In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit
Само за регистроване кориснике
2012
Аутори
Popović, Milica M.Bulajić, Aleksandra
Ristić, Danijela
Krstić, Branka
Jankova, Ratko M.
Gavrović-Jankulović, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent art...ificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.
Кључне речи:
cysteine protease inhibitor / kiwifruit cystatin / antifungal / plant natural defense / Alternaria radicina / Botrytis cinereaИзвор:
Journal of the Science of Food and Agriculture, 2012, 92, 15, 3072-3078Издавач:
- Wiley-Blackwell, Hoboken
Финансирање / пројекти:
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-172049)
- Агробиодиверзитет и коришћење земљишта у Србији: интегрисана процена биодиверзитета кључних група артропода и биљних патогена (RS-43001)
DOI: 10.1002/jsfa.5728
ISSN: 0022-5142
PubMed: 22653546
WoS: 000310249600019
Scopus: 2-s2.0-84867900945
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Popović, Milica M. AU - Bulajić, Aleksandra AU - Ristić, Danijela AU - Krstić, Branka AU - Jankova, Ratko M. AU - Gavrović-Jankulović, Marija PY - 2012 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2975 AB - BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules. PB - Wiley-Blackwell, Hoboken T2 - Journal of the Science of Food and Agriculture T1 - In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit EP - 3078 IS - 15 SP - 3072 VL - 92 DO - 10.1002/jsfa.5728 ER -
@article{ author = "Popović, Milica M. and Bulajić, Aleksandra and Ristić, Danijela and Krstić, Branka and Jankova, Ratko M. and Gavrović-Jankulović, Marija", year = "2012", abstract = "BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.", publisher = "Wiley-Blackwell, Hoboken", journal = "Journal of the Science of Food and Agriculture", title = "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit", pages = "3078-3072", number = "15", volume = "92", doi = "10.1002/jsfa.5728" }
Popović, M. M., Bulajić, A., Ristić, D., Krstić, B., Jankova, R. M.,& Gavrović-Jankulović, M.. (2012). In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture Wiley-Blackwell, Hoboken., 92(15), 3072-3078. https://doi.org/10.1002/jsfa.5728
Popović MM, Bulajić A, Ristić D, Krstić B, Jankova RM, Gavrović-Jankulović M. In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture. 2012;92(15):3072-3078. doi:10.1002/jsfa.5728 .
Popović, Milica M., Bulajić, Aleksandra, Ristić, Danijela, Krstić, Branka, Jankova, Ratko M., Gavrović-Jankulović, Marija, "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit" in Journal of the Science of Food and Agriculture, 92, no. 15 (2012):3072-3078, https://doi.org/10.1002/jsfa.5728 . .