Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)
Само за регистроване кориснике
2012
Аутори
Šoštarić, IvanLiber, Zlatko
Grdisa, Martina
Marin, Petar D.
Dajić-Stevanović, Zora
Satović, Zlatko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Amplified fragment length polymorphism (AFLP) markers were used to assess genetic relations of 32 Thymus populations belonging to seven species of genus Thymus, section Serpyllum, from Serbia. The collected species belong to three subsections: (1) Alternantes Klokov (Th. pulegioides L.), (2) Isolepides (Borbas) Halacsy (Th. glabrescens Wind.. Th. marschallianus Willd., Th. pannonicus All.), and (3) Pseudo-marginati (Braun ex Borbas) Jalas (Th. balcanus Borbas, Th. moesiacus Velen., Th. praecox Opiz). Analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, while the remaining variability was almost equally distributed among species and among populations within species. The pairwise AMOVA analyses further showed that species differentiation was significant in all cases, except between Th. marschallianus and Th. pannonicus. All the individuals belonging to Th. pulegioides (subsection Alternant...es) formed a well-supported clade, the most divergent from all other species in this study. This genetic differentiation of Th. pulegioides is supported by its morphological features because it is the only analyzed taxon having hairs only on the stem edges, and can easily be recognized by this character. According to our results, the subsection Isolepides is polyphyletic. Th. glabrescens is clearly separated from the other two species of this subsection (Th. marschallianus, Th. pannonicus). Although morphologically distinguishable by leaf indumentum, individuals belonging to Th. marschallianus and Th. pannonicus could not be differentiated at the molecular level. The species belonging to subsection Pseudomarginati formed a monophyletic clade, although not supported by high bootstrap value. The clade is further divided into three well-supported clades representing distinct species (Th. balcanus, Th. praecox and Th. moesiacus). In our study, the AFLP markers demonstrated that they could be suitable for studying complex genetic relationships, including frequent interspecies hybridization events, although strict reticulate evolutionary history could not be determined. All the population genetic parameters in the section Serpyllum suggest that the reproductive incompatibility between the species is very weak, which indicates a strong reticulating system, especially in the case when two or more species occur sympatrically.
Кључне речи:
Lamiaceae / AFLP / Population structure / Reticulate evolution / Balkan / MorphospeciesИзвор:
Flora, 2012, 207, 9, 654-661Издавач:
- Elsevier Gmbh, Munich
Финансирање / пројекти:
- Морфолошка, хемијска, фармаколошка и агрономска карактеризација панонског тимијана (Thymus pannonicus All., Lamiaceae), са циљем његове одрживе производње у интензивном систему ратарења (RS-MESTD-Technological Development (TD or TR)-31089)
- Scientific Research Council of the Republic of Croatia [119-1191193-1232, 178-1191193-021]
DOI: 10.1016/j.flora.2012.06.018
ISSN: 0367-2530
WoS: 000310038900004
Scopus: 2-s2.0-84866251568
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Šoštarić, Ivan AU - Liber, Zlatko AU - Grdisa, Martina AU - Marin, Petar D. AU - Dajić-Stevanović, Zora AU - Satović, Zlatko PY - 2012 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3005 AB - Amplified fragment length polymorphism (AFLP) markers were used to assess genetic relations of 32 Thymus populations belonging to seven species of genus Thymus, section Serpyllum, from Serbia. The collected species belong to three subsections: (1) Alternantes Klokov (Th. pulegioides L.), (2) Isolepides (Borbas) Halacsy (Th. glabrescens Wind.. Th. marschallianus Willd., Th. pannonicus All.), and (3) Pseudo-marginati (Braun ex Borbas) Jalas (Th. balcanus Borbas, Th. moesiacus Velen., Th. praecox Opiz). Analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, while the remaining variability was almost equally distributed among species and among populations within species. The pairwise AMOVA analyses further showed that species differentiation was significant in all cases, except between Th. marschallianus and Th. pannonicus. All the individuals belonging to Th. pulegioides (subsection Alternantes) formed a well-supported clade, the most divergent from all other species in this study. This genetic differentiation of Th. pulegioides is supported by its morphological features because it is the only analyzed taxon having hairs only on the stem edges, and can easily be recognized by this character. According to our results, the subsection Isolepides is polyphyletic. Th. glabrescens is clearly separated from the other two species of this subsection (Th. marschallianus, Th. pannonicus). Although morphologically distinguishable by leaf indumentum, individuals belonging to Th. marschallianus and Th. pannonicus could not be differentiated at the molecular level. The species belonging to subsection Pseudomarginati formed a monophyletic clade, although not supported by high bootstrap value. The clade is further divided into three well-supported clades representing distinct species (Th. balcanus, Th. praecox and Th. moesiacus). In our study, the AFLP markers demonstrated that they could be suitable for studying complex genetic relationships, including frequent interspecies hybridization events, although strict reticulate evolutionary history could not be determined. All the population genetic parameters in the section Serpyllum suggest that the reproductive incompatibility between the species is very weak, which indicates a strong reticulating system, especially in the case when two or more species occur sympatrically. PB - Elsevier Gmbh, Munich T2 - Flora T1 - Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum) EP - 661 IS - 9 SP - 654 VL - 207 DO - 10.1016/j.flora.2012.06.018 ER -
@article{ author = "Šoštarić, Ivan and Liber, Zlatko and Grdisa, Martina and Marin, Petar D. and Dajić-Stevanović, Zora and Satović, Zlatko", year = "2012", abstract = "Amplified fragment length polymorphism (AFLP) markers were used to assess genetic relations of 32 Thymus populations belonging to seven species of genus Thymus, section Serpyllum, from Serbia. The collected species belong to three subsections: (1) Alternantes Klokov (Th. pulegioides L.), (2) Isolepides (Borbas) Halacsy (Th. glabrescens Wind.. Th. marschallianus Willd., Th. pannonicus All.), and (3) Pseudo-marginati (Braun ex Borbas) Jalas (Th. balcanus Borbas, Th. moesiacus Velen., Th. praecox Opiz). Analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to differences among individuals within populations, while the remaining variability was almost equally distributed among species and among populations within species. The pairwise AMOVA analyses further showed that species differentiation was significant in all cases, except between Th. marschallianus and Th. pannonicus. All the individuals belonging to Th. pulegioides (subsection Alternantes) formed a well-supported clade, the most divergent from all other species in this study. This genetic differentiation of Th. pulegioides is supported by its morphological features because it is the only analyzed taxon having hairs only on the stem edges, and can easily be recognized by this character. According to our results, the subsection Isolepides is polyphyletic. Th. glabrescens is clearly separated from the other two species of this subsection (Th. marschallianus, Th. pannonicus). Although morphologically distinguishable by leaf indumentum, individuals belonging to Th. marschallianus and Th. pannonicus could not be differentiated at the molecular level. The species belonging to subsection Pseudomarginati formed a monophyletic clade, although not supported by high bootstrap value. The clade is further divided into three well-supported clades representing distinct species (Th. balcanus, Th. praecox and Th. moesiacus). In our study, the AFLP markers demonstrated that they could be suitable for studying complex genetic relationships, including frequent interspecies hybridization events, although strict reticulate evolutionary history could not be determined. All the population genetic parameters in the section Serpyllum suggest that the reproductive incompatibility between the species is very weak, which indicates a strong reticulating system, especially in the case when two or more species occur sympatrically.", publisher = "Elsevier Gmbh, Munich", journal = "Flora", title = "Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)", pages = "661-654", number = "9", volume = "207", doi = "10.1016/j.flora.2012.06.018" }
Šoštarić, I., Liber, Z., Grdisa, M., Marin, P. D., Dajić-Stevanović, Z.,& Satović, Z.. (2012). Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum). in Flora Elsevier Gmbh, Munich., 207(9), 654-661. https://doi.org/10.1016/j.flora.2012.06.018
Šoštarić I, Liber Z, Grdisa M, Marin PD, Dajić-Stevanović Z, Satović Z. Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum). in Flora. 2012;207(9):654-661. doi:10.1016/j.flora.2012.06.018 .
Šoštarić, Ivan, Liber, Zlatko, Grdisa, Martina, Marin, Petar D., Dajić-Stevanović, Zora, Satović, Zlatko, "Genetic diversity and relationships among species of the genus Thymus L. (section Serpyllum)" in Flora, 207, no. 9 (2012):654-661, https://doi.org/10.1016/j.flora.2012.06.018 . .