TY  - CONF
AU  - Peter, K.
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - Park, E.
AU  - Gaskins, Verneta L.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2664
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Host modification of Penicillium solitum during postharvest decay of apple fruit
EP  - S141
IS  - 6
SP  - S141
VL  - 101
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2664
ER  - 

@conference{
author = "Peter, K. and Jurick, Wayne M., II and Vico, Ivana and Park, E. and Gaskins, Verneta L. and Janisiewicz, Wojciech and Conway, William S.",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Host modification of Penicillium solitum during postharvest decay of apple fruit",
pages = "S141-S141",
number = "6",
volume = "101",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2664"
}

Peter, K., Jurick, W. M., I., Vico, I., Park, E., Gaskins, V. L., Janisiewicz, W.,& Conway, W. S.. (2011). Host modification of Penicillium solitum during postharvest decay of apple fruit. in Phytopathology
Amer Phytopathological Soc, St Paul., 101(6), S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_2664

Peter K, Jurick WMI, Vico I, Park E, Gaskins VL, Janisiewicz W, Conway WS. Host modification of Penicillium solitum during postharvest decay of apple fruit. in Phytopathology. 2011;101(6):S141-S141.
https://hdl.handle.net/21.15107/rcub_agrospace_2664 .

Peter, K., Jurick, Wayne M., II, Vico, Ivana, Park, E., Gaskins, Verneta L., Janisiewicz, Wojciech, Conway, William S., "Host modification of Penicillium solitum during postharvest decay of apple fruit" in Phytopathology, 101, no. 6 (2011):S141-S141,
https://hdl.handle.net/21.15107/rcub_agrospace_2664 .

TY  - JOUR
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - Gaskins, Verneta L.
AU  - Garrett, Wesley M.
AU  - Whitaker, Bruce D.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2375
AB  - A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified PG is of fungal origin. The purified enzyme had a molecular mass of 41 kDa and a pI of 7.8. Activity of the PG was not associated with a glycosylated protein. The enzyme was active over a broad pH range from 3 to 6, with optimal activity at 4.5 in sodium citrate and sodium acetate buffers. The optimal temperature for activity was 37 degrees C but the enzyme was also active at 0, 5, 10, 20, and 50 degrees C. Thin-layer chromatographic analysis of PG hydrolysis products showed that the enzyme exhibits endo- and exo-activity. The purified enzyme macerated tissue in vitro causing approximate to 30% reduction in mass of pear plugs compared with approximate to 17% reduction for apple. Additionally, it produced 1.5-fold more soluble polyuronides on pear than apple tissue. This work shows for the first time the production of a PG by P. expansum during postharvest decay of pear fruit is different from the previously described PG produced in decayed apple fruit by the same pathogen.
PB  - Amer Phytopathological Soc, St Paul
T2  - Phytopathology
T1  - Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear
EP  - 48
IS  - 1
SP  - 42
VL  - 100
DO  - 10.1094/PHYTO-100-1-0042
ER  - 

@article{
author = "Jurick, Wayne M., II and Vico, Ivana and Gaskins, Verneta L. and Garrett, Wesley M. and Whitaker, Bruce D. and Janisiewicz, Wojciech and Conway, William S.",
year = "2010",
abstract = "A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified PG is of fungal origin. The purified enzyme had a molecular mass of 41 kDa and a pI of 7.8. Activity of the PG was not associated with a glycosylated protein. The enzyme was active over a broad pH range from 3 to 6, with optimal activity at 4.5 in sodium citrate and sodium acetate buffers. The optimal temperature for activity was 37 degrees C but the enzyme was also active at 0, 5, 10, 20, and 50 degrees C. Thin-layer chromatographic analysis of PG hydrolysis products showed that the enzyme exhibits endo- and exo-activity. The purified enzyme macerated tissue in vitro causing approximate to 30% reduction in mass of pear plugs compared with approximate to 17% reduction for apple. Additionally, it produced 1.5-fold more soluble polyuronides on pear than apple tissue. This work shows for the first time the production of a PG by P. expansum during postharvest decay of pear fruit is different from the previously described PG produced in decayed apple fruit by the same pathogen.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear",
pages = "48-42",
number = "1",
volume = "100",
doi = "10.1094/PHYTO-100-1-0042"
}

Jurick, W. M., I., Vico, I., Gaskins, V. L., Garrett, W. M., Whitaker, B. D., Janisiewicz, W.,& Conway, W. S.. (2010). Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear. in Phytopathology
Amer Phytopathological Soc, St Paul., 100(1), 42-48.
https://doi.org/10.1094/PHYTO-100-1-0042

Jurick WMI, Vico I, Gaskins VL, Garrett WM, Whitaker BD, Janisiewicz W, Conway WS. Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear. in Phytopathology. 2010;100(1):42-48.
doi:10.1094/PHYTO-100-1-0042 .

Jurick, Wayne M., II, Vico, Ivana, Gaskins, Verneta L., Garrett, Wesley M., Whitaker, Bruce D., Janisiewicz, Wojciech, Conway, William S., "Purification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pear" in Phytopathology, 100, no. 1 (2010):42-48,
https://doi.org/10.1094/PHYTO-100-1-0042 . .

TY  - JOUR
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - McEvoy, James L.
AU  - Whitaker, Bruce D.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1987
AB  - Polygalacturonase (PG) was extracted and purified from decayed 'Golden Delicious' apple fruit inoculated with Penicillium solitum. Ammonium sulfate, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. The purified PG most likely originates from the fungus because PG activity from healthy and wounded apple tissue was undetectable. Analysis of cation exchange-purified material using sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single 50-kDa band. The enzyme was active over a broad pH range (3 to 7), with optimal activity between pH 4 and 5. PG was highly active at 20 and 37 degrees C but was also detectable at 2, 50, and 75 degrees C. Divalent cations affected PG enzyme activity; Mg and Fe increased, whereas Ca and Mn reduced activity in vitro. Thin-layer chromatographic separation of hydrolysis products and data from a PG plate activity assay based on staining with ruthenium red showed that the enzyme exhibits both exo and endo activity. Purified PG incubated with intact apple fruit tissue in vitro caused a 30% reduction in mass after 48 h, suggesting a role in P. solitum-mediated decay of apple fruit.
PB  - Amer Phytopathological Soc, St Paul
T2  - Phytopathology
T1  - Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit
EP  - 641
IS  - 6
SP  - 636
VL  - 99
DO  - 10.1094/PHYTO-99-6-0636
ER  - 

@article{
author = "Jurick, Wayne M., II and Vico, Ivana and McEvoy, James L. and Whitaker, Bruce D. and Janisiewicz, Wojciech and Conway, William S.",
year = "2009",
abstract = "Polygalacturonase (PG) was extracted and purified from decayed 'Golden Delicious' apple fruit inoculated with Penicillium solitum. Ammonium sulfate, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. The purified PG most likely originates from the fungus because PG activity from healthy and wounded apple tissue was undetectable. Analysis of cation exchange-purified material using sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single 50-kDa band. The enzyme was active over a broad pH range (3 to 7), with optimal activity between pH 4 and 5. PG was highly active at 20 and 37 degrees C but was also detectable at 2, 50, and 75 degrees C. Divalent cations affected PG enzyme activity; Mg and Fe increased, whereas Ca and Mn reduced activity in vitro. Thin-layer chromatographic separation of hydrolysis products and data from a PG plate activity assay based on staining with ruthenium red showed that the enzyme exhibits both exo and endo activity. Purified PG incubated with intact apple fruit tissue in vitro caused a 30% reduction in mass after 48 h, suggesting a role in P. solitum-mediated decay of apple fruit.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit",
pages = "641-636",
number = "6",
volume = "99",
doi = "10.1094/PHYTO-99-6-0636"
}

Jurick, W. M., I., Vico, I., McEvoy, J. L., Whitaker, B. D., Janisiewicz, W.,& Conway, W. S.. (2009). Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit. in Phytopathology
Amer Phytopathological Soc, St Paul., 99(6), 636-641.
https://doi.org/10.1094/PHYTO-99-6-0636

Jurick WMI, Vico I, McEvoy JL, Whitaker BD, Janisiewicz W, Conway WS. Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit. in Phytopathology. 2009;99(6):636-641.
doi:10.1094/PHYTO-99-6-0636 .

Jurick, Wayne M., II, Vico, Ivana, McEvoy, James L., Whitaker, Bruce D., Janisiewicz, Wojciech, Conway, William S., "Isolation, Purification, and Characterization of a Polygalacturonase Produced in Penicillium solitum-Decayed 'Golden Delicious' Apple Fruit" in Phytopathology, 99, no. 6 (2009):636-641,
https://doi.org/10.1094/PHYTO-99-6-0636 . .

TY  - CONF
AU  - Jurick, Wayne M., II
AU  - Vico, Ivana
AU  - McEvoy, J.L.
AU  - Janisiewicz, Wojciech
AU  - Conway, William S.
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1806
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh)
EP  - S77
IS  - 6
SP  - S77
VL  - 98
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1806
ER  - 

@conference{
author = "Jurick, Wayne M., II and Vico, Ivana and McEvoy, J.L. and Janisiewicz, Wojciech and Conway, William S.",
year = "2008",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh)",
pages = "S77-S77",
number = "6",
volume = "98",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1806"
}

Jurick, W. M., I., Vico, I., McEvoy, J.L., Janisiewicz, W.,& Conway, W. S.. (2008). Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh). in Phytopathology
Amer Phytopathological Soc, St Paul., 98(6), S77-S77.
https://hdl.handle.net/21.15107/rcub_agrospace_1806

Jurick WMI, Vico I, McEvoy J, Janisiewicz W, Conway WS. Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh). in Phytopathology. 2008;98(6):S77-S77.
https://hdl.handle.net/21.15107/rcub_agrospace_1806 .

Jurick, Wayne M., II, Vico, Ivana, McEvoy, J.L., Janisiewicz, Wojciech, Conway, William S., "Isolation, purification and biochemical characterization of a polygalacturonase produced by Penicillium solitum in 'Golden Delicious' apple (Malus domestica Borkh)" in Phytopathology, 98, no. 6 (2008):S77-S77,
https://hdl.handle.net/21.15107/rcub_agrospace_1806 .