TY  - JOUR
AU  - Petrović, Slobodan
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Aleksić, Nevenka
AU  - Maletić, Jelena
AU  - Ristanić, Marko
AU  - Stanimirović, Zoran
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5770
AB  - The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control-group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and Se-GPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperature-humidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals.
PB  - Faculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria
T1  - The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions
EP  - 470
IS  - 4
SP  - 453
VL  - 70
DO  - 10.2478/acve-2020-0034
ER  - 

@article{
author = "Petrović, Slobodan and Maletić, Milan and Lakić, Nada and Aleksić, Nevenka and Maletić, Jelena and Ristanić, Marko and Stanimirović, Zoran",
year = "2021",
abstract = "The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control-group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and Se-GPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperature-humidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria",
title = "The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions",
pages = "470-453",
number = "4",
volume = "70",
doi = "10.2478/acve-2020-0034"
}

Petrović, S., Maletić, M., Lakić, N., Aleksić, N., Maletić, J., Ristanić, M.,& Stanimirović, Z.. (2021). The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions. in Acta Veterinaria
Faculty of Veterinary Medicine, University of Belgrade., 70(4), 453-470.
https://doi.org/10.2478/acve-2020-0034

Petrović S, Maletić M, Lakić N, Aleksić N, Maletić J, Ristanić M, Stanimirović Z. The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions. in Acta Veterinaria. 2021;70(4):453-470.
doi:10.2478/acve-2020-0034 .

Petrović, Slobodan, Maletić, Milan, Lakić, Nada, Aleksić, Nevenka, Maletić, Jelena, Ristanić, Marko, Stanimirović, Zoran, "The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions" in Acta Veterinaria, 70, no. 4 (2021):453-470,
https://doi.org/10.2478/acve-2020-0034 . .

TY  - JOUR
AU  - Maletić, Milan
AU  - Paprikić, Nevres
AU  - Lazarević, Miodrag
AU  - Hodzić, Aida
AU  - Davidović, Vesna
AU  - Stanisić, Ljubodrag
AU  - Stanimirović, Zoran
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5107
AB  - Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle
EP  - 163
IS  - 2
SP  - 153
VL  - 69
DO  - 10.2478/acve-2019-0012
ER  - 

@article{
author = "Maletić, Milan and Paprikić, Nevres and Lazarević, Miodrag and Hodzić, Aida and Davidović, Vesna and Stanisić, Ljubodrag and Stanimirović, Zoran",
year = "2019",
abstract = "Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle",
pages = "163-153",
number = "2",
volume = "69",
doi = "10.2478/acve-2019-0012"
}

Maletić, M., Paprikić, N., Lazarević, M., Hodzić, A., Davidović, V., Stanisić, L.,& Stanimirović, Z.. (2019). Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(2), 153-163.
https://doi.org/10.2478/acve-2019-0012

Maletić M, Paprikić N, Lazarević M, Hodzić A, Davidović V, Stanisić L, Stanimirović Z. Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd. 2019;69(2):153-163.
doi:10.2478/acve-2019-0012 .

Maletić, Milan, Paprikić, Nevres, Lazarević, Miodrag, Hodzić, Aida, Davidović, Vesna, Stanisić, Ljubodrag, Stanimirović, Zoran, "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle" in Acta Veterinaria-Beograd, 69, no. 2 (2019):153-163,
https://doi.org/10.2478/acve-2019-0012 . .

TY  - JOUR
AU  - Bosnjak-Neumuller, Jasna
AU  - Radaković, Milena
AU  - Djelić, Ninoslav
AU  - Vuković-Gačić, Branka
AU  - Dajić-Stevanović, Zora
AU  - Kolarević, Stoimir
AU  - Misić, Danijela
AU  - Stanković, Milan
AU  - Knežević-Vukčević, Jelena
AU  - Spremo-Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4504
AB  - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).
PB  - NLM (Medline)
T2  - Pakistan journal of pharmaceutical sciences
T1  - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes
EP  - 634
IS  - 2
SP  - 625
VL  - 30
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4504
ER  - 

@article{
author = "Bosnjak-Neumuller, Jasna and Radaković, Milena and Djelić, Ninoslav and Vuković-Gačić, Branka and Dajić-Stevanović, Zora and Kolarević, Stoimir and Misić, Danijela and Stanković, Milan and Knežević-Vukčević, Jelena and Spremo-Potparević, Biljana and Stanimirović, Zoran",
year = "2017",
abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of agemg, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T-3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2-picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73 +/- 1.80 mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59 +/- 0.95 g/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0 mg/ml N. rtanjensis methanol extracts mixture with 100 mu M of T-3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS/MS).",
publisher = "NLM (Medline)",
journal = "Pakistan journal of pharmaceutical sciences",
title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes",
pages = "634-625",
number = "2",
volume = "30",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504"
}

Bosnjak-Neumuller, J., Radaković, M., Djelić, N., Vuković-Gačić, B., Dajić-Stevanović, Z., Kolarević, S., Misić, D., Stanković, M., Knežević-Vukčević, J., Spremo-Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences
NLM (Medline)., 30(2), 625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504

Bosnjak-Neumuller J, Radaković M, Djelić N, Vuković-Gačić B, Dajić-Stevanović Z, Kolarević S, Misić D, Stanković M, Knežević-Vukčević J, Spremo-Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. in Pakistan journal of pharmaceutical sciences. 2017;30(2):625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

Bosnjak-Neumuller, Jasna, Radaković, Milena, Djelić, Ninoslav, Vuković-Gačić, Branka, Dajić-Stevanović, Zora, Kolarević, Stoimir, Misić, Danijela, Stanković, Milan, Knežević-Vukčević, Jelena, Spremo-Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes" in Pakistan journal of pharmaceutical sciences, 30, no. 2 (2017):625-634,
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

TY  - JOUR
AU  - Bosnjak-Neumuller, Jasna
AU  - Djelić, Ninoslav
AU  - Radaković, Milena
AU  - Kolarević, Stoimir
AU  - Mitić-Culafić, Dragana
AU  - Dajić-Stevanović, Zora
AU  - Vuković-Gačić, Branka
AU  - Knežević-Vukčević, Jelena
AU  - Stanimirović, Zoran
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4409
AB  - There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro
EP  - 397
IS  - 2
SP  - 387
VL  - 49
DO  - 10.2298/GENSR1702387B
ER  - 

@article{
author = "Bosnjak-Neumuller, Jasna and Djelić, Ninoslav and Radaković, Milena and Kolarević, Stoimir and Mitić-Culafić, Dragana and Dajić-Stevanović, Zora and Vuković-Gačić, Branka and Knežević-Vukčević, Jelena and Stanimirović, Zoran",
year = "2017",
abstract = "There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro",
pages = "397-387",
number = "2",
volume = "49",
doi = "10.2298/GENSR1702387B"
}

Bosnjak-Neumuller, J., Djelić, N., Radaković, M., Kolarević, S., Mitić-Culafić, D., Dajić-Stevanović, Z., Vuković-Gačić, B., Knežević-Vukčević, J.,& Stanimirović, Z.. (2017). Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 387-397.
https://doi.org/10.2298/GENSR1702387B

Bosnjak-Neumuller J, Djelić N, Radaković M, Kolarević S, Mitić-Culafić D, Dajić-Stevanović Z, Vuković-Gačić B, Knežević-Vukčević J, Stanimirović Z. Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro. in Genetika. 2017;49(2):387-397.
doi:10.2298/GENSR1702387B .

Bosnjak-Neumuller, Jasna, Djelić, Ninoslav, Radaković, Milena, Kolarević, Stoimir, Mitić-Culafić, Dragana, Dajić-Stevanović, Zora, Vuković-Gačić, Branka, Knežević-Vukčević, Jelena, Stanimirović, Zoran, "Genotoxicity of triiodothyronine: effects on salmonella typhimurium ta100 and human lymphocytes in vitro" in Genetika, 49, no. 2 (2017):387-397,
https://doi.org/10.2298/GENSR1702387B . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Stancić, Ivan
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Kanacki, Zdenko
AU  - Stanković, Branislav
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3765
AB  - The aim of the present study was to investigate the possibility of increasing boar's reproductive exploitation by using AI doses of doubly reduced volume and sperm count in the intrauterine AI procedure. The experiment was conducted at a commercial pig farm in Serbia in 2014. Classic intracervical insemination (ICI) was performed by using 50 mL or 100 mL volume doses containing 4 x 10(9) or 2 x 10(9) progressively motile spermatozoa. The same volumes and sperm numbers per dose were used with intrauterine insemination (IUI). Each dose combination was used to inseminate 30 sows. Intrauterine insemination with AI doses of reduced volume (50 mL) and sperm count (2 x 10(9)) did not produce a statistically significant difference (P  lt  0.05) in the farrowing rate (76.7%) as compared with 4 x 10(9) spermatozoa in the same volume (83.3%) or with insemination by doses of 100 mL with a 2 x 10(9) (83.3%) or a 4 x 10(9) sperm count (86.7%). The number of live-born piglets (10.82) was larger following IUI using a 50 mL volume dose with a 2 x 10(9) sperm count as compared with ICI with the same AI dose volume and sperm count (9.85). The results show that the use of reduced AI dosages provides an opportunity for the swine industry to considerably exploit the reproductive potential of genetically superior boars.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary & Animal Sciences
T1  - Sow fertility after insemination with varying doses of volume and spermatozoa count
EP  - 713
IS  - 6
SP  - 709
VL  - 39
DO  - 10.3906/vet-1503-50
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Stancić, Ivan and Radović, Ivan and Jotanović, Stoja and Kanacki, Zdenko and Stanković, Branislav",
year = "2015",
abstract = "The aim of the present study was to investigate the possibility of increasing boar's reproductive exploitation by using AI doses of doubly reduced volume and sperm count in the intrauterine AI procedure. The experiment was conducted at a commercial pig farm in Serbia in 2014. Classic intracervical insemination (ICI) was performed by using 50 mL or 100 mL volume doses containing 4 x 10(9) or 2 x 10(9) progressively motile spermatozoa. The same volumes and sperm numbers per dose were used with intrauterine insemination (IUI). Each dose combination was used to inseminate 30 sows. Intrauterine insemination with AI doses of reduced volume (50 mL) and sperm count (2 x 10(9)) did not produce a statistically significant difference (P  lt  0.05) in the farrowing rate (76.7%) as compared with 4 x 10(9) spermatozoa in the same volume (83.3%) or with insemination by doses of 100 mL with a 2 x 10(9) (83.3%) or a 4 x 10(9) sperm count (86.7%). The number of live-born piglets (10.82) was larger following IUI using a 50 mL volume dose with a 2 x 10(9) sperm count as compared with ICI with the same AI dose volume and sperm count (9.85). The results show that the use of reduced AI dosages provides an opportunity for the swine industry to considerably exploit the reproductive potential of genetically superior boars.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary & Animal Sciences",
title = "Sow fertility after insemination with varying doses of volume and spermatozoa count",
pages = "713-709",
number = "6",
volume = "39",
doi = "10.3906/vet-1503-50"
}

Apić, J., Vakanjac, S., Stancić, I., Radović, I., Jotanović, S., Kanacki, Z.,& Stanković, B.. (2015). Sow fertility after insemination with varying doses of volume and spermatozoa count. in Turkish Journal of Veterinary & Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 39(6), 709-713.
https://doi.org/10.3906/vet-1503-50

Apić J, Vakanjac S, Stancić I, Radović I, Jotanović S, Kanacki Z, Stanković B. Sow fertility after insemination with varying doses of volume and spermatozoa count. in Turkish Journal of Veterinary & Animal Sciences. 2015;39(6):709-713.
doi:10.3906/vet-1503-50 .

Apić, Jelena, Vakanjac, Slobodanka, Stancić, Ivan, Radović, Ivan, Jotanović, Stoja, Kanacki, Zdenko, Stanković, Branislav, "Sow fertility after insemination with varying doses of volume and spermatozoa count" in Turkish Journal of Veterinary & Animal Sciences, 39, no. 6 (2015):709-713,
https://doi.org/10.3906/vet-1503-50 . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Zivkov-Balos, Milica
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Stanković, Branislav
AU  - Jajić, Igor
AU  - Maletić, Milan
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3762
AB  - Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p lt 0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p lt 0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p lt 0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p lt 0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.
PB  - Slovak Univ Agriculture Nitra, Nitra
T2  - Journal of Microbiology Biotechnology and Food Sciences
T1  - Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days
EP  - 3
SP  - 1
VL  - 4
DO  - 10.15414/jmbfs.2015.4.special2.1-3
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Radović, Ivan and Jotanović, Stoja and Zivkov-Balos, Milica and Milovanović, Aleksandar and Barna, Tomislav and Stanković, Branislav and Jajić, Igor and Maletić, Milan",
year = "2015",
abstract = "Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p lt 0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p lt 0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p lt 0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p lt 0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.",
publisher = "Slovak Univ Agriculture Nitra, Nitra",
journal = "Journal of Microbiology Biotechnology and Food Sciences",
title = "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days",
pages = "3-1",
volume = "4",
doi = "10.15414/jmbfs.2015.4.special2.1-3"
}

Apić, J., Vakanjac, S., Radović, I., Jotanović, S., Zivkov-Balos, M., Milovanović, A., Barna, T., Stanković, B., Jajić, I.,& Maletić, M.. (2015). Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences
Slovak Univ Agriculture Nitra, Nitra., 4, 1-3.
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3

Apić J, Vakanjac S, Radović I, Jotanović S, Zivkov-Balos M, Milovanović A, Barna T, Stanković B, Jajić I, Maletić M. Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences. 2015;4:1-3.
doi:10.15414/jmbfs.2015.4.special2.1-3 .

Apić, Jelena, Vakanjac, Slobodanka, Radović, Ivan, Jotanović, Stoja, Zivkov-Balos, Milica, Milovanović, Aleksandar, Barna, Tomislav, Stanković, Branislav, Jajić, Igor, Maletić, Milan, "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days" in Journal of Microbiology Biotechnology and Food Sciences, 4 (2015):1-3,
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3 . .

TY  - JOUR
AU  - Radović, D.I.
AU  - Lazarević, Kristina B.
AU  - Trifković, Jelena D.
AU  - Andrić, Filip Lj.
AU  - Tešić, Živoslav
AU  - Andjelković, Ivan B.
AU  - Nedić, Nebojša
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Curcić, B.P.M.
AU  - Milojković-Opsenica, Dušanka M.
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3462
AB  - GIS is a computer-based system to input, store, manipulate, analyze and output spatially referenced data. There is a huge range application of GIS that generally sets out to fulfill: mapping, measurement, monitoring, modeling and management. In this study, GIS technology was used for the regional recognition of origin and distribution patterns of multifloral honey chemical traits in Serbia. This included organizing and analyzing the spatial and attributive data of 164 honey samples collected from different regions of Serbia during the harvesting season of 2009. Multifloral honey was characterized in regards to mineral composition, sugar content and basic physicochemical properties. The kriging method of Geostatistical Analyst was used for interpolation to predict values of a sampled variable over the whole territory of Serbia
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Gis techology in regional recognition of the distribution pattern of multifloral honey: the chemical traits in Serbia
EP  - 946
IS  - 2
SP  - 935
VL  - 66
DO  - 10.2298/ABS1402935R
ER  - 

@article{
author = "Radović, D.I. and Lazarević, Kristina B. and Trifković, Jelena D. and Andrić, Filip Lj. and Tešić, Živoslav and Andjelković, Ivan B. and Nedić, Nebojša and Stanimirović, Zoran and Stevanović, Jevrosima and Curcić, B.P.M. and Milojković-Opsenica, Dušanka M.",
year = "2014",
abstract = "GIS is a computer-based system to input, store, manipulate, analyze and output spatially referenced data. There is a huge range application of GIS that generally sets out to fulfill: mapping, measurement, monitoring, modeling and management. In this study, GIS technology was used for the regional recognition of origin and distribution patterns of multifloral honey chemical traits in Serbia. This included organizing and analyzing the spatial and attributive data of 164 honey samples collected from different regions of Serbia during the harvesting season of 2009. Multifloral honey was characterized in regards to mineral composition, sugar content and basic physicochemical properties. The kriging method of Geostatistical Analyst was used for interpolation to predict values of a sampled variable over the whole territory of Serbia",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Gis techology in regional recognition of the distribution pattern of multifloral honey: the chemical traits in Serbia",
pages = "946-935",
number = "2",
volume = "66",
doi = "10.2298/ABS1402935R"
}

Radović, D.I., Lazarević, K. B., Trifković, J. D., Andrić, F. Lj., Tešić, Ž., Andjelković, I. B., Nedić, N., Stanimirović, Z., Stevanović, J., Curcić, B.P.M.,& Milojković-Opsenica, D. M.. (2014). Gis techology in regional recognition of the distribution pattern of multifloral honey: the chemical traits in Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 935-946.
https://doi.org/10.2298/ABS1402935R

Radović D, Lazarević KB, Trifković JD, Andrić FL, Tešić Ž, Andjelković IB, Nedić N, Stanimirović Z, Stevanović J, Curcić B, Milojković-Opsenica DM. Gis techology in regional recognition of the distribution pattern of multifloral honey: the chemical traits in Serbia. in Archives of Biological Sciences. 2014;66(2):935-946.
doi:10.2298/ABS1402935R .

Radović, D.I., Lazarević, Kristina B., Trifković, Jelena D., Andrić, Filip Lj., Tešić, Živoslav, Andjelković, Ivan B., Nedić, Nebojša, Stanimirović, Zoran, Stevanović, Jevrosima, Curcić, B.P.M., Milojković-Opsenica, Dušanka M., "Gis techology in regional recognition of the distribution pattern of multifloral honey: the chemical traits in Serbia" in Archives of Biological Sciences, 66, no. 2 (2014):935-946,
https://doi.org/10.2298/ABS1402935R . .