TY  - JOUR
AU  - Kokina, Mariia
AU  - Salević, Ana
AU  - Kalušević, Ana
AU  - Lević, Steva
AU  - Pantić, Milena
AU  - Pljevljakusić, Dejan
AU  - Savikin, Katarina
AU  - Shamtsyan, Mark
AU  - Nikšić, Miomir
AU  - Nedović, Viktor
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4930
AB  - The study assessed the antimicrobial and antioxidant activities of commonly used and commercially available essential oils as an alternative to synthetic preservatives. The plant sources were as follows: lavender (Lavandula angustifolia), tea tree (Melaleuca alternifolia), bergamot (Citrus bergamia) and peppermint (Mentha piperita). The antioxidant activity of essential oils was tested by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods. The microdilution broth susceptibility assay revealed that lavender and bergamot essential oils were more efficient in inhibiting the bacterial growth than other tested oils, with the minimum inhibitory concentration of 5 mu g/mL. This study also reports the successful implementation of an electrostatic extrusion technique for encapsulating essential oils into alginate beads, which enables the essential oils to maintain their free radical scavenging ability over time.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Characterization, Antioxidant and Antibacterial Activity of Essential Oils and Their Encapsulation into Biodegradable Material Followed by Freeze Drying
EP  - 289
IS  - 2
SP  - 282
VL  - 57
DO  - 10.17113/ftb.57.02.19.5957
ER  - 

@article{
author = "Kokina, Mariia and Salević, Ana and Kalušević, Ana and Lević, Steva and Pantić, Milena and Pljevljakusić, Dejan and Savikin, Katarina and Shamtsyan, Mark and Nikšić, Miomir and Nedović, Viktor",
year = "2019",
abstract = "The study assessed the antimicrobial and antioxidant activities of commonly used and commercially available essential oils as an alternative to synthetic preservatives. The plant sources were as follows: lavender (Lavandula angustifolia), tea tree (Melaleuca alternifolia), bergamot (Citrus bergamia) and peppermint (Mentha piperita). The antioxidant activity of essential oils was tested by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods. The microdilution broth susceptibility assay revealed that lavender and bergamot essential oils were more efficient in inhibiting the bacterial growth than other tested oils, with the minimum inhibitory concentration of 5 mu g/mL. This study also reports the successful implementation of an electrostatic extrusion technique for encapsulating essential oils into alginate beads, which enables the essential oils to maintain their free radical scavenging ability over time.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Characterization, Antioxidant and Antibacterial Activity of Essential Oils and Their Encapsulation into Biodegradable Material Followed by Freeze Drying",
pages = "289-282",
number = "2",
volume = "57",
doi = "10.17113/ftb.57.02.19.5957"
}

Kokina, M., Salević, A., Kalušević, A., Lević, S., Pantić, M., Pljevljakusić, D., Savikin, K., Shamtsyan, M., Nikšić, M.,& Nedović, V.. (2019). Characterization, Antioxidant and Antibacterial Activity of Essential Oils and Their Encapsulation into Biodegradable Material Followed by Freeze Drying. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 57(2), 282-289.
https://doi.org/10.17113/ftb.57.02.19.5957

Kokina M, Salević A, Kalušević A, Lević S, Pantić M, Pljevljakusić D, Savikin K, Shamtsyan M, Nikšić M, Nedović V. Characterization, Antioxidant and Antibacterial Activity of Essential Oils and Their Encapsulation into Biodegradable Material Followed by Freeze Drying. in Food Technology and Biotechnology. 2019;57(2):282-289.
doi:10.17113/ftb.57.02.19.5957 .

Kokina, Mariia, Salević, Ana, Kalušević, Ana, Lević, Steva, Pantić, Milena, Pljevljakusić, Dejan, Savikin, Katarina, Shamtsyan, Mark, Nikšić, Miomir, Nedović, Viktor, "Characterization, Antioxidant and Antibacterial Activity of Essential Oils and Their Encapsulation into Biodegradable Material Followed by Freeze Drying" in Food Technology and Biotechnology, 57, no. 2 (2019):282-289,
https://doi.org/10.17113/ftb.57.02.19.5957 . .

TY  - JOUR
AU  - Pljevljakusić, Dejan
AU  - Bigović, Dubravka
AU  - Janković, Teodora
AU  - Jelačić, Slavica
AU  - Savikin, Katarina
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4770
AB  - Sandy everlasting [Helichrysum arenarium (L.) Moench] is herbaceous perennial plant belonging to Asteraceae family and it is native to Europe, Central Asia, and China. It belongs to the section HELICHRYSUM (Asteraceae family, genus Helichrysum) along with H. plicatum DC. Prodr., which very similar phenolic profile and H. italicum (Roth), which is widely used for essential oil extraction. Its flowers have a long tradition in European ethnomedicine as a cholagogue, choleretic, hepatoprotective, and detoxifying herbal drug. The flowers are rich in phenolic compounds including flavonoids, chalcones, phenolic acids, coumarins, and pyrones. Apart frompolyphenols, other compounds such as sterols, lignans, and glycosides of aromatic compounds have been also isolated from H. arenarium. The majority of authors confirm that the most important group of compounds responsible for biological activities is flavonoids. Moreover, significant activities of naringenin, one of the main flavonoids of H. arenarium, were reported. On the other hand, there are no clinical data about testing the extracts or preparations based on H. arenarium. Although H. arenarium is well known in phytotherapy for its potential in the treatment of gallbladder disease and are classified as endangered species in a number of European countries, very few data about its cultivation are available in the literature.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Sandy Everlasting (Helichrysum arenarium (L.) Moench): Botanical, Chemical and Biological Properties
VL  - 9
DO  - 10.3389/fpls.2018.01123
ER  - 

@article{
author = "Pljevljakusić, Dejan and Bigović, Dubravka and Janković, Teodora and Jelačić, Slavica and Savikin, Katarina",
year = "2018",
abstract = "Sandy everlasting [Helichrysum arenarium (L.) Moench] is herbaceous perennial plant belonging to Asteraceae family and it is native to Europe, Central Asia, and China. It belongs to the section HELICHRYSUM (Asteraceae family, genus Helichrysum) along with H. plicatum DC. Prodr., which very similar phenolic profile and H. italicum (Roth), which is widely used for essential oil extraction. Its flowers have a long tradition in European ethnomedicine as a cholagogue, choleretic, hepatoprotective, and detoxifying herbal drug. The flowers are rich in phenolic compounds including flavonoids, chalcones, phenolic acids, coumarins, and pyrones. Apart frompolyphenols, other compounds such as sterols, lignans, and glycosides of aromatic compounds have been also isolated from H. arenarium. The majority of authors confirm that the most important group of compounds responsible for biological activities is flavonoids. Moreover, significant activities of naringenin, one of the main flavonoids of H. arenarium, were reported. On the other hand, there are no clinical data about testing the extracts or preparations based on H. arenarium. Although H. arenarium is well known in phytotherapy for its potential in the treatment of gallbladder disease and are classified as endangered species in a number of European countries, very few data about its cultivation are available in the literature.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Sandy Everlasting (Helichrysum arenarium (L.) Moench): Botanical, Chemical and Biological Properties",
volume = "9",
doi = "10.3389/fpls.2018.01123"
}

Pljevljakusić, D., Bigović, D., Janković, T., Jelačić, S.,& Savikin, K.. (2018). Sandy Everlasting (Helichrysum arenarium (L.) Moench): Botanical, Chemical and Biological Properties. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fpls.2018.01123

Pljevljakusić D, Bigović D, Janković T, Jelačić S, Savikin K. Sandy Everlasting (Helichrysum arenarium (L.) Moench): Botanical, Chemical and Biological Properties. in Frontiers in Plant Science. 2018;9.
doi:10.3389/fpls.2018.01123 .

Pljevljakusić, Dejan, Bigović, Dubravka, Janković, Teodora, Jelačić, Slavica, Savikin, Katarina, "Sandy Everlasting (Helichrysum arenarium (L.) Moench): Botanical, Chemical and Biological Properties" in Frontiers in Plant Science, 9 (2018),
https://doi.org/10.3389/fpls.2018.01123 . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Pljevljakusić, Dejan
AU  - Ristić, Mihajlo
AU  - Šoštarić, Ivan
AU  - Kresović, Mirjana
AU  - Simić, Ivana
AU  - Vrbničanin, Sava
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3748
AB  - Essential oil yield and composition of three populations of yarrow (Achillea millefolium aggregate) occurring on saline habitats of Serbia was analyzed in relation to its soil site characteristics. The total of 99 compounds of essential oils was determined within three estimated chemotypes: the chamazulene + trans-caryophyllene+-pinene (in population assigned as P1), the lavandulyl acetate+chamazulene + trans-caryophyllene (in P2) and the trans-chrysanthenyl acetate+trans-caryophyllene+germacrene D (in P3). The highest yield of 1.01 % and the lowest of 0.32 % were determined for populations P1 and P3, respectively. The highest total salt content, corresponding with the highest values of ECe and sodium, chloride and sulfate ion concentration was determined for soil of P1 population, which was assessed as solonchak soil, in difference to much lower value of sodium ions, total salts, ECe and pH, all increasing with the soil depth, as determined for samples of P2 and P3 yarrow populations - typical for the solonetz type of salt affected soils. Differences in yield and chemical composition of essential oils were discussed in term of impact of both population's genetic variability and specific environmental conditions. The essential oil quality in all researched yarrow populations accomplishes standards of EU Pharmacopeia 6.0 and thus, sustainable wild collection of yarrow from halophytic habitats could be recommended.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia
EP  - 1352
IS  - 6
SP  - 1343
VL  - 18
DO  - 10.1080/0972060X.2014.884952
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Pljevljakusić, Dejan and Ristić, Mihajlo and Šoštarić, Ivan and Kresović, Mirjana and Simić, Ivana and Vrbničanin, Sava",
year = "2015",
abstract = "Essential oil yield and composition of three populations of yarrow (Achillea millefolium aggregate) occurring on saline habitats of Serbia was analyzed in relation to its soil site characteristics. The total of 99 compounds of essential oils was determined within three estimated chemotypes: the chamazulene + trans-caryophyllene+-pinene (in population assigned as P1), the lavandulyl acetate+chamazulene + trans-caryophyllene (in P2) and the trans-chrysanthenyl acetate+trans-caryophyllene+germacrene D (in P3). The highest yield of 1.01 % and the lowest of 0.32 % were determined for populations P1 and P3, respectively. The highest total salt content, corresponding with the highest values of ECe and sodium, chloride and sulfate ion concentration was determined for soil of P1 population, which was assessed as solonchak soil, in difference to much lower value of sodium ions, total salts, ECe and pH, all increasing with the soil depth, as determined for samples of P2 and P3 yarrow populations - typical for the solonetz type of salt affected soils. Differences in yield and chemical composition of essential oils were discussed in term of impact of both population's genetic variability and specific environmental conditions. The essential oil quality in all researched yarrow populations accomplishes standards of EU Pharmacopeia 6.0 and thus, sustainable wild collection of yarrow from halophytic habitats could be recommended.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia",
pages = "1352-1343",
number = "6",
volume = "18",
doi = "10.1080/0972060X.2014.884952"
}

Dajić-Stevanović, Z., Pljevljakusić, D., Ristić, M., Šoštarić, I., Kresović, M., Simić, I.,& Vrbničanin, S.. (2015). Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 18(6), 1343-1352.
https://doi.org/10.1080/0972060X.2014.884952

Dajić-Stevanović Z, Pljevljakusić D, Ristić M, Šoštarić I, Kresović M, Simić I, Vrbničanin S. Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia. in Journal of Essential Oil Bearing Plants. 2015;18(6):1343-1352.
doi:10.1080/0972060X.2014.884952 .

Dajić-Stevanović, Zora, Pljevljakusić, Dejan, Ristić, Mihajlo, Šoštarić, Ivan, Kresović, Mirjana, Simić, Ivana, Vrbničanin, Sava, "Essential Oil Composition of Achillea millefolium agg. Populations Collected from Saline Habitats in Serbia" in Journal of Essential Oil Bearing Plants, 18, no. 6 (2015):1343-1352,
https://doi.org/10.1080/0972060X.2014.884952 . .

TY  - JOUR
AU  - Pljevljakusić, Dejan
AU  - Janković, Teodora
AU  - Jelačić, Slavica
AU  - Novaković, Miroslav
AU  - Menković, Nebojša
AU  - Beatović, Damir
AU  - Dajić-Stevanović, Zora
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3482
AB  - Arnica (Arnica montana L) is a perennial mountain plant whose flower heads are mainly used for topical treatment of bruises and sprains in phytopharmaceutical preparations such as ointments, creams and gels. In this study, different models of arnica cultivation have been tested in three-year field trial arranged in 2 x 3 x 2 factorial design. Planting date (spring and autumn), fertilizater type (manure, NPK and control) and mode of reproduction (generative and clonal) were induced factors on the trial. The variation of the rosette diameter, flowering stem count and height, flower heads count and diameter, together with flower heads yield and chemical properties were investigated as morphological parameters. Spring planting of clonally propagated plants was showed to be the least successful model due to the low percent of rooted plants and weak shoot development. Other models yielded from 59.8 to 143.6 kg ha(-1) and 116.3 to 258.7 kg ha(-1) flower heads (dw) in the second and third year, respectively. Total sesquiterpene lactones content in flower heads of all tested variants was above the minimum of pharmacopeia quality regulation and ranged from 4.6 to 13.9 mg g(-1) depending on factor levels, while the amounts of two dominant flavonoid compounds quercetin-3-O-glucoside and kaempferol-3-O-glucoside ranged from 7.8 to 13.9 mg g(-1) and from 2.1 to 4.7 mg g(-1), respectively. All induced factors in the field trial significantly influenced variation in both morphological and chemical parameters, where fertilized variants from autumn planting have shown to be superior in flower heads yield with satisfying levels of the secondary metabolites content. Factorial analysis distinguished that half of morphological parameters positively correlated with flower heads yield. First three ranks of sequential classification of cultivation models with desirable properties were captured by variants planted in autumn, while the last rated variants were those from clonal propagation planted in spring. This attempt of arnica domestication in agroecological conditions of western Serbia has proven to be successful in ensuring high quality raw material, providing at the same time answers to the questions that have not been reviewed so far.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Morphological and chemical characterization of Arnica montana L. under different cultivation models
EP  - 244
SP  - 233
VL  - 52
DO  - 10.1016/j.indcrop.2013.10.035
ER  - 

@article{
author = "Pljevljakusić, Dejan and Janković, Teodora and Jelačić, Slavica and Novaković, Miroslav and Menković, Nebojša and Beatović, Damir and Dajić-Stevanović, Zora",
year = "2014",
abstract = "Arnica (Arnica montana L) is a perennial mountain plant whose flower heads are mainly used for topical treatment of bruises and sprains in phytopharmaceutical preparations such as ointments, creams and gels. In this study, different models of arnica cultivation have been tested in three-year field trial arranged in 2 x 3 x 2 factorial design. Planting date (spring and autumn), fertilizater type (manure, NPK and control) and mode of reproduction (generative and clonal) were induced factors on the trial. The variation of the rosette diameter, flowering stem count and height, flower heads count and diameter, together with flower heads yield and chemical properties were investigated as morphological parameters. Spring planting of clonally propagated plants was showed to be the least successful model due to the low percent of rooted plants and weak shoot development. Other models yielded from 59.8 to 143.6 kg ha(-1) and 116.3 to 258.7 kg ha(-1) flower heads (dw) in the second and third year, respectively. Total sesquiterpene lactones content in flower heads of all tested variants was above the minimum of pharmacopeia quality regulation and ranged from 4.6 to 13.9 mg g(-1) depending on factor levels, while the amounts of two dominant flavonoid compounds quercetin-3-O-glucoside and kaempferol-3-O-glucoside ranged from 7.8 to 13.9 mg g(-1) and from 2.1 to 4.7 mg g(-1), respectively. All induced factors in the field trial significantly influenced variation in both morphological and chemical parameters, where fertilized variants from autumn planting have shown to be superior in flower heads yield with satisfying levels of the secondary metabolites content. Factorial analysis distinguished that half of morphological parameters positively correlated with flower heads yield. First three ranks of sequential classification of cultivation models with desirable properties were captured by variants planted in autumn, while the last rated variants were those from clonal propagation planted in spring. This attempt of arnica domestication in agroecological conditions of western Serbia has proven to be successful in ensuring high quality raw material, providing at the same time answers to the questions that have not been reviewed so far.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Morphological and chemical characterization of Arnica montana L. under different cultivation models",
pages = "244-233",
volume = "52",
doi = "10.1016/j.indcrop.2013.10.035"
}

Pljevljakusić, D., Janković, T., Jelačić, S., Novaković, M., Menković, N., Beatović, D.,& Dajić-Stevanović, Z.. (2014). Morphological and chemical characterization of Arnica montana L. under different cultivation models. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 52, 233-244.
https://doi.org/10.1016/j.indcrop.2013.10.035

Pljevljakusić D, Janković T, Jelačić S, Novaković M, Menković N, Beatović D, Dajić-Stevanović Z. Morphological and chemical characterization of Arnica montana L. under different cultivation models. in Industrial Crops and Products. 2014;52:233-244.
doi:10.1016/j.indcrop.2013.10.035 .

Pljevljakusić, Dejan, Janković, Teodora, Jelačić, Slavica, Novaković, Miroslav, Menković, Nebojša, Beatović, Damir, Dajić-Stevanović, Zora, "Morphological and chemical characterization of Arnica montana L. under different cultivation models" in Industrial Crops and Products, 52 (2014):233-244,
https://doi.org/10.1016/j.indcrop.2013.10.035 . .

TY  - JOUR
AU  - Djordjević, Boban
AU  - Pljevljakusić, Dejan
AU  - Savikin, Katarina
AU  - Stević, Tatjana R.
AU  - Bigović, Dubravka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3401
AB  - Dormant buds are recognized as valuable side product of the blackcurrant cultivation. Four blackcurrant varieties cultivated in Serbia, i.e., Ben Sarek, Ometa, Ben Lomond, and Ben Nevis, were evaluated for the content, chemical composition, and antimicrobial activity of their bud essential oils. The oil yields of buds harvested during two different growth periods ranged from 1.2-2.0%, and the variety Ometa had the highest yield among the tested varieties. GC-FID and GC/MS analysis of the oils allowed the identification of eight main components, i.e., -pinene (1.6-5.4%), sabinene (1.9-38.4%), -car-3-ene (13.0-50.7%), -phellandrene (2.9-18.0%), terpinolene (6.6-11.9%), terpinen-4-ol (0.9-6.6%), -caryophyllene (3.8-10.4%), and -humulene (0.2-4.1%). In addition, the similarity degree of the essential-oil compositions of buds harvested from the upper and lower parts of the shrubs was investigated by hierarchical clustering. All essential oils originating from the same genotype were grouped in the same cluster, indicating the reliability of essential oils as chemotaxonomic markers. For more detailed chemotaxonomic investigations, the three compounds with the greatest variance were chosen, i.e., sabinene, -car-3-ene, and -phellandrene, which proved to be efficient for the variety distinction. Factor analysis showed that the essential-oil composition as chemotaxonomic marker in blackcurrants was more reliable for variety Ben Sarek than for variety Ben Nevis. Moreover, it was demonstrated that the essential oils had very strong inhibitory activity against all tested microorganisms. Fungi were more sensitive than bacteria; indeed their growth was completely inhibited at much lower concentrations. In comparison to commercial antibiotics, significantly lower concentrations of the oils were necessary for the complete inhibition of fungal growth.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Essential Oil from Blackcurrant Buds as Chemotaxonomy Marker and Antimicrobial Agent
EP  - 1240
IS  - 8
SP  - 1228
VL  - 11
DO  - 10.1002/cbdv.201400039
ER  - 

@article{
author = "Djordjević, Boban and Pljevljakusić, Dejan and Savikin, Katarina and Stević, Tatjana R. and Bigović, Dubravka",
year = "2014",
abstract = "Dormant buds are recognized as valuable side product of the blackcurrant cultivation. Four blackcurrant varieties cultivated in Serbia, i.e., Ben Sarek, Ometa, Ben Lomond, and Ben Nevis, were evaluated for the content, chemical composition, and antimicrobial activity of their bud essential oils. The oil yields of buds harvested during two different growth periods ranged from 1.2-2.0%, and the variety Ometa had the highest yield among the tested varieties. GC-FID and GC/MS analysis of the oils allowed the identification of eight main components, i.e., -pinene (1.6-5.4%), sabinene (1.9-38.4%), -car-3-ene (13.0-50.7%), -phellandrene (2.9-18.0%), terpinolene (6.6-11.9%), terpinen-4-ol (0.9-6.6%), -caryophyllene (3.8-10.4%), and -humulene (0.2-4.1%). In addition, the similarity degree of the essential-oil compositions of buds harvested from the upper and lower parts of the shrubs was investigated by hierarchical clustering. All essential oils originating from the same genotype were grouped in the same cluster, indicating the reliability of essential oils as chemotaxonomic markers. For more detailed chemotaxonomic investigations, the three compounds with the greatest variance were chosen, i.e., sabinene, -car-3-ene, and -phellandrene, which proved to be efficient for the variety distinction. Factor analysis showed that the essential-oil composition as chemotaxonomic marker in blackcurrants was more reliable for variety Ben Sarek than for variety Ben Nevis. Moreover, it was demonstrated that the essential oils had very strong inhibitory activity against all tested microorganisms. Fungi were more sensitive than bacteria; indeed their growth was completely inhibited at much lower concentrations. In comparison to commercial antibiotics, significantly lower concentrations of the oils were necessary for the complete inhibition of fungal growth.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Essential Oil from Blackcurrant Buds as Chemotaxonomy Marker and Antimicrobial Agent",
pages = "1240-1228",
number = "8",
volume = "11",
doi = "10.1002/cbdv.201400039"
}

Djordjević, B., Pljevljakusić, D., Savikin, K., Stević, T. R.,& Bigović, D.. (2014). Essential Oil from Blackcurrant Buds as Chemotaxonomy Marker and Antimicrobial Agent. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 11(8), 1228-1240.
https://doi.org/10.1002/cbdv.201400039

Djordjević B, Pljevljakusić D, Savikin K, Stević TR, Bigović D. Essential Oil from Blackcurrant Buds as Chemotaxonomy Marker and Antimicrobial Agent. in Chemistry & Biodiversity. 2014;11(8):1228-1240.
doi:10.1002/cbdv.201400039 .

Djordjević, Boban, Pljevljakusić, Dejan, Savikin, Katarina, Stević, Tatjana R., Bigović, Dubravka, "Essential Oil from Blackcurrant Buds as Chemotaxonomy Marker and Antimicrobial Agent" in Chemistry & Biodiversity, 11, no. 8 (2014):1228-1240,
https://doi.org/10.1002/cbdv.201400039 . .

TY  - JOUR
AU  - Djordjević, Boban
AU  - Savikin, Katarina
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Vulić, Todor
AU  - Pljevljakusić, Dejan
AU  - Oparnica, Čedo
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3301
AB  - Thirteen black currant varieties (Ribes nigrum L.) cultured in Serbia were characterized for their pomological properties and chemical composition (total phenolics, total anthocyanins, anthocyanin aglycones, sugars, and vitamin C). The average amount of vitamin C varied from 122.4 to 193.2 mg/100 g fresh weight (FW), while concentration of invert sugars ranged from 6.3% to 11.1%. The highest amounts of total phenolics and anthocyanins were detected in variety Ometa (278.9 mg of gallic acid equivalents per 100 g of FW [mg GAE/100 g FW] and 135.4 mg/100 g, respectively). Quantitative analyses of anthocyanin aglycones in berries were performed using high-performance liquid chromatography, and delphinidin was found to be dominant compound in 11 varieties. Total phenolics and anthocyanins contents decreased during the processing of berry fruits to juices, and the reduction of anthocyanins was more pronounced, 12%-80%. The radical scavenging activity of black currant juices was investigated by 1,1-diphenyl-2-picrylhydrazyl, and the IC50 value ranged from 1.9 to 4.0 mg/mL. Our results also showed that freezing as a way of preservation and storage could save important phytochemicals and health benefits of berries and berry juices. The amount of total phenolics in berries increased during 1 year of storage by 46.09%-171.76% and in juices by even 107.58%, while the amount of total anthocyanins in berries and juices decrease by 5.63%-52.76% and 13.04%-36.82%, respectively.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Biochemical Properties of the Fresh and Frozen Black Currants and Juices
EP  - 81
IS  - 1
SP  - 73
VL  - 16
DO  - 10.1089/jmf.2011.0256
ER  - 

@article{
author = "Djordjević, Boban and Savikin, Katarina and Zdunić, Gordana and Janković, Teodora and Vulić, Todor and Pljevljakusić, Dejan and Oparnica, Čedo",
year = "2013",
abstract = "Thirteen black currant varieties (Ribes nigrum L.) cultured in Serbia were characterized for their pomological properties and chemical composition (total phenolics, total anthocyanins, anthocyanin aglycones, sugars, and vitamin C). The average amount of vitamin C varied from 122.4 to 193.2 mg/100 g fresh weight (FW), while concentration of invert sugars ranged from 6.3% to 11.1%. The highest amounts of total phenolics and anthocyanins were detected in variety Ometa (278.9 mg of gallic acid equivalents per 100 g of FW [mg GAE/100 g FW] and 135.4 mg/100 g, respectively). Quantitative analyses of anthocyanin aglycones in berries were performed using high-performance liquid chromatography, and delphinidin was found to be dominant compound in 11 varieties. Total phenolics and anthocyanins contents decreased during the processing of berry fruits to juices, and the reduction of anthocyanins was more pronounced, 12%-80%. The radical scavenging activity of black currant juices was investigated by 1,1-diphenyl-2-picrylhydrazyl, and the IC50 value ranged from 1.9 to 4.0 mg/mL. Our results also showed that freezing as a way of preservation and storage could save important phytochemicals and health benefits of berries and berry juices. The amount of total phenolics in berries increased during 1 year of storage by 46.09%-171.76% and in juices by even 107.58%, while the amount of total anthocyanins in berries and juices decrease by 5.63%-52.76% and 13.04%-36.82%, respectively.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Biochemical Properties of the Fresh and Frozen Black Currants and Juices",
pages = "81-73",
number = "1",
volume = "16",
doi = "10.1089/jmf.2011.0256"
}

Djordjević, B., Savikin, K., Zdunić, G., Janković, T., Vulić, T., Pljevljakusić, D.,& Oparnica, Č.. (2013). Biochemical Properties of the Fresh and Frozen Black Currants and Juices. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 16(1), 73-81.
https://doi.org/10.1089/jmf.2011.0256

Djordjević B, Savikin K, Zdunić G, Janković T, Vulić T, Pljevljakusić D, Oparnica Č. Biochemical Properties of the Fresh and Frozen Black Currants and Juices. in Journal of Medicinal Food. 2013;16(1):73-81.
doi:10.1089/jmf.2011.0256 .

Djordjević, Boban, Savikin, Katarina, Zdunić, Gordana, Janković, Teodora, Vulić, Todor, Pljevljakusić, Dejan, Oparnica, Čedo, "Biochemical Properties of the Fresh and Frozen Black Currants and Juices" in Journal of Medicinal Food, 16, no. 1 (2013):73-81,
https://doi.org/10.1089/jmf.2011.0256 . .

TY  - JOUR
AU  - Savikin, Katarina
AU  - Djordjević, Boban
AU  - Ristić, Mihailo S.
AU  - Krivokuća-Djokić, Dragana
AU  - Pljevljakusić, Dejan
AU  - Vulić, Todor
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3300
AB  - Currant seeds, a by-product of juice production, are recognized as a valuable source of oil rich in polyunsaturated fatty acids. We have evaluated 28 currant varieties for their oil content and fatty-acid composition. The oil content in the seeds ranged from 18.227.7%, and no statistical difference between varieties of different fruit color were recorded. Furthermore, the estimated oil yields in the field production ranged from 26.4212.4 kg/ha. The GC and GC/MS chemical profiles of the seed oils extracted from all examined varieties were common for currants. Linoleic acid (LA) was the major component, with contents ranging from 32.746.9% of total fatty acids, followed by a-linolenic acid (ALA; 2.932.0 %), oleic acid (OA; 9.819.9%), ?-linolenic acid (GLA; 3.318.5%), palmitic acid (PA; 4.48.1%), stearidonic acid (SDA; 2.24.7%), and stearic acid (SA; 1.22.4%). Quantitative differences in the fatty-acid profiles between varieties of different fruit color were observed. Blackcurrant varieties showed significantly higher contents of LA, GLA, and PA than red and white currant varieties, whereas significantly higher amounts of ALA and OL were detected in the red and white varieties. Cluster analysis based on the chemical oil profiles joined the blackcurrants in one group, while most of the red and white cultivars joined in a second group at the same linkage distance.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Variation in the Fatty-Acid Content in Seeds of Various Black, Red, and White Currant Varieties
EP  - 165
IS  - 1
SP  - 157
VL  - 10
DO  - 10.1002/cbdv.201200223
ER  - 

@article{
author = "Savikin, Katarina and Djordjević, Boban and Ristić, Mihailo S. and Krivokuća-Djokić, Dragana and Pljevljakusić, Dejan and Vulić, Todor",
year = "2013",
abstract = "Currant seeds, a by-product of juice production, are recognized as a valuable source of oil rich in polyunsaturated fatty acids. We have evaluated 28 currant varieties for their oil content and fatty-acid composition. The oil content in the seeds ranged from 18.227.7%, and no statistical difference between varieties of different fruit color were recorded. Furthermore, the estimated oil yields in the field production ranged from 26.4212.4 kg/ha. The GC and GC/MS chemical profiles of the seed oils extracted from all examined varieties were common for currants. Linoleic acid (LA) was the major component, with contents ranging from 32.746.9% of total fatty acids, followed by a-linolenic acid (ALA; 2.932.0 %), oleic acid (OA; 9.819.9%), ?-linolenic acid (GLA; 3.318.5%), palmitic acid (PA; 4.48.1%), stearidonic acid (SDA; 2.24.7%), and stearic acid (SA; 1.22.4%). Quantitative differences in the fatty-acid profiles between varieties of different fruit color were observed. Blackcurrant varieties showed significantly higher contents of LA, GLA, and PA than red and white currant varieties, whereas significantly higher amounts of ALA and OL were detected in the red and white varieties. Cluster analysis based on the chemical oil profiles joined the blackcurrants in one group, while most of the red and white cultivars joined in a second group at the same linkage distance.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Variation in the Fatty-Acid Content in Seeds of Various Black, Red, and White Currant Varieties",
pages = "165-157",
number = "1",
volume = "10",
doi = "10.1002/cbdv.201200223"
}

Savikin, K., Djordjević, B., Ristić, M. S., Krivokuća-Djokić, D., Pljevljakusić, D.,& Vulić, T.. (2013). Variation in the Fatty-Acid Content in Seeds of Various Black, Red, and White Currant Varieties. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 10(1), 157-165.
https://doi.org/10.1002/cbdv.201200223

Savikin K, Djordjević B, Ristić MS, Krivokuća-Djokić D, Pljevljakusić D, Vulić T. Variation in the Fatty-Acid Content in Seeds of Various Black, Red, and White Currant Varieties. in Chemistry & Biodiversity. 2013;10(1):157-165.
doi:10.1002/cbdv.201200223 .

Savikin, Katarina, Djordjević, Boban, Ristić, Mihailo S., Krivokuća-Djokić, Dragana, Pljevljakusić, Dejan, Vulić, Todor, "Variation in the Fatty-Acid Content in Seeds of Various Black, Red, and White Currant Varieties" in Chemistry & Biodiversity, 10, no. 1 (2013):157-165,
https://doi.org/10.1002/cbdv.201200223 . .

TY  - JOUR
AU  - Pljevljakusić, Dejan
AU  - Rančić, Dragana
AU  - Ristić, Mihailo
AU  - Vujisić, Ljubodrag
AU  - Radanović, Dragoja
AU  - Dajić-Stevanović, Zora
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3039
AB  - Arnica montana (L) is a medicinal plant whose flower heads are widely used for the topical treatment of bruises and sprains in phytopharmaceutical preparations. Despite being cultivated for flower heads production for a long time, the quantity and quality of its underground parts have not received much attention. In this study underground parts of a three-year cultivation trial have been estimated for yield potential and chemical composition of essential oil in two consecutive years. Yields of rhizome dry mass were about 208 kg ha(-1) and 647 kg ha(-1), and root dry mass were about 330 kg ha(-1) and 525 kg ha(-1) in the second and third year of growing period, respectively. The chemical composition of the essential oil from root and rhizome, obtained by hydrodistillation, was analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. At the end of second year essential oils from rhizome and root, yielded on average about 4.05% and 1.89%, and at the end of third year, they yielded 2.43% and 1.85%, respectively. The composition of essential oils was independent regarding cultivation trial factors with small differences regarding years. Aromatic hydrocarbons were predominant group of essential oil constituents (about 84% in average) and were identified as thymol derivates. Main individual components were 2,5-dimethoxy-p-cymene, thymol methyl ether and 2,6-diisopropylanisole. Furthermore, histochemical localization of essential oils in rhizome and root was carried out. Secretory structures responsible for essential oil synthesis and accumulation were studied using light, scanning and transmission microscopy. Secretory tissue is localized in the cortical region of the root and rhizome in form of idioblastic secretory cells and secretory cavities, respectively. Histochemical analyses confirmed the lipid nature of secreted material.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil
EP  - 189
SP  - 177
VL  - 39
DO  - 10.1016/j.indcrop.2012.02.030
ER  - 

@article{
author = "Pljevljakusić, Dejan and Rančić, Dragana and Ristić, Mihailo and Vujisić, Ljubodrag and Radanović, Dragoja and Dajić-Stevanović, Zora",
year = "2012",
abstract = "Arnica montana (L) is a medicinal plant whose flower heads are widely used for the topical treatment of bruises and sprains in phytopharmaceutical preparations. Despite being cultivated for flower heads production for a long time, the quantity and quality of its underground parts have not received much attention. In this study underground parts of a three-year cultivation trial have been estimated for yield potential and chemical composition of essential oil in two consecutive years. Yields of rhizome dry mass were about 208 kg ha(-1) and 647 kg ha(-1), and root dry mass were about 330 kg ha(-1) and 525 kg ha(-1) in the second and third year of growing period, respectively. The chemical composition of the essential oil from root and rhizome, obtained by hydrodistillation, was analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. At the end of second year essential oils from rhizome and root, yielded on average about 4.05% and 1.89%, and at the end of third year, they yielded 2.43% and 1.85%, respectively. The composition of essential oils was independent regarding cultivation trial factors with small differences regarding years. Aromatic hydrocarbons were predominant group of essential oil constituents (about 84% in average) and were identified as thymol derivates. Main individual components were 2,5-dimethoxy-p-cymene, thymol methyl ether and 2,6-diisopropylanisole. Furthermore, histochemical localization of essential oils in rhizome and root was carried out. Secretory structures responsible for essential oil synthesis and accumulation were studied using light, scanning and transmission microscopy. Secretory tissue is localized in the cortical region of the root and rhizome in form of idioblastic secretory cells and secretory cavities, respectively. Histochemical analyses confirmed the lipid nature of secreted material.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil",
pages = "189-177",
volume = "39",
doi = "10.1016/j.indcrop.2012.02.030"
}

Pljevljakusić, D., Rančić, D., Ristić, M., Vujisić, L., Radanović, D.,& Dajić-Stevanović, Z.. (2012). Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil. in Industrial Crops and Products
Elsevier, Amsterdam., 39, 177-189.
https://doi.org/10.1016/j.indcrop.2012.02.030

Pljevljakusić D, Rančić D, Ristić M, Vujisić L, Radanović D, Dajić-Stevanović Z. Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil. in Industrial Crops and Products. 2012;39:177-189.
doi:10.1016/j.indcrop.2012.02.030 .

Pljevljakusić, Dejan, Rančić, Dragana, Ristić, Mihailo, Vujisić, Ljubodrag, Radanović, Dragoja, Dajić-Stevanović, Zora, "Rhizome and root yield of the cultivated Arnica montana L., chemical composition and histochemical localization of essential oil" in Industrial Crops and Products, 39 (2012):177-189,
https://doi.org/10.1016/j.indcrop.2012.02.030 . .

TY  - JOUR
AU  - Jug-Dujaković, Marija
AU  - Ristić, Mihailo
AU  - Pljevljakusić, Dejan
AU  - Dajić-Stevanović, Zora
AU  - Liber, Zlatko
AU  - Hancević, Katarina
AU  - Radić, Tomislav
AU  - Satović, Zlatko
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2976
AB  - Essential oils of 25 indigenous populations of Dalmatian sage (Salvia officinalis L.) that represent nearly half of native distribution area of the species were analyzed. Plantlets collected from wild populations were grown in the same field under the same environmental conditions and then sampled for essential-oil analysis. The yield of essential oil ranged from 1.93 to 3.70% with average of 2.83%. Among the 62 compounds detected, eight (cis-thujone, camphor, trans-thujone, 1,8-cineole, beta-pinene, camphene, borneol, and bornyl acetate) formed 78.1387.33% of essential oils of individual populations. Strong positive correlations were observed between camphor and beta-pinene,beta-pinene and borneol, as well as between borneol and bornyl acetate. The strongest negative correlation was detected between camphor and trans-thujone. Principal component analysis (PCA) on the basis of eight main compounds showed that first main component separated populations with high thujone content, from those rich in camphor, while the second component separated populations rich in cis-thujone from those rich in trans-thujone. Cluster analysis (CA) led to the identification of three chemotypes of S. officinalis populations: cis-thujone; trans-tujone, and camphor/beta-pinene/borneol/bornyl acetate. We propose that differences in essential oils of 25 populations are mostly genetically controlled, since potential environmental factors were controlled in this study.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition
EP  - 2323
IS  - 10
SP  - 2309
VL  - 9
DO  - 10.1002/cbdv.201200131
ER  - 

@article{
author = "Jug-Dujaković, Marija and Ristić, Mihailo and Pljevljakusić, Dejan and Dajić-Stevanović, Zora and Liber, Zlatko and Hancević, Katarina and Radić, Tomislav and Satović, Zlatko",
year = "2012",
abstract = "Essential oils of 25 indigenous populations of Dalmatian sage (Salvia officinalis L.) that represent nearly half of native distribution area of the species were analyzed. Plantlets collected from wild populations were grown in the same field under the same environmental conditions and then sampled for essential-oil analysis. The yield of essential oil ranged from 1.93 to 3.70% with average of 2.83%. Among the 62 compounds detected, eight (cis-thujone, camphor, trans-thujone, 1,8-cineole, beta-pinene, camphene, borneol, and bornyl acetate) formed 78.1387.33% of essential oils of individual populations. Strong positive correlations were observed between camphor and beta-pinene,beta-pinene and borneol, as well as between borneol and bornyl acetate. The strongest negative correlation was detected between camphor and trans-thujone. Principal component analysis (PCA) on the basis of eight main compounds showed that first main component separated populations with high thujone content, from those rich in camphor, while the second component separated populations rich in cis-thujone from those rich in trans-thujone. Cluster analysis (CA) led to the identification of three chemotypes of S. officinalis populations: cis-thujone; trans-tujone, and camphor/beta-pinene/borneol/bornyl acetate. We propose that differences in essential oils of 25 populations are mostly genetically controlled, since potential environmental factors were controlled in this study.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition",
pages = "2323-2309",
number = "10",
volume = "9",
doi = "10.1002/cbdv.201200131"
}

Jug-Dujaković, M., Ristić, M., Pljevljakusić, D., Dajić-Stevanović, Z., Liber, Z., Hancević, K., Radić, T.,& Satović, Z.. (2012). High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 9(10), 2309-2323.
https://doi.org/10.1002/cbdv.201200131

Jug-Dujaković M, Ristić M, Pljevljakusić D, Dajić-Stevanović Z, Liber Z, Hancević K, Radić T, Satović Z. High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition. in Chemistry & Biodiversity. 2012;9(10):2309-2323.
doi:10.1002/cbdv.201200131 .

Jug-Dujaković, Marija, Ristić, Mihailo, Pljevljakusić, Dejan, Dajić-Stevanović, Zora, Liber, Zlatko, Hancević, Katarina, Radić, Tomislav, Satović, Zlatko, "High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition" in Chemistry & Biodiversity, 9, no. 10 (2012):2309-2323,
https://doi.org/10.1002/cbdv.201200131 . .