TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Šoštarić, Ivan
AU  - Jevdjović, Radosav
AU  - Zivković, Jelena
AU  - Razić, Slavica
AU  - Moravčević, Djordje
AU  - Maksimović, Zoran
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5039
AB  - In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.
EP  - 169
SP  - 162
VL  - 130
DO  - 10.1016/j.indcrop.2018.12.055
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Šoštarić, Ivan and Jevdjović, Radosav and Zivković, Jelena and Razić, Slavica and Moravčević, Djordje and Maksimović, Zoran",
year = "2019",
abstract = "In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.",
pages = "169-162",
volume = "130",
doi = "10.1016/j.indcrop.2018.12.055"
}

Arsenijević, J., Drobac, M., Šoštarić, I., Jevdjović, R., Zivković, J., Razić, S., Moravčević, D.,& Maksimović, Z.. (2019). Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 130, 162-169.
https://doi.org/10.1016/j.indcrop.2018.12.055

Arsenijević J, Drobac M, Šoštarić I, Jevdjović R, Zivković J, Razić S, Moravčević D, Maksimović Z. Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products. 2019;130:162-169.
doi:10.1016/j.indcrop.2018.12.055 .

Arsenijević, Jelena, Drobac, Milica, Šoštarić, Ivan, Jevdjović, Radosav, Zivković, Jelena, Razić, Slavica, Moravčević, Djordje, Maksimović, Zoran, "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All." in Industrial Crops and Products, 130 (2019):162-169,
https://doi.org/10.1016/j.indcrop.2018.12.055 . .

TY  - JOUR
AU  - Veljković, Bojana
AU  - Šoštarić, Ivan
AU  - Dajić-Stevanović, Zora
AU  - Liber, Zlatko
AU  - Satović, Zlatko
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4953
AB  - The red raspberry (Rubus idaeas L.) is one of the most important fruit species in Serbia; there are currently approximately 11000 ha under cultivation. Wild red raspberry populations can be an important source of genes for breeding new raspberry varieties. This study was carried out to determine the genetic variability of wild populations, as well as their relationship with the most common cultivated cultivars using amplified fragment length polymorphism (AFLP) markers. In this study were included 128 individuals from seven wild populations and seven cultivar specimens from a nursery ('Mekeer', 'Willamette', 'Polka', 'Polana', 'Loganberry', Tayberry, 'Black Jewel'). Four AFLP combinations yielded a total of 247 polymorphic bands in 135 R. idaeus specimens. Analysis of AFLP markers showed that the cultivars are dearly distinct from wild populations. Population P4 (Mt. Ozren) had the highest gene diversity, the highest ntunber of private markers and the highest rarity index. Analysis of molecular variance among and within wild populations showed that the most of the genetic diversity was attributable to differences between individuals within populations. A Mantel test showed that differences in altitude play the crucial role in structuring of the genetic diversity of red raspberry populations. Altitude contributed more to the population differentiation than spatial distance. Populations of wild raspberry from the territory of Serbia are characterized by high diversity, and should be protected and used as a resource in selection and breeding processes for the production of new varieties, with improved properties in terms of better yield and stronger resistance to external factors.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Genetic structure of wild raspberry populations in the Central Balkans depends on their location and on their relationship to commercial cultivars
VL  - 256
DO  - 10.1016/j.scienta.2019.108606
ER  - 

@article{
author = "Veljković, Bojana and Šoštarić, Ivan and Dajić-Stevanović, Zora and Liber, Zlatko and Satović, Zlatko",
year = "2019",
abstract = "The red raspberry (Rubus idaeas L.) is one of the most important fruit species in Serbia; there are currently approximately 11000 ha under cultivation. Wild red raspberry populations can be an important source of genes for breeding new raspberry varieties. This study was carried out to determine the genetic variability of wild populations, as well as their relationship with the most common cultivated cultivars using amplified fragment length polymorphism (AFLP) markers. In this study were included 128 individuals from seven wild populations and seven cultivar specimens from a nursery ('Mekeer', 'Willamette', 'Polka', 'Polana', 'Loganberry', Tayberry, 'Black Jewel'). Four AFLP combinations yielded a total of 247 polymorphic bands in 135 R. idaeus specimens. Analysis of AFLP markers showed that the cultivars are dearly distinct from wild populations. Population P4 (Mt. Ozren) had the highest gene diversity, the highest ntunber of private markers and the highest rarity index. Analysis of molecular variance among and within wild populations showed that the most of the genetic diversity was attributable to differences between individuals within populations. A Mantel test showed that differences in altitude play the crucial role in structuring of the genetic diversity of red raspberry populations. Altitude contributed more to the population differentiation than spatial distance. Populations of wild raspberry from the territory of Serbia are characterized by high diversity, and should be protected and used as a resource in selection and breeding processes for the production of new varieties, with improved properties in terms of better yield and stronger resistance to external factors.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Genetic structure of wild raspberry populations in the Central Balkans depends on their location and on their relationship to commercial cultivars",
volume = "256",
doi = "10.1016/j.scienta.2019.108606"
}

Veljković, B., Šoštarić, I., Dajić-Stevanović, Z., Liber, Z.,& Satović, Z.. (2019). Genetic structure of wild raspberry populations in the Central Balkans depends on their location and on their relationship to commercial cultivars. in Scientia Horticulturae
Elsevier, Amsterdam., 256.
https://doi.org/10.1016/j.scienta.2019.108606

Veljković B, Šoštarić I, Dajić-Stevanović Z, Liber Z, Satović Z. Genetic structure of wild raspberry populations in the Central Balkans depends on their location and on their relationship to commercial cultivars. in Scientia Horticulturae. 2019;256.
doi:10.1016/j.scienta.2019.108606 .

Veljković, Bojana, Šoštarić, Ivan, Dajić-Stevanović, Zora, Liber, Zlatko, Satović, Zlatko, "Genetic structure of wild raspberry populations in the Central Balkans depends on their location and on their relationship to commercial cultivars" in Scientia Horticulturae, 256 (2019),
https://doi.org/10.1016/j.scienta.2019.108606 . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Godjevac, Dejan
AU  - Tešević, Vele
AU  - Dajić-Stevanović, Zora
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Novaković, Miroslav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5049
AB  - A new flavonoid glucoside derivative, patuletin 3-O-(2-O-feruloyl)-beta-D-glucuronopyranosyl-(1 -> 2)-beta-D-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, beta-D-glucopyranosyl-3-O-(2-O-sulfo-beta-D-galactopyranosyl)-(1 -> 2)-alpha-L-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, beta-D-glucopyranosyl-3-O-(2-O-sulfo-beta-D-galactopyranosyl)-(1 -> 2)-alpha-L-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and beta-D-glucopyranosyl-3-D-(beta-D-glucopyranosyl-(1 -> 2)-beta-D-galactopyranosyl-(1 -> 2)-alpha-L-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3-O-beta-D-apiofuranosyl-(1 '''-> 2 '')-beta-D-glucopyranoside (5) and patuletin 3-O-5 '''-O-feruloyl-beta-D-apiofuranosyl-(1 '''-> 2 '')-beta-D-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Natural Products
T1  - Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa
EP  - 1495
IS  - 6
SP  - 1487
VL  - 82
DO  - 10.1021/acs.jnatprod.8b00970
ER  - 

@article{
author = "Stanković, Jovana and Godjevac, Dejan and Tešević, Vele and Dajić-Stevanović, Zora and Ćirić, Ana and Soković, Marina and Novaković, Miroslav",
year = "2019",
abstract = "A new flavonoid glucoside derivative, patuletin 3-O-(2-O-feruloyl)-beta-D-glucuronopyranosyl-(1 -> 2)-beta-D-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, beta-D-glucopyranosyl-3-O-(2-O-sulfo-beta-D-galactopyranosyl)-(1 -> 2)-alpha-L-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, beta-D-glucopyranosyl-3-O-(2-O-sulfo-beta-D-galactopyranosyl)-(1 -> 2)-alpha-L-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and beta-D-glucopyranosyl-3-D-(beta-D-glucopyranosyl-(1 -> 2)-beta-D-galactopyranosyl-(1 -> 2)-alpha-L-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3-O-beta-D-apiofuranosyl-(1 '''-> 2 '')-beta-D-glucopyranoside (5) and patuletin 3-O-5 '''-O-feruloyl-beta-D-apiofuranosyl-(1 '''-> 2 '')-beta-D-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Natural Products",
title = "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa",
pages = "1495-1487",
number = "6",
volume = "82",
doi = "10.1021/acs.jnatprod.8b00970"
}

Stanković, J., Godjevac, D., Tešević, V., Dajić-Stevanović, Z., Ćirić, A., Soković, M.,& Novaković, M.. (2019). Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa. in Journal of Natural Products
Amer Chemical Soc, Washington., 82(6), 1487-1495.
https://doi.org/10.1021/acs.jnatprod.8b00970

Stanković J, Godjevac D, Tešević V, Dajić-Stevanović Z, Ćirić A, Soković M, Novaković M. Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa. in Journal of Natural Products. 2019;82(6):1487-1495.
doi:10.1021/acs.jnatprod.8b00970 .

Stanković, Jovana, Godjevac, Dejan, Tešević, Vele, Dajić-Stevanović, Zora, Ćirić, Ana, Soković, Marina, Novaković, Miroslav, "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa" in Journal of Natural Products, 82, no. 6 (2019):1487-1495,
https://doi.org/10.1021/acs.jnatprod.8b00970 . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Zdunić, Gordana
AU  - Dajić-Stevanović, Zora
AU  - Godjevac, Dejan
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4980
AB  - This study was performed to determine the main antibacterial compounds of the essential oil (EO) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC-MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil
EP  - 1365
IS  - 12
SP  - 1355
VL  - 84
DO  - 10.2298/JSC190513106S
ER  - 

@article{
author = "Stanković, Jovana and Novaković, Miroslav and Tešević, Vele and Ćirić, Ana and Soković, Marina and Zdunić, Gordana and Dajić-Stevanović, Zora and Godjevac, Dejan",
year = "2019",
abstract = "This study was performed to determine the main antibacterial compounds of the essential oil (EO) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC-MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil",
pages = "1365-1355",
number = "12",
volume = "84",
doi = "10.2298/JSC190513106S"
}

Stanković, J., Novaković, M., Tešević, V., Ćirić, A., Soković, M., Zdunić, G., Dajić-Stevanović, Z.,& Godjevac, D.. (2019). HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 84(12), 1355-1365.
https://doi.org/10.2298/JSC190513106S

Stanković J, Novaković M, Tešević V, Ćirić A, Soković M, Zdunić G, Dajić-Stevanović Z, Godjevac D. HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2019;84(12):1355-1365.
doi:10.2298/JSC190513106S .

Stanković, Jovana, Novaković, Miroslav, Tešević, Vele, Ćirić, Ana, Soković, Marina, Zdunić, Gordana, Dajić-Stevanović, Zora, Godjevac, Dejan, "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 84, no. 12 (2019):1355-1365,
https://doi.org/10.2298/JSC190513106S . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Bosnjak-Neumuller, Jasna
AU  - Pajić-Lijaković, Ivana
AU  - Raj, Jog
AU  - Vasiljević, Marko
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4631
AB  - The inconsistency of phytogenic feed additives' (PFA) effects on the livestock industry poses a risk for their use as a replacement for antibiotic growth promoters. The livestock market is being encouraged to use natural growth promotors, but information is limited about the PFA mode of action. The aim of this paper is to present the complexity of compounds present in essential oils (EOs) and factors that influence biological effects of PFA. In this paper, we highlight various controls and optimization parameters that influence the processes for the standardization of these products. The chemical composition of EOs depends on plant genetics, growth conditions, development stage at harvest, and processes of extracting active compounds. Their biological effects are further influenced by the interaction of phytochemicals and their bioavailability in the gastrointestinal tract of animals. PFA effects on animal health and production are also complex due to various EO antibiotic, antioxidant, anti-quorum sensing, anti-inflammatory, and digestive fluids stimulating activities. Research must focus on reliable methods to identify and control the quality and effects of EOs. In this study, we focused on available microencapsulation techniques of EOs to increase the bioavailability of active compounds, as well as their application in the animal feed additive industry.
PB  - MDPI, BASEL
T2  - Molecules
T1  - Essential Oils as Feed AdditivesFuture Perspectives
IS  - 7
VL  - 23
DO  - 10.3390/molecules23071717
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Bosnjak-Neumuller, Jasna and Pajić-Lijaković, Ivana and Raj, Jog and Vasiljević, Marko",
year = "2018",
abstract = "The inconsistency of phytogenic feed additives' (PFA) effects on the livestock industry poses a risk for their use as a replacement for antibiotic growth promoters. The livestock market is being encouraged to use natural growth promotors, but information is limited about the PFA mode of action. The aim of this paper is to present the complexity of compounds present in essential oils (EOs) and factors that influence biological effects of PFA. In this paper, we highlight various controls and optimization parameters that influence the processes for the standardization of these products. The chemical composition of EOs depends on plant genetics, growth conditions, development stage at harvest, and processes of extracting active compounds. Their biological effects are further influenced by the interaction of phytochemicals and their bioavailability in the gastrointestinal tract of animals. PFA effects on animal health and production are also complex due to various EO antibiotic, antioxidant, anti-quorum sensing, anti-inflammatory, and digestive fluids stimulating activities. Research must focus on reliable methods to identify and control the quality and effects of EOs. In this study, we focused on available microencapsulation techniques of EOs to increase the bioavailability of active compounds, as well as their application in the animal feed additive industry.",
publisher = "MDPI, BASEL",
journal = "Molecules",
title = "Essential Oils as Feed AdditivesFuture Perspectives",
number = "7",
volume = "23",
doi = "10.3390/molecules23071717"
}

Dajić-Stevanović, Z., Bosnjak-Neumuller, J., Pajić-Lijaković, I., Raj, J.,& Vasiljević, M.. (2018). Essential Oils as Feed AdditivesFuture Perspectives. in Molecules
MDPI, BASEL., 23(7).
https://doi.org/10.3390/molecules23071717

Dajić-Stevanović Z, Bosnjak-Neumuller J, Pajić-Lijaković I, Raj J, Vasiljević M. Essential Oils as Feed AdditivesFuture Perspectives. in Molecules. 2018;23(7).
doi:10.3390/molecules23071717 .

Dajić-Stevanović, Zora, Bosnjak-Neumuller, Jasna, Pajić-Lijaković, Ivana, Raj, Jog, Vasiljević, Marko, "Essential Oils as Feed AdditivesFuture Perspectives" in Molecules, 23, no. 7 (2018),
https://doi.org/10.3390/molecules23071717 . .

TY  - JOUR
AU  - Bosnjak-Neumuller, Jasna
AU  - Rančić, Dragana
AU  - Pećinar, Ilinka
AU  - Djelić, Ninoslav
AU  - Dajić-Stevanović, Zora
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4810
AB  - Micromorphological and anatomical analyses of the indumentum of Nepeta rtanjensis, a Serbian/local endemic species, were performed by means of light and electron microscopy and described in detail. The leaves and stems bear numerous glandular trichomes, both peltate and capitate, as well as non-glandular unbranched trichomes. A basal epidermal cell, a short stalk cell and a large round head of four secretory cells constitute one peltate trichome. Capitate trichomes are short and long-stalked and can be divided into two types, based on the number of glandular head cells, and further into a few subtypes according to the length and number of cells in stalk. Capitate trichomes of smaller length with one-cellular or with a bicellular head have been previously reported in different Nepeta species; however, according to our best knowledge, no existing study has reported the presence of long capitate trichomes on vegetative organs in any other species of this genus.
PB  - Pakistan Botanical Society
T2  - Pakistan Journal of Botany
T1  - Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (lamiaceae)
EP  - 269
IS  - 1
SP  - 259
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4810
ER  - 

@article{
author = "Bosnjak-Neumuller, Jasna and Rančić, Dragana and Pećinar, Ilinka and Djelić, Ninoslav and Dajić-Stevanović, Zora",
year = "2018",
abstract = "Micromorphological and anatomical analyses of the indumentum of Nepeta rtanjensis, a Serbian/local endemic species, were performed by means of light and electron microscopy and described in detail. The leaves and stems bear numerous glandular trichomes, both peltate and capitate, as well as non-glandular unbranched trichomes. A basal epidermal cell, a short stalk cell and a large round head of four secretory cells constitute one peltate trichome. Capitate trichomes are short and long-stalked and can be divided into two types, based on the number of glandular head cells, and further into a few subtypes according to the length and number of cells in stalk. Capitate trichomes of smaller length with one-cellular or with a bicellular head have been previously reported in different Nepeta species; however, according to our best knowledge, no existing study has reported the presence of long capitate trichomes on vegetative organs in any other species of this genus.",
publisher = "Pakistan Botanical Society",
journal = "Pakistan Journal of Botany",
title = "Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (lamiaceae)",
pages = "269-259",
number = "1",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4810"
}

Bosnjak-Neumuller, J., Rančić, D., Pećinar, I., Djelić, N.,& Dajić-Stevanović, Z.. (2018). Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (lamiaceae). in Pakistan Journal of Botany
Pakistan Botanical Society., 50(1), 259-269.
https://hdl.handle.net/21.15107/rcub_agrospace_4810

Bosnjak-Neumuller J, Rančić D, Pećinar I, Djelić N, Dajić-Stevanović Z. Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (lamiaceae). in Pakistan Journal of Botany. 2018;50(1):259-269.
https://hdl.handle.net/21.15107/rcub_agrospace_4810 .

Bosnjak-Neumuller, Jasna, Rančić, Dragana, Pećinar, Ilinka, Djelić, Ninoslav, Dajić-Stevanović, Zora, "Micromorphology and histochemistry of trichomes of endemic nepeta rtanjensis (lamiaceae)" in Pakistan Journal of Botany, 50, no. 1 (2018):259-269,
https://hdl.handle.net/21.15107/rcub_agrospace_4810 .

TY  - JOUR
AU  - Cebović, Tatjana
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Zivković, Jelena
AU  - Šoštarić, Ivan
AU  - Maksimović, Zoran
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4740
AB  - Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent
EP  - 567
IS  - 2
SP  - 560
VL  - 55
DO  - 10.1007/s13197-017-2965-1
ER  - 

@article{
author = "Cebović, Tatjana and Arsenijević, Jelena and Drobac, Milica and Zivković, Jelena and Šoštarić, Ivan and Maksimović, Zoran",
year = "2018",
abstract = "Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent",
pages = "567-560",
number = "2",
volume = "55",
doi = "10.1007/s13197-017-2965-1"
}

Cebović, T., Arsenijević, J., Drobac, M., Zivković, J., Šoštarić, I.,& Maksimović, Z.. (2018). Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 55(2), 560-567.
https://doi.org/10.1007/s13197-017-2965-1

Cebović T, Arsenijević J, Drobac M, Zivković J, Šoštarić I, Maksimović Z. Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore. 2018;55(2):560-567.
doi:10.1007/s13197-017-2965-1 .

Cebović, Tatjana, Arsenijević, Jelena, Drobac, Milica, Zivković, Jelena, Šoštarić, Ivan, Maksimović, Zoran, "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent" in Journal of Food Science and Technology-Mysore, 55, no. 2 (2018):560-567,
https://doi.org/10.1007/s13197-017-2965-1 . .

TY  - JOUR
AU  - Anicić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Pećinar, Ilinka
AU  - Brkusanin, Miloš
AU  - Nestorović-Zivković, Jasmina
AU  - Dmitrović, Slavica
AU  - Dajić-Stevanović, Zora
AU  - Schulz, Hartwig
AU  - Misić, Danijela
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4731
AB  - Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklic & Milojevic, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrISI. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants
EP  - 358
SP  - 347
VL  - 117
DO  - 10.1016/j.indcrop.2018.03.019
ER  - 

@article{
author = "Anicić, Neda and Matekalo, Dragana and Skorić, Marijana and Pećinar, Ilinka and Brkusanin, Miloš and Nestorović-Zivković, Jasmina and Dmitrović, Slavica and Dajić-Stevanović, Zora and Schulz, Hartwig and Misić, Danijela",
year = "2018",
abstract = "Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklic & Milojevic, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrISI. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants",
pages = "358-347",
volume = "117",
doi = "10.1016/j.indcrop.2018.03.019"
}

Anicić, N., Matekalo, D., Skorić, M., Pećinar, I., Brkusanin, M., Nestorović-Zivković, J., Dmitrović, S., Dajić-Stevanović, Z., Schulz, H.,& Misić, D.. (2018). Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 117, 347-358.
https://doi.org/10.1016/j.indcrop.2018.03.019

Anicić N, Matekalo D, Skorić M, Pećinar I, Brkusanin M, Nestorović-Zivković J, Dmitrović S, Dajić-Stevanović Z, Schulz H, Misić D. Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products. 2018;117:347-358.
doi:10.1016/j.indcrop.2018.03.019 .

Anicić, Neda, Matekalo, Dragana, Skorić, Marijana, Pećinar, Ilinka, Brkusanin, Miloš, Nestorović-Zivković, Jasmina, Dmitrović, Slavica, Dajić-Stevanović, Zora, Schulz, Hartwig, Misić, Danijela, "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants" in Industrial Crops and Products, 117 (2018):347-358,
https://doi.org/10.1016/j.indcrop.2018.03.019 . .

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Šoštarić, Ivan
AU  - Razić, Slavica
AU  - Milenković, Marina
AU  - Couladis, Maria
AU  - Maksimović, Zoran
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4027
AB  - Hungarian thyme (Thymus pannonicus All., Lamiaceae) is an aromatic herb used as traditional remedy, a refreshing beverage and a food aromatizer. Herbal teas, i.e., infusions, of Hungarian thyme from eight localities in Serbia were analyzed regarding their polyphenolic and volatile composition, and tested for their antioxidant and antimicrobial activity. The total polyphenolics content, determined by the Folin-Ciocalteu method, ranged from 1122.25 to 1979.93 mg gallic acid/L. HPLC analysis revealed rosmarinic acid (367.42-1199.47 mg/L) and luteolin glucuronides as the main polyphenolics. The volatile fractions of the infusions, analyzed by static headspace extraction coupled with GC and GC-MS analyses, contained citral, 3-octanone, 1-octen-3-ol, linalool and 1,8-cineole as the dominant constituents. The antioxidant activity of the infusions was examined through the ferric reducing antioxidant power (FRAP) (68.09-124.58 mmol Fe2+/L) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical tests (SC50 1.32-2.96 mu L/mL). The antimicrobial activity was tested by the broth microdilution method against standard strains of Gram(+) Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Enterococcus faecalis and Gram(-) bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, and one strain of yeast Candida albicans. The infusions inhibited microbial growth in the tested concentration range (31.25-500.00 mu L/mL) and the strongest activity was exhibited against the strain of C. albicans (MIC 31.25-62.50 mu L/mL). The antioxidant and antimicrobial properties of the infusions were, to some extent, in correlation with the composition and content of the polyphenolic compounds, whereas the volatiles noticeably influenced the exhibited antimicrobial activity.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics
EP  - 20
SP  - 14
VL  - 89
DO  - 10.1016/j.indcrop.2016.04.046
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Šoštarić, Ivan and Razić, Slavica and Milenković, Marina and Couladis, Maria and Maksimović, Zoran",
year = "2016",
abstract = "Hungarian thyme (Thymus pannonicus All., Lamiaceae) is an aromatic herb used as traditional remedy, a refreshing beverage and a food aromatizer. Herbal teas, i.e., infusions, of Hungarian thyme from eight localities in Serbia were analyzed regarding their polyphenolic and volatile composition, and tested for their antioxidant and antimicrobial activity. The total polyphenolics content, determined by the Folin-Ciocalteu method, ranged from 1122.25 to 1979.93 mg gallic acid/L. HPLC analysis revealed rosmarinic acid (367.42-1199.47 mg/L) and luteolin glucuronides as the main polyphenolics. The volatile fractions of the infusions, analyzed by static headspace extraction coupled with GC and GC-MS analyses, contained citral, 3-octanone, 1-octen-3-ol, linalool and 1,8-cineole as the dominant constituents. The antioxidant activity of the infusions was examined through the ferric reducing antioxidant power (FRAP) (68.09-124.58 mmol Fe2+/L) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical tests (SC50 1.32-2.96 mu L/mL). The antimicrobial activity was tested by the broth microdilution method against standard strains of Gram(+) Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Enterococcus faecalis and Gram(-) bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, and one strain of yeast Candida albicans. The infusions inhibited microbial growth in the tested concentration range (31.25-500.00 mu L/mL) and the strongest activity was exhibited against the strain of C. albicans (MIC 31.25-62.50 mu L/mL). The antioxidant and antimicrobial properties of the infusions were, to some extent, in correlation with the composition and content of the polyphenolic compounds, whereas the volatiles noticeably influenced the exhibited antimicrobial activity.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics",
pages = "20-14",
volume = "89",
doi = "10.1016/j.indcrop.2016.04.046"
}

Arsenijević, J., Drobac, M., Šoštarić, I., Razić, S., Milenković, M., Couladis, M.,& Maksimović, Z.. (2016). Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics. in Industrial Crops and Products
Elsevier, Amsterdam., 89, 14-20.
https://doi.org/10.1016/j.indcrop.2016.04.046

Arsenijević J, Drobac M, Šoštarić I, Razić S, Milenković M, Couladis M, Maksimović Z. Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics. in Industrial Crops and Products. 2016;89:14-20.
doi:10.1016/j.indcrop.2016.04.046 .

Arsenijević, Jelena, Drobac, Milica, Šoštarić, Ivan, Razić, Slavica, Milenković, Marina, Couladis, Maria, Maksimović, Zoran, "Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics" in Industrial Crops and Products, 89 (2016):14-20,
https://doi.org/10.1016/j.indcrop.2016.04.046 . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Pljevljakusić, Dejan
AU  - Ristić, Mihajlo
AU  - Šoštarić, Ivan
AU  - Kresović, Mirjana
AU  - Simić, Ivana
AU  - Vrbničanin, Sava
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3748
AB  - Essential oil yield and composition of three populations of yarrow (Achillea millefolium aggregate) occurring on saline habitats of Serbia was analyzed in relation to its soil site characteristics. The total of 99 compounds of essential oils was determined within three estimated chemotypes: the chamazulene + trans-caryophyllene+-pinene (in population assigned as P1), the lavandulyl acetate+chamazulene + trans-caryophyllene (in P2) and the trans-chrysanthenyl acetate+trans-caryophyllene+germacrene D (in P3). The highest yield of 1.01 % and the lowest of 0.32 % were determined for populations P1 and P3, respectively. The highest total salt content, corresponding with the highest values of ECe and sodium, chloride and sulfate ion concentration was determined for soil of P1 population, which was assessed as solonchak soil, in difference to much lower value of sodium ions, total salts, ECe and pH, all increasing with the soil depth, as determined for samples of P2 and P3 yarrow populations - typical for the solonetz type of salt affected soils. Differences in yield and chemical composition of essential oils were discussed in term of impact of both population's genetic variability and specific environmental conditions. The essential oil quality in all researched yarrow populations accomplishes standards of EU Pharmacopeia 6.0 and thus, sustainable wild collection of yarrow from halophytic habitats could be recommended.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia
EP  - 1352
IS  - 6
SP  - 1343
VL  - 18
DO  - 10.1080/0972060X.2014.884952
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Pljevljakusić, Dejan and Ristić, Mihajlo and Šoštarić, Ivan and Kresović, Mirjana and Simić, Ivana and Vrbničanin, Sava",
year = "2015",
abstract = "Essential oil yield and composition of three populations of yarrow (Achillea millefolium aggregate) occurring on saline habitats of Serbia was analyzed in relation to its soil site characteristics. The total of 99 compounds of essential oils was determined within three estimated chemotypes: the chamazulene + trans-caryophyllene+-pinene (in population assigned as P1), the lavandulyl acetate+chamazulene + trans-caryophyllene (in P2) and the trans-chrysanthenyl acetate+trans-caryophyllene+germacrene D (in P3). The highest yield of 1.01 % and the lowest of 0.32 % were determined for populations P1 and P3, respectively. The highest total salt content, corresponding with the highest values of ECe and sodium, chloride and sulfate ion concentration was determined for soil of P1 population, which was assessed as solonchak soil, in difference to much lower value of sodium ions, total salts, ECe and pH, all increasing with the soil depth, as determined for samples of P2 and P3 yarrow populations - typical for the solonetz type of salt affected soils. Differences in yield and chemical composition of essential oils were discussed in term of impact of both population's genetic variability and specific environmental conditions. The essential oil quality in all researched yarrow populations accomplishes standards of EU Pharmacopeia 6.0 and thus, sustainable wild collection of yarrow from halophytic habitats could be recommended.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia",
pages = "1352-1343",
number = "6",
volume = "18",
doi = "10.1080/0972060X.2014.884952"
}

Dajić-Stevanović, Z., Pljevljakusić, D., Ristić, M., Šoštarić, I., Kresović, M., Simić, I.,& Vrbničanin, S.. (2015). Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 18(6), 1343-1352.
https://doi.org/10.1080/0972060X.2014.884952

Dajić-Stevanović Z, Pljevljakusić D, Ristić M, Šoštarić I, Kresović M, Simić I, Vrbničanin S. Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia. in Journal of Essential Oil Bearing Plants. 2015;18(6):1343-1352.
doi:10.1080/0972060X.2014.884952 .

Dajić-Stevanović, Zora, Pljevljakusić, Dejan, Ristić, Mihajlo, Šoštarić, Ivan, Kresović, Mirjana, Simić, Ivana, Vrbničanin, Sava, "Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia" in Journal of Essential Oil Bearing Plants, 18, no. 6 (2015):1343-1352,
https://doi.org/10.1080/0972060X.2014.884952 . .

TY  - JOUR
AU  - Stanković, Milan S.
AU  - Petrović, Milica
AU  - Godjevac, Dejan
AU  - Dajić-Stevanović, Zora
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3837
AB  - This paper presents new data for secondary metabolites and biological activity of the insufficiently studied European inland halophytes as remarkable indicators of Balkan saline habitats. Phenolic content, flavonoid concentrations and antioxidant activity were analyzed in fifteen terrestrial salt tolerant plants collected from eight saline habitats situated in two distinct semiarid regions of Serbia. Total phenols ranged from 31.86 to 212.71 mg of GA/g of extract (gallic acid equivalent milligrams of gallic acid per gram of extract). Concentration of flavonoids ranged from 41.21 to 146.06 mg of RU/g of extract (rutin equivalent milligrams of rutin per gram of extract). Antioxidant activity was determined using 1,1-dipheny1-2-picrylhydrazyl radical reagent; obtained values was ranged from 1785.81 to 17.55 mu g/ml. A significant relation was noticed between the concentration of phenols and antioxidant activity. Parallel to the analysis of researched halophytes, Camellia sinensis and Ginkgo biloba were analyzed for comparison. The best antioxidant properties were determined for Statice gmelinii, Artemisia santonicum and Mentha pulegium. The results indicate a potential of studied halophytic species as candidates for natural sources of secondary metabolites with high biological activity, whereas their bioactive compounds might have a significant role in adaptations to salt and drought stress.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Arid Environments
T1  - Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants?
EP  - 32
SP  - 26
VL  - 120
DO  - 10.1016/j.jaridenv.2015.04.008
ER  - 

@article{
author = "Stanković, Milan S. and Petrović, Milica and Godjevac, Dejan and Dajić-Stevanović, Zora",
year = "2015",
abstract = "This paper presents new data for secondary metabolites and biological activity of the insufficiently studied European inland halophytes as remarkable indicators of Balkan saline habitats. Phenolic content, flavonoid concentrations and antioxidant activity were analyzed in fifteen terrestrial salt tolerant plants collected from eight saline habitats situated in two distinct semiarid regions of Serbia. Total phenols ranged from 31.86 to 212.71 mg of GA/g of extract (gallic acid equivalent milligrams of gallic acid per gram of extract). Concentration of flavonoids ranged from 41.21 to 146.06 mg of RU/g of extract (rutin equivalent milligrams of rutin per gram of extract). Antioxidant activity was determined using 1,1-dipheny1-2-picrylhydrazyl radical reagent; obtained values was ranged from 1785.81 to 17.55 mu g/ml. A significant relation was noticed between the concentration of phenols and antioxidant activity. Parallel to the analysis of researched halophytes, Camellia sinensis and Ginkgo biloba were analyzed for comparison. The best antioxidant properties were determined for Statice gmelinii, Artemisia santonicum and Mentha pulegium. The results indicate a potential of studied halophytic species as candidates for natural sources of secondary metabolites with high biological activity, whereas their bioactive compounds might have a significant role in adaptations to salt and drought stress.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Arid Environments",
title = "Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants?",
pages = "32-26",
volume = "120",
doi = "10.1016/j.jaridenv.2015.04.008"
}

Stanković, M. S., Petrović, M., Godjevac, D.,& Dajić-Stevanović, Z.. (2015). Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants?. in Journal of Arid Environments
Academic Press Ltd- Elsevier Science Ltd, London., 120, 26-32.
https://doi.org/10.1016/j.jaridenv.2015.04.008

Stanković MS, Petrović M, Godjevac D, Dajić-Stevanović Z. Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants?. in Journal of Arid Environments. 2015;120:26-32.
doi:10.1016/j.jaridenv.2015.04.008 .

Stanković, Milan S., Petrović, Milica, Godjevac, Dejan, Dajić-Stevanović, Zora, "Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants?" in Journal of Arid Environments, 120 (2015):26-32,
https://doi.org/10.1016/j.jaridenv.2015.04.008 . .

TY  - CHAP
AU  - Stevanović, Z.D.
AU  - Petrović, M.
AU  - Aćić, Svetlana
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3489
AB  - In Serbian folk tradition, hundreds of plants were used for ages as food, beverages, medicine, natural dyes, natural additives, and food preservatives, for textile and fibers, shelter and fuel, as well as for traditional customs, religious purposes, and magic. Despite being often treated as a problem for the national economy, rural areas in Serbia actually represent a huge potential, namely in richness of natural resources, i.e., (agro)biodiversity, as well as cultural and traditional heritage and related ethnobotanical knowledge. The history of health culture of the Balkan nations is very complex and interesting. The extremely long tradition of curative and prophylactic use of medicinal herbs is mentioned in the folk medical texts of both known and unknown authors. The Old Serbs had a very developed cult dedicated to certain trees and plants, where many of customs remain in place even today. Here, we discuss the most interesting, old, and autochthonous varieties of cereals, vegetables, and fruits that are still used in traditional Serbian cuisine today. This is followed with a brief review of the most commonly used medicinal herbs in Serbian folk medicine, as well as an outline of several of the best-described magical plants, including oak, basil, nettle, wormwood, linden, hawthorn, hazel tree, and laserwort.
PB  - Springer New York
T2  - Ethnobotany and Biocultural Diversities in the Balkans: Perspectives on Sustainable Rural Developmen
T1  - Ethnobotanical knowledge and traditional use of plants in Serbia in relation to sustainable rural development
EP  - 252
SP  - 229
DO  - 10.1007/978-1-4939-1492-0_12
ER  - 

@inbook{
author = "Stevanović, Z.D. and Petrović, M. and Aćić, Svetlana",
year = "2014",
abstract = "In Serbian folk tradition, hundreds of plants were used for ages as food, beverages, medicine, natural dyes, natural additives, and food preservatives, for textile and fibers, shelter and fuel, as well as for traditional customs, religious purposes, and magic. Despite being often treated as a problem for the national economy, rural areas in Serbia actually represent a huge potential, namely in richness of natural resources, i.e., (agro)biodiversity, as well as cultural and traditional heritage and related ethnobotanical knowledge. The history of health culture of the Balkan nations is very complex and interesting. The extremely long tradition of curative and prophylactic use of medicinal herbs is mentioned in the folk medical texts of both known and unknown authors. The Old Serbs had a very developed cult dedicated to certain trees and plants, where many of customs remain in place even today. Here, we discuss the most interesting, old, and autochthonous varieties of cereals, vegetables, and fruits that are still used in traditional Serbian cuisine today. This is followed with a brief review of the most commonly used medicinal herbs in Serbian folk medicine, as well as an outline of several of the best-described magical plants, including oak, basil, nettle, wormwood, linden, hawthorn, hazel tree, and laserwort.",
publisher = "Springer New York",
journal = "Ethnobotany and Biocultural Diversities in the Balkans: Perspectives on Sustainable Rural Developmen",
booktitle = "Ethnobotanical knowledge and traditional use of plants in Serbia in relation to sustainable rural development",
pages = "252-229",
doi = "10.1007/978-1-4939-1492-0_12"
}

Stevanović, Z.D., Petrović, M.,& Aćić, S.. (2014). Ethnobotanical knowledge and traditional use of plants in Serbia in relation to sustainable rural development. in Ethnobotany and Biocultural Diversities in the Balkans: Perspectives on Sustainable Rural Developmen
Springer New York., 229-252.
https://doi.org/10.1007/978-1-4939-1492-0_12

Stevanović Z, Petrović M, Aćić S. Ethnobotanical knowledge and traditional use of plants in Serbia in relation to sustainable rural development. in Ethnobotany and Biocultural Diversities in the Balkans: Perspectives on Sustainable Rural Developmen. 2014;:229-252.
doi:10.1007/978-1-4939-1492-0_12 .

Stevanović, Z.D., Petrović, M., Aćić, Svetlana, "Ethnobotanical knowledge and traditional use of plants in Serbia in relation to sustainable rural development" in Ethnobotany and Biocultural Diversities in the Balkans: Perspectives on Sustainable Rural Developmen (2014):229-252,
https://doi.org/10.1007/978-1-4939-1492-0_12 . .

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Marković, Jelena
AU  - Šoštarić, Ivan
AU  - Razić, Slavica
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3141
AB  - The volatile fraction of the leaves of Thymus pannonicus All. (Lamiaceae) was analyzed by headspace extraction followed by GC-FID and GC-MS analysis. The different headspace profiles were recognized, with citral and with monoterpene hydrocarbons as dominant compounds. In addition, the determination of Cr, Co, Ni, Mo, Cu, Zn, Mn, Fe, Mg, Ca, K and Na was conducted by spectroscopic techniques (FAAS, GFAAS and ICP-OES). In order to evaluate the relationship between volatile organic compounds and metals, a chemometrics approach was applied. The data obtained by analysis of the headspace and elemental content were subjected to correlation analysis, factor analysis, principal component analysis and cluster analysis. A number of significant correlations of metals with plant volatiles were found. Correlation of Zn with citral, Mn with oxygenated monoterpenes and Mg with beta-bourbonene, could be explained by involvement of metals in the biosynthesis of volatile organic compounds.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - Plant Physiology and Biochemistry
T1  - A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples
EP  - 306
SP  - 298
VL  - 71
DO  - 10.1016/j.plaphy.2013.08.002
ER  - 

@article{
author = "Arsenijević, Jelena and Marković, Jelena and Šoštarić, Ivan and Razić, Slavica",
year = "2013",
abstract = "The volatile fraction of the leaves of Thymus pannonicus All. (Lamiaceae) was analyzed by headspace extraction followed by GC-FID and GC-MS analysis. The different headspace profiles were recognized, with citral and with monoterpene hydrocarbons as dominant compounds. In addition, the determination of Cr, Co, Ni, Mo, Cu, Zn, Mn, Fe, Mg, Ca, K and Na was conducted by spectroscopic techniques (FAAS, GFAAS and ICP-OES). In order to evaluate the relationship between volatile organic compounds and metals, a chemometrics approach was applied. The data obtained by analysis of the headspace and elemental content were subjected to correlation analysis, factor analysis, principal component analysis and cluster analysis. A number of significant correlations of metals with plant volatiles were found. Correlation of Zn with citral, Mn with oxygenated monoterpenes and Mg with beta-bourbonene, could be explained by involvement of metals in the biosynthesis of volatile organic compounds.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "Plant Physiology and Biochemistry",
title = "A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples",
pages = "306-298",
volume = "71",
doi = "10.1016/j.plaphy.2013.08.002"
}

Arsenijević, J., Marković, J., Šoštarić, I.,& Razić, S.. (2013). A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 71, 298-306.
https://doi.org/10.1016/j.plaphy.2013.08.002

Arsenijević J, Marković J, Šoštarić I, Razić S. A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples. in Plant Physiology and Biochemistry. 2013;71:298-306.
doi:10.1016/j.plaphy.2013.08.002 .

Arsenijević, Jelena, Marković, Jelena, Šoštarić, Ivan, Razić, Slavica, "A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples" in Plant Physiology and Biochemistry, 71 (2013):298-306,
https://doi.org/10.1016/j.plaphy.2013.08.002 . .

TY  - THES
AU  - Šoštarić, Ivan
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/9
AB  - In terms of the number of species, the genus Thymus is one of the most important genera within the Lamiaceae family. The number of species may vary depending on the approach of the author, but if the concept of minimal variability is chosen 215 species have been described. This genus is divided into 8 sections. In Flora of Serbia, 31 species and more than 100 ifraspecific taxa have been described, all of which belong to section Serpyllum, except Thymus comptus and Th. striatus (Sect. Hyphodromi) and Th. vulgaris (Sect. Thymus). Reproductive isolation between species seems to be weak, which makes taxonomical studies in this genus even more difficult. Species of genus Thymus are used as aromatic and medicinal plants in both traditional and modern medicine in all regions where they grow. The aim of this work was to describe different chemotypes within species and population of this genus and, by analysis of surface flavonoids and AFLP markers, provide better insight into genetic and evolutional relationships among the populations and species of the genus Thymus from Serbia. This work included populations of Th. pulegioides (Subsect. Alternantes), Th. glabrescens, Th. maschallianus and Th. pannonicus (Subsect. Isolepides), Th. balcanus and Th. praecox (Subsect. Peudomarginati). Analysis of essential oils was done by TD-GC-MS system, and compounds were identified by comparing RI and/or mass spectra with published data. 103 compounds were identified. Three major group of essential oil compounds were identified: monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Based on relative abundance of compounds, Corelation, PCA and Cluster analysis were performed, and it revealed the existence of several chemotypes, such as geraniol, phenol, germacrene-D, citral, linalool, (E)-caryophyllene, α-terpinyl acetate and linalyl acetate chemotype.
AB  - Rod Thymus je po broju vrsta jedan od najznaĉajnijih u okviru familije Lamiaceae. U zavisnosti od pristupa autora broj vrsta unutar roda varira, meĊutim ukoliko se izabere pristup minimalne varijabilnosti, prema raspoloţivim podacima opisano je 215 vrsta ovog roda. Vrste ovog roda podeljene su u osam sekcija. U flori Srbije opisana je, do sada, 31 vrsta i preko 100 infraspecijskih taksona, koje sve pripadaju podsekciji Serpyllum, sa izuzetkom vrsta Thymus comptus i Th. striatus (Sect. Hyphodromi) i Th. vulgaris (Sect. Thymus). Reproduktivna izolacija izmeĊu vrsta ovog roda je izuzetno slaba što ĉini taksonomska istraţivanja još teţim. Vrste roda Thymus koriste se kao lekovite i aromatiĉne biljke u tradicionalnoj i zvaniĉnoj medicini u svim krajevima u kojima se javljaju. Cilj ovog rada je bio da se opišu razliĉiti hemotipovi unutar vrsta i populaciju ovog roda kao i da se analizom površinskih flavonoida i AFLP markera poboljša uvid u genetiĉke i evolutivne odnose izmeĊu populacija vrsta ovog roda sa podruĉja Srbije. U ovo istrţivajne ukljuĉene su populacije vrsta Th. pulegioides (Subsect. Alternantes), Th. glabrescens, Th. maschallianus i Th. pannonicus (Subsect. Isolepides), Th. balcanus i Th. praecox (Subsect. Peudomarginati) Analiza sastava etarskih ulja vršena je pomoću TD-GC-MS sistema, a jedinjenja su odreĊen uporeĊivanjem RI i/ili masenog spektra sa literaturnim podacima. Identifikovana su ukupno 103 jedinjenja. Identifikovane su, po brojnosti, tri glavne grupe jedinjenja: seskviterpenski ugljovodonici, oksigenovani monoterpenski ugljovodonici i monoterpenski ugljovodonici. Statistiĉka obrada sastava etarskih ulja ukljuĉila je korelacije, analizu glavnih komponenti i klaster analizu. Statistiĉka obrada je pokazala da se, po relativnoj zastupljenosti jedinjenja, mogu razlikovati geraniolni, fenolni, germakren-D, citralni, linaloolni, (E)-kariofilen, α-terpinil acetat i linalil acetatni hemotip.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Phytochemical and genetical variability of species from section Serpyllum (Mill.) Benth. genus Thymus L. (Lamiaceae) in Serbia
T1  - Fitohemijska i genetička varijabilnost vrsta iz sekcije Serpyllum (Mill.) Benth. roda Thymus L. (Lamiaceae) u Srbiji
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2025
ER  - 

@phdthesis{
author = "Šoštarić, Ivan",
year = "2012",
abstract = "In terms of the number of species, the genus Thymus is one of the most important genera within the Lamiaceae family. The number of species may vary depending on the approach of the author, but if the concept of minimal variability is chosen 215 species have been described. This genus is divided into 8 sections. In Flora of Serbia, 31 species and more than 100 ifraspecific taxa have been described, all of which belong to section Serpyllum, except Thymus comptus and Th. striatus (Sect. Hyphodromi) and Th. vulgaris (Sect. Thymus). Reproductive isolation between species seems to be weak, which makes taxonomical studies in this genus even more difficult. Species of genus Thymus are used as aromatic and medicinal plants in both traditional and modern medicine in all regions where they grow. The aim of this work was to describe different chemotypes within species and population of this genus and, by analysis of surface flavonoids and AFLP markers, provide better insight into genetic and evolutional relationships among the populations and species of the genus Thymus from Serbia. This work included populations of Th. pulegioides (Subsect. Alternantes), Th. glabrescens, Th. maschallianus and Th. pannonicus (Subsect. Isolepides), Th. balcanus and Th. praecox (Subsect. Peudomarginati). Analysis of essential oils was done by TD-GC-MS system, and compounds were identified by comparing RI and/or mass spectra with published data. 103 compounds were identified. Three major group of essential oil compounds were identified: monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Based on relative abundance of compounds, Corelation, PCA and Cluster analysis were performed, and it revealed the existence of several chemotypes, such as geraniol, phenol, germacrene-D, citral, linalool, (E)-caryophyllene, α-terpinyl acetate and linalyl acetate chemotype., Rod Thymus je po broju vrsta jedan od najznaĉajnijih u okviru familije Lamiaceae. U zavisnosti od pristupa autora broj vrsta unutar roda varira, meĊutim ukoliko se izabere pristup minimalne varijabilnosti, prema raspoloţivim podacima opisano je 215 vrsta ovog roda. Vrste ovog roda podeljene su u osam sekcija. U flori Srbije opisana je, do sada, 31 vrsta i preko 100 infraspecijskih taksona, koje sve pripadaju podsekciji Serpyllum, sa izuzetkom vrsta Thymus comptus i Th. striatus (Sect. Hyphodromi) i Th. vulgaris (Sect. Thymus). Reproduktivna izolacija izmeĊu vrsta ovog roda je izuzetno slaba što ĉini taksonomska istraţivanja još teţim. Vrste roda Thymus koriste se kao lekovite i aromatiĉne biljke u tradicionalnoj i zvaniĉnoj medicini u svim krajevima u kojima se javljaju. Cilj ovog rada je bio da se opišu razliĉiti hemotipovi unutar vrsta i populaciju ovog roda kao i da se analizom površinskih flavonoida i AFLP markera poboljša uvid u genetiĉke i evolutivne odnose izmeĊu populacija vrsta ovog roda sa podruĉja Srbije. U ovo istrţivajne ukljuĉene su populacije vrsta Th. pulegioides (Subsect. Alternantes), Th. glabrescens, Th. maschallianus i Th. pannonicus (Subsect. Isolepides), Th. balcanus i Th. praecox (Subsect. Peudomarginati) Analiza sastava etarskih ulja vršena je pomoću TD-GC-MS sistema, a jedinjenja su odreĊen uporeĊivanjem RI i/ili masenog spektra sa literaturnim podacima. Identifikovana su ukupno 103 jedinjenja. Identifikovane su, po brojnosti, tri glavne grupe jedinjenja: seskviterpenski ugljovodonici, oksigenovani monoterpenski ugljovodonici i monoterpenski ugljovodonici. Statistiĉka obrada sastava etarskih ulja ukljuĉila je korelacije, analizu glavnih komponenti i klaster analizu. Statistiĉka obrada je pokazala da se, po relativnoj zastupljenosti jedinjenja, mogu razlikovati geraniolni, fenolni, germakren-D, citralni, linaloolni, (E)-kariofilen, α-terpinil acetat i linalil acetatni hemotip.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Phytochemical and genetical variability of species from section Serpyllum (Mill.) Benth. genus Thymus L. (Lamiaceae) in Serbia, Fitohemijska i genetička varijabilnost vrsta iz sekcije Serpyllum (Mill.) Benth. roda Thymus L. (Lamiaceae) u Srbiji",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2025"
}

Šoštarić, I.. (2012). Phytochemical and genetical variability of species from section Serpyllum (Mill.) Benth. genus Thymus L. (Lamiaceae) in Serbia. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_2025

Šoštarić I. Phytochemical and genetical variability of species from section Serpyllum (Mill.) Benth. genus Thymus L. (Lamiaceae) in Serbia. 2012;.
https://hdl.handle.net/21.15107/rcub_nardus_2025 .

Šoštarić, Ivan, "Phytochemical and genetical variability of species from section Serpyllum (Mill.) Benth. genus Thymus L. (Lamiaceae) in Serbia" (2012),
https://hdl.handle.net/21.15107/rcub_nardus_2025 .

TY  - JOUR
AU  - Šoštarić, Ivan
AU  - Arsenijević, Jelena
AU  - Aćić, Svetlana
AU  - Dajić-Stevanović, Zora
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2767
AB  - Three populations of Thymus pannonicus grown in Serbia (one from northern and two from east Serbia) were examined for chemical composition of their essential oil. Thymus pannonicus is used in traditional medicine and it is collected by local people in the studied regions, hence the interest in composition of its essential oils. Three major groups of essential oil components were identified, such as monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Each of investigated populations showed its own chemotype, including: geranial (citral), alpha-pinene and germacrene-D. To best of our knowledge, this sesquiterpene hydrocarbon was reported as a major component in Thymus bracteosus only. In these populations the phenolic compounds thymol and carvacrol, considered as most frequent constituents of essential oil in Thymus taxa, haven't been found in significant quantities.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia
EP  - 243
IS  - 2
SP  - 237
VL  - 15
DO  - 10.1080/0972060X.2012.10644041
ER  - 

@article{
author = "Šoštarić, Ivan and Arsenijević, Jelena and Aćić, Svetlana and Dajić-Stevanović, Zora",
year = "2012",
abstract = "Three populations of Thymus pannonicus grown in Serbia (one from northern and two from east Serbia) were examined for chemical composition of their essential oil. Thymus pannonicus is used in traditional medicine and it is collected by local people in the studied regions, hence the interest in composition of its essential oils. Three major groups of essential oil components were identified, such as monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Each of investigated populations showed its own chemotype, including: geranial (citral), alpha-pinene and germacrene-D. To best of our knowledge, this sesquiterpene hydrocarbon was reported as a major component in Thymus bracteosus only. In these populations the phenolic compounds thymol and carvacrol, considered as most frequent constituents of essential oil in Thymus taxa, haven't been found in significant quantities.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia",
pages = "243-237",
number = "2",
volume = "15",
doi = "10.1080/0972060X.2012.10644041"
}

Šoštarić, I., Arsenijević, J., Aćić, S.,& Dajić-Stevanović, Z.. (2012). Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 15(2), 237-243.
https://doi.org/10.1080/0972060X.2012.10644041

Šoštarić I, Arsenijević J, Aćić S, Dajić-Stevanović Z. Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia. in Journal of Essential Oil Bearing Plants. 2012;15(2):237-243.
doi:10.1080/0972060X.2012.10644041 .

Šoštarić, Ivan, Arsenijević, Jelena, Aćić, Svetlana, Dajić-Stevanović, Zora, "Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia" in Journal of Essential Oil Bearing Plants, 15, no. 2 (2012):237-243,
https://doi.org/10.1080/0972060X.2012.10644041 . .

TY  - JOUR
AU  - Šoštarić, Ivan
AU  - Liber, Zlatko
AU  - Grdisa, Martina
AU  - Marin, Petar D.
AU  - Dajić-Stevanović, Zora
AU  - Satović, Zlatko
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3005
AB  - Amplified fragment length polymorphism (AFLP) markers were used to assess genetic relations of 32 Thymus populations belonging to seven species of genus Thymus, section Serpyllum, from Serbia. The collected species belong to three subsections: (1) Alternantes Klokov (Th. pulegioides L.), (2) Isolepides (Borbas) Halacsy (Th. glabrescens Wind.. Th. marschallianus Willd., Th. pannonicus All.), and (3) Pseudo-marginati (Braun ex Borbas) Jalas (Th. balcanus Borbas, Th. moesiacus Velen., Th. praecox Opiz). Analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, while the remaining variability was almost equally distributed among species and among populations within species. The pairwise AMOVA analyses further showed that species differentiation was significant in all cases, except between Th. marschallianus and Th. pannonicus. All the individuals belonging to Th. pulegioides (subsection Alternantes) formed a well-supported clade, the most divergent from all other species in this study. This genetic differentiation of Th. pulegioides is supported by its morphological features because it is the only analyzed taxon having hairs only on the stem edges, and can easily be recognized by this character. According to our results, the subsection Isolepides is polyphyletic. Th. glabrescens is clearly separated from the other two species of this subsection (Th. marschallianus, Th. pannonicus). Although morphologically distinguishable by leaf indumentum, individuals belonging to Th. marschallianus and Th. pannonicus could not be differentiated at the molecular level. The species belonging to subsection Pseudomarginati formed a monophyletic clade, although not supported by high bootstrap value. The clade is further divided into three well-supported clades representing distinct species (Th. balcanus, Th. praecox and Th. moesiacus). In our study, the AFLP markers demonstrated that they could be suitable for studying complex genetic relationships, including frequent interspecies hybridization events, although strict reticulate evolutionary history could not be determined. All the population genetic parameters in the section Serpyllum suggest that the reproductive incompatibility between the species is very weak, which indicates a strong reticulating system, especially in the case when two or more species occur sympatrically.
PB  - Elsevier Gmbh, Munich
T2  - Flora
T1  - Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)
EP  - 661
IS  - 9
SP  - 654
VL  - 207
DO  - 10.1016/j.flora.2012.06.018
ER  - 

@article{
author = "Šoštarić, Ivan and Liber, Zlatko and Grdisa, Martina and Marin, Petar D. and Dajić-Stevanović, Zora and Satović, Zlatko",
year = "2012",
abstract = "Amplified fragment length polymorphism (AFLP) markers were used to assess genetic relations of 32 Thymus populations belonging to seven species of genus Thymus, section Serpyllum, from Serbia. The collected species belong to three subsections: (1) Alternantes Klokov (Th. pulegioides L.), (2) Isolepides (Borbas) Halacsy (Th. glabrescens Wind.. Th. marschallianus Willd., Th. pannonicus All.), and (3) Pseudo-marginati (Braun ex Borbas) Jalas (Th. balcanus Borbas, Th. moesiacus Velen., Th. praecox Opiz). Analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, while the remaining variability was almost equally distributed among species and among populations within species. The pairwise AMOVA analyses further showed that species differentiation was significant in all cases, except between Th. marschallianus and Th. pannonicus. All the individuals belonging to Th. pulegioides (subsection Alternantes) formed a well-supported clade, the most divergent from all other species in this study. This genetic differentiation of Th. pulegioides is supported by its morphological features because it is the only analyzed taxon having hairs only on the stem edges, and can easily be recognized by this character. According to our results, the subsection Isolepides is polyphyletic. Th. glabrescens is clearly separated from the other two species of this subsection (Th. marschallianus, Th. pannonicus). Although morphologically distinguishable by leaf indumentum, individuals belonging to Th. marschallianus and Th. pannonicus could not be differentiated at the molecular level. The species belonging to subsection Pseudomarginati formed a monophyletic clade, although not supported by high bootstrap value. The clade is further divided into three well-supported clades representing distinct species (Th. balcanus, Th. praecox and Th. moesiacus). In our study, the AFLP markers demonstrated that they could be suitable for studying complex genetic relationships, including frequent interspecies hybridization events, although strict reticulate evolutionary history could not be determined. All the population genetic parameters in the section Serpyllum suggest that the reproductive incompatibility between the species is very weak, which indicates a strong reticulating system, especially in the case when two or more species occur sympatrically.",
publisher = "Elsevier Gmbh, Munich",
journal = "Flora",
title = "Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)",
pages = "661-654",
number = "9",
volume = "207",
doi = "10.1016/j.flora.2012.06.018"
}

Šoštarić, I., Liber, Z., Grdisa, M., Marin, P. D., Dajić-Stevanović, Z.,& Satović, Z.. (2012). Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum). in Flora
Elsevier Gmbh, Munich., 207(9), 654-661.
https://doi.org/10.1016/j.flora.2012.06.018

Šoštarić I, Liber Z, Grdisa M, Marin PD, Dajić-Stevanović Z, Satović Z. Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum). in Flora. 2012;207(9):654-661.
doi:10.1016/j.flora.2012.06.018 .

Šoštarić, Ivan, Liber, Zlatko, Grdisa, Martina, Marin, Petar D., Dajić-Stevanović, Zora, Satović, Zlatko, "Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)" in Flora, 207, no. 9 (2012):654-661,
https://doi.org/10.1016/j.flora.2012.06.018 . .