The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions
Аутори
Petrović, SlobodanMaletić, Milan
Lakić, Nada
Aleksić, Nevenka
Maletić, Jelena
Ristanić, Marko
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control-group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and Se-GPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<...0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperature-humidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals.
Кључне речи:
antioxidative enzymes / bull / motility / spermИзвор:
Acta Veterinaria, 2021, 70, 4, 453-470Издавач:
- Faculty of Veterinary Medicine, University of Belgrade
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2478/acve-2020-0034
ISSN: 0567-8315
WoS: 000606333900004
Scopus: 2-s2.0-85099337503
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Petrović, Slobodan AU - Maletić, Milan AU - Lakić, Nada AU - Aleksić, Nevenka AU - Maletić, Jelena AU - Ristanić, Marko AU - Stanimirović, Zoran PY - 2021 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5770 AB - The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control-group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and Se-GPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperature-humidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals. PB - Faculty of Veterinary Medicine, University of Belgrade T2 - Acta Veterinaria T1 - The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions EP - 470 IS - 4 SP - 453 VL - 70 DO - 10.2478/acve-2020-0034 ER -
@article{ author = "Petrović, Slobodan and Maletić, Milan and Lakić, Nada and Aleksić, Nevenka and Maletić, Jelena and Ristanić, Marko and Stanimirović, Zoran", year = "2021", abstract = "The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control-group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and Se-GPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperature-humidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals.", publisher = "Faculty of Veterinary Medicine, University of Belgrade", journal = "Acta Veterinaria", title = "The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions", pages = "470-453", number = "4", volume = "70", doi = "10.2478/acve-2020-0034" }
Petrović, S., Maletić, M., Lakić, N., Aleksić, N., Maletić, J., Ristanić, M.,& Stanimirović, Z.. (2021). The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions. in Acta Veterinaria Faculty of Veterinary Medicine, University of Belgrade., 70(4), 453-470. https://doi.org/10.2478/acve-2020-0034
Petrović S, Maletić M, Lakić N, Aleksić N, Maletić J, Ristanić M, Stanimirović Z. The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions. in Acta Veterinaria. 2021;70(4):453-470. doi:10.2478/acve-2020-0034 .
Petrović, Slobodan, Maletić, Milan, Lakić, Nada, Aleksić, Nevenka, Maletić, Jelena, Ristanić, Marko, Stanimirović, Zoran, "The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions" in Acta Veterinaria, 70, no. 4 (2021):453-470, https://doi.org/10.2478/acve-2020-0034 . .