TY  - JOUR
AU  - Zorc, Minja
AU  - Škorput, Dubravko
AU  - Gvozdanović, Kristina
AU  - Margeta, Polona
AU  - Karolyi, Danijel
AU  - Luković, Zoran
AU  - Salajpal, Krešimir
AU  - Savić, Radomir
AU  - Muñoz, Maria
AU  - Bovo, Samuele
AU  - Djurkin Kušec, Ivona
AU  - Radović, Čedomir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6076
AB  - The importance of local breeds as genetic reservoirs of valuable genetic variation is well established. Pig breeding in Central and South-Eastern Europe has a long tradition that led to the formation of several local pig breeds. In the present study, genetic diversity parameters were analysed in six autochthonous pig breeds from Slovenia, Croatia and Serbia (Banija spotted, Black Slavonian, Turopolje pig, Swallow-bellied Mangalitsa, Moravka and Krskopolje pig). Animals from each of these breeds were genotyped using microsatellites and single nucleotide polymorphisms (SNPs). The results obtained with these two marker systems and those based on pedigree data were compared. In addition, we estimated inbreeding levels based on the distribution of runs of homozygosity (ROH) and identified genomic regions under selection pressure using ROH islands and the integrated haplotype score (iHS). Results: The lowest heterozygosity values calculated from microsatellite and SNP data were observed in the Turopolje pig. The observed heterozygosity was higher than the expected heterozygosity in the Black Slavonian, Moravka and Turopolje pig. Both types of markers allowed us to distinguish clusters of individuals belonging to each breed. The analysis of admixture between breeds revealed potential gene flow between the Mangalitsa and Moravka, and between the Mangalitsa and Black Slavonian, but no introgression events were detected in the Banija spotted and Turopolje pig. The distribution of ROH across the genome was not uniform. Analysis of the ROH islands identified genomic regions with an extremely high frequency of shared ROH within the Swallow-bellied Mangalitsa, which harboured genes associated with cholesterol biosynthesis, fatty acid metabolism and daily weight gain. The iHS approach to detect signatures of selection revealed candidate regions containing genes with potential roles in reproduction traits and disease resistance. Conclusions: Based on the estimation of population parameters obtained from three data sets, we showed the existence of relationships among the six pig breeds analysed here. Analysis of the distribution of ROH allowed us to estimate the level of inbreeding and the extent of homozygous regions in these breeds. The iHS analysis revealed genomic regions potentially associated with phenotypic traits and allowed the detection of genomic regions under selection pressure.
PB  - BioMed Central Ltd
T2  - Genetics Selection Evolution
T1  - Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia
IS  - 1
SP  - 30
VL  - 54
DO  - 10.1186/s12711-022-00718-6
ER  - 

@article{
author = "Zorc, Minja and Škorput, Dubravko and Gvozdanović, Kristina and Margeta, Polona and Karolyi, Danijel and Luković, Zoran and Salajpal, Krešimir and Savić, Radomir and Muñoz, Maria and Bovo, Samuele and Djurkin Kušec, Ivona and Radović, Čedomir",
year = "2022",
abstract = "The importance of local breeds as genetic reservoirs of valuable genetic variation is well established. Pig breeding in Central and South-Eastern Europe has a long tradition that led to the formation of several local pig breeds. In the present study, genetic diversity parameters were analysed in six autochthonous pig breeds from Slovenia, Croatia and Serbia (Banija spotted, Black Slavonian, Turopolje pig, Swallow-bellied Mangalitsa, Moravka and Krskopolje pig). Animals from each of these breeds were genotyped using microsatellites and single nucleotide polymorphisms (SNPs). The results obtained with these two marker systems and those based on pedigree data were compared. In addition, we estimated inbreeding levels based on the distribution of runs of homozygosity (ROH) and identified genomic regions under selection pressure using ROH islands and the integrated haplotype score (iHS). Results: The lowest heterozygosity values calculated from microsatellite and SNP data were observed in the Turopolje pig. The observed heterozygosity was higher than the expected heterozygosity in the Black Slavonian, Moravka and Turopolje pig. Both types of markers allowed us to distinguish clusters of individuals belonging to each breed. The analysis of admixture between breeds revealed potential gene flow between the Mangalitsa and Moravka, and between the Mangalitsa and Black Slavonian, but no introgression events were detected in the Banija spotted and Turopolje pig. The distribution of ROH across the genome was not uniform. Analysis of the ROH islands identified genomic regions with an extremely high frequency of shared ROH within the Swallow-bellied Mangalitsa, which harboured genes associated with cholesterol biosynthesis, fatty acid metabolism and daily weight gain. The iHS approach to detect signatures of selection revealed candidate regions containing genes with potential roles in reproduction traits and disease resistance. Conclusions: Based on the estimation of population parameters obtained from three data sets, we showed the existence of relationships among the six pig breeds analysed here. Analysis of the distribution of ROH allowed us to estimate the level of inbreeding and the extent of homozygous regions in these breeds. The iHS analysis revealed genomic regions potentially associated with phenotypic traits and allowed the detection of genomic regions under selection pressure.",
publisher = "BioMed Central Ltd",
journal = "Genetics Selection Evolution",
title = "Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia",
number = "1",
pages = "30",
volume = "54",
doi = "10.1186/s12711-022-00718-6"
}

Zorc, M., Škorput, D., Gvozdanović, K., Margeta, P., Karolyi, D., Luković, Z., Salajpal, K., Savić, R., Muñoz, M., Bovo, S., Djurkin Kušec, I.,& Radović, Č.. (2022). Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia. in Genetics Selection Evolution
BioMed Central Ltd., 54(1), 30.
https://doi.org/10.1186/s12711-022-00718-6

Zorc M, Škorput D, Gvozdanović K, Margeta P, Karolyi D, Luković Z, Salajpal K, Savić R, Muñoz M, Bovo S, Djurkin Kušec I, Radović Č. Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia. in Genetics Selection Evolution. 2022;54(1):30.
doi:10.1186/s12711-022-00718-6 .

Zorc, Minja, Škorput, Dubravko, Gvozdanović, Kristina, Margeta, Polona, Karolyi, Danijel, Luković, Zoran, Salajpal, Krešimir, Savić, Radomir, Muñoz, Maria, Bovo, Samuele, Djurkin Kušec, Ivona, Radović, Čedomir, "Genetic diversity and population structure of six autochthonous pig breeds from Croatia, Serbia, and Slovenia" in Genetics Selection Evolution, 54, no. 1 (2022):30,
https://doi.org/10.1186/s12711-022-00718-6 . .

TY  - JOUR
AU  - Núñez, Yolanda
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - García-Casco, Juan M.
AU  - Čandek-Potokar, Marjeta
AU  - Benítez, Rita
AU  - Radojković, Dragan
AU  - Lukić, Miloš
AU  - Gogić, Marija
AU  - Muñoz, María
AU  - Fontanesi, Luca
AU  - Óvilo, Cristina
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5827
AB  - This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.
PB  - MDPI AG
T2  - Animals
T1  - Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs
EP  - 24
IS  - 3
SP  - 1
VL  - 11
DO  - 10.3390/ani11030844
ER  - 

@article{
author = "Núñez, Yolanda and Radović, Čedomir and Savić, Radomir and García-Casco, Juan M. and Čandek-Potokar, Marjeta and Benítez, Rita and Radojković, Dragan and Lukić, Miloš and Gogić, Marija and Muñoz, María and Fontanesi, Luca and Óvilo, Cristina",
year = "2021",
abstract = "This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.",
publisher = "MDPI AG",
journal = "Animals",
title = "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs",
pages = "24-1",
number = "3",
volume = "11",
doi = "10.3390/ani11030844"
}

Núñez, Y., Radović, Č., Savić, R., García-Casco, J. M., Čandek-Potokar, M., Benítez, R., Radojković, D., Lukić, M., Gogić, M., Muñoz, M., Fontanesi, L.,& Óvilo, C.. (2021). Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals
MDPI AG., 11(3), 1-24.
https://doi.org/10.3390/ani11030844

Núñez Y, Radović Č, Savić R, García-Casco JM, Čandek-Potokar M, Benítez R, Radojković D, Lukić M, Gogić M, Muñoz M, Fontanesi L, Óvilo C. Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals. 2021;11(3):1-24.
doi:10.3390/ani11030844 .

Núñez, Yolanda, Radović, Čedomir, Savić, Radomir, García-Casco, Juan M., Čandek-Potokar, Marjeta, Benítez, Rita, Radojković, Dragan, Lukić, Miloš, Gogić, Marija, Muñoz, María, Fontanesi, Luca, Óvilo, Cristina, "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs" in Animals, 11, no. 3 (2021):1-24,
https://doi.org/10.3390/ani11030844 . .

TY  - JOUR
AU  - Schiavo, G.
AU  - Bovo, S.
AU  - Munoz, M.
AU  - Ribani, A.
AU  - Alves, E.
AU  - Araujo, J. P.
AU  - Bozzi, R.
AU  - Čandek-Potokar, M.
AU  - Charneca, R.
AU  - Fernandez, A. I.
AU  - Gallo, M.
AU  - Garcia, F.
AU  - Karolyi, D.
AU  - Kušec, G.
AU  - Martins, J. M.
AU  - Mercat, M.-J.
AU  - Nunez, Y.
AU  - Quintanilla, R.
AU  - Radović, C.
AU  - Razmaite, V.
AU  - Riquet, J.
AU  - Savić, R.
AU  - Usai, G.
AU  - Utzeri, V. J.
AU  - Zimmer, C.
AU  - Ovilo, C.
AU  - Fontanesi, L.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5804
AB  - ROHs are long stretches of DNA homozygous at each polymorphic position. The proportion of
genome covered by ROHs and their length are indicators of the level and origin of inbreeding.
Frequent commonROHs withinthe samepopulationdefineROHislandsand indicatehotspots of
selection. In this work, we investigated ROHs in a total of 1131 pigs from20 European local pig
breeds and in three cosmopolitan breeds, genotyped with the GGP PorcineHDGenomic Profiler.
PLINK software was used to identify ROHs. Size classes and genomic inbreeding parameters were
evaluated. ROH islands were defined by evaluating different thresholds of homozygous SNP
frequency. A functional overview of breed-specific ROH islands was obtained via overrepresentation
analyses of GO biological processes. Mora Romagnola and Turopolje breeds had
the largest proportions of genome covered with ROH (~1003 and ~955 Mb respectively),
whereas Nero Siciliano and Sarda breeds had the lowest proportions (~207 and 247 Mb
respectively). The highest proportion of long ROH (>16 Mb) was in Apulo-Calabrese, Mora
Romagnola and Casertana. The largest number of ROH islands was identified in the Italian
Landrace (n=32), Cinta Senese (n=26) and LithuanianWhite Old Type (n=22) breeds. Several
ROH islands were in regions encompassing genes known to affect morphological traits.
Comparative ROH structure analysis among breeds indicated the similar genetic structure of
local breeds across Europe. This study contributed to understanding of the genetic history of
PB  - Blackwell Publishing Ltd
T2  - Animal Genetics
T1  - Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds
DO  - 10.1111/age.13045
ER  - 

@article{
author = "Schiavo, G. and Bovo, S. and Munoz, M. and Ribani, A. and Alves, E. and Araujo, J. P. and Bozzi, R. and Čandek-Potokar, M. and Charneca, R. and Fernandez, A. I. and Gallo, M. and Garcia, F. and Karolyi, D. and Kušec, G. and Martins, J. M. and Mercat, M.-J. and Nunez, Y. and Quintanilla, R. and Radović, C. and Razmaite, V. and Riquet, J. and Savić, R. and Usai, G. and Utzeri, V. J. and Zimmer, C. and Ovilo, C. and Fontanesi, L.",
year = "2021",
abstract = "ROHs are long stretches of DNA homozygous at each polymorphic position. The proportion of
genome covered by ROHs and their length are indicators of the level and origin of inbreeding.
Frequent commonROHs withinthe samepopulationdefineROHislandsand indicatehotspots of
selection. In this work, we investigated ROHs in a total of 1131 pigs from20 European local pig
breeds and in three cosmopolitan breeds, genotyped with the GGP PorcineHDGenomic Profiler.
PLINK software was used to identify ROHs. Size classes and genomic inbreeding parameters were
evaluated. ROH islands were defined by evaluating different thresholds of homozygous SNP
frequency. A functional overview of breed-specific ROH islands was obtained via overrepresentation
analyses of GO biological processes. Mora Romagnola and Turopolje breeds had
the largest proportions of genome covered with ROH (~1003 and ~955 Mb respectively),
whereas Nero Siciliano and Sarda breeds had the lowest proportions (~207 and 247 Mb
respectively). The highest proportion of long ROH (>16 Mb) was in Apulo-Calabrese, Mora
Romagnola and Casertana. The largest number of ROH islands was identified in the Italian
Landrace (n=32), Cinta Senese (n=26) and LithuanianWhite Old Type (n=22) breeds. Several
ROH islands were in regions encompassing genes known to affect morphological traits.
Comparative ROH structure analysis among breeds indicated the similar genetic structure of
local breeds across Europe. This study contributed to understanding of the genetic history of",
publisher = "Blackwell Publishing Ltd",
journal = "Animal Genetics",
title = "Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds",
doi = "10.1111/age.13045"
}

Schiavo, G., Bovo, S., Munoz, M., Ribani, A., Alves, E., Araujo, J. P., Bozzi, R., Čandek-Potokar, M., Charneca, R., Fernandez, A. I., Gallo, M., Garcia, F., Karolyi, D., Kušec, G., Martins, J. M., Mercat, M.-J., Nunez, Y., Quintanilla, R., Radović, C., Razmaite, V., Riquet, J., Savić, R., Usai, G., Utzeri, V. J., Zimmer, C., Ovilo, C.,& Fontanesi, L.. (2021). Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds. in Animal Genetics
Blackwell Publishing Ltd..
https://doi.org/10.1111/age.13045

Schiavo G, Bovo S, Munoz M, Ribani A, Alves E, Araujo JP, Bozzi R, Čandek-Potokar M, Charneca R, Fernandez AI, Gallo M, Garcia F, Karolyi D, Kušec G, Martins JM, Mercat M, Nunez Y, Quintanilla R, Radović C, Razmaite V, Riquet J, Savić R, Usai G, Utzeri VJ, Zimmer C, Ovilo C, Fontanesi L. Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds. in Animal Genetics. 2021;.
doi:10.1111/age.13045 .

Schiavo, G., Bovo, S., Munoz, M., Ribani, A., Alves, E., Araujo, J. P., Bozzi, R., Čandek-Potokar, M., Charneca, R., Fernandez, A. I., Gallo, M., Garcia, F., Karolyi, D., Kušec, G., Martins, J. M., Mercat, M.-J., Nunez, Y., Quintanilla, R., Radović, C., Razmaite, V., Riquet, J., Savić, R., Usai, G., Utzeri, V. J., Zimmer, C., Ovilo, C., Fontanesi, L., "Runs of homozygosity provide a genome landscape picture of inbreeding and genetic history of European autochthonous and commercial pig breeds" in Animal Genetics (2021),
https://doi.org/10.1111/age.13045 . .

TY  - JOUR
AU  - Bovo, Samuele
AU  - Schiavo, Giuseppina
AU  - Ribani, Anisa
AU  - Utzeri, Valerio J.
AU  - Taurisano, Valeria
AU  - Ballan, Mohamad
AU  - Muñoz, Maria
AU  - Alves, Estefania
AU  - Araujo, Jose P.
AU  - Bozzi, Riccardo
AU  - Charneca, Rui
AU  - Di Palma, Federica
AU  - Djurkin Kušec, Ivona
AU  - Etherington, Graham
AU  - Fernandez, Ana I.
AU  - García, Fabián
AU  - García‑Casco, Juan
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Martins, José Manuel
AU  - Mercat, Marie‑José
AU  - Núñez, Yolanda
AU  - Quintanilla, Raquel
AU  - Radović, Čedomir
AU  - Razmaite, Violeta
AU  - Riquet, Juliette
AU  - Savić, Radomir
AU  - Škrlep, Martin
AU  - Usai, Graziano
AU  - Zimmer, Christoph
AU  - Ovilo, Cristina
AU  - Fontanesi, Luca
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5807
AB  - Coronaviruses silently circulate in human and animal populations, causing mild to severe diseases. Therefore, livestock are important components of a “One Health” perspective aimed to control these viral infections. However, at present there is no example that considers pig genetic resources in this context. In this study, we investigated the variability of four genes (ACE2, ANPEP and DPP4 encoding for host receptors of the viral spike proteins and TMPRSS2 encoding for a host proteinase) in 23 European (19 autochthonous and three commercial breeds and one wild boar population) and two Asian Sus scrofa populations. A total of 2229 variants were identified in the four candidate genes: 26% of them were not previously described; 29 variants affected the protein sequence and might potentially interact with the infection mechanisms. The results coming from this work are a first step towards a “One Health” perspective that should consider conservation programs of pig genetic resources with twofold objectives: (i) genetic resources could be reservoirs of host gene variability useful to design selection programs to increase resistance to coronaviruses; (ii) the described variability in genes involved in coronavirus infections across many different pig populations might be part of a risk assessment including pig genetic resources
PB  - Nature Research
T2  - Scientific Reports
T1  - Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources
IS  - 1
SP  - 3359
VL  - 11
DO  - 10.1038/s41598-021-82956-0
ER  - 

@article{
author = "Bovo, Samuele and Schiavo, Giuseppina and Ribani, Anisa and Utzeri, Valerio J. and Taurisano, Valeria and Ballan, Mohamad and Muñoz, Maria and Alves, Estefania and Araujo, Jose P. and Bozzi, Riccardo and Charneca, Rui and Di Palma, Federica and Djurkin Kušec, Ivona and Etherington, Graham and Fernandez, Ana I. and García, Fabián and García‑Casco, Juan and Karolyi, Danijel and Gallo, Maurizio and Martins, José Manuel and Mercat, Marie‑José and Núñez, Yolanda and Quintanilla, Raquel and Radović, Čedomir and Razmaite, Violeta and Riquet, Juliette and Savić, Radomir and Škrlep, Martin and Usai, Graziano and Zimmer, Christoph and Ovilo, Cristina and Fontanesi, Luca",
year = "2021",
abstract = "Coronaviruses silently circulate in human and animal populations, causing mild to severe diseases. Therefore, livestock are important components of a “One Health” perspective aimed to control these viral infections. However, at present there is no example that considers pig genetic resources in this context. In this study, we investigated the variability of four genes (ACE2, ANPEP and DPP4 encoding for host receptors of the viral spike proteins and TMPRSS2 encoding for a host proteinase) in 23 European (19 autochthonous and three commercial breeds and one wild boar population) and two Asian Sus scrofa populations. A total of 2229 variants were identified in the four candidate genes: 26% of them were not previously described; 29 variants affected the protein sequence and might potentially interact with the infection mechanisms. The results coming from this work are a first step towards a “One Health” perspective that should consider conservation programs of pig genetic resources with twofold objectives: (i) genetic resources could be reservoirs of host gene variability useful to design selection programs to increase resistance to coronaviruses; (ii) the described variability in genes involved in coronavirus infections across many different pig populations might be part of a risk assessment including pig genetic resources",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources",
number = "1",
pages = "3359",
volume = "11",
doi = "10.1038/s41598-021-82956-0"
}

Bovo, S., Schiavo, G., Ribani, A., Utzeri, V. J., Taurisano, V., Ballan, M., Muñoz, M., Alves, E., Araujo, J. P., Bozzi, R., Charneca, R., Di Palma, F., Djurkin Kušec, I., Etherington, G., Fernandez, A. I., García, F., García‑Casco, J., Karolyi, D., Gallo, M., Martins, J. M., Mercat, M., Núñez, Y., Quintanilla, R., Radović, Č., Razmaite, V., Riquet, J., Savić, R., Škrlep, M., Usai, G., Zimmer, C., Ovilo, C.,& Fontanesi, L.. (2021). Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources. in Scientific Reports
Nature Research., 11(1), 3359.
https://doi.org/10.1038/s41598-021-82956-0

Bovo S, Schiavo G, Ribani A, Utzeri VJ, Taurisano V, Ballan M, Muñoz M, Alves E, Araujo JP, Bozzi R, Charneca R, Di Palma F, Djurkin Kušec I, Etherington G, Fernandez AI, García F, García‑Casco J, Karolyi D, Gallo M, Martins JM, Mercat M, Núñez Y, Quintanilla R, Radović Č, Razmaite V, Riquet J, Savić R, Škrlep M, Usai G, Zimmer C, Ovilo C, Fontanesi L. Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources. in Scientific Reports. 2021;11(1):3359.
doi:10.1038/s41598-021-82956-0 .

Bovo, Samuele, Schiavo, Giuseppina, Ribani, Anisa, Utzeri, Valerio J., Taurisano, Valeria, Ballan, Mohamad, Muñoz, Maria, Alves, Estefania, Araujo, Jose P., Bozzi, Riccardo, Charneca, Rui, Di Palma, Federica, Djurkin Kušec, Ivona, Etherington, Graham, Fernandez, Ana I., García, Fabián, García‑Casco, Juan, Karolyi, Danijel, Gallo, Maurizio, Martins, José Manuel, Mercat, Marie‑José, Núñez, Yolanda, Quintanilla, Raquel, Radović, Čedomir, Razmaite, Violeta, Riquet, Juliette, Savić, Radomir, Škrlep, Martin, Usai, Graziano, Zimmer, Christoph, Ovilo, Cristina, Fontanesi, Luca, "Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources" in Scientific Reports, 11, no. 1 (2021):3359,
https://doi.org/10.1038/s41598-021-82956-0 . .

TY  - JOUR
AU  - Bovo, Samuele
AU  - Ribani, Anisa
AU  - Munoz, Maria
AU  - Alves, Estefania
AU  - Araujo, Jose P.
AU  - Bozzi, Riccardo
AU  - Čandek-Potokar, Marjeta
AU  - Charneca, Rui
AU  - Di Palma, Federica
AU  - Etherington, Graham
AU  - Fernandez, Ana I
AU  - Garcia, Fabian
AU  - Garcia-Casco, Juan
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Margeta, Vladimir
AU  - Martins, Jose M.
AU  - Mercat, Marie J.
AU  - Moscatelli, Giulia
AU  - Nunez, Yolanda
AU  - Quintanilla, Raquel
AU  - Radović, Čedomir
AU  - Razmaite, Violeta
AU  - Riquet, Juliette
AU  - Savić, Radomir
AU  - Schiavo, Giuseppina
AU  - Usai, Graziano
AU  - Utzeri, Valerio J.
AU  - Zimmer, Christoph
AU  - Ovilo, Cristina
AU  - Fontanesi, Luca
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5252
AB  - BACKGROUND:  Natural and artificial directional selection in cosmopolitan and autochthonous pig breeds and wild boars have shaped their genomes and resulted in a reservoir of animal genetic diversity. Signatures of selection are the result of these selection events that have contributed to the adaptation of breeds to different environments and production systems. In this study, we analysed the genome variability of 19 European autochthonous pig breeds (Alentejana, Bisara, Majorcan Black, Basque, Gascon, Apulo-Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano, Sarda, Krskopolje pig, Black Slavonian, Turopolje, Moravka, Swallow-Bellied Mangalitsa, Schwabisch-Hallisches Schwein, Lithuanian indigenous wattle and Lithuanian White old type) from nine countries, three European commercial breeds (Italian Large White, Italian Landrace and Italian Duroc), and European wild boars, by mining whole-genome sequencing data obtained by using a DNA-pool sequencing approach. Signatures of selection were identified by using a single-breed approach with two statistics [within-breed pooled heterozygosity (H-P) and fixation index (F-ST)] and group-based F(ST)approaches, which compare groups of breeds defined according to external traits and use/specialization/type. Results We detected more than 22 million single nucleotide polymorphisms (SNPs) across the 23 compared populations and identified 359 chromosome regions showing signatures of selection. These regions harbour genes that are already known or new genes that are under selection and relevant for the domestication process in this species, and that affect several morphological and physiological traits (e.g. coat colours and patterns, body size, number of vertebrae and teats, ear size and conformation, reproductive traits, growth and fat deposition traits). Wild boar related signatures of selection were detected across all the genome of several autochthonous breeds, which suggests that crossbreeding (accidental or deliberate) occurred with wild boars. Conclusions Our findings provide a catalogue of genetic variants of many European pig populations and identify genome regions that can explain, at least in part, the phenotypic diversity of these genetic resources.
PB  - Bmc, London
T2  - Genetics Selection Evolution
T1  - Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems
IS  - 1
VL  - 52
DO  - 10.1186/s12711-020-00553-7
ER  - 

@article{
author = "Bovo, Samuele and Ribani, Anisa and Munoz, Maria and Alves, Estefania and Araujo, Jose P. and Bozzi, Riccardo and Čandek-Potokar, Marjeta and Charneca, Rui and Di Palma, Federica and Etherington, Graham and Fernandez, Ana I and Garcia, Fabian and Garcia-Casco, Juan and Karolyi, Danijel and Gallo, Maurizio and Margeta, Vladimir and Martins, Jose M. and Mercat, Marie J. and Moscatelli, Giulia and Nunez, Yolanda and Quintanilla, Raquel and Radović, Čedomir and Razmaite, Violeta and Riquet, Juliette and Savić, Radomir and Schiavo, Giuseppina and Usai, Graziano and Utzeri, Valerio J. and Zimmer, Christoph and Ovilo, Cristina and Fontanesi, Luca",
year = "2020",
abstract = "BACKGROUND:  Natural and artificial directional selection in cosmopolitan and autochthonous pig breeds and wild boars have shaped their genomes and resulted in a reservoir of animal genetic diversity. Signatures of selection are the result of these selection events that have contributed to the adaptation of breeds to different environments and production systems. In this study, we analysed the genome variability of 19 European autochthonous pig breeds (Alentejana, Bisara, Majorcan Black, Basque, Gascon, Apulo-Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano, Sarda, Krskopolje pig, Black Slavonian, Turopolje, Moravka, Swallow-Bellied Mangalitsa, Schwabisch-Hallisches Schwein, Lithuanian indigenous wattle and Lithuanian White old type) from nine countries, three European commercial breeds (Italian Large White, Italian Landrace and Italian Duroc), and European wild boars, by mining whole-genome sequencing data obtained by using a DNA-pool sequencing approach. Signatures of selection were identified by using a single-breed approach with two statistics [within-breed pooled heterozygosity (H-P) and fixation index (F-ST)] and group-based F(ST)approaches, which compare groups of breeds defined according to external traits and use/specialization/type. Results We detected more than 22 million single nucleotide polymorphisms (SNPs) across the 23 compared populations and identified 359 chromosome regions showing signatures of selection. These regions harbour genes that are already known or new genes that are under selection and relevant for the domestication process in this species, and that affect several morphological and physiological traits (e.g. coat colours and patterns, body size, number of vertebrae and teats, ear size and conformation, reproductive traits, growth and fat deposition traits). Wild boar related signatures of selection were detected across all the genome of several autochthonous breeds, which suggests that crossbreeding (accidental or deliberate) occurred with wild boars. Conclusions Our findings provide a catalogue of genetic variants of many European pig populations and identify genome regions that can explain, at least in part, the phenotypic diversity of these genetic resources.",
publisher = "Bmc, London",
journal = "Genetics Selection Evolution",
title = "Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems",
number = "1",
volume = "52",
doi = "10.1186/s12711-020-00553-7"
}

Bovo, S., Ribani, A., Munoz, M., Alves, E., Araujo, J. P., Bozzi, R., Čandek-Potokar, M., Charneca, R., Di Palma, F., Etherington, G., Fernandez, A. I., Garcia, F., Garcia-Casco, J., Karolyi, D., Gallo, M., Margeta, V., Martins, J. M., Mercat, M. J., Moscatelli, G., Nunez, Y., Quintanilla, R., Radović, Č., Razmaite, V., Riquet, J., Savić, R., Schiavo, G., Usai, G., Utzeri, V. J., Zimmer, C., Ovilo, C.,& Fontanesi, L.. (2020). Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems. in Genetics Selection Evolution
Bmc, London., 52(1).
https://doi.org/10.1186/s12711-020-00553-7

Bovo S, Ribani A, Munoz M, Alves E, Araujo JP, Bozzi R, Čandek-Potokar M, Charneca R, Di Palma F, Etherington G, Fernandez AI, Garcia F, Garcia-Casco J, Karolyi D, Gallo M, Margeta V, Martins JM, Mercat MJ, Moscatelli G, Nunez Y, Quintanilla R, Radović Č, Razmaite V, Riquet J, Savić R, Schiavo G, Usai G, Utzeri VJ, Zimmer C, Ovilo C, Fontanesi L. Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems. in Genetics Selection Evolution. 2020;52(1).
doi:10.1186/s12711-020-00553-7 .

Bovo, Samuele, Ribani, Anisa, Munoz, Maria, Alves, Estefania, Araujo, Jose P., Bozzi, Riccardo, Čandek-Potokar, Marjeta, Charneca, Rui, Di Palma, Federica, Etherington, Graham, Fernandez, Ana I, Garcia, Fabian, Garcia-Casco, Juan, Karolyi, Danijel, Gallo, Maurizio, Margeta, Vladimir, Martins, Jose M., Mercat, Marie J., Moscatelli, Giulia, Nunez, Yolanda, Quintanilla, Raquel, Radović, Čedomir, Razmaite, Violeta, Riquet, Juliette, Savić, Radomir, Schiavo, Giuseppina, Usai, Graziano, Utzeri, Valerio J., Zimmer, Christoph, Ovilo, Cristina, Fontanesi, Luca, "Whole-genome sequencing of European autochthonous and commercial pig breeds allows the detection of signatures of selection for adaptation of genetic resources to different breeding and production systems" in Genetics Selection Evolution, 52, no. 1 (2020),
https://doi.org/10.1186/s12711-020-00553-7 . .

TY  - JOUR
AU  - Bovo, Samuele
AU  - Ribani, A.
AU  - Munoz, Maria
AU  - Alves, Estefania
AU  - Araujo, Jose P.
AU  - Bozzi, Riccardo
AU  - Charneca, R.
AU  - Di Palma, F.
AU  - Etherington, G.
AU  - Fernandez, Ana I
AU  - Garcia, Fabian
AU  - Garcia-Casco, Juan
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Gvozdanović, K.
AU  - Martins, Jose M.
AU  - Mercat, Marie J.
AU  - Nunez, Yolanda
AU  - Quintanilla, Raquel
AU  - Razmaite, Violeta
AU  - Riquet, Juliette
AU  - Savić, Radomir
AU  - Schiavo, Giuseppina
AU  - Skrlep, Martin
AU  - Usai, Graziano
AU  - Utzeri, Valerio J.
AU  - Zimmer, Christoph
AU  - Ovilo, Cristina
AU  - Fontanesi, Luca
AU  - Radović, Čedomir
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5349
AB  - In this study, we identified copy number variants (CNVs) in 19 European autochthonous pig breeds and in two commercial breeds (Italian Large White and Italian Duroc) that represent important genetic resources for this species. The genome of 725 pigs was sequenced using a breed-specific DNA pooling approach (30-35 animals per pool) obtaining an average depth per pool of 42x. This approach maximised CNV discovery as well as the related copy number states characterising, on average, the analysed breeds. By mining more than 17.5 billion reads, we identified a total of 9592 CNVs (similar to 683 CNVs per breed) and 3710 CNV regions (CNVRs; 1.15% of the reference pig genome), with an average of 77 CNVRs per breed that were considered as private. A few CNVRs were analysed in more detail, together with other information derived from sequencing data. For example, the CNVR encompassing the KIT gene was associated with coat colour phenotypes in the analysed breeds, confirming the role of the multiple copies in determining breed-specific coat colours. The CNVR covering the MSRB3 gene was associated with ear size in most breeds. The CNVRs affecting the ELOVL6 and ZNF622 genes were private features observed in the Lithuanian Indigenous Wattle and in the Turopolje pig breeds respectively. Overall, the genome variability unravelled here can explain part of the genetic diversity among breeds and might contribute to explain their origin, history and adaptation to a variety of production systems.
PB  - Wiley, Hoboken
T2  - Animal Genetics
T1  - Genome-wide detection of copy number variants in European autochthonous and commercial pig breeds by whole-genome sequencing of DNA pools identified breed-characterising copy number states
EP  - 556
IS  - 4
SP  - 541
VL  - 51
DO  - 10.1111/age.12954
ER  - 

@article{
author = "Bovo, Samuele and Ribani, A. and Munoz, Maria and Alves, Estefania and Araujo, Jose P. and Bozzi, Riccardo and Charneca, R. and Di Palma, F. and Etherington, G. and Fernandez, Ana I and Garcia, Fabian and Garcia-Casco, Juan and Karolyi, Danijel and Gallo, Maurizio and Gvozdanović, K. and Martins, Jose M. and Mercat, Marie J. and Nunez, Yolanda and Quintanilla, Raquel and Razmaite, Violeta and Riquet, Juliette and Savić, Radomir and Schiavo, Giuseppina and Skrlep, Martin and Usai, Graziano and Utzeri, Valerio J. and Zimmer, Christoph and Ovilo, Cristina and Fontanesi, Luca and Radović, Čedomir",
year = "2020",
abstract = "In this study, we identified copy number variants (CNVs) in 19 European autochthonous pig breeds and in two commercial breeds (Italian Large White and Italian Duroc) that represent important genetic resources for this species. The genome of 725 pigs was sequenced using a breed-specific DNA pooling approach (30-35 animals per pool) obtaining an average depth per pool of 42x. This approach maximised CNV discovery as well as the related copy number states characterising, on average, the analysed breeds. By mining more than 17.5 billion reads, we identified a total of 9592 CNVs (similar to 683 CNVs per breed) and 3710 CNV regions (CNVRs; 1.15% of the reference pig genome), with an average of 77 CNVRs per breed that were considered as private. A few CNVRs were analysed in more detail, together with other information derived from sequencing data. For example, the CNVR encompassing the KIT gene was associated with coat colour phenotypes in the analysed breeds, confirming the role of the multiple copies in determining breed-specific coat colours. The CNVR covering the MSRB3 gene was associated with ear size in most breeds. The CNVRs affecting the ELOVL6 and ZNF622 genes were private features observed in the Lithuanian Indigenous Wattle and in the Turopolje pig breeds respectively. Overall, the genome variability unravelled here can explain part of the genetic diversity among breeds and might contribute to explain their origin, history and adaptation to a variety of production systems.",
publisher = "Wiley, Hoboken",
journal = "Animal Genetics",
title = "Genome-wide detection of copy number variants in European autochthonous and commercial pig breeds by whole-genome sequencing of DNA pools identified breed-characterising copy number states",
pages = "556-541",
number = "4",
volume = "51",
doi = "10.1111/age.12954"
}

Bovo, S., Ribani, A., Munoz, M., Alves, E., Araujo, J. P., Bozzi, R., Charneca, R., Di Palma, F., Etherington, G., Fernandez, A. I., Garcia, F., Garcia-Casco, J., Karolyi, D., Gallo, M., Gvozdanović, K., Martins, J. M., Mercat, M. J., Nunez, Y., Quintanilla, R., Razmaite, V., Riquet, J., Savić, R., Schiavo, G., Skrlep, M., Usai, G., Utzeri, V. J., Zimmer, C., Ovilo, C., Fontanesi, L.,& Radović, Č.. (2020). Genome-wide detection of copy number variants in European autochthonous and commercial pig breeds by whole-genome sequencing of DNA pools identified breed-characterising copy number states. in Animal Genetics
Wiley, Hoboken., 51(4), 541-556.
https://doi.org/10.1111/age.12954

Bovo S, Ribani A, Munoz M, Alves E, Araujo JP, Bozzi R, Charneca R, Di Palma F, Etherington G, Fernandez AI, Garcia F, Garcia-Casco J, Karolyi D, Gallo M, Gvozdanović K, Martins JM, Mercat MJ, Nunez Y, Quintanilla R, Razmaite V, Riquet J, Savić R, Schiavo G, Skrlep M, Usai G, Utzeri VJ, Zimmer C, Ovilo C, Fontanesi L, Radović Č. Genome-wide detection of copy number variants in European autochthonous and commercial pig breeds by whole-genome sequencing of DNA pools identified breed-characterising copy number states. in Animal Genetics. 2020;51(4):541-556.
doi:10.1111/age.12954 .

Bovo, Samuele, Ribani, A., Munoz, Maria, Alves, Estefania, Araujo, Jose P., Bozzi, Riccardo, Charneca, R., Di Palma, F., Etherington, G., Fernandez, Ana I, Garcia, Fabian, Garcia-Casco, Juan, Karolyi, Danijel, Gallo, Maurizio, Gvozdanović, K., Martins, Jose M., Mercat, Marie J., Nunez, Yolanda, Quintanilla, Raquel, Razmaite, Violeta, Riquet, Juliette, Savić, Radomir, Schiavo, Giuseppina, Skrlep, Martin, Usai, Graziano, Utzeri, Valerio J., Zimmer, Christoph, Ovilo, Cristina, Fontanesi, Luca, Radović, Čedomir, "Genome-wide detection of copy number variants in European autochthonous and commercial pig breeds by whole-genome sequencing of DNA pools identified breed-characterising copy number states" in Animal Genetics, 51, no. 4 (2020):541-556,
https://doi.org/10.1111/age.12954 . .

TY  - JOUR
AU  - Ribani, A.
AU  - Utzeri, Valerio J.
AU  - Geraci, Claudia
AU  - Tinarelli, S.
AU  - Djan, M.
AU  - Velicković, N.
AU  - Doneva, R.
AU  - Dall'Olio, S.
AU  - Costa, L.Nanni
AU  - Schiavo, Giuseppina
AU  - Bovo, Samuele
AU  - Usai, Graziano
AU  - Gallo, Maurizio
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - Karolyi, Danijel
AU  - Salajpal, K.
AU  - Gvozdanović, K.
AU  - Djurkin-Kusec, Ivona
AU  - Skrlep, Martin
AU  - Čandek-Potokar, Marjeta
AU  - Ovilo, Cristina
AU  - Fontanesi, Luca
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5109
AB  - Autochthonous pig breeds are usually reared in extensive or semi-extensive production systems that might facilitate contact with wild boars and, thus, reciprocal genetic exchanges. In this study, we analysed variants in the melanocortin 1 receptor (MC1R) gene (which cause different coat colour phenotypes) and in the nuclear receptor subfamily 6 group A member 1 (NR6A1) gene (associated with increased vertebral number) in 712 pigs of 12 local pig breeds raised in Italy (Apulo-Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano and Sarda) and south-eastern European countries (Krskopolje from Slovenia, Black Slavonian and Turopolje from Croatia, Mangalitsa and Moravka from Serbia and East Balkan Swine from Bulgaria) and compared the data with the genetic variability at these loci investigated in 229 wild boars from populations spread in the same macro-geographic areas. None of the autochthonous pig breeds or wild boar populations were fixed for one allele at both loci. Domestic and wild-type alleles at these two genes were present in both domestic and wild populations. Findings of the distribution of MC1R alleles might be useful for tracing back the complex genetic history of autochthonous breeds. Altogether, these results indirectly demonstrate that bidirectional introgression of wild and domestic alleles is derived and affected by the human and naturally driven evolutionary forces that are shaping the Sus scrofa genome: autochthonous breeds are experiencing a sort of 'de-domestication' process, and wild resources are challenged by a 'domestication' drift. Both need to be further investigated and managed.
PB  - Wiley, Hoboken
T2  - Animal Genetics
T1  - Signatures of de-domestication in autochthonous pig breeds and of domestication in wild boar populations from MC1R and NR6A1 allele distribution
EP  - 171
IS  - 2
SP  - 166
VL  - 50
DO  - 10.1111/age.12771
ER  - 

@article{
author = "Ribani, A. and Utzeri, Valerio J. and Geraci, Claudia and Tinarelli, S. and Djan, M. and Velicković, N. and Doneva, R. and Dall'Olio, S. and Costa, L.Nanni and Schiavo, Giuseppina and Bovo, Samuele and Usai, Graziano and Gallo, Maurizio and Radović, Čedomir and Savić, Radomir and Karolyi, Danijel and Salajpal, K. and Gvozdanović, K. and Djurkin-Kusec, Ivona and Skrlep, Martin and Čandek-Potokar, Marjeta and Ovilo, Cristina and Fontanesi, Luca",
year = "2019",
abstract = "Autochthonous pig breeds are usually reared in extensive or semi-extensive production systems that might facilitate contact with wild boars and, thus, reciprocal genetic exchanges. In this study, we analysed variants in the melanocortin 1 receptor (MC1R) gene (which cause different coat colour phenotypes) and in the nuclear receptor subfamily 6 group A member 1 (NR6A1) gene (associated with increased vertebral number) in 712 pigs of 12 local pig breeds raised in Italy (Apulo-Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano and Sarda) and south-eastern European countries (Krskopolje from Slovenia, Black Slavonian and Turopolje from Croatia, Mangalitsa and Moravka from Serbia and East Balkan Swine from Bulgaria) and compared the data with the genetic variability at these loci investigated in 229 wild boars from populations spread in the same macro-geographic areas. None of the autochthonous pig breeds or wild boar populations were fixed for one allele at both loci. Domestic and wild-type alleles at these two genes were present in both domestic and wild populations. Findings of the distribution of MC1R alleles might be useful for tracing back the complex genetic history of autochthonous breeds. Altogether, these results indirectly demonstrate that bidirectional introgression of wild and domestic alleles is derived and affected by the human and naturally driven evolutionary forces that are shaping the Sus scrofa genome: autochthonous breeds are experiencing a sort of 'de-domestication' process, and wild resources are challenged by a 'domestication' drift. Both need to be further investigated and managed.",
publisher = "Wiley, Hoboken",
journal = "Animal Genetics",
title = "Signatures of de-domestication in autochthonous pig breeds and of domestication in wild boar populations from MC1R and NR6A1 allele distribution",
pages = "171-166",
number = "2",
volume = "50",
doi = "10.1111/age.12771"
}

Ribani, A., Utzeri, V. J., Geraci, C., Tinarelli, S., Djan, M., Velicković, N., Doneva, R., Dall'Olio, S., Costa, L.Nanni, Schiavo, G., Bovo, S., Usai, G., Gallo, M., Radović, Č., Savić, R., Karolyi, D., Salajpal, K., Gvozdanović, K., Djurkin-Kusec, I., Skrlep, M., Čandek-Potokar, M., Ovilo, C.,& Fontanesi, L.. (2019). Signatures of de-domestication in autochthonous pig breeds and of domestication in wild boar populations from MC1R and NR6A1 allele distribution. in Animal Genetics
Wiley, Hoboken., 50(2), 166-171.
https://doi.org/10.1111/age.12771

Ribani A, Utzeri VJ, Geraci C, Tinarelli S, Djan M, Velicković N, Doneva R, Dall'Olio S, Costa L, Schiavo G, Bovo S, Usai G, Gallo M, Radović Č, Savić R, Karolyi D, Salajpal K, Gvozdanović K, Djurkin-Kusec I, Skrlep M, Čandek-Potokar M, Ovilo C, Fontanesi L. Signatures of de-domestication in autochthonous pig breeds and of domestication in wild boar populations from MC1R and NR6A1 allele distribution. in Animal Genetics. 2019;50(2):166-171.
doi:10.1111/age.12771 .

Ribani, A., Utzeri, Valerio J., Geraci, Claudia, Tinarelli, S., Djan, M., Velicković, N., Doneva, R., Dall'Olio, S., Costa, L.Nanni, Schiavo, Giuseppina, Bovo, Samuele, Usai, Graziano, Gallo, Maurizio, Radović, Čedomir, Savić, Radomir, Karolyi, Danijel, Salajpal, K., Gvozdanović, K., Djurkin-Kusec, Ivona, Skrlep, Martin, Čandek-Potokar, Marjeta, Ovilo, Cristina, Fontanesi, Luca, "Signatures of de-domestication in autochthonous pig breeds and of domestication in wild boar populations from MC1R and NR6A1 allele distribution" in Animal Genetics, 50, no. 2 (2019):166-171,
https://doi.org/10.1111/age.12771 . .

TY  - JOUR
AU  - Gogić, Marija
AU  - Radović, Čedomir
AU  - Čandek-Potokar, Marjeta
AU  - Petrović, Milica
AU  - Radojković, Dragan
AU  - Parunović, Nenad
AU  - Savić, Radomir
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4998
AB  - The objective of this research was to study the effect of immunocastration on the morphometric characteristics of sex glands in male Swallow-bellied Mangalitsa pigs. The study included 24 animals (13 entire and 11 immunocastrated males). The research focused on the following traits: age at slaughter (days), live weight at slaughter (kg), growth rate (kg day(-1)), and morphometric testicle traits (volume, cm(3); weight, g; length, width, thickness, and circumference, mm). The efficacy of immunocastration was determined based on androstenone and skatole levels in adipose tissue. The group of immunocastrated animals had higher body weight at slaughter (+18.8 kg) due to higher average daily live gain (+87 g). Immunocastration resulted in reduced morphometric measures of testes. The volumes and weight of testes/epididymes were reduced by 28.9-54.4 and 32.1-53.3%, respectively. Testis length and other size-related traits were significantly reduced by 17.4-27.6%. The linear regression effect of live body weight at slaughter within the immunocastrated group on testis measurements was not significant. Within the entire group of males, the increase of live body weight at slaughter by 1 kg had an effect on the increase of volume and weight of testes/epididymes by 0.55-2.24 cm(3) and 0.52-2.28 g, respectively. Other size-related testis traits increased by 0.04-0.55 mm for each kg of body weight. Immunocastration also resulted in 79.6% decrease in the weight of accessory sex glands. The present study is the first to demonstrate that immunocastration could be a method of choice for castration of the local fatty pig breed, Mangulica (Swallow-bellied Mangalitsa).
PB  - Revista Brasileira Zootecnia Brazilian Journal Animal Sci, Vicosa-Mg
T2  - Revista Brasileira de Zootecnia-Brazilian Journal of Animal Science
T1  - Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs
VL  - 48
DO  - 10.1590/rbz4820180286
ER  - 

@article{
author = "Gogić, Marija and Radović, Čedomir and Čandek-Potokar, Marjeta and Petrović, Milica and Radojković, Dragan and Parunović, Nenad and Savić, Radomir",
year = "2019",
abstract = "The objective of this research was to study the effect of immunocastration on the morphometric characteristics of sex glands in male Swallow-bellied Mangalitsa pigs. The study included 24 animals (13 entire and 11 immunocastrated males). The research focused on the following traits: age at slaughter (days), live weight at slaughter (kg), growth rate (kg day(-1)), and morphometric testicle traits (volume, cm(3); weight, g; length, width, thickness, and circumference, mm). The efficacy of immunocastration was determined based on androstenone and skatole levels in adipose tissue. The group of immunocastrated animals had higher body weight at slaughter (+18.8 kg) due to higher average daily live gain (+87 g). Immunocastration resulted in reduced morphometric measures of testes. The volumes and weight of testes/epididymes were reduced by 28.9-54.4 and 32.1-53.3%, respectively. Testis length and other size-related traits were significantly reduced by 17.4-27.6%. The linear regression effect of live body weight at slaughter within the immunocastrated group on testis measurements was not significant. Within the entire group of males, the increase of live body weight at slaughter by 1 kg had an effect on the increase of volume and weight of testes/epididymes by 0.55-2.24 cm(3) and 0.52-2.28 g, respectively. Other size-related testis traits increased by 0.04-0.55 mm for each kg of body weight. Immunocastration also resulted in 79.6% decrease in the weight of accessory sex glands. The present study is the first to demonstrate that immunocastration could be a method of choice for castration of the local fatty pig breed, Mangulica (Swallow-bellied Mangalitsa).",
publisher = "Revista Brasileira Zootecnia Brazilian Journal Animal Sci, Vicosa-Mg",
journal = "Revista Brasileira de Zootecnia-Brazilian Journal of Animal Science",
title = "Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs",
volume = "48",
doi = "10.1590/rbz4820180286"
}

Gogić, M., Radović, Č., Čandek-Potokar, M., Petrović, M., Radojković, D., Parunović, N.,& Savić, R.. (2019). Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs. in Revista Brasileira de Zootecnia-Brazilian Journal of Animal Science
Revista Brasileira Zootecnia Brazilian Journal Animal Sci, Vicosa-Mg., 48.
https://doi.org/10.1590/rbz4820180286

Gogić M, Radović Č, Čandek-Potokar M, Petrović M, Radojković D, Parunović N, Savić R. Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs. in Revista Brasileira de Zootecnia-Brazilian Journal of Animal Science. 2019;48.
doi:10.1590/rbz4820180286 .

Gogić, Marija, Radović, Čedomir, Čandek-Potokar, Marjeta, Petrović, Milica, Radojković, Dragan, Parunović, Nenad, Savić, Radomir, "Effect of immunocastration on sex glands of male Mangulica (Swallow-bellied Mangalitsa) pigs" in Revista Brasileira de Zootecnia-Brazilian Journal of Animal Science, 48 (2019),
https://doi.org/10.1590/rbz4820180286 . .

TY  - JOUR
AU  - Munoz, Maria
AU  - Bozzi, Riccardo
AU  - Garcia-Casco, Juan
AU  - Nunez, Yolanda
AU  - Ribani, A.
AU  - Franci, O.
AU  - Garcia, Fabian
AU  - Skrlep, Martin
AU  - Schiavo, Giuseppina
AU  - Bovo, Samuele
AU  - Utzeri, Valerio J.
AU  - Charneca, R.
AU  - Martins, Jose M.
AU  - Quintanilla, Raquel
AU  - Tibau, J.
AU  - Margeta, Vladimir
AU  - Djurkin-Kusec, Ivona
AU  - Mercat, Marie J.
AU  - Riquet, Juliette
AU  - Estelle, Jordi
AU  - Zimmer, Christoph
AU  - Razmaite, Violeta
AU  - Araujo, Jose P.
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Čandek-Potokar, Marjeta
AU  - Fernandez, Ana I
AU  - Fontanesi, Luca
AU  - Ovilo, Cristina
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4915
AB  - Genetic characterization of local breeds is essential to preserve their genomic variability, to advance conservation policies and to contribute to their promotion and sustainability. Genomic diversity of twenty European local pig breeds and a small sample of Spanish wild pigs was assessed using high density SNP chips. A total of 992 DNA samples were analyzed with the GeneSeek Genomic Profiler (GGP) 70 K HD porcine genotyping chip. Genotype data was employed to compute genetic diversity, population differentiation and structure, genetic distances, linkage disequilibrium and effective population size. Our results point out several breeds, such as Turopolje, Apulo Calabrese, Casertana, Mora Romagnola and Lithuanian indigenous wattle, having the lowest genetic diversity, supported by low heterozygosity and very small effective population size, demonstrating the need of enhanced conservation strategies. Principal components analysis showed the clustering of the individuals of the same breed, with few breeds being clearly isolated from the rest. Several breeds were partially overlapped, suggesting genetic closeness, which was particularly marked in the case of Iberian and Alentejana breeds. Spanish wild boar was also narrowly related to other western populations, in agreement with recurrent admixture between wild and domestic animals. We also searched across the genome for loci under diversifying selection based on F-S(T) outlier tests. Candidate genes that may underlie differences in adaptation to specific environments and productive systems and phenotypic traits were detected in potentially selected genomic regions.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Genomic diversity, linkage disequilibrium and selection signatures in European local pig breeds assessed with a high density SNP chip
VL  - 9
DO  - 10.1038/s41598-019-49830-6
ER  - 

@article{
author = "Munoz, Maria and Bozzi, Riccardo and Garcia-Casco, Juan and Nunez, Yolanda and Ribani, A. and Franci, O. and Garcia, Fabian and Skrlep, Martin and Schiavo, Giuseppina and Bovo, Samuele and Utzeri, Valerio J. and Charneca, R. and Martins, Jose M. and Quintanilla, Raquel and Tibau, J. and Margeta, Vladimir and Djurkin-Kusec, Ivona and Mercat, Marie J. and Riquet, Juliette and Estelle, Jordi and Zimmer, Christoph and Razmaite, Violeta and Araujo, Jose P. and Radović, Čedomir and Savić, Radomir and Karolyi, Danijel and Gallo, Maurizio and Čandek-Potokar, Marjeta and Fernandez, Ana I and Fontanesi, Luca and Ovilo, Cristina",
year = "2019",
abstract = "Genetic characterization of local breeds is essential to preserve their genomic variability, to advance conservation policies and to contribute to their promotion and sustainability. Genomic diversity of twenty European local pig breeds and a small sample of Spanish wild pigs was assessed using high density SNP chips. A total of 992 DNA samples were analyzed with the GeneSeek Genomic Profiler (GGP) 70 K HD porcine genotyping chip. Genotype data was employed to compute genetic diversity, population differentiation and structure, genetic distances, linkage disequilibrium and effective population size. Our results point out several breeds, such as Turopolje, Apulo Calabrese, Casertana, Mora Romagnola and Lithuanian indigenous wattle, having the lowest genetic diversity, supported by low heterozygosity and very small effective population size, demonstrating the need of enhanced conservation strategies. Principal components analysis showed the clustering of the individuals of the same breed, with few breeds being clearly isolated from the rest. Several breeds were partially overlapped, suggesting genetic closeness, which was particularly marked in the case of Iberian and Alentejana breeds. Spanish wild boar was also narrowly related to other western populations, in agreement with recurrent admixture between wild and domestic animals. We also searched across the genome for loci under diversifying selection based on F-S(T) outlier tests. Candidate genes that may underlie differences in adaptation to specific environments and productive systems and phenotypic traits were detected in potentially selected genomic regions.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Genomic diversity, linkage disequilibrium and selection signatures in European local pig breeds assessed with a high density SNP chip",
volume = "9",
doi = "10.1038/s41598-019-49830-6"
}

Munoz, M., Bozzi, R., Garcia-Casco, J., Nunez, Y., Ribani, A., Franci, O., Garcia, F., Skrlep, M., Schiavo, G., Bovo, S., Utzeri, V. J., Charneca, R., Martins, J. M., Quintanilla, R., Tibau, J., Margeta, V., Djurkin-Kusec, I., Mercat, M. J., Riquet, J., Estelle, J., Zimmer, C., Razmaite, V., Araujo, J. P., Radović, Č., Savić, R., Karolyi, D., Gallo, M., Čandek-Potokar, M., Fernandez, A. I., Fontanesi, L.,& Ovilo, C.. (2019). Genomic diversity, linkage disequilibrium and selection signatures in European local pig breeds assessed with a high density SNP chip. in Scientific Reports
Nature Publishing Group, London., 9.
https://doi.org/10.1038/s41598-019-49830-6

Munoz M, Bozzi R, Garcia-Casco J, Nunez Y, Ribani A, Franci O, Garcia F, Skrlep M, Schiavo G, Bovo S, Utzeri VJ, Charneca R, Martins JM, Quintanilla R, Tibau J, Margeta V, Djurkin-Kusec I, Mercat MJ, Riquet J, Estelle J, Zimmer C, Razmaite V, Araujo JP, Radović Č, Savić R, Karolyi D, Gallo M, Čandek-Potokar M, Fernandez AI, Fontanesi L, Ovilo C. Genomic diversity, linkage disequilibrium and selection signatures in European local pig breeds assessed with a high density SNP chip. in Scientific Reports. 2019;9.
doi:10.1038/s41598-019-49830-6 .

Munoz, Maria, Bozzi, Riccardo, Garcia-Casco, Juan, Nunez, Yolanda, Ribani, A., Franci, O., Garcia, Fabian, Skrlep, Martin, Schiavo, Giuseppina, Bovo, Samuele, Utzeri, Valerio J., Charneca, R., Martins, Jose M., Quintanilla, Raquel, Tibau, J., Margeta, Vladimir, Djurkin-Kusec, Ivona, Mercat, Marie J., Riquet, Juliette, Estelle, Jordi, Zimmer, Christoph, Razmaite, Violeta, Araujo, Jose P., Radović, Čedomir, Savić, Radomir, Karolyi, Danijel, Gallo, Maurizio, Čandek-Potokar, Marjeta, Fernandez, Ana I, Fontanesi, Luca, Ovilo, Cristina, "Genomic diversity, linkage disequilibrium and selection signatures in European local pig breeds assessed with a high density SNP chip" in Scientific Reports, 9 (2019),
https://doi.org/10.1038/s41598-019-49830-6 . .

TY  - JOUR
AU  - Munoz, Maria
AU  - Bozzi, Riccardo
AU  - Garcia, Fabian
AU  - Nunez, Yolanda
AU  - Geraci, Claudia
AU  - Crovetti, Alessandro
AU  - Garcia-Casco, Juan
AU  - Alves, Estefania
AU  - Skrlep, Martin
AU  - Charneca, Rui
AU  - Martins, Jose M.
AU  - Quintanilla, Raquel
AU  - Tibau, Joan
AU  - Kusec, Goran
AU  - Djurkin-Kusec, Ivona
AU  - Mercat, Marie J.
AU  - Riquet, Juliette
AU  - Estelle, Jordi
AU  - Zimmer, Christoph
AU  - Razmaite, Violeta
AU  - Araujo, Jose P.
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Čandek-Potokar, Marjeta
AU  - Fontanesi, Luca
AU  - Fernandez, Ana I
AU  - Ovilo, Cristina
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4698
AB  - The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, ApuloCalabrese, Basque, Bisara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Kr6kopolje (Kr6kopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwabisch-Hallisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje). We successfully analyzed allelic variation in 39 polymorphisms, located in 33 candidate genes. Results provide relevant information regarding genetic diversity and segregation of SNPs associated to production and quality traits. Coat color and morphological trait-genes that show low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for association studies as well as breeding programs. For instance, we observed predominance of alleles that might be unfavorable for disease resistance and boar taint in most breeds and segregation of many alleles involved in meat quality, fatness and growth traits. Overall, these findings provide a detailed catalogue of segregating candidate SNPs in 20 European local pig breeds that may be useful for traceability purposes, for association studies and for breeding schemes. Population genetic analyses based on these candidate genes are able to uncover some clues regarding the hidden genetic substructure of these populations, as the extreme genetic closeness between Iberian and Alentejana breeds and an uneven admixture of the breeds studied. The results are in agreement with available knowledge regarding breed history and management, although largest panels of neutral markers should be employed to get a deeper understanding of the population's structure and relationships.
PB  - Public Library Science, San Francisco
T2  - Plos One
T1  - Diversity across major and candidate genes in European local pig breeds
IS  - 11
VL  - 13
DO  - 10.1371/journal.pone.0207475
ER  - 

@article{
author = "Munoz, Maria and Bozzi, Riccardo and Garcia, Fabian and Nunez, Yolanda and Geraci, Claudia and Crovetti, Alessandro and Garcia-Casco, Juan and Alves, Estefania and Skrlep, Martin and Charneca, Rui and Martins, Jose M. and Quintanilla, Raquel and Tibau, Joan and Kusec, Goran and Djurkin-Kusec, Ivona and Mercat, Marie J. and Riquet, Juliette and Estelle, Jordi and Zimmer, Christoph and Razmaite, Violeta and Araujo, Jose P. and Radović, Čedomir and Savić, Radomir and Karolyi, Danijel and Gallo, Maurizio and Čandek-Potokar, Marjeta and Fontanesi, Luca and Fernandez, Ana I and Ovilo, Cristina",
year = "2018",
abstract = "The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, ApuloCalabrese, Basque, Bisara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Kr6kopolje (Kr6kopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwabisch-Hallisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje). We successfully analyzed allelic variation in 39 polymorphisms, located in 33 candidate genes. Results provide relevant information regarding genetic diversity and segregation of SNPs associated to production and quality traits. Coat color and morphological trait-genes that show low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for association studies as well as breeding programs. For instance, we observed predominance of alleles that might be unfavorable for disease resistance and boar taint in most breeds and segregation of many alleles involved in meat quality, fatness and growth traits. Overall, these findings provide a detailed catalogue of segregating candidate SNPs in 20 European local pig breeds that may be useful for traceability purposes, for association studies and for breeding schemes. Population genetic analyses based on these candidate genes are able to uncover some clues regarding the hidden genetic substructure of these populations, as the extreme genetic closeness between Iberian and Alentejana breeds and an uneven admixture of the breeds studied. The results are in agreement with available knowledge regarding breed history and management, although largest panels of neutral markers should be employed to get a deeper understanding of the population's structure and relationships.",
publisher = "Public Library Science, San Francisco",
journal = "Plos One",
title = "Diversity across major and candidate genes in European local pig breeds",
number = "11",
volume = "13",
doi = "10.1371/journal.pone.0207475"
}

Munoz, M., Bozzi, R., Garcia, F., Nunez, Y., Geraci, C., Crovetti, A., Garcia-Casco, J., Alves, E., Skrlep, M., Charneca, R., Martins, J. M., Quintanilla, R., Tibau, J., Kusec, G., Djurkin-Kusec, I., Mercat, M. J., Riquet, J., Estelle, J., Zimmer, C., Razmaite, V., Araujo, J. P., Radović, Č., Savić, R., Karolyi, D., Gallo, M., Čandek-Potokar, M., Fontanesi, L., Fernandez, A. I.,& Ovilo, C.. (2018). Diversity across major and candidate genes in European local pig breeds. in Plos One
Public Library Science, San Francisco., 13(11).
https://doi.org/10.1371/journal.pone.0207475

Munoz M, Bozzi R, Garcia F, Nunez Y, Geraci C, Crovetti A, Garcia-Casco J, Alves E, Skrlep M, Charneca R, Martins JM, Quintanilla R, Tibau J, Kusec G, Djurkin-Kusec I, Mercat MJ, Riquet J, Estelle J, Zimmer C, Razmaite V, Araujo JP, Radović Č, Savić R, Karolyi D, Gallo M, Čandek-Potokar M, Fontanesi L, Fernandez AI, Ovilo C. Diversity across major and candidate genes in European local pig breeds. in Plos One. 2018;13(11).
doi:10.1371/journal.pone.0207475 .

Munoz, Maria, Bozzi, Riccardo, Garcia, Fabian, Nunez, Yolanda, Geraci, Claudia, Crovetti, Alessandro, Garcia-Casco, Juan, Alves, Estefania, Skrlep, Martin, Charneca, Rui, Martins, Jose M., Quintanilla, Raquel, Tibau, Joan, Kusec, Goran, Djurkin-Kusec, Ivona, Mercat, Marie J., Riquet, Juliette, Estelle, Jordi, Zimmer, Christoph, Razmaite, Violeta, Araujo, Jose P., Radović, Čedomir, Savić, Radomir, Karolyi, Danijel, Gallo, Maurizio, Čandek-Potokar, Marjeta, Fontanesi, Luca, Fernandez, Ana I, Ovilo, Cristina, "Diversity across major and candidate genes in European local pig breeds" in Plos One, 13, no. 11 (2018),
https://doi.org/10.1371/journal.pone.0207475 . .

TY  - JOUR
AU  - Radović, Čedomir
AU  - Petrović, M.
AU  - Savić, Radomir
AU  - Gogić, Marija
AU  - Lukić, M.
AU  - Stanišić, Nikola
AU  - Čandek-Potokar, Marjeta
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4509
AB  - The aim of this study was to examine the growth potential of indigenous pig breeds Mangalitsa (swallow bellied; n=12) and Moravka (n=10). The investigation included male castrates (surgical). Pigs were transferred from different farms to the institute’s stable where pigs were housed. The fattening period started at an average weight of 24.3 kg and 173.1 days of age for Mangalitsa pigs and 29.9 and 170.8 days for Moravka pigs, respectively and finished at average weight of 115.5 kg and 357 days of age for Mangalitsa pigs and 131.3 kg and 363 days for Moravka pigs, respectively. During observation study Moravka pigs had 13% higher (but not significantly) growth rate than Mangalitsa breed (545 vs. 480 g/day, P=0.22). The maximal growth rate observed for Mangalitsa was 690 g and for Moravka 607 g in the period corresponding to average body weight (end of period) of 79 and 89 kg, respectively. Initial (at start of the study) difference in growth rate between Mangalitsa and Moravka was propagated along the study duration resulting in significant difference in body weight at slaughter (P lt 0.05).
PB  - University of Zagreb
T2  - Agriculturae Conspectus Scientificus
T1  - Growth potential of Serbian local pig breeds Mangalitsa and Moravka
EP  - 220
IS  - 3 Special Issue 2
SP  - 217
VL  - 82
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4509
ER  - 

@article{
author = "Radović, Čedomir and Petrović, M. and Savić, Radomir and Gogić, Marija and Lukić, M. and Stanišić, Nikola and Čandek-Potokar, Marjeta",
year = "2017",
abstract = "The aim of this study was to examine the growth potential of indigenous pig breeds Mangalitsa (swallow bellied; n=12) and Moravka (n=10). The investigation included male castrates (surgical). Pigs were transferred from different farms to the institute’s stable where pigs were housed. The fattening period started at an average weight of 24.3 kg and 173.1 days of age for Mangalitsa pigs and 29.9 and 170.8 days for Moravka pigs, respectively and finished at average weight of 115.5 kg and 357 days of age for Mangalitsa pigs and 131.3 kg and 363 days for Moravka pigs, respectively. During observation study Moravka pigs had 13% higher (but not significantly) growth rate than Mangalitsa breed (545 vs. 480 g/day, P=0.22). The maximal growth rate observed for Mangalitsa was 690 g and for Moravka 607 g in the period corresponding to average body weight (end of period) of 79 and 89 kg, respectively. Initial (at start of the study) difference in growth rate between Mangalitsa and Moravka was propagated along the study duration resulting in significant difference in body weight at slaughter (P lt 0.05).",
publisher = "University of Zagreb",
journal = "Agriculturae Conspectus Scientificus",
title = "Growth potential of Serbian local pig breeds Mangalitsa and Moravka",
pages = "220-217",
number = "3 Special Issue 2",
volume = "82",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4509"
}

Radović, Č., Petrović, M., Savić, R., Gogić, M., Lukić, M., Stanišić, N.,& Čandek-Potokar, M.. (2017). Growth potential of Serbian local pig breeds Mangalitsa and Moravka. in Agriculturae Conspectus Scientificus
University of Zagreb., 82(3 Special Issue 2), 217-220.
https://hdl.handle.net/21.15107/rcub_agrospace_4509

Radović Č, Petrović M, Savić R, Gogić M, Lukić M, Stanišić N, Čandek-Potokar M. Growth potential of Serbian local pig breeds Mangalitsa and Moravka. in Agriculturae Conspectus Scientificus. 2017;82(3 Special Issue 2):217-220.
https://hdl.handle.net/21.15107/rcub_agrospace_4509 .

Radović, Čedomir, Petrović, M., Savić, Radomir, Gogić, Marija, Lukić, M., Stanišić, Nikola, Čandek-Potokar, Marjeta, "Growth potential of Serbian local pig breeds Mangalitsa and Moravka" in Agriculturae Conspectus Scientificus, 82, no. 3 Special Issue 2 (2017):217-220,
https://hdl.handle.net/21.15107/rcub_agrospace_4509 .

TY  - JOUR
AU  - Čandek-Potokar, Marjeta
AU  - Nieto, R.
AU  - Pugliese, C.
AU  - Araujo, Jose P.
AU  - Charneca, R.
AU  - Garcia-Casco, Juan
AU  - González Sánchez, E.
AU  - Hernandez-Garcia, F.I.
AU  - Izquierdo, M.
AU  - Karolyi, Danijel
AU  - Kusec, Goran
AU  - Lebret, B.
AU  - Mercat, Marie J.
AU  - Petig, M.
AU  - Radović, Čedomir
AU  - Savić, R.
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4512
AB  - TREASURE is a research and innovation project of European Union’s Horizon 2020 programme which is devoted to traditional genetic resources in pig production with aim to improve their potentials for enhanced use. Studying and improving management of local pig breeds in their production systems is one of the challenges in which we address their performances and nutrition with special attention on locally available feeding resources and innovative practices aiming to improved welfare. For that purpose 15 experiments on 12 breeds were designed in the project, which are hereafter shortly presented. Their concepts and main objectives with some highlights on already available results are described.
PB  - University of Zagreb
T2  - Agriculturae Conspectus Scientificus
T1  - Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE
EP  - 131
IS  - 2 Special Issue 1
SP  - 127
VL  - 82
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4512
ER  - 

@article{
author = "Čandek-Potokar, Marjeta and Nieto, R. and Pugliese, C. and Araujo, Jose P. and Charneca, R. and Garcia-Casco, Juan and González Sánchez, E. and Hernandez-Garcia, F.I. and Izquierdo, M. and Karolyi, Danijel and Kusec, Goran and Lebret, B. and Mercat, Marie J. and Petig, M. and Radović, Čedomir and Savić, R.",
year = "2017",
abstract = "TREASURE is a research and innovation project of European Union’s Horizon 2020 programme which is devoted to traditional genetic resources in pig production with aim to improve their potentials for enhanced use. Studying and improving management of local pig breeds in their production systems is one of the challenges in which we address their performances and nutrition with special attention on locally available feeding resources and innovative practices aiming to improved welfare. For that purpose 15 experiments on 12 breeds were designed in the project, which are hereafter shortly presented. Their concepts and main objectives with some highlights on already available results are described.",
publisher = "University of Zagreb",
journal = "Agriculturae Conspectus Scientificus",
title = "Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE",
pages = "131-127",
number = "2 Special Issue 1",
volume = "82",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4512"
}

Čandek-Potokar, M., Nieto, R., Pugliese, C., Araujo, J. P., Charneca, R., Garcia-Casco, J., González Sánchez, E., Hernandez-Garcia, F.I., Izquierdo, M., Karolyi, D., Kusec, G., Lebret, B., Mercat, M. J., Petig, M., Radović, Č.,& Savić, R.. (2017). Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE. in Agriculturae Conspectus Scientificus
University of Zagreb., 82(2 Special Issue 1), 127-131.
https://hdl.handle.net/21.15107/rcub_agrospace_4512

Čandek-Potokar M, Nieto R, Pugliese C, Araujo JP, Charneca R, Garcia-Casco J, González Sánchez E, Hernandez-Garcia F, Izquierdo M, Karolyi D, Kusec G, Lebret B, Mercat MJ, Petig M, Radović Č, Savić R. Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE. in Agriculturae Conspectus Scientificus. 2017;82(2 Special Issue 1):127-131.
https://hdl.handle.net/21.15107/rcub_agrospace_4512 .

Čandek-Potokar, Marjeta, Nieto, R., Pugliese, C., Araujo, Jose P., Charneca, R., Garcia-Casco, Juan, González Sánchez, E., Hernandez-Garcia, F.I., Izquierdo, M., Karolyi, Danijel, Kusec, Goran, Lebret, B., Mercat, Marie J., Petig, M., Radović, Čedomir, Savić, R., "Local pig breeds: Nutritional requirements, innovative practices and local feeding resources as challenges in project TREASURE" in Agriculturae Conspectus Scientificus, 82, no. 2 Special Issue 1 (2017):127-131,
https://hdl.handle.net/21.15107/rcub_agrospace_4512 .