TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6384
AB  - In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level. © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.
T2  - Plant Signaling and Behavior
T2  - Plant Signaling and Behavior
T1  - Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress
IS  - 1
VL  - 18
DO  - 10.1080/15592324.2023.2219936
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level. © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.",
journal = "Plant Signaling and Behavior, Plant Signaling and Behavior",
title = "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress",
number = "1",
volume = "18",
doi = "10.1080/15592324.2023.2219936"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling and Behavior, 18(1).
https://doi.org/10.1080/15592324.2023.2219936

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling and Behavior. 2023;18(1).
doi:10.1080/15592324.2023.2219936 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress" in Plant Signaling and Behavior, 18, no. 1 (2023),
https://doi.org/10.1080/15592324.2023.2219936 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6363
AB  - Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general. © 2023 by the authors.
T2  - Genes
T2  - Genes
T1  - Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application
IS  - 5
VL  - 14
DO  - 10.3390/genes14051072
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general. © 2023 by the authors.",
journal = "Genes, Genes",
title = "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application",
number = "5",
volume = "14",
doi = "10.3390/genes14051072"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes, 14(5).
https://doi.org/10.3390/genes14051072

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes. 2023;14(5).
doi:10.3390/genes14051072 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application" in Genes, 14, no. 5 (2023),
https://doi.org/10.3390/genes14051072 . .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Jevremović, Sladjana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Subotić, Angelina
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2887
AB  - Viruses are responsible for numerous epidemics in different crops in all parts of the world. As a consequence of their presence great economic losses are being incurred. In addition to the development of sensitive techniques for detection, identification and characterization of viruses, substantial attention has also been paid to biotechnological methods for their elimination from plants. In this review article, the following biotechnological in vitro culture techniques for virus elimination from ornamental plants are presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or a combination of these methods. The plant species, as well as the type of virus determine the choice of a most suitable method. The state of the art in investigation of virus elimination from Impatiens sp. in Serbia is summarized.
AB  - Virusi su odgovorni za brojne epidemije na različitim usevima u svim delovima sveta. Posledica njihovog prisustva su velike ekonomske štete, pa osim razvoju osetljivih tehnika za detekciju, identifikaciju i karakterizaciju virusa, velika pažnja se poklanja i biotehnološkim metodama za njihovu eliminaciju. U ovom preglednom radu predstavljene su tehnike in vitro kulture za eliminaciju virusa iz biljnog materijala: kultura meristema, termoterapija, hemoterapija, krioterapija ili kombinacija ovih metoda. Koja će metoda biti primenjena zavisi od biljne vrste, kao i od vrste virusa. U radu je dat pregled istraživanja na eliminaciji virusa iz Impatiens sp. u Srbiji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Virus elimination from ornamental plants using in vitro culture techniques
T1  - Primena tehnika in vitro kulture za eliminaciju virusa iz ukrasnih biljaka
EP  - 211
IS  - 3
SP  - 203
VL  - 27
DO  - 10.2298/PIF1203203M
ER  - 

@article{
author = "Milošević, Snežana and Cingel, Aleksandar and Jevremović, Sladjana and Stanković, Ivana and Bulajić, Aleksandra and Krstić, Branka and Subotić, Angelina",
year = "2012",
abstract = "Viruses are responsible for numerous epidemics in different crops in all parts of the world. As a consequence of their presence great economic losses are being incurred. In addition to the development of sensitive techniques for detection, identification and characterization of viruses, substantial attention has also been paid to biotechnological methods for their elimination from plants. In this review article, the following biotechnological in vitro culture techniques for virus elimination from ornamental plants are presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or a combination of these methods. The plant species, as well as the type of virus determine the choice of a most suitable method. The state of the art in investigation of virus elimination from Impatiens sp. in Serbia is summarized., Virusi su odgovorni za brojne epidemije na različitim usevima u svim delovima sveta. Posledica njihovog prisustva su velike ekonomske štete, pa osim razvoju osetljivih tehnika za detekciju, identifikaciju i karakterizaciju virusa, velika pažnja se poklanja i biotehnološkim metodama za njihovu eliminaciju. U ovom preglednom radu predstavljene su tehnike in vitro kulture za eliminaciju virusa iz biljnog materijala: kultura meristema, termoterapija, hemoterapija, krioterapija ili kombinacija ovih metoda. Koja će metoda biti primenjena zavisi od biljne vrste, kao i od vrste virusa. U radu je dat pregled istraživanja na eliminaciji virusa iz Impatiens sp. u Srbiji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Virus elimination from ornamental plants using in vitro culture techniques, Primena tehnika in vitro kulture za eliminaciju virusa iz ukrasnih biljaka",
pages = "211-203",
number = "3",
volume = "27",
doi = "10.2298/PIF1203203M"
}

Milošević, S., Cingel, A., Jevremović, S., Stanković, I., Bulajić, A., Krstić, B.,& Subotić, A.. (2012). Virus elimination from ornamental plants using in vitro culture techniques. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(3), 203-211.
https://doi.org/10.2298/PIF1203203M

Milošević S, Cingel A, Jevremović S, Stanković I, Bulajić A, Krstić B, Subotić A. Virus elimination from ornamental plants using in vitro culture techniques. in Pesticidi i fitomedicina. 2012;27(3):203-211.
doi:10.2298/PIF1203203M .

Milošević, Snežana, Cingel, Aleksandar, Jevremović, Sladjana, Stanković, Ivana, Bulajić, Aleksandra, Krstić, Branka, Subotić, Angelina, "Virus elimination from ornamental plants using in vitro culture techniques" in Pesticidi i fitomedicina, 27, no. 3 (2012):203-211,
https://doi.org/10.2298/PIF1203203M . .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Subotić, Angelina
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Jevremović, Sladjana
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2667
AB  - The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.
PB  - Univ Catolica De Valparaiso, Valparaiso
T2  - Electronic Journal of Biotechnology
T1  - Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
IS  - 1
VL  - 14
DO  - 10.2225/vol14-issue1-fulltext-5
ER  - 

@article{
author = "Milošević, Snežana and Subotić, Angelina and Bulajić, Aleksandra and Djekić, Ivana and Jevremović, Sladjana and Vučurović, Ana and Krstić, Branka",
year = "2011",
abstract = "The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.",
publisher = "Univ Catolica De Valparaiso, Valparaiso",
journal = "Electronic Journal of Biotechnology",
title = "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant",
number = "1",
volume = "14",
doi = "10.2225/vol14-issue1-fulltext-5"
}

Milošević, S., Subotić, A., Bulajić, A., Djekić, I., Jevremović, S., Vučurović, A.,& Krstić, B.. (2011). Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology
Univ Catolica De Valparaiso, Valparaiso., 14(1).
https://doi.org/10.2225/vol14-issue1-fulltext-5

Milošević S, Subotić A, Bulajić A, Djekić I, Jevremović S, Vučurović A, Krstić B. Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology. 2011;14(1).
doi:10.2225/vol14-issue1-fulltext-5 .

Milošević, Snežana, Subotić, Angelina, Bulajić, Aleksandra, Djekić, Ivana, Jevremović, Sladjana, Vučurović, Ana, Krstić, Branka, "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant" in Electronic Journal of Biotechnology, 14, no. 1 (2011),
https://doi.org/10.2225/vol14-issue1-fulltext-5 . .