TY  - JOUR
AU  - Djurić, M.
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, M.
AU  - Cingel, Aleksandar
AU  - Vujičić, M.
AU  - Milošević, S.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5418
AB  - This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.
PB  - MDPI AG
T2  - Plants
T1  - Morpho-physiological and molecular evaluation of drought and recovery in impatiens walleriana grown ex vitro
EP  - 22
IS  - 11
SP  - 1
VL  - 9
DO  - 10.3390/plants9111559
ER  - 

@article{
author = "Djurić, M. and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, M. and Cingel, Aleksandar and Vujičić, M. and Milošević, S.",
year = "2020",
abstract = "This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.",
publisher = "MDPI AG",
journal = "Plants",
title = "Morpho-physiological and molecular evaluation of drought and recovery in impatiens walleriana grown ex vitro",
pages = "22-1",
number = "11",
volume = "9",
doi = "10.3390/plants9111559"
}

Djurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Vujičić, M.,& Milošević, S.. (2020). Morpho-physiological and molecular evaluation of drought and recovery in impatiens walleriana grown ex vitro. in Plants
MDPI AG., 9(11), 1-22.
https://doi.org/10.3390/plants9111559

Djurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Vujičić M, Milošević S. Morpho-physiological and molecular evaluation of drought and recovery in impatiens walleriana grown ex vitro. in Plants. 2020;9(11):1-22.
doi:10.3390/plants9111559 .

Djurić, M., Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, M., Cingel, Aleksandar, Vujičić, M., Milošević, S., "Morpho-physiological and molecular evaluation of drought and recovery in impatiens walleriana grown ex vitro" in Plants, 9, no. 11 (2020):1-22,
https://doi.org/10.3390/plants9111559 . .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Jevremović, Sladjana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Subotić, Angelina
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2887
AB  - Viruses are responsible for numerous epidemics in different crops in all parts of the world. As a consequence of their presence great economic losses are being incurred. In addition to the development of sensitive techniques for detection, identification and characterization of viruses, substantial attention has also been paid to biotechnological methods for their elimination from plants. In this review article, the following biotechnological in vitro culture techniques for virus elimination from ornamental plants are presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or a combination of these methods. The plant species, as well as the type of virus determine the choice of a most suitable method. The state of the art in investigation of virus elimination from Impatiens sp. in Serbia is summarized.
AB  - Virusi su odgovorni za brojne epidemije na različitim usevima u svim delovima sveta. Posledica njihovog prisustva su velike ekonomske štete, pa osim razvoju osetljivih tehnika za detekciju, identifikaciju i karakterizaciju virusa, velika pažnja se poklanja i biotehnološkim metodama za njihovu eliminaciju. U ovom preglednom radu predstavljene su tehnike in vitro kulture za eliminaciju virusa iz biljnog materijala: kultura meristema, termoterapija, hemoterapija, krioterapija ili kombinacija ovih metoda. Koja će metoda biti primenjena zavisi od biljne vrste, kao i od vrste virusa. U radu je dat pregled istraživanja na eliminaciji virusa iz Impatiens sp. u Srbiji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Virus elimination from ornamental plants using in vitro culture techniques
T1  - Primena tehnika in vitro kulture za eliminaciju virusa iz ukrasnih biljaka
EP  - 211
IS  - 3
SP  - 203
VL  - 27
DO  - 10.2298/PIF1203203M
ER  - 

@article{
author = "Milošević, Snežana and Cingel, Aleksandar and Jevremović, Sladjana and Stanković, Ivana and Bulajić, Aleksandra and Krstić, Branka and Subotić, Angelina",
year = "2012",
abstract = "Viruses are responsible for numerous epidemics in different crops in all parts of the world. As a consequence of their presence great economic losses are being incurred. In addition to the development of sensitive techniques for detection, identification and characterization of viruses, substantial attention has also been paid to biotechnological methods for their elimination from plants. In this review article, the following biotechnological in vitro culture techniques for virus elimination from ornamental plants are presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or a combination of these methods. The plant species, as well as the type of virus determine the choice of a most suitable method. The state of the art in investigation of virus elimination from Impatiens sp. in Serbia is summarized., Virusi su odgovorni za brojne epidemije na različitim usevima u svim delovima sveta. Posledica njihovog prisustva su velike ekonomske štete, pa osim razvoju osetljivih tehnika za detekciju, identifikaciju i karakterizaciju virusa, velika pažnja se poklanja i biotehnološkim metodama za njihovu eliminaciju. U ovom preglednom radu predstavljene su tehnike in vitro kulture za eliminaciju virusa iz biljnog materijala: kultura meristema, termoterapija, hemoterapija, krioterapija ili kombinacija ovih metoda. Koja će metoda biti primenjena zavisi od biljne vrste, kao i od vrste virusa. U radu je dat pregled istraživanja na eliminaciji virusa iz Impatiens sp. u Srbiji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Virus elimination from ornamental plants using in vitro culture techniques, Primena tehnika in vitro kulture za eliminaciju virusa iz ukrasnih biljaka",
pages = "211-203",
number = "3",
volume = "27",
doi = "10.2298/PIF1203203M"
}

Milošević, S., Cingel, A., Jevremović, S., Stanković, I., Bulajić, A., Krstić, B.,& Subotić, A.. (2012). Virus elimination from ornamental plants using in vitro culture techniques. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(3), 203-211.
https://doi.org/10.2298/PIF1203203M

Milošević S, Cingel A, Jevremović S, Stanković I, Bulajić A, Krstić B, Subotić A. Virus elimination from ornamental plants using in vitro culture techniques. in Pesticidi i fitomedicina. 2012;27(3):203-211.
doi:10.2298/PIF1203203M .

Milošević, Snežana, Cingel, Aleksandar, Jevremović, Sladjana, Stanković, Ivana, Bulajić, Aleksandra, Krstić, Branka, Subotić, Angelina, "Virus elimination from ornamental plants using in vitro culture techniques" in Pesticidi i fitomedicina, 27, no. 3 (2012):203-211,
https://doi.org/10.2298/PIF1203203M . .