TY  - JOUR
AU  - Resetnik, Ivana
AU  - Baricević, Dea
AU  - Rusu, Diana Batir
AU  - Carović-Stanko, Klaudija
AU  - Chatzopoulou, Paschalina
AU  - Dajić-Stevanović, Zora
AU  - Gonceariuc, Maria
AU  - Grdisa, Martina
AU  - Greguras, Danijela
AU  - Ibraliu, Alban
AU  - Jug-Dujaković, Marija
AU  - Krasniqi, Elez
AU  - Liber, Zlatko
AU  - Murtić, Senad
AU  - Pecanac, Dragana
AU  - Radosavljević, Ivan
AU  - Stefkov, Gjoshe
AU  - Stesević, Danijela
AU  - Šoštarić, Ivan
AU  - Satović, Zlatko
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4022
AB  - Dalmatian sage (Salvia officinalis L., Lamiaceae) is a well-known aromatic and medicinal Mediterranean plant that is native in coastal regions of the western Balkan and southern Apennine Peninsulas and is commonly cultivated worldwide. It is widely used in the food, pharmaceutical and cosmetic industries. Knowledge of its genetic diversity and spatiotemporal patterns is important for plant breeding programmes and conservation. We used eight microsatellite markers to investigate evolutionary history of indigenous populations as well as genetic diversity and structure within and among indigenous and cultivated/naturalised populations distributed across the Balkan Peninsula. The results showed a clear separation between the indigenous and cultivated/naturalised groups, with the cultivated material originating from one restricted geographical area. Most of the genetic diversity in both groups was attributable to differences among individuals within populations, although spatial genetic analysis of indigenous populations indicated the existence of isolation by distance. Geographical structuring of indigenous populations was found using clustering analysis, with three sub-clusters of indigenous populations. The highest level of gene diversity and the greatest number of private alleles were found in the central part of the eastern Adriatic coast, while decreases in gene diversity and number of private alleles were evident towards the northwestern Adriatic coast and southern and eastern regions of the Balkan Peninsula. The results of Ecological Niche Modelling during Last Glacial Maximum and Approximate Bayesian Computation suggested two plausible evolutionary trajectories: 1) the species survived in the glacial refugium in southern Adriatic coastal region with subsequent colonization events towards northern, eastern and southern Balkan Peninsula; 2) species survived in several refugia exhibiting concurrent divergence into three genetic groups. The insight into genetic diversity and structure also provide the baseline data for conservation of S. officinalis genetic resources valuable for future breeding programmes.
PB  - Public Library Science, San Francisco
T2  - Plos One
T1  - Genetic Diversity and Demographic History of Wild and Cultivated/Naturalised Plant Populations: Evidence from Dalmatian Sage (Salvia officinalis L., Lamiaceae)
IS  - 7
VL  - 11
DO  - 10.1371/journal.pone.0159545
ER  - 

@article{
author = "Resetnik, Ivana and Baricević, Dea and Rusu, Diana Batir and Carović-Stanko, Klaudija and Chatzopoulou, Paschalina and Dajić-Stevanović, Zora and Gonceariuc, Maria and Grdisa, Martina and Greguras, Danijela and Ibraliu, Alban and Jug-Dujaković, Marija and Krasniqi, Elez and Liber, Zlatko and Murtić, Senad and Pecanac, Dragana and Radosavljević, Ivan and Stefkov, Gjoshe and Stesević, Danijela and Šoštarić, Ivan and Satović, Zlatko",
year = "2016",
abstract = "Dalmatian sage (Salvia officinalis L., Lamiaceae) is a well-known aromatic and medicinal Mediterranean plant that is native in coastal regions of the western Balkan and southern Apennine Peninsulas and is commonly cultivated worldwide. It is widely used in the food, pharmaceutical and cosmetic industries. Knowledge of its genetic diversity and spatiotemporal patterns is important for plant breeding programmes and conservation. We used eight microsatellite markers to investigate evolutionary history of indigenous populations as well as genetic diversity and structure within and among indigenous and cultivated/naturalised populations distributed across the Balkan Peninsula. The results showed a clear separation between the indigenous and cultivated/naturalised groups, with the cultivated material originating from one restricted geographical area. Most of the genetic diversity in both groups was attributable to differences among individuals within populations, although spatial genetic analysis of indigenous populations indicated the existence of isolation by distance. Geographical structuring of indigenous populations was found using clustering analysis, with three sub-clusters of indigenous populations. The highest level of gene diversity and the greatest number of private alleles were found in the central part of the eastern Adriatic coast, while decreases in gene diversity and number of private alleles were evident towards the northwestern Adriatic coast and southern and eastern regions of the Balkan Peninsula. The results of Ecological Niche Modelling during Last Glacial Maximum and Approximate Bayesian Computation suggested two plausible evolutionary trajectories: 1) the species survived in the glacial refugium in southern Adriatic coastal region with subsequent colonization events towards northern, eastern and southern Balkan Peninsula; 2) species survived in several refugia exhibiting concurrent divergence into three genetic groups. The insight into genetic diversity and structure also provide the baseline data for conservation of S. officinalis genetic resources valuable for future breeding programmes.",
publisher = "Public Library Science, San Francisco",
journal = "Plos One",
title = "Genetic Diversity and Demographic History of Wild and Cultivated/Naturalised Plant Populations: Evidence from Dalmatian Sage (Salvia officinalis L., Lamiaceae)",
number = "7",
volume = "11",
doi = "10.1371/journal.pone.0159545"
}

Resetnik, I., Baricević, D., Rusu, D. B., Carović-Stanko, K., Chatzopoulou, P., Dajić-Stevanović, Z., Gonceariuc, M., Grdisa, M., Greguras, D., Ibraliu, A., Jug-Dujaković, M., Krasniqi, E., Liber, Z., Murtić, S., Pecanac, D., Radosavljević, I., Stefkov, G., Stesević, D., Šoštarić, I.,& Satović, Z.. (2016). Genetic Diversity and Demographic History of Wild and Cultivated/Naturalised Plant Populations: Evidence from Dalmatian Sage (Salvia officinalis L., Lamiaceae). in Plos One
Public Library Science, San Francisco., 11(7).
https://doi.org/10.1371/journal.pone.0159545

Resetnik I, Baricević D, Rusu DB, Carović-Stanko K, Chatzopoulou P, Dajić-Stevanović Z, Gonceariuc M, Grdisa M, Greguras D, Ibraliu A, Jug-Dujaković M, Krasniqi E, Liber Z, Murtić S, Pecanac D, Radosavljević I, Stefkov G, Stesević D, Šoštarić I, Satović Z. Genetic Diversity and Demographic History of Wild and Cultivated/Naturalised Plant Populations: Evidence from Dalmatian Sage (Salvia officinalis L., Lamiaceae). in Plos One. 2016;11(7).
doi:10.1371/journal.pone.0159545 .

Resetnik, Ivana, Baricević, Dea, Rusu, Diana Batir, Carović-Stanko, Klaudija, Chatzopoulou, Paschalina, Dajić-Stevanović, Zora, Gonceariuc, Maria, Grdisa, Martina, Greguras, Danijela, Ibraliu, Alban, Jug-Dujaković, Marija, Krasniqi, Elez, Liber, Zlatko, Murtić, Senad, Pecanac, Dragana, Radosavljević, Ivan, Stefkov, Gjoshe, Stesević, Danijela, Šoštarić, Ivan, Satović, Zlatko, "Genetic Diversity and Demographic History of Wild and Cultivated/Naturalised Plant Populations: Evidence from Dalmatian Sage (Salvia officinalis L., Lamiaceae)" in Plos One, 11, no. 7 (2016),
https://doi.org/10.1371/journal.pone.0159545 . .

TY  - JOUR
AU  - Šoštarić, Ivan
AU  - Liber, Zlatko
AU  - Grdisa, Martina
AU  - Marin, Petar D.
AU  - Dajić-Stevanović, Zora
AU  - Satović, Zlatko
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3005
AB  - Amplified fragment length polymorphism (AFLP) markers were used to assess genetic relations of 32 Thymus populations belonging to seven species of genus Thymus, section Serpyllum, from Serbia. The collected species belong to three subsections: (1) Alternantes Klokov (Th. pulegioides L.), (2) Isolepides (Borbas) Halacsy (Th. glabrescens Wind.. Th. marschallianus Willd., Th. pannonicus All.), and (3) Pseudo-marginati (Braun ex Borbas) Jalas (Th. balcanus Borbas, Th. moesiacus Velen., Th. praecox Opiz). Analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, while the remaining variability was almost equally distributed among species and among populations within species. The pairwise AMOVA analyses further showed that species differentiation was significant in all cases, except between Th. marschallianus and Th. pannonicus. All the individuals belonging to Th. pulegioides (subsection Alternantes) formed a well-supported clade, the most divergent from all other species in this study. This genetic differentiation of Th. pulegioides is supported by its morphological features because it is the only analyzed taxon having hairs only on the stem edges, and can easily be recognized by this character. According to our results, the subsection Isolepides is polyphyletic. Th. glabrescens is clearly separated from the other two species of this subsection (Th. marschallianus, Th. pannonicus). Although morphologically distinguishable by leaf indumentum, individuals belonging to Th. marschallianus and Th. pannonicus could not be differentiated at the molecular level. The species belonging to subsection Pseudomarginati formed a monophyletic clade, although not supported by high bootstrap value. The clade is further divided into three well-supported clades representing distinct species (Th. balcanus, Th. praecox and Th. moesiacus). In our study, the AFLP markers demonstrated that they could be suitable for studying complex genetic relationships, including frequent interspecies hybridization events, although strict reticulate evolutionary history could not be determined. All the population genetic parameters in the section Serpyllum suggest that the reproductive incompatibility between the species is very weak, which indicates a strong reticulating system, especially in the case when two or more species occur sympatrically.
PB  - Elsevier Gmbh, Munich
T2  - Flora
T1  - Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)
EP  - 661
IS  - 9
SP  - 654
VL  - 207
DO  - 10.1016/j.flora.2012.06.018
ER  - 

@article{
author = "Šoštarić, Ivan and Liber, Zlatko and Grdisa, Martina and Marin, Petar D. and Dajić-Stevanović, Zora and Satović, Zlatko",
year = "2012",
abstract = "Amplified fragment length polymorphism (AFLP) markers were used to assess genetic relations of 32 Thymus populations belonging to seven species of genus Thymus, section Serpyllum, from Serbia. The collected species belong to three subsections: (1) Alternantes Klokov (Th. pulegioides L.), (2) Isolepides (Borbas) Halacsy (Th. glabrescens Wind.. Th. marschallianus Willd., Th. pannonicus All.), and (3) Pseudo-marginati (Braun ex Borbas) Jalas (Th. balcanus Borbas, Th. moesiacus Velen., Th. praecox Opiz). Analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, while the remaining variability was almost equally distributed among species and among populations within species. The pairwise AMOVA analyses further showed that species differentiation was significant in all cases, except between Th. marschallianus and Th. pannonicus. All the individuals belonging to Th. pulegioides (subsection Alternantes) formed a well-supported clade, the most divergent from all other species in this study. This genetic differentiation of Th. pulegioides is supported by its morphological features because it is the only analyzed taxon having hairs only on the stem edges, and can easily be recognized by this character. According to our results, the subsection Isolepides is polyphyletic. Th. glabrescens is clearly separated from the other two species of this subsection (Th. marschallianus, Th. pannonicus). Although morphologically distinguishable by leaf indumentum, individuals belonging to Th. marschallianus and Th. pannonicus could not be differentiated at the molecular level. The species belonging to subsection Pseudomarginati formed a monophyletic clade, although not supported by high bootstrap value. The clade is further divided into three well-supported clades representing distinct species (Th. balcanus, Th. praecox and Th. moesiacus). In our study, the AFLP markers demonstrated that they could be suitable for studying complex genetic relationships, including frequent interspecies hybridization events, although strict reticulate evolutionary history could not be determined. All the population genetic parameters in the section Serpyllum suggest that the reproductive incompatibility between the species is very weak, which indicates a strong reticulating system, especially in the case when two or more species occur sympatrically.",
publisher = "Elsevier Gmbh, Munich",
journal = "Flora",
title = "Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)",
pages = "661-654",
number = "9",
volume = "207",
doi = "10.1016/j.flora.2012.06.018"
}

Šoštarić, I., Liber, Z., Grdisa, M., Marin, P. D., Dajić-Stevanović, Z.,& Satović, Z.. (2012). Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum). in Flora
Elsevier Gmbh, Munich., 207(9), 654-661.
https://doi.org/10.1016/j.flora.2012.06.018

Šoštarić I, Liber Z, Grdisa M, Marin PD, Dajić-Stevanović Z, Satović Z. Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum). in Flora. 2012;207(9):654-661.
doi:10.1016/j.flora.2012.06.018 .

Šoštarić, Ivan, Liber, Zlatko, Grdisa, Martina, Marin, Petar D., Dajić-Stevanović, Zora, Satović, Zlatko, "Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)" in Flora, 207, no. 9 (2012):654-661,
https://doi.org/10.1016/j.flora.2012.06.018 . .