Effect of plant growth-promoting Rhizobacteria on the germination of field dodder (Cuscuta campestris Yunck.) and alfalfa

Abstract

To examine the impact of microorganisms (PGPR-Planth Growth Promoting Rhizobacteria) on seed germination of field dodder (Cuscuta campestris Yunck.) and alfalfa (Medicago sativa L.) is the most common hosts of this parasite plants were used for bacterial culture: Bacillus licheniformis, B. pumilus, B. amyloliquefaciens, B. megaterium and A. chroococcum Ps 1 were isolated from the rhizosphere of different plants grown and stored in fridge at a temperature of 4°C until use. Seeds collected field dodder during autumn 2008. in the area of Šabac. Every day for a period of nine days is done counting germination seeds. All treatments were done in four repetitions, and the whole experiment was repeated twice. Based on the results and statistical data processing, in general it can be concluded that all tested treatments (MO1 - B. licheniformis, MO2 - B. pumilus, MO3 - B. amyloliquefaciens, MO4 - B. megaterium and MO5 - A. chroococcum Ps 1) were similar effect on the germination seed of field dodder and alfalfa. Treatments of bacterial culture of Bacillus demonstrated different inhibitory effect on seed germination, field dodder while the same treatment of bacterial culture of Bacillus are more or less 100% inhibitory alfalfa seed germination. In contrast, the bacterial cultures with A. chroococcum has acted in stimulating germination field dodder and alfalfa.

Za ispitivanje uticaja zemljišnih mikroorganizama (PGPR- Planth Growth Promoting Rhizobacteria) na klijanje semena lucerke (Medicago sativa L.) i viline kosice (Cuscuta campestris Yunck.), koja najčešće parazitira na ovoj gajenoj leguminozi korišćene su bakterijske kulture: Bacillus licheniformis, B. pumilus, B. amyloliquefaciens, B. megatherium ZP6 i A. chroococcum Ps1. Bakterijske kulture B. licheniformis, B. pumilus i B. amyloliquefaciens su izolovane iz stajnjaka; B. megatherium ZP6 iz rizosfere kukuruza; a A. chroococcum Ps1 iz rizosfere pšenice i čuvane su u frižideru na temperaturi od 4°C do upotrebe. Seme viline kosice prikupljeno je tokom avgusta meseca 2008. godine sa useva lucerke na području Šapca. Svakodnevno u periodu od devet dana rađeno je brojanje proklijalih semena. Svi tretmani su rađeni u četiri ponavljanja i ceo ogled je ponovljen dva puta. Na osnovu dobijenih rezultata i statističke obrade podataka, generalno se može konstatovati da su svi ispitivani tretmani: MO1 - B. licheniformis, MO2 - B. pumilus, MO3 - B. amyloliquefaciens, MO4 - B. megatherium ZP6 i MO5 - A. chroococcum Ps1 imali sličan efekat na klijanje semena viline kosice i lucerke. Bakterijske kulture različitih vrsta roda Bacillus su ispoljile različit inhibitorni efekat na klijanje semena viline kosice, dok su uglavnom potpuno (100%) inhibirali klijanje semena lucerke. Suprotno tome, bakterijska kultura sa A. chroococcum Ps1 je stimulativno delovala na klijanje semena obe vrste.