TY  - JOUR
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Lević, Steva
AU  - Pavlović, Vladimir B.
AU  - Stanojević, Slađana P.
AU  - Nedović, Viktor A.
AU  - Pešić, Mirjana B.
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0268005X23008391
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6432
AB  - This study aimed to evaluate the physical (particle size and ζ-potential) and techno-functional properties (emulsifying and foaming) of goat milk powders enriched with grape pomace seed extract (TME), as promising food ingredients in the formulation of functional food. Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) and Raman spectroscopies, along with advanced chemometric tools were employed as well as Scanning Electron Microscopy (SEM) for analyzing TME powders. All powders exhibited a unimodal particle size distribution and ζ-potential values more negative than −36 mV. ATR-FTIR and Raman spectroscopies combined with principal component analysis (PCA) demonstrated distinct separation among skimmed goat milk (M), thermally treated skimmed goat milk (TM), and TME powders in different spectral regions (amide I, II, III, and fingerprint region). This separation resulted from the applied thermal treatment, the presence of phenolic compounds and their complexes with goat milk proteins, and the formation of Maillard reaction products. SEM analysis confirmed the different morphology and shapes of M, TM and TME powders. The 0.1% solutions of M, TM and TME exhibited good emulsifying properties (emulsion activity index and emulsion stability index) but showed poor foaming properties, except for the M sample. Solution concentrations higher than 0.1% for all samples (0.5% and 1.0%) displayed poor techno-functional properties. In summary, a schematic representation of the arrangement of casein micelles in 0.1% M, TM and TME samples, on oil/water and air/water surfaces was provided. The production of TME powders represents an innovative strategy for waste recovery in the production of functional food ingredients with good emulsifying properties.
T2  - Food Hydrocolloids
T2  - Food HydrocolloidsFood Hydrocolloids
T1  - Goat milk powders enriched with grape pomace seed extract: Physical and techno-functional properties
SP  - 109293
VL  - 146
DO  - 10.1016/j.foodhyd.2023.109293
ER  - 

@article{
author = "Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Kolašinac, Stefan and Rac, Vladislav and Banjac, Nebojša and Lađarević, Jelena and Lević, Steva and Pavlović, Vladimir B. and Stanojević, Slađana P. and Nedović, Viktor A. and Pešić, Mirjana B.",
year = "2024",
abstract = "This study aimed to evaluate the physical (particle size and ζ-potential) and techno-functional properties (emulsifying and foaming) of goat milk powders enriched with grape pomace seed extract (TME), as promising food ingredients in the formulation of functional food. Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) and Raman spectroscopies, along with advanced chemometric tools were employed as well as Scanning Electron Microscopy (SEM) for analyzing TME powders. All powders exhibited a unimodal particle size distribution and ζ-potential values more negative than −36 mV. ATR-FTIR and Raman spectroscopies combined with principal component analysis (PCA) demonstrated distinct separation among skimmed goat milk (M), thermally treated skimmed goat milk (TM), and TME powders in different spectral regions (amide I, II, III, and fingerprint region). This separation resulted from the applied thermal treatment, the presence of phenolic compounds and their complexes with goat milk proteins, and the formation of Maillard reaction products. SEM analysis confirmed the different morphology and shapes of M, TM and TME powders. The 0.1% solutions of M, TM and TME exhibited good emulsifying properties (emulsion activity index and emulsion stability index) but showed poor foaming properties, except for the M sample. Solution concentrations higher than 0.1% for all samples (0.5% and 1.0%) displayed poor techno-functional properties. In summary, a schematic representation of the arrangement of casein micelles in 0.1% M, TM and TME samples, on oil/water and air/water surfaces was provided. The production of TME powders represents an innovative strategy for waste recovery in the production of functional food ingredients with good emulsifying properties.",
journal = "Food Hydrocolloids, Food HydrocolloidsFood Hydrocolloids",
title = "Goat milk powders enriched with grape pomace seed extract: Physical and techno-functional properties",
pages = "109293",
volume = "146",
doi = "10.1016/j.foodhyd.2023.109293"
}

Milinčić, D. D., Kostić, A. Ž., Kolašinac, S., Rac, V., Banjac, N., Lađarević, J., Lević, S., Pavlović, V. B., Stanojević, S. P., Nedović, V. A.,& Pešić, M. B.. (2024). Goat milk powders enriched with grape pomace seed extract: Physical and techno-functional properties. in Food Hydrocolloids, 146, 109293.
https://doi.org/10.1016/j.foodhyd.2023.109293

Milinčić DD, Kostić AŽ, Kolašinac S, Rac V, Banjac N, Lađarević J, Lević S, Pavlović VB, Stanojević SP, Nedović VA, Pešić MB. Goat milk powders enriched with grape pomace seed extract: Physical and techno-functional properties. in Food Hydrocolloids. 2024;146:109293.
doi:10.1016/j.foodhyd.2023.109293 .

Milinčić, Danijel D., Kostić, Aleksandar Ž., Kolašinac, Stefan, Rac, Vladislav, Banjac, Nebojša, Lađarević, Jelena, Lević, Steva, Pavlović, Vladimir B., Stanojević, Slađana P., Nedović, Viktor A., Pešić, Mirjana B., "Goat milk powders enriched with grape pomace seed extract: Physical and techno-functional properties" in Food Hydrocolloids, 146 (2024):109293,
https://doi.org/10.1016/j.foodhyd.2023.109293 . .

TY  - JOUR
AU  - Milivojević, Milan
AU  - Popović, Aleksandra
AU  - Pajić-Lijaković, Ivana
AU  - Šoštarić, Ivan
AU  - Kolašinac, Stefan
AU  - Stevanović, Zora Dajić
PY  - 2023
UR  - https://www.mdpi.com/2310-2861/9/8/620
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6422
AB  - Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.
T2  - Gels
T2  - Gels
T1  - Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications
IS  - 8
SP  - 620
VL  - 9
DO  - 10.3390/gels9080620
ER  - 

@article{
author = "Milivojević, Milan and Popović, Aleksandra and Pajić-Lijaković, Ivana and Šoštarić, Ivan and Kolašinac, Stefan and Stevanović, Zora Dajić",
year = "2023",
abstract = "Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.",
journal = "Gels, Gels",
title = "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications",
number = "8",
pages = "620",
volume = "9",
doi = "10.3390/gels9080620"
}

Milivojević, M., Popović, A., Pajić-Lijaković, I., Šoštarić, I., Kolašinac, S.,& Stevanović, Z. D.. (2023). Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels, 9(8), 620.
https://doi.org/10.3390/gels9080620

Milivojević M, Popović A, Pajić-Lijaković I, Šoštarić I, Kolašinac S, Stevanović ZD. Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels. 2023;9(8):620.
doi:10.3390/gels9080620 .

Milivojević, Milan, Popović, Aleksandra, Pajić-Lijaković, Ivana, Šoštarić, Ivan, Kolašinac, Stefan, Stevanović, Zora Dajić, "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications" in Gels, 9, no. 8 (2023):620,
https://doi.org/10.3390/gels9080620 . .

TY  - JOUR
AU  - Lučić, Milica
AU  - Potkonjak, Nebojša
AU  - Sredović Ignjatović, Ivana
AU  - Lević, Steva
AU  - Dajić-Stevanović, Zora
AU  - Kolašinac, Stefan
AU  - Belović, Miona
AU  - Torbica, Aleksandra
AU  - Zlatanović, Ivan
AU  - Pavlović, Vladimir
AU  - Onjia, Antonije
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/13/2468
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6397
AB  - This study investigates the effects of ultrasound, in combination with chemical pretreatments, on the quality attributes (total phenolic and carotenoid content, antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl assay (DPPH)), ferric-reducing ability (FRAP), CIE L* a* b* color, non-enzymatic browning, rehydration ratio, textural and morphological properties) of red pepper subjected to drying (hot air drying or freeze drying). The fractional factorial design was used to assess the impact of factors. The global Derringer desirability function was used to determine the optimal conditions for the best quality attributes of dried pepper. The drying method influenced total phenolic content, a* (redness), and initial rehydration ratio; pretreatment time significantly affected FRAP antiradical activity, a*, chroma and non-browning index, while pH-value had a significant effect on the texture of dried pepper. Non-enzymatic browning was reduced to 72.6%, while the DPPH antioxidant capacity of freeze-dried peppers was enhanced from 4.2% to 71.9%. Ultrasonic pretreatment led to changes in the pepper morphology, while potassium metabisulfite (KMS) was a more effective additive than citric acid.
T2  - Foods
T2  - Foods
T1  - Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum)
IS  - 13
SP  - 2468
VL  - 12
DO  - 10.3390/foods12132468
ER  - 

@article{
author = "Lučić, Milica and Potkonjak, Nebojša and Sredović Ignjatović, Ivana and Lević, Steva and Dajić-Stevanović, Zora and Kolašinac, Stefan and Belović, Miona and Torbica, Aleksandra and Zlatanović, Ivan and Pavlović, Vladimir and Onjia, Antonije",
year = "2023",
abstract = "This study investigates the effects of ultrasound, in combination with chemical pretreatments, on the quality attributes (total phenolic and carotenoid content, antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl assay (DPPH)), ferric-reducing ability (FRAP), CIE L* a* b* color, non-enzymatic browning, rehydration ratio, textural and morphological properties) of red pepper subjected to drying (hot air drying or freeze drying). The fractional factorial design was used to assess the impact of factors. The global Derringer desirability function was used to determine the optimal conditions for the best quality attributes of dried pepper. The drying method influenced total phenolic content, a* (redness), and initial rehydration ratio; pretreatment time significantly affected FRAP antiradical activity, a*, chroma and non-browning index, while pH-value had a significant effect on the texture of dried pepper. Non-enzymatic browning was reduced to 72.6%, while the DPPH antioxidant capacity of freeze-dried peppers was enhanced from 4.2% to 71.9%. Ultrasonic pretreatment led to changes in the pepper morphology, while potassium metabisulfite (KMS) was a more effective additive than citric acid.",
journal = "Foods, Foods",
title = "Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum)",
number = "13",
pages = "2468",
volume = "12",
doi = "10.3390/foods12132468"
}

Lučić, M., Potkonjak, N., Sredović Ignjatović, I., Lević, S., Dajić-Stevanović, Z., Kolašinac, S., Belović, M., Torbica, A., Zlatanović, I., Pavlović, V.,& Onjia, A.. (2023). Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum). in Foods, 12(13), 2468.
https://doi.org/10.3390/foods12132468

Lučić M, Potkonjak N, Sredović Ignjatović I, Lević S, Dajić-Stevanović Z, Kolašinac S, Belović M, Torbica A, Zlatanović I, Pavlović V, Onjia A. Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum). in Foods. 2023;12(13):2468.
doi:10.3390/foods12132468 .

Lučić, Milica, Potkonjak, Nebojša, Sredović Ignjatović, Ivana, Lević, Steva, Dajić-Stevanović, Zora, Kolašinac, Stefan, Belović, Miona, Torbica, Aleksandra, Zlatanović, Ivan, Pavlović, Vladimir, Onjia, Antonije, "Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum)" in Foods, 12, no. 13 (2023):2468,
https://doi.org/10.3390/foods12132468 . .

TY  - CONF
AU  - Kolašinac, Stefan
AU  - Šoštarić, Ivan
AU  - Ćujić, Svetlana
AU  - Bošnjak-Neumüller, Jasna
AU  - Dajić Stevanović, Zora
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6510
AB  - Essential oils (EOs) are complex mixtures of volatile and semivolatile compounds (aldehydes, ketones, alcohols and esters) obtained usually by hydro-distillation from the dry or fresh plant material (seeds, leaves, stems, bark or wood) [1]. Most of the EOs are used in aroma therapy, plant protection (as pesticide), food industry (as food preservatives), perfume industry etc. Because of high demand, EOs are often counterfeited and the quality of EOs is usually controlled by gas chromatography. 
The aim of this paper is to analyze Origanum oil and its counterfeits by Raman spectroscopy. As an adulterant sunflower oil was used. Adulterated essential oils contained 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90 % of sunflower oil. Raman spectra of pure Origano oil and its counterfeits were measured by Witec R300 system equipped with 532 nm laser. Visual inspection of raw spectra led to conclusion that band at 2851 cm-1 is a marker for sunflower oil presence in Origanum oil. However, this band was obvious only when concentration of sunflower oil is higher than, or equal to 10% and could not be seen in lower concentration. In order to reveal presence of this this band in lower concentration, region between 2800 and 3000 cm-1 was subjected to derivatization and then smoothing. Results displayed that Raman spectroscopy in combination with mathematical transformation of spectra can reveal small concentration of unwanted adulterant in Origanum oil.
C3  - 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION
T1  - Raman spectroscopy associated with chemometrics for determining Origanum oil adulteration
EP  - 189
SP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6510
ER  - 

@conference{
author = "Kolašinac, Stefan and Šoštarić, Ivan and Ćujić, Svetlana and Bošnjak-Neumüller, Jasna and Dajić Stevanović, Zora",
year = "2023",
abstract = "Essential oils (EOs) are complex mixtures of volatile and semivolatile compounds (aldehydes, ketones, alcohols and esters) obtained usually by hydro-distillation from the dry or fresh plant material (seeds, leaves, stems, bark or wood) [1]. Most of the EOs are used in aroma therapy, plant protection (as pesticide), food industry (as food preservatives), perfume industry etc. Because of high demand, EOs are often counterfeited and the quality of EOs is usually controlled by gas chromatography. 
The aim of this paper is to analyze Origanum oil and its counterfeits by Raman spectroscopy. As an adulterant sunflower oil was used. Adulterated essential oils contained 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90 % of sunflower oil. Raman spectra of pure Origano oil and its counterfeits were measured by Witec R300 system equipped with 532 nm laser. Visual inspection of raw spectra led to conclusion that band at 2851 cm-1 is a marker for sunflower oil presence in Origanum oil. However, this band was obvious only when concentration of sunflower oil is higher than, or equal to 10% and could not be seen in lower concentration. In order to reveal presence of this this band in lower concentration, region between 2800 and 3000 cm-1 was subjected to derivatization and then smoothing. Results displayed that Raman spectroscopy in combination with mathematical transformation of spectra can reveal small concentration of unwanted adulterant in Origanum oil.",
journal = "5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION",
title = "Raman spectroscopy associated with chemometrics for determining Origanum oil adulteration",
pages = "189-189",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6510"
}

Kolašinac, S., Šoštarić, I., Ćujić, S., Bošnjak-Neumüller, J.,& Dajić Stevanović, Z.. (2023). Raman spectroscopy associated with chemometrics for determining Origanum oil adulteration. in 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION, 189-189.
https://hdl.handle.net/21.15107/rcub_agrospace_6510

Kolašinac S, Šoštarić I, Ćujić S, Bošnjak-Neumüller J, Dajić Stevanović Z. Raman spectroscopy associated with chemometrics for determining Origanum oil adulteration. in 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION. 2023;:189-189.
https://hdl.handle.net/21.15107/rcub_agrospace_6510 .

Kolašinac, Stefan, Šoštarić, Ivan, Ćujić, Svetlana, Bošnjak-Neumüller, Jasna, Dajić Stevanović, Zora, "Raman spectroscopy associated with chemometrics for determining Origanum oil adulteration" in 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION (2023):189-189,
https://hdl.handle.net/21.15107/rcub_agrospace_6510 .

TY  - JOUR
AU  - Jabboury, Zineb El
AU  - Ousaaid, Driss
AU  - Gašić, Uroš
AU  - Janaćković, Peđa
AU  - Stevanovic, Zora Dajic
AU  - Kolašinac, Stefan
AU  - Benjelloun, Meryem
AU  - Ghadraoui, Lahsen El
PY  - 2023
UR  - https://doi.org/10.1007/s42250-023-00747-8
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6416
AB  - Ammi visnaga is a very popular medicinal plant well known for its biological properties afforded by its dense chemical composition highly dependent on environmental factors. The present study was designed to examine the antioxidant potencies using four complementary assays (Total phenolic content (TPC), total antioxidant capacity (TAC), dihydroxycinnamic acid derivative content (HCA), and cupric ion reducing antioxidant capacity (CUPRAC)) as well as the phenolic profile of different parts of Moroccan Ammi visnaga. The obtained results showed that the flower extract registered the highest amount of TPC (49,71 ± 0,12 mg GAE/g) and antioxidant activity TAC (62.07 ± 2.98 mg AAE/g). Concerning the phenolic profile, the most abundant individual phenolic compounds found in high amounts are chlorogenic acid (56.03%, 11.23% and 32.29 for stem, leaves and flowers, respectively), isorhamnetin_3-O-rutinoside (17.86%, 12.16% and 19.01% for stem, leaves and flowers, respectively), isorhamnetin_3-O-glucoside (15.96%, 10.69%, 10.37% for stem, leaves and flowers, respectively), quercetin_3-O-glucoside (1.61%, 21.11% and 2.85% for stem, leaves and flowers, respectively), kaempferol_3-O-glucoside (1.04%, 3.17% and 7.40% for stem, leaves and flowers, respectively) and isorhamnetin (0.57% 7.59% and 2.77% for stem, leaves and flowers, respectively). The outcomes of this study showed that different parts of A. visnaga contain several bioactive compounds with unique biological properties, so the data required further validation using experimental models.
T2  - Chemistry Africa
T2  - Chemistry AfricaChemistry Africa
T1  - Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region
DO  - 10.1007/s42250-023-00747-8
ER  - 

@article{
author = "Jabboury, Zineb El and Ousaaid, Driss and Gašić, Uroš and Janaćković, Peđa and Stevanovic, Zora Dajic and Kolašinac, Stefan and Benjelloun, Meryem and Ghadraoui, Lahsen El",
year = "2023",
abstract = "Ammi visnaga is a very popular medicinal plant well known for its biological properties afforded by its dense chemical composition highly dependent on environmental factors. The present study was designed to examine the antioxidant potencies using four complementary assays (Total phenolic content (TPC), total antioxidant capacity (TAC), dihydroxycinnamic acid derivative content (HCA), and cupric ion reducing antioxidant capacity (CUPRAC)) as well as the phenolic profile of different parts of Moroccan Ammi visnaga. The obtained results showed that the flower extract registered the highest amount of TPC (49,71 ± 0,12 mg GAE/g) and antioxidant activity TAC (62.07 ± 2.98 mg AAE/g). Concerning the phenolic profile, the most abundant individual phenolic compounds found in high amounts are chlorogenic acid (56.03%, 11.23% and 32.29 for stem, leaves and flowers, respectively), isorhamnetin_3-O-rutinoside (17.86%, 12.16% and 19.01% for stem, leaves and flowers, respectively), isorhamnetin_3-O-glucoside (15.96%, 10.69%, 10.37% for stem, leaves and flowers, respectively), quercetin_3-O-glucoside (1.61%, 21.11% and 2.85% for stem, leaves and flowers, respectively), kaempferol_3-O-glucoside (1.04%, 3.17% and 7.40% for stem, leaves and flowers, respectively) and isorhamnetin (0.57% 7.59% and 2.77% for stem, leaves and flowers, respectively). The outcomes of this study showed that different parts of A. visnaga contain several bioactive compounds with unique biological properties, so the data required further validation using experimental models.",
journal = "Chemistry Africa, Chemistry AfricaChemistry Africa",
title = "Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region",
doi = "10.1007/s42250-023-00747-8"
}

Jabboury, Z. E., Ousaaid, D., Gašić, U., Janaćković, P., Stevanovic, Z. D., Kolašinac, S., Benjelloun, M.,& Ghadraoui, L. E.. (2023). Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region. in Chemistry Africa.
https://doi.org/10.1007/s42250-023-00747-8

Jabboury ZE, Ousaaid D, Gašić U, Janaćković P, Stevanovic ZD, Kolašinac S, Benjelloun M, Ghadraoui LE. Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region. in Chemistry Africa. 2023;.
doi:10.1007/s42250-023-00747-8 .

Jabboury, Zineb El, Ousaaid, Driss, Gašić, Uroš, Janaćković, Peđa, Stevanovic, Zora Dajic, Kolašinac, Stefan, Benjelloun, Meryem, Ghadraoui, Lahsen El, "Unraveling the Phytochemical Profile Variability and Antioxidant Activities of Different Parts of Ammi visnaga (L) Collected from Taounate Region" in Chemistry Africa (2023),
https://doi.org/10.1007/s42250-023-00747-8 . .

TY  - CONF
AU  - Dajić Stevanović, Zora
AU  - Kolašinac, Stefan
AU  - Kišgeci, Jan
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6547
AB  - Stefan Kolasinac <stefankolasinac@gmail.com>
22:10 (пре 0 минута)
коме ја

Essential oils (EOs) are complex mixtures of volatile and semivolatile compounds (aldehydes, ketones, alcohols and esters) obtained usually by hydro-distillation from the dry or fresh plant material (seeds, leaves, stems, bark or wood) [1]. Most of the EOs are used in aroma therapy, plant protection (as pesticide), food industry (as food preservatives), perfume industry etc. Because of high demand, EOs are often counterfeited and the quality of EOs is usually controlled by gas chromatography. The aim of this paper is to analyze Origanum oil and its counterfeits by Raman spectroscopy. As an adulterant sunflower oil was used. Adulterated essential oils contained 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90% of sunflower oil. Raman spectra of pure Origano oil and its counterfeits were measured by Witec R300 system equipped with 532 nm laser. Visual inspection of raw spectra led to conclusion that band at 2851 1/cm is a marker for sunflower oil presence in Origanum oil. However, this band was obvious only when concentration of sunflower oil is higher than, or equal to 10% and could not be seen in lower concentration. In order to reveal presence of this this band in lower concentration, region between 2800 and 3000 1/cm was subjected to derivatization and then smoothing. Results displayed that Raman spectrea copy in combination with mathematical transformation of spectra can reveal small concentration of unwanted adulterant in Origanum oil.
C3  - 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION
T1  - Climate change prediction model for plant bioactive compunds: hops (Humulus Lupulus L.) as a model plant far beyond a brewing use
EP  - 31
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6547
ER  - 

@conference{
author = "Dajić Stevanović, Zora and Kolašinac, Stefan and Kišgeci, Jan",
year = "2023",
abstract = "Stefan Kolasinac <stefankolasinac@gmail.com>
22:10 (пре 0 минута)
коме ја

Essential oils (EOs) are complex mixtures of volatile and semivolatile compounds (aldehydes, ketones, alcohols and esters) obtained usually by hydro-distillation from the dry or fresh plant material (seeds, leaves, stems, bark or wood) [1]. Most of the EOs are used in aroma therapy, plant protection (as pesticide), food industry (as food preservatives), perfume industry etc. Because of high demand, EOs are often counterfeited and the quality of EOs is usually controlled by gas chromatography. The aim of this paper is to analyze Origanum oil and its counterfeits by Raman spectroscopy. As an adulterant sunflower oil was used. Adulterated essential oils contained 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90% of sunflower oil. Raman spectra of pure Origano oil and its counterfeits were measured by Witec R300 system equipped with 532 nm laser. Visual inspection of raw spectra led to conclusion that band at 2851 1/cm is a marker for sunflower oil presence in Origanum oil. However, this band was obvious only when concentration of sunflower oil is higher than, or equal to 10% and could not be seen in lower concentration. In order to reveal presence of this this band in lower concentration, region between 2800 and 3000 1/cm was subjected to derivatization and then smoothing. Results displayed that Raman spectrea copy in combination with mathematical transformation of spectra can reveal small concentration of unwanted adulterant in Origanum oil.",
journal = "5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION",
title = "Climate change prediction model for plant bioactive compunds: hops (Humulus Lupulus L.) as a model plant far beyond a brewing use",
pages = "31-30",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6547"
}

Dajić Stevanović, Z., Kolašinac, S.,& Kišgeci, J.. (2023). Climate change prediction model for plant bioactive compunds: hops (Humulus Lupulus L.) as a model plant far beyond a brewing use. in 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION, 30-31.
https://hdl.handle.net/21.15107/rcub_agrospace_6547

Dajić Stevanović Z, Kolašinac S, Kišgeci J. Climate change prediction model for plant bioactive compunds: hops (Humulus Lupulus L.) as a model plant far beyond a brewing use. in 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION. 2023;:30-31.
https://hdl.handle.net/21.15107/rcub_agrospace_6547 .

Dajić Stevanović, Zora, Kolašinac, Stefan, Kišgeci, Jan, "Climate change prediction model for plant bioactive compunds: hops (Humulus Lupulus L.) as a model plant far beyond a brewing use" in 5th INTERNATIONAL CONFERENCE ON NATURAL PRODUCTS UTILIZATION (2023):30-31,
https://hdl.handle.net/21.15107/rcub_agrospace_6547 .

TY  - CONF
AU  - Kilibarda, S.
AU  - Mačukanović- Jocić, M.
AU  - Rančić, D.
AU  - Pećinar, I.
AU  - Šoštarić, I.
AU  - Aćić, S.
AU  - Kolašinac, S.
AU  - Veljović, M.
AU  - Pajić-Lijaković, I.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6548
AB  - The Balkan Peninsula is characterized by a great diversity of flora and vegetation. Moreover, it is native to
a large number of wild plant species containing carotenoids, biologically active compounds, beneficial for
human health. Carotenoid pigments exhibit a great antioxidant and anti-inflammatory effect, in addition to
beneficial impact on eye health, heart, blood vessels, cognitive function and antiaging. Nevertheless, their
role has been scientifically proven in prevention and treatment of cancer.
The aim of the research was to form a database on carotenoid plants of Serbia and the Balkans that is
set up electronically for easy access, management and updating. The research included the analysis and
organization of information on collected plants, as well as literature data related to the traditional use and
storage of plants and plant parts and products rich in carotenoids in fresh, dried or canned state.
Sixty wild plants from more than ten plant families were registered to contain these antioxidant pigments.
Among them three endemic species namely Lilium bosniacum (Beck) Fritsch (lily native to Bosnia and
Herzegovina), Ramonda nathaliae Pančić & Petrović and R. serbica Pančić were inscribed, of which the last
two are Tertiary relicts. The type and level of carotenoids varied depending on the plant species and organs
including leaves, petals, immature and ripe fruits, pulp, seeds, etc. According to database, fruits were the
richest source of these pigments. Reported carotenoid content, included both xanthophylls and carotenes,
whereas lutein and β-carotene were predominantly major compounds in berries and flowers respectively.
Total carotenoid content, determined by HPLC method, was the highest in fresh berries of Rubus fruticosus L.
(440 μg/100g)
C3  - 8th Balkan Botanical Congress
T1  - Overview of carotenoids distribution among wild plant species of the Balkan Peninsula
EP  - 84
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6548
ER  - 

@conference{
author = "Kilibarda, S. and Mačukanović- Jocić, M. and Rančić, D. and Pećinar, I. and Šoštarić, I. and Aćić, S. and Kolašinac, S. and Veljović, M. and Pajić-Lijaković, I.",
year = "2022",
abstract = "The Balkan Peninsula is characterized by a great diversity of flora and vegetation. Moreover, it is native to
a large number of wild plant species containing carotenoids, biologically active compounds, beneficial for
human health. Carotenoid pigments exhibit a great antioxidant and anti-inflammatory effect, in addition to
beneficial impact on eye health, heart, blood vessels, cognitive function and antiaging. Nevertheless, their
role has been scientifically proven in prevention and treatment of cancer.
The aim of the research was to form a database on carotenoid plants of Serbia and the Balkans that is
set up electronically for easy access, management and updating. The research included the analysis and
organization of information on collected plants, as well as literature data related to the traditional use and
storage of plants and plant parts and products rich in carotenoids in fresh, dried or canned state.
Sixty wild plants from more than ten plant families were registered to contain these antioxidant pigments.
Among them three endemic species namely Lilium bosniacum (Beck) Fritsch (lily native to Bosnia and
Herzegovina), Ramonda nathaliae Pančić & Petrović and R. serbica Pančić were inscribed, of which the last
two are Tertiary relicts. The type and level of carotenoids varied depending on the plant species and organs
including leaves, petals, immature and ripe fruits, pulp, seeds, etc. According to database, fruits were the
richest source of these pigments. Reported carotenoid content, included both xanthophylls and carotenes,
whereas lutein and β-carotene were predominantly major compounds in berries and flowers respectively.
Total carotenoid content, determined by HPLC method, was the highest in fresh berries of Rubus fruticosus L.
(440 μg/100g)",
journal = "8th Balkan Botanical Congress",
title = "Overview of carotenoids distribution among wild plant species of the Balkan Peninsula",
pages = "84-84",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6548"
}

Kilibarda, S., Mačukanović- Jocić, M., Rančić, D., Pećinar, I., Šoštarić, I., Aćić, S., Kolašinac, S., Veljović, M.,& Pajić-Lijaković, I.. (2022). Overview of carotenoids distribution among wild plant species of the Balkan Peninsula. in 8th Balkan Botanical Congress, 84-84.
https://hdl.handle.net/21.15107/rcub_agrospace_6548

Kilibarda S, Mačukanović- Jocić M, Rančić D, Pećinar I, Šoštarić I, Aćić S, Kolašinac S, Veljović M, Pajić-Lijaković I. Overview of carotenoids distribution among wild plant species of the Balkan Peninsula. in 8th Balkan Botanical Congress. 2022;:84-84.
https://hdl.handle.net/21.15107/rcub_agrospace_6548 .

Kilibarda, S., Mačukanović- Jocić, M., Rančić, D., Pećinar, I., Šoštarić, I., Aćić, S., Kolašinac, S., Veljović, M., Pajić-Lijaković, I., "Overview of carotenoids distribution among wild plant species of the Balkan Peninsula" in 8th Balkan Botanical Congress (2022):84-84,
https://hdl.handle.net/21.15107/rcub_agrospace_6548 .

TY  - CONF
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Kostić, Aleksandar
AU  - Lević, Steva
AU  - Salević, Ana
AU  - Nedović, Viktor
AU  - Pešić, Mirjana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6870
AB  - The current trend of food innovations is a development of different milk products enriched with phenolic compounds extracted from food by-products. This represents a promising strategy for food waste recovery and to enhance the beneficial health effects of milk-based functional foods. As one of the promising food ingredients can be skim goat milk enriched with grape pomace seed extract due to improved antioxidant properties of goat milk proteins. So, the aim of this study was the Raman analysis of spray-dried powders of skim goat milk, heated goat milk, and heated goat milk enriched with different concentrations of grape pomace seed extract. The whole statistical procedure (pre-processing and PCA) of Raman spectra was performed independently at Amide I (1700-1600 cm-1), Amide II (1600-1500cm-1), and Amide III (1500-1200cm-1) regions. Raman spectra analysis of amide I and II regions showed a separation between heated and unheated milk due to the loadings for 1650-1653 (α-helix), 1661-1663, 1675, and 1686 cm-1 (turn). Furthermore, in the amide I region, the differences between milk enriched with different concentrations of grape pomace seed extract can be observed due to loadings for wavenumbers in the 1612-1644 cm-1 region. These differences can be related to the changes in the parallel and antiparallel β-sheet structures, differences in the vibrations of the amino acid residues of tyrosine, tryptophan, and phenylalanine and the vibrations of ν(C=C) bonds from the aromatic ring of free flavan-3-ols (catechin, epicatechin, epigallocatechin). For the amide III region, the applied heat treatment and interactions of proteins with phenolic compounds distinguished samples in loadings for wavenumber in 1236-1257 and 1267-1304 cm-1 ranges. These differences can be related to the changes in the α-helix and random coil structures. It can be concluded that PCA separation of analyzed powders in amide I and III regions of Raman spectra caused by the applied thermal treatment, presence of free phenolic compounds, and protein-phenolics interactions, can be used for the characterization of milk and milk-phenolics powders.
PB  - University of Novi Sad, Faculty of Technology Novi Sad
C3  - Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (ICAPP)
T1  - APPLICATION OF RAMAN SPECTROSCOPY FOR THE CHARACTERISATION OF MILK AND MILK-PHENOLICS POWDERS
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6870
ER  - 

@conference{
author = "Milinčić, Danijel and Kolašinac, Stefan and Kostić, Aleksandar and Lević, Steva and Salević, Ana and Nedović, Viktor and Pešić, Mirjana",
year = "2022",
abstract = "The current trend of food innovations is a development of different milk products enriched with phenolic compounds extracted from food by-products. This represents a promising strategy for food waste recovery and to enhance the beneficial health effects of milk-based functional foods. As one of the promising food ingredients can be skim goat milk enriched with grape pomace seed extract due to improved antioxidant properties of goat milk proteins. So, the aim of this study was the Raman analysis of spray-dried powders of skim goat milk, heated goat milk, and heated goat milk enriched with different concentrations of grape pomace seed extract. The whole statistical procedure (pre-processing and PCA) of Raman spectra was performed independently at Amide I (1700-1600 cm-1), Amide II (1600-1500cm-1), and Amide III (1500-1200cm-1) regions. Raman spectra analysis of amide I and II regions showed a separation between heated and unheated milk due to the loadings for 1650-1653 (α-helix), 1661-1663, 1675, and 1686 cm-1 (turn). Furthermore, in the amide I region, the differences between milk enriched with different concentrations of grape pomace seed extract can be observed due to loadings for wavenumbers in the 1612-1644 cm-1 region. These differences can be related to the changes in the parallel and antiparallel β-sheet structures, differences in the vibrations of the amino acid residues of tyrosine, tryptophan, and phenylalanine and the vibrations of ν(C=C) bonds from the aromatic ring of free flavan-3-ols (catechin, epicatechin, epigallocatechin). For the amide III region, the applied heat treatment and interactions of proteins with phenolic compounds distinguished samples in loadings for wavenumber in 1236-1257 and 1267-1304 cm-1 ranges. These differences can be related to the changes in the α-helix and random coil structures. It can be concluded that PCA separation of analyzed powders in amide I and III regions of Raman spectra caused by the applied thermal treatment, presence of free phenolic compounds, and protein-phenolics interactions, can be used for the characterization of milk and milk-phenolics powders.",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad",
journal = "Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (ICAPP)",
title = "APPLICATION OF RAMAN SPECTROSCOPY FOR THE CHARACTERISATION OF MILK AND MILK-PHENOLICS POWDERS",
pages = "98",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6870"
}

Milinčić, D., Kolašinac, S., Kostić, A., Lević, S., Salević, A., Nedović, V.,& Pešić, M.. (2022). APPLICATION OF RAMAN SPECTROSCOPY FOR THE CHARACTERISATION OF MILK AND MILK-PHENOLICS POWDERS. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (ICAPP)
University of Novi Sad, Faculty of Technology Novi Sad., 98.
https://hdl.handle.net/21.15107/rcub_agrospace_6870

Milinčić D, Kolašinac S, Kostić A, Lević S, Salević A, Nedović V, Pešić M. APPLICATION OF RAMAN SPECTROSCOPY FOR THE CHARACTERISATION OF MILK AND MILK-PHENOLICS POWDERS. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (ICAPP). 2022;:98.
https://hdl.handle.net/21.15107/rcub_agrospace_6870 .

Milinčić, Danijel, Kolašinac, Stefan, Kostić, Aleksandar, Lević, Steva, Salević, Ana, Nedović, Viktor, Pešić, Mirjana, "APPLICATION OF RAMAN SPECTROSCOPY FOR THE CHARACTERISATION OF MILK AND MILK-PHENOLICS POWDERS" in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (ICAPP) (2022):98,
https://hdl.handle.net/21.15107/rcub_agrospace_6870 .

TY  - JOUR
AU  - Gordanić, Stefan
AU  - Radanović, Dragoja
AU  - Vuković, Sandra
AU  - Kolašinac, Stefan
AU  - Kilibarda, Sofija
AU  - Marković, Tatjana
AU  - Moravčević, Đorđe
AU  - Kostić, Aleksandar Ž.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6276
AB  - Wild garlic (Allium ursinum L.) has been used as nutrition and medicine for centuries. Although this plant species is a typical spring geophyte that grows spontaneously in moist, steep, shady beech forests, but information on phytochemical and antioxidant properties under various soil types are scarce. This study aimed to assess the phytochemical composition and antioxidant potential of the leaves of A. ursinum grown on different soil types, but under identical climatic conditions of South Banat, Serbia. For the purpose of reproduction, A. ursinum bulbs were collected from two different locations in Serbia and then planted on different types of soil, namely: Arenosol, Fluvisol, Cambisol and Chernozem. Fresh leaves of sprouted plants were sampled at the beginning of spring, morphologically analysed and stoma was counted. The leaf extract was prepared and its phytochemical composition and antioxidant potential were assessed. Regardless of the origin of the reproductive material (bulbs), the leaves of A. ursinum plants cultivated in Chernozem soil had the best morphological characteristics and the largest number of stomata. Phytochemical analyses revealed the following ranges for selected bioactive compounds (expressed on fresh weight, FW): chlorophyll content (289.9-642.4 μg/g for chlorophyll a i.e. 358.2-458.6 μg/g for chlorophyll b), total carotenoid content (TCC, 91.2-263.2 μg/g), total phenolic content (TPC, 1.43-1.98 mg/g GAE), total flavonoid content (TFC, 0.36-1.28 mg/g QE), and total dihydroxycinnamic acid derivative content (HCA, 0.53-0.59 mg/g CGAE). The highest values were obtained on Chernozem (chlorophyll a, chlorophyll b and TPC and HCA) and Cambisol (TCC and TFC). Chernozem appeared to be the best soil type during three applied standard antioxidant assays (CUPRAC, TAC and FRP) while DPPH radical quenching assay revealed no significant differences among all examined soil types. Based on the obtained results it could be assumed that Chernozem exhibited the most desirable physico-chemical properties for optimal development of A. ursinum (in particular its green parts) as a source of different antioxidants. Correlation analysis of phytochemical parameters has proved significant influence of total chlorophylls, phenolics, flavonoids and duhydroxycinnamic acid derivatives on antioxidant activity of A. ursinum leaves (unlike total carotenoid content) with the highest correlation between HCA and FRP assay (r2= 1.00). In addition, PCA analysis clearly determined Chernozem type of soil as the best choice for optimal leaf growth and development. © 2023,Journal of Industrial Engineering and Engineering Management. All Rights Reserved.
T2  - Emirates Journal of Food and Agriculture
T2  - Emirates Journal of Food and Agriculture
T1  - Phytochemical characterization and antioxidant potential of Allium ursinum L. cultivated on different soil types-a preliminary study
EP  - 914
IS  - 11
SP  - 904
VL  - 34
DO  - 10.9755/ejfa.2022.v34.i11.2958
ER  - 

@article{
author = "Gordanić, Stefan and Radanović, Dragoja and Vuković, Sandra and Kolašinac, Stefan and Kilibarda, Sofija and Marković, Tatjana and Moravčević, Đorđe and Kostić, Aleksandar Ž.",
year = "2022",
abstract = "Wild garlic (Allium ursinum L.) has been used as nutrition and medicine for centuries. Although this plant species is a typical spring geophyte that grows spontaneously in moist, steep, shady beech forests, but information on phytochemical and antioxidant properties under various soil types are scarce. This study aimed to assess the phytochemical composition and antioxidant potential of the leaves of A. ursinum grown on different soil types, but under identical climatic conditions of South Banat, Serbia. For the purpose of reproduction, A. ursinum bulbs were collected from two different locations in Serbia and then planted on different types of soil, namely: Arenosol, Fluvisol, Cambisol and Chernozem. Fresh leaves of sprouted plants were sampled at the beginning of spring, morphologically analysed and stoma was counted. The leaf extract was prepared and its phytochemical composition and antioxidant potential were assessed. Regardless of the origin of the reproductive material (bulbs), the leaves of A. ursinum plants cultivated in Chernozem soil had the best morphological characteristics and the largest number of stomata. Phytochemical analyses revealed the following ranges for selected bioactive compounds (expressed on fresh weight, FW): chlorophyll content (289.9-642.4 μg/g for chlorophyll a i.e. 358.2-458.6 μg/g for chlorophyll b), total carotenoid content (TCC, 91.2-263.2 μg/g), total phenolic content (TPC, 1.43-1.98 mg/g GAE), total flavonoid content (TFC, 0.36-1.28 mg/g QE), and total dihydroxycinnamic acid derivative content (HCA, 0.53-0.59 mg/g CGAE). The highest values were obtained on Chernozem (chlorophyll a, chlorophyll b and TPC and HCA) and Cambisol (TCC and TFC). Chernozem appeared to be the best soil type during three applied standard antioxidant assays (CUPRAC, TAC and FRP) while DPPH radical quenching assay revealed no significant differences among all examined soil types. Based on the obtained results it could be assumed that Chernozem exhibited the most desirable physico-chemical properties for optimal development of A. ursinum (in particular its green parts) as a source of different antioxidants. Correlation analysis of phytochemical parameters has proved significant influence of total chlorophylls, phenolics, flavonoids and duhydroxycinnamic acid derivatives on antioxidant activity of A. ursinum leaves (unlike total carotenoid content) with the highest correlation between HCA and FRP assay (r2= 1.00). In addition, PCA analysis clearly determined Chernozem type of soil as the best choice for optimal leaf growth and development. © 2023,Journal of Industrial Engineering and Engineering Management. All Rights Reserved.",
journal = "Emirates Journal of Food and Agriculture, Emirates Journal of Food and Agriculture",
title = "Phytochemical characterization and antioxidant potential of Allium ursinum L. cultivated on different soil types-a preliminary study",
pages = "914-904",
number = "11",
volume = "34",
doi = "10.9755/ejfa.2022.v34.i11.2958"
}

Gordanić, S., Radanović, D., Vuković, S., Kolašinac, S., Kilibarda, S., Marković, T., Moravčević, Đ.,& Kostić, A. Ž.. (2022). Phytochemical characterization and antioxidant potential of Allium ursinum L. cultivated on different soil types-a preliminary study. in Emirates Journal of Food and Agriculture, 34(11), 904-914.
https://doi.org/10.9755/ejfa.2022.v34.i11.2958

Gordanić S, Radanović D, Vuković S, Kolašinac S, Kilibarda S, Marković T, Moravčević Đ, Kostić AŽ. Phytochemical characterization and antioxidant potential of Allium ursinum L. cultivated on different soil types-a preliminary study. in Emirates Journal of Food and Agriculture. 2022;34(11):904-914.
doi:10.9755/ejfa.2022.v34.i11.2958 .

Gordanić, Stefan, Radanović, Dragoja, Vuković, Sandra, Kolašinac, Stefan, Kilibarda, Sofija, Marković, Tatjana, Moravčević, Đorđe, Kostić, Aleksandar Ž., "Phytochemical characterization and antioxidant potential of Allium ursinum L. cultivated on different soil types-a preliminary study" in Emirates Journal of Food and Agriculture, 34, no. 11 (2022):904-914,
https://doi.org/10.9755/ejfa.2022.v34.i11.2958 . .

TY  - CONF
AU  - Žugić Petrović, Tanja
AU  - Mladenović, Katarina
AU  - Grujović, Mirjana
AU  - Kolašinac, Stefan
AU  - Orović, Dragan
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6551
AB  - Today, special attention is paid to organic honey due to increasing pollution
and numerous toxins, and certified organic honey is described as having no chemical
pollution, including that related to bee migration in search of good flowers that
beekeepers do not directly control. This study aimed to characterize through analysis
5 samples of organic sunflower honey from Banat (Serbia): physicochemical quality
parameters (moisture, HMF, diastase activity, free acidity, glucose and fructose
content, sucrose and ash) and microbiological safety (presence of aerobic mesophilic
bacteria, coliforms, lactic acid bacteria, molds and yeasts). Mean values obtained for
physicochemical parameters are: 16.52% humidity, 8.17 mg/kg HMF, 10.8 diastasis
activity, 24.07 meq/kg of free acidity, 60.8% of glucose and fructose 0.5% sucrose
and 0.01% ash. The microbiota that was isolated from all the samples tested
consisted of Bacillus spp. ranging from 0.50-0.55 x10-2 cfu/g and Saccharomyces
spp., ranging from 0.31-0.44 x10-2 cfu/g. MALDI TOF confirmed preliminary
identification to be Bacillus pumilus, while yeasts were identified as Saccharomyces
cerevisiae. This research could contribute to the valorization of sunflower honey,
which would lead to standardization and increase the production of this product.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
T1  - Organic sunflower honey from the area of Banat (northeastern Serbia) - physicochemical and microbiological characterizations
EP  - 111
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6551
ER  - 

@conference{
author = "Žugić Petrović, Tanja and Mladenović, Katarina and Grujović, Mirjana and Kolašinac, Stefan and Orović, Dragan",
year = "2022",
abstract = "Today, special attention is paid to organic honey due to increasing pollution
and numerous toxins, and certified organic honey is described as having no chemical
pollution, including that related to bee migration in search of good flowers that
beekeepers do not directly control. This study aimed to characterize through analysis
5 samples of organic sunflower honey from Banat (Serbia): physicochemical quality
parameters (moisture, HMF, diastase activity, free acidity, glucose and fructose
content, sucrose and ash) and microbiological safety (presence of aerobic mesophilic
bacteria, coliforms, lactic acid bacteria, molds and yeasts). Mean values obtained for
physicochemical parameters are: 16.52% humidity, 8.17 mg/kg HMF, 10.8 diastasis
activity, 24.07 meq/kg of free acidity, 60.8% of glucose and fructose 0.5% sucrose
and 0.01% ash. The microbiota that was isolated from all the samples tested
consisted of Bacillus spp. ranging from 0.50-0.55 x10-2 cfu/g and Saccharomyces
spp., ranging from 0.31-0.44 x10-2 cfu/g. MALDI TOF confirmed preliminary
identification to be Bacillus pumilus, while yeasts were identified as Saccharomyces
cerevisiae. This research could contribute to the valorization of sunflower honey,
which would lead to standardization and increase the production of this product.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions",
title = "Organic sunflower honey from the area of Banat (northeastern Serbia) - physicochemical and microbiological characterizations",
pages = "111-111",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6551"
}

Žugić Petrović, T., Mladenović, K., Grujović, M., Kolašinac, S.,& Orović, D.. (2022). Organic sunflower honey from the area of Banat (northeastern Serbia) - physicochemical and microbiological characterizations. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 111-111.
https://hdl.handle.net/21.15107/rcub_agrospace_6551

Žugić Petrović T, Mladenović K, Grujović M, Kolašinac S, Orović D. Organic sunflower honey from the area of Banat (northeastern Serbia) - physicochemical and microbiological characterizations. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions. 2022;:111-111.
https://hdl.handle.net/21.15107/rcub_agrospace_6551 .

Žugić Petrović, Tanja, Mladenović, Katarina, Grujović, Mirjana, Kolašinac, Stefan, Orović, Dragan, "Organic sunflower honey from the area of Banat (northeastern Serbia) - physicochemical and microbiological characterizations" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions (2022):111-111,
https://hdl.handle.net/21.15107/rcub_agrospace_6551 .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Jelača, Sanja
AU  - Vuković, Nenad
AU  - Kolašinac, Stefan
AU  - Trendafilova, Antoaneta
AU  - Nedialkov, Paraskev
AU  - Stanković, Miroslava
AU  - Tanić, Nasta
AU  - Tanić, Nikola T.
AU  - Acović, Aleksandar
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6239
AB  - Alchemilla vulgaris L. (lady’s mantle) was used for centuries in Europe and Balkan countries for treatments of numerous conditions and diseases of the reproductive system, yet some of the biological activities of lady’s mantle have been poorly studied and neglected. The present study aimed to estimate the potential of A. vulgaris ethanolic extract from Southeast Serbia to prevent and suppress tumor development in vitro, validated by antioxidant, genoprotective, and cytotoxic properties. A total of 45 compounds were detected by UHPLC–HRMS analysis in A. vulgaris ethanolic extract. Measurement of antioxidant activity revealed the significant potential of the tested extract to scavenge free radicals. In addition, the analysis of micronuclei showed an in vitro protective effect on chromosome aberrations in peripheral human lymphocytes. A. vulgaris extract strongly suppressed the growth of human cell lines derived from different types of tumors (MCF-7, A375, A549, and HCT116). The observed antitumor effect is realized through the blockade of cell division, caspase-dependent apoptosis, and autophagic cell death. Our study has shown that Alchemilla vulgaris L. is a valuable source of bioactive compounds able to protect the subcellular structure from damage, thus preventing tumorigenesis as well as suppressing tumor cell growth. © 2022 by the authors.
T2  - Molecules
T1  - Beyond Traditional Use of Alchemilla vulgaris: Genoprotective and Antitumor Activity In Vitro
IS  - 23
VL  - 27
DO  - 10.3390/molecules27238113
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Jelača, Sanja and Vuković, Nenad and Kolašinac, Stefan and Trendafilova, Antoaneta and Nedialkov, Paraskev and Stanković, Miroslava and Tanić, Nasta and Tanić, Nikola T. and Acović, Aleksandar and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "Alchemilla vulgaris L. (lady’s mantle) was used for centuries in Europe and Balkan countries for treatments of numerous conditions and diseases of the reproductive system, yet some of the biological activities of lady’s mantle have been poorly studied and neglected. The present study aimed to estimate the potential of A. vulgaris ethanolic extract from Southeast Serbia to prevent and suppress tumor development in vitro, validated by antioxidant, genoprotective, and cytotoxic properties. A total of 45 compounds were detected by UHPLC–HRMS analysis in A. vulgaris ethanolic extract. Measurement of antioxidant activity revealed the significant potential of the tested extract to scavenge free radicals. In addition, the analysis of micronuclei showed an in vitro protective effect on chromosome aberrations in peripheral human lymphocytes. A. vulgaris extract strongly suppressed the growth of human cell lines derived from different types of tumors (MCF-7, A375, A549, and HCT116). The observed antitumor effect is realized through the blockade of cell division, caspase-dependent apoptosis, and autophagic cell death. Our study has shown that Alchemilla vulgaris L. is a valuable source of bioactive compounds able to protect the subcellular structure from damage, thus preventing tumorigenesis as well as suppressing tumor cell growth. © 2022 by the authors.",
journal = "Molecules",
title = "Beyond Traditional Use of Alchemilla vulgaris: Genoprotective and Antitumor Activity In Vitro",
number = "23",
volume = "27",
doi = "10.3390/molecules27238113"
}

Dajić-Stevanović, Z., Jelača, S., Vuković, N., Kolašinac, S., Trendafilova, A., Nedialkov, P., Stanković, M., Tanić, N., Tanić, N. T., Acović, A., Mijatović, S.,& Maksimović-Ivanić, D.. (2022). Beyond Traditional Use of Alchemilla vulgaris: Genoprotective and Antitumor Activity In Vitro. in Molecules, 27(23).
https://doi.org/10.3390/molecules27238113

Dajić-Stevanović Z, Jelača S, Vuković N, Kolašinac S, Trendafilova A, Nedialkov P, Stanković M, Tanić N, Tanić NT, Acović A, Mijatović S, Maksimović-Ivanić D. Beyond Traditional Use of Alchemilla vulgaris: Genoprotective and Antitumor Activity In Vitro. in Molecules. 2022;27(23).
doi:10.3390/molecules27238113 .

Dajić-Stevanović, Zora, Jelača, Sanja, Vuković, Nenad, Kolašinac, Stefan, Trendafilova, Antoaneta, Nedialkov, Paraskev, Stanković, Miroslava, Tanić, Nasta, Tanić, Nikola T., Acović, Aleksandar, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Beyond Traditional Use of Alchemilla vulgaris: Genoprotective and Antitumor Activity In Vitro" in Molecules, 27, no. 23 (2022),
https://doi.org/10.3390/molecules27238113 . .

TY  - JOUR
AU  - Kolašinac, Stefan
AU  - Pećinar, Ilinka
AU  - Danojević, Dario
AU  - Stevanović Dajić, Zora
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6077
AB  - Five Balkan paprika varieties at physiological maturity were investigated by means of Raman spectroscopy in order to discriminate the differences which stemmed from their genetic variability since the plants were grown under the same experimental conditions. The spectra were obtained using the 532 nm wavelength. In an effort to find the best classification power, several pre-processing methods were applied: 1) baseline correction, unit vector normalization; 2) baseline correction, unit vector normalization and first Savitzky-Golay derivative; 3) baseline correction, unit vector normalization and second Savitzky-Golay derivative; 4) baseline correction, unit vector normalization and third Savitzky-Golay derivative. All of the pre-processing methods were followed by making PCA-LDA (Principal Component Analysis-Linear Discriminant Analysis), QDA (Quadratic Discriminant Analysis), and PLS-DA (Partial Least Square - Discriminant Analysis) classification models. QDA showed the best discrimination power (83.87–100% and 89.47–100% for the training and the test data, respectively), then PCA-LDA (0.00–100 and 0.00–100% for the training and the test data, respectively) and PLS-DA (19.35–100% and 0.00–100.00% for the training and the test data, respectively). The results pointed out the applicability of chemometric modeling associated with Raman spectroscopy in the assessment of nutritionally similar samples, such as the studied red paprika varieties.
PB  - Academic Press
T2  - LWT
T1  - Raman spectroscopy coupled with chemometric modeling approaches for authentication of different paprika varieties at physiological maturity
SP  - 113402
VL  - 162
DO  - 10.1016/j.lwt.2022.113402
ER  - 

@article{
author = "Kolašinac, Stefan and Pećinar, Ilinka and Danojević, Dario and Stevanović Dajić, Zora",
year = "2022",
abstract = "Five Balkan paprika varieties at physiological maturity were investigated by means of Raman spectroscopy in order to discriminate the differences which stemmed from their genetic variability since the plants were grown under the same experimental conditions. The spectra were obtained using the 532 nm wavelength. In an effort to find the best classification power, several pre-processing methods were applied: 1) baseline correction, unit vector normalization; 2) baseline correction, unit vector normalization and first Savitzky-Golay derivative; 3) baseline correction, unit vector normalization and second Savitzky-Golay derivative; 4) baseline correction, unit vector normalization and third Savitzky-Golay derivative. All of the pre-processing methods were followed by making PCA-LDA (Principal Component Analysis-Linear Discriminant Analysis), QDA (Quadratic Discriminant Analysis), and PLS-DA (Partial Least Square - Discriminant Analysis) classification models. QDA showed the best discrimination power (83.87–100% and 89.47–100% for the training and the test data, respectively), then PCA-LDA (0.00–100 and 0.00–100% for the training and the test data, respectively) and PLS-DA (19.35–100% and 0.00–100.00% for the training and the test data, respectively). The results pointed out the applicability of chemometric modeling associated with Raman spectroscopy in the assessment of nutritionally similar samples, such as the studied red paprika varieties.",
publisher = "Academic Press",
journal = "LWT",
title = "Raman spectroscopy coupled with chemometric modeling approaches for authentication of different paprika varieties at physiological maturity",
pages = "113402",
volume = "162",
doi = "10.1016/j.lwt.2022.113402"
}

Kolašinac, S., Pećinar, I., Danojević, D.,& Stevanović Dajić, Z.. (2022). Raman spectroscopy coupled with chemometric modeling approaches for authentication of different paprika varieties at physiological maturity. in LWT
Academic Press., 162, 113402.
https://doi.org/10.1016/j.lwt.2022.113402

Kolašinac S, Pećinar I, Danojević D, Stevanović Dajić Z. Raman spectroscopy coupled with chemometric modeling approaches for authentication of different paprika varieties at physiological maturity. in LWT. 2022;162:113402.
doi:10.1016/j.lwt.2022.113402 .

Kolašinac, Stefan, Pećinar, Ilinka, Danojević, Dario, Stevanović Dajić, Zora, "Raman spectroscopy coupled with chemometric modeling approaches for authentication of different paprika varieties at physiological maturity" in LWT, 162 (2022):113402,
https://doi.org/10.1016/j.lwt.2022.113402 . .

TY  - CONF
AU  - Kolašinac, Stefan
AU  - Kostić, Aleksandar Ž.
AU  - Maksimović, Danijela
AU  - Mijatović, Sanja
AU  - Tanić, Nikola
AU  - Nebojša, Arsenijević
AU  - Zora P., Dajić Stevanović
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6640
AB  - Viscum album L. (Loranthaceae Juss.) is semi-parasitic evergreen shrub distributed in Europe, northwestern part of Africa and
Anatolia. The species is hosted by different woody gymnosperm and angiosperm species and it is known that host preference usually reflects in differences in morphological traits and possibly in phytochemical composition. Therefore, there are
different subspecies, out of three subspecies (V. album subsp. abietis (Wiesb.) Janchen, subsp. creticum N. Böhling & al. and subsp. austriacum (Wiesb.) Vollm.) occur most frequently on conifers while subsp. album is confined to deciduous host trees
and shrubs. In addition to religious and mystical uses, the mistletoe is used in the folk medicine for circulatory and respiratory
disorders and as an anticancer remedy.
PB  - Novi Sad : Prirodno-matematički fakultet
C3  - The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC
T1  - T4-P-14 Mistletoe (Viscum Album L.) as a source of valuable antioxidants
EP  - 225
SP  - 224
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6640
ER  - 

@conference{
author = "Kolašinac, Stefan and Kostić, Aleksandar Ž. and Maksimović, Danijela and Mijatović, Sanja and Tanić, Nikola and Nebojša, Arsenijević and Zora P., Dajić Stevanović",
year = "2021",
abstract = "Viscum album L. (Loranthaceae Juss.) is semi-parasitic evergreen shrub distributed in Europe, northwestern part of Africa and
Anatolia. The species is hosted by different woody gymnosperm and angiosperm species and it is known that host preference usually reflects in differences in morphological traits and possibly in phytochemical composition. Therefore, there are
different subspecies, out of three subspecies (V. album subsp. abietis (Wiesb.) Janchen, subsp. creticum N. Böhling & al. and subsp. austriacum (Wiesb.) Vollm.) occur most frequently on conifers while subsp. album is confined to deciduous host trees
and shrubs. In addition to religious and mystical uses, the mistletoe is used in the folk medicine for circulatory and respiratory
disorders and as an anticancer remedy.",
publisher = "Novi Sad : Prirodno-matematički fakultet",
journal = "The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC",
title = "T4-P-14 Mistletoe (Viscum Album L.) as a source of valuable antioxidants",
pages = "225-224",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6640"
}

Kolašinac, S., Kostić, A. Ž., Maksimović, D., Mijatović, S., Tanić, N., Nebojša, A.,& Zora P., D. S.. (2021). T4-P-14 Mistletoe (Viscum Album L.) as a source of valuable antioxidants. in The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC
Novi Sad : Prirodno-matematički fakultet., 224-225.
https://hdl.handle.net/21.15107/rcub_agrospace_6640

Kolašinac S, Kostić AŽ, Maksimović D, Mijatović S, Tanić N, Nebojša A, Zora P. DS. T4-P-14 Mistletoe (Viscum Album L.) as a source of valuable antioxidants. in The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC. 2021;:224-225.
https://hdl.handle.net/21.15107/rcub_agrospace_6640 .

Kolašinac, Stefan, Kostić, Aleksandar Ž., Maksimović, Danijela, Mijatović, Sanja, Tanić, Nikola, Nebojša, Arsenijević, Zora P., Dajić Stevanović, "T4-P-14 Mistletoe (Viscum Album L.) as a source of valuable antioxidants" in The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC (2021):224-225,
https://hdl.handle.net/21.15107/rcub_agrospace_6640 .

TY  - JOUR
AU  - Kolašinac, Stefan M.
AU  - Dajić-Stevanović, Zora
AU  - Kilibarda, Sofija N.
AU  - Kostić, Aleksandar Ž.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5861
AB  - Carotenoids represent a large group of mainly red, orange, and yellow natural metabolites mainly involved in regulation of many metabolic processes. Carotenoids are beneficial for human health. Current study describes the importance, chemical composition and functioning of carotenoids. It is well known that carotenoids support pigments acting in light absorbance mechanisms during photosynthesis, and are known to protect the chlorophyll molecules from oxidative stress and reactive oxygen species (ROS) damage. Carotenoids are involved in signaling processes in plants, responses to environmental stresses, pollination, germination and reproduction, and development regulation. As nutrients of strong antioxidant activity that is primarily linked to their polyene molecular structure, the carotenoids are reported as immune-enhancement and anticancer agents, which are also involved in prevention of eye-, gastric and neurocognitive disorders, and in regulation of obesity and anti-ageing. Concern-ing the wide prospective applications of carotenoids as pharmaceuticals and nutraceuticals, there are some critical aspects associated with carotenoids’ bioavailability and challenges in their bioengineering. This mostly refers to the needs for identification and cloning of genes responsible for carotenoid biosynthesis and transformation and related development of transgenic carotenoid-rich crops. In the recent years, technologies of micro-and nanoencapsulation have addressed the needs of carotenoid entrapping to enhance their bioavailability, solubility and chemical stability, and to ensure the target delivery and manifestation of their strong antioxidant and other biological activity. Among standard and some advanced analytic tools for carotenoid determination (e.g., High performance liquid chromatography-HPLC, Liquid chromatography–mass spectrometry-LC-MS, Ultra high performance liquid chromatography-UHPLC, High-performance thin-layer chromatography-HPTLC and others), the vibrational spectroscopy techniques, primarily Raman spectroscopy coupled with chemometric modeling, opened a new era in carotenoid research and application.
PB  - Tech Science Press
T2  - Phyton
T1  - Carotenoids: New Applications of “Old” Pigments
EP  - 1062
IS  - 4
SP  - 1041
VL  - 90
DO  - 10.32604/phyton.2021.015996
ER  - 

@article{
author = "Kolašinac, Stefan M. and Dajić-Stevanović, Zora and Kilibarda, Sofija N. and Kostić, Aleksandar Ž.",
year = "2021",
abstract = "Carotenoids represent a large group of mainly red, orange, and yellow natural metabolites mainly involved in regulation of many metabolic processes. Carotenoids are beneficial for human health. Current study describes the importance, chemical composition and functioning of carotenoids. It is well known that carotenoids support pigments acting in light absorbance mechanisms during photosynthesis, and are known to protect the chlorophyll molecules from oxidative stress and reactive oxygen species (ROS) damage. Carotenoids are involved in signaling processes in plants, responses to environmental stresses, pollination, germination and reproduction, and development regulation. As nutrients of strong antioxidant activity that is primarily linked to their polyene molecular structure, the carotenoids are reported as immune-enhancement and anticancer agents, which are also involved in prevention of eye-, gastric and neurocognitive disorders, and in regulation of obesity and anti-ageing. Concern-ing the wide prospective applications of carotenoids as pharmaceuticals and nutraceuticals, there are some critical aspects associated with carotenoids’ bioavailability and challenges in their bioengineering. This mostly refers to the needs for identification and cloning of genes responsible for carotenoid biosynthesis and transformation and related development of transgenic carotenoid-rich crops. In the recent years, technologies of micro-and nanoencapsulation have addressed the needs of carotenoid entrapping to enhance their bioavailability, solubility and chemical stability, and to ensure the target delivery and manifestation of their strong antioxidant and other biological activity. Among standard and some advanced analytic tools for carotenoid determination (e.g., High performance liquid chromatography-HPLC, Liquid chromatography–mass spectrometry-LC-MS, Ultra high performance liquid chromatography-UHPLC, High-performance thin-layer chromatography-HPTLC and others), the vibrational spectroscopy techniques, primarily Raman spectroscopy coupled with chemometric modeling, opened a new era in carotenoid research and application.",
publisher = "Tech Science Press",
journal = "Phyton",
title = "Carotenoids: New Applications of “Old” Pigments",
pages = "1062-1041",
number = "4",
volume = "90",
doi = "10.32604/phyton.2021.015996"
}

Kolašinac, S. M., Dajić-Stevanović, Z., Kilibarda, S. N.,& Kostić, A. Ž.. (2021). Carotenoids: New Applications of “Old” Pigments. in Phyton
Tech Science Press., 90(4), 1041-1062.
https://doi.org/10.32604/phyton.2021.015996

Kolašinac SM, Dajić-Stevanović Z, Kilibarda SN, Kostić AŽ. Carotenoids: New Applications of “Old” Pigments. in Phyton. 2021;90(4):1041-1062.
doi:10.32604/phyton.2021.015996 .

Kolašinac, Stefan M., Dajić-Stevanović, Zora, Kilibarda, Sofija N., Kostić, Aleksandar Ž., "Carotenoids: New Applications of “Old” Pigments" in Phyton, 90, no. 4 (2021):1041-1062,
https://doi.org/10.32604/phyton.2021.015996 . .

TY  - CONF
AU  - Kolašinac, Stefan M.
AU  - Pećinar, Ilinka
AU  - Dajić Stevanović, Zora P.
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6549
AB  - INTRODUCTION:
According to Codex Alimentarius (2001), “Honey is the natural sweet substance, produced by honeybees from the nectar of
plants or from secretions of living parts of plants, or excretions of plant-sucking insects on the living parts of plants, which
the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in honeycombs to ripen and mature”. Honey is mostly made up of sugars, as well as enzymes, amino acids, organic acids, vitamins,
aromatic compounds, minerals and carotenoids. It contains a lot of flavonoids and phenolic acids, which have a lot of biological effects and functions such as natural antioxidants, anti-inflammatory and antimicrobial properties. Its composition
is particularly variable, depending on its botanical and geographical origins Because of its exclusive flavor and high dietary
value, natural honey is more expensive than other sweeteners. This is the reason why honey is a target of adulteration. The
problem is that counterfeiting honey is relatively easy, but detection is difficult. Further, the authenticity of honey is a global
important problem for commercial producers and consumers. Accordingly, a fast and non-destructive method of detecting
counterfeits is needed. 
OBJECTIVES:
The aim of this paper is to verify the possibility of Raman spectroscopy and Support Vector Machine (SVM) for classification
of two different honeys and their fake duplicates. For this purpose, meadow and acacia honeys were selected.
METHOD / DESIGN:
Spectra of homemade and counterfeits honey were recorded using XploRA Raman spectrometer (Horiba Jobin Yvon). Raman scattering was excited by laser at a wavelength of 785 nm equipped with a 600 lines/mm grating; spectra were recorded
by applying exposure time 10 s and accumulated from 10 times scans, using 100% filter. Spectral resolution was 3 cm−1 and
autocalibration was done each time before recording of spectra by 520.47 cm−1 line of silicon. In order to assess a possible
sample inhomogeneity, thirty Raman spectra in the region from 200-3400 cm-1 were recorded for each sample. All spectra
were baseline-corrected, normalized and smoothed. After that PCA (Principal component analysis) was conducted and obtained PCs (first two PCs) served as a features for support vector machine (SVM) classification method. Data were divided into
training model (70 %) and training data (30 %). Pre-processing was done by Unscrambler X 10.4 software (CAMO software,
Norway). In order to determine the best shape of the hyperplane and decision boundary, several kernel function were used:
linear, radial basis and polynomial function. The SVM was conducted by Python and Scikit-learn package.
RESULTS:
Support vector machine showed high accuracy in classification of different honey samples. Accordingly, the best discrimination power showed SVM with polynomial function (100%), followed by radial basis (96.67%) and linear (81.82%).
T1  - Machine learning chemometric model for Raman spectroscopy based honey quality assessment
EP  - 5
SP  - 5
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6549
ER  - 

@conference{
author = "Kolašinac, Stefan M. and Pećinar, Ilinka and Dajić Stevanović, Zora P.",
year = "2021",
abstract = "INTRODUCTION:
According to Codex Alimentarius (2001), “Honey is the natural sweet substance, produced by honeybees from the nectar of
plants or from secretions of living parts of plants, or excretions of plant-sucking insects on the living parts of plants, which
the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in honeycombs to ripen and mature”. Honey is mostly made up of sugars, as well as enzymes, amino acids, organic acids, vitamins,
aromatic compounds, minerals and carotenoids. It contains a lot of flavonoids and phenolic acids, which have a lot of biological effects and functions such as natural antioxidants, anti-inflammatory and antimicrobial properties. Its composition
is particularly variable, depending on its botanical and geographical origins Because of its exclusive flavor and high dietary
value, natural honey is more expensive than other sweeteners. This is the reason why honey is a target of adulteration. The
problem is that counterfeiting honey is relatively easy, but detection is difficult. Further, the authenticity of honey is a global
important problem for commercial producers and consumers. Accordingly, a fast and non-destructive method of detecting
counterfeits is needed. 
OBJECTIVES:
The aim of this paper is to verify the possibility of Raman spectroscopy and Support Vector Machine (SVM) for classification
of two different honeys and their fake duplicates. For this purpose, meadow and acacia honeys were selected.
METHOD / DESIGN:
Spectra of homemade and counterfeits honey were recorded using XploRA Raman spectrometer (Horiba Jobin Yvon). Raman scattering was excited by laser at a wavelength of 785 nm equipped with a 600 lines/mm grating; spectra were recorded
by applying exposure time 10 s and accumulated from 10 times scans, using 100% filter. Spectral resolution was 3 cm−1 and
autocalibration was done each time before recording of spectra by 520.47 cm−1 line of silicon. In order to assess a possible
sample inhomogeneity, thirty Raman spectra in the region from 200-3400 cm-1 were recorded for each sample. All spectra
were baseline-corrected, normalized and smoothed. After that PCA (Principal component analysis) was conducted and obtained PCs (first two PCs) served as a features for support vector machine (SVM) classification method. Data were divided into
training model (70 %) and training data (30 %). Pre-processing was done by Unscrambler X 10.4 software (CAMO software,
Norway). In order to determine the best shape of the hyperplane and decision boundary, several kernel function were used:
linear, radial basis and polynomial function. The SVM was conducted by Python and Scikit-learn package.
RESULTS:
Support vector machine showed high accuracy in classification of different honey samples. Accordingly, the best discrimination power showed SVM with polynomial function (100%), followed by radial basis (96.67%) and linear (81.82%).",
title = "Machine learning chemometric model for Raman spectroscopy based honey quality assessment",
pages = "5-5",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6549"
}

Kolašinac, S. M., Pećinar, I.,& Dajić Stevanović, Z. P.. (2021). Machine learning chemometric model for Raman spectroscopy based honey quality assessment. , 5-5.
https://hdl.handle.net/21.15107/rcub_agrospace_6549

Kolašinac SM, Pećinar I, Dajić Stevanović ZP. Machine learning chemometric model for Raman spectroscopy based honey quality assessment. 2021;:5-5.
https://hdl.handle.net/21.15107/rcub_agrospace_6549 .

Kolašinac, Stefan M., Pećinar, Ilinka, Dajić Stevanović, Zora P., "Machine learning chemometric model for Raman spectroscopy based honey quality assessment" (2021):5-5,
https://hdl.handle.net/21.15107/rcub_agrospace_6549 .

TY  - JOUR
AU  - Kolašinac, Stefan
AU  - Pećinar, Ilinka
AU  - Danojević, Dario
AU  - Aćić, Svetlana
AU  - Dajić-Stevanović, Zora
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5932
AB  - The main goal of the present study is validation of different chemometric models in Raman spectroscopic monitoring of different maturity phases of the red pepper fruit. Successive ripening stages commonly corresponding with different fruit coloration (green, green-brown, brown-red, and deep red) were assessed. The fruit maturation process in red pepper is related to alteration in composition and content of different primary and secondary metabolites, including the most represented carotenoid, the capsanthin. Raman microspectroscopy with wavelength of 532 nm was used to obtain the spectra of the pericarp of different maturation phases. Several multivariate classification methods, such as Principal Component Analysis–Linear Discriminant Analysis (PCA–LDA), Partial Least Squares-Discriminant Analysis (PLS-DA), and Soft Independent Modeling of Class Analogy (SIMCA) were tested to determine the model which best fits the target ripening phases related to composition and the content of the most represented carotenoids. Concerning different fruit ripening phases, several characteristic bands were obtained, including those at 1514–1517, 1151, and 1003 cm−1, all assigned to carotenoids with nine conjugated double bonds in the main polyene chain (e.g., lutein, beta carotene, beta cryptoxanthin, zeaxanthin, capsorubin, and capsanthin). All tested classification chemometric models had a high rate of prediction accuracy (between 95% and 100%). The SIMCA showed the best result, probably because of using a different algorithm compared with the other tested models.
PB  - John Wiley and Sons Ltd
T2  - Journal of Raman Spectroscopy
T1  - Raman spectroscopic-based chemometric modeling inassessment of red pepper ripening phases and carotenoidsaccumulation
EP  - 1605
IS  - 9
SP  - 1598
VL  - 52
DO  - 10.1002/jrs.6197
ER  - 

@article{
author = "Kolašinac, Stefan and Pećinar, Ilinka and Danojević, Dario and Aćić, Svetlana and Dajić-Stevanović, Zora",
year = "2021",
abstract = "The main goal of the present study is validation of different chemometric models in Raman spectroscopic monitoring of different maturity phases of the red pepper fruit. Successive ripening stages commonly corresponding with different fruit coloration (green, green-brown, brown-red, and deep red) were assessed. The fruit maturation process in red pepper is related to alteration in composition and content of different primary and secondary metabolites, including the most represented carotenoid, the capsanthin. Raman microspectroscopy with wavelength of 532 nm was used to obtain the spectra of the pericarp of different maturation phases. Several multivariate classification methods, such as Principal Component Analysis–Linear Discriminant Analysis (PCA–LDA), Partial Least Squares-Discriminant Analysis (PLS-DA), and Soft Independent Modeling of Class Analogy (SIMCA) were tested to determine the model which best fits the target ripening phases related to composition and the content of the most represented carotenoids. Concerning different fruit ripening phases, several characteristic bands were obtained, including those at 1514–1517, 1151, and 1003 cm−1, all assigned to carotenoids with nine conjugated double bonds in the main polyene chain (e.g., lutein, beta carotene, beta cryptoxanthin, zeaxanthin, capsorubin, and capsanthin). All tested classification chemometric models had a high rate of prediction accuracy (between 95% and 100%). The SIMCA showed the best result, probably because of using a different algorithm compared with the other tested models.",
publisher = "John Wiley and Sons Ltd",
journal = "Journal of Raman Spectroscopy",
title = "Raman spectroscopic-based chemometric modeling inassessment of red pepper ripening phases and carotenoidsaccumulation",
pages = "1605-1598",
number = "9",
volume = "52",
doi = "10.1002/jrs.6197"
}

Kolašinac, S., Pećinar, I., Danojević, D., Aćić, S.,& Dajić-Stevanović, Z.. (2021). Raman spectroscopic-based chemometric modeling inassessment of red pepper ripening phases and carotenoidsaccumulation. in Journal of Raman Spectroscopy
John Wiley and Sons Ltd., 52(9), 1598-1605.
https://doi.org/10.1002/jrs.6197

Kolašinac S, Pećinar I, Danojević D, Aćić S, Dajić-Stevanović Z. Raman spectroscopic-based chemometric modeling inassessment of red pepper ripening phases and carotenoidsaccumulation. in Journal of Raman Spectroscopy. 2021;52(9):1598-1605.
doi:10.1002/jrs.6197 .

Kolašinac, Stefan, Pećinar, Ilinka, Danojević, Dario, Aćić, Svetlana, Dajić-Stevanović, Zora, "Raman spectroscopic-based chemometric modeling inassessment of red pepper ripening phases and carotenoidsaccumulation" in Journal of Raman Spectroscopy, 52, no. 9 (2021):1598-1605,
https://doi.org/10.1002/jrs.6197 . .

TY  - JOUR
AU  - Miladinović, Vladimir
AU  - Kolašinac, Stefan
AU  - Pećinar, Ilinka
AU  - Kiprovski, Biljana
AU  - Mutavdžić, Dragosav
AU  - Ugrenović, Vladan
AU  - Dajić-Stevanović, Zora
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5937
AB  - Effects of genotype and Bradyrhizobium inoculation on morphological traits, grain yield and protein content of soybean varieties.- Genetika, Vol 53, No.2, 911-925. Soybean crop production in Serbia involves seed inoculation by N-fixing bacteria just before sowing time. The main objective of the current work was to assess the impact of the genotype and inoculation on range of morphological and yield traits of soybean (Glycine max L. Merrill), as well as the total protein content. The experiment was conducted on chernozem soil, where soybean was previously grown. The six local varieties were used, where each variety was sown, in three replicates for both inoculated and non-inoculated treatment. The following morphological traits were analysed: the plant height, number of lateral branches, distance to the first pod, number of pods per plant, pods (containing seeds) weight per plant, seed weight per plant, and the total grain yield. The total protein content in seeds was determined by standard analytical method, while subtle differences in qualitative protein composition were assessed using Raman spectroscopy. The total protein content varied from 39.6 to 42.15 %. Performance of inoculation resulted in an increase of the plant height and the distance to the first pod, although not in all tested varieties. The highest and the lowest plant height values were observed for non-inoculated variety Dana (59.23cm) and Sava (80.03cm), respectively. The effect of genotype was much more expressed causing differences in almost all tested characters, except for the total protein content. However, Raman spectroscopy analyses revealed distinct discrimination among surveyed varieties, and differences between inoculated and non-inoculated plants in qualitative composition of seed proteins.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Effects Of Genotype And Bradyrhizobium Inoculation On Morphological Traits, Grain Yield And Protein Content Of Soybean Varieties
EP  - 925
IS  - 2
SP  - 911
VL  - 53
DO  - 10.2298/GENSR2102911M
ER  - 

@article{
author = "Miladinović, Vladimir and Kolašinac, Stefan and Pećinar, Ilinka and Kiprovski, Biljana and Mutavdžić, Dragosav and Ugrenović, Vladan and Dajić-Stevanović, Zora",
year = "2021",
abstract = "Effects of genotype and Bradyrhizobium inoculation on morphological traits, grain yield and protein content of soybean varieties.- Genetika, Vol 53, No.2, 911-925. Soybean crop production in Serbia involves seed inoculation by N-fixing bacteria just before sowing time. The main objective of the current work was to assess the impact of the genotype and inoculation on range of morphological and yield traits of soybean (Glycine max L. Merrill), as well as the total protein content. The experiment was conducted on chernozem soil, where soybean was previously grown. The six local varieties were used, where each variety was sown, in three replicates for both inoculated and non-inoculated treatment. The following morphological traits were analysed: the plant height, number of lateral branches, distance to the first pod, number of pods per plant, pods (containing seeds) weight per plant, seed weight per plant, and the total grain yield. The total protein content in seeds was determined by standard analytical method, while subtle differences in qualitative protein composition were assessed using Raman spectroscopy. The total protein content varied from 39.6 to 42.15 %. Performance of inoculation resulted in an increase of the plant height and the distance to the first pod, although not in all tested varieties. The highest and the lowest plant height values were observed for non-inoculated variety Dana (59.23cm) and Sava (80.03cm), respectively. The effect of genotype was much more expressed causing differences in almost all tested characters, except for the total protein content. However, Raman spectroscopy analyses revealed distinct discrimination among surveyed varieties, and differences between inoculated and non-inoculated plants in qualitative composition of seed proteins.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Effects Of Genotype And Bradyrhizobium Inoculation On Morphological Traits, Grain Yield And Protein Content Of Soybean Varieties",
pages = "925-911",
number = "2",
volume = "53",
doi = "10.2298/GENSR2102911M"
}

Miladinović, V., Kolašinac, S., Pećinar, I., Kiprovski, B., Mutavdžić, D., Ugrenović, V.,& Dajić-Stevanović, Z.. (2021). Effects Of Genotype And Bradyrhizobium Inoculation On Morphological Traits, Grain Yield And Protein Content Of Soybean Varieties. in Genetika
Serbian Genetics Society., 53(2), 911-925.
https://doi.org/10.2298/GENSR2102911M

Miladinović V, Kolašinac S, Pećinar I, Kiprovski B, Mutavdžić D, Ugrenović V, Dajić-Stevanović Z. Effects Of Genotype And Bradyrhizobium Inoculation On Morphological Traits, Grain Yield And Protein Content Of Soybean Varieties. in Genetika. 2021;53(2):911-925.
doi:10.2298/GENSR2102911M .

Miladinović, Vladimir, Kolašinac, Stefan, Pećinar, Ilinka, Kiprovski, Biljana, Mutavdžić, Dragosav, Ugrenović, Vladan, Dajić-Stevanović, Zora, "Effects Of Genotype And Bradyrhizobium Inoculation On Morphological Traits, Grain Yield And Protein Content Of Soybean Varieties" in Genetika, 53, no. 2 (2021):911-925,
https://doi.org/10.2298/GENSR2102911M . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Janacković, Pedja
AU  - Kolasinac, Stefan M.
AU  - Dajić-Stevanović, Zora
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5763
AB  - Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry
IS  - 6
VL  - 17
DO  - 10.1002/cbdv.202000097
ER  - 

@article{
author = "Kostić, Aleksandar and Janacković, Pedja and Kolasinac, Stefan M. and Dajić-Stevanović, Zora",
year = "2020",
abstract = "Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry",
number = "6",
volume = "17",
doi = "10.1002/cbdv.202000097"
}

Kostić, A., Janacković, P., Kolasinac, S. M.,& Dajić-Stevanović, Z.. (2020). Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 17(6).
https://doi.org/10.1002/cbdv.202000097

Kostić A, Janacković P, Kolasinac SM, Dajić-Stevanović Z. Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity. 2020;17(6).
doi:10.1002/cbdv.202000097 .

Kostić, Aleksandar, Janacković, Pedja, Kolasinac, Stefan M., Dajić-Stevanović, Zora, "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry" in Chemistry & Biodiversity, 17, no. 6 (2020),
https://doi.org/10.1002/cbdv.202000097 . .

TY  - JOUR
AU  - Mladenović, Marko
AU  - Grčić, Nikola
AU  - Stevanović, Milan
AU  - Đorđević Melnik, Olivera
AU  - Nikolić, Milica
AU  - Kolašinac, Stefan
AU  - Prodanović, Slaven
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6509
AB  - The grain yield and its components of eighteen experimental, one registered and two
commercial maize hybrids were examined in six different environments. The main objectives
were to identify the best performing experimental and registered hybrids and to select
appropriate hybrids for approving and commercialization. Based on the results, many decisions
are made. Experimental hybrid Exp.15 is submitted for testing to the Variety Commission of
the Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia. This
hybrid, as a high-yielding and a stable one, could enrich the assortment of registered mediumlate maize hybrids in Serbia. First year of its testing for the purpose of being registered is going
to be 2020. Further, registered hybrid ZP 685 is suggested for commercialization as one of the
best performing in present study. In addition, the goal of this study was also identification of
the superior inbred lines for use in future breeding programs. Inbred lines L1 (BSSS) and L4
(Independent heterotic group) are chosen for crossing with an aim to create a new origin
population - source for deriving new recombinant inbred lines. Cross L1 x L4 will be a part of
maize breeding program at Maize Research Institute “Zemun Polje” in 2020. Lines L1 and L4
are also suggested for crossing with all others Lancaster lines from MRIZP (which are not
parental components of tested hybrids) for deriving new experimental F1 hybrids. As well,
Lancaster lines L5, L6 and L7 should be crossed between themselves for creating new origin
populations for deriving new recombinant Lancaster inbred lines. Hybrid Sister 1 had very high
and stable grain yield and as such it is the most cost effective for seed production.
PB  - University of Banja Luka, Faculty of Agriculture
T2  - IX International Symposium on Agricultural Sciences "AgroReS 2020"
T1  - Evaluation of grain yield and its components of some experimental registreted and commercial ZP maize (Zea mays L.) hybrids
EP  - 37
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6509
ER  - 

@article{
author = "Mladenović, Marko and Grčić, Nikola and Stevanović, Milan and Đorđević Melnik, Olivera and Nikolić, Milica and Kolašinac, Stefan and Prodanović, Slaven",
year = "2020",
abstract = "The grain yield and its components of eighteen experimental, one registered and two
commercial maize hybrids were examined in six different environments. The main objectives
were to identify the best performing experimental and registered hybrids and to select
appropriate hybrids for approving and commercialization. Based on the results, many decisions
are made. Experimental hybrid Exp.15 is submitted for testing to the Variety Commission of
the Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia. This
hybrid, as a high-yielding and a stable one, could enrich the assortment of registered mediumlate maize hybrids in Serbia. First year of its testing for the purpose of being registered is going
to be 2020. Further, registered hybrid ZP 685 is suggested for commercialization as one of the
best performing in present study. In addition, the goal of this study was also identification of
the superior inbred lines for use in future breeding programs. Inbred lines L1 (BSSS) and L4
(Independent heterotic group) are chosen for crossing with an aim to create a new origin
population - source for deriving new recombinant inbred lines. Cross L1 x L4 will be a part of
maize breeding program at Maize Research Institute “Zemun Polje” in 2020. Lines L1 and L4
are also suggested for crossing with all others Lancaster lines from MRIZP (which are not
parental components of tested hybrids) for deriving new experimental F1 hybrids. As well,
Lancaster lines L5, L6 and L7 should be crossed between themselves for creating new origin
populations for deriving new recombinant Lancaster inbred lines. Hybrid Sister 1 had very high
and stable grain yield and as such it is the most cost effective for seed production.",
publisher = "University of Banja Luka, Faculty of Agriculture",
journal = "IX International Symposium on Agricultural Sciences "AgroReS 2020"",
title = "Evaluation of grain yield and its components of some experimental registreted and commercial ZP maize (Zea mays L.) hybrids",
pages = "37-25",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6509"
}

Mladenović, M., Grčić, N., Stevanović, M., Đorđević Melnik, O., Nikolić, M., Kolašinac, S.,& Prodanović, S.. (2020). Evaluation of grain yield and its components of some experimental registreted and commercial ZP maize (Zea mays L.) hybrids. in IX International Symposium on Agricultural Sciences "AgroReS 2020"
University of Banja Luka, Faculty of Agriculture., 25-37.
https://hdl.handle.net/21.15107/rcub_agrospace_6509

Mladenović M, Grčić N, Stevanović M, Đorđević Melnik O, Nikolić M, Kolašinac S, Prodanović S. Evaluation of grain yield and its components of some experimental registreted and commercial ZP maize (Zea mays L.) hybrids. in IX International Symposium on Agricultural Sciences "AgroReS 2020". 2020;:25-37.
https://hdl.handle.net/21.15107/rcub_agrospace_6509 .

Mladenović, Marko, Grčić, Nikola, Stevanović, Milan, Đorđević Melnik, Olivera, Nikolić, Milica, Kolašinac, Stefan, Prodanović, Slaven, "Evaluation of grain yield and its components of some experimental registreted and commercial ZP maize (Zea mays L.) hybrids" in IX International Symposium on Agricultural Sciences "AgroReS 2020" (2020):25-37,
https://hdl.handle.net/21.15107/rcub_agrospace_6509 .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Janacković, Pedja
AU  - Kolasinac, Stefan M.
AU  - Dajić-Stevanović, Zora
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5399
AB  - Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry
IS  - 6
VL  - 17
DO  - 10.1002/cbdv.202000097
ER  - 

@article{
author = "Kostić, Aleksandar and Janacković, Pedja and Kolasinac, Stefan M. and Dajić-Stevanović, Zora",
year = "2020",
abstract = "Herbal drugs are a useful source of different bioactive compounds. Asteraceae species, as the most widespread vascular plants, can be used both as food and as medicine due to the great diversity of recorded chemical components - different phenolic compounds, terpenes, carotenoids, vitamins, alkaloids, etc. The Balkan Peninsula is characterized by great diversity of plants from Asteraceae family, including presence of rare and endemic species. In this review, results of the survey of chemical composition and biological activity, mainly focusing on antioxidant, antimicrobial and anticancer effects of selected Balkans' Asteraceae species were provided. In addition, information on edible plants from Asteraceae family is presented, due to growing interest for the so-called 'healthy diet' and possible application of Balkans' Asteraceae species as food of high nutritional value or as a source of functional food ingredients.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry",
number = "6",
volume = "17",
doi = "10.1002/cbdv.202000097"
}

Kostić, A., Janacković, P., Kolasinac, S. M.,& Dajić-Stevanović, Z.. (2020). Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 17(6).
https://doi.org/10.1002/cbdv.202000097

Kostić A, Janacković P, Kolasinac SM, Dajić-Stevanović Z. Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry. in Chemistry & Biodiversity. 2020;17(6).
doi:10.1002/cbdv.202000097 .

Kostić, Aleksandar, Janacković, Pedja, Kolasinac, Stefan M., Dajić-Stevanović, Zora, "Balkans' Asteraceae Species as a Source of Biologically Active Compounds for the Pharmaceutical and Food Industry" in Chemistry & Biodiversity, 17, no. 6 (2020),
https://doi.org/10.1002/cbdv.202000097 . .

TY  - JOUR
AU  - Kolasinac, Stefan M.
AU  - Bosnić, Predrag
AU  - Lekić, Slavoljub
AU  - Golijan, Jelena
AU  - Petrović, Tanja
AU  - Todorović, Goran
AU  - Kostić, Aleksandar
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4671
AB  - The original version of this article unfortunately contained a mistake in the author group, affiliation and funding information sections as well as the omitted acknowledgment section.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment (vol 190, 508, 2018)
IS  - 11
VL  - 190
DO  - 10.1007/s10661-018-7047-y
ER  - 

@article{
author = "Kolasinac, Stefan M. and Bosnić, Predrag and Lekić, Slavoljub and Golijan, Jelena and Petrović, Tanja and Todorović, Goran and Kostić, Aleksandar",
year = "2018",
abstract = "The original version of this article unfortunately contained a mistake in the author group, affiliation and funding information sections as well as the omitted acknowledgment section.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment (vol 190, 508, 2018)",
number = "11",
volume = "190",
doi = "10.1007/s10661-018-7047-y"
}

Kolasinac, S. M., Bosnić, P., Lekić, S., Golijan, J., Petrović, T., Todorović, G.,& Kostić, A.. (2018). Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment (vol 190, 508, 2018). in Environmental Monitoring and Assessment
Springer, Dordrecht., 190(11).
https://doi.org/10.1007/s10661-018-7047-y

Kolasinac SM, Bosnić P, Lekić S, Golijan J, Petrović T, Todorović G, Kostić A. Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment (vol 190, 508, 2018). in Environmental Monitoring and Assessment. 2018;190(11).
doi:10.1007/s10661-018-7047-y .

Kolasinac, Stefan M., Bosnić, Predrag, Lekić, Slavoljub, Golijan, Jelena, Petrović, Tanja, Todorović, Goran, Kostić, Aleksandar, "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment (vol 190, 508, 2018)" in Environmental Monitoring and Assessment, 190, no. 11 (2018),
https://doi.org/10.1007/s10661-018-7047-y . .

TY  - JOUR
AU  - Kolasinac, Stefan M.
AU  - Lekić, Slavoljub
AU  - Golijan, Jelena
AU  - Petrović, Tanja
AU  - Todorović, Goran
AU  - Kostić, Aleksandar
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4653
AB  - The aim of this work was to determine elements composition and bioaccumulation process in ripe tomato fruits influenced by zinc feeding of plants which was applied in three different doses. Macro- and microelement content in growing soil, seeds, and fruits was determined by ICP-OES method. Health risk assessment was calculated according to the presence of some toxic elements. It was found that predominant macroelements were phosphorus, potassium, calcium, and magnesium followed by other ten determined elements. The presence of five potentially toxic elements (cadmium, chromium, lead, nickel, and strontium) in seed and fruits was detected. Bioaccumulation differences (especially in case of potassium) for some elements in seed and fruit were established. In both cases, calcium and lead were the only elements with antagonistic effect towards zinc feeding process. Health risk assessment has shown that acute risk is low for all toxic elements (according to acute hazard quotient (HQ) calculation) except for cadmium in fruit seed, where it can be characterized as moderate. Long-term hazard quotient calculation showed moderate risk in the case of lead (fruit skin and seed) and low values for other toxic elements. Since the most part of toxic elements was accumulated in tomato fruit skin and seed, peeling of fruits can significantly reduce health risk.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment
IS  - 9
VL  - 190
DO  - 10.1007/s10661-018-6886-x
ER  - 

@article{
author = "Kolasinac, Stefan M. and Lekić, Slavoljub and Golijan, Jelena and Petrović, Tanja and Todorović, Goran and Kostić, Aleksandar",
year = "2018",
abstract = "The aim of this work was to determine elements composition and bioaccumulation process in ripe tomato fruits influenced by zinc feeding of plants which was applied in three different doses. Macro- and microelement content in growing soil, seeds, and fruits was determined by ICP-OES method. Health risk assessment was calculated according to the presence of some toxic elements. It was found that predominant macroelements were phosphorus, potassium, calcium, and magnesium followed by other ten determined elements. The presence of five potentially toxic elements (cadmium, chromium, lead, nickel, and strontium) in seed and fruits was detected. Bioaccumulation differences (especially in case of potassium) for some elements in seed and fruit were established. In both cases, calcium and lead were the only elements with antagonistic effect towards zinc feeding process. Health risk assessment has shown that acute risk is low for all toxic elements (according to acute hazard quotient (HQ) calculation) except for cadmium in fruit seed, where it can be characterized as moderate. Long-term hazard quotient calculation showed moderate risk in the case of lead (fruit skin and seed) and low values for other toxic elements. Since the most part of toxic elements was accumulated in tomato fruit skin and seed, peeling of fruits can significantly reduce health risk.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment",
number = "9",
volume = "190",
doi = "10.1007/s10661-018-6886-x"
}

Kolasinac, S. M., Lekić, S., Golijan, J., Petrović, T., Todorović, G.,& Kostić, A.. (2018). Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment. in Environmental Monitoring and Assessment
Springer, Dordrecht., 190(9).
https://doi.org/10.1007/s10661-018-6886-x

Kolasinac SM, Lekić S, Golijan J, Petrović T, Todorović G, Kostić A. Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment. in Environmental Monitoring and Assessment. 2018;190(9).
doi:10.1007/s10661-018-6886-x .

Kolasinac, Stefan M., Lekić, Slavoljub, Golijan, Jelena, Petrović, Tanja, Todorović, Goran, Kostić, Aleksandar, "Bioaccumulation process and health risk assessment of toxic elements in tomato fruit grown under Zn nutrition treatment" in Environmental Monitoring and Assessment, 190, no. 9 (2018),
https://doi.org/10.1007/s10661-018-6886-x . .

TY  - CONF
AU  - Lekić, Slavoljub
AU  - Draganić, Ivana
AU  - Pešić, Vladan
AU  - Kolašinac, Stefan
AU  - Vukadinović, Dijana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6553
AB  - In this paper we discuss the cooperation of the Serbian agricultural institutes, faculties and seed
industry which are deserved for the development of seed science and technology and its
applications in the sector of agriculture. It is particularly emphasized the connection between
research and educational institutions in the field of biotechnology and the importance of their
alienation. In addition, it raised a question concerning the reorganization of the existing
universities and research institutes in Serbia after the Bologna reform.
AB  - Univerzitet i polјoprivredni instituti u drugoj polovini ХХ stoleća izveli su uspešan prenos
znanja iz razvijenih zemalјa u SFRJ. Pre dvadesetak godina zapoĉela je tranzicija drţava istoĉne
i jugoistoĉne Evrope što je dovelo do znaĉajnih promena polјoprivrede i obrazovanja. Mnoga
velika srpska polјoprivredna preduzeća nestala su sa privredne scene a njihovo mesto zauzele su
strane kompanije. Promene su zahvatile i oblast visokog obrazovanja te se danas oseća potreba
da se aktualizuje veza izmeĊu naših univerziteta, istraţivaĉkih istitucija u oblasti polјoprivrede i
industrije semena kao vaţnog dela javnog ţivota. Naime, sredinom prošlog veka na prostoru
SFRJ obrazovana je mreţa polјoprivrednih istraţivaĉkih instituta i preduzeća koja su postigla
znaĉajne uspehe u oblasti selekcije i proizvodnje semena. U senci ovog uspeha ostala je ĉinjenica
da su polјoprivredni instituti od univerziteta preuzeli znaĉajan deo istraţivanja što je ograniĉilo
univerzitete, budući da je veći deo laboratorija i opreme bio u institutima. Univeziteti su zajedno
sa institutima imali sve: studente, nastavnike, laboratorije, istraţivaĉku opremu, ali oni su tada,
kao i danas, bili organizaciono odvojeni te ni jedni ni drugi nisu mogli da ostvare veće rezultate.
U uslovima društvene svojine ovaj naĉin rada bio je odrţiv. Danas je takvo razdvajanje smetnja
njihovom razvoju te se polјoprivredni instituti prilagoĊavaju privredi u kojoj dominira privatna
svojina. Shodno promenama u društvu, postojeća drţavna univerzitetska mreţa pretrpela je
promene primenom bolonjske reforme kojom je srpsko visoko obrazovanje upodoblјeno
eropskom školskom prostoru. Na osnovu dosadašnjeg iskustva naziru se tri scenarija ureĊenja
univerziteta. Prvi je oĉuvanje postojeće organizacije (pluralizam uz tihe, evolutivne promene),
drugi je ukidanje privatnih univerziteta (radikalno rešenje), treći je podela postojećih univerziteta
na manje celine prema nauĉnim oblastima (tehniĉki, biotehnološki i td). Moguća regionalizacija
Srbije nametnuće pitanje poloţaja univerziteta u odnosu na lokalnu samoupravu (gradove) tj. da
li su univerziteti isklјuĉivo nacionalne (drţavne) institucije?
Buduće promene visokog obrazovanja morale bi da poĊu od toga da će se jedan deo instituta
pridruţiti univerzitetima dok će ostali oĉuvati svoju pravnu samostalnost i postati ono što su
uvek i bili – preduzeća koja se bave proizvodnjom i prodajom semenske robe. Ova
transformacija olakšala bi ureĊenje odnosa izmeĊu polјoprivrednih instituta (semenskih
preduzeća), univerziteta i banke bilјnih gena. To je od posebnog znaĉaja budući da u postojećim
istraţivaĉkim programima instituta, koje finansira vlada, nema jasne orijentacije na semenarstvo.
Ne ĉudi što struĉna terminologija u oblasti semenarstva slabo razvijena a što bi se moglo
pobolјšati udruţenim delovanjem predstavnika akademske zajednice i semenske industrije i
podizanjem nivoa domaćih struĉnih ĉasopisa u ovoj oblasti. Pred industrijom semena i
univerzitetom stoje brojna pitanja koja su od znaĉaja za dalјi razvoj semenarstva i polјoprivrede.
PB  - Društvo selekcionera i semenara Republike Srbije
C3  - VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''
T1  - SRPSKA INDUSTRIJA SEMENA I UNIVERZITET POSLE TRANZICIJE
T1  - SERBIAN SEED INDUSTRY AND UNIVERSITY AFTER TRANSITION
EP  - 20
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6553
ER  - 

@conference{
author = "Lekić, Slavoljub and Draganić, Ivana and Pešić, Vladan and Kolašinac, Stefan and Vukadinović, Dijana",
year = "2015",
abstract = "In this paper we discuss the cooperation of the Serbian agricultural institutes, faculties and seed
industry which are deserved for the development of seed science and technology and its
applications in the sector of agriculture. It is particularly emphasized the connection between
research and educational institutions in the field of biotechnology and the importance of their
alienation. In addition, it raised a question concerning the reorganization of the existing
universities and research institutes in Serbia after the Bologna reform., Univerzitet i polјoprivredni instituti u drugoj polovini ХХ stoleća izveli su uspešan prenos
znanja iz razvijenih zemalјa u SFRJ. Pre dvadesetak godina zapoĉela je tranzicija drţava istoĉne
i jugoistoĉne Evrope što je dovelo do znaĉajnih promena polјoprivrede i obrazovanja. Mnoga
velika srpska polјoprivredna preduzeća nestala su sa privredne scene a njihovo mesto zauzele su
strane kompanije. Promene su zahvatile i oblast visokog obrazovanja te se danas oseća potreba
da se aktualizuje veza izmeĊu naših univerziteta, istraţivaĉkih istitucija u oblasti polјoprivrede i
industrije semena kao vaţnog dela javnog ţivota. Naime, sredinom prošlog veka na prostoru
SFRJ obrazovana je mreţa polјoprivrednih istraţivaĉkih instituta i preduzeća koja su postigla
znaĉajne uspehe u oblasti selekcije i proizvodnje semena. U senci ovog uspeha ostala je ĉinjenica
da su polјoprivredni instituti od univerziteta preuzeli znaĉajan deo istraţivanja što je ograniĉilo
univerzitete, budući da je veći deo laboratorija i opreme bio u institutima. Univeziteti su zajedno
sa institutima imali sve: studente, nastavnike, laboratorije, istraţivaĉku opremu, ali oni su tada,
kao i danas, bili organizaciono odvojeni te ni jedni ni drugi nisu mogli da ostvare veće rezultate.
U uslovima društvene svojine ovaj naĉin rada bio je odrţiv. Danas je takvo razdvajanje smetnja
njihovom razvoju te se polјoprivredni instituti prilagoĊavaju privredi u kojoj dominira privatna
svojina. Shodno promenama u društvu, postojeća drţavna univerzitetska mreţa pretrpela je
promene primenom bolonjske reforme kojom je srpsko visoko obrazovanje upodoblјeno
eropskom školskom prostoru. Na osnovu dosadašnjeg iskustva naziru se tri scenarija ureĊenja
univerziteta. Prvi je oĉuvanje postojeće organizacije (pluralizam uz tihe, evolutivne promene),
drugi je ukidanje privatnih univerziteta (radikalno rešenje), treći je podela postojećih univerziteta
na manje celine prema nauĉnim oblastima (tehniĉki, biotehnološki i td). Moguća regionalizacija
Srbije nametnuće pitanje poloţaja univerziteta u odnosu na lokalnu samoupravu (gradove) tj. da
li su univerziteti isklјuĉivo nacionalne (drţavne) institucije?
Buduće promene visokog obrazovanja morale bi da poĊu od toga da će se jedan deo instituta
pridruţiti univerzitetima dok će ostali oĉuvati svoju pravnu samostalnost i postati ono što su
uvek i bili – preduzeća koja se bave proizvodnjom i prodajom semenske robe. Ova
transformacija olakšala bi ureĊenje odnosa izmeĊu polјoprivrednih instituta (semenskih
preduzeća), univerziteta i banke bilјnih gena. To je od posebnog znaĉaja budući da u postojećim
istraţivaĉkim programima instituta, koje finansira vlada, nema jasne orijentacije na semenarstvo.
Ne ĉudi što struĉna terminologija u oblasti semenarstva slabo razvijena a što bi se moglo
pobolјšati udruţenim delovanjem predstavnika akademske zajednice i semenske industrije i
podizanjem nivoa domaćih struĉnih ĉasopisa u ovoj oblasti. Pred industrijom semena i
univerzitetom stoje brojna pitanja koja su od znaĉaja za dalјi razvoj semenarstva i polјoprivrede.",
publisher = "Društvo selekcionera i semenara Republike Srbije",
journal = "VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''",
title = "SRPSKA INDUSTRIJA SEMENA I UNIVERZITET POSLE TRANZICIJE, SERBIAN SEED INDUSTRY AND UNIVERSITY AFTER TRANSITION",
pages = "20-19",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6553"
}

Lekić, S., Draganić, I., Pešić, V., Kolašinac, S.,& Vukadinović, D.. (2015). SRPSKA INDUSTRIJA SEMENA I UNIVERZITET POSLE TRANZICIJE. in VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''
Društvo selekcionera i semenara Republike Srbije., 19-20.
https://hdl.handle.net/21.15107/rcub_agrospace_6553

Lekić S, Draganić I, Pešić V, Kolašinac S, Vukadinović D. SRPSKA INDUSTRIJA SEMENA I UNIVERZITET POSLE TRANZICIJE. in VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''. 2015;:19-20.
https://hdl.handle.net/21.15107/rcub_agrospace_6553 .

Lekić, Slavoljub, Draganić, Ivana, Pešić, Vladan, Kolašinac, Stefan, Vukadinović, Dijana, "SRPSKA INDUSTRIJA SEMENA I UNIVERZITET POSLE TRANZICIJE" in VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE'' (2015):19-20,
https://hdl.handle.net/21.15107/rcub_agrospace_6553 .

TY  - CONF
AU  - Draganić, Ivana
AU  - Kolašinac, Stefan
AU  - Vukadinović, Dijana
AU  - Lekić, Slavoljub
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6552
AB  - Vigour is defined as the potential of seeds to germinate rapidly and uniformly under adverse and
optimum conditions. These criteria imply that vigorous seeds will establish early field stands
which provide competitive advantages against weeds and microflora. These advantages could
lead to higher crop yields. Vigour tests are divided in several categories. Physical tests measure
seed characteristics such as size, weight, and density. Physiological tests use some parameter of
germination or seedling growth - speed of germination, standard germination, accelerated ageing,
cold test, Hiltner test. Biochemical tests observe chemical reactions involved in cellular
maintenance - tetrazolium (dehidrogenase), respiration, GADA (glutamic acid decarboxylase
activity), protein, conductivity (leaching), enzyme activity. Many of the vigour test methods need
to be standardize for specific crop species in the future.
AB  - Ţivotna sposobnost semena definiše se kao potencijal semena da brzo i ujednaĉeno klija u
povolјnim i u nepovolјnim uslovima. Ona izraţava obim oštećenja koja se vremenom nakuplјaju
u semenu. Razliĉiti metodi ispitivanja ţivotne sposobnosti semena izvode se tako da u relativno
kratkom roku, jednostavno i pouzdano daju sliku kvaliteta semena i dopune rezultate dobijene
ispitivanjem klijavosti. Partije semena koje imaju visoku klijavost ali slabo nicanje u polјu imaju
nisku ţivotnu sposobnost. Razlike u ţivotnoj sposobnosti semena takoĊe se odraţavaju na brzinu
klijanja i razvoja klijanaca bilo u povolјnim bilo nepovolјnim uslovima.
Direktna ispitivanja ţivotne sposobnosti mere razlike u pogledu nekog vida klijanja ili rasta,
najĉešće pod stresnim uslovima. Jedna od prednosti ovih ispitivanja je što se njima vrednuju
faktori koji utiĉu na ţivotnu sposobnost kao što su starenje, mehaniĉke povrede i morfološke
osobine semena. Drugo je što ĉesto koriste uslove sliĉne onim u polјu i kratko traju te su
jednostavna za tumaĉenje. Fiziološka ispitivanja odnose se na uticaj starenja semena na klijanje
ili poĉetne etape razvoja klijanca. Ova ispitivanja su modifikovana ispitivanja klijavosti.
Ţivotna sposobnost najjednostavnije se procenjuje kao brzina klijanja a na osnovu energije
klijanja (prvo brojanje) pri standardnom ispitivanju klijavosti. S druge strane, brzina klijanja
moţe se vrednovati izraĉunavanjem srednjeg vremena klijanja, srednje vrednosti klijanja i
maksimalne vrednosti klijanja (seme drvenastih vrsta) te na osnovu ispitivanja rasta klijanca
(suva masa klijanca, duţina izdanka, duţina korenĉića).
Ispitivanja ţivotne sposobnosti semena u nepovolјnim (stresnim) kontrolisanim uslovima
simuliraju dejstvo jednog (više) spolјnih faktora koji deluju u polјu. U ovaj vid ispitivanja
spadaju hladni i Hiltnerov test. Testovi starenja zasnovani su na izglanju semena visokoj
temperaturi i/ili vlaţnosti vazduha u odreĊenom vremenskom periodu. Najpoznatija ispitivanja iz
ove grupe obuhvataju ubrzano starenje semena i kontrolisano propadanje semena.
Indirektna ispitivanja usmerena su na jedan aspekt ţivotne spsobnosti, pre nego na ocenu efekata
više faktora na klijanje ili rast mladog klijanca. Biohemijska ispitivanja spadaju u indirektna
ispitivanja ţivotne sposobnosti i obiĉno prate nivo jedne od posledica starenja. U ovu grupu
ispitivanja spadaju merenje elektroprovodlјivosti sadrţaja osloboĊenog iz semena (pasulј, soja,
bob); zatim procena enzimske aktivnosti u semenu koja se boji tetrazolijumom (ţita, soja,
pamuk, grašak, detelina) ili merenje aktivnosti dekarboksilaze glutaminske kiseline (soja, pasulј).
Struĉna literatura poznaje brojne metaboliĉke markere pogodne za biohemijska ispitivanja
ţivotne sposbnosti što zalazi u genomiku, proteomiku i druge uske discipline koje mogu pruţiti
korisne, merlјive parametre ţivotne sposobnosti semena. Direktne i indirektne metode ispitivanja
ţivotne sposobnosti dalјe će se razvijati kako u pogledu osobina koje prate tako i u pogledu
bilјnih vrsta na koje se primenjuju te je od posebnog znaĉaja njihova sistematizacija i
standardizacija.
PB  - Društvo selekcionera i semenara Republike Srbije
C3  - VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''
T1  - PREGLED METODA ISPITIVANJA ŽIVOTNE SPOSOBNOSTI SEMENA
EP  - 66
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6552
ER  - 

@conference{
author = "Draganić, Ivana and Kolašinac, Stefan and Vukadinović, Dijana and Lekić, Slavoljub",
year = "2015",
abstract = "Vigour is defined as the potential of seeds to germinate rapidly and uniformly under adverse and
optimum conditions. These criteria imply that vigorous seeds will establish early field stands
which provide competitive advantages against weeds and microflora. These advantages could
lead to higher crop yields. Vigour tests are divided in several categories. Physical tests measure
seed characteristics such as size, weight, and density. Physiological tests use some parameter of
germination or seedling growth - speed of germination, standard germination, accelerated ageing,
cold test, Hiltner test. Biochemical tests observe chemical reactions involved in cellular
maintenance - tetrazolium (dehidrogenase), respiration, GADA (glutamic acid decarboxylase
activity), protein, conductivity (leaching), enzyme activity. Many of the vigour test methods need
to be standardize for specific crop species in the future., Ţivotna sposobnost semena definiše se kao potencijal semena da brzo i ujednaĉeno klija u
povolјnim i u nepovolјnim uslovima. Ona izraţava obim oštećenja koja se vremenom nakuplјaju
u semenu. Razliĉiti metodi ispitivanja ţivotne sposobnosti semena izvode se tako da u relativno
kratkom roku, jednostavno i pouzdano daju sliku kvaliteta semena i dopune rezultate dobijene
ispitivanjem klijavosti. Partije semena koje imaju visoku klijavost ali slabo nicanje u polјu imaju
nisku ţivotnu sposobnost. Razlike u ţivotnoj sposobnosti semena takoĊe se odraţavaju na brzinu
klijanja i razvoja klijanaca bilo u povolјnim bilo nepovolјnim uslovima.
Direktna ispitivanja ţivotne sposobnosti mere razlike u pogledu nekog vida klijanja ili rasta,
najĉešće pod stresnim uslovima. Jedna od prednosti ovih ispitivanja je što se njima vrednuju
faktori koji utiĉu na ţivotnu sposobnost kao što su starenje, mehaniĉke povrede i morfološke
osobine semena. Drugo je što ĉesto koriste uslove sliĉne onim u polјu i kratko traju te su
jednostavna za tumaĉenje. Fiziološka ispitivanja odnose se na uticaj starenja semena na klijanje
ili poĉetne etape razvoja klijanca. Ova ispitivanja su modifikovana ispitivanja klijavosti.
Ţivotna sposobnost najjednostavnije se procenjuje kao brzina klijanja a na osnovu energije
klijanja (prvo brojanje) pri standardnom ispitivanju klijavosti. S druge strane, brzina klijanja
moţe se vrednovati izraĉunavanjem srednjeg vremena klijanja, srednje vrednosti klijanja i
maksimalne vrednosti klijanja (seme drvenastih vrsta) te na osnovu ispitivanja rasta klijanca
(suva masa klijanca, duţina izdanka, duţina korenĉića).
Ispitivanja ţivotne sposobnosti semena u nepovolјnim (stresnim) kontrolisanim uslovima
simuliraju dejstvo jednog (više) spolјnih faktora koji deluju u polјu. U ovaj vid ispitivanja
spadaju hladni i Hiltnerov test. Testovi starenja zasnovani su na izglanju semena visokoj
temperaturi i/ili vlaţnosti vazduha u odreĊenom vremenskom periodu. Najpoznatija ispitivanja iz
ove grupe obuhvataju ubrzano starenje semena i kontrolisano propadanje semena.
Indirektna ispitivanja usmerena su na jedan aspekt ţivotne spsobnosti, pre nego na ocenu efekata
više faktora na klijanje ili rast mladog klijanca. Biohemijska ispitivanja spadaju u indirektna
ispitivanja ţivotne sposobnosti i obiĉno prate nivo jedne od posledica starenja. U ovu grupu
ispitivanja spadaju merenje elektroprovodlјivosti sadrţaja osloboĊenog iz semena (pasulј, soja,
bob); zatim procena enzimske aktivnosti u semenu koja se boji tetrazolijumom (ţita, soja,
pamuk, grašak, detelina) ili merenje aktivnosti dekarboksilaze glutaminske kiseline (soja, pasulј).
Struĉna literatura poznaje brojne metaboliĉke markere pogodne za biohemijska ispitivanja
ţivotne sposbnosti što zalazi u genomiku, proteomiku i druge uske discipline koje mogu pruţiti
korisne, merlјive parametre ţivotne sposobnosti semena. Direktne i indirektne metode ispitivanja
ţivotne sposobnosti dalјe će se razvijati kako u pogledu osobina koje prate tako i u pogledu
bilјnih vrsta na koje se primenjuju te je od posebnog znaĉaja njihova sistematizacija i
standardizacija.",
publisher = "Društvo selekcionera i semenara Republike Srbije",
journal = "VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''",
title = "PREGLED METODA ISPITIVANJA ŽIVOTNE SPOSOBNOSTI SEMENA",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6552"
}

Draganić, I., Kolašinac, S., Vukadinović, D.,& Lekić, S.. (2015). PREGLED METODA ISPITIVANJA ŽIVOTNE SPOSOBNOSTI SEMENA. in VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''
Društvo selekcionera i semenara Republike Srbije., 66-66.
https://hdl.handle.net/21.15107/rcub_agrospace_6552

Draganić I, Kolašinac S, Vukadinović D, Lekić S. PREGLED METODA ISPITIVANJA ŽIVOTNE SPOSOBNOSTI SEMENA. in VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE''. 2015;:66-66.
https://hdl.handle.net/21.15107/rcub_agrospace_6552 .

Draganić, Ivana, Kolašinac, Stefan, Vukadinović, Dijana, Lekić, Slavoljub, "PREGLED METODA ISPITIVANJA ŽIVOTNE SPOSOBNOSTI SEMENA" in VIII NAUĈNO – STRUĈNI SKUP IZ SELEKCIJE I SEMENARSTVA “GENETIĈKI RESURSI, OPLEMENJIVANJE I SEMENARSTVO U POLJOPRIVREDI SRBIJE-STANJE I PERSPEKTIVE'' (2015):66-66,
https://hdl.handle.net/21.15107/rcub_agrospace_6552 .