TY  - JOUR
AU  - Stupar, P.
AU  - Pavlović, Vladimir
AU  - Nunić, J.
AU  - Cundrić, S.
AU  - Filipić, M.
AU  - Stevanović, M.
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3428
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR). scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). testing cytotoxicity as the quality of being toxic to cells, and DCFH-DA assay (2',7'-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
EP  - 197
IS  - 2
SP  - 191
VL  - 24
DO  - 10.1016/S1773-2247(14)50031-7
ER  - 

@article{
author = "Stupar, P. and Pavlović, Vladimir and Nunić, J. and Cundrić, S. and Filipić, M. and Stevanović, M.",
year = "2014",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR). scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). testing cytotoxicity as the quality of being toxic to cells, and DCFH-DA assay (2',7'-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "197-191",
number = "2",
volume = "24",
doi = "10.1016/S1773-2247(14)50031-7"
}

Stupar, P., Pavlović, V., Nunić, J., Cundrić, S., Filipić, M.,& Stevanović, M.. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology
Elsevier Science Bv, Amsterdam., 24(2), 191-197.
https://doi.org/10.1016/S1773-2247(14)50031-7

Stupar P, Pavlović V, Nunić J, Cundrić S, Filipić M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology. 2014;24(2):191-197.
doi:10.1016/S1773-2247(14)50031-7 .

Stupar, P., Pavlović, Vladimir, Nunić, J., Cundrić, S., Filipić, M., Stevanović, M., "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species" in Journal of Drug Delivery Science and Technology, 24, no. 2 (2014):191-197,
https://doi.org/10.1016/S1773-2247(14)50031-7 . .