TY  - CONF
AU  - Djordjević, Vesna
AU  - Baltić, Tatjana
AU  - Parunović, Nenad
AU  - Simunović, S.
AU  - Tomašević, Igor
AU  - Velebit, Branko
AU  - Ćirić, J.
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4964
AB  - The nitrite content (expressed as NaNO2) was measured in 236 samples of different types of domestic and foreign cooked sausages from the Serbian market, according to standard ISO procedure. The highest content of nitrite (expressed as NaNO2) was found in fine comminuted cooked sausages from foreign producers. Similarly, the coarse comminuted cooked sausages from foreign producers contained higher nitrite compared to domestically produced cooked sausages. According to National and EU Regulations, the maximum allowed nitrate content (expressed as NaNO2) is 150 mg/kg. All tested cooked sausages had nitrite levels below the regulatory limits set by National Regulation and Regulation (EC) No. 601/2014, but the highest nitrite contents were found in cooked sausages from foreign producers.
PB  - IOP Publishing Ltd, Bristol
C3  - 60th International Meat Industry Conference (Meatcon2019)
T1  - The nitrite content in domestic and foreign cooked sausages from the Serbian market
VL  - 333
DO  - 10.1088/1755-1315/333/1/012058
ER  - 

@conference{
author = "Djordjević, Vesna and Baltić, Tatjana and Parunović, Nenad and Simunović, S. and Tomašević, Igor and Velebit, Branko and Ćirić, J.",
year = "2019",
abstract = "The nitrite content (expressed as NaNO2) was measured in 236 samples of different types of domestic and foreign cooked sausages from the Serbian market, according to standard ISO procedure. The highest content of nitrite (expressed as NaNO2) was found in fine comminuted cooked sausages from foreign producers. Similarly, the coarse comminuted cooked sausages from foreign producers contained higher nitrite compared to domestically produced cooked sausages. According to National and EU Regulations, the maximum allowed nitrate content (expressed as NaNO2) is 150 mg/kg. All tested cooked sausages had nitrite levels below the regulatory limits set by National Regulation and Regulation (EC) No. 601/2014, but the highest nitrite contents were found in cooked sausages from foreign producers.",
publisher = "IOP Publishing Ltd, Bristol",
journal = "60th International Meat Industry Conference (Meatcon2019)",
title = "The nitrite content in domestic and foreign cooked sausages from the Serbian market",
volume = "333",
doi = "10.1088/1755-1315/333/1/012058"
}

Djordjević, V., Baltić, T., Parunović, N., Simunović, S., Tomašević, I., Velebit, B.,& Ćirić, J.. (2019). The nitrite content in domestic and foreign cooked sausages from the Serbian market. in 60th International Meat Industry Conference (Meatcon2019)
IOP Publishing Ltd, Bristol., 333.
https://doi.org/10.1088/1755-1315/333/1/012058

Djordjević V, Baltić T, Parunović N, Simunović S, Tomašević I, Velebit B, Ćirić J. The nitrite content in domestic and foreign cooked sausages from the Serbian market. in 60th International Meat Industry Conference (Meatcon2019). 2019;333.
doi:10.1088/1755-1315/333/1/012058 .

Djordjević, Vesna, Baltić, Tatjana, Parunović, Nenad, Simunović, S., Tomašević, Igor, Velebit, Branko, Ćirić, J., "The nitrite content in domestic and foreign cooked sausages from the Serbian market" in 60th International Meat Industry Conference (Meatcon2019), 333 (2019),
https://doi.org/10.1088/1755-1315/333/1/012058 . .

TY  - JOUR
AU  - Milićević, Dragan
AU  - Nikšić, Miomir
AU  - Baltić, Tatjana
AU  - Vranić, D.
AU  - Stefanović, S.
AU  - Janković, S.
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2158
AB  - In order to assess of risk assessment, the aim of this paper was to provide good and detailed insight into the level of contamination of complete feedmixes intended for fattening swine from mycotoxin- producing fungi and mycotoxins (n=18). Isolation and quantitative enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated the number of colonies was determined and thent on the basis of characteristic colonies and microscopic analysis was performed to identify genera and species of moulds. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization of the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS). Total fungal counts (CFU/g) ranged from 0,5×105 do 4×106. From a total samples analysed, seven samples had fungal counts higher than the limit established by Serbian regulations (3×105). During a mycological analysis of complete feedmi×es intended for fattening swine, a total of si× genera and 14 species of moulds were identified of which the most frequent one was of the genus Penicillium (94,4%) while the moulds from Fusarium genere isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a less amount. Polymerase chain reaction (PCR) is a set of 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for poliketid synthase (PKS) from Penicillium species and found that the samples possess PKS sequence. The traditional methods for identification of ochrato×in- producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochrato×in-producing fungi is important for ensuring microbiological quality and safety of feed and food.
PB  - Veterinary World
T2  - Veterinary World
T1  - A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment
EP  - 311
IS  - 7
SP  - 305
VL  - 3
DO  - 10.5455/vetworld.2010.305-311
ER  - 

@article{
author = "Milićević, Dragan and Nikšić, Miomir and Baltić, Tatjana and Vranić, D. and Stefanović, S. and Janković, S.",
year = "2010",
abstract = "In order to assess of risk assessment, the aim of this paper was to provide good and detailed insight into the level of contamination of complete feedmixes intended for fattening swine from mycotoxin- producing fungi and mycotoxins (n=18). Isolation and quantitative enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated the number of colonies was determined and thent on the basis of characteristic colonies and microscopic analysis was performed to identify genera and species of moulds. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization of the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS). Total fungal counts (CFU/g) ranged from 0,5×105 do 4×106. From a total samples analysed, seven samples had fungal counts higher than the limit established by Serbian regulations (3×105). During a mycological analysis of complete feedmi×es intended for fattening swine, a total of si× genera and 14 species of moulds were identified of which the most frequent one was of the genus Penicillium (94,4%) while the moulds from Fusarium genere isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a less amount. Polymerase chain reaction (PCR) is a set of 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for poliketid synthase (PKS) from Penicillium species and found that the samples possess PKS sequence. The traditional methods for identification of ochrato×in- producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochrato×in-producing fungi is important for ensuring microbiological quality and safety of feed and food.",
publisher = "Veterinary World",
journal = "Veterinary World",
title = "A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment",
pages = "311-305",
number = "7",
volume = "3",
doi = "10.5455/vetworld.2010.305-311"
}

Milićević, D., Nikšić, M., Baltić, T., Vranić, D., Stefanović, S.,& Janković, S.. (2010). A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment. in Veterinary World
Veterinary World., 3(7), 305-311.
https://doi.org/10.5455/vetworld.2010.305-311

Milićević D, Nikšić M, Baltić T, Vranić D, Stefanović S, Janković S. A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment. in Veterinary World. 2010;3(7):305-311.
doi:10.5455/vetworld.2010.305-311 .

Milićević, Dragan, Nikšić, Miomir, Baltić, Tatjana, Vranić, D., Stefanović, S., Janković, S., "A survey of occurrence of toxogenic fungi and mycotoxins in pig feed samples-use in evaluation of risk assessment" in Veterinary World, 3, no. 7 (2010):305-311,
https://doi.org/10.5455/vetworld.2010.305-311 . .

TY  - JOUR
AU  - Milićević, Dragan
AU  - Nikšić, Miomir
AU  - Baltić, Tatjana
AU  - Vranić, Danijela
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2347
AB  - This paper provides a brief review of approaches for the early detection and prevention strategies which have been employed in Serbia for the control of ochratoxogenic fungi and its metabolites in feed in the context of a hazard analysis critical control point (HACCP) framework. During a mycological analysis of complete feedmixes intended for fattening swine (n = 18), a total of six genera and 14 species of moulds were identified. Penicillium was present in considerably more samples than any other genus (94.4%), followed by the genera Fusarium (55.5%) and Paecilomyces (44.4%). Other fungi from the genera Aspergillus (22%), Mucor (11.1%) and Alternaria (5.5%) were represented in a smaller amount. Total fungal counts ranged from 10(5) to 40 x 10(5) c.f.u./g. The mycotoxins deoxynivalenol, ochratoxin A and zearalenone were detected, while aflatoxins were not present. Deoxynivalenol was detected in 10 samples in the concentration range 0.25-2.5 mg/kg. Ochratoxin A and zaralenone were detected in nine and eight samples, respectively, in the concentration range 0.057-0.27 and 0.2-5.0 mg/kg, respectively. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS) using polymerase chain reaction (PCR) applied to a set of 18 isolates. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase (PKS) from Penicillium species and it was found that the samples possessed the PKS sequence. These findings indicate that there may be a risk of animal exposure to mycotoxins through the consumption of mouldy infected feeds.
PB  - Springer, New York
T2  - World Journal of Microbiology & Biotechnology
T1  - Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms
EP  - 1720
IS  - 9
SP  - 1715
VL  - 26
DO  - 10.1007/s11274-010-0341-7
ER  - 

@article{
author = "Milićević, Dragan and Nikšić, Miomir and Baltić, Tatjana and Vranić, Danijela",
year = "2010",
abstract = "This paper provides a brief review of approaches for the early detection and prevention strategies which have been employed in Serbia for the control of ochratoxogenic fungi and its metabolites in feed in the context of a hazard analysis critical control point (HACCP) framework. During a mycological analysis of complete feedmixes intended for fattening swine (n = 18), a total of six genera and 14 species of moulds were identified. Penicillium was present in considerably more samples than any other genus (94.4%), followed by the genera Fusarium (55.5%) and Paecilomyces (44.4%). Other fungi from the genera Aspergillus (22%), Mucor (11.1%) and Alternaria (5.5%) were represented in a smaller amount. Total fungal counts ranged from 10(5) to 40 x 10(5) c.f.u./g. The mycotoxins deoxynivalenol, ochratoxin A and zearalenone were detected, while aflatoxins were not present. Deoxynivalenol was detected in 10 samples in the concentration range 0.25-2.5 mg/kg. Ochratoxin A and zaralenone were detected in nine and eight samples, respectively, in the concentration range 0.057-0.27 and 0.2-5.0 mg/kg, respectively. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide synthase gene-PKS) using polymerase chain reaction (PCR) applied to a set of 18 isolates. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase (PKS) from Penicillium species and it was found that the samples possessed the PKS sequence. These findings indicate that there may be a risk of animal exposure to mycotoxins through the consumption of mouldy infected feeds.",
publisher = "Springer, New York",
journal = "World Journal of Microbiology & Biotechnology",
title = "Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms",
pages = "1720-1715",
number = "9",
volume = "26",
doi = "10.1007/s11274-010-0341-7"
}

Milićević, D., Nikšić, M., Baltić, T.,& Vranić, D.. (2010). Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms. in World Journal of Microbiology & Biotechnology
Springer, New York., 26(9), 1715-1720.
https://doi.org/10.1007/s11274-010-0341-7

Milićević D, Nikšić M, Baltić T, Vranić D. Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms. in World Journal of Microbiology & Biotechnology. 2010;26(9):1715-1720.
doi:10.1007/s11274-010-0341-7 .

Milićević, Dragan, Nikšić, Miomir, Baltić, Tatjana, Vranić, Danijela, "Isolation, characterization and evaluation of significant mycoflora and mycotoxins in pig feed from Serbian farms" in World Journal of Microbiology & Biotechnology, 26, no. 9 (2010):1715-1720,
https://doi.org/10.1007/s11274-010-0341-7 . .

TY  - JOUR
AU  - Milićević, Dragan
AU  - Nikšić, Miomir
AU  - Baltić, Tatjana
AU  - Stefanović, Srdjan
AU  - Janković, Saša
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1875
AB  - The aim of this paper was to provide detailed insight into the level of contamination of complete feeding mixtures intended for fattening pigs with mycotoxins-producing fungi and mycotoxins (n = 18). Isolation and enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated, the number of colonies was determined and moulds genera and species identification was carried out using colonies morphological determination and microscopic analysis. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide syntheses gene-PKS). Total fungal counts (CFU/g) ranged from 0,5x105 to 4x106. From a total samples analyzed, seven samples had fungal counts higher than the limit established by Serbian regulations (3x105). During a mycological analysis of complete feeding mixtures intended for fattening pigs, a total of six genera and 14 species of moulds were identified and the most frequent one was genus Penicillium (94,4%). The moulds from genus Fusarium were isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a lesser amount. Polymerase chain reaction (PCR) was performed on 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase gene (PKS) from Penicillium dance and found that the samples possessed PKS sequence. The traditional methods for identification of ochratoxin-producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochratoxin-producing fungi is important for ensuring microbiological quality and safety of feed and food. .
AB  - Radi procene rizika od mikotoksina obavljena su mikološka i mikotoksikološka ispitivanja uzoraka potpunih smeša za ishranu svinja koje potiču iz različitih regiona Srbije (n = 18). Ukupan broj plesni i njihova izolacija u uzorcima potpunih smeša za ishranu svinja, određivan je standardnom mikrobiološkom metodom. Nakon inkubiranja, na osnovu izgleda kolonija i mikroskopske analize obavljena je identifikacija rodova i vrsta plesni. Izolati koji su identifi kovani kao Aspergillus i Penicillium vrste podvrgnuti su molekularnoj karakterizaciji na prisustvo gena odgovornog za sintezu OTA (poliketid sinte taza). Ukupan broj plesni u ispitanim uzorcima bio je od 0,5 × 105 do 4 ×106 (CFU/g). U pogledu ukupnog broja plesni (CFU/g) sedam (38,3 posto) uzoraka bilo je kontaminirano plesnima iznad maksimalno dozvoljene količine (3 × 105). Analizom mikopopulacije uzoraka potpunih smeša za ishranu svinja izolovano je šest rodova i 14 vrsta plesni. Najčešće su izolovane plesni iz roda Penicillium (94,4 posto), zatim Fusarium (55,5 posto) i Paecilomyces (44,4 posto), dok su plesni iz roda Aspergillus (22 posto), Mucor (11,1 posto) i Alternaria (5,5 posto) izolovane u manjem procentu. Lančana reakcija polimeraze (PCR) postavljena je za 18 izolata DNK koje pripadaju rodovima Penicillium i Aspergillus. Sekvence proizvoda PCR reakcije kod tri uzorka poređene su sa nukleotidnim sekvencama gena za poliketid sintetazu (PKS) iz plesni Penicillium i ustanovljeno je da uzorci poseduju PKS sekvencu. Razvoj brzih metoda za determinaciju ohratoksogenih plesni ima za cilj unapređenje sistema kontrole bezbednosti i kvaliteta hrane. U svakoj fazi proizvodnje i prometa hrane može da se spreči, smanji ili eliminiše rizik po krajnjeg korisnika.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment
T1  - Prisustvo plesni i mikotoksina u hrani za ishranu svinja - značaj u proceni rizika
EP  - 270
IS  - 5-6
SP  - 261
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1875
ER  - 

@article{
author = "Milićević, Dragan and Nikšić, Miomir and Baltić, Tatjana and Stefanović, Srdjan and Janković, Saša",
year = "2009",
abstract = "The aim of this paper was to provide detailed insight into the level of contamination of complete feeding mixtures intended for fattening pigs with mycotoxins-producing fungi and mycotoxins (n = 18). Isolation and enumeration of fungal propagules were done on solid media using the standard microbiological procedure. These plates were incubated, the number of colonies was determined and moulds genera and species identification was carried out using colonies morphological determination and microscopic analysis. Isolates identified as Aspergillus and Penicillium species were subjected to molecular characterization for the presence of genes responsible for the synthesis of OTA (polyketide syntheses gene-PKS). Total fungal counts (CFU/g) ranged from 0,5x105 to 4x106. From a total samples analyzed, seven samples had fungal counts higher than the limit established by Serbian regulations (3x105). During a mycological analysis of complete feeding mixtures intended for fattening pigs, a total of six genera and 14 species of moulds were identified and the most frequent one was genus Penicillium (94,4%). The moulds from genus Fusarium were isolated in 55,5% and Paecilomyces in 44,4% of the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%) were represented in a lesser amount. Polymerase chain reaction (PCR) was performed on 18 isolates of the DNA belonging to families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide sequences of genes for polyketide synthase gene (PKS) from Penicillium dance and found that the samples possessed PKS sequence. The traditional methods for identification of ochratoxin-producing fungi are time-consuming and labor-intensive. Rapid and specific detection of ochratoxin-producing fungi is important for ensuring microbiological quality and safety of feed and food. ., Radi procene rizika od mikotoksina obavljena su mikološka i mikotoksikološka ispitivanja uzoraka potpunih smeša za ishranu svinja koje potiču iz različitih regiona Srbije (n = 18). Ukupan broj plesni i njihova izolacija u uzorcima potpunih smeša za ishranu svinja, određivan je standardnom mikrobiološkom metodom. Nakon inkubiranja, na osnovu izgleda kolonija i mikroskopske analize obavljena je identifikacija rodova i vrsta plesni. Izolati koji su identifi kovani kao Aspergillus i Penicillium vrste podvrgnuti su molekularnoj karakterizaciji na prisustvo gena odgovornog za sintezu OTA (poliketid sinte taza). Ukupan broj plesni u ispitanim uzorcima bio je od 0,5 × 105 do 4 ×106 (CFU/g). U pogledu ukupnog broja plesni (CFU/g) sedam (38,3 posto) uzoraka bilo je kontaminirano plesnima iznad maksimalno dozvoljene količine (3 × 105). Analizom mikopopulacije uzoraka potpunih smeša za ishranu svinja izolovano je šest rodova i 14 vrsta plesni. Najčešće su izolovane plesni iz roda Penicillium (94,4 posto), zatim Fusarium (55,5 posto) i Paecilomyces (44,4 posto), dok su plesni iz roda Aspergillus (22 posto), Mucor (11,1 posto) i Alternaria (5,5 posto) izolovane u manjem procentu. Lančana reakcija polimeraze (PCR) postavljena je za 18 izolata DNK koje pripadaju rodovima Penicillium i Aspergillus. Sekvence proizvoda PCR reakcije kod tri uzorka poređene su sa nukleotidnim sekvencama gena za poliketid sintetazu (PKS) iz plesni Penicillium i ustanovljeno je da uzorci poseduju PKS sekvencu. Razvoj brzih metoda za determinaciju ohratoksogenih plesni ima za cilj unapređenje sistema kontrole bezbednosti i kvaliteta hrane. U svakoj fazi proizvodnje i prometa hrane može da se spreči, smanji ili eliminiše rizik po krajnjeg korisnika.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment, Prisustvo plesni i mikotoksina u hrani za ishranu svinja - značaj u proceni rizika",
pages = "270-261",
number = "5-6",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1875"
}

Milićević, D., Nikšić, M., Baltić, T., Stefanović, S.,& Janković, S.. (2009). Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 50(5-6), 261-270.
https://hdl.handle.net/21.15107/rcub_agrospace_1875

Milićević D, Nikšić M, Baltić T, Stefanović S, Janković S. Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment. in Tehnologija mesa. 2009;50(5-6):261-270.
https://hdl.handle.net/21.15107/rcub_agrospace_1875 .

Milićević, Dragan, Nikšić, Miomir, Baltić, Tatjana, Stefanović, Srdjan, Janković, Saša, "Presence of moulds and mycotoxins in pigs' feed: Significance in risk assessment" in Tehnologija mesa, 50, no. 5-6 (2009):261-270,
https://hdl.handle.net/21.15107/rcub_agrospace_1875 .