). After the application of BacFung microbial consortium, the decline of DDT amount in soil sub-units was observed (from 0.005 to 0.057 mg/kg); ΣDDT was 0.020 to 0.2 mg/kg (average
value 0.055 mg/kg). The accumulation of DDD and DDE, as initial degradation products of DDT, was observed. These results showed that microbial consortium BacFung can be used in removal of DDT from polluted environment.
C3  - 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes
T1  - Microbial consortium in in situ remediation of DDT residues-affected soil
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6604
ER  - 

@conference{
author = "Kljujev, Igor and Lalević, Blažo and Karličič, Vera and Jovičić-Petrović, Jelena and Golubović Ćurguz, Vesna and Raičević, Vera",
year = "2023",
abstract = "Organochlorinated pesticides (OCPs), such as DDT are characterized as highly persistent and mobile in environment, with high bioaccumulation capacity. The use of those chemicals has been banned in the United States and Europe for decades (Tsai, 2014). Despite this, OCPs-affected ecosystems are detected in several countries, even in the XXI century. Malusa et al. (2020) reported the presence of DDT in more than 80% of soil samples originating from organic farms in Poland. The objective was to determine the impact of microbial consortium on DDT biodegradation in soil. BacFung microbial consortium consisting of Bacillus sp., Pseudomonas sp., Azotobacter sp. and Trichoderma sp. was used for soil inoculation. Soil conditions were improved by grass sowing. The research was conducted at a playground in Tivat (Montenegro), and the size of the total examined area was 3,000 m2 divided into 13 sub-units. The concentration of DDT and its metabolites (DDE - dichlorodiphenyldichloroethylene and DDD - dichlorodiphenyldichloroethane) was measured by GC/MS technique. The initial DDT concentration in soil sub-units varied from 0.005 to 20.5 mg/kg. The sum of DDT+DDD+DDE (ΣDDT ) concentrations varied from 0.024 to 22.7 mg/kg (average concentration was 1.69 mg/kg). After the application of BacFung microbial consortium, the decline of DDT amount in soil sub-units was observed (from 0.005 to 0.057 mg/kg); ΣDDT was 0.020 to 0.2 mg/kg (average
value 0.055 mg/kg). The accumulation of DDD and DDE, as initial degradation products of DDT, was observed. These results showed that microbial consortium BacFung can be used in removal of DDT from polluted environment.",
journal = "3rd International Meeting on New Strategies in Bioremediation/Restoration Processes",
title = "Microbial consortium in in situ remediation of DDT residues-affected soil",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6604"
}

Kljujev, I., Lalević, B., Karličič, V., Jovičić-Petrović, J., Golubović Ćurguz, V.,& Raičević, V.. (2023). Microbial consortium in in situ remediation of DDT residues-affected soil. in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes, 154.
https://hdl.handle.net/21.15107/rcub_agrospace_6604

Kljujev I, Lalević B, Karličič V, Jovičić-Petrović J, Golubović Ćurguz V, Raičević V. Microbial consortium in in situ remediation of DDT residues-affected soil. in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes. 2023;:154.
https://hdl.handle.net/21.15107/rcub_agrospace_6604 .

Kljujev, Igor, Lalević, Blažo, Karličič, Vera, Jovičić-Petrović, Jelena, Golubović Ćurguz, Vesna, Raičević, Vera, "Microbial consortium in in situ remediation of DDT residues-affected soil" in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes (2023):154,
https://hdl.handle.net/21.15107/rcub_agrospace_6604 .

TY  - CONF
AU  - Dragojević, Milica
AU  - Đokić, Lidija
AU  - Stanković, Nada
AU  - Raičević, Vera
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6605
AB  - Increased soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.
C3  - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE
T1  - Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6605
ER  - 

@conference{
author = "Dragojević, Milica and Đokić, Lidija and Stanković, Nada and Raičević, Vera and Karličič, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "Increased soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6605"
}

Dragojević, M., Đokić, L., Stanković, N., Raičević, V., Karličič, V., Lalević, B.,& Jovičić-Petrović, J.. (2023). Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE.
https://hdl.handle.net/21.15107/rcub_agrospace_6605

Dragojević M, Đokić L, Stanković N, Raičević V, Karličič V, Lalević B, Jovičić-Petrović J. Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6605 .

Dragojević, Milica, Đokić, Lidija, Stanković, Nada, Raičević, Vera, Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6605 .

TY  - JOUR
AU  - Đedović, Suzana
AU  - Stojanova, Monika
AU  - Bojkovski, Jovan
AU  - Kljujev, Igor
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6836
AB  - Microbial degradation, compared with many other degradation processes, is the most important
pathway for the depletion of triazine herbicides in soil. The aim of this study was to determine the
growth potential of Pseudomonas sp. CY in the presence of atrazine and additional carbon (sodium citrate) and nitrogen (ammonium-nitrate) sources. The experiment was performed with five treatments: i) 100 mg/L atrazine (control); ii) One hundred mg/L atrazine + sodium citrate (0.3 %,w/v); iii) One hundred mg/L atrazine + sodium citrate (0.3 %, w/v) + ammonium nitrate (0.6 %, w/v);iv) Atrazine (300 mg/L) + sodium citrate (0.3 %, w/v) and v) Atrazine (500 mg/L) + sodium citrate (0.3 %, w/v). The bacterial count was determined after incubation (7 days at 30°C) using the agar plate method, while atrazine degradation was determined by measuring the optical density at 221 nm.
Pseudomonas sp. CY can partially utilize atrazine as the sole source of carbon and energy. The
highest values of the bacterial count were determined at the highest initial atrazine concentrations; however, bacterial growth was not detected in these treatments. A significant impact of citrate on bacterial growth and atrazine degradation was observed, while the addition of nitrate decreased the atrazine degradation rate. This study confirmed that Pseudomonas sp. CY can be used as a prominent candidate for the remediation of atrazine-affected environments.
PB  - MDPI
T2  - ZEMLJISTE I BILJKA
T1  - Changes in Pseudomonas sp. CY growth in the presence of atrazine
DO  - 10.5937/ZemBilj2302001D
ER  - 

@article{
author = "Đedović, Suzana and Stojanova, Monika and Bojkovski, Jovan and Kljujev, Igor and Karličič, Vera and Lalević, Blažo and Raičević, Vera",
year = "2023",
abstract = "Microbial degradation, compared with many other degradation processes, is the most important
pathway for the depletion of triazine herbicides in soil. The aim of this study was to determine the
growth potential of Pseudomonas sp. CY in the presence of atrazine and additional carbon (sodium citrate) and nitrogen (ammonium-nitrate) sources. The experiment was performed with five treatments: i) 100 mg/L atrazine (control); ii) One hundred mg/L atrazine + sodium citrate (0.3 %,w/v); iii) One hundred mg/L atrazine + sodium citrate (0.3 %, w/v) + ammonium nitrate (0.6 %, w/v);iv) Atrazine (300 mg/L) + sodium citrate (0.3 %, w/v) and v) Atrazine (500 mg/L) + sodium citrate (0.3 %, w/v). The bacterial count was determined after incubation (7 days at 30°C) using the agar plate method, while atrazine degradation was determined by measuring the optical density at 221 nm.
Pseudomonas sp. CY can partially utilize atrazine as the sole source of carbon and energy. The
highest values of the bacterial count were determined at the highest initial atrazine concentrations; however, bacterial growth was not detected in these treatments. A significant impact of citrate on bacterial growth and atrazine degradation was observed, while the addition of nitrate decreased the atrazine degradation rate. This study confirmed that Pseudomonas sp. CY can be used as a prominent candidate for the remediation of atrazine-affected environments.",
publisher = "MDPI",
journal = "ZEMLJISTE I BILJKA",
title = "Changes in Pseudomonas sp. CY growth in the presence of atrazine",
doi = "10.5937/ZemBilj2302001D"
}

Đedović, S., Stojanova, M., Bojkovski, J., Kljujev, I., Karličič, V., Lalević, B.,& Raičević, V.. (2023). Changes in Pseudomonas sp. CY growth in the presence of atrazine. in ZEMLJISTE I BILJKA
MDPI..
https://doi.org/10.5937/ZemBilj2302001D

Đedović S, Stojanova M, Bojkovski J, Kljujev I, Karličič V, Lalević B, Raičević V. Changes in Pseudomonas sp. CY growth in the presence of atrazine. in ZEMLJISTE I BILJKA. 2023;.
doi:10.5937/ZemBilj2302001D .

Đedović, Suzana, Stojanova, Monika, Bojkovski, Jovan, Kljujev, Igor, Karličič, Vera, Lalević, Blažo, Raičević, Vera, "Changes in Pseudomonas sp. CY growth in the presence of atrazine" in ZEMLJISTE I BILJKA (2023),
https://doi.org/10.5937/ZemBilj2302001D . .

TY  - CONF
AU  - Karličič, Vera
AU  - Dragojević, Milica
AU  - Savić, Zagorka
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6606
AB  - Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.
C3  - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE
T1  - Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6606
ER  - 

@conference{
author = "Karličič, Vera and Dragojević, Milica and Savić, Zagorka and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2023",
abstract = "Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6606"
}

Karličič, V., Dragojević, M., Savić, Z., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2023). Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE.
https://hdl.handle.net/21.15107/rcub_agrospace_6606

Karličič V, Dragojević M, Savić Z, Jovičić-Petrović J, Kljujev I, Raičević V. Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

Karličič, Vera, Dragojević, Milica, Savić, Zagorka, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

TY  - CONF
AU  - Karličić, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Raičević, Vera
AU  - Kljujev, Igor
AU  - Gudalović, Tatjana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6511
AB  - One of the major problems in agriculture are Fusarium species which cause fusariosis in wheat,
corn, tomato, melon, watermelon, onion, peas, and beans. Also, Fusarium can synthesize
thermostable mycotoxins which can lead to certain diseases if they were found in food.
Currently, chemical fungicides are mostly used to prevent the occurrence of fusariosis disease,
but the resistance of pathogens to such treatment is being more and more widespread. Soil
microorganisms help in plant defense and growth. The rhizosphere fungi have an important role
in the mutual exchange of nutrients with plants and they can establish specific interactions with
plants. Such rhizosphere fungi are known as plant growth-promoting (PGP). These PGP fungi
affect the better plant growth through the synthesis of certain phytohormones but they also have
the function as biocontrol agents. They can inhibit the growth of phytopathogens through direct
mechanisms of antibiosis, mycoparasitism, and competition.
The goal of this research was an investigation of the antagonistic effect of Trichoderma
harzianum TR1 and Trichoderma citrinoviride 1V on Fusarium oxysporum and Fusarium
graminearum. The antagonistic effect was examined through two tests: Dual culture test (DUAL
test) and the effect of volatile organic compounds (VOCs) of Trichoderma strains on the growth
of Fusarium strains. Also, the cell-wall degrading enzymatic activity of T. citrinoviride and T.
harzianum was examined. The results showed that antagonistic activity of Trichoderma strains
varies from moderate to high. Mycelial growth inhibition by T. citrinoviride was 44% for F.
graminearum and 67% for F. oxysporum. T. harzianum inhibited F. gramminearum growth for
59% and F. oxysporum for 66%. Based on the results, it was concluded that T.harzianum and
T.citrinoviride can be considered biocontrol agents for F. oxysporum and F. graminearum.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - ...
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
T1  - In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6511
ER  - 

@conference{
author = "Karličić, Vera and Jovičić-Petrović, Jelena and Lalević, Blažo and Raičević, Vera and Kljujev, Igor and Gudalović, Tatjana",
year = "2022",
abstract = "One of the major problems in agriculture are Fusarium species which cause fusariosis in wheat,
corn, tomato, melon, watermelon, onion, peas, and beans. Also, Fusarium can synthesize
thermostable mycotoxins which can lead to certain diseases if they were found in food.
Currently, chemical fungicides are mostly used to prevent the occurrence of fusariosis disease,
but the resistance of pathogens to such treatment is being more and more widespread. Soil
microorganisms help in plant defense and growth. The rhizosphere fungi have an important role
in the mutual exchange of nutrients with plants and they can establish specific interactions with
plants. Such rhizosphere fungi are known as plant growth-promoting (PGP). These PGP fungi
affect the better plant growth through the synthesis of certain phytohormones but they also have
the function as biocontrol agents. They can inhibit the growth of phytopathogens through direct
mechanisms of antibiosis, mycoparasitism, and competition.
The goal of this research was an investigation of the antagonistic effect of Trichoderma
harzianum TR1 and Trichoderma citrinoviride 1V on Fusarium oxysporum and Fusarium
graminearum. The antagonistic effect was examined through two tests: Dual culture test (DUAL
test) and the effect of volatile organic compounds (VOCs) of Trichoderma strains on the growth
of Fusarium strains. Also, the cell-wall degrading enzymatic activity of T. citrinoviride and T.
harzianum was examined. The results showed that antagonistic activity of Trichoderma strains
varies from moderate to high. Mycelial growth inhibition by T. citrinoviride was 44% for F.
graminearum and 67% for F. oxysporum. T. harzianum inhibited F. gramminearum growth for
59% and F. oxysporum for 66%. Based on the results, it was concluded that T.harzianum and
T.citrinoviride can be considered biocontrol agents for F. oxysporum and F. graminearum.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, ...",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”",
title = "In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6511"
}

Karličić, V., Jovičić-Petrović, J., Lalević, B., Raičević, V., Kljujev, I.,& Gudalović, T.. (2022). In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6511

Karličić V, Jovičić-Petrović J, Lalević B, Raičević V, Kljujev I, Gudalović T. In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6511 .

Karličić, Vera, Jovičić-Petrović, Jelena, Lalević, Blažo, Raičević, Vera, Kljujev, Igor, Gudalović, Tatjana, "In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6511 .

TY  - JOUR
AU  - Hamidović, Saud R.
AU  - Vukelić, Nikolina M.
AU  - Gavrić, Teofil V.
AU  - Jovičić-Petrović, Jelena P.
AU  - Kljujev, Igor S.
AU  - Karličić, Vera M.
AU  - Lalević, Blažo T.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6513
AB  - Agricultural production has benefited a lot from herbicides; however, the use of herbicides caused
many environmental problems. Herbicide application can affect the biodiversity of an ecosystem by
killing non-target organisms. Microorganisms in the soil are important factors for plant growth; they
represent the biological factor of soil fertility. Herbicides can have a beneficial effect on the
development of some microorganisms and a negative on others, leading to depletion of microbial
diversity in soil. The objective of this work is to determine microbial activity in the soil and to isolate
herbicide-resistant bacteria after the use of the “Stomp” herbicide. Agar plate method was used for the
determination of microbial prevalence in the soil. The results showed an increase in the total number
of bacteria, ammonifiers, fungi, and actinomycetes. Nine isolates, mostly Gram-positive spore-
forming rods, showed an ability to grow in the mineral salt medium with different concentrations of
“Stomp” herbicide. Isolates G1/1 and G1/2, showed high level of tolerance at the initial pendimethalin
concentration of 25 mg/l. Those isolates have the potential to be used to decontaminate herbicide
affected ecosystems.
PB  - Srpsko društvo za proučavanje zemljišta, Beograd
T1  - The effects of the „Stomp“ herbicide application on the microbial prevalence in the soil.
EP  - 23
IS  - 1
SP  - 15
VL  - 71
DO  - DOI:10.5937/ZemBilj2201015H
ER  - 

@article{
author = "Hamidović, Saud R. and Vukelić, Nikolina M. and Gavrić, Teofil V. and Jovičić-Petrović, Jelena P. and Kljujev, Igor S. and Karličić, Vera M. and Lalević, Blažo T.",
year = "2022",
abstract = "Agricultural production has benefited a lot from herbicides; however, the use of herbicides caused
many environmental problems. Herbicide application can affect the biodiversity of an ecosystem by
killing non-target organisms. Microorganisms in the soil are important factors for plant growth; they
represent the biological factor of soil fertility. Herbicides can have a beneficial effect on the
development of some microorganisms and a negative on others, leading to depletion of microbial
diversity in soil. The objective of this work is to determine microbial activity in the soil and to isolate
herbicide-resistant bacteria after the use of the “Stomp” herbicide. Agar plate method was used for the
determination of microbial prevalence in the soil. The results showed an increase in the total number
of bacteria, ammonifiers, fungi, and actinomycetes. Nine isolates, mostly Gram-positive spore-
forming rods, showed an ability to grow in the mineral salt medium with different concentrations of
“Stomp” herbicide. Isolates G1/1 and G1/2, showed high level of tolerance at the initial pendimethalin
concentration of 25 mg/l. Those isolates have the potential to be used to decontaminate herbicide
affected ecosystems.",
publisher = "Srpsko društvo za proučavanje zemljišta, Beograd",
title = "The effects of the „Stomp“ herbicide application on the microbial prevalence in the soil.",
pages = "23-15",
number = "1",
volume = "71",
doi = "DOI:10.5937/ZemBilj2201015H"
}

Hamidović, S. R., Vukelić, N. M., Gavrić, T. V., Jovičić-Petrović, J. P., Kljujev, I. S., Karličić, V. M.,& Lalević, B. T.. (2022). The effects of the „Stomp“ herbicide application on the microbial prevalence in the soil.. 
Srpsko društvo za proučavanje zemljišta, Beograd., 71(1), 15-23.
https://doi.org/DOI:10.5937/ZemBilj2201015H

Hamidović SR, Vukelić NM, Gavrić TV, Jovičić-Petrović JP, Kljujev IS, Karličić VM, Lalević BT. The effects of the „Stomp“ herbicide application on the microbial prevalence in the soil.. 2022;71(1):15-23.
doi:DOI:10.5937/ZemBilj2201015H .

Hamidović, Saud R., Vukelić, Nikolina M., Gavrić, Teofil V., Jovičić-Petrović, Jelena P., Kljujev, Igor S., Karličić, Vera M., Lalević, Blažo T., "The effects of the „Stomp“ herbicide application on the microbial prevalence in the soil.", 71, no. 1 (2022):15-23,
https://doi.org/DOI:10.5937/ZemBilj2201015H . .

TY  - CONF
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6603
AB  - Biopriming is a pre-sowing seed inoculation technique based on beneficial microorganisms. The aim of the study was to estimate biopriming potential of microbial consortium on soybean seeds. Obtained results showed a stimulative effect of biopriming resulting in 8% higher germination percentage. Inoculated seeds were characterised as more vigorous according to vigor I, vigor II and electrical conductivity results. Accelerated aging test decreased germination percentage of both treatments to approximately 66±2%. Inoculated seeds were marked as more vigorous according to vigor I index. Biopriming of soybean seeds resulted in an increase of germination parameters that suggest stimulative effects on seed quality.
C3  - 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia
T1  - Biopriming: multiple effects on soybean germination metrics
EP  - 282
SP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6603
ER  - 

@conference{
author = "Karličič, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2022",
abstract = "Biopriming is a pre-sowing seed inoculation technique based on beneficial microorganisms. The aim of the study was to estimate biopriming potential of microbial consortium on soybean seeds. Obtained results showed a stimulative effect of biopriming resulting in 8% higher germination percentage. Inoculated seeds were characterised as more vigorous according to vigor I, vigor II and electrical conductivity results. Accelerated aging test decreased germination percentage of both treatments to approximately 66±2%. Inoculated seeds were marked as more vigorous according to vigor I index. Biopriming of soybean seeds resulted in an increase of germination parameters that suggest stimulative effects on seed quality.",
journal = "57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia",
title = "Biopriming: multiple effects on soybean germination metrics",
pages = "282-278",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6603"
}

Karličič, V., Lalević, B., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2022). Biopriming: multiple effects on soybean germination metrics. in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia, 278-282.
https://hdl.handle.net/21.15107/rcub_agrospace_6603

Karličič V, Lalević B, Jovičić-Petrović J, Kljujev I, Raičević V. Biopriming: multiple effects on soybean germination metrics. in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia. 2022;:278-282.
https://hdl.handle.net/21.15107/rcub_agrospace_6603 .

Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Biopriming: multiple effects on soybean germination metrics" in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia (2022):278-282,
https://hdl.handle.net/21.15107/rcub_agrospace_6603 .

Raičević, V., Karličič, V., Lalević, B., Jovičić-Petrović, J., Kljujev, I.,& Golubović Ćurguz, V.. (2022). Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola. 
University of Belgrade, Faculty of Agriculture, Serbia..
https://hdl.handle.net/21.15107/rcub_agrospace_6723

Raičević V, Karličič V, Lalević B, Jovičić-Petrović J, Kljujev I, Golubović Ćurguz V. Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6723 .

Raičević, Vera, Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, Kljujev, Igor, Golubović Ćurguz, Vesna, "Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola" (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6723 .

TY  - GEN
AU  - Karličič, Vera
AU  - Simić, Aleksandar
AU  - Brajević, Snežana
AU  - Kljujev, Igor
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6724
AB  - Eksploatacija rude, i pored benefita za ljudsko društvo, dovodi do pogoršanja kvaliteta životne sredine. Kao posledica eksploatacije, dolazi do deponovanja otpadnog materijala i stvaranja jalovina sa nepovoljnim fizičkim, hemijskim i biološkim osobinama. Imajući u vidu značaj mikroorganizama u oštećenim ekosistemima, cilj ovog rada bio je ispitivanje mikrobnog diverziteta jalovine; na jednom delu jalovine izvršena je setva semena crvenog vijuka uz dodatak organskog i mineralnog đubriva (CVF), na drugom setva istog semena bez đubrenja (CV), dok je treći deo, bez setve i đubrenja, predstavljao kontrolu (KON). Mikrobni diverzitet ispitan je metodom agarnih ploča, pri čemu je određen ukupan broj bakterija, amonifikatora i gljiva, kao i prisustvo vrsta iz rodova Pseudomonas sp. i Azotobacter sp. Dobijeni rezultati ukazuju na nizak ukupan broj bakterija, amonifikatora i gljiva, dok vrste iz rodova Pseudomonas sp. i Azotobacter sp. nisu detektovane. Slaba zastupljenost mikrobnih populacija je posledica niskog sadržaja hranljivih supstanci i visoke koncentracije teških metala. Đubrenje je uticalo na povećanje mikrobiološke aktivnosti, kao i na promenu odnosa fungalnih i bakterijskih populacija, što ukazuje na oporavak oštećenog ekosistema.
T2  - XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac
T1  - Mikrobni diverzitet kao pokazatelj remedijacije jalovine
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6724
ER  - 

@misc{
author = "Karličič, Vera and Simić, Aleksandar and Brajević, Snežana and Kljujev, Igor and Jovičić-Petrović, Jelena and Raičević, Vera and Lalević, Blažo",
year = "2022",
abstract = "Eksploatacija rude, i pored benefita za ljudsko društvo, dovodi do pogoršanja kvaliteta životne sredine. Kao posledica eksploatacije, dolazi do deponovanja otpadnog materijala i stvaranja jalovina sa nepovoljnim fizičkim, hemijskim i biološkim osobinama. Imajući u vidu značaj mikroorganizama u oštećenim ekosistemima, cilj ovog rada bio je ispitivanje mikrobnog diverziteta jalovine; na jednom delu jalovine izvršena je setva semena crvenog vijuka uz dodatak organskog i mineralnog đubriva (CVF), na drugom setva istog semena bez đubrenja (CV), dok je treći deo, bez setve i đubrenja, predstavljao kontrolu (KON). Mikrobni diverzitet ispitan je metodom agarnih ploča, pri čemu je određen ukupan broj bakterija, amonifikatora i gljiva, kao i prisustvo vrsta iz rodova Pseudomonas sp. i Azotobacter sp. Dobijeni rezultati ukazuju na nizak ukupan broj bakterija, amonifikatora i gljiva, dok vrste iz rodova Pseudomonas sp. i Azotobacter sp. nisu detektovane. Slaba zastupljenost mikrobnih populacija je posledica niskog sadržaja hranljivih supstanci i visoke koncentracije teških metala. Đubrenje je uticalo na povećanje mikrobiološke aktivnosti, kao i na promenu odnosa fungalnih i bakterijskih populacija, što ukazuje na oporavak oštećenog ekosistema.",
journal = "XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac",
title = "Mikrobni diverzitet kao pokazatelj remedijacije jalovine",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6724"
}

Karličič, V., Simić, A., Brajević, S., Kljujev, I., Jovičić-Petrović, J., Raičević, V.,& Lalević, B.. (2022). Mikrobni diverzitet kao pokazatelj remedijacije jalovine. in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac.
https://hdl.handle.net/21.15107/rcub_agrospace_6724

Karličič V, Simić A, Brajević S, Kljujev I, Jovičić-Petrović J, Raičević V, Lalević B. Mikrobni diverzitet kao pokazatelj remedijacije jalovine. in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6724 .

Karličič, Vera, Simić, Aleksandar, Brajević, Snežana, Kljujev, Igor, Jovičić-Petrović, Jelena, Raičević, Vera, Lalević, Blažo, "Mikrobni diverzitet kao pokazatelj remedijacije jalovine" in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6724 .

TY  - JOUR
AU  - Radić, Danka
AU  - Karličić, Vera
AU  - Đorđević, Jelena
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6116
AB  - A large number of soil microorganisms are characterized as plant growth promoting, but there seems to be a lack of comprehensive knowledge regarding plant growth promoting soil yeasts. The aim of the experiment was to analyse the properties of three yeast species: Schwanniomyces occidentalis BK0302D, Cyberlindnera saturnus CK2404I and Candida tropicalis 2TD2912B, important for plant growth (ammonium sulphate transformation, phosphorus, potassium and zinc dissolution), and to evaluate the effect of yeast on the growth of common wheat and white mustard seedlings after seeds’ inoculation. Common wheat and white mustard seeds were inoculated with the selected yeasts. The final measurements showed that the highest amount of nitrate (10.40 μg mL-1 NO3 −) was produced by C. saturnus CK2404I, while S. occidentalis BK0302D solubilized the largest amount of phosphorus (63.70 μg mL-1 P). All three strains are marked as potassium and zinc solubilizers with both acid and alkaline phosphatase activity. This is the first report on S. occidentalis and C. tropicalis ability to solubilize insoluble potassium and zinc, and C. saturnus ability to solubilize insoluble phosphorus, potassium and zinc. Also, C. tropicalis 2TD2912B exhibited high antagonistic activity (66% growth inhibition) toward Botrytis cinerea. In vivo trial was conducted in a low-nutrient substrate, and S. occidentalis BK0302D was found to have the most considerable influence on common wheat biomass production (34% increase). White mustard inoculation with C. saturnus CK2404I resulted in a 4-fold higher biomass production, while S. occidentalis BK0302D induced a 2-fold increase. The presented results confirmed the multi-functional plant growth promoting characteristics of the tested yeasts and their potential for broad application from conventional agriculture on low-nutrient soils to revegetation of disturbed substrates. © 2022, Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University. All rights reserved.
T2  - Zemdirbyste
T2  - Zemdirbyste
T1  - Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard
EP  - 34
IS  - 1
SP  - 27
VL  - 109
DO  - 10.13080/Z-A.2022.109.004
ER  - 

@article{
author = "Radić, Danka and Karličić, Vera and Đorđević, Jelena and Jovičić-Petrović, Jelena and Kljujev, Igor and Lalević, Blažo and Raičević, Vera",
year = "2022",
abstract = "A large number of soil microorganisms are characterized as plant growth promoting, but there seems to be a lack of comprehensive knowledge regarding plant growth promoting soil yeasts. The aim of the experiment was to analyse the properties of three yeast species: Schwanniomyces occidentalis BK0302D, Cyberlindnera saturnus CK2404I and Candida tropicalis 2TD2912B, important for plant growth (ammonium sulphate transformation, phosphorus, potassium and zinc dissolution), and to evaluate the effect of yeast on the growth of common wheat and white mustard seedlings after seeds’ inoculation. Common wheat and white mustard seeds were inoculated with the selected yeasts. The final measurements showed that the highest amount of nitrate (10.40 μg mL-1 NO3 −) was produced by C. saturnus CK2404I, while S. occidentalis BK0302D solubilized the largest amount of phosphorus (63.70 μg mL-1 P). All three strains are marked as potassium and zinc solubilizers with both acid and alkaline phosphatase activity. This is the first report on S. occidentalis and C. tropicalis ability to solubilize insoluble potassium and zinc, and C. saturnus ability to solubilize insoluble phosphorus, potassium and zinc. Also, C. tropicalis 2TD2912B exhibited high antagonistic activity (66% growth inhibition) toward Botrytis cinerea. In vivo trial was conducted in a low-nutrient substrate, and S. occidentalis BK0302D was found to have the most considerable influence on common wheat biomass production (34% increase). White mustard inoculation with C. saturnus CK2404I resulted in a 4-fold higher biomass production, while S. occidentalis BK0302D induced a 2-fold increase. The presented results confirmed the multi-functional plant growth promoting characteristics of the tested yeasts and their potential for broad application from conventional agriculture on low-nutrient soils to revegetation of disturbed substrates. © 2022, Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University. All rights reserved.",
journal = "Zemdirbyste, Zemdirbyste",
title = "Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard",
pages = "34-27",
number = "1",
volume = "109",
doi = "10.13080/Z-A.2022.109.004"
}

Radić, D., Karličić, V., Đorđević, J., Jovičić-Petrović, J., Kljujev, I., Lalević, B.,& Raičević, V.. (2022). Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard. in Zemdirbyste, 109(1), 27-34.
https://doi.org/10.13080/Z-A.2022.109.004

Radić D, Karličić V, Đorđević J, Jovičić-Petrović J, Kljujev I, Lalević B, Raičević V. Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard. in Zemdirbyste. 2022;109(1):27-34.
doi:10.13080/Z-A.2022.109.004 .

Radić, Danka, Karličić, Vera, Đorđević, Jelena, Jovičić-Petrović, Jelena, Kljujev, Igor, Lalević, Blažo, Raičević, Vera, "Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard" in Zemdirbyste, 109, no. 1 (2022):27-34,
https://doi.org/10.13080/Z-A.2022.109.004 . .

TY  - JOUR
AU  - Kerečki, Slavica
AU  - Pećinar, Ilinka
AU  - Karličić, Vera
AU  - Mirković, Nemanja
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6143
AB  - This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
T2  - Journal of Plant Interactions
T2  - Journal of Plant Interactions
T1  - Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming
EP  - 730
IS  - 1
SP  - 719
VL  - 17
DO  - 10.1080/17429145.2022.2091802
ER  - 

@article{
author = "Kerečki, Slavica and Pećinar, Ilinka and Karličić, Vera and Mirković, Nemanja and Kljujev, Igor and Raičević, Vera and Jovičić-Petrović, Jelena",
year = "2022",
abstract = "This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",
journal = "Journal of Plant Interactions, Journal of Plant Interactions",
title = "Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming",
pages = "730-719",
number = "1",
volume = "17",
doi = "10.1080/17429145.2022.2091802"
}

Kerečki, S., Pećinar, I., Karličić, V., Mirković, N., Kljujev, I., Raičević, V.,& Jovičić-Petrović, J.. (2022). Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming. in Journal of Plant Interactions, 17(1), 719-730.
https://doi.org/10.1080/17429145.2022.2091802

Kerečki S, Pećinar I, Karličić V, Mirković N, Kljujev I, Raičević V, Jovičić-Petrović J. Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming. in Journal of Plant Interactions. 2022;17(1):719-730.
doi:10.1080/17429145.2022.2091802 .

Kerečki, Slavica, Pećinar, Ilinka, Karličić, Vera, Mirković, Nemanja, Kljujev, Igor, Raičević, Vera, Jovičić-Petrović, Jelena, "Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming" in Journal of Plant Interactions, 17, no. 1 (2022):719-730,
https://doi.org/10.1080/17429145.2022.2091802 . .

TY  - CONF
AU  - Kerečki, Slavica
AU  - Jovičić-Petrović, Jelena
AU  - Karličić, Vera
AU  - Kljujev, Igor
AU  - Ćirković, Saša
AU  - Ristić-Đurović, Jasna
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6512
AB  - Seed inoculation (biopriming) represents an agronomic practice directed towards improving
germination, as well as fostering beneficial plant-microbe interaction from the very beginning
of plants’ life. Besides biopriming, static magnetic field (SMF) is studied as an abiotic factor
affecting germination and plant growth. This paper is aimed to examine the combined effect
of Azotobacter chroococcum F8/2 and SMF of 90 mT on germination. A. chroococcum F8/2
has been proven as a successful biopriming agent, with beneficial effect on cucumber,
tomato, wheat, and soybean germination. This research starts from the hypothesis that the
combined effect of Azotobacter inoculation and SMF could lead to synergistic improvement
of germination parameters, compared to already shown effects of biopriming itself. The
research was conducted with following cultivable plants: basil, cucumber, tomato, wheat, and
soybean. Seed treatment was performed by 1h-immersion of surface-sterilized seeds into
bacterial suspension (107CFU/ml), followed by exposure to SMF of 90 mT for 5 min and 15
min. The germination test was conducted with 100 seeds per treatment and lasted 7 days. The
highest improvement of germination percentages was observed in cucumber and basil (an
increase for 35-41% and 41-45%, respectively), compared to biopriming without SMF
treatment. Tomato and wheat germination were not improved by addition of SMF treatment
to biopriming. The obtained results indicate that the application of SMF can affect the
germination parameters that are changed by biopriming. There is a need for further research
in order to explain the differences between plant species’ response.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - ...
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
T1  - Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6512
ER  - 

@conference{
author = "Kerečki, Slavica and Jovičić-Petrović, Jelena and Karličić, Vera and Kljujev, Igor and Ćirković, Saša and Ristić-Đurović, Jasna",
year = "2022",
abstract = "Seed inoculation (biopriming) represents an agronomic practice directed towards improving
germination, as well as fostering beneficial plant-microbe interaction from the very beginning
of plants’ life. Besides biopriming, static magnetic field (SMF) is studied as an abiotic factor
affecting germination and plant growth. This paper is aimed to examine the combined effect
of Azotobacter chroococcum F8/2 and SMF of 90 mT on germination. A. chroococcum F8/2
has been proven as a successful biopriming agent, with beneficial effect on cucumber,
tomato, wheat, and soybean germination. This research starts from the hypothesis that the
combined effect of Azotobacter inoculation and SMF could lead to synergistic improvement
of germination parameters, compared to already shown effects of biopriming itself. The
research was conducted with following cultivable plants: basil, cucumber, tomato, wheat, and
soybean. Seed treatment was performed by 1h-immersion of surface-sterilized seeds into
bacterial suspension (107CFU/ml), followed by exposure to SMF of 90 mT for 5 min and 15
min. The germination test was conducted with 100 seeds per treatment and lasted 7 days. The
highest improvement of germination percentages was observed in cucumber and basil (an
increase for 35-41% and 41-45%, respectively), compared to biopriming without SMF
treatment. Tomato and wheat germination were not improved by addition of SMF treatment
to biopriming. The obtained results indicate that the application of SMF can affect the
germination parameters that are changed by biopriming. There is a need for further research
in order to explain the differences between plant species’ response.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, ...",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”",
title = "Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6512"
}

Kerečki, S., Jovičić-Petrović, J., Karličić, V., Kljujev, I., Ćirković, S.,& Ristić-Đurović, J.. (2022). Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6512

Kerečki S, Jovičić-Petrović J, Karličić V, Kljujev I, Ćirković S, Ristić-Đurović J. Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6512 .

Kerečki, Slavica, Jovičić-Petrović, Jelena, Karličić, Vera, Kljujev, Igor, Ćirković, Saša, Ristić-Đurović, Jasna, "Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6512 .

TY  - CONF
AU  - Kljujev, Igor
AU  - Jovicic-Petrovic, Jelena
AU  - Lalevic, Blazo
AU  - Karlicic, Vera
AU  - Todorovic, Irena
AU  - Prijepoljac, Minela
AU  - Raicevic, Vera
PY  - 2021
UR  - https://iwa-network.org/events/12th-eastern-european-young-water-professionals-conference-water-for-all-water-for-nature-reliable-water-supply-wastewater-treatment/#:~:text=In%202021%20we%20organize%20our,it%20will%20not%20be%20possible.
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6577
AB  - Surface water contamination becomes a raising problem due to different anthropogenic factors such as agriculture, industry, waste landfills, and wastewater effluents. Small rivers are also exposed to wastewater influents, pollution from agricultural activities, as well as raising human influence on the river flow (dam building). This research aimed to analyze the microbiological quality and ecological status of the water on the small river Ribnica in Western Serbia. Ribnica is a 22 km long tributary of Kolubara. The upper part of the river flow is surrounded by forests, agricultural fields, and small farms. Downstream, the river flows through Mionica, a small town where its water quality becomes endangered by communal wastewater, as well as landfills in the river surrounding. The microbiological quality of river water was estimated by the presence of coliforms, E. coli, enterococci which determination was done by Colilert and Enterolert method (IDEXX Laboratories, USA). The other parameters for water ecological status (oligotrophs, heterotrophs microorganisms, and autopurification index) are determined using the serial dilution method and incubation on selective nutrient media. The index of autopurification was calculated as oligotrophs/heterotrophs ratio. Our results show that the greatest pollution comes in the river after Mionica center. The extremely large number of fecal E. coli indicates the presence of fecal matter in water which could be a potential risk for human health if this water is used for irrigation crops or recreation. Also, autopurification index of water in this location indicates very poor self-purification potential which means that this water needs human help for achieving good ecological status. Regular monitoring and analyses of microbiological contamination and ecosystem disturbances should be important input data for public policies and strategic planning in the area of wastewater management, hydraulic engineering, and agricultural management.
C3  - 12th Eastern European Young Water Professionals Conference IWA
T1  - Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6577
ER  - 

@conference{
author = "Kljujev, Igor and Jovicic-Petrovic, Jelena and Lalevic, Blazo and Karlicic, Vera and Todorovic, Irena and Prijepoljac, Minela and Raicevic, Vera",
year = "2021",
abstract = "Surface water contamination becomes a raising problem due to different anthropogenic factors such as agriculture, industry, waste landfills, and wastewater effluents. Small rivers are also exposed to wastewater influents, pollution from agricultural activities, as well as raising human influence on the river flow (dam building). This research aimed to analyze the microbiological quality and ecological status of the water on the small river Ribnica in Western Serbia. Ribnica is a 22 km long tributary of Kolubara. The upper part of the river flow is surrounded by forests, agricultural fields, and small farms. Downstream, the river flows through Mionica, a small town where its water quality becomes endangered by communal wastewater, as well as landfills in the river surrounding. The microbiological quality of river water was estimated by the presence of coliforms, E. coli, enterococci which determination was done by Colilert and Enterolert method (IDEXX Laboratories, USA). The other parameters for water ecological status (oligotrophs, heterotrophs microorganisms, and autopurification index) are determined using the serial dilution method and incubation on selective nutrient media. The index of autopurification was calculated as oligotrophs/heterotrophs ratio. Our results show that the greatest pollution comes in the river after Mionica center. The extremely large number of fecal E. coli indicates the presence of fecal matter in water which could be a potential risk for human health if this water is used for irrigation crops or recreation. Also, autopurification index of water in this location indicates very poor self-purification potential which means that this water needs human help for achieving good ecological status. Regular monitoring and analyses of microbiological contamination and ecosystem disturbances should be important input data for public policies and strategic planning in the area of wastewater management, hydraulic engineering, and agricultural management.",
journal = "12th Eastern European Young Water Professionals Conference IWA",
title = "Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6577"
}

Kljujev, I., Jovicic-Petrovic, J., Lalevic, B., Karlicic, V., Todorovic, I., Prijepoljac, M.,& Raicevic, V.. (2021). Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water. in 12th Eastern European Young Water Professionals Conference IWA.
https://hdl.handle.net/21.15107/rcub_agrospace_6577

Kljujev I, Jovicic-Petrovic J, Lalevic B, Karlicic V, Todorovic I, Prijepoljac M, Raicevic V. Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water. in 12th Eastern European Young Water Professionals Conference IWA. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6577 .

Kljujev, Igor, Jovicic-Petrovic, Jelena, Lalevic, Blazo, Karlicic, Vera, Todorovic, Irena, Prijepoljac, Minela, Raicevic, Vera, "Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water" in 12th Eastern European Young Water Professionals Conference IWA (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6577 .

TY  - CONF
AU  - Kerečki, Slavica
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Karličič, Vera
AU  - Petrović, Ivana
AU  - Raičević, Vera
PY  - 2021
UR  - https://agrosym.ues.rs.ba/article/showpdf/BOOK_OF_PROCEEDINGS_2021_FINAL.pdf
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6578
AB  - Crop yields are highly depended on germination and early stages of plant growth. Numerous
priming techniques are being developed aimed to improve germination. Biopriming represents a
sustainable approach based on seed treatment in bacterial suspension of selected plant growth
promoting strains. One of the most promising plant growth promoting bacteria is Azotobacter
chroococcum. The aim of the research was to evaluate the effects of A. chroococcum F8/2 as a
biopriming agent on germination of various cultivable plants: basil, white mustard, cucumber,
tomato, wheat, canola, and soybean. After surface sterilization, seeds were bioprimed in the
bacterial suspension (107CFU/ml). Uninoculated seeds represented control treatment.
Germination test was conducted with 100 seeds per treatment and the germination was
monitored for 7 days. Following germination parameters were determined: germination
percentage, germination index, mean germination time, vigor I, vigor II, length and dry biomass
of the seedlings. The bacterial inoculation caused higher germination percentages of cucumber,
tomato, wheat and soybean. The highest increase in germination index was observed in wheat
(an increase of 19.8%). Tomato and basil were the only plants where vigor I was not increased
by inoculation. Generally, the most favorable effects of A. chroococcum biopriming were
observed in wheat where vigor I was increased more than twice, and vigor II was higher by
75.4% in inoculated seeds. The results indicate a significant potential for A. chroococcum use in
biopriming. The observed effects of seed priming on germination parameters were crop-specific,
with the most prominent potential in wheat biopriming.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy
PB  - International Society of Environment and Rural Development, Japan
PB  - ...
C3  - Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
T1  - BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6578
ER  - 

@conference{
author = "Kerečki, Slavica and Jovičić-Petrović, Jelena and Kljujev, Igor and Lalević, Blažo and Karličič, Vera and Petrović, Ivana and Raičević, Vera",
year = "2021",
abstract = "Crop yields are highly depended on germination and early stages of plant growth. Numerous
priming techniques are being developed aimed to improve germination. Biopriming represents a
sustainable approach based on seed treatment in bacterial suspension of selected plant growth
promoting strains. One of the most promising plant growth promoting bacteria is Azotobacter
chroococcum. The aim of the research was to evaluate the effects of A. chroococcum F8/2 as a
biopriming agent on germination of various cultivable plants: basil, white mustard, cucumber,
tomato, wheat, canola, and soybean. After surface sterilization, seeds were bioprimed in the
bacterial suspension (107CFU/ml). Uninoculated seeds represented control treatment.
Germination test was conducted with 100 seeds per treatment and the germination was
monitored for 7 days. Following germination parameters were determined: germination
percentage, germination index, mean germination time, vigor I, vigor II, length and dry biomass
of the seedlings. The bacterial inoculation caused higher germination percentages of cucumber,
tomato, wheat and soybean. The highest increase in germination index was observed in wheat
(an increase of 19.8%). Tomato and basil were the only plants where vigor I was not increased
by inoculation. Generally, the most favorable effects of A. chroococcum biopriming were
observed in wheat where vigor I was increased more than twice, and vigor II was higher by
75.4% in inoculated seeds. The results indicate a significant potential for A. chroococcum use in
biopriming. The observed effects of seed priming on germination parameters were crop-specific,
with the most prominent potential in wheat biopriming.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, International Society of Environment and Rural Development, Japan, ...",
journal = "Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”",
title = "BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6578"
}

Kerečki, S., Jovičić-Petrović, J., Kljujev, I., Lalević, B., Karličič, V., Petrović, I.,& Raičević, V.. (2021). BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6578

Kerečki S, Jovičić-Petrović J, Kljujev I, Lalević B, Karličič V, Petrović I, Raičević V. BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6578 .

Kerečki, Slavica, Jovičić-Petrović, Jelena, Kljujev, Igor, Lalević, Blažo, Karličič, Vera, Petrović, Ivana, Raičević, Vera, "BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT" in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021” (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6578 .

TY  - CONF
AU  - Kljujev, Igor
AU  - Karličič, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Veličković, Ana
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6602
AB  - Good microbiological quality of irrigation water has a great importance for health-safety
agricultural production. In order to avoid microbiological contamination of crops, the
microbiological quality of the water supplying the crops should be monitored. The aim of this
paper is investigation of the microbiological quality of the water from 5 channels and one pool
from the municipality of Surcin which can potentially be used for irrigation, as well as
determination the microbiological quality of the vegetables which are irrigated from the pool.
Microbiological parameters of water and vegetables quality are determined by the standard
methods prescribed by the Rulebook on parameters of surface waters (MPN method) and
Rulebook of food hygiene of production of Republic of Serbia. The values of the tested
parameters in one of the five investigated channels, Surcin channel water, were higher in relation
to the limit values of the Rulebook. The increased levels of total and fecal coliforms, Escherichia
coli, Salmonella, and Shigella were found. The Surcin channel water requires appropriate
treatment before its use for crop irrigation. The values of other tested waters did not go beyond
the limit values prescribed by the Rulebook, but for the safer agricultural production their
treatment is recommended. Microbiological analyzes of vegetables were also done. The total and
fecal coliform bacteria, as well as E. coli, Salmonella sp. and Shigella sp., were not detected in
the tested vegetable samples.
C3  - Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
T1  - MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION
EP  - 529
SP  - 523
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6602
ER  - 

@conference{
author = "Kljujev, Igor and Karličič, Vera and Jovičić-Petrović, Jelena and Veličković, Ana and Lalević, Blažo and Raičević, Vera",
year = "2021",
abstract = "Good microbiological quality of irrigation water has a great importance for health-safety
agricultural production. In order to avoid microbiological contamination of crops, the
microbiological quality of the water supplying the crops should be monitored. The aim of this
paper is investigation of the microbiological quality of the water from 5 channels and one pool
from the municipality of Surcin which can potentially be used for irrigation, as well as
determination the microbiological quality of the vegetables which are irrigated from the pool.
Microbiological parameters of water and vegetables quality are determined by the standard
methods prescribed by the Rulebook on parameters of surface waters (MPN method) and
Rulebook of food hygiene of production of Republic of Serbia. The values of the tested
parameters in one of the five investigated channels, Surcin channel water, were higher in relation
to the limit values of the Rulebook. The increased levels of total and fecal coliforms, Escherichia
coli, Salmonella, and Shigella were found. The Surcin channel water requires appropriate
treatment before its use for crop irrigation. The values of other tested waters did not go beyond
the limit values prescribed by the Rulebook, but for the safer agricultural production their
treatment is recommended. Microbiological analyzes of vegetables were also done. The total and
fecal coliform bacteria, as well as E. coli, Salmonella sp. and Shigella sp., were not detected in
the tested vegetable samples.",
journal = "Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”",
title = "MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION",
pages = "529-523",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6602"
}

Kljujev, I., Karličič, V., Jovičić-Petrović, J., Veličković, A., Lalević, B.,& Raičević, V.. (2021). MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, 523-529.
https://hdl.handle.net/21.15107/rcub_agrospace_6602

Kljujev I, Karličič V, Jovičić-Petrović J, Veličković A, Lalević B, Raičević V. MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”. 2021;:523-529.
https://hdl.handle.net/21.15107/rcub_agrospace_6602 .

Kljujev, Igor, Karličič, Vera, Jovičić-Petrović, Jelena, Veličković, Ana, Lalević, Blažo, Raičević, Vera, "MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION" in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021” (2021):523-529,
https://hdl.handle.net/21.15107/rcub_agrospace_6602 .

TY  - JOUR
AU  - Jovičić-Petrović, Jelena
AU  - Karličić, Vera
AU  - Petrović, Ivana
AU  - Ćirković, Saša
AU  - Ristić‐Đurović, Jasna L.
AU  - Raičević, Vera
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5802
AB  - Different priming methods were developed to improve seed germination and the early growth of seedlings. This study aimed to examine the combined effect of bacterial inoculation and static magnetic field on white mustard (Sinapis alba L.) germination. A plant growth‐promoting bacterial strain Bacillus amyloliquefaciens D5 ARV was used for biopriming. The static magnetic field of 90 mT was applied for 5 and 15 min. Analyses of abscisic acid, chlorophyll, anthocyanins, flavonoids content, nitrogen balance index, and bacterial indole‐3‐acetic acid were used to explain observed effects. Bacterial inoculation improved seed germination, whereas exposure to 90 mT for 15 min suppressed germination. Such an unfavorable effect was neutralized when the treatment with the static magnetic field was combined with bacterial inoculation. The highest germination percentage was a result of synergistic action of B. amyloliquefaciens D5 ARV and 15 min long exposure to 90 mT, which induced an increase of 53.20% in the number of germinated seeds. The static magnetic field induced the increase of bacterial indole‐3‐acetic acid production threefold times. Biomagnetic priming caused a metabolic shift from primary to secondary metabolism in the white mustard seedlings. An adequate combination of biological priming and static magnetic field treatment can be successfully used in old seed revitalization and germination improvements.
PB  - Wiley Online Library
T2  - Bio Electro Magnetics
T1  - Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds
DO  - 10.1002/bem.22328
ER  - 

@article{
author = "Jovičić-Petrović, Jelena and Karličić, Vera and Petrović, Ivana and Ćirković, Saša and Ristić‐Đurović, Jasna L. and Raičević, Vera",
year = "2021",
abstract = "Different priming methods were developed to improve seed germination and the early growth of seedlings. This study aimed to examine the combined effect of bacterial inoculation and static magnetic field on white mustard (Sinapis alba L.) germination. A plant growth‐promoting bacterial strain Bacillus amyloliquefaciens D5 ARV was used for biopriming. The static magnetic field of 90 mT was applied for 5 and 15 min. Analyses of abscisic acid, chlorophyll, anthocyanins, flavonoids content, nitrogen balance index, and bacterial indole‐3‐acetic acid were used to explain observed effects. Bacterial inoculation improved seed germination, whereas exposure to 90 mT for 15 min suppressed germination. Such an unfavorable effect was neutralized when the treatment with the static magnetic field was combined with bacterial inoculation. The highest germination percentage was a result of synergistic action of B. amyloliquefaciens D5 ARV and 15 min long exposure to 90 mT, which induced an increase of 53.20% in the number of germinated seeds. The static magnetic field induced the increase of bacterial indole‐3‐acetic acid production threefold times. Biomagnetic priming caused a metabolic shift from primary to secondary metabolism in the white mustard seedlings. An adequate combination of biological priming and static magnetic field treatment can be successfully used in old seed revitalization and germination improvements.",
publisher = "Wiley Online Library",
journal = "Bio Electro Magnetics",
title = "Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds",
doi = "10.1002/bem.22328"
}

Jovičić-Petrović, J., Karličić, V., Petrović, I., Ćirković, S., Ristić‐Đurović, J. L.,& Raičević, V.. (2021). Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds. in Bio Electro Magnetics
Wiley Online Library..
https://doi.org/10.1002/bem.22328

Jovičić-Petrović J, Karličić V, Petrović I, Ćirković S, Ristić‐Đurović JL, Raičević V. Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds. in Bio Electro Magnetics. 2021;.
doi:10.1002/bem.22328 .

Jovičić-Petrović, Jelena, Karličić, Vera, Petrović, Ivana, Ćirković, Saša, Ristić‐Đurović, Jasna L., Raičević, Vera, "Biomagnetic Priming—Possible Strategy to Revitalize OldMustard Seeds" in Bio Electro Magnetics (2021),
https://doi.org/10.1002/bem.22328 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Zlatković, Milica
AU  - Jovičić-Petrović, Jelena
AU  - Nikolić, Milan P.
AU  - Orlović, Saša
AU  - Raičević, Vera
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6021
AB  - Pinus sylvestris bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of P. sylvestris bark against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Botryosphaeriaceae) through its chemical (water extracts) and biological (Trichoderma spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of B. dothidea and D. sarmentorum for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three Trichoderma isolates originating from the pine bark. Trichoderma spp. reduced growth of B. dothidea by 67%–85%, D. sarmentorum by 63%–75% and N. parvum by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of P. sylvestris bark extracts and Trichoderma spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control.
PB  - MDPI
T2  - Forests
T1  - Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae
IS  - 12
SP  - 1731
VL  - 12
DO  - 10.3390/f12121731
ER  - 

@article{
author = "Karličić, Vera and Zlatković, Milica and Jovičić-Petrović, Jelena and Nikolić, Milan P. and Orlović, Saša and Raičević, Vera",
year = "2021",
abstract = "Pinus sylvestris bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of P. sylvestris bark against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Botryosphaeriaceae) through its chemical (water extracts) and biological (Trichoderma spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of B. dothidea and D. sarmentorum for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three Trichoderma isolates originating from the pine bark. Trichoderma spp. reduced growth of B. dothidea by 67%–85%, D. sarmentorum by 63%–75% and N. parvum by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of P. sylvestris bark extracts and Trichoderma spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control.",
publisher = "MDPI",
journal = "Forests",
title = "Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae",
number = "12",
pages = "1731",
volume = "12",
doi = "10.3390/f12121731"
}

Karličić, V., Zlatković, M., Jovičić-Petrović, J., Nikolić, M. P., Orlović, S.,& Raičević, V.. (2021). Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae. in Forests
MDPI., 12(12), 1731.
https://doi.org/10.3390/f12121731

Karličić V, Zlatković M, Jovičić-Petrović J, Nikolić MP, Orlović S, Raičević V. Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae. in Forests. 2021;12(12):1731.
doi:10.3390/f12121731 .

Karličić, Vera, Zlatković, Milica, Jovičić-Petrović, Jelena, Nikolić, Milan P., Orlović, Saša, Raičević, Vera, "Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae" in Forests, 12, no. 12 (2021):1731,
https://doi.org/10.3390/f12121731 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5474
AB  - Red clover (Trifolium pratense L.) seeds were inoculated with several plant growth-promoting bacteria (PGPB) and sown in the substrate contaminated with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organometallic derivatives of tin (OT). The aim was to determine if selected PGPB strains can promote the growth of red clover in the substrate contaminated with several organic pollutants. The influence of bacteria on red clover growth (height, root length and biomass) was monitored during the three-month experimental period. The most significant improvements of seedling height were noted in the treatment with Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. Root growth was positively affected by Serratia liquefaciens Z-I ARV. The same isolates significantly affected biomass production. Those isolates caused total biomass increases of 70%, 48% and 33% compared to control. Bacterial strains used in this study were already confirmed as PGPB by biochemical testing, as well as by an in vivo test of mixed inoculums on several woody plants grown in the coal-mine overburden site. This work is the first-time record on their individual effects on one plant species. Obtained results confirmed that inoculation with several PGPB strains can enhance red clover growth in polluted soil.
AB  - Seme crvene deteline (Trifolium pratense L.), inokulisno sa nekoliko bakterija stimulatora biljnog rasta (PGPB), posejano je u supstrat kontaminiran policikličnim aromatičnim ugljovodonicima (PAHs), polihlorovanim bifenilima (PCBs) i organometalnim derivatima kalaja (OT). Cilj je bio da se utvrdi da li selektovane PGPB mogu promovisati rast crvene deteline u supstratu kontaminiranom sa nekoliko organskih zagađujućih materija. Uticaj bakterija na rast crvene deteline (visina, dužina korena i biomasa) praćen je tri meseca. Najveća visina je zabeležena kod biljaka inokulisanih sa Bacillus amyloliquefaciens D5 ARV i Pseudomonas putida P1 ARV. Rast korena je stimulisan od strane Serratia liquefaciens Z-I ARV. Ovi izolati su značajno uticali i na produkciju biomase. Ukupna biomasa dobijena tokom celog ogleda je za 70%, 48% i 33% veća u odnosu na kontrolu. Bakterijski sojevi korišćeni u ovoj studiji su prethodno potvrđeni kao PGPB kroz biohemijske i in vivo testove mešanog inokuluma na nekoliko drvenastih vrsta gajenih u jalovini. Ovaj rad prvi put beleži njihove pojedinačne efekte na jednu biljnu vrstu. Dobijeni rezultati potvrđuju da inokulacija sa nekoliko PGPB sojeva može ubrzati rast crvene deteline u zagađenom zemljištu.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Bacterial inoculation: A tool for red clover growth promotion in polluted soil
T1  - Bakterijska inokulacija - postupak za stimulaciju rasta crvene deteline gajene u zagađenom zemljištu
EP  - 174
IS  - 2
SP  - 163
VL  - 65
DO  - 10.2298/JAS2002163K
ER  - 

@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Raičević, Vera",
year = "2020",
abstract = "Red clover (Trifolium pratense L.) seeds were inoculated with several plant growth-promoting bacteria (PGPB) and sown in the substrate contaminated with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organometallic derivatives of tin (OT). The aim was to determine if selected PGPB strains can promote the growth of red clover in the substrate contaminated with several organic pollutants. The influence of bacteria on red clover growth (height, root length and biomass) was monitored during the three-month experimental period. The most significant improvements of seedling height were noted in the treatment with Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. Root growth was positively affected by Serratia liquefaciens Z-I ARV. The same isolates significantly affected biomass production. Those isolates caused total biomass increases of 70%, 48% and 33% compared to control. Bacterial strains used in this study were already confirmed as PGPB by biochemical testing, as well as by an in vivo test of mixed inoculums on several woody plants grown in the coal-mine overburden site. This work is the first-time record on their individual effects on one plant species. Obtained results confirmed that inoculation with several PGPB strains can enhance red clover growth in polluted soil., Seme crvene deteline (Trifolium pratense L.), inokulisno sa nekoliko bakterija stimulatora biljnog rasta (PGPB), posejano je u supstrat kontaminiran policikličnim aromatičnim ugljovodonicima (PAHs), polihlorovanim bifenilima (PCBs) i organometalnim derivatima kalaja (OT). Cilj je bio da se utvrdi da li selektovane PGPB mogu promovisati rast crvene deteline u supstratu kontaminiranom sa nekoliko organskih zagađujućih materija. Uticaj bakterija na rast crvene deteline (visina, dužina korena i biomasa) praćen je tri meseca. Najveća visina je zabeležena kod biljaka inokulisanih sa Bacillus amyloliquefaciens D5 ARV i Pseudomonas putida P1 ARV. Rast korena je stimulisan od strane Serratia liquefaciens Z-I ARV. Ovi izolati su značajno uticali i na produkciju biomase. Ukupna biomasa dobijena tokom celog ogleda je za 70%, 48% i 33% veća u odnosu na kontrolu. Bakterijski sojevi korišćeni u ovoj studiji su prethodno potvrđeni kao PGPB kroz biohemijske i in vivo testove mešanog inokuluma na nekoliko drvenastih vrsta gajenih u jalovini. Ovaj rad prvi put beleži njihove pojedinačne efekte na jednu biljnu vrstu. Dobijeni rezultati potvrđuju da inokulacija sa nekoliko PGPB sojeva može ubrzati rast crvene deteline u zagađenom zemljištu.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Bacterial inoculation: A tool for red clover growth promotion in polluted soil, Bakterijska inokulacija - postupak za stimulaciju rasta crvene deteline gajene u zagađenom zemljištu",
pages = "174-163",
number = "2",
volume = "65",
doi = "10.2298/JAS2002163K"
}

Karličić, V., Radić, D., Jovičić-Petrović, J.,& Raičević, V.. (2020). Bacterial inoculation: A tool for red clover growth promotion in polluted soil. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 65(2), 163-174.
https://doi.org/10.2298/JAS2002163K

Karličić V, Radić D, Jovičić-Petrović J, Raičević V. Bacterial inoculation: A tool for red clover growth promotion in polluted soil. in Journal of Agricultural Sciences (Belgrade). 2020;65(2):163-174.
doi:10.2298/JAS2002163K .

Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Raičević, Vera, "Bacterial inoculation: A tool for red clover growth promotion in polluted soil" in Journal of Agricultural Sciences (Belgrade), 65, no. 2 (2020):163-174,
https://doi.org/10.2298/JAS2002163K . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Morina, Filis
AU  - Golubović-Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4466
AB  - Overburden waste dumps represent a huge threat to environmental quality. The reduction of their negative impact can be achieved by vegetation cover establishment. Usually, this action is complicated due to site-specific characteristics, such as nutrient deficiency, elevated metal concentration, low pH value, lack of moisture and lack of organic matter. Establishment of vegetation can be facilitated by inoculation with plant growth promoting bacteria (PGPB) which improve the physicochemical and biological properties of degraded substrates and make them more hospitable for plants. In this study we selected several strains based on the ability to produce ammonia, indole-3-acetic acid, siderophores and lytic enzymes, and to solubilize inorganic phosphates. This selection resulted in microbial consortia consisting of Serratia liquefaciens Z-I ARV, Ensifer adhaerens 10_ARV, Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. The effects of PGPB consortia on one-year-old London plane (Platanus x acerifolia [Aiton] Willd.) seedlings replanted into overburden waste from Kolubara Mine Basin were examined. After seven months, inoculated seedlings were 32% higher with 45% wider root collar diameter and over 80% higher total dry biomass compared to uninoculated seedlings grown in Kolubara's overburden. Inoculation resulted in higher amounts of total soluble proteins, higher chlorophyll and epidermal flavonoids content and higher total antioxidative capacity in the leaves. This study represents a successful search for effective PGPB strains and shows that microbial consortia have an important role in enhancing the growth of seedlings in nutrient deficient and degraded substrates such as overburden waste from open-pit coal mines. Positive response of London plane seedlings suggest that inoculation may help widening the opus of species for reforestation of post mining areas and speed up natural succession processes and recovery of degraded landscapes.
PB  - Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza
T2  - Iforest-Biogeosciences and Forestry
T1  - Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia
EP  - 699
SP  - 692
VL  - 10
DO  - 10.3832/ifor2135-010
ER  - 

@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Lalević, Blažo and Morina, Filis and Golubović-Ćurguz, Vesna and Raičević, Vera",
year = "2017",
abstract = "Overburden waste dumps represent a huge threat to environmental quality. The reduction of their negative impact can be achieved by vegetation cover establishment. Usually, this action is complicated due to site-specific characteristics, such as nutrient deficiency, elevated metal concentration, low pH value, lack of moisture and lack of organic matter. Establishment of vegetation can be facilitated by inoculation with plant growth promoting bacteria (PGPB) which improve the physicochemical and biological properties of degraded substrates and make them more hospitable for plants. In this study we selected several strains based on the ability to produce ammonia, indole-3-acetic acid, siderophores and lytic enzymes, and to solubilize inorganic phosphates. This selection resulted in microbial consortia consisting of Serratia liquefaciens Z-I ARV, Ensifer adhaerens 10_ARV, Bacillus amyloliquefaciens D5 ARV and Pseudomonas putida P1 ARV. The effects of PGPB consortia on one-year-old London plane (Platanus x acerifolia [Aiton] Willd.) seedlings replanted into overburden waste from Kolubara Mine Basin were examined. After seven months, inoculated seedlings were 32% higher with 45% wider root collar diameter and over 80% higher total dry biomass compared to uninoculated seedlings grown in Kolubara's overburden. Inoculation resulted in higher amounts of total soluble proteins, higher chlorophyll and epidermal flavonoids content and higher total antioxidative capacity in the leaves. This study represents a successful search for effective PGPB strains and shows that microbial consortia have an important role in enhancing the growth of seedlings in nutrient deficient and degraded substrates such as overburden waste from open-pit coal mines. Positive response of London plane seedlings suggest that inoculation may help widening the opus of species for reforestation of post mining areas and speed up natural succession processes and recovery of degraded landscapes.",
publisher = "Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza",
journal = "Iforest-Biogeosciences and Forestry",
title = "Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia",
pages = "699-692",
volume = "10",
doi = "10.3832/ifor2135-010"
}

Karličić, V., Radić, D., Jovičić-Petrović, J., Lalević, B., Morina, F., Golubović-Ćurguz, V.,& Raičević, V.. (2017). Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia. in Iforest-Biogeosciences and Forestry
Sisef-Soc Italiana Selvicoltura Ecol Forestale, Potenza., 10, 692-699.
https://doi.org/10.3832/ifor2135-010

Karličić V, Radić D, Jovičić-Petrović J, Lalević B, Morina F, Golubović-Ćurguz V, Raičević V. Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia. in Iforest-Biogeosciences and Forestry. 2017;10:692-699.
doi:10.3832/ifor2135-010 .

Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Lalević, Blažo, Morina, Filis, Golubović-Ćurguz, Vesna, Raičević, Vera, "Use of overburden waste for London plane (Platanus x acerifolia) growth: the role of plant growth promoting microbial consortia" in Iforest-Biogeosciences and Forestry, 10 (2017):692-699,
https://doi.org/10.3832/ifor2135-010 . .

TY  - JOUR
AU  - Radić, Danka
AU  - Pavlović, Vera P.
AU  - Lazović, Milana
AU  - Jovičić-Petrović, Jelena
AU  - Karličić, Vera
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4484
AB  - Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism
EP  - 21893
IS  - 27
SP  - 21885
VL  - 24
DO  - 10.1007/s11356-017-9817-4
ER  - 

@article{
author = "Radić, Danka and Pavlović, Vera P. and Lazović, Milana and Jovičić-Petrović, Jelena and Karličić, Vera and Lalević, Blažo and Raičević, Vera",
year = "2017",
abstract = "Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism",
pages = "21893-21885",
number = "27",
volume = "24",
doi = "10.1007/s11356-017-9817-4"
}

Radić, D., Pavlović, V. P., Lazović, M., Jovičić-Petrović, J., Karličić, V., Lalević, B.,& Raičević, V.. (2017). Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(27), 21885-21893.
https://doi.org/10.1007/s11356-017-9817-4

Radić D, Pavlović VP, Lazović M, Jovičić-Petrović J, Karličić V, Lalević B, Raičević V. Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research. 2017;24(27):21885-21893.
doi:10.1007/s11356-017-9817-4 .

Radić, Danka, Pavlović, Vera P., Lazović, Milana, Jovičić-Petrović, Jelena, Karličić, Vera, Lalević, Blažo, Raičević, Vera, "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism" in Environmental Science and Pollution Research, 24, no. 27 (2017):21885-21893,
https://doi.org/10.1007/s11356-017-9817-4 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Živanović, Iva
AU  - Matijašević, Danka
AU  - Raičević, Vera
AU  - Nikšić, Miomir
AU  - Rac, Vladislav
AU  - Simić, Aleksandar
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4607
AB  - Zeolites can mitigate ammonium losses to the environment by taking up the ions from sources such as farmyard manure and ammonium-bearing fertilizers. This ability can improve nitrogen fertilizing practice given that ammonium ions loaded onto zeolite are available for plant uptake. The main goal of this study was to assess the influence of zeolite (clinoptilolite) and ammonia-loaded clinoptilolite on growth and yield of red clover (Trifolium pratense L.), as well as their influence on the microbial dynamics in soil. Plants sown in pots were cut four times, and dry matter yield (DM) of each harvest was weighed. The number of culturable bacteria, moulds, ammonifiers, Azotobacter sp. and actinomycetes was determined at the beginning of the experiment, and after each harvest. Two control treatments (soil without fertilizer and soil supplemented with mineral fertilizer - CAN) were included in the study. The application of ammonia-loaded clinoptilolite positively and significantly affected the microbial activity and provided higher yields (from 4.9 g/pot in ammonia-loaded clinoptilolite treatment to 4.3 g/pot in soil with zeolite treatment). Our results suggest that the addition of clinoptilolite to organic fertilizers (manure, composts) could be recommended. The increase of microbial communities’ abundances and their activity represented the key benefit from clinoptilolite application.
AB  - Zeoliti mogu ublažiti otpuštanje amonijaka u prirodu preko uzimanja jona iz izvora kao što su stajnjak i đubriva bogata amonijakom. Ova sposobnost može unaprediti đubrenje azotom preko zeolitom usvojenih amonijum jona koji bi tako postali dostupni za usvajanje biljkama. Cilj ovih ispitivanja je bio procena uticaja zeolita (klinoptilolita) i zeolita obogaćenog amonijakom na rast i prinos crvene deteline (Trifolium pratense L.), kao i na njihovu ulogu u mikrobiološkoj dinamici zemljišta. Biljke sejane u sudovima su košene četiri puta i meren je prinos suve materije (SM) svakog otkosa. Broj bakterija, plesni, amonifikatora, Azotobacter sp. i aktinomiceta je utvrđen na početku ogleda i nakon svakog košenja. U istraživanja su uključena dva kontrolna tretmana (zemljište bez đubriva i zemljište sa dodatim mineralnim đubrivom - KAN). Primena klinoptilolita obogaćenog sa amonijakom je pozitivno i značajno uticala na mikrobiološku aktivnost i povećala je prinos (od 4,9 g po sudu kod tretmana sa klinoptilolitom obogaćenim amonijakom do 4,3 g po sudu u zemljištu sa zemljištem i zeolitom zajedno). Naša istraživanja sugerišu da se može preporučiti dodavanje klinoptilolita organskim đubrivima (stajnjaku, kompostu). Ključna dobit od primene klinoptilolita bi se ogledala u povećanju zastupljenosti mikrobioloških zajednica i njihove aktivnosti.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth
T1  - Stimulacija zemljišne mikrobiološke aktivnosti sa klinoptilolitom - uticaj na rast biljke
EP  - 123
IS  - 3
SP  - 117
VL  - 54
DO  - 10.5937/ratpov54-14870
ER  - 

@article{
author = "Karličić, Vera and Živanović, Iva and Matijašević, Danka and Raičević, Vera and Nikšić, Miomir and Rac, Vladislav and Simić, Aleksandar",
year = "2017",
abstract = "Zeolites can mitigate ammonium losses to the environment by taking up the ions from sources such as farmyard manure and ammonium-bearing fertilizers. This ability can improve nitrogen fertilizing practice given that ammonium ions loaded onto zeolite are available for plant uptake. The main goal of this study was to assess the influence of zeolite (clinoptilolite) and ammonia-loaded clinoptilolite on growth and yield of red clover (Trifolium pratense L.), as well as their influence on the microbial dynamics in soil. Plants sown in pots were cut four times, and dry matter yield (DM) of each harvest was weighed. The number of culturable bacteria, moulds, ammonifiers, Azotobacter sp. and actinomycetes was determined at the beginning of the experiment, and after each harvest. Two control treatments (soil without fertilizer and soil supplemented with mineral fertilizer - CAN) were included in the study. The application of ammonia-loaded clinoptilolite positively and significantly affected the microbial activity and provided higher yields (from 4.9 g/pot in ammonia-loaded clinoptilolite treatment to 4.3 g/pot in soil with zeolite treatment). Our results suggest that the addition of clinoptilolite to organic fertilizers (manure, composts) could be recommended. The increase of microbial communities’ abundances and their activity represented the key benefit from clinoptilolite application., Zeoliti mogu ublažiti otpuštanje amonijaka u prirodu preko uzimanja jona iz izvora kao što su stajnjak i đubriva bogata amonijakom. Ova sposobnost može unaprediti đubrenje azotom preko zeolitom usvojenih amonijum jona koji bi tako postali dostupni za usvajanje biljkama. Cilj ovih ispitivanja je bio procena uticaja zeolita (klinoptilolita) i zeolita obogaćenog amonijakom na rast i prinos crvene deteline (Trifolium pratense L.), kao i na njihovu ulogu u mikrobiološkoj dinamici zemljišta. Biljke sejane u sudovima su košene četiri puta i meren je prinos suve materije (SM) svakog otkosa. Broj bakterija, plesni, amonifikatora, Azotobacter sp. i aktinomiceta je utvrđen na početku ogleda i nakon svakog košenja. U istraživanja su uključena dva kontrolna tretmana (zemljište bez đubriva i zemljište sa dodatim mineralnim đubrivom - KAN). Primena klinoptilolita obogaćenog sa amonijakom je pozitivno i značajno uticala na mikrobiološku aktivnost i povećala je prinos (od 4,9 g po sudu kod tretmana sa klinoptilolitom obogaćenim amonijakom do 4,3 g po sudu u zemljištu sa zemljištem i zeolitom zajedno). Naša istraživanja sugerišu da se može preporučiti dodavanje klinoptilolita organskim đubrivima (stajnjaku, kompostu). Ključna dobit od primene klinoptilolita bi se ogledala u povećanju zastupljenosti mikrobioloških zajednica i njihove aktivnosti.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth, Stimulacija zemljišne mikrobiološke aktivnosti sa klinoptilolitom - uticaj na rast biljke",
pages = "123-117",
number = "3",
volume = "54",
doi = "10.5937/ratpov54-14870"
}

Karličić, V., Živanović, I., Matijašević, D., Raičević, V., Nikšić, M., Rac, V.,& Simić, A.. (2017). Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(3), 117-123.
https://doi.org/10.5937/ratpov54-14870

Karličić V, Živanović I, Matijašević D, Raičević V, Nikšić M, Rac V, Simić A. Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth. in Ratarstvo i povrtarstvo. 2017;54(3):117-123.
doi:10.5937/ratpov54-14870 .

Karličić, Vera, Živanović, Iva, Matijašević, Danka, Raičević, Vera, Nikšić, Miomir, Rac, Vladislav, Simić, Aleksandar, "Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth" in Ratarstvo i povrtarstvo, 54, no. 3 (2017):117-123,
https://doi.org/10.5937/ratpov54-14870 . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Radić, Danka
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Jovanović, Ljubinko
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4247
AB  - Plant growth promoting (PGP) bacteria and yeasts play an important role in bioremediation processes. Thirty bacterial and ten yeast isolates were obtained from PAH and PCB contaminated soil with an aim of determining the presence of PGP mechanisms (production of ammonia, indoleacetic acid, siderophores and solubilization of inorganic phosphate). As a result, three bacterial (Serratia liquefaciens, Micrococcus sp. and Serratia sp.) and two yeast isolates (Candida utilis and Candida tropicalis) were recognized as PGP strains. Among them, Serratia sp. showed the highest indole production (25.5 μg/ml). Analyses of metal tolerance (Cu+2, Cr+6 and Ni+2) revealed that Serratia liquefaciens, Micrococcus sp., Serratia sp. and Candida tropicalis were capable to tolerate significant concentration of metals. As a result of this study several bacterial and yeast strains were attributed as potential plant growth promoters which can be applied in future remediation activities and environmental quality improvements.
AB  - Zemljište predstavlja dinamičan ekosistem naseljen brojnim mikroorganizmima među kojima su bakterije najbrojnije. Najveći broj je skoncentrisan u uskoj zoni zemljišta koja okružuje koren i koja se naziva rizosfera. Procenjuje se da 1-2% bakterija koje naseljavaju ovu zonu imaju sposobnost da promovišu rast biljaka (engl. plant growth promoting bacteria - PGPB). Pored bakterija, sve više je podataka da i zemljišni kvasci poseduju ove sposobnosti. Mikroorganizmi koji stimulišu rast biljaka koriste različite mehanizme kojima povećavaju dostupnost nutrijenata biljkama, regulišu njihov hormonski status kao i odnos prema biljnim patogenima. Bakterije i kvasci koji stimulišu rast biljaka igraju važnu ulogu i u procesima bioremedijacije. Trideset bakterijskih i deset izolata kvasaca je izolovano iz kontaminiranog zemljišta (PAH i PCB) i testirano na prisustvo mehanizama kojima se pospešuje rast biljaka. Cilj je bio doći do sojeva koji su prilagođeni na život u zagađenom zemljištu, a istovremeno imaju i potencijalno stimulativno dejstvo. Nakon izvršenih biohemijskih analiza (produkcija amonijaka, indol-sirćetne kiseline, siderofora, rastvaranje neorganskog fosfora) u stimulatore biljnog rasta svrstana su tri bakterijska (Serratia liquefaciens, Micrococcus sp. i Serratia sp.) i dva izolata kvasaca (Candida utilis i Candida tropicalis). Među njima najveću sposobnost produkcije indol-sirćetne kiseline je pokazao izolat Serratia sp. (25,5 μg/ml). Analize tolerancije na prisustvo teških metala (Cu+2, Cr+6 i Ni+2) pokazale su da su izolati Serratia liquefaciens, Micrococcus sp., Serratia sp. i Candida tropicalis sposobni da podnesu više koncentracije. Rezultati ovih istraživanja mogu imati praktičnu primenu u budućim remedijacionim aktivnostima i unapređenju kvaliteta životne sredine.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Isolation and characterization of bacteria and yeasts from contaminated soil
T1  - Izolacija i karakterizacija bakterija i kvasaca iz kontaminiranog zemljišta
EP  - 256
IS  - 3
SP  - 247
VL  - 61
DO  - 10.2298/JAS1603247K
ER  - 

@article{
author = "Karličić, Vera and Radić, Danka and Jovičić-Petrović, Jelena and Lalević, Blažo and Jovanović, Ljubinko and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "Plant growth promoting (PGP) bacteria and yeasts play an important role in bioremediation processes. Thirty bacterial and ten yeast isolates were obtained from PAH and PCB contaminated soil with an aim of determining the presence of PGP mechanisms (production of ammonia, indoleacetic acid, siderophores and solubilization of inorganic phosphate). As a result, three bacterial (Serratia liquefaciens, Micrococcus sp. and Serratia sp.) and two yeast isolates (Candida utilis and Candida tropicalis) were recognized as PGP strains. Among them, Serratia sp. showed the highest indole production (25.5 μg/ml). Analyses of metal tolerance (Cu+2, Cr+6 and Ni+2) revealed that Serratia liquefaciens, Micrococcus sp., Serratia sp. and Candida tropicalis were capable to tolerate significant concentration of metals. As a result of this study several bacterial and yeast strains were attributed as potential plant growth promoters which can be applied in future remediation activities and environmental quality improvements., Zemljište predstavlja dinamičan ekosistem naseljen brojnim mikroorganizmima među kojima su bakterije najbrojnije. Najveći broj je skoncentrisan u uskoj zoni zemljišta koja okružuje koren i koja se naziva rizosfera. Procenjuje se da 1-2% bakterija koje naseljavaju ovu zonu imaju sposobnost da promovišu rast biljaka (engl. plant growth promoting bacteria - PGPB). Pored bakterija, sve više je podataka da i zemljišni kvasci poseduju ove sposobnosti. Mikroorganizmi koji stimulišu rast biljaka koriste različite mehanizme kojima povećavaju dostupnost nutrijenata biljkama, regulišu njihov hormonski status kao i odnos prema biljnim patogenima. Bakterije i kvasci koji stimulišu rast biljaka igraju važnu ulogu i u procesima bioremedijacije. Trideset bakterijskih i deset izolata kvasaca je izolovano iz kontaminiranog zemljišta (PAH i PCB) i testirano na prisustvo mehanizama kojima se pospešuje rast biljaka. Cilj je bio doći do sojeva koji su prilagođeni na život u zagađenom zemljištu, a istovremeno imaju i potencijalno stimulativno dejstvo. Nakon izvršenih biohemijskih analiza (produkcija amonijaka, indol-sirćetne kiseline, siderofora, rastvaranje neorganskog fosfora) u stimulatore biljnog rasta svrstana su tri bakterijska (Serratia liquefaciens, Micrococcus sp. i Serratia sp.) i dva izolata kvasaca (Candida utilis i Candida tropicalis). Među njima najveću sposobnost produkcije indol-sirćetne kiseline je pokazao izolat Serratia sp. (25,5 μg/ml). Analize tolerancije na prisustvo teških metala (Cu+2, Cr+6 i Ni+2) pokazale su da su izolati Serratia liquefaciens, Micrococcus sp., Serratia sp. i Candida tropicalis sposobni da podnesu više koncentracije. Rezultati ovih istraživanja mogu imati praktičnu primenu u budućim remedijacionim aktivnostima i unapređenju kvaliteta životne sredine.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Isolation and characterization of bacteria and yeasts from contaminated soil, Izolacija i karakterizacija bakterija i kvasaca iz kontaminiranog zemljišta",
pages = "256-247",
number = "3",
volume = "61",
doi = "10.2298/JAS1603247K"
}

Karličić, V., Radić, D., Jovičić-Petrović, J., Lalević, B., Jovanović, L., Kiković, D.,& Raičević, V.. (2016). Isolation and characterization of bacteria and yeasts from contaminated soil. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 61(3), 247-256.
https://doi.org/10.2298/JAS1603247K

Karličić V, Radić D, Jovičić-Petrović J, Lalević B, Jovanović L, Kiković D, Raičević V. Isolation and characterization of bacteria and yeasts from contaminated soil. in Journal of Agricultural Sciences (Belgrade). 2016;61(3):247-256.
doi:10.2298/JAS1603247K .

Karličić, Vera, Radić, Danka, Jovičić-Petrović, Jelena, Lalević, Blažo, Jovanović, Ljubinko, Kiković, Dragan, Raičević, Vera, "Isolation and characterization of bacteria and yeasts from contaminated soil" in Journal of Agricultural Sciences (Belgrade), 61, no. 3 (2016):247-256,
https://doi.org/10.2298/JAS1603247K . .

TY  - JOUR
AU  - Karličić, Vera
AU  - Golubović-Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4307
AB  - Surface mining causes major destruction of natural landscapes and ecosystems. The most fertile, surface soil layer is lost permanently, together with vegetation, wildlife, and micro flora. Post-mining areas are characterized with diverse edaphic, topographic, hydrographic conditions, which complicate land restoration. Successful establishment of forest ecosystems on such land depends mostly on selection of tree species. The chosen plants must be capable of tolerating a wide range of acidity, fertility, moisture, and have potential to ameliorate such substrates for more demanding species. But, reforestation of heavily damaged ecosystems, such as post-mining areas, demands a new approach in seedlings production. This new approach takes into account specific requirements of habitat and integrates them into 'targeted production of planting material'. A good strategy for successful reforestation of post-mining areas is the input of organic matter (compost, mulch). Also, current knowledge and experiences emphasize the potential of beneficial microorganisms such as, mycorrhizal fungi (MF) and plant growth promoting rhizobacteria (PGPR). The majority of studies that deal with beneficial interactions between trees and microorganisms are focused on the mycorrhiza, while plant growth promoting rhizobacteria are less present in silviculture. In this study, the focus is on the reforestation challenges of two mining basins, Majdanpek and Kolubara and suggests beneficial microorganisms as potential solution. The study presents results of several years' researches on plant response to the presence of mycorrhizal fungi and PGPR. The substrates used for plant growth were Majdanpek and Kolubara mine deposals. Mycorrhizal seedlings were grown in Majdanpek mine deposal, and at the end of the experiment they had 30% higher biomass in comparison to control (seedlings without mycorrhiza). Seedlings linked with fungi had a higher survival rate. Deposals from Kolubara Mining Basin were used as a substrate for seedlings inoculated with PGPR. In the first experiment, Scots pine and Norway spruce were inoculated with Azotobacter chroococcum, Bacillus megaterium, B. circulans, B. licheniformis, B. pumilus, B. amyloliquefaciens. Inoculation resulted with higher biomass production (Scots pine 43%, Norway spruce 34%). Similar results were obtained in the second experiment where Scots pine and black locust were inoculated with Bacillus licheniformis, Aeromonas hydrophila, Pseudomonas putida and Burkholderia cepacia. Both species had higher biomass (around 20%) in comparison to un-inoculated control. The results confirmed the fact that early establishment and successful growth of vegetation on devastated areas depends on the presence and activity of soil microbes. Microorganisms as a 'nature's solution' pose the potential to alleviate reforestation challenges of anthropogenic devastated landscapes. Their presence and activity is crucial for ecosystem stability. In areas with compromised balance, their introduction is justified action for achieving the goal of long term ecosystem sustainability.
PB  - SPS Reforesta, Beograd
T2  - Reforesta
T1  - The alleviation of reforestation challenges by beneficial soil microorganisms
EP  - 260
IS  - 1
SP  - 238
VL  - 1
DO  - 10.21750/REFOR.1.12.12
ER  - 

@article{
author = "Karličić, Vera and Golubović-Ćurguz, Vesna and Raičević, Vera",
year = "2016",
abstract = "Surface mining causes major destruction of natural landscapes and ecosystems. The most fertile, surface soil layer is lost permanently, together with vegetation, wildlife, and micro flora. Post-mining areas are characterized with diverse edaphic, topographic, hydrographic conditions, which complicate land restoration. Successful establishment of forest ecosystems on such land depends mostly on selection of tree species. The chosen plants must be capable of tolerating a wide range of acidity, fertility, moisture, and have potential to ameliorate such substrates for more demanding species. But, reforestation of heavily damaged ecosystems, such as post-mining areas, demands a new approach in seedlings production. This new approach takes into account specific requirements of habitat and integrates them into 'targeted production of planting material'. A good strategy for successful reforestation of post-mining areas is the input of organic matter (compost, mulch). Also, current knowledge and experiences emphasize the potential of beneficial microorganisms such as, mycorrhizal fungi (MF) and plant growth promoting rhizobacteria (PGPR). The majority of studies that deal with beneficial interactions between trees and microorganisms are focused on the mycorrhiza, while plant growth promoting rhizobacteria are less present in silviculture. In this study, the focus is on the reforestation challenges of two mining basins, Majdanpek and Kolubara and suggests beneficial microorganisms as potential solution. The study presents results of several years' researches on plant response to the presence of mycorrhizal fungi and PGPR. The substrates used for plant growth were Majdanpek and Kolubara mine deposals. Mycorrhizal seedlings were grown in Majdanpek mine deposal, and at the end of the experiment they had 30% higher biomass in comparison to control (seedlings without mycorrhiza). Seedlings linked with fungi had a higher survival rate. Deposals from Kolubara Mining Basin were used as a substrate for seedlings inoculated with PGPR. In the first experiment, Scots pine and Norway spruce were inoculated with Azotobacter chroococcum, Bacillus megaterium, B. circulans, B. licheniformis, B. pumilus, B. amyloliquefaciens. Inoculation resulted with higher biomass production (Scots pine 43%, Norway spruce 34%). Similar results were obtained in the second experiment where Scots pine and black locust were inoculated with Bacillus licheniformis, Aeromonas hydrophila, Pseudomonas putida and Burkholderia cepacia. Both species had higher biomass (around 20%) in comparison to un-inoculated control. The results confirmed the fact that early establishment and successful growth of vegetation on devastated areas depends on the presence and activity of soil microbes. Microorganisms as a 'nature's solution' pose the potential to alleviate reforestation challenges of anthropogenic devastated landscapes. Their presence and activity is crucial for ecosystem stability. In areas with compromised balance, their introduction is justified action for achieving the goal of long term ecosystem sustainability.",
publisher = "SPS Reforesta, Beograd",
journal = "Reforesta",
title = "The alleviation of reforestation challenges by beneficial soil microorganisms",
pages = "260-238",
number = "1",
volume = "1",
doi = "10.21750/REFOR.1.12.12"
}

Karličić, V., Golubović-Ćurguz, V.,& Raičević, V.. (2016). The alleviation of reforestation challenges by beneficial soil microorganisms. in Reforesta
SPS Reforesta, Beograd., 1(1), 238-260.
https://doi.org/10.21750/REFOR.1.12.12

Karličić V, Golubović-Ćurguz V, Raičević V. The alleviation of reforestation challenges by beneficial soil microorganisms. in Reforesta. 2016;1(1):238-260.
doi:10.21750/REFOR.1.12.12 .

Karličić, Vera, Golubović-Ćurguz, Vesna, Raičević, Vera, "The alleviation of reforestation challenges by beneficial soil microorganisms" in Reforesta, 1, no. 1 (2016):238-260,
https://doi.org/10.21750/REFOR.1.12.12 . .