TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6013
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
SP  - 803789
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Danijela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
pages = "803789",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gašić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, D.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media S.A.., 12, 803789.
https://doi.org/10.3389/fmicb.2021.803789

Gašić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić D, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12:803789.
doi:10.3389/fmicb.2021.803789 .

Gašić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Danijela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12 (2022):803789,
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Ristić, Danijela
AU  - Vucurović, Ivan
AU  - Krstić, Branka
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5338
AB  - A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia
EP  - 797
IS  - 3
SP  - 787
VL  - 102
DO  - 10.1007/s42161-020-00504-7
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Ristić, Danijela and Vucurović, Ivan and Krstić, Branka",
year = "2020",
abstract = "A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia",
pages = "797-787",
number = "3",
volume = "102",
doi = "10.1007/s42161-020-00504-7"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Ristić, D., Vucurović, I.,& Krstić, B.. (2020). Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology
Springer, New York., 102(3), 787-797.
https://doi.org/10.1007/s42161-020-00504-7

Stanković I, Vučurović A, Zečević K, Petrović B, Ristić D, Vucurović I, Krstić B. Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology. 2020;102(3):787-797.
doi:10.1007/s42161-020-00504-7 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Ristić, Danijela, Vucurović, Ivan, Krstić, Branka, "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia" in Journal of Plant Pathology, 102, no. 3 (2020):787-797,
https://doi.org/10.1007/s42161-020-00504-7 . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vucurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Pfaf-Dolovac, Erika
AU  - Aleksić, Goran
AU  - Vučurović, Ana
AU  - Starović, Mira
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5042
AB  - It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.
PB  - Springer, Dordrecht
T2  - Potato Research
T1  - The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops
EP  - 46
IS  - 1
SP  - 31
VL  - 62
DO  - 10.1007/s11540-018-9395-y
ER  - 

@article{
author = "Ristić, Danijela and Vucurović, Ivan and Kuzmanović, Slobodan and Pfaf-Dolovac, Erika and Aleksić, Goran and Vučurović, Ana and Starović, Mira",
year = "2019",
abstract = "It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.",
publisher = "Springer, Dordrecht",
journal = "Potato Research",
title = "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops",
pages = "46-31",
number = "1",
volume = "62",
doi = "10.1007/s11540-018-9395-y"
}

Ristić, D., Vucurović, I., Kuzmanović, S., Pfaf-Dolovac, E., Aleksić, G., Vučurović, A.,& Starović, M.. (2019). The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research
Springer, Dordrecht., 62(1), 31-46.
https://doi.org/10.1007/s11540-018-9395-y

Ristić D, Vucurović I, Kuzmanović S, Pfaf-Dolovac E, Aleksić G, Vučurović A, Starović M. The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research. 2019;62(1):31-46.
doi:10.1007/s11540-018-9395-y .

Ristić, Danijela, Vucurović, Ivan, Kuzmanović, Slobodan, Pfaf-Dolovac, Erika, Aleksić, Goran, Vučurović, Ana, Starović, Mira, "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops" in Potato Research, 62, no. 1 (2019):31-46,
https://doi.org/10.1007/s11540-018-9395-y . .

TY  - JOUR
AU  - Stevanović, Milos
AU  - Ristić, Danijela
AU  - Zivković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5044
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 

@article{
author = "Stevanović, Milos and Ristić, Danijela and Zivković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}

Stevanović, M., Ristić, D., Zivković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE

Stevanović M, Ristić D, Zivković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .

Stevanović, Milos, Ristić, Danijela, Zivković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Krstić, Branka
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4826
AB  - The number of virus species infecting grapevine has increased continuously and more than 60 species are described to infect grapevine. The global expansion of the grape and wine industry has seen a parallel increase in the incidence and economic impact of grapevine leafroll disease (GLD). GLD can cause reduced plant vigor and longevity, and significant losses in both fruit yield and quality. Our knowledge of the nature of the disease is still quite limited due to a variety of challenges related to the complexity of this virus disease, the association of several genetically and serologically distinct GLRaVs, and contrasting symptoms in red- and white-berried cultivars. Within grapevine leafroll complex (Grapevine leafroll-associated viruses, GLRaVs) five different species are classified in the family Closteroviridae: one is classified in the genus Closterovirus (GLRaV-2), three in the genus Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, and his strains: GLRaV-4 strain 5, GLRaV-4 strain 6, GLRaV-4 strain 9, GLRaV-4 strain Pr, GLRaV-4 strain De and GLRaV-4 strain Car) and one in the genus Velarivirus (GLRaV-7). GLRaVs are transmitted by grafting and propagation material (rootstock and scion) and some species by mealybugs. Grapevine leafroll-associated viruses usually occur in a mixed infection, and are often present in a mixed infection with other grape viruses. Early research carried out in vineyards in Serbia determined the presence of GLRV-1, -2 and -3, with GLRaV-3 being the most prevalent. If spread of the viruses transsmited by vectors within plantings exists, roguing or removal of infected vines, mealybug control, and sanitation measures can reduce new infections.
AB  - Brojnost virusa infektivnih za vinovu lozu stalno se povećava, tako da je do sada opisano više od 60 vrsta koji mogu da zaraze vinovu lozu. Paralelno sa globalnom ekspanzijom vinogradarske i vinarske industrije zapaženo je povećanje učestalosti i ekonomskog značaja oboljenja uvijenosti lišća vinove loze (Grapevine Leafroll Disease, GLD). GLD, osim što smanjuje vitalnost i dugovečnost vinove loze, značajno smanjuje prinos i kvalitet grožđa. Epidemiologija oboljenja je nedovoljno istražena zbog kompleksnosti ove bolesti, genetički i serološki različitih vrsta unutar kompleksa i različitosti simptoma kod crvenih i belih sorti vinove loze. Кompleks virusa prouzrokovača uvijenosti lišća vinove loze (Grapevine leafrollassociated viruses, GLRaVs) čini pet različitih virusa koji pripadaju familiji Closteroviridae: jedna vrsta koja pripada rodu Closterovirus (GLRaV-2), tri rodu Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, i njegovi sojevi: GLRaV-4 soj 5, GLRaV-4 soj 6, GLRaV-4 soj 9, GLRaV-4 soj Pr, GLRaV-4 soj De i GLRaV-4 soj Car) i jedna vrsta iz roda Velarivirus (GLRaV-7). GLRaVs se prenose kalemljenjem i propagativnim materijalom (podloga i vijoka), a neke vrste i štitastim vašima. Virusi koji izazivaju uvijenost lišća se obično javljaju u mešanoj infekciji, a često su prisutni i u mešanoj infekciji sa drugim virusima vinove loze. Ranija istraživanja sprovedena u vinogorjima u Srbiji utvrdila su prisustvo GLRV-1, -2 i -3, pri čemu je GLRaV-3 bio najzastupljeniji. Ukoliko je širenje virusa unutar zasada putem vektora izraženo, krčenje ili uklanjanje zaraženih čokota, kontrola štitastih vaši i adekvatne sanitarne mere mogu redukovati nove infekcije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Grapevine Leafroll complex
T1  - Kompleks virusa prouzrokovača uvijenosti lišća vinove loze
EP  - 690
IS  - 6
SP  - 681
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4826
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Krstić, Branka",
year = "2018",
abstract = "The number of virus species infecting grapevine has increased continuously and more than 60 species are described to infect grapevine. The global expansion of the grape and wine industry has seen a parallel increase in the incidence and economic impact of grapevine leafroll disease (GLD). GLD can cause reduced plant vigor and longevity, and significant losses in both fruit yield and quality. Our knowledge of the nature of the disease is still quite limited due to a variety of challenges related to the complexity of this virus disease, the association of several genetically and serologically distinct GLRaVs, and contrasting symptoms in red- and white-berried cultivars. Within grapevine leafroll complex (Grapevine leafroll-associated viruses, GLRaVs) five different species are classified in the family Closteroviridae: one is classified in the genus Closterovirus (GLRaV-2), three in the genus Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, and his strains: GLRaV-4 strain 5, GLRaV-4 strain 6, GLRaV-4 strain 9, GLRaV-4 strain Pr, GLRaV-4 strain De and GLRaV-4 strain Car) and one in the genus Velarivirus (GLRaV-7). GLRaVs are transmitted by grafting and propagation material (rootstock and scion) and some species by mealybugs. Grapevine leafroll-associated viruses usually occur in a mixed infection, and are often present in a mixed infection with other grape viruses. Early research carried out in vineyards in Serbia determined the presence of GLRV-1, -2 and -3, with GLRaV-3 being the most prevalent. If spread of the viruses transsmited by vectors within plantings exists, roguing or removal of infected vines, mealybug control, and sanitation measures can reduce new infections., Brojnost virusa infektivnih za vinovu lozu stalno se povećava, tako da je do sada opisano više od 60 vrsta koji mogu da zaraze vinovu lozu. Paralelno sa globalnom ekspanzijom vinogradarske i vinarske industrije zapaženo je povećanje učestalosti i ekonomskog značaja oboljenja uvijenosti lišća vinove loze (Grapevine Leafroll Disease, GLD). GLD, osim što smanjuje vitalnost i dugovečnost vinove loze, značajno smanjuje prinos i kvalitet grožđa. Epidemiologija oboljenja je nedovoljno istražena zbog kompleksnosti ove bolesti, genetički i serološki različitih vrsta unutar kompleksa i različitosti simptoma kod crvenih i belih sorti vinove loze. Кompleks virusa prouzrokovača uvijenosti lišća vinove loze (Grapevine leafrollassociated viruses, GLRaVs) čini pet različitih virusa koji pripadaju familiji Closteroviridae: jedna vrsta koja pripada rodu Closterovirus (GLRaV-2), tri rodu Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, i njegovi sojevi: GLRaV-4 soj 5, GLRaV-4 soj 6, GLRaV-4 soj 9, GLRaV-4 soj Pr, GLRaV-4 soj De i GLRaV-4 soj Car) i jedna vrsta iz roda Velarivirus (GLRaV-7). GLRaVs se prenose kalemljenjem i propagativnim materijalom (podloga i vijoka), a neke vrste i štitastim vašima. Virusi koji izazivaju uvijenost lišća se obično javljaju u mešanoj infekciji, a često su prisutni i u mešanoj infekciji sa drugim virusima vinove loze. Ranija istraživanja sprovedena u vinogorjima u Srbiji utvrdila su prisustvo GLRV-1, -2 i -3, pri čemu je GLRaV-3 bio najzastupljeniji. Ukoliko je širenje virusa unutar zasada putem vektora izraženo, krčenje ili uklanjanje zaraženih čokota, kontrola štitastih vaši i adekvatne sanitarne mere mogu redukovati nove infekcije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Grapevine Leafroll complex, Kompleks virusa prouzrokovača uvijenosti lišća vinove loze",
pages = "690-681",
number = "6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4826"
}

Ristić, D., Vučurović, I., Stanković, I., Vučurović, A., Zečević, K.,& Krstić, B.. (2018). Grapevine Leafroll complex. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 46(6), 681-690.
https://hdl.handle.net/21.15107/rcub_agrospace_4826

Ristić D, Vučurović I, Stanković I, Vučurović A, Zečević K, Krstić B. Grapevine Leafroll complex. in Biljni lekar. 2018;46(6):681-690.
https://hdl.handle.net/21.15107/rcub_agrospace_4826 .

Ristić, Danijela, Vučurović, Ivan, Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Krstić, Branka, "Grapevine Leafroll complex" in Biljni lekar, 46, no. 6 (2018):681-690,
https://hdl.handle.net/21.15107/rcub_agrospace_4826 .

TY  - JOUR
AU  - Vučurović, Ivan
AU  - Nikolić, Dušan
AU  - Radović, Nikola
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4603
AB  - Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.
AB  - Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Incidence and distribution of leek yellow stripe virus in allium crops in Serbia
T1  - Prisustvo i rasprostranjenost virusa žute prugavosti praziluka u usevima različitih vrsta lukova u Srbiji
EP  - 155
IS  - 3-4
SP  - 145
VL  - 32
DO  - 10.2298/PIF1704145V
ER  - 

@article{
author = "Vučurović, Ivan and Nikolić, Dušan and Radović, Nikola and Vučurović, Ana and Ristić, Danijela and Krstić, Branka and Stanković, Ivana",
year = "2017",
abstract = "Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E., Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia, Prisustvo i rasprostranjenost virusa žute prugavosti praziluka u usevima različitih vrsta lukova u Srbiji",
pages = "155-145",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704145V"
}

Vučurović, I., Nikolić, D., Radović, N., Vučurović, A., Ristić, D., Krstić, B.,& Stanković, I.. (2017). Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 32(3-4), 145-155.
https://doi.org/10.2298/PIF1704145V

Vučurović I, Nikolić D, Radović N, Vučurović A, Ristić D, Krstić B, Stanković I. Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina. 2017;32(3-4):145-155.
doi:10.2298/PIF1704145V .

Vučurović, Ivan, Nikolić, Dušan, Radović, Nikola, Vučurović, Ana, Ristić, Danijela, Krstić, Branka, Stanković, Ivana, "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):145-155,
https://doi.org/10.2298/PIF1704145V . .

TY  - JOUR
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Nikolić, D.
AU  - Ristić, Danijela
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3431
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cucumber mosaic virus in Tulipa sp in Serbia
EP  - 1449
IS  - 10
SP  - 1449
VL  - 98
DO  - 10.1094/PDIS-05-14-0527-PDN
ER  - 

@article{
author = "Milojević, Katarina and Stanković, Ivana and Vučurović, Ana and Nikolić, D. and Ristić, Danijela and Bulajić, Aleksandra and Krstić, Branka",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cucumber mosaic virus in Tulipa sp in Serbia",
pages = "1449-1449",
number = "10",
volume = "98",
doi = "10.1094/PDIS-05-14-0527-PDN"
}

Milojević, K., Stanković, I., Vučurović, A., Nikolić, D., Ristić, D., Bulajić, A.,& Krstić, B.. (2014). First Report of Cucumber mosaic virus in Tulipa sp in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(10), 1449-1449.
https://doi.org/10.1094/PDIS-05-14-0527-PDN

Milojević K, Stanković I, Vučurović A, Nikolić D, Ristić D, Bulajić A, Krstić B. First Report of Cucumber mosaic virus in Tulipa sp in Serbia. in Plant Disease. 2014;98(10):1449-1449.
doi:10.1094/PDIS-05-14-0527-PDN .

Milojević, Katarina, Stanković, Ivana, Vučurović, Ana, Nikolić, D., Ristić, Danijela, Bulajić, Aleksandra, Krstić, Branka, "First Report of Cucumber mosaic virus in Tulipa sp in Serbia" in Plant Disease, 98, no. 10 (2014):1449-1449,
https://doi.org/10.1094/PDIS-05-14-0527-PDN . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Vučurović, Ana
AU  - Milojević, Katarina
AU  - Nikolić, D.
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3404
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium Wilt of Strawberry Caused by Fusarium oxysporum in Serbia
EP  - 1435
IS  - 10
SP  - 1435
VL  - 98
DO  - 10.1094/PDIS-04-14-0346-PDN
ER  - 

@article{
author = "Stanković, Ivana and Ristić, Danijela and Vučurović, Ana and Milojević, Katarina and Nikolić, D. and Krstić, Branka and Bulajić, Aleksandra",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium Wilt of Strawberry Caused by Fusarium oxysporum in Serbia",
pages = "1435-1435",
number = "10",
volume = "98",
doi = "10.1094/PDIS-04-14-0346-PDN"
}

Stanković, I., Ristić, D., Vučurović, A., Milojević, K., Nikolić, D., Krstić, B.,& Bulajić, A.. (2014). First Report of Fusarium Wilt of Strawberry Caused by Fusarium oxysporum in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(10), 1435-1435.
https://doi.org/10.1094/PDIS-04-14-0346-PDN

Stanković I, Ristić D, Vučurović A, Milojević K, Nikolić D, Krstić B, Bulajić A. First Report of Fusarium Wilt of Strawberry Caused by Fusarium oxysporum in Serbia. in Plant Disease. 2014;98(10):1435-1435.
doi:10.1094/PDIS-04-14-0346-PDN .

Stanković, Ivana, Ristić, Danijela, Vučurović, Ana, Milojević, Katarina, Nikolić, D., Krstić, Branka, Bulajić, Aleksandra, "First Report of Fusarium Wilt of Strawberry Caused by Fusarium oxysporum in Serbia" in Plant Disease, 98, no. 10 (2014):1435-1435,
https://doi.org/10.1094/PDIS-04-14-0346-PDN . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Ivanović, Mirko S.
AU  - Krstić, Branka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3536
AB  - Tomato spotted wilt virus is an economically important pathogen in several potato producing areas in the world. Although natural infections of potato in Serbia have not been detected, two TSWV isolates from tobacco, with different biological features were compared based on NSm gene sequence analysis and they were used for mechanical and graft inoculations to evaluate the susceptibility of five popular potato cultivars: 'Riviera', 'Arnova', 'Curoda', 'Kondor' and 'Aladin'. Both TSWV isolates infected all tested potato cultivars. Statistical analyses showed that the rate of TSWV transmission from infected foliage to tubers and from infected tubers to progeny plants was affected by cultivars, while virus transmission efficiency was independent of investigated isolates. Different potato tissues used for sample preparation did not influence virus detection by ELISA, but composite sampling from six different tuber parts is recommended since it was the most reliable.
PB  - Springer, New York
T2  - American Journal of Potato Research
T1  - Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission
EP  - 194
IS  - 2
SP  - 186
VL  - 91
DO  - 10.1007/s12230-013-9337-9
ER  - 

@article{
author = "Bulajić, Aleksandra and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Ivanović, Mirko S. and Krstić, Branka",
year = "2014",
abstract = "Tomato spotted wilt virus is an economically important pathogen in several potato producing areas in the world. Although natural infections of potato in Serbia have not been detected, two TSWV isolates from tobacco, with different biological features were compared based on NSm gene sequence analysis and they were used for mechanical and graft inoculations to evaluate the susceptibility of five popular potato cultivars: 'Riviera', 'Arnova', 'Curoda', 'Kondor' and 'Aladin'. Both TSWV isolates infected all tested potato cultivars. Statistical analyses showed that the rate of TSWV transmission from infected foliage to tubers and from infected tubers to progeny plants was affected by cultivars, while virus transmission efficiency was independent of investigated isolates. Different potato tissues used for sample preparation did not influence virus detection by ELISA, but composite sampling from six different tuber parts is recommended since it was the most reliable.",
publisher = "Springer, New York",
journal = "American Journal of Potato Research",
title = "Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission",
pages = "194-186",
number = "2",
volume = "91",
doi = "10.1007/s12230-013-9337-9"
}

Bulajić, A., Stanković, I., Vučurović, A., Ristić, D., Milojević, K., Ivanović, M. S.,& Krstić, B.. (2014). Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission. in American Journal of Potato Research
Springer, New York., 91(2), 186-194.
https://doi.org/10.1007/s12230-013-9337-9

Bulajić A, Stanković I, Vučurović A, Ristić D, Milojević K, Ivanović MS, Krstić B. Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission. in American Journal of Potato Research. 2014;91(2):186-194.
doi:10.1007/s12230-013-9337-9 .

Bulajić, Aleksandra, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Ivanović, Mirko S., Krstić, Branka, "Tomato Spotted Wilt Virus - Potato Cultivar Susceptibility and Tuber Transmission" in American Journal of Potato Research, 91, no. 2 (2014):186-194,
https://doi.org/10.1007/s12230-013-9337-9 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3556
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 

@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}

Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G

Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .

Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .

TY  - JOUR
AU  - Nikolić, D.
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3312
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Tomato spotted wilt virus on Brugmansia sp in Serbia.
EP  - 850
IS  - 6
SP  - 850
VL  - 97
DO  - 10.1094/PDIS-10-12-0937-PDN
ER  - 

@article{
author = "Nikolić, D. and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Bulajić, Aleksandra and Krstić, Branka",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Tomato spotted wilt virus on Brugmansia sp in Serbia.",
pages = "850-850",
number = "6",
volume = "97",
doi = "10.1094/PDIS-10-12-0937-PDN"
}

Nikolić, D., Stanković, I., Vučurović, A., Ristić, D., Milojević, K., Bulajić, A.,& Krstić, B.. (2013). First Report of Tomato spotted wilt virus on Brugmansia sp in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(6), 850-850.
https://doi.org/10.1094/PDIS-10-12-0937-PDN

Nikolić D, Stanković I, Vučurović A, Ristić D, Milojević K, Bulajić A, Krstić B. First Report of Tomato spotted wilt virus on Brugmansia sp in Serbia.. in Plant Disease. 2013;97(6):850-850.
doi:10.1094/PDIS-10-12-0937-PDN .

Nikolić, D., Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Bulajić, Aleksandra, Krstić, Branka, "First Report of Tomato spotted wilt virus on Brugmansia sp in Serbia." in Plant Disease, 97, no. 6 (2013):850-850,
https://doi.org/10.1094/PDIS-10-12-0937-PDN . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Nikolić, D.
AU  - Krstić, Branka
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3303
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Tomato spotted wilt virus on Chrysanthemum in Serbia
EP  - 151
IS  - 1
SP  - 150
VL  - 97
DO  - 10.1094/PDIS-08-12-0778-PDN
ER  - 

@article{
author = "Stanković, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Nikolić, D. and Krstić, Branka",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Tomato spotted wilt virus on Chrysanthemum in Serbia",
pages = "151-150",
number = "1",
volume = "97",
doi = "10.1094/PDIS-08-12-0778-PDN"
}

Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Nikolić, D.,& Krstić, B.. (2013). First Report of Tomato spotted wilt virus on Chrysanthemum in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(1), 150-151.
https://doi.org/10.1094/PDIS-08-12-0778-PDN

Stanković I, Bulajić A, Vučurović A, Ristić D, Milojević K, Nikolić D, Krstić B. First Report of Tomato spotted wilt virus on Chrysanthemum in Serbia. in Plant Disease. 2013;97(1):150-151.
doi:10.1094/PDIS-08-12-0778-PDN .

Stanković, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Nikolić, D., Krstić, Branka, "First Report of Tomato spotted wilt virus on Chrysanthemum in Serbia" in Plant Disease, 97, no. 1 (2013):150-151,
https://doi.org/10.1094/PDIS-08-12-0778-PDN . .

TY  - JOUR
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milošević, D.
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3172
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia.
EP  - 1004
IS  - 7
SP  - 1004
VL  - 97
DO  - 10.1094/PDIS-01-13-0089-PDN
ER  - 

@article{
author = "Milojević, Katarina and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Milošević, D. and Bulajić, Aleksandra and Krstić, Branka",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia.",
pages = "1004-1004",
number = "7",
volume = "97",
doi = "10.1094/PDIS-01-13-0089-PDN"
}

Milojević, K., Stanković, I., Vučurović, A., Ristić, D., Milošević, D., Bulajić, A.,& Krstić, B.. (2013). First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(7), 1004-1004.
https://doi.org/10.1094/PDIS-01-13-0089-PDN

Milojević K, Stanković I, Vučurović A, Ristić D, Milošević D, Bulajić A, Krstić B. First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia.. in Plant Disease. 2013;97(7):1004-1004.
doi:10.1094/PDIS-01-13-0089-PDN .

Milojević, Katarina, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Milošević, D., Bulajić, Aleksandra, Krstić, Branka, "First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia." in Plant Disease, 97, no. 7 (2013):1004-1004,
https://doi.org/10.1094/PDIS-01-13-0089-PDN . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Nikolić, D.
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3018
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Tomato spotted wilt virus Infecting Onion and Garlic in Serbia
EP  - 918
IS  - 6
SP  - 918
VL  - 96
DO  - 10.1094/PDIS-02-12-0157-PDN
ER  - 

@article{
author = "Stanković, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Nikolić, D. and Krstić, Branka",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Tomato spotted wilt virus Infecting Onion and Garlic in Serbia",
pages = "918-918",
number = "6",
volume = "96",
doi = "10.1094/PDIS-02-12-0157-PDN"
}

Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Nikolić, D.,& Krstić, B.. (2012). First Report of Tomato spotted wilt virus Infecting Onion and Garlic in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(6), 918-918.
https://doi.org/10.1094/PDIS-02-12-0157-PDN

Stanković I, Bulajić A, Vučurović A, Ristić D, Milojević K, Nikolić D, Krstić B. First Report of Tomato spotted wilt virus Infecting Onion and Garlic in Serbia. in Plant Disease. 2012;96(6):918-918.
doi:10.1094/PDIS-02-12-0157-PDN .

Stanković, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Nikolić, D., Krstić, Branka, "First Report of Tomato spotted wilt virus Infecting Onion and Garlic in Serbia" in Plant Disease, 96, no. 6 (2012):918-918,
https://doi.org/10.1094/PDIS-02-12-0157-PDN . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Nikolić, D.
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3000
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Zucchini yellow mosaic virus in Watermelon in Serbia
EP  - 149
IS  - 1
SP  - 149
VL  - 96
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3000
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Nikolić, D. and Berenji, Janoš and Krstić, Branka",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Zucchini yellow mosaic virus in Watermelon in Serbia",
pages = "149-149",
number = "1",
volume = "96",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3000"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Nikolić, D., Berenji, J.,& Krstić, B.. (2012). First Report of Zucchini yellow mosaic virus in Watermelon in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(1), 149-149.
https://hdl.handle.net/21.15107/rcub_agrospace_3000

Vučurović A, Bulajić A, Stanković I, Ristić D, Nikolić D, Berenji J, Krstić B. First Report of Zucchini yellow mosaic virus in Watermelon in Serbia. in Plant Disease. 2012;96(1):149-149.
https://hdl.handle.net/21.15107/rcub_agrospace_3000 .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Nikolić, D., Berenji, Janoš, Krstić, Branka, "First Report of Zucchini yellow mosaic virus in Watermelon in Serbia" in Plant Disease, 96, no. 1 (2012):149-149,
https://hdl.handle.net/21.15107/rcub_agrospace_3000 .

TY  - JOUR
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Nikolić, D.
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2990
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cucumber mosaic virus Infecting Watermelon in Serbia.
EP  - 1706
IS  - 11
SP  - 1706
VL  - 96
DO  - 10.1094/PDIS-07-12-0631-PDN
ER  - 

@article{
author = "Milojević, Katarina and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Nikolić, D. and Bulajić, Aleksandra and Krstić, Branka",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cucumber mosaic virus Infecting Watermelon in Serbia.",
pages = "1706-1706",
number = "11",
volume = "96",
doi = "10.1094/PDIS-07-12-0631-PDN"
}

Milojević, K., Stanković, I., Vučurović, A., Ristić, D., Nikolić, D., Bulajić, A.,& Krstić, B.. (2012). First Report of Cucumber mosaic virus Infecting Watermelon in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(11), 1706-1706.
https://doi.org/10.1094/PDIS-07-12-0631-PDN

Milojević K, Stanković I, Vučurović A, Ristić D, Nikolić D, Bulajić A, Krstić B. First Report of Cucumber mosaic virus Infecting Watermelon in Serbia.. in Plant Disease. 2012;96(11):1706-1706.
doi:10.1094/PDIS-07-12-0631-PDN .

Milojević, Katarina, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Nikolić, D., Bulajić, Aleksandra, Krstić, Branka, "First Report of Cucumber mosaic virus Infecting Watermelon in Serbia." in Plant Disease, 96, no. 11 (2012):1706-1706,
https://doi.org/10.1094/PDIS-07-12-0631-PDN . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2961
AB  - Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates
EP  - 947
IS  - 4
SP  - 935
VL  - 133
DO  - 10.1007/s10658-012-9964-x
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Jović, Jelena and Krstić, Branka",
year = "2012",
abstract = "Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates",
pages = "947-935",
number = "4",
volume = "133",
doi = "10.1007/s10658-012-9964-x"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J.,& Krstić, B.. (2012). Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology
Springer, Dordrecht., 133(4), 935-947.
https://doi.org/10.1007/s10658-012-9964-x

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Jović J, Krstić B. Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology. 2012;133(4):935-947.
doi:10.1007/s10658-012-9964-x .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Jović, Jelena, Krstić, Branka, "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates" in European Journal of Plant Pathology, 133, no. 4 (2012):935-947,
https://doi.org/10.1007/s10658-012-9964-x . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Bulajić, Aleksandra
AU  - Ristić, Danijela
AU  - Krstić, Branka
AU  - Jankova, Ratko M.
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2975
AB  - BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit
EP  - 3078
IS  - 15
SP  - 3072
VL  - 92
DO  - 10.1002/jsfa.5728
ER  - 

@article{
author = "Popović, Milica M. and Bulajić, Aleksandra and Ristić, Danijela and Krstić, Branka and Jankova, Ratko M. and Gavrović-Jankulović, Marija",
year = "2012",
abstract = "BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit",
pages = "3078-3072",
number = "15",
volume = "92",
doi = "10.1002/jsfa.5728"
}

Popović, M. M., Bulajić, A., Ristić, D., Krstić, B., Jankova, R. M.,& Gavrović-Jankulović, M.. (2012). In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture
Wiley-Blackwell, Hoboken., 92(15), 3072-3078.
https://doi.org/10.1002/jsfa.5728

Popović MM, Bulajić A, Ristić D, Krstić B, Jankova RM, Gavrović-Jankulović M. In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture. 2012;92(15):3072-3078.
doi:10.1002/jsfa.5728 .

Popović, Milica M., Bulajić, Aleksandra, Ristić, Danijela, Krstić, Branka, Jankova, Ratko M., Gavrović-Jankulović, Marija, "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit" in Journal of the Science of Food and Agriculture, 92, no. 15 (2012):3072-3078,
https://doi.org/10.1002/jsfa.5728 . .

TY  - JOUR
AU  - Bulajić, A.
AU  - Vučurović, A.
AU  - Stanković, I.
AU  - Ristić, D.
AU  - Jović, J.
AU  - Stojković, B.
AU  - Krstić, B.
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2724
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Plasmopara obducens on Impatiens walleriana in Serbia
EP  - 491
IS  - 4
SP  - 491
VL  - 95
DO  - 10.1094/PDIS-10-10-0731
ER  - 

@article{
author = "Bulajić, A. and Vučurović, A. and Stanković, I. and Ristić, D. and Jović, J. and Stojković, B. and Krstić, B.",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Plasmopara obducens on Impatiens walleriana in Serbia",
pages = "491-491",
number = "4",
volume = "95",
doi = "10.1094/PDIS-10-10-0731"
}

Bulajić, A., Vučurović, A., Stanković, I., Ristić, D., Jović, J., Stojković, B.,& Krstić, B.. (2011). First Report of Plasmopara obducens on Impatiens walleriana in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(4), 491-491.
https://doi.org/10.1094/PDIS-10-10-0731

Bulajić A, Vučurović A, Stanković I, Ristić D, Jović J, Stojković B, Krstić B. First Report of Plasmopara obducens on Impatiens walleriana in Serbia. in Plant Disease. 2011;95(4):491-491.
doi:10.1094/PDIS-10-10-0731 .

Bulajić, A., Vučurović, A., Stanković, I., Ristić, D., Jović, J., Stojković, B., Krstić, B., "First Report of Plasmopara obducens on Impatiens walleriana in Serbia" in Plant Disease, 95, no. 4 (2011):491-491,
https://doi.org/10.1094/PDIS-10-10-0731 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2685
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.
EP  - 226
IS  - 2
SP  - 226
VL  - 95
DO  - 10.1094/PDIS-10-10-0704
ER  - 

@article{
author = "Stanković, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Jović, Jelena and Krstić, Branka",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.",
pages = "226-226",
number = "2",
volume = "95",
doi = "10.1094/PDIS-10-10-0704"
}

Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Jović, J.,& Krstić, B.. (2011). First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(2), 226-226.
https://doi.org/10.1094/PDIS-10-10-0704

Stanković I, Bulajić A, Vučurović A, Ristić D, Jović J, Krstić B. First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.. in Plant Disease. 2011;95(2):226-226.
doi:10.1094/PDIS-10-10-0704 .

Stanković, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Jović, Jelena, Krstić, Branka, "First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia." in Plant Disease, 95, no. 2 (2011):226-226,
https://doi.org/10.1094/PDIS-10-10-0704 . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2663
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia
EP  - 1035
IS  - 8
SP  - 1035
VL  - 95
DO  - 10.1094/PDIS-02-11-0147
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Jović, Jelena and Krstić, Branka",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia",
pages = "1035-1035",
number = "8",
volume = "95",
doi = "10.1094/PDIS-02-11-0147"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J.,& Krstić, B.. (2011). First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(8), 1035-1035.
https://doi.org/10.1094/PDIS-02-11-0147

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Jović J, Krstić B. First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia. in Plant Disease. 2011;95(8):1035-1035.
doi:10.1094/PDIS-02-11-0147 .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Jović, Jelena, Krstić, Branka, "First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia" in Plant Disease, 95, no. 8 (2011):1035-1035,
https://doi.org/10.1094/PDIS-02-11-0147 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2614
AB  - In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005-2007 and the most frequent were those of PVY A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.
PB  - Aepress Sro, Bratislava
T2  - Acta Virologica
T1  - Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates
EP  - 347
IS  - 4
SP  - 337
VL  - 55
DO  - 10.4149/av_2011_04_337
ER  - 

@article{
author = "Stanković, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Berenji, Janoš and Krstić, Branka",
year = "2011",
abstract = "In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005-2007 and the most frequent were those of PVY A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.",
publisher = "Aepress Sro, Bratislava",
journal = "Acta Virologica",
title = "Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates",
pages = "347-337",
number = "4",
volume = "55",
doi = "10.4149/av_2011_04_337"
}

Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Berenji, J.,& Krstić, B.. (2011). Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. in Acta Virologica
Aepress Sro, Bratislava., 55(4), 337-347.
https://doi.org/10.4149/av_2011_04_337

Stanković I, Bulajić A, Vučurović A, Ristić D, Milojević K, Berenji J, Krstić B. Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. in Acta Virologica. 2011;55(4):337-347.
doi:10.4149/av_2011_04_337 .

Stanković, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Berenji, Janoš, Krstić, Branka, "Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates" in Acta Virologica, 55, no. 4 (2011):337-347,
https://doi.org/10.4149/av_2011_04_337 . .