TY  - JOUR
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Jevremović, Sladjana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Subotić, Angelina
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2887
AB  - Viruses are responsible for numerous epidemics in different crops in all parts of the world. As a consequence of their presence great economic losses are being incurred. In addition to the development of sensitive techniques for detection, identification and characterization of viruses, substantial attention has also been paid to biotechnological methods for their elimination from plants. In this review article, the following biotechnological in vitro culture techniques for virus elimination from ornamental plants are presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or a combination of these methods. The plant species, as well as the type of virus determine the choice of a most suitable method. The state of the art in investigation of virus elimination from Impatiens sp. in Serbia is summarized.
AB  - Virusi su odgovorni za brojne epidemije na različitim usevima u svim delovima sveta. Posledica njihovog prisustva su velike ekonomske štete, pa osim razvoju osetljivih tehnika za detekciju, identifikaciju i karakterizaciju virusa, velika pažnja se poklanja i biotehnološkim metodama za njihovu eliminaciju. U ovom preglednom radu predstavljene su tehnike in vitro kulture za eliminaciju virusa iz biljnog materijala: kultura meristema, termoterapija, hemoterapija, krioterapija ili kombinacija ovih metoda. Koja će metoda biti primenjena zavisi od biljne vrste, kao i od vrste virusa. U radu je dat pregled istraživanja na eliminaciji virusa iz Impatiens sp. u Srbiji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Virus elimination from ornamental plants using in vitro culture techniques
T1  - Primena tehnika in vitro kulture za eliminaciju virusa iz ukrasnih biljaka
EP  - 211
IS  - 3
SP  - 203
VL  - 27
DO  - 10.2298/PIF1203203M
ER  - 

@article{
author = "Milošević, Snežana and Cingel, Aleksandar and Jevremović, Sladjana and Stanković, Ivana and Bulajić, Aleksandra and Krstić, Branka and Subotić, Angelina",
year = "2012",
abstract = "Viruses are responsible for numerous epidemics in different crops in all parts of the world. As a consequence of their presence great economic losses are being incurred. In addition to the development of sensitive techniques for detection, identification and characterization of viruses, substantial attention has also been paid to biotechnological methods for their elimination from plants. In this review article, the following biotechnological in vitro culture techniques for virus elimination from ornamental plants are presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or a combination of these methods. The plant species, as well as the type of virus determine the choice of a most suitable method. The state of the art in investigation of virus elimination from Impatiens sp. in Serbia is summarized., Virusi su odgovorni za brojne epidemije na različitim usevima u svim delovima sveta. Posledica njihovog prisustva su velike ekonomske štete, pa osim razvoju osetljivih tehnika za detekciju, identifikaciju i karakterizaciju virusa, velika pažnja se poklanja i biotehnološkim metodama za njihovu eliminaciju. U ovom preglednom radu predstavljene su tehnike in vitro kulture za eliminaciju virusa iz biljnog materijala: kultura meristema, termoterapija, hemoterapija, krioterapija ili kombinacija ovih metoda. Koja će metoda biti primenjena zavisi od biljne vrste, kao i od vrste virusa. U radu je dat pregled istraživanja na eliminaciji virusa iz Impatiens sp. u Srbiji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Virus elimination from ornamental plants using in vitro culture techniques, Primena tehnika in vitro kulture za eliminaciju virusa iz ukrasnih biljaka",
pages = "211-203",
number = "3",
volume = "27",
doi = "10.2298/PIF1203203M"
}

Milošević, S., Cingel, A., Jevremović, S., Stanković, I., Bulajić, A., Krstić, B.,& Subotić, A.. (2012). Virus elimination from ornamental plants using in vitro culture techniques. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(3), 203-211.
https://doi.org/10.2298/PIF1203203M

Milošević S, Cingel A, Jevremović S, Stanković I, Bulajić A, Krstić B, Subotić A. Virus elimination from ornamental plants using in vitro culture techniques. in Pesticidi i fitomedicina. 2012;27(3):203-211.
doi:10.2298/PIF1203203M .

Milošević, Snežana, Cingel, Aleksandar, Jevremović, Sladjana, Stanković, Ivana, Bulajić, Aleksandra, Krstić, Branka, Subotić, Angelina, "Virus elimination from ornamental plants using in vitro culture techniques" in Pesticidi i fitomedicina, 27, no. 3 (2012):203-211,
https://doi.org/10.2298/PIF1203203M . .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Subotić, Angelina
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Jevremović, Sladjana
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2667
AB  - The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.
PB  - Univ Catolica De Valparaiso, Valparaiso
T2  - Electronic Journal of Biotechnology
T1  - Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
IS  - 1
VL  - 14
DO  - 10.2225/vol14-issue1-fulltext-5
ER  - 

@article{
author = "Milošević, Snežana and Subotić, Angelina and Bulajić, Aleksandra and Djekić, Ivana and Jevremović, Sladjana and Vučurović, Ana and Krstić, Branka",
year = "2011",
abstract = "The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.",
publisher = "Univ Catolica De Valparaiso, Valparaiso",
journal = "Electronic Journal of Biotechnology",
title = "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant",
number = "1",
volume = "14",
doi = "10.2225/vol14-issue1-fulltext-5"
}

Milošević, S., Subotić, A., Bulajić, A., Djekić, I., Jevremović, S., Vučurović, A.,& Krstić, B.. (2011). Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology
Univ Catolica De Valparaiso, Valparaiso., 14(1).
https://doi.org/10.2225/vol14-issue1-fulltext-5

Milošević S, Subotić A, Bulajić A, Djekić I, Jevremović S, Vučurović A, Krstić B. Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology. 2011;14(1).
doi:10.2225/vol14-issue1-fulltext-5 .

Milošević, Snežana, Subotić, Angelina, Bulajić, Aleksandra, Djekić, Ivana, Jevremović, Sladjana, Vučurović, Ana, Krstić, Branka, "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant" in Electronic Journal of Biotechnology, 14, no. 1 (2011),
https://doi.org/10.2225/vol14-issue1-fulltext-5 . .