Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
Апстракт
A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR). scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). testing cytoto...xicity as the quality of being toxic to cells, and DCFH-DA assay (2',7'-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
Кључне речи:
PCL / Lyophilization / Cryoprotectants / Biocompatibility / CytotoxicityИзвор:
Journal of Drug Delivery Science and Technology, 2014, 24, 2, 191-197Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Молекуларно дизајнирање наночестица контролисаних морфолошких и физичко-хемијских карактеристика и функционалних материјала на њиховој основи (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45004)
DOI: 10.1016/S1773-2247(14)50031-7
ISSN: 1773-2247
WoS: 000339928800011
Scopus: 2-s2.0-84899567719
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Stupar, P. AU - Pavlović, Vladimir AU - Nunić, J. AU - Cundrić, S. AU - Filipić, M. AU - Stevanović, M. PY - 2014 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3428 AB - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR). scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). testing cytotoxicity as the quality of being toxic to cells, and DCFH-DA assay (2',7'-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Drug Delivery Science and Technology T1 - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species EP - 197 IS - 2 SP - 191 VL - 24 DO - 10.1016/S1773-2247(14)50031-7 ER -
@article{ author = "Stupar, P. and Pavlović, Vladimir and Nunić, J. and Cundrić, S. and Filipić, M. and Stevanović, M.", year = "2014", abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR). scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). testing cytotoxicity as the quality of being toxic to cells, and DCFH-DA assay (2',7'-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Drug Delivery Science and Technology", title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species", pages = "197-191", number = "2", volume = "24", doi = "10.1016/S1773-2247(14)50031-7" }
Stupar, P., Pavlović, V., Nunić, J., Cundrić, S., Filipić, M.,& Stevanović, M.. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology Elsevier Science Bv, Amsterdam., 24(2), 191-197. https://doi.org/10.1016/S1773-2247(14)50031-7
Stupar P, Pavlović V, Nunić J, Cundrić S, Filipić M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology. 2014;24(2):191-197. doi:10.1016/S1773-2247(14)50031-7 .
Stupar, P., Pavlović, Vladimir, Nunić, J., Cundrić, S., Filipić, M., Stevanović, M., "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species" in Journal of Drug Delivery Science and Technology, 24, no. 2 (2014):191-197, https://doi.org/10.1016/S1773-2247(14)50031-7 . .