TY  - JOUR
AU  - Udovički, Božidar
AU  - Stanković, Slavica
AU  - Tomić, Nikola
AU  - Đekić, Ilija
AU  - Šmigić, Nada
AU  - Špirović Trifunović, Bojana
AU  - Milićević, Dragan
AU  - Rajković, Andreja
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5975
AB  - This study investigated the effectiveness of the ultraviolet irradiation of the C region (UV–C) for the decontamination of Aspergillus flavus and aflatoxin B1 (AFB1) on artificially inoculated maize and peanut using innovative decontamination equipment that supports vibrations to achieve semi-fluidization of the grain/kernel material and getting equal irradiation of all surfaces of irradiated foods. UV irradiation is a non-thermal alternative to thermal processes commonly used in food processing with a well-known effect on AFB1 degradation. Samples of maize and peanut were exposed to UV-C irradiation with a total dose in the range of 1080–8370 mJ/cm2. Analysis by Tracker and ImageJ software confirmed the even distribution of irradiation on all surfaces during the entire duration of exposure. The highest reduction of A. flavus count was observed after ten days of incubation and irradiation treatment delivering a dose of 8370 mJ/cm2 achieving A. flavus count reduction of 4.4 log CFU/g in maize and 3.1 log CFU/g in peanut. Depending on the treatment, AFB1 reduction level in maize ranged from 17 to 43% and in peanut ranged from 14 to 51%. Sensory and physical testing of the peanut samples showed only minimal changes in the evaluated characteristics caused by different levels of the UV-C treatment. Presented results demonstrate a potential for the use of the presented approach as an effective reduction strategy for both A. flavus and AFB1 in maize and peanut.
PB  - Elsevier Ltd
T2  - Food Control
T1  - Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment
VL  - 134
DO  - 10.1016/j.foodcont.2021.108691
ER  - 

@article{
author = "Udovički, Božidar and Stanković, Slavica and Tomić, Nikola and Đekić, Ilija and Šmigić, Nada and Špirović Trifunović, Bojana and Milićević, Dragan and Rajković, Andreja",
year = "2022",
abstract = "This study investigated the effectiveness of the ultraviolet irradiation of the C region (UV–C) for the decontamination of Aspergillus flavus and aflatoxin B1 (AFB1) on artificially inoculated maize and peanut using innovative decontamination equipment that supports vibrations to achieve semi-fluidization of the grain/kernel material and getting equal irradiation of all surfaces of irradiated foods. UV irradiation is a non-thermal alternative to thermal processes commonly used in food processing with a well-known effect on AFB1 degradation. Samples of maize and peanut were exposed to UV-C irradiation with a total dose in the range of 1080–8370 mJ/cm2. Analysis by Tracker and ImageJ software confirmed the even distribution of irradiation on all surfaces during the entire duration of exposure. The highest reduction of A. flavus count was observed after ten days of incubation and irradiation treatment delivering a dose of 8370 mJ/cm2 achieving A. flavus count reduction of 4.4 log CFU/g in maize and 3.1 log CFU/g in peanut. Depending on the treatment, AFB1 reduction level in maize ranged from 17 to 43% and in peanut ranged from 14 to 51%. Sensory and physical testing of the peanut samples showed only minimal changes in the evaluated characteristics caused by different levels of the UV-C treatment. Presented results demonstrate a potential for the use of the presented approach as an effective reduction strategy for both A. flavus and AFB1 in maize and peanut.",
publisher = "Elsevier Ltd",
journal = "Food Control",
title = "Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment",
volume = "134",
doi = "10.1016/j.foodcont.2021.108691"
}

Udovički, B., Stanković, S., Tomić, N., Đekić, I., Šmigić, N., Špirović Trifunović, B., Milićević, D.,& Rajković, A.. (2022). Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment. in Food Control
Elsevier Ltd., 134.
https://doi.org/10.1016/j.foodcont.2021.108691

Udovički B, Stanković S, Tomić N, Đekić I, Šmigić N, Špirović Trifunović B, Milićević D, Rajković A. Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment. in Food Control. 2022;134.
doi:10.1016/j.foodcont.2021.108691 .

Udovički, Božidar, Stanković, Slavica, Tomić, Nikola, Đekić, Ilija, Šmigić, Nada, Špirović Trifunović, Bojana, Milićević, Dragan, Rajković, Andreja, "Evaluation of ultraviolet irradiation effects on Aspergillus flavus and Aflatoxin B1 in maize and peanut using innovative vibrating decontamination equipment" in Food Control, 134 (2022),
https://doi.org/10.1016/j.foodcont.2021.108691 . .

TY  - JOUR
AU  - Nastasijević, Ivan
AU  - Tomašević, Igor
AU  - Šmigić, Nada
AU  - Milićević, Dragan
AU  - Petrović, Zoran
AU  - Djekić, Ilija
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4147
AB  - The recent proposals for modernization of the meat inspection system across the EU recommend that it should be risk-based and also suggest that risk categorization of slaughterhouses should be based on a process hygiene output through the use of indicator organisms - Aerobic Colony Count (ACC) and Enterobacteriaceae count (EC), monitored on carcasses. In this study, the evaluation of the hygiene of operations in cattle and pig slaughterhouses/meat processing companies in Serbia was carried out through two methodologies: a) auditing based on scoring systems used in the UK and Australia; and b) microbial process hygiene indicators. The results confirmed that slaughterhouses with higher levels of hygiene had lower ACC and EC levels. The complementary nature of the methodologies may be effectively used in a process of risk categorization of slaughterhouses. To achieve better consistency and objectiveness in the assessment process, the intensive and structured training of regulatory authorities (official veterinary inspectors, meat inspectors) will be essential.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Hygiene assessment of Serbian meat establishments using different scoring systems
EP  - 200
SP  - 193
VL  - 62
DO  - 10.1016/j.foodcont.2015.10.034
ER  - 

@article{
author = "Nastasijević, Ivan and Tomašević, Igor and Šmigić, Nada and Milićević, Dragan and Petrović, Zoran and Djekić, Ilija",
year = "2016",
abstract = "The recent proposals for modernization of the meat inspection system across the EU recommend that it should be risk-based and also suggest that risk categorization of slaughterhouses should be based on a process hygiene output through the use of indicator organisms - Aerobic Colony Count (ACC) and Enterobacteriaceae count (EC), monitored on carcasses. In this study, the evaluation of the hygiene of operations in cattle and pig slaughterhouses/meat processing companies in Serbia was carried out through two methodologies: a) auditing based on scoring systems used in the UK and Australia; and b) microbial process hygiene indicators. The results confirmed that slaughterhouses with higher levels of hygiene had lower ACC and EC levels. The complementary nature of the methodologies may be effectively used in a process of risk categorization of slaughterhouses. To achieve better consistency and objectiveness in the assessment process, the intensive and structured training of regulatory authorities (official veterinary inspectors, meat inspectors) will be essential.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Hygiene assessment of Serbian meat establishments using different scoring systems",
pages = "200-193",
volume = "62",
doi = "10.1016/j.foodcont.2015.10.034"
}

Nastasijević, I., Tomašević, I., Šmigić, N., Milićević, D., Petrović, Z.,& Djekić, I.. (2016). Hygiene assessment of Serbian meat establishments using different scoring systems. in Food Control
Elsevier Sci Ltd, Oxford., 62, 193-200.
https://doi.org/10.1016/j.foodcont.2015.10.034

Nastasijević I, Tomašević I, Šmigić N, Milićević D, Petrović Z, Djekić I. Hygiene assessment of Serbian meat establishments using different scoring systems. in Food Control. 2016;62:193-200.
doi:10.1016/j.foodcont.2015.10.034 .

Nastasijević, Ivan, Tomašević, Igor, Šmigić, Nada, Milićević, Dragan, Petrović, Zoran, Djekić, Ilija, "Hygiene assessment of Serbian meat establishments using different scoring systems" in Food Control, 62 (2016):193-200,
https://doi.org/10.1016/j.foodcont.2015.10.034 . .

TY  - JOUR
AU  - Milićević, Dragan
AU  - Nikšić, Miomir
AU  - Baltić, Tatjana
AU  - Vranić, D.
AU  - Stefanović, S.
AU  - Janković, S.
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2158
AB  - In order to assess of risk assessment, the aim of this paper was to provide good and detailed insight into the level of contamination of complete feedmixes intended for fattening swine from mycotoxin- producing fungi and mycotoxins (n=18). Isolation and quantitative enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated the number of colonies was determined and thent on the basis of characteristic colonies and microscopic analysis was performed to identify genera and species of moulds. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization of the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS). Total fungal counts (CFU/g) ranged from 0,5×105 do 4×106. From a total samples analysed, seven samples had fungal counts higher than the limit established by Serbian regulations (3×105). During a mycological analysis of complete feedmi×es intended for fattening swine, a total of si× genera and 14 species of moulds were identified of which the most frequent one was of the genus Penicillium (94,4%) while the moulds from Fusarium genere isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a less amount. Polymerase chain reaction (PCR) is a set of 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for poliketid synthase (PKS) from Penicillium species and found that the samples possess PKS sequence. The traditional methods for identification of ochrato×in- producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochrato×in-producing fungi is important for ensuring microbiological quality and safety of feed and food.
PB  - Veterinary World
T2  - Veterinary World
T1  - A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment
EP  - 311
IS  - 7
SP  - 305
VL  - 3
DO  - 10.5455/vetworld.2010.305-311
ER  - 

@article{
author = "Milićević, Dragan and Nikšić, Miomir and Baltić, Tatjana and Vranić, D. and Stefanović, S. and Janković, S.",
year = "2010",
abstract = "In order to assess of risk assessment, the aim of this paper was to provide good and detailed insight into the level of contamination of complete feedmixes intended for fattening swine from mycotoxin- producing fungi and mycotoxins (n=18). Isolation and quantitative enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated the number of colonies was determined and thent on the basis of characteristic colonies and microscopic analysis was performed to identify genera and species of moulds. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization of the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS). Total fungal counts (CFU/g) ranged from 0,5×105 do 4×106. From a total samples analysed, seven samples had fungal counts higher than the limit established by Serbian regulations (3×105). During a mycological analysis of complete feedmi×es intended for fattening swine, a total of si× genera and 14 species of moulds were identified of which the most frequent one was of the genus Penicillium (94,4%) while the moulds from Fusarium genere isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a less amount. Polymerase chain reaction (PCR) is a set of 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for poliketid synthase (PKS) from Penicillium species and found that the samples possess PKS sequence. The traditional methods for identification of ochrato×in- producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochrato×in-producing fungi is important for ensuring microbiological quality and safety of feed and food.",
publisher = "Veterinary World",
journal = "Veterinary World",
title = "A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment",
pages = "311-305",
number = "7",
volume = "3",
doi = "10.5455/vetworld.2010.305-311"
}

Milićević, D., Nikšić, M., Baltić, T., Vranić, D., Stefanović, S.,& Janković, S.. (2010). A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment. in Veterinary World
Veterinary World., 3(7), 305-311.
https://doi.org/10.5455/vetworld.2010.305-311

Milićević D, Nikšić M, Baltić T, Vranić D, Stefanović S, Janković S. A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment. in Veterinary World. 2010;3(7):305-311.
doi:10.5455/vetworld.2010.305-311 .

Milićević, Dragan, Nikšić, Miomir, Baltić, Tatjana, Vranić, D., Stefanović, S., Janković, S., "A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment" in Veterinary World, 3, no. 7 (2010):305-311,
https://doi.org/10.5455/vetworld.2010.305-311 . .

TY  - JOUR
AU  - Milićević, Dragan
AU  - Nikšić, Miomir
AU  - Baltić, Tatjana
AU  - Vranić, Danijela
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2347
AB  - This paper provides a brief review of approaches for the early detection and prevention strategies which have been employed in Serbia for the control of ochratoxogenic fungi and its metabolites in feed in the context of a hazard analysis critical control point (HACCP) framework. During a mycological analysis of complete feedmixes intended for fattening swine (n = 18), a total of six genera and 14 species of moulds were identified. Penicillium was present in considerably more samples than any other genus (94.4%), followed by the genera Fusarium (55.5%) and Paecilomyces (44.4%). Other fungi from the genera Aspergillus (22%), Mucor (11.1%) and Alternaria (5.5%) were represented in a smaller amount. Total fungal counts ranged from 10(5) to 40 x 10(5) c.f.u./g. The mycotoxins deoxynivalenol, ochratoxin A and zearalenone were detected, while aflatoxins were not present. Deoxynivalenol was detected in 10 samples in the concentration range 0.25-2.5 mg/kg. Ochratoxin A and zaralenone were detected in nine and eight samples, respectively, in the concentration range 0.057-0.27 and 0.2-5.0 mg/kg, respectively. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS) using polymerase chain reaction (PCR) applied to a set of 18 isolates. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase (PKS) from Penicillium species and it was found that the samples possessed the PKS sequence. These findings indicate that there may be a risk of animal exposure to mycotoxins through the consumption of mouldy infected feeds.
PB  - Springer, New York
T2  - World Journal of Microbiology & Biotechnology
T1  - Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms
EP  - 1720
IS  - 9
SP  - 1715
VL  - 26
DO  - 10.1007/s11274-010-0341-7
ER  - 

@article{
author = "Milićević, Dragan and Nikšić, Miomir and Baltić, Tatjana and Vranić, Danijela",
year = "2010",
abstract = "This paper provides a brief review of approaches for the early detection and prevention strategies which have been employed in Serbia for the control of ochratoxogenic fungi and its metabolites in feed in the context of a hazard analysis critical control point (HACCP) framework. During a mycological analysis of complete feedmixes intended for fattening swine (n = 18), a total of six genera and 14 species of moulds were identified. Penicillium was present in considerably more samples than any other genus (94.4%), followed by the genera Fusarium (55.5%) and Paecilomyces (44.4%). Other fungi from the genera Aspergillus (22%), Mucor (11.1%) and Alternaria (5.5%) were represented in a smaller amount. Total fungal counts ranged from 10(5) to 40 x 10(5) c.f.u./g. The mycotoxins deoxynivalenol, ochratoxin A and zearalenone were detected, while aflatoxins were not present. Deoxynivalenol was detected in 10 samples in the concentration range 0.25-2.5 mg/kg. Ochratoxin A and zaralenone were detected in nine and eight samples, respectively, in the concentration range 0.057-0.27 and 0.2-5.0 mg/kg, respectively. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS) using polymerase chain reaction (PCR) applied to a set of 18 isolates. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase (PKS) from Penicillium species and it was found that the samples possessed the PKS sequence. These findings indicate that there may be a risk of animal exposure to mycotoxins through the consumption of mouldy infected feeds.",
publisher = "Springer, New York",
journal = "World Journal of Microbiology & Biotechnology",
title = "Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms",
pages = "1720-1715",
number = "9",
volume = "26",
doi = "10.1007/s11274-010-0341-7"
}

Milićević, D., Nikšić, M., Baltić, T.,& Vranić, D.. (2010). Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms. in World Journal of Microbiology & Biotechnology
Springer, New York., 26(9), 1715-1720.
https://doi.org/10.1007/s11274-010-0341-7

Milićević D, Nikšić M, Baltić T, Vranić D. Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms. in World Journal of Microbiology & Biotechnology. 2010;26(9):1715-1720.
doi:10.1007/s11274-010-0341-7 .

Milićević, Dragan, Nikšić, Miomir, Baltić, Tatjana, Vranić, Danijela, "Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms" in World Journal of Microbiology & Biotechnology, 26, no. 9 (2010):1715-1720,
https://doi.org/10.1007/s11274-010-0341-7 . .