In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit
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2012
Authors
Popović, Milica M.Bulajić, Aleksandra
Ristić, Danijela
Krstić, Branka
Jankova, Ratko M.
Gavrović-Jankulović, Marija
Article (Published version)
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BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent art...ificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.
Keywords:
cysteine protease inhibitor / kiwifruit cystatin / antifungal / plant natural defense / Alternaria radicina / Botrytis cinereaSource:
Journal of the Science of Food and Agriculture, 2012, 92, 15, 3072-3078Publisher:
- Wiley-Blackwell, Hoboken
Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-MESTD-Basic Research (BR or ON)-172049)
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43001)
DOI: 10.1002/jsfa.5728
ISSN: 0022-5142
PubMed: 22653546
WoS: 000310249600019
Scopus: 2-s2.0-84867900945
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Poljoprivredni fakultetTY - JOUR AU - Popović, Milica M. AU - Bulajić, Aleksandra AU - Ristić, Danijela AU - Krstić, Branka AU - Jankova, Ratko M. AU - Gavrović-Jankulović, Marija PY - 2012 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2975 AB - BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules. PB - Wiley-Blackwell, Hoboken T2 - Journal of the Science of Food and Agriculture T1 - In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit EP - 3078 IS - 15 SP - 3072 VL - 92 DO - 10.1002/jsfa.5728 ER -
@article{ author = "Popović, Milica M. and Bulajić, Aleksandra and Ristić, Danijela and Krstić, Branka and Jankova, Ratko M. and Gavrović-Jankulović, Marija", year = "2012", abstract = "BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.", publisher = "Wiley-Blackwell, Hoboken", journal = "Journal of the Science of Food and Agriculture", title = "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit", pages = "3078-3072", number = "15", volume = "92", doi = "10.1002/jsfa.5728" }
Popović, M. M., Bulajić, A., Ristić, D., Krstić, B., Jankova, R. M.,& Gavrović-Jankulović, M.. (2012). In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture Wiley-Blackwell, Hoboken., 92(15), 3072-3078. https://doi.org/10.1002/jsfa.5728
Popović MM, Bulajić A, Ristić D, Krstić B, Jankova RM, Gavrović-Jankulović M. In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture. 2012;92(15):3072-3078. doi:10.1002/jsfa.5728 .
Popović, Milica M., Bulajić, Aleksandra, Ristić, Danijela, Krstić, Branka, Jankova, Ratko M., Gavrović-Jankulović, Marija, "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit" in Journal of the Science of Food and Agriculture, 92, no. 15 (2012):3072-3078, https://doi.org/10.1002/jsfa.5728 . .