TY  - CONF
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Karličič, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Golubović Ćurguz, Vesna
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6604
AB  - Organochlorinated pesticides (OCPs), such as DDT are characterized as highly persistent and mobile in environment, with high bioaccumulation capacity. The use of those chemicals has been banned in the United States and Europe for decades (Tsai, 2014). Despite this, OCPs-affected ecosystems are detected in several countries, even in the XXI century. Malusa et al. (2020) reported the presence of DDT in more than 80% of soil samples originating from organic farms in Poland. The objective was to determine the impact of microbial consortium on DDT biodegradation in soil. BacFung microbial consortium consisting of Bacillus sp., Pseudomonas sp., Azotobacter sp. and Trichoderma sp. was used for soil inoculation. Soil conditions were improved by grass sowing. The research was conducted at a playground in Tivat (Montenegro), and the size of the total examined area was 3,000 m2 divided into 13 sub-units. The concentration of DDT and its metabolites (DDE - dichlorodiphenyldichloroethylene and DDD - dichlorodiphenyldichloroethane) was measured by GC/MS technique. The initial DDT concentration in soil sub-units varied from 0.005 to 20.5 mg/kg. The sum of DDT+DDD+DDE (ΣDDT ) concentrations varied from 0.024 to 22.7 mg/kg (average concentration was 1.69 mg/kg). After the application of BacFung microbial consortium, the decline of DDT amount in soil sub-units was observed (from 0.005 to 0.057 mg/kg); ΣDDT was 0.020 to 0.2 mg/kg (average
value 0.055 mg/kg). The accumulation of DDD and DDE, as initial degradation products of DDT, was observed. These results showed that microbial consortium BacFung can be used in removal of DDT from polluted environment.
C3  - 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes
T1  - Microbial consortium in in situ remediation of DDT residues-affected soil
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6604
ER  - 

@conference{
author = "Kljujev, Igor and Lalević, Blažo and Karličič, Vera and Jovičić-Petrović, Jelena and Golubović Ćurguz, Vesna and Raičević, Vera",
year = "2023",
abstract = "Organochlorinated pesticides (OCPs), such as DDT are characterized as highly persistent and mobile in environment, with high bioaccumulation capacity. The use of those chemicals has been banned in the United States and Europe for decades (Tsai, 2014). Despite this, OCPs-affected ecosystems are detected in several countries, even in the XXI century. Malusa et al. (2020) reported the presence of DDT in more than 80% of soil samples originating from organic farms in Poland. The objective was to determine the impact of microbial consortium on DDT biodegradation in soil. BacFung microbial consortium consisting of Bacillus sp., Pseudomonas sp., Azotobacter sp. and Trichoderma sp. was used for soil inoculation. Soil conditions were improved by grass sowing. The research was conducted at a playground in Tivat (Montenegro), and the size of the total examined area was 3,000 m2 divided into 13 sub-units. The concentration of DDT and its metabolites (DDE - dichlorodiphenyldichloroethylene and DDD - dichlorodiphenyldichloroethane) was measured by GC/MS technique. The initial DDT concentration in soil sub-units varied from 0.005 to 20.5 mg/kg. The sum of DDT+DDD+DDE (ΣDDT ) concentrations varied from 0.024 to 22.7 mg/kg (average concentration was 1.69 mg/kg). After the application of BacFung microbial consortium, the decline of DDT amount in soil sub-units was observed (from 0.005 to 0.057 mg/kg); ΣDDT was 0.020 to 0.2 mg/kg (average
value 0.055 mg/kg). The accumulation of DDD and DDE, as initial degradation products of DDT, was observed. These results showed that microbial consortium BacFung can be used in removal of DDT from polluted environment.",
journal = "3rd International Meeting on New Strategies in Bioremediation/Restoration Processes",
title = "Microbial consortium in in situ remediation of DDT residues-affected soil",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6604"
}

Kljujev, I., Lalević, B., Karličič, V., Jovičić-Petrović, J., Golubović Ćurguz, V.,& Raičević, V.. (2023). Microbial consortium in in situ remediation of DDT residues-affected soil. in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes, 154.
https://hdl.handle.net/21.15107/rcub_agrospace_6604

Kljujev I, Lalević B, Karličič V, Jovičić-Petrović J, Golubović Ćurguz V, Raičević V. Microbial consortium in in situ remediation of DDT residues-affected soil. in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes. 2023;:154.
https://hdl.handle.net/21.15107/rcub_agrospace_6604 .

Kljujev, Igor, Lalević, Blažo, Karličič, Vera, Jovičić-Petrović, Jelena, Golubović Ćurguz, Vesna, Raičević, Vera, "Microbial consortium in in situ remediation of DDT residues-affected soil" in 3rd International Meeting on New Strategies in Bioremediation/Restoration Processes (2023):154,
https://hdl.handle.net/21.15107/rcub_agrospace_6604 .

TY  - JOUR
AU  - Đedović, Suzana
AU  - Stojanova, Monika
AU  - Bojkovski, Jovan
AU  - Kljujev, Igor
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6836
AB  - Microbial degradation, compared with many other degradation processes, is the most important
pathway for the depletion of triazine herbicides in soil. The aim of this study was to determine the
growth potential of Pseudomonas sp. CY in the presence of atrazine and additional carbon (sodium citrate) and nitrogen (ammonium-nitrate) sources. The experiment was performed with five treatments: i) 100 mg/L atrazine (control); ii) One hundred mg/L atrazine + sodium citrate (0.3 %,w/v); iii) One hundred mg/L atrazine + sodium citrate (0.3 %, w/v) + ammonium nitrate (0.6 %, w/v);iv) Atrazine (300 mg/L) + sodium citrate (0.3 %, w/v) and v) Atrazine (500 mg/L) + sodium citrate (0.3 %, w/v). The bacterial count was determined after incubation (7 days at 30°C) using the agar plate method, while atrazine degradation was determined by measuring the optical density at 221 nm.
Pseudomonas sp. CY can partially utilize atrazine as the sole source of carbon and energy. The
highest values of the bacterial count were determined at the highest initial atrazine concentrations; however, bacterial growth was not detected in these treatments. A significant impact of citrate on bacterial growth and atrazine degradation was observed, while the addition of nitrate decreased the atrazine degradation rate. This study confirmed that Pseudomonas sp. CY can be used as a prominent candidate for the remediation of atrazine-affected environments.
PB  - MDPI
T2  - ZEMLJISTE I BILJKA
T1  - Changes in Pseudomonas sp. CY growth in the presence of atrazine
DO  - 10.5937/ZemBilj2302001D
ER  - 

@article{
author = "Đedović, Suzana and Stojanova, Monika and Bojkovski, Jovan and Kljujev, Igor and Karličič, Vera and Lalević, Blažo and Raičević, Vera",
year = "2023",
abstract = "Microbial degradation, compared with many other degradation processes, is the most important
pathway for the depletion of triazine herbicides in soil. The aim of this study was to determine the
growth potential of Pseudomonas sp. CY in the presence of atrazine and additional carbon (sodium citrate) and nitrogen (ammonium-nitrate) sources. The experiment was performed with five treatments: i) 100 mg/L atrazine (control); ii) One hundred mg/L atrazine + sodium citrate (0.3 %,w/v); iii) One hundred mg/L atrazine + sodium citrate (0.3 %, w/v) + ammonium nitrate (0.6 %, w/v);iv) Atrazine (300 mg/L) + sodium citrate (0.3 %, w/v) and v) Atrazine (500 mg/L) + sodium citrate (0.3 %, w/v). The bacterial count was determined after incubation (7 days at 30°C) using the agar plate method, while atrazine degradation was determined by measuring the optical density at 221 nm.
Pseudomonas sp. CY can partially utilize atrazine as the sole source of carbon and energy. The
highest values of the bacterial count were determined at the highest initial atrazine concentrations; however, bacterial growth was not detected in these treatments. A significant impact of citrate on bacterial growth and atrazine degradation was observed, while the addition of nitrate decreased the atrazine degradation rate. This study confirmed that Pseudomonas sp. CY can be used as a prominent candidate for the remediation of atrazine-affected environments.",
publisher = "MDPI",
journal = "ZEMLJISTE I BILJKA",
title = "Changes in Pseudomonas sp. CY growth in the presence of atrazine",
doi = "10.5937/ZemBilj2302001D"
}

Đedović, S., Stojanova, M., Bojkovski, J., Kljujev, I., Karličič, V., Lalević, B.,& Raičević, V.. (2023). Changes in Pseudomonas sp. CY growth in the presence of atrazine. in ZEMLJISTE I BILJKA
MDPI..
https://doi.org/10.5937/ZemBilj2302001D

Đedović S, Stojanova M, Bojkovski J, Kljujev I, Karličič V, Lalević B, Raičević V. Changes in Pseudomonas sp. CY growth in the presence of atrazine. in ZEMLJISTE I BILJKA. 2023;.
doi:10.5937/ZemBilj2302001D .

Đedović, Suzana, Stojanova, Monika, Bojkovski, Jovan, Kljujev, Igor, Karličič, Vera, Lalević, Blažo, Raičević, Vera, "Changes in Pseudomonas sp. CY growth in the presence of atrazine" in ZEMLJISTE I BILJKA (2023),
https://doi.org/10.5937/ZemBilj2302001D . .

TY  - CONF
AU  - Karličič, Vera
AU  - Dragojević, Milica
AU  - Savić, Zagorka
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6606
AB  - Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.
C3  - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE
T1  - Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6606
ER  - 

@conference{
author = "Karličič, Vera and Dragojević, Milica and Savić, Zagorka and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2023",
abstract = "Modern agricultural practice is burdened with increasing caution related to
the use of chemical inputs in the form of fertilizers and pesticides. These concerns
have been followed by a growing interest in more ecological approaches
to nutrient supplementation and disease control management. As an option,
biofertilizers and biocontrol agents gather the scientific community, aiming to
deeply explore and emphasize “the solutions from nature”. In that context, the
objective of our research was to isolate Trichoderma spp. from different environments
(agricultural, forest soil, and pine bark) and test their biocontrol and
plant growth promoting potential. A total of 13 Trichoderma spp. were isolated,
and confrontation tests were established with Botrytis cinerea, Fusarium graminearum,
and Fusarium oxisporum. Biochemical characterization involved the
assessment of several plant growth promoting characteristics (production of
indoles, siderophores and enzymes). The confrontation test revealed different
mechanisms employed in newly formed interactions. The mycelia of six Trichoderma
isolates completely overgrew the tested pathogens, expressing very high
antagonistic activity (≥75%), and emphasizing the competitive character of the
isolates. The presence of an inhibition zone, a sign of antibiosis as a probable
mode of action, was recorded within F. oxisporum interactions with several Trichoderma
isolates. Biochemical characterization confirmed the production of
cell-wall degrading enzymes (cellulase, β-glucosidase and N-acetyl-β-glucosaminidase)
which are correlated to mycoparasitism. Biochemical assessments
confirmed the plant growth promoting nature of Trichoderma isolates, which
were capable of producing indoles, siderophores and enzymes involved in P
cycle (phosphatase and naphthol-AS-BI- phosphohydrolase). The obtained results
suggests several newly isolated Trichoderma spp. as effective plant growth
promotors capable of expressing direct and indirect mechanisms of growth
stimulation and acting as promising biocontrol agents against significant crop
pathogens. Further analyses demand assessment of field conditions performance
and deeper insight into the mechanisms involved in mutualistic interactions
with plants and antagonistic interactions with phytopathogens.",
journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE",
title = "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6606"
}

Karličič, V., Dragojević, M., Savić, Z., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2023). Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE.
https://hdl.handle.net/21.15107/rcub_agrospace_6606

Karličič V, Dragojević M, Savić Z, Jovičić-Petrović J, Kljujev I, Raičević V. Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

Karličič, Vera, Dragojević, Milica, Savić, Zagorka, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Trichoderma species: biofertilizers and biocontrol agents for agricultural sustainability" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6606 .

TY  - CONF
AU  - Karličić, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Lalević, Blažo
AU  - Raičević, Vera
AU  - Kljujev, Igor
AU  - Gudalović, Tatjana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6511
AB  - One of the major problems in agriculture are Fusarium species which cause fusariosis in wheat,
corn, tomato, melon, watermelon, onion, peas, and beans. Also, Fusarium can synthesize
thermostable mycotoxins which can lead to certain diseases if they were found in food.
Currently, chemical fungicides are mostly used to prevent the occurrence of fusariosis disease,
but the resistance of pathogens to such treatment is being more and more widespread. Soil
microorganisms help in plant defense and growth. The rhizosphere fungi have an important role
in the mutual exchange of nutrients with plants and they can establish specific interactions with
plants. Such rhizosphere fungi are known as plant growth-promoting (PGP). These PGP fungi
affect the better plant growth through the synthesis of certain phytohormones but they also have
the function as biocontrol agents. They can inhibit the growth of phytopathogens through direct
mechanisms of antibiosis, mycoparasitism, and competition.
The goal of this research was an investigation of the antagonistic effect of Trichoderma
harzianum TR1 and Trichoderma citrinoviride 1V on Fusarium oxysporum and Fusarium
graminearum. The antagonistic effect was examined through two tests: Dual culture test (DUAL
test) and the effect of volatile organic compounds (VOCs) of Trichoderma strains on the growth
of Fusarium strains. Also, the cell-wall degrading enzymatic activity of T. citrinoviride and T.
harzianum was examined. The results showed that antagonistic activity of Trichoderma strains
varies from moderate to high. Mycelial growth inhibition by T. citrinoviride was 44% for F.
graminearum and 67% for F. oxysporum. T. harzianum inhibited F. gramminearum growth for
59% and F. oxysporum for 66%. Based on the results, it was concluded that T.harzianum and
T.citrinoviride can be considered biocontrol agents for F. oxysporum and F. graminearum.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - ...
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
T1  - In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6511
ER  - 

@conference{
author = "Karličić, Vera and Jovičić-Petrović, Jelena and Lalević, Blažo and Raičević, Vera and Kljujev, Igor and Gudalović, Tatjana",
year = "2022",
abstract = "One of the major problems in agriculture are Fusarium species which cause fusariosis in wheat,
corn, tomato, melon, watermelon, onion, peas, and beans. Also, Fusarium can synthesize
thermostable mycotoxins which can lead to certain diseases if they were found in food.
Currently, chemical fungicides are mostly used to prevent the occurrence of fusariosis disease,
but the resistance of pathogens to such treatment is being more and more widespread. Soil
microorganisms help in plant defense and growth. The rhizosphere fungi have an important role
in the mutual exchange of nutrients with plants and they can establish specific interactions with
plants. Such rhizosphere fungi are known as plant growth-promoting (PGP). These PGP fungi
affect the better plant growth through the synthesis of certain phytohormones but they also have
the function as biocontrol agents. They can inhibit the growth of phytopathogens through direct
mechanisms of antibiosis, mycoparasitism, and competition.
The goal of this research was an investigation of the antagonistic effect of Trichoderma
harzianum TR1 and Trichoderma citrinoviride 1V on Fusarium oxysporum and Fusarium
graminearum. The antagonistic effect was examined through two tests: Dual culture test (DUAL
test) and the effect of volatile organic compounds (VOCs) of Trichoderma strains on the growth
of Fusarium strains. Also, the cell-wall degrading enzymatic activity of T. citrinoviride and T.
harzianum was examined. The results showed that antagonistic activity of Trichoderma strains
varies from moderate to high. Mycelial growth inhibition by T. citrinoviride was 44% for F.
graminearum and 67% for F. oxysporum. T. harzianum inhibited F. gramminearum growth for
59% and F. oxysporum for 66%. Based on the results, it was concluded that T.harzianum and
T.citrinoviride can be considered biocontrol agents for F. oxysporum and F. graminearum.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, ...",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”",
title = "In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6511"
}

Karličić, V., Jovičić-Petrović, J., Lalević, B., Raičević, V., Kljujev, I.,& Gudalović, T.. (2022). In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6511

Karličić V, Jovičić-Petrović J, Lalević B, Raičević V, Kljujev I, Gudalović T. In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6511 .

Karličić, Vera, Jovičić-Petrović, Jelena, Lalević, Blažo, Raičević, Vera, Kljujev, Igor, Gudalović, Tatjana, "In vitro antagonistic activity of Trichoerma spp. to Fusarium oxysporum and Fusarium graminearum" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6511 .

TY  - CONF
AU  - Karličič, Vera
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6603
AB  - Biopriming is a pre-sowing seed inoculation technique based on beneficial microorganisms. The aim of the study was to estimate biopriming potential of microbial consortium on soybean seeds. Obtained results showed a stimulative effect of biopriming resulting in 8% higher germination percentage. Inoculated seeds were characterised as more vigorous according to vigor I, vigor II and electrical conductivity results. Accelerated aging test decreased germination percentage of both treatments to approximately 66±2%. Inoculated seeds were marked as more vigorous according to vigor I index. Biopriming of soybean seeds resulted in an increase of germination parameters that suggest stimulative effects on seed quality.
C3  - 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia
T1  - Biopriming: multiple effects on soybean germination metrics
EP  - 282
SP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6603
ER  - 

@conference{
author = "Karličič, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena and Kljujev, Igor and Raičević, Vera",
year = "2022",
abstract = "Biopriming is a pre-sowing seed inoculation technique based on beneficial microorganisms. The aim of the study was to estimate biopriming potential of microbial consortium on soybean seeds. Obtained results showed a stimulative effect of biopriming resulting in 8% higher germination percentage. Inoculated seeds were characterised as more vigorous according to vigor I, vigor II and electrical conductivity results. Accelerated aging test decreased germination percentage of both treatments to approximately 66±2%. Inoculated seeds were marked as more vigorous according to vigor I index. Biopriming of soybean seeds resulted in an increase of germination parameters that suggest stimulative effects on seed quality.",
journal = "57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia",
title = "Biopriming: multiple effects on soybean germination metrics",
pages = "282-278",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6603"
}

Karličič, V., Lalević, B., Jovičić-Petrović, J., Kljujev, I.,& Raičević, V.. (2022). Biopriming: multiple effects on soybean germination metrics. in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia, 278-282.
https://hdl.handle.net/21.15107/rcub_agrospace_6603

Karličič V, Lalević B, Jovičić-Petrović J, Kljujev I, Raičević V. Biopriming: multiple effects on soybean germination metrics. in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia. 2022;:278-282.
https://hdl.handle.net/21.15107/rcub_agrospace_6603 .

Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, Kljujev, Igor, Raičević, Vera, "Biopriming: multiple effects on soybean germination metrics" in 57th Croatian & 17th Internaonal Symposium on Agriculture, Vodice, Croatia (2022):278-282,
https://hdl.handle.net/21.15107/rcub_agrospace_6603 .

Raičević, V., Karličič, V., Lalević, B., Jovičić-Petrović, J., Kljujev, I.,& Golubović Ćurguz, V.. (2022). Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola. 
University of Belgrade, Faculty of Agriculture, Serbia..
https://hdl.handle.net/21.15107/rcub_agrospace_6723

Raičević V, Karličič V, Lalević B, Jovičić-Petrović J, Kljujev I, Golubović Ćurguz V. Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6723 .

Raičević, Vera, Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, Kljujev, Igor, Golubović Ćurguz, Vesna, "Konzorcijum bakterija stimulatora biljnog rasta u revegetaciji deposola" (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6723 .

TY  - GEN
AU  - Karličič, Vera
AU  - Simić, Aleksandar
AU  - Brajević, Snežana
AU  - Kljujev, Igor
AU  - Jovičić-Petrović, Jelena
AU  - Raičević, Vera
AU  - Lalević, Blažo
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6724
AB  - Eksploatacija rude, i pored benefita za ljudsko društvo, dovodi do pogoršanja kvaliteta životne sredine. Kao posledica eksploatacije, dolazi do deponovanja otpadnog materijala i stvaranja jalovina sa nepovoljnim fizičkim, hemijskim i biološkim osobinama. Imajući u vidu značaj mikroorganizama u oštećenim ekosistemima, cilj ovog rada bio je ispitivanje mikrobnog diverziteta jalovine; na jednom delu jalovine izvršena je setva semena crvenog vijuka uz dodatak organskog i mineralnog đubriva (CVF), na drugom setva istog semena bez đubrenja (CV), dok je treći deo, bez setve i đubrenja, predstavljao kontrolu (KON). Mikrobni diverzitet ispitan je metodom agarnih ploča, pri čemu je određen ukupan broj bakterija, amonifikatora i gljiva, kao i prisustvo vrsta iz rodova Pseudomonas sp. i Azotobacter sp. Dobijeni rezultati ukazuju na nizak ukupan broj bakterija, amonifikatora i gljiva, dok vrste iz rodova Pseudomonas sp. i Azotobacter sp. nisu detektovane. Slaba zastupljenost mikrobnih populacija je posledica niskog sadržaja hranljivih supstanci i visoke koncentracije teških metala. Đubrenje je uticalo na povećanje mikrobiološke aktivnosti, kao i na promenu odnosa fungalnih i bakterijskih populacija, što ukazuje na oporavak oštećenog ekosistema.
T2  - XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac
T1  - Mikrobni diverzitet kao pokazatelj remedijacije jalovine
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6724
ER  - 

@misc{
author = "Karličič, Vera and Simić, Aleksandar and Brajević, Snežana and Kljujev, Igor and Jovičić-Petrović, Jelena and Raičević, Vera and Lalević, Blažo",
year = "2022",
abstract = "Eksploatacija rude, i pored benefita za ljudsko društvo, dovodi do pogoršanja kvaliteta životne sredine. Kao posledica eksploatacije, dolazi do deponovanja otpadnog materijala i stvaranja jalovina sa nepovoljnim fizičkim, hemijskim i biološkim osobinama. Imajući u vidu značaj mikroorganizama u oštećenim ekosistemima, cilj ovog rada bio je ispitivanje mikrobnog diverziteta jalovine; na jednom delu jalovine izvršena je setva semena crvenog vijuka uz dodatak organskog i mineralnog đubriva (CVF), na drugom setva istog semena bez đubrenja (CV), dok je treći deo, bez setve i đubrenja, predstavljao kontrolu (KON). Mikrobni diverzitet ispitan je metodom agarnih ploča, pri čemu je određen ukupan broj bakterija, amonifikatora i gljiva, kao i prisustvo vrsta iz rodova Pseudomonas sp. i Azotobacter sp. Dobijeni rezultati ukazuju na nizak ukupan broj bakterija, amonifikatora i gljiva, dok vrste iz rodova Pseudomonas sp. i Azotobacter sp. nisu detektovane. Slaba zastupljenost mikrobnih populacija je posledica niskog sadržaja hranljivih supstanci i visoke koncentracije teških metala. Đubrenje je uticalo na povećanje mikrobiološke aktivnosti, kao i na promenu odnosa fungalnih i bakterijskih populacija, što ukazuje na oporavak oštećenog ekosistema.",
journal = "XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac",
title = "Mikrobni diverzitet kao pokazatelj remedijacije jalovine",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6724"
}

Karličič, V., Simić, A., Brajević, S., Kljujev, I., Jovičić-Petrović, J., Raičević, V.,& Lalević, B.. (2022). Mikrobni diverzitet kao pokazatelj remedijacije jalovine. in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac.
https://hdl.handle.net/21.15107/rcub_agrospace_6724

Karličič V, Simić A, Brajević S, Kljujev I, Jovičić-Petrović J, Raičević V, Lalević B. Mikrobni diverzitet kao pokazatelj remedijacije jalovine. in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6724 .

Karličič, Vera, Simić, Aleksandar, Brajević, Snežana, Kljujev, Igor, Jovičić-Petrović, Jelena, Raičević, Vera, Lalević, Blažo, "Mikrobni diverzitet kao pokazatelj remedijacije jalovine" in XVI Savetovanje Održivi razvoj Braničevskog okruga i energetskog kompleksa Kostolac (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6724 .

TY  - JOUR
AU  - Radić, Danka
AU  - Karličić, Vera
AU  - Đorđević, Jelena
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6116
AB  - A large number of soil microorganisms are characterized as plant growth promoting, but there seems to be a lack of comprehensive knowledge regarding plant growth promoting soil yeasts. The aim of the experiment was to analyse the properties of three yeast species: Schwanniomyces occidentalis BK0302D, Cyberlindnera saturnus CK2404I and Candida tropicalis 2TD2912B, important for plant growth (ammonium sulphate transformation, phosphorus, potassium and zinc dissolution), and to evaluate the effect of yeast on the growth of common wheat and white mustard seedlings after seeds’ inoculation. Common wheat and white mustard seeds were inoculated with the selected yeasts. The final measurements showed that the highest amount of nitrate (10.40 μg mL-1 NO3 −) was produced by C. saturnus CK2404I, while S. occidentalis BK0302D solubilized the largest amount of phosphorus (63.70 μg mL-1 P). All three strains are marked as potassium and zinc solubilizers with both acid and alkaline phosphatase activity. This is the first report on S. occidentalis and C. tropicalis ability to solubilize insoluble potassium and zinc, and C. saturnus ability to solubilize insoluble phosphorus, potassium and zinc. Also, C. tropicalis 2TD2912B exhibited high antagonistic activity (66% growth inhibition) toward Botrytis cinerea. In vivo trial was conducted in a low-nutrient substrate, and S. occidentalis BK0302D was found to have the most considerable influence on common wheat biomass production (34% increase). White mustard inoculation with C. saturnus CK2404I resulted in a 4-fold higher biomass production, while S. occidentalis BK0302D induced a 2-fold increase. The presented results confirmed the multi-functional plant growth promoting characteristics of the tested yeasts and their potential for broad application from conventional agriculture on low-nutrient soils to revegetation of disturbed substrates. © 2022, Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University. All rights reserved.
T2  - Zemdirbyste
T2  - Zemdirbyste
T1  - Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard
EP  - 34
IS  - 1
SP  - 27
VL  - 109
DO  - 10.13080/Z-A.2022.109.004
ER  - 

@article{
author = "Radić, Danka and Karličić, Vera and Đorđević, Jelena and Jovičić-Petrović, Jelena and Kljujev, Igor and Lalević, Blažo and Raičević, Vera",
year = "2022",
abstract = "A large number of soil microorganisms are characterized as plant growth promoting, but there seems to be a lack of comprehensive knowledge regarding plant growth promoting soil yeasts. The aim of the experiment was to analyse the properties of three yeast species: Schwanniomyces occidentalis BK0302D, Cyberlindnera saturnus CK2404I and Candida tropicalis 2TD2912B, important for plant growth (ammonium sulphate transformation, phosphorus, potassium and zinc dissolution), and to evaluate the effect of yeast on the growth of common wheat and white mustard seedlings after seeds’ inoculation. Common wheat and white mustard seeds were inoculated with the selected yeasts. The final measurements showed that the highest amount of nitrate (10.40 μg mL-1 NO3 −) was produced by C. saturnus CK2404I, while S. occidentalis BK0302D solubilized the largest amount of phosphorus (63.70 μg mL-1 P). All three strains are marked as potassium and zinc solubilizers with both acid and alkaline phosphatase activity. This is the first report on S. occidentalis and C. tropicalis ability to solubilize insoluble potassium and zinc, and C. saturnus ability to solubilize insoluble phosphorus, potassium and zinc. Also, C. tropicalis 2TD2912B exhibited high antagonistic activity (66% growth inhibition) toward Botrytis cinerea. In vivo trial was conducted in a low-nutrient substrate, and S. occidentalis BK0302D was found to have the most considerable influence on common wheat biomass production (34% increase). White mustard inoculation with C. saturnus CK2404I resulted in a 4-fold higher biomass production, while S. occidentalis BK0302D induced a 2-fold increase. The presented results confirmed the multi-functional plant growth promoting characteristics of the tested yeasts and their potential for broad application from conventional agriculture on low-nutrient soils to revegetation of disturbed substrates. © 2022, Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University. All rights reserved.",
journal = "Zemdirbyste, Zemdirbyste",
title = "Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard",
pages = "34-27",
number = "1",
volume = "109",
doi = "10.13080/Z-A.2022.109.004"
}

Radić, D., Karličić, V., Đorđević, J., Jovičić-Petrović, J., Kljujev, I., Lalević, B.,& Raičević, V.. (2022). Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard. in Zemdirbyste, 109(1), 27-34.
https://doi.org/10.13080/Z-A.2022.109.004

Radić D, Karličić V, Đorđević J, Jovičić-Petrović J, Kljujev I, Lalević B, Raičević V. Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard. in Zemdirbyste. 2022;109(1):27-34.
doi:10.13080/Z-A.2022.109.004 .

Radić, Danka, Karličić, Vera, Đorđević, Jelena, Jovičić-Petrović, Jelena, Kljujev, Igor, Lalević, Blažo, Raičević, Vera, "Soil yeasts promoting plant growth: Benefits for the development of common wheat and white mustard" in Zemdirbyste, 109, no. 1 (2022):27-34,
https://doi.org/10.13080/Z-A.2022.109.004 . .

TY  - JOUR
AU  - Kerečki, Slavica
AU  - Pećinar, Ilinka
AU  - Karličić, Vera
AU  - Mirković, Nemanja
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6143
AB  - This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
T2  - Journal of Plant Interactions
T2  - Journal of Plant Interactions
T1  - Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming
EP  - 730
IS  - 1
SP  - 719
VL  - 17
DO  - 10.1080/17429145.2022.2091802
ER  - 

@article{
author = "Kerečki, Slavica and Pećinar, Ilinka and Karličić, Vera and Mirković, Nemanja and Kljujev, Igor and Raičević, Vera and Jovičić-Petrović, Jelena",
year = "2022",
abstract = "This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",
journal = "Journal of Plant Interactions, Journal of Plant Interactions",
title = "Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming",
pages = "730-719",
number = "1",
volume = "17",
doi = "10.1080/17429145.2022.2091802"
}

Kerečki, S., Pećinar, I., Karličić, V., Mirković, N., Kljujev, I., Raičević, V.,& Jovičić-Petrović, J.. (2022). Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming. in Journal of Plant Interactions, 17(1), 719-730.
https://doi.org/10.1080/17429145.2022.2091802

Kerečki S, Pećinar I, Karličić V, Mirković N, Kljujev I, Raičević V, Jovičić-Petrović J. Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming. in Journal of Plant Interactions. 2022;17(1):719-730.
doi:10.1080/17429145.2022.2091802 .

Kerečki, Slavica, Pećinar, Ilinka, Karličić, Vera, Mirković, Nemanja, Kljujev, Igor, Raičević, Vera, Jovičić-Petrović, Jelena, "Azotobacter chroococcum F8/2: a multitasking
bacterial strain in sugar beet biopriming" in Journal of Plant Interactions, 17, no. 1 (2022):719-730,
https://doi.org/10.1080/17429145.2022.2091802 . .

TY  - CONF
AU  - Kerečki, Slavica
AU  - Jovičić-Petrović, Jelena
AU  - Karličić, Vera
AU  - Kljujev, Igor
AU  - Ćirković, Saša
AU  - Ristić-Đurović, Jasna
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6512
AB  - Seed inoculation (biopriming) represents an agronomic practice directed towards improving
germination, as well as fostering beneficial plant-microbe interaction from the very beginning
of plants’ life. Besides biopriming, static magnetic field (SMF) is studied as an abiotic factor
affecting germination and plant growth. This paper is aimed to examine the combined effect
of Azotobacter chroococcum F8/2 and SMF of 90 mT on germination. A. chroococcum F8/2
has been proven as a successful biopriming agent, with beneficial effect on cucumber,
tomato, wheat, and soybean germination. This research starts from the hypothesis that the
combined effect of Azotobacter inoculation and SMF could lead to synergistic improvement
of germination parameters, compared to already shown effects of biopriming itself. The
research was conducted with following cultivable plants: basil, cucumber, tomato, wheat, and
soybean. Seed treatment was performed by 1h-immersion of surface-sterilized seeds into
bacterial suspension (107CFU/ml), followed by exposure to SMF of 90 mT for 5 min and 15
min. The germination test was conducted with 100 seeds per treatment and lasted 7 days. The
highest improvement of germination percentages was observed in cucumber and basil (an
increase for 35-41% and 41-45%, respectively), compared to biopriming without SMF
treatment. Tomato and wheat germination were not improved by addition of SMF treatment
to biopriming. The obtained results indicate that the application of SMF can affect the
germination parameters that are changed by biopriming. There is a need for further research
in order to explain the differences between plant species’ response.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - ...
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
T1  - Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6512
ER  - 

@conference{
author = "Kerečki, Slavica and Jovičić-Petrović, Jelena and Karličić, Vera and Kljujev, Igor and Ćirković, Saša and Ristić-Đurović, Jasna",
year = "2022",
abstract = "Seed inoculation (biopriming) represents an agronomic practice directed towards improving
germination, as well as fostering beneficial plant-microbe interaction from the very beginning
of plants’ life. Besides biopriming, static magnetic field (SMF) is studied as an abiotic factor
affecting germination and plant growth. This paper is aimed to examine the combined effect
of Azotobacter chroococcum F8/2 and SMF of 90 mT on germination. A. chroococcum F8/2
has been proven as a successful biopriming agent, with beneficial effect on cucumber,
tomato, wheat, and soybean germination. This research starts from the hypothesis that the
combined effect of Azotobacter inoculation and SMF could lead to synergistic improvement
of germination parameters, compared to already shown effects of biopriming itself. The
research was conducted with following cultivable plants: basil, cucumber, tomato, wheat, and
soybean. Seed treatment was performed by 1h-immersion of surface-sterilized seeds into
bacterial suspension (107CFU/ml), followed by exposure to SMF of 90 mT for 5 min and 15
min. The germination test was conducted with 100 seeds per treatment and lasted 7 days. The
highest improvement of germination percentages was observed in cucumber and basil (an
increase for 35-41% and 41-45%, respectively), compared to biopriming without SMF
treatment. Tomato and wheat germination were not improved by addition of SMF treatment
to biopriming. The obtained results indicate that the application of SMF can affect the
germination parameters that are changed by biopriming. There is a need for further research
in order to explain the differences between plant species’ response.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, ...",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”",
title = "Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6512"
}

Kerečki, S., Jovičić-Petrović, J., Karličić, V., Kljujev, I., Ćirković, S.,& Ristić-Đurović, J.. (2022). Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6512

Kerečki S, Jovičić-Petrović J, Karličić V, Kljujev I, Ćirković S, Ristić-Đurović J. Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022”. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6512 .

Kerečki, Slavica, Jovičić-Petrović, Jelena, Karličić, Vera, Kljujev, Igor, Ćirković, Saša, Ristić-Đurović, Jasna, "Static magnetic field improves effects of biopriming by Azotobacter chroococcum F8/2" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6512 .

TY  - CONF
AU  - Kljujev, Igor
AU  - Jovicic-Petrovic, Jelena
AU  - Lalevic, Blazo
AU  - Karlicic, Vera
AU  - Todorovic, Irena
AU  - Prijepoljac, Minela
AU  - Raicevic, Vera
PY  - 2021
UR  - https://iwa-network.org/events/12th-eastern-european-young-water-professionals-conference-water-for-all-water-for-nature-reliable-water-supply-wastewater-treatment/#:~:text=In%202021%20we%20organize%20our,it%20will%20not%20be%20possible.
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6577
AB  - Surface water contamination becomes a raising problem due to different anthropogenic factors such as agriculture, industry, waste landfills, and wastewater effluents. Small rivers are also exposed to wastewater influents, pollution from agricultural activities, as well as raising human influence on the river flow (dam building). This research aimed to analyze the microbiological quality and ecological status of the water on the small river Ribnica in Western Serbia. Ribnica is a 22 km long tributary of Kolubara. The upper part of the river flow is surrounded by forests, agricultural fields, and small farms. Downstream, the river flows through Mionica, a small town where its water quality becomes endangered by communal wastewater, as well as landfills in the river surrounding. The microbiological quality of river water was estimated by the presence of coliforms, E. coli, enterococci which determination was done by Colilert and Enterolert method (IDEXX Laboratories, USA). The other parameters for water ecological status (oligotrophs, heterotrophs microorganisms, and autopurification index) are determined using the serial dilution method and incubation on selective nutrient media. The index of autopurification was calculated as oligotrophs/heterotrophs ratio. Our results show that the greatest pollution comes in the river after Mionica center. The extremely large number of fecal E. coli indicates the presence of fecal matter in water which could be a potential risk for human health if this water is used for irrigation crops or recreation. Also, autopurification index of water in this location indicates very poor self-purification potential which means that this water needs human help for achieving good ecological status. Regular monitoring and analyses of microbiological contamination and ecosystem disturbances should be important input data for public policies and strategic planning in the area of wastewater management, hydraulic engineering, and agricultural management.
C3  - 12th Eastern European Young Water Professionals Conference IWA
T1  - Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6577
ER  - 

@conference{
author = "Kljujev, Igor and Jovicic-Petrovic, Jelena and Lalevic, Blazo and Karlicic, Vera and Todorovic, Irena and Prijepoljac, Minela and Raicevic, Vera",
year = "2021",
abstract = "Surface water contamination becomes a raising problem due to different anthropogenic factors such as agriculture, industry, waste landfills, and wastewater effluents. Small rivers are also exposed to wastewater influents, pollution from agricultural activities, as well as raising human influence on the river flow (dam building). This research aimed to analyze the microbiological quality and ecological status of the water on the small river Ribnica in Western Serbia. Ribnica is a 22 km long tributary of Kolubara. The upper part of the river flow is surrounded by forests, agricultural fields, and small farms. Downstream, the river flows through Mionica, a small town where its water quality becomes endangered by communal wastewater, as well as landfills in the river surrounding. The microbiological quality of river water was estimated by the presence of coliforms, E. coli, enterococci which determination was done by Colilert and Enterolert method (IDEXX Laboratories, USA). The other parameters for water ecological status (oligotrophs, heterotrophs microorganisms, and autopurification index) are determined using the serial dilution method and incubation on selective nutrient media. The index of autopurification was calculated as oligotrophs/heterotrophs ratio. Our results show that the greatest pollution comes in the river after Mionica center. The extremely large number of fecal E. coli indicates the presence of fecal matter in water which could be a potential risk for human health if this water is used for irrigation crops or recreation. Also, autopurification index of water in this location indicates very poor self-purification potential which means that this water needs human help for achieving good ecological status. Regular monitoring and analyses of microbiological contamination and ecosystem disturbances should be important input data for public policies and strategic planning in the area of wastewater management, hydraulic engineering, and agricultural management.",
journal = "12th Eastern European Young Water Professionals Conference IWA",
title = "Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6577"
}

Kljujev, I., Jovicic-Petrovic, J., Lalevic, B., Karlicic, V., Todorovic, I., Prijepoljac, M.,& Raicevic, V.. (2021). Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water. in 12th Eastern European Young Water Professionals Conference IWA.
https://hdl.handle.net/21.15107/rcub_agrospace_6577

Kljujev I, Jovicic-Petrovic J, Lalevic B, Karlicic V, Todorovic I, Prijepoljac M, Raicevic V. Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water. in 12th Eastern European Young Water Professionals Conference IWA. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6577 .

Kljujev, Igor, Jovicic-Petrovic, Jelena, Lalevic, Blazo, Karlicic, Vera, Todorovic, Irena, Prijepoljac, Minela, Raicevic, Vera, "Microbiological Quality, Ecological Status, and Potential Sources of Contamination of the River Water" in 12th Eastern European Young Water Professionals Conference IWA (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6577 .

TY  - CONF
AU  - Kerečki, Slavica
AU  - Jovičić-Petrović, Jelena
AU  - Kljujev, Igor
AU  - Lalević, Blažo
AU  - Karličič, Vera
AU  - Petrović, Ivana
AU  - Raičević, Vera
PY  - 2021
UR  - https://agrosym.ues.rs.ba/article/showpdf/BOOK_OF_PROCEEDINGS_2021_FINAL.pdf
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6578
AB  - Crop yields are highly depended on germination and early stages of plant growth. Numerous
priming techniques are being developed aimed to improve germination. Biopriming represents a
sustainable approach based on seed treatment in bacterial suspension of selected plant growth
promoting strains. One of the most promising plant growth promoting bacteria is Azotobacter
chroococcum. The aim of the research was to evaluate the effects of A. chroococcum F8/2 as a
biopriming agent on germination of various cultivable plants: basil, white mustard, cucumber,
tomato, wheat, canola, and soybean. After surface sterilization, seeds were bioprimed in the
bacterial suspension (107CFU/ml). Uninoculated seeds represented control treatment.
Germination test was conducted with 100 seeds per treatment and the germination was
monitored for 7 days. Following germination parameters were determined: germination
percentage, germination index, mean germination time, vigor I, vigor II, length and dry biomass
of the seedlings. The bacterial inoculation caused higher germination percentages of cucumber,
tomato, wheat and soybean. The highest increase in germination index was observed in wheat
(an increase of 19.8%). Tomato and basil were the only plants where vigor I was not increased
by inoculation. Generally, the most favorable effects of A. chroococcum biopriming were
observed in wheat where vigor I was increased more than twice, and vigor II was higher by
75.4% in inoculated seeds. The results indicate a significant potential for A. chroococcum use in
biopriming. The observed effects of seed priming on germination parameters were crop-specific,
with the most prominent potential in wheat biopriming.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
PB  - University of Belgrade, Faculty of Agriculture, Serbia
PB  - Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy
PB  - International Society of Environment and Rural Development, Japan
PB  - ...
C3  - Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
T1  - BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6578
ER  - 

@conference{
author = "Kerečki, Slavica and Jovičić-Petrović, Jelena and Kljujev, Igor and Lalević, Blažo and Karličič, Vera and Petrović, Ivana and Raičević, Vera",
year = "2021",
abstract = "Crop yields are highly depended on germination and early stages of plant growth. Numerous
priming techniques are being developed aimed to improve germination. Biopriming represents a
sustainable approach based on seed treatment in bacterial suspension of selected plant growth
promoting strains. One of the most promising plant growth promoting bacteria is Azotobacter
chroococcum. The aim of the research was to evaluate the effects of A. chroococcum F8/2 as a
biopriming agent on germination of various cultivable plants: basil, white mustard, cucumber,
tomato, wheat, canola, and soybean. After surface sterilization, seeds were bioprimed in the
bacterial suspension (107CFU/ml). Uninoculated seeds represented control treatment.
Germination test was conducted with 100 seeds per treatment and the germination was
monitored for 7 days. Following germination parameters were determined: germination
percentage, germination index, mean germination time, vigor I, vigor II, length and dry biomass
of the seedlings. The bacterial inoculation caused higher germination percentages of cucumber,
tomato, wheat and soybean. The highest increase in germination index was observed in wheat
(an increase of 19.8%). Tomato and basil were the only plants where vigor I was not increased
by inoculation. Generally, the most favorable effects of A. chroococcum biopriming were
observed in wheat where vigor I was increased more than twice, and vigor II was higher by
75.4% in inoculated seeds. The results indicate a significant potential for A. chroococcum use in
biopriming. The observed effects of seed priming on germination parameters were crop-specific,
with the most prominent potential in wheat biopriming.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, University of Belgrade, Faculty of Agriculture, Serbia, Mediterranean Agronomic Institute of Bari (CIHEAM - IAMB) Italy, International Society of Environment and Rural Development, Japan, ...",
journal = "Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”",
title = "BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6578"
}

Kerečki, S., Jovičić-Petrović, J., Kljujev, I., Lalević, B., Karličič, V., Petrović, I.,& Raičević, V.. (2021). BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia..
https://hdl.handle.net/21.15107/rcub_agrospace_6578

Kerečki S, Jovičić-Petrović J, Kljujev I, Lalević B, Karličič V, Petrović I, Raičević V. BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6578 .

Kerečki, Slavica, Jovičić-Petrović, Jelena, Kljujev, Igor, Lalević, Blažo, Karličič, Vera, Petrović, Ivana, Raičević, Vera, "BIOPRIMING: A SUSTAINABLE SUPPORT FOR CROP ESTABLISHMENT" in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021” (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6578 .

TY  - CONF
AU  - Kljujev, Igor
AU  - Karličič, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Veličković, Ana
AU  - Lalević, Blažo
AU  - Raičević, Vera
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6602
AB  - Good microbiological quality of irrigation water has a great importance for health-safety
agricultural production. In order to avoid microbiological contamination of crops, the
microbiological quality of the water supplying the crops should be monitored. The aim of this
paper is investigation of the microbiological quality of the water from 5 channels and one pool
from the municipality of Surcin which can potentially be used for irrigation, as well as
determination the microbiological quality of the vegetables which are irrigated from the pool.
Microbiological parameters of water and vegetables quality are determined by the standard
methods prescribed by the Rulebook on parameters of surface waters (MPN method) and
Rulebook of food hygiene of production of Republic of Serbia. The values of the tested
parameters in one of the five investigated channels, Surcin channel water, were higher in relation
to the limit values of the Rulebook. The increased levels of total and fecal coliforms, Escherichia
coli, Salmonella, and Shigella were found. The Surcin channel water requires appropriate
treatment before its use for crop irrigation. The values of other tested waters did not go beyond
the limit values prescribed by the Rulebook, but for the safer agricultural production their
treatment is recommended. Microbiological analyzes of vegetables were also done. The total and
fecal coliform bacteria, as well as E. coli, Salmonella sp. and Shigella sp., were not detected in
the tested vegetable samples.
C3  - Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”
T1  - MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION
EP  - 529
SP  - 523
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6602
ER  - 

@conference{
author = "Kljujev, Igor and Karličič, Vera and Jovičić-Petrović, Jelena and Veličković, Ana and Lalević, Blažo and Raičević, Vera",
year = "2021",
abstract = "Good microbiological quality of irrigation water has a great importance for health-safety
agricultural production. In order to avoid microbiological contamination of crops, the
microbiological quality of the water supplying the crops should be monitored. The aim of this
paper is investigation of the microbiological quality of the water from 5 channels and one pool
from the municipality of Surcin which can potentially be used for irrigation, as well as
determination the microbiological quality of the vegetables which are irrigated from the pool.
Microbiological parameters of water and vegetables quality are determined by the standard
methods prescribed by the Rulebook on parameters of surface waters (MPN method) and
Rulebook of food hygiene of production of Republic of Serbia. The values of the tested
parameters in one of the five investigated channels, Surcin channel water, were higher in relation
to the limit values of the Rulebook. The increased levels of total and fecal coliforms, Escherichia
coli, Salmonella, and Shigella were found. The Surcin channel water requires appropriate
treatment before its use for crop irrigation. The values of other tested waters did not go beyond
the limit values prescribed by the Rulebook, but for the safer agricultural production their
treatment is recommended. Microbiological analyzes of vegetables were also done. The total and
fecal coliform bacteria, as well as E. coli, Salmonella sp. and Shigella sp., were not detected in
the tested vegetable samples.",
journal = "Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”",
title = "MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION",
pages = "529-523",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6602"
}

Kljujev, I., Karličič, V., Jovičić-Petrović, J., Veličković, A., Lalević, B.,& Raičević, V.. (2021). MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, 523-529.
https://hdl.handle.net/21.15107/rcub_agrospace_6602

Kljujev I, Karličič V, Jovičić-Petrović J, Veličković A, Lalević B, Raičević V. MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”. 2021;:523-529.
https://hdl.handle.net/21.15107/rcub_agrospace_6602 .

Kljujev, Igor, Karličič, Vera, Jovičić-Petrović, Jelena, Veličković, Ana, Lalević, Blažo, Raičević, Vera, "MICROBIOLOGICAL QUALITY OF SURFACE WATER AND SAFE VEGETABLE PRODUCTION" in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021” (2021):523-529,
https://hdl.handle.net/21.15107/rcub_agrospace_6602 .

TY  - JOUR
AU  - Milinković, Mira
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
AU  - Golubović-Ćurguz, Vesna
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5075
AB  - Besides ecological and environmental benefits of green open spaces, horticultural waste management has various environmental consequences. Green waste composting represents promising environmentally friendly alternative which gives valuable products with positive soil and plants impact. Composting products' quality determinates their application and depends on the particular waste material and process parameters. The aim of this paper was to estimate the chemical and microbiological quality of green waste compost and compost products (compost tea, compost extract and the solid phase after extraction), and their biopotential based on germination rate, germination index and inhibition of phytopathogenic fungi growth. Higher germination rate of examined plant seeds was noticed on the solid phase after extraction, and compost extract, compared to compost, and compost tea, respectively. Plants with low germination rate grown on compost showed higher fresh and dry biomass. Compost products strongly inhibited the growth of plant pathogens Fusarium oxysporum, Rhizoctonia sp., and Pythium debaryanum. Presented results show that composted biodegradable waste from urban green spaces contribute to the plant growth and phytopathogenic fungi suppression, and thus improve the overall environmental quality.
PB  - Inst Chemical Engineers, Rugby
T2  - Process Safety and Environmental Protection
T1  - Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression
EP  - 306
SP  - 299
VL  - 121
DO  - 10.1016/j.psep.2018.09.024
ER  - 

@article{
author = "Milinković, Mira and Lalević, Blažo and Jovičić-Petrović, Jelena and Golubović-Ćurguz, Vesna and Kljujev, Igor and Raičević, Vera",
year = "2019",
abstract = "Besides ecological and environmental benefits of green open spaces, horticultural waste management has various environmental consequences. Green waste composting represents promising environmentally friendly alternative which gives valuable products with positive soil and plants impact. Composting products' quality determinates their application and depends on the particular waste material and process parameters. The aim of this paper was to estimate the chemical and microbiological quality of green waste compost and compost products (compost tea, compost extract and the solid phase after extraction), and their biopotential based on germination rate, germination index and inhibition of phytopathogenic fungi growth. Higher germination rate of examined plant seeds was noticed on the solid phase after extraction, and compost extract, compared to compost, and compost tea, respectively. Plants with low germination rate grown on compost showed higher fresh and dry biomass. Compost products strongly inhibited the growth of plant pathogens Fusarium oxysporum, Rhizoctonia sp., and Pythium debaryanum. Presented results show that composted biodegradable waste from urban green spaces contribute to the plant growth and phytopathogenic fungi suppression, and thus improve the overall environmental quality.",
publisher = "Inst Chemical Engineers, Rugby",
journal = "Process Safety and Environmental Protection",
title = "Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression",
pages = "306-299",
volume = "121",
doi = "10.1016/j.psep.2018.09.024"
}

Milinković, M., Lalević, B., Jovičić-Petrović, J., Golubović-Ćurguz, V., Kljujev, I.,& Raičević, V.. (2019). Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression. in Process Safety and Environmental Protection
Inst Chemical Engineers, Rugby., 121, 299-306.
https://doi.org/10.1016/j.psep.2018.09.024

Milinković M, Lalević B, Jovičić-Petrović J, Golubović-Ćurguz V, Kljujev I, Raičević V. Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression. in Process Safety and Environmental Protection. 2019;121:299-306.
doi:10.1016/j.psep.2018.09.024 .

Milinković, Mira, Lalević, Blažo, Jovičić-Petrović, Jelena, Golubović-Ćurguz, Vesna, Kljujev, Igor, Raičević, Vera, "Biopotential of compost and compost products derived from horticultural waste-Effect on plant growth and plant pathogens' suppression" in Process Safety and Environmental Protection, 121 (2019):299-306,
https://doi.org/10.1016/j.psep.2018.09.024 . .

TY  - CONF
AU  - Vujović, Bojana
AU  - Jovičić-Petrović, Jelena
AU  - Rašković, Nikola
AU  - Vukmanović, Tamara
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6844
AB  - Podzemne vode predstavljaju najočuvaniji deo hidrosfere, zaštićen od globalne
degradacije kvaliteta vode, zbog čega je široko rasprostranjen i često korišćen resurs
vodosnabdevanja. Iako se podzemne vode smatraju mikrobiološki slabo naseljenim
delom biosfere, transformacija organskog ugljenika, transformacija azota, gvožđa i
sumpora između oksidovanog i redukovanog stanja, produkcija metana i drugi
fundamentalni procesi se i u podzemnim vodama odigravaju mikrobiološkom
aktivnošću.1 Iako bakterije koje pripadaju različitim fiziološkim grupama nisu od
primarnog značaja pri proceni pogodnosti vode za upotrebu, populacija "normalno" ili
"prirodno" prisutnih bakterija utiče na organoleptička svojstva, pH, redoks potencijal,
količinu kiseonika i druge karakteristike vode.1,2 U radu su prikazani rezultati
kvalitativne i kvantitativne zastupljenosti gvožđevitih, sulfat redukujućih, nitrifikujućih,
denitrifikujućih, fluorescirajućih i sluz produkujućih bakterija u vodi poreklom iz tri
bunara primenom BART testova (Biological Activity Reaction Tests). Takođe, u radu je
prikazan i broj ukupnih i fekalnih koliformnih bakterija određen metodom IDEXX
Colilert-18/Quanty-Tray 2000 i fekalnih enterokoka metodom Enterolert-E/Quanty-Tray
2000. Dobijeni rezultati ukazuju da postoji pozitivna korelacija između biodiverziteta
različitih grupa testiranih bakterija, što ukazuje na složene ekološke abiotičke i biotičke
odnose u podzemenim vodama i bunarima. Iako je autohtona bakterijska zajednica
uslovljena geološkim svojstvima vode, ove bakterije mogu uticati i limitirati upotrebu
podzemne vode u vodosnabdevanju stupanjem u odnose sa patogenim ili potencijalno
patogenim organizmima
C3  - knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo
T1  - Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6844
ER  - 

@conference{
author = "Vujović, Bojana and Jovičić-Petrović, Jelena and Rašković, Nikola and Vukmanović, Tamara and Kljujev, Igor and Raičević, Vera",
year = "2018",
abstract = "Podzemne vode predstavljaju najočuvaniji deo hidrosfere, zaštićen od globalne
degradacije kvaliteta vode, zbog čega je široko rasprostranjen i često korišćen resurs
vodosnabdevanja. Iako se podzemne vode smatraju mikrobiološki slabo naseljenim
delom biosfere, transformacija organskog ugljenika, transformacija azota, gvožđa i
sumpora između oksidovanog i redukovanog stanja, produkcija metana i drugi
fundamentalni procesi se i u podzemnim vodama odigravaju mikrobiološkom
aktivnošću.1 Iako bakterije koje pripadaju različitim fiziološkim grupama nisu od
primarnog značaja pri proceni pogodnosti vode za upotrebu, populacija "normalno" ili
"prirodno" prisutnih bakterija utiče na organoleptička svojstva, pH, redoks potencijal,
količinu kiseonika i druge karakteristike vode.1,2 U radu su prikazani rezultati
kvalitativne i kvantitativne zastupljenosti gvožđevitih, sulfat redukujućih, nitrifikujućih,
denitrifikujućih, fluorescirajućih i sluz produkujućih bakterija u vodi poreklom iz tri
bunara primenom BART testova (Biological Activity Reaction Tests). Takođe, u radu je
prikazan i broj ukupnih i fekalnih koliformnih bakterija određen metodom IDEXX
Colilert-18/Quanty-Tray 2000 i fekalnih enterokoka metodom Enterolert-E/Quanty-Tray
2000. Dobijeni rezultati ukazuju da postoji pozitivna korelacija između biodiverziteta
različitih grupa testiranih bakterija, što ukazuje na složene ekološke abiotičke i biotičke
odnose u podzemenim vodama i bunarima. Iako je autohtona bakterijska zajednica
uslovljena geološkim svojstvima vode, ove bakterije mogu uticati i limitirati upotrebu
podzemne vode u vodosnabdevanju stupanjem u odnose sa patogenim ili potencijalno
patogenim organizmima",
journal = "knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo",
title = "Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6844"
}

Vujović, B., Jovičić-Petrović, J., Rašković, N., Vukmanović, T., Kljujev, I.,& Raičević, V.. (2018). Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi. in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo.
https://hdl.handle.net/21.15107/rcub_agrospace_6844

Vujović B, Jovičić-Petrović J, Rašković N, Vukmanović T, Kljujev I, Raičević V. Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi. in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo. 2018;.
https://hdl.handle.net/21.15107/rcub_agrospace_6844 .

Vujović, Bojana, Jovičić-Petrović, Jelena, Rašković, Nikola, Vukmanović, Tamara, Kljujev, Igor, Raičević, Vera, "Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi" in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo (2018),
https://hdl.handle.net/21.15107/rcub_agrospace_6844 .

TY  - JOUR
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Vujović, Bojana
AU  - Mirković, Milica
AU  - Rothballer, Michael
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4692
AB  - The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Listeria monocytogenes - Danger for health safety vegetable production
EP  - 31
SP  - 23
VL  - 120
DO  - 10.1016/j.micpath.2018.04.034
ER  - 

@article{
author = "Kljujev, Igor and Raičević, Vera and Jovičić-Petrović, Jelena and Vujović, Bojana and Mirković, Milica and Rothballer, Michael",
year = "2018",
abstract = "The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Listeria monocytogenes - Danger for health safety vegetable production",
pages = "31-23",
volume = "120",
doi = "10.1016/j.micpath.2018.04.034"
}

Kljujev, I., Raičević, V., Jovičić-Petrović, J., Vujović, B., Mirković, M.,& Rothballer, M.. (2018). Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 120, 23-31.
https://doi.org/10.1016/j.micpath.2018.04.034

Kljujev I, Raičević V, Jovičić-Petrović J, Vujović B, Mirković M, Rothballer M. Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis. 2018;120:23-31.
doi:10.1016/j.micpath.2018.04.034 .

Kljujev, Igor, Raičević, Vera, Jovičić-Petrović, Jelena, Vujović, Bojana, Mirković, Milica, Rothballer, Michael, "Listeria monocytogenes - Danger for health safety vegetable production" in Microbial Pathogenesis, 120 (2018):23-31,
https://doi.org/10.1016/j.micpath.2018.04.034 . .

TY  - JOUR
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Vujović, Bojana
AU  - Rothballer, Michael
AU  - Schmid, Michael
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4720
AB  - Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production
EP  - 207
SP  - 199
VL  - 115
DO  - 10.1016/j.micpath.2017.12.020
ER  - 

@article{
author = "Kljujev, Igor and Raičević, Vera and Vujović, Bojana and Rothballer, Michael and Schmid, Michael",
year = "2018",
abstract = "Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production",
pages = "207-199",
volume = "115",
doi = "10.1016/j.micpath.2017.12.020"
}

Kljujev, I., Raičević, V., Vujović, B., Rothballer, M.,& Schmid, M.. (2018). Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 115, 199-207.
https://doi.org/10.1016/j.micpath.2017.12.020

Kljujev I, Raičević V, Vujović B, Rothballer M, Schmid M. Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis. 2018;115:199-207.
doi:10.1016/j.micpath.2017.12.020 .

Kljujev, Igor, Raičević, Vera, Vujović, Bojana, Rothballer, Michael, Schmid, Michael, "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production" in Microbial Pathogenesis, 115 (2018):199-207,
https://doi.org/10.1016/j.micpath.2017.12.020 . .

TY  - JOUR
AU  - Vujović, Bojana
AU  - Rudić, Željka
AU  - Kljujev, Igor
AU  - Rajković, Dobrica
AU  - Božić, Mile
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4228
AB  - Biofilm formation is one of the main characteristic of Pseudomonas aeruginosa strains. This ability provide bacteria to survive in different, usually restrictivesss environmental conditions. In the same time, biofilm forming is a risk in a many field of industry, mainly in food industry. It is known that diverse P. aeruginosa strains from various environmental sources have different potention to form biofilms. In this paper it is examinated the potention to form biofilm four isolates of P. aeruginosa and based of this results, tested isolates have classificated into four groups. Biofilm formation tested in microtitar plates during the 24 hours at changed conditions of temperature (37°C and 22°C), pH (6 and 7) and concentration of NaCl (1% and 2%). Obtained results have shown that isolated P. aeruginosa have unsimilar ability to form biofilms. Biomass of formed biofilm was greater at 22°C than at 37°C. Also, acid conditions and higher concentration of salt had no inhibitory effect to biofilm forming process.
AB  - Stvaranje biofilmova predstavlja jednu od glavnih osobina Pseudomonas aeruginosa koja omogućava ovoj bakteriji preživljavanje u različitim, često nepovoljnim uslovima životne sredine. Međutim, formiranje biofilmova predstavlja i faktor rizika u mnogim oblastima industrije, posebno prehrambene, pošto prisustvo čvrstih površina pruža dobre uslove za formiranje biofilma. Kako je poznato da izolati P. aeruginosa koji potiču iz različitih uzoraka poreklom iz životne sredine imaju različit potencijal da formiraju biofilmove, u ovom radu je ispitana sposobnost formiranja biofilmova četiri izolata ove bakterije i izvršena njihova klasifikacija u odnosu na ovu osobinu. Primenjen je statički test u mikrotitar pločama u trajanju od 24 sata u različitim uslovima temperature (37°C i 22°C), pH (6 i 7) i koncentracije NaCl (1 i 2%). Dobijeni rezultati su pokazali da izolati P. aeruginosa imaju različitu sposobnost da formiraju biofilmove, da je masa formiranih biofilmova bila veća na 22°C, kao i da promenjeni uslovi sredine nisu inhibirali stvaranje biofilmova.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Potential of pseudomonas aeruginosa environmental isolates for biofilm formation
T1  - Potencijal formiranja biofilmova pseudomonas aeruginosa iz životne sredine
EP  - 454
IS  - 3
SP  - 449
VL  - 57
DO  - 10.5937/ZasMat1603449V
ER  - 

@article{
author = "Vujović, Bojana and Rudić, Željka and Kljujev, Igor and Rajković, Dobrica and Božić, Mile and Raičević, Vera",
year = "2016",
abstract = "Biofilm formation is one of the main characteristic of Pseudomonas aeruginosa strains. This ability provide bacteria to survive in different, usually restrictivesss environmental conditions. In the same time, biofilm forming is a risk in a many field of industry, mainly in food industry. It is known that diverse P. aeruginosa strains from various environmental sources have different potention to form biofilms. In this paper it is examinated the potention to form biofilm four isolates of P. aeruginosa and based of this results, tested isolates have classificated into four groups. Biofilm formation tested in microtitar plates during the 24 hours at changed conditions of temperature (37°C and 22°C), pH (6 and 7) and concentration of NaCl (1% and 2%). Obtained results have shown that isolated P. aeruginosa have unsimilar ability to form biofilms. Biomass of formed biofilm was greater at 22°C than at 37°C. Also, acid conditions and higher concentration of salt had no inhibitory effect to biofilm forming process., Stvaranje biofilmova predstavlja jednu od glavnih osobina Pseudomonas aeruginosa koja omogućava ovoj bakteriji preživljavanje u različitim, često nepovoljnim uslovima životne sredine. Međutim, formiranje biofilmova predstavlja i faktor rizika u mnogim oblastima industrije, posebno prehrambene, pošto prisustvo čvrstih površina pruža dobre uslove za formiranje biofilma. Kako je poznato da izolati P. aeruginosa koji potiču iz različitih uzoraka poreklom iz životne sredine imaju različit potencijal da formiraju biofilmove, u ovom radu je ispitana sposobnost formiranja biofilmova četiri izolata ove bakterije i izvršena njihova klasifikacija u odnosu na ovu osobinu. Primenjen je statički test u mikrotitar pločama u trajanju od 24 sata u različitim uslovima temperature (37°C i 22°C), pH (6 i 7) i koncentracije NaCl (1 i 2%). Dobijeni rezultati su pokazali da izolati P. aeruginosa imaju različitu sposobnost da formiraju biofilmove, da je masa formiranih biofilmova bila veća na 22°C, kao i da promenjeni uslovi sredine nisu inhibirali stvaranje biofilmova.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Potential of pseudomonas aeruginosa environmental isolates for biofilm formation, Potencijal formiranja biofilmova pseudomonas aeruginosa iz životne sredine",
pages = "454-449",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603449V"
}

Vujović, B., Rudić, Ž., Kljujev, I., Rajković, D., Božić, M.,& Raičević, V.. (2016). Potential of pseudomonas aeruginosa environmental isolates for biofilm formation. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 449-454.
https://doi.org/10.5937/ZasMat1603449V

Vujović B, Rudić Ž, Kljujev I, Rajković D, Božić M, Raičević V. Potential of pseudomonas aeruginosa environmental isolates for biofilm formation. in Zaštita materijala. 2016;57(3):449-454.
doi:10.5937/ZasMat1603449V .

Vujović, Bojana, Rudić, Željka, Kljujev, Igor, Rajković, Dobrica, Božić, Mile, Raičević, Vera, "Potential of pseudomonas aeruginosa environmental isolates for biofilm formation" in Zaštita materijala, 57, no. 3 (2016):449-454,
https://doi.org/10.5937/ZasMat1603449V . .

TY  - THES
AU  - Kljujev, Igor
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/16
AB  - Consumption of fresh vegetables and fruits is a healthy diet supply and it is recommended as prevention of illnesses. There is an increasing trend in consumption of fresh vegetables and fruits in the world, and their microbiological quality is very important for public human health and protection. Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp., Escherichia coli, E. coli O157:H7, L. monocytogenes on the fresh vegetables. Salmonellosis is connected with tomato, sprouts, cantaloupe etc. E. coli and E. coli O157:H infections are linked with lettuce, sprouts, carrot etc. There has been an increasing number of outbreaks caused by human pathogen on fresh vegetables and fruits in the recent years. Contaminated irrigation water is a very effective vector of the transmission of human pathogen to plants, so microbiological safe water has special importance in health safety food production. Contamination of fresh vegetables by human pathogen bacteria depends on bacterial ability to colonize plant. In the case of microbiological contamination of fresh vegetables and fruits, it is very difficult to carry out decontamination of product, because it is impossible to remove all attached bacteria only by washing. The goal of this research is an investigation of presence and identification of pathogen bacteria in the irrigation water and on vegetables, transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the human pathogen to colonize the surface and endophyte of root, stem and leaf of different vegetable species. Microbiological quality of water and vegetables is estimated on the basis of the presence and number of coliform bacteria, as well as certain species of pathogen bacteria. Isolated strains of human pathogen bacteria are identified by their morphological, ecological and biochemical properties. Transmission of pathogen bacteria from irrigation water to plants, as well as colonization of plants by human pathogen bacteria was investigated by: applying PCR method, using green fluorescent protein transformed bacteria, fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM)...
AB  - Konzumiranje svežeg povrća i voća je satavni deo zdrave ishrane i preporučuje se kao prevencija nastanka raznih obolenja. U svetu postoji trend povećanja konzumiranja svežeg povrća i voća, pa je njegov mikrobiološki kvalitet od izuzetne vaznosti za javno zdravlje i bezbednost. Kontaminacija povrća i voća je rezultat prisustva patogenih bakterija, koje mogu kontaminirati proizvode u bilo kom delu proizvodnog lanca. Na svežem povrću je moguće prisustvo: Salmonella spp., Escherichia coli, E. coli O157:H7, L. monocytogenes. Salmoneloze se povezuju sa paradajizom, klicama raznih biljaka, dinjom. Infekcije izazvane E. coli i E. coli O157:H7 su povezane sa zelenom salatom, klicama raznih biljaka, mrkvom i dr. Poslednjih godina je u porastu broj epidemija izazvanih patogenima na svežem povrću i voću. Kontaminirana voda za navodnjavanje je efikasan vektor prenosa patogena na biljke, pa mikrobiološki ispravna voda ima poseban značaj u proizvodnji zdrastveno bezbedne hrane. Kontamincija svežeg povrća patogenim bakterijama zavisi od sposobnosti ovih bakterija da kolonizuju biljku. U slučaju pojave mikrobiološke kontaminacije svežeg povrća i voća, teško je izvršiti dekontaminaciju proizvoda, jer je bakterije nemoguće potpuno ukloniti samo pranjem vodom. Cilj ovog rada je ispitivanje prisustva i identifikacija patogenih bakterija u vodi za navodnjavanje i na povrću, proučavanje prenosa patogenih bakterija iz vode za navodnjavanje do biljke i praćenje sposobosti patogenih bakterija da površinski i endofitno kolonizuju koren, stablo i list različitih vrsta povrća. Mikrobiološki kvalitet vode i povrća je procenjivan na osnovu prisustva i broja koliformnih bakterija, kao i brojnosti određenih vrsta patogenih bakterija. Izolovani sojevi patogenih bakterija su identifikovani na osnovu njihovih morfoloških, ekoloških i biohemijskih osobina. Prenos patogenih bakterija iz vode za navodnjavanje do biljke, kao i kolonizacija biljaka patogenim bakterijama je proučavana primenom PCR metode, green fluorescent protein genetske transformacije bakterija, metodom fluorescence in situ hybridization (FISH) i laser skening konfokalnom mikroskopijom (CLSM)...
PB  - Univerzitet u Beogradu, Poljoprivredni fakultet
T1  - Contamination of plants by pathogenic bacteria from irrigation water
T1  - Kontaminacija biljaka patogenim bakterijama iz vode za navodnjavanje
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2549
ER  - 

@phdthesis{
author = "Kljujev, Igor",
year = "2013",
abstract = "Consumption of fresh vegetables and fruits is a healthy diet supply and it is recommended as prevention of illnesses. There is an increasing trend in consumption of fresh vegetables and fruits in the world, and their microbiological quality is very important for public human health and protection. Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp., Escherichia coli, E. coli O157:H7, L. monocytogenes on the fresh vegetables. Salmonellosis is connected with tomato, sprouts, cantaloupe etc. E. coli and E. coli O157:H infections are linked with lettuce, sprouts, carrot etc. There has been an increasing number of outbreaks caused by human pathogen on fresh vegetables and fruits in the recent years. Contaminated irrigation water is a very effective vector of the transmission of human pathogen to plants, so microbiological safe water has special importance in health safety food production. Contamination of fresh vegetables by human pathogen bacteria depends on bacterial ability to colonize plant. In the case of microbiological contamination of fresh vegetables and fruits, it is very difficult to carry out decontamination of product, because it is impossible to remove all attached bacteria only by washing. The goal of this research is an investigation of presence and identification of pathogen bacteria in the irrigation water and on vegetables, transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the human pathogen to colonize the surface and endophyte of root, stem and leaf of different vegetable species. Microbiological quality of water and vegetables is estimated on the basis of the presence and number of coliform bacteria, as well as certain species of pathogen bacteria. Isolated strains of human pathogen bacteria are identified by their morphological, ecological and biochemical properties. Transmission of pathogen bacteria from irrigation water to plants, as well as colonization of plants by human pathogen bacteria was investigated by: applying PCR method, using green fluorescent protein transformed bacteria, fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM)..., Konzumiranje svežeg povrća i voća je satavni deo zdrave ishrane i preporučuje se kao prevencija nastanka raznih obolenja. U svetu postoji trend povećanja konzumiranja svežeg povrća i voća, pa je njegov mikrobiološki kvalitet od izuzetne vaznosti za javno zdravlje i bezbednost. Kontaminacija povrća i voća je rezultat prisustva patogenih bakterija, koje mogu kontaminirati proizvode u bilo kom delu proizvodnog lanca. Na svežem povrću je moguće prisustvo: Salmonella spp., Escherichia coli, E. coli O157:H7, L. monocytogenes. Salmoneloze se povezuju sa paradajizom, klicama raznih biljaka, dinjom. Infekcije izazvane E. coli i E. coli O157:H7 su povezane sa zelenom salatom, klicama raznih biljaka, mrkvom i dr. Poslednjih godina je u porastu broj epidemija izazvanih patogenima na svežem povrću i voću. Kontaminirana voda za navodnjavanje je efikasan vektor prenosa patogena na biljke, pa mikrobiološki ispravna voda ima poseban značaj u proizvodnji zdrastveno bezbedne hrane. Kontamincija svežeg povrća patogenim bakterijama zavisi od sposobnosti ovih bakterija da kolonizuju biljku. U slučaju pojave mikrobiološke kontaminacije svežeg povrća i voća, teško je izvršiti dekontaminaciju proizvoda, jer je bakterije nemoguće potpuno ukloniti samo pranjem vodom. Cilj ovog rada je ispitivanje prisustva i identifikacija patogenih bakterija u vodi za navodnjavanje i na povrću, proučavanje prenosa patogenih bakterija iz vode za navodnjavanje do biljke i praćenje sposobosti patogenih bakterija da površinski i endofitno kolonizuju koren, stablo i list različitih vrsta povrća. Mikrobiološki kvalitet vode i povrća je procenjivan na osnovu prisustva i broja koliformnih bakterija, kao i brojnosti određenih vrsta patogenih bakterija. Izolovani sojevi patogenih bakterija su identifikovani na osnovu njihovih morfoloških, ekoloških i biohemijskih osobina. Prenos patogenih bakterija iz vode za navodnjavanje do biljke, kao i kolonizacija biljaka patogenim bakterijama je proučavana primenom PCR metode, green fluorescent protein genetske transformacije bakterija, metodom fluorescence in situ hybridization (FISH) i laser skening konfokalnom mikroskopijom (CLSM)...",
publisher = "Univerzitet u Beogradu, Poljoprivredni fakultet",
title = "Contamination of plants by pathogenic bacteria from irrigation water, Kontaminacija biljaka patogenim bakterijama iz vode za navodnjavanje",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2549"
}

Kljujev, I.. (2013). Contamination of plants by pathogenic bacteria from irrigation water. 
Univerzitet u Beogradu, Poljoprivredni fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_2549

Kljujev I. Contamination of plants by pathogenic bacteria from irrigation water. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_2549 .

Kljujev, Igor, "Contamination of plants by pathogenic bacteria from irrigation water" (2013),
https://hdl.handle.net/21.15107/rcub_nardus_2549 .

TY  - JOUR
AU  - Lalević, Blažo
AU  - Jović, Jelena
AU  - Raičević, Vera
AU  - Kljujev, Igor
AU  - Kiković, Dragan
AU  - Hamidović, Saud
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2922
AB  - Methyl-tert-butyl ether (MTBE) has been used to replace the toxic compounds from gasoline and to reduce emission of air pollutants. Due to its intensive use, MTBE has become one of the most important environment pollutants. The aim of this study was the isolation and identification of the bacteria from wastewater sample of 'HIP Petrohemija', Pančevo (Serbia), capable of MTBE biodegradation. The results of the investigation showed that only the bacterial isolate 27/1 was capable of growth on MTBE. The result of sequence analyzes of 16S rDNA showed that this bacterial isolate belongs to the Kocuria sp. After the incubation period of 86 days, the degradation rates of initial MTBE concentration of 25 and 125 μg/ml were 55 and 36%, respectively. These results indicated that bacteria Kocuria sp. is successfully adapted on MTBE and can be potentially used in bioremediation of soils and waters contaminated with MTBE.
AB  - Metil tercijarni butil etar (MTBE) uveden je u proizvodnju s ciljem zamene toksičnih komponenti iz benzina, povećanja oktanskog broja goriva i redukcije aerozagađenja. Usled intenzivne primene, perzistentnosti i mobilnosti, MTBE je posle svega nekoliko godina postao značajan polutant u životnoj sredini. Cilj ovog rada je izolacija i identifikacija bakterija iz uzorka otpadne vode poreklom iz 'HIP Petrohemije', Pančevo, sposobnih da vrše degradaciju MTBE-a. Od 11 bakterijskih izolata, svrstanih u tri grupe po morfološkim osobinama, samo je jedan izolat, označen kao 27/1, pokazao sposobnost rasta na MTBE-u kao jedinstvenom izvoru ugljenika i energije i odabran je za dalja istraživanja. Ovaj izolat ima sitne crvenkaste kolonije na podlozi 0,1×TSA. Ćelije su okruglastog oblika, ne stvaraju spore a po Gramu se boje pozitivno. Primenom API i APIWEB sistema, ovaj izolat pokazuje najveći stepen sličnosti sa bakterijskom vrstom Kocuria rosea. Sekvenca izolata 27/1 dobijena sekvencionom 16S rDNA analizom se sastoji od 1407 nukleotida, na osnovu čega je ovaj izolat identifikovan kao Kocuria sp. Nakon inkubacije od 86 dana, stepen degradacije početnih koncentracija MTBE-a od 25 i 125 μg/ml iznosio je 55, odnosno 36%. Pri početnoj koncentraciji od 25 μg/ml optička gustina i brojnost bakterija se smanjuju u prvih 15 dana inkubacije, dok je pri početnoj koncentraciji od 125 μg/ml zabeleženo smanjenje optičke gustine i broja bakterija u prvih 30 dana. Nakon ovog perioda, stepen bakterijskog rasta se povećava. U početnim fazama Inkubacije, stepen degradacije MTBE-a nije visok, dok je krajem inkubacionog perioda konstatovan najveći stepen degradacije. Ovi rezultati ukazuju na uspešnu adaptaciju bakterije Kocuria sp. na prisustvo MTBE-a, što opravdava njenu primenu u bioremedijaciji zemljišta i voda kontaminiranih MTBE-om.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Biodegradation of methyl-tert-butyl ether by Kocuria sp.
T1  - Biodegradacija metil tercijarnog butil etra pomoću Kocuria sp.
EP  - 722
IS  - 5
SP  - 717
VL  - 66
DO  - 10.2298/HEMIND120110019L
ER  - 

@article{
author = "Lalević, Blažo and Jović, Jelena and Raičević, Vera and Kljujev, Igor and Kiković, Dragan and Hamidović, Saud",
year = "2012",
abstract = "Methyl-tert-butyl ether (MTBE) has been used to replace the toxic compounds from gasoline and to reduce emission of air pollutants. Due to its intensive use, MTBE has become one of the most important environment pollutants. The aim of this study was the isolation and identification of the bacteria from wastewater sample of 'HIP Petrohemija', Pančevo (Serbia), capable of MTBE biodegradation. The results of the investigation showed that only the bacterial isolate 27/1 was capable of growth on MTBE. The result of sequence analyzes of 16S rDNA showed that this bacterial isolate belongs to the Kocuria sp. After the incubation period of 86 days, the degradation rates of initial MTBE concentration of 25 and 125 μg/ml were 55 and 36%, respectively. These results indicated that bacteria Kocuria sp. is successfully adapted on MTBE and can be potentially used in bioremediation of soils and waters contaminated with MTBE., Metil tercijarni butil etar (MTBE) uveden je u proizvodnju s ciljem zamene toksičnih komponenti iz benzina, povećanja oktanskog broja goriva i redukcije aerozagađenja. Usled intenzivne primene, perzistentnosti i mobilnosti, MTBE je posle svega nekoliko godina postao značajan polutant u životnoj sredini. Cilj ovog rada je izolacija i identifikacija bakterija iz uzorka otpadne vode poreklom iz 'HIP Petrohemije', Pančevo, sposobnih da vrše degradaciju MTBE-a. Od 11 bakterijskih izolata, svrstanih u tri grupe po morfološkim osobinama, samo je jedan izolat, označen kao 27/1, pokazao sposobnost rasta na MTBE-u kao jedinstvenom izvoru ugljenika i energije i odabran je za dalja istraživanja. Ovaj izolat ima sitne crvenkaste kolonije na podlozi 0,1×TSA. Ćelije su okruglastog oblika, ne stvaraju spore a po Gramu se boje pozitivno. Primenom API i APIWEB sistema, ovaj izolat pokazuje najveći stepen sličnosti sa bakterijskom vrstom Kocuria rosea. Sekvenca izolata 27/1 dobijena sekvencionom 16S rDNA analizom se sastoji od 1407 nukleotida, na osnovu čega je ovaj izolat identifikovan kao Kocuria sp. Nakon inkubacije od 86 dana, stepen degradacije početnih koncentracija MTBE-a od 25 i 125 μg/ml iznosio je 55, odnosno 36%. Pri početnoj koncentraciji od 25 μg/ml optička gustina i brojnost bakterija se smanjuju u prvih 15 dana inkubacije, dok je pri početnoj koncentraciji od 125 μg/ml zabeleženo smanjenje optičke gustine i broja bakterija u prvih 30 dana. Nakon ovog perioda, stepen bakterijskog rasta se povećava. U početnim fazama Inkubacije, stepen degradacije MTBE-a nije visok, dok je krajem inkubacionog perioda konstatovan najveći stepen degradacije. Ovi rezultati ukazuju na uspešnu adaptaciju bakterije Kocuria sp. na prisustvo MTBE-a, što opravdava njenu primenu u bioremedijaciji zemljišta i voda kontaminiranih MTBE-om.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Biodegradation of methyl-tert-butyl ether by Kocuria sp., Biodegradacija metil tercijarnog butil etra pomoću Kocuria sp.",
pages = "722-717",
number = "5",
volume = "66",
doi = "10.2298/HEMIND120110019L"
}

Lalević, B., Jović, J., Raičević, V., Kljujev, I., Kiković, D.,& Hamidović, S.. (2012). Biodegradation of methyl-tert-butyl ether by Kocuria sp.. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 66(5), 717-722.
https://doi.org/10.2298/HEMIND120110019L

Lalević B, Jović J, Raičević V, Kljujev I, Kiković D, Hamidović S. Biodegradation of methyl-tert-butyl ether by Kocuria sp.. in Hemijska industrija. 2012;66(5):717-722.
doi:10.2298/HEMIND120110019L .

Lalević, Blažo, Jović, Jelena, Raičević, Vera, Kljujev, Igor, Kiković, Dragan, Hamidović, Saud, "Biodegradation of methyl-tert-butyl ether by Kocuria sp." in Hemijska industrija, 66, no. 5 (2012):717-722,
https://doi.org/10.2298/HEMIND120110019L . .

TY  - JOUR
AU  - Petković, Sava
AU  - Gregorić, Eniko
AU  - Slepcević, Vesna
AU  - Blagojević, Srdjan
AU  - Gajić, Boško
AU  - Kljujev, Igor
AU  - Žarković, Branka
AU  - Djurović, Nevenka
AU  - Drasković, Radovan
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2727
AB  - In suburban Belgrade, there are some 200 local water supply systems which are not connected to either the Belgrade Water Supply System or to supply systems operated by municipal utilities. The small systems in Belgrade suburbs are either operated by local municipality (local government) or even by the group of local citizens who have neither technical capability nor financial resources to do it properly. Roughly 200,000 of Belgrade's inhabitants obtain their drinking water from these water supply systems. The water quality delivered by these local water supply systems is often compromised in terms of microbiological, physical and/or chemical compliance with drinking water standards in addition to the general lack of strategy on water safety plans and risk assessment. WHO Guidelines on water quality standards as well as the recommendations on safety plans and whole risk assessment are strictly respected in the main (central) Water Supply System in Belgrade. Most frequently, elevated concentrations of ammonia, nitrites, nitrates and iron lead to lack of chemical compliance, while elevated counts of aerobic mesophilic bacteria and the presence of bacteria indicative of faecal pollution tend to be behind microbiological lack of compliance with drinking water standards. In most cases, failure to meet drinking water standards can be attributed to groundwater pollution. No sewer system exists in these areas, and wastewater from septic tanks, in practice infiltration wells, is in direct contact with groundwater. Of a total of 72 laboratory-tested drinking water samples, 51.3% failed to meet physical and/or chemical standards, and 73.6% failed to meet microbiological standards. Groundwater pollution can only be prevented if wastewater disposal system is provided for all households and all suburban residential areas which obtain their water supply from local water supply systems. Some possible mitigation measures have been indicated. In the interim period, water must be disinfected continually, and the feasibility of ozonation or UV irradiation, in addition to chlorination, should be assessed.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Urban Water Journal
T1  - Contamination of local water supply systems in suburban Belgrade
EP  - 92
IS  - 2
SP  - 79
VL  - 8
DO  - 10.1080/1573062X.2010.546862
ER  - 

@article{
author = "Petković, Sava and Gregorić, Eniko and Slepcević, Vesna and Blagojević, Srdjan and Gajić, Boško and Kljujev, Igor and Žarković, Branka and Djurović, Nevenka and Drasković, Radovan",
year = "2011",
abstract = "In suburban Belgrade, there are some 200 local water supply systems which are not connected to either the Belgrade Water Supply System or to supply systems operated by municipal utilities. The small systems in Belgrade suburbs are either operated by local municipality (local government) or even by the group of local citizens who have neither technical capability nor financial resources to do it properly. Roughly 200,000 of Belgrade's inhabitants obtain their drinking water from these water supply systems. The water quality delivered by these local water supply systems is often compromised in terms of microbiological, physical and/or chemical compliance with drinking water standards in addition to the general lack of strategy on water safety plans and risk assessment. WHO Guidelines on water quality standards as well as the recommendations on safety plans and whole risk assessment are strictly respected in the main (central) Water Supply System in Belgrade. Most frequently, elevated concentrations of ammonia, nitrites, nitrates and iron lead to lack of chemical compliance, while elevated counts of aerobic mesophilic bacteria and the presence of bacteria indicative of faecal pollution tend to be behind microbiological lack of compliance with drinking water standards. In most cases, failure to meet drinking water standards can be attributed to groundwater pollution. No sewer system exists in these areas, and wastewater from septic tanks, in practice infiltration wells, is in direct contact with groundwater. Of a total of 72 laboratory-tested drinking water samples, 51.3% failed to meet physical and/or chemical standards, and 73.6% failed to meet microbiological standards. Groundwater pollution can only be prevented if wastewater disposal system is provided for all households and all suburban residential areas which obtain their water supply from local water supply systems. Some possible mitigation measures have been indicated. In the interim period, water must be disinfected continually, and the feasibility of ozonation or UV irradiation, in addition to chlorination, should be assessed.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Urban Water Journal",
title = "Contamination of local water supply systems in suburban Belgrade",
pages = "92-79",
number = "2",
volume = "8",
doi = "10.1080/1573062X.2010.546862"
}

Petković, S., Gregorić, E., Slepcević, V., Blagojević, S., Gajić, B., Kljujev, I., Žarković, B., Djurović, N.,& Drasković, R.. (2011). Contamination of local water supply systems in suburban Belgrade. in Urban Water Journal
Taylor & Francis Ltd, Abingdon., 8(2), 79-92.
https://doi.org/10.1080/1573062X.2010.546862

Petković S, Gregorić E, Slepcević V, Blagojević S, Gajić B, Kljujev I, Žarković B, Djurović N, Drasković R. Contamination of local water supply systems in suburban Belgrade. in Urban Water Journal. 2011;8(2):79-92.
doi:10.1080/1573062X.2010.546862 .

Petković, Sava, Gregorić, Eniko, Slepcević, Vesna, Blagojević, Srdjan, Gajić, Boško, Kljujev, Igor, Žarković, Branka, Djurović, Nevenka, Drasković, Radovan, "Contamination of local water supply systems in suburban Belgrade" in Urban Water Journal, 8, no. 2 (2011):79-92,
https://doi.org/10.1080/1573062X.2010.546862 . .

TY  - JOUR
AU  - Forslund, A.
AU  - Ensink, J.H.J.
AU  - Battilani, A.
AU  - Kljujev, Igor
AU  - Gola, S.
AU  - Raičević, Vera
AU  - Jovanović, Zorica
AU  - Stikić, Radmila
AU  - Sandei, L.
AU  - Fletcher, T.
AU  - Dalsgaard, A.
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2346
AB  - Clean water has become one of the main limiting factors in agricultural food production in Europe, especially for countries around the Mediterranean, who now face more severe and-frequent seasonal water shortages. In order to overcome water shortages the European Water Framework Directive encourages and promotes the use of treated urban wastewater in agriculture. However, the use of poor quality water in agriculture poses potential health risks. The application of wastewater through subsurface drip irrigation lines could possibly overcome public health concerns by minimizing contact with wastewater by farmers, farm workers but it is uncertain if the risk for consumers of wastewater irrigated produces would be acceptable. The objective of the current study was therefore to assess whether subsurface irrigation of potatoes with low quality water was associated with higher food safety and reduced human health risks as compared with surface irrigation. The microbial quality of soil and potatoes irrigated by sprinkler, furrow and subsurface drip irrigation, using treated urban wastewater, canal water and tap water were compared at experimental sites near Belgrade, Serbia and in Bologna, Italy. Water, soil and potato samples were collected from March 2007 to September 2008 and their faecal contamination estimated by enumeration of the faecal indicator Escherichia coli. In addition, water and potatoes in Italy were analysed for the presence of helminth eggs, another important indicator of faecal pollution. A quantitative microbial risk assessment (QMRA) model combined with Monte Carlo simulations was used to assess whether the different irrigation practices and associated health risks complied with guidelines set by the World Health Organization (WHO). The study found low levels of E. coli in irrigation water (Italy mean value: 1.7 colony forming units (cfu)/ml and Serbia 11 cfu/ml), as well as in soil (Italy mean: 1.0 cfu/g and Serbia 1.1 cfu/g). Similar low concentrations of E. call were found on potatoes (Italy mean: 1.0 cfu/g and Serbia 0.0 cfu/g). The vast majority (442/516) of the collected different samples were free of E. coli. No helminth eggs were found in any types of irrigation water or on the surface of potatoes. The risk assessment models found the use of treated wastewater to exceed the levels of risks for gastro-intestinal disease (1.0 x 10(-3) disease risk) as recommended by the World Health Organization (WHO) for the accidental ingestion of soil by farmers (Serbia: 0.22 and Italy: 5.7 x 10(-2)). However, samples that exceeded disease risks set by the WHO were collected before initiation of wastewater irrigation and were limited to a few numbers of samples, which would indicate environmental contamination not linked to irrigation practice. Disease risk from consumption of potatoes in Italy and in Serbia was found to be within acceptable levels. No relationship was found between E. coli concentrations in irrigation water, soil and produce. Similar lack of association was found for E. coli findings in sprinkler, furrow or subsurface drip irrigated soils and produce. This indicates that subsurface drip irrigation can be practiced while ensuring food safety and protecting the health of consumers and farmers.
PB  - Elsevier, Amsterdam
T2  - Agricultural Water Management
T1  - Faecal contamination and hygiene aspect associated with the use of treated wastewater and canal water for irrigation of potatoes (Solanum tuberosum)
EP  - 450
IS  - 3
SP  - 440
VL  - 98
DO  - 10.1016/j.agwat.2010.10.007
ER  - 

@article{
author = "Forslund, A. and Ensink, J.H.J. and Battilani, A. and Kljujev, Igor and Gola, S. and Raičević, Vera and Jovanović, Zorica and Stikić, Radmila and Sandei, L. and Fletcher, T. and Dalsgaard, A.",
year = "2010",
abstract = "Clean water has become one of the main limiting factors in agricultural food production in Europe, especially for countries around the Mediterranean, who now face more severe and-frequent seasonal water shortages. In order to overcome water shortages the European Water Framework Directive encourages and promotes the use of treated urban wastewater in agriculture. However, the use of poor quality water in agriculture poses potential health risks. The application of wastewater through subsurface drip irrigation lines could possibly overcome public health concerns by minimizing contact with wastewater by farmers, farm workers but it is uncertain if the risk for consumers of wastewater irrigated produces would be acceptable. The objective of the current study was therefore to assess whether subsurface irrigation of potatoes with low quality water was associated with higher food safety and reduced human health risks as compared with surface irrigation. The microbial quality of soil and potatoes irrigated by sprinkler, furrow and subsurface drip irrigation, using treated urban wastewater, canal water and tap water were compared at experimental sites near Belgrade, Serbia and in Bologna, Italy. Water, soil and potato samples were collected from March 2007 to September 2008 and their faecal contamination estimated by enumeration of the faecal indicator Escherichia coli. In addition, water and potatoes in Italy were analysed for the presence of helminth eggs, another important indicator of faecal pollution. A quantitative microbial risk assessment (QMRA) model combined with Monte Carlo simulations was used to assess whether the different irrigation practices and associated health risks complied with guidelines set by the World Health Organization (WHO). The study found low levels of E. coli in irrigation water (Italy mean value: 1.7 colony forming units (cfu)/ml and Serbia 11 cfu/ml), as well as in soil (Italy mean: 1.0 cfu/g and Serbia 1.1 cfu/g). Similar low concentrations of E. call were found on potatoes (Italy mean: 1.0 cfu/g and Serbia 0.0 cfu/g). The vast majority (442/516) of the collected different samples were free of E. coli. No helminth eggs were found in any types of irrigation water or on the surface of potatoes. The risk assessment models found the use of treated wastewater to exceed the levels of risks for gastro-intestinal disease (1.0 x 10(-3) disease risk) as recommended by the World Health Organization (WHO) for the accidental ingestion of soil by farmers (Serbia: 0.22 and Italy: 5.7 x 10(-2)). However, samples that exceeded disease risks set by the WHO were collected before initiation of wastewater irrigation and were limited to a few numbers of samples, which would indicate environmental contamination not linked to irrigation practice. Disease risk from consumption of potatoes in Italy and in Serbia was found to be within acceptable levels. No relationship was found between E. coli concentrations in irrigation water, soil and produce. Similar lack of association was found for E. coli findings in sprinkler, furrow or subsurface drip irrigated soils and produce. This indicates that subsurface drip irrigation can be practiced while ensuring food safety and protecting the health of consumers and farmers.",
publisher = "Elsevier, Amsterdam",
journal = "Agricultural Water Management",
title = "Faecal contamination and hygiene aspect associated with the use of treated wastewater and canal water for irrigation of potatoes (Solanum tuberosum)",
pages = "450-440",
number = "3",
volume = "98",
doi = "10.1016/j.agwat.2010.10.007"
}

Forslund, A., Ensink, J.H.J., Battilani, A., Kljujev, I., Gola, S., Raičević, V., Jovanović, Z., Stikić, R., Sandei, L., Fletcher, T.,& Dalsgaard, A.. (2010). Faecal contamination and hygiene aspect associated with the use of treated wastewater and canal water for irrigation of potatoes (Solanum tuberosum). in Agricultural Water Management
Elsevier, Amsterdam., 98(3), 440-450.
https://doi.org/10.1016/j.agwat.2010.10.007

Forslund A, Ensink J, Battilani A, Kljujev I, Gola S, Raičević V, Jovanović Z, Stikić R, Sandei L, Fletcher T, Dalsgaard A. Faecal contamination and hygiene aspect associated with the use of treated wastewater and canal water for irrigation of potatoes (Solanum tuberosum). in Agricultural Water Management. 2010;98(3):440-450.
doi:10.1016/j.agwat.2010.10.007 .

Forslund, A., Ensink, J.H.J., Battilani, A., Kljujev, Igor, Gola, S., Raičević, Vera, Jovanović, Zorica, Stikić, Radmila, Sandei, L., Fletcher, T., Dalsgaard, A., "Faecal contamination and hygiene aspect associated with the use of treated wastewater and canal water for irrigation of potatoes (Solanum tuberosum)" in Agricultural Water Management, 98, no. 3 (2010):440-450,
https://doi.org/10.1016/j.agwat.2010.10.007 . .

TY  - JOUR
AU  - Dulić, Zorka
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Živić, Ivana
AU  - Marković, Zoran
AU  - Stanković, Marko
AU  - Poleksić, Vesna
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1794
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Estimation of irrigation water quality using coliform bacteria, zooplankton and zoobenthos as indicators
EP  - 12P
IS  - 1
SP  - 11P
VL  - 60
DO  - 10.2298/ABS080111PD
ER  - 

@article{
author = "Dulić, Zorka and Kljujev, Igor and Raičević, Vera and Živić, Ivana and Marković, Zoran and Stanković, Marko and Poleksić, Vesna",
year = "2008",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Estimation of irrigation water quality using coliform bacteria, zooplankton and zoobenthos as indicators",
pages = "12P-11P",
number = "1",
volume = "60",
doi = "10.2298/ABS080111PD"
}

Dulić, Z., Kljujev, I., Raičević, V., Živić, I., Marković, Z., Stanković, M.,& Poleksić, V.. (2008). Estimation of irrigation water quality using coliform bacteria, zooplankton and zoobenthos as indicators. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(1), 11P-12P.
https://doi.org/10.2298/ABS080111PD

Dulić Z, Kljujev I, Raičević V, Živić I, Marković Z, Stanković M, Poleksić V. Estimation of irrigation water quality using coliform bacteria, zooplankton and zoobenthos as indicators. in Archives of Biological Sciences. 2008;60(1):11P-12P.
doi:10.2298/ABS080111PD .

Dulić, Zorka, Kljujev, Igor, Raičević, Vera, Živić, Ivana, Marković, Zoran, Stanković, Marko, Poleksić, Vesna, "Estimation of irrigation water quality using coliform bacteria, zooplankton and zoobenthos as indicators" in Archives of Biological Sciences, 60, no. 1 (2008):11P-12P,
https://doi.org/10.2298/ABS080111PD . .

TY  - JOUR
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1645
AB  - Contamination of fruits and vegetables can arise as a result of presence of microorganisms as bacteria, viruses and parasites. Pathogenic bacteria often found on vegetables and fruits are: Salmonella spp., Shigella spp., Escherichia coli, E. coli O157:H7, Campylobacter sp., Listeria monocytogenes, Clostridium botulinum, Bacillus cereus. Microbial contamination depends on different factors including soil conditions, water for irrigation, organic fertilisers, harvesting or post harvest treatment. Several types' of decontamination treatment are known to be partially effective in removing disease-causing organisms from the surface of whole and cut raw fruits and vegetables or from contact surfaces during han­dling. Disinfection methods include chlorine, organic acid, ozone, UV light. Several vegetable and fruit species were tested for decontamination. The crop contamination was done by keeping lettuce, tomato and apple fruit in artificially contaminated water. Decontamination procedure included disinfection by UV and different organic solutions included: vine vinegar, apple vinegar, vinegar and citric acid. The number of E. coli and Salmonella sp. on the surface of vegetable species was determined by using Petri-film method (3M, USA). The biggest effect of decontamination which resulted in the smallest number of bacteria was obtained after 30 min. of lettuce exposure to citric acid. Microbiological results indirectly pointed out on potential risk of using contaminated water for irrigation of crops. These data also demonstrated that some organic solution, as citric acid or vinegar, could be efficient in disinfection of contaminated fruits or vegetable. .
AB  - Kontaminacija voća i povrća može se pojaviti kao rezultat prisustva mikroorganizama kao što su bakterije, virusi i paraziti. Patogene bakterije koje su često prisutne na povrću i voću su: Salmonella spp., Shigella spp., Escherichia coli, E. coli O157:H7, Campylobacter sp., Listeria monocytogenes, Clostridium botulinum, Bacillus cereus. Mikrobna kontaminacija zavisi od različitih faktora koji uključuju zemljišne uslove, vodu za navodnjavanje, organska đubriva, žetvu ili posle žetvene manipulacije. Poznato je nekoliko tipova dekontaminacije koji su delimično efikasni u odstranjivanju patogenih mikroorganizama sa površine celog ili sečenog svežeg voća i povrća. Metode dezinfekcije uključuju tretmane sa hlorom, organskim kiselinama, ozonom, UV zracima. Nekoliko vrsta povrća i voća je testirano na mogućnost dekontaminacije. Kontaminacija biljaka je izvedena držanjem lista zelene salate, ploda paradajza i ploda jabuke u veštački kontaminiranoj vodi. Postupak dekontaminacije se sastojao u dezinfekciji UV zracima i primeni različitih vrsta rastvora kao što su: vinsko sirće, jabukovo sirće, rastvor sirćetne kiseline i limunska kiselina. Brojnost E. coli i Salmonella sp. na površini vrsta povrća i voća je odreden Petri-film metodom (3M, USA). Najveći efekat dekontaminacije, koji je rezultirao prisustvom najmanjeg broja bakterija je dobijen posle tridesetominutnog tretmana lista zelene salate sa limunskom kiselinom. Ovi rezultati ukazuju na potencijalni rizik ako se koristi kontaminirana voda za navodnjavanje useva. Takođe, ovi podaci pokazuju da neka jedinjenja, kao što su rastvor limunske kiseline ili sirće, mogu biti delotvorni u dezinfekciji kontominiranog voća i povrća sa patogenim bakterijama. .
PB  - Unija bioloških naučnih društava Jugoslavije, Beograd
T2  - Zemljište i biljka
T1  - Effect of UV treatment, vinegar and citric acid on removal pathogen bacteria from fruit and vegetables
T1  - Uticaj UV tretmana, sirćeta i limunske kiseline na odstranjivanje patogenih bakterija sa voća i povrća
EP  - 146
IS  - 3
SP  - 139
VL  - 57
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1645
ER  - 

@article{
author = "Kljujev, Igor and Raičević, Vera",
year = "2008",
abstract = "Contamination of fruits and vegetables can arise as a result of presence of microorganisms as bacteria, viruses and parasites. Pathogenic bacteria often found on vegetables and fruits are: Salmonella spp., Shigella spp., Escherichia coli, E. coli O157:H7, Campylobacter sp., Listeria monocytogenes, Clostridium botulinum, Bacillus cereus. Microbial contamination depends on different factors including soil conditions, water for irrigation, organic fertilisers, harvesting or post harvest treatment. Several types' of decontamination treatment are known to be partially effective in removing disease-causing organisms from the surface of whole and cut raw fruits and vegetables or from contact surfaces during han­dling. Disinfection methods include chlorine, organic acid, ozone, UV light. Several vegetable and fruit species were tested for decontamination. The crop contamination was done by keeping lettuce, tomato and apple fruit in artificially contaminated water. Decontamination procedure included disinfection by UV and different organic solutions included: vine vinegar, apple vinegar, vinegar and citric acid. The number of E. coli and Salmonella sp. on the surface of vegetable species was determined by using Petri-film method (3M, USA). The biggest effect of decontamination which resulted in the smallest number of bacteria was obtained after 30 min. of lettuce exposure to citric acid. Microbiological results indirectly pointed out on potential risk of using contaminated water for irrigation of crops. These data also demonstrated that some organic solution, as citric acid or vinegar, could be efficient in disinfection of contaminated fruits or vegetable. ., Kontaminacija voća i povrća može se pojaviti kao rezultat prisustva mikroorganizama kao što su bakterije, virusi i paraziti. Patogene bakterije koje su često prisutne na povrću i voću su: Salmonella spp., Shigella spp., Escherichia coli, E. coli O157:H7, Campylobacter sp., Listeria monocytogenes, Clostridium botulinum, Bacillus cereus. Mikrobna kontaminacija zavisi od različitih faktora koji uključuju zemljišne uslove, vodu za navodnjavanje, organska đubriva, žetvu ili posle žetvene manipulacije. Poznato je nekoliko tipova dekontaminacije koji su delimično efikasni u odstranjivanju patogenih mikroorganizama sa površine celog ili sečenog svežeg voća i povrća. Metode dezinfekcije uključuju tretmane sa hlorom, organskim kiselinama, ozonom, UV zracima. Nekoliko vrsta povrća i voća je testirano na mogućnost dekontaminacije. Kontaminacija biljaka je izvedena držanjem lista zelene salate, ploda paradajza i ploda jabuke u veštački kontaminiranoj vodi. Postupak dekontaminacije se sastojao u dezinfekciji UV zracima i primeni različitih vrsta rastvora kao što su: vinsko sirće, jabukovo sirće, rastvor sirćetne kiseline i limunska kiselina. Brojnost E. coli i Salmonella sp. na površini vrsta povrća i voća je odreden Petri-film metodom (3M, USA). Najveći efekat dekontaminacije, koji je rezultirao prisustvom najmanjeg broja bakterija je dobijen posle tridesetominutnog tretmana lista zelene salate sa limunskom kiselinom. Ovi rezultati ukazuju na potencijalni rizik ako se koristi kontaminirana voda za navodnjavanje useva. Takođe, ovi podaci pokazuju da neka jedinjenja, kao što su rastvor limunske kiseline ili sirće, mogu biti delotvorni u dezinfekciji kontominiranog voća i povrća sa patogenim bakterijama. .",
publisher = "Unija bioloških naučnih društava Jugoslavije, Beograd",
journal = "Zemljište i biljka",
title = "Effect of UV treatment, vinegar and citric acid on removal pathogen bacteria from fruit and vegetables, Uticaj UV tretmana, sirćeta i limunske kiseline na odstranjivanje patogenih bakterija sa voća i povrća",
pages = "146-139",
number = "3",
volume = "57",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1645"
}

Kljujev, I.,& Raičević, V.. (2008). Effect of UV treatment, vinegar and citric acid on removal pathogen bacteria from fruit and vegetables. in Zemljište i biljka
Unija bioloških naučnih društava Jugoslavije, Beograd., 57(3), 139-146.
https://hdl.handle.net/21.15107/rcub_agrospace_1645

Kljujev I, Raičević V. Effect of UV treatment, vinegar and citric acid on removal pathogen bacteria from fruit and vegetables. in Zemljište i biljka. 2008;57(3):139-146.
https://hdl.handle.net/21.15107/rcub_agrospace_1645 .

Kljujev, Igor, Raičević, Vera, "Effect of UV treatment, vinegar and citric acid on removal pathogen bacteria from fruit and vegetables" in Zemljište i biljka, 57, no. 3 (2008):139-146,
https://hdl.handle.net/21.15107/rcub_agrospace_1645 .

TY  - JOUR
AU  - Lalević, Blažo
AU  - Jovanović, Ljubinko
AU  - Raičević, Vera
AU  - Nikšić, Miomir
AU  - Kiković, Dragan
AU  - Marinković, Nataša
AU  - Kljujev, Igor
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1172
AB  - The objective of this research is investigation of Pseudomonas CY growth, isolated from kerosene, in the presence of benzene. Thanks to their high enzymatic activity, microorganisms can use different compounds as a sole sources of carbon and energy, or they can use them in cometabolic processes. Their growth was observed by spectrophotometry measuring optical density on 500nm, while Pseudomonas count was determined on TSA. The growth was observed during specific time period (i.e. in following order: 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12 and 24h). There were used three concentrations of benzene. The results have shown that Pseudomonas population depended on benzene concentration and time. In the presence of benzene the bacterial growth was low, but after 24 hours the Pseudomonas count increased. These results indicate the possibility for Pseudomonas CY growth in the presence of benzene, but also the extension of adaptation phase comparing to normal growth curve. Therefore, the results show that using Pseudomonas population is a very feasible way for decreasing contamination level of soils and waters caused by certain organic compounds.
AB  - Cilj ovog rada je ispitivanje rasta Pseudomonas CY, izolovanog iz kerozina, u prisustvu različitih koncentracija benzena. Mikroorganizmi zahvaljujući svojoj velikoj enzimskoj aktivnosti mogu koristiti različita organska jedinjenja kao jedinstvene izvore ugljenika i energije ili ih koristiti u toku procesa kometabolizma. Rast je praćen metodom spektrofotometrije merenjem optičke gustine na 560 nm a brojnost Pseudomonas bakterija je određena na TSA. Rast je praćen od nultog vremena do 24 sata (l, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12 i 24 h). U radu su korišćene tri koncentracije benzena. Rezultati pokazuju da je brojnost Pseudomonas populacije zavisila od koncentracije benzena ali i vremena. Rast bakterijskih populacija u prisustvu benzena je slabiji, ali nakon 24 sata brojnost se povećava. Ovi rezultati ukazuju da Pseudomonas CY može da raste u prisustvu benzena ali da je faza adaptacije produžena u odnosu na normalnu krivu rasta. Dobijeni rezultati ukazuju na mogućnost korišćenja Pseudomonas populacije za smanjenje stepena kontaminacije zemljišta i voda nekim organskim jedinjenjima.
PB  - Unija bioloških naučnih društava Jugoslavije, Beograd
T2  - Zemljište i biljka
T1  - Pseudomonas CY growth in the presence of benzene
T1  - Rast Pseudomonas CY u prisustvu benzena
EP  - 185
IS  - 3
SP  - 179
VL  - 55
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1172
ER  - 

@article{
author = "Lalević, Blažo and Jovanović, Ljubinko and Raičević, Vera and Nikšić, Miomir and Kiković, Dragan and Marinković, Nataša and Kljujev, Igor",
year = "2006",
abstract = "The objective of this research is investigation of Pseudomonas CY growth, isolated from kerosene, in the presence of benzene. Thanks to their high enzymatic activity, microorganisms can use different compounds as a sole sources of carbon and energy, or they can use them in cometabolic processes. Their growth was observed by spectrophotometry measuring optical density on 500nm, while Pseudomonas count was determined on TSA. The growth was observed during specific time period (i.e. in following order: 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12 and 24h). There were used three concentrations of benzene. The results have shown that Pseudomonas population depended on benzene concentration and time. In the presence of benzene the bacterial growth was low, but after 24 hours the Pseudomonas count increased. These results indicate the possibility for Pseudomonas CY growth in the presence of benzene, but also the extension of adaptation phase comparing to normal growth curve. Therefore, the results show that using Pseudomonas population is a very feasible way for decreasing contamination level of soils and waters caused by certain organic compounds., Cilj ovog rada je ispitivanje rasta Pseudomonas CY, izolovanog iz kerozina, u prisustvu različitih koncentracija benzena. Mikroorganizmi zahvaljujući svojoj velikoj enzimskoj aktivnosti mogu koristiti različita organska jedinjenja kao jedinstvene izvore ugljenika i energije ili ih koristiti u toku procesa kometabolizma. Rast je praćen metodom spektrofotometrije merenjem optičke gustine na 560 nm a brojnost Pseudomonas bakterija je određena na TSA. Rast je praćen od nultog vremena do 24 sata (l, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12 i 24 h). U radu su korišćene tri koncentracije benzena. Rezultati pokazuju da je brojnost Pseudomonas populacije zavisila od koncentracije benzena ali i vremena. Rast bakterijskih populacija u prisustvu benzena je slabiji, ali nakon 24 sata brojnost se povećava. Ovi rezultati ukazuju da Pseudomonas CY može da raste u prisustvu benzena ali da je faza adaptacije produžena u odnosu na normalnu krivu rasta. Dobijeni rezultati ukazuju na mogućnost korišćenja Pseudomonas populacije za smanjenje stepena kontaminacije zemljišta i voda nekim organskim jedinjenjima.",
publisher = "Unija bioloških naučnih društava Jugoslavije, Beograd",
journal = "Zemljište i biljka",
title = "Pseudomonas CY growth in the presence of benzene, Rast Pseudomonas CY u prisustvu benzena",
pages = "185-179",
number = "3",
volume = "55",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1172"
}

Lalević, B., Jovanović, L., Raičević, V., Nikšić, M., Kiković, D., Marinković, N.,& Kljujev, I.. (2006). Pseudomonas CY growth in the presence of benzene. in Zemljište i biljka
Unija bioloških naučnih društava Jugoslavije, Beograd., 55(3), 179-185.
https://hdl.handle.net/21.15107/rcub_agrospace_1172

Lalević B, Jovanović L, Raičević V, Nikšić M, Kiković D, Marinković N, Kljujev I. Pseudomonas CY growth in the presence of benzene. in Zemljište i biljka. 2006;55(3):179-185.
https://hdl.handle.net/21.15107/rcub_agrospace_1172 .

Lalević, Blažo, Jovanović, Ljubinko, Raičević, Vera, Nikšić, Miomir, Kiković, Dragan, Marinković, Nataša, Kljujev, Igor, "Pseudomonas CY growth in the presence of benzene" in Zemljište i biljka, 55, no. 3 (2006):179-185,
https://hdl.handle.net/21.15107/rcub_agrospace_1172 .