TY  - JOUR
AU  - Stupar, P.
AU  - Pavlović, Vladimir
AU  - Nunić, J.
AU  - Cundrić, S.
AU  - Filipić, M.
AU  - Stevanović, M.
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3428
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR). scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). testing cytotoxicity as the quality of being toxic to cells, and DCFH-DA assay (2',7'-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
EP  - 197
IS  - 2
SP  - 191
VL  - 24
DO  - 10.1016/S1773-2247(14)50031-7
ER  - 

@article{
author = "Stupar, P. and Pavlović, Vladimir and Nunić, J. and Cundrić, S. and Filipić, M. and Stevanović, M.",
year = "2014",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR). scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). testing cytotoxicity as the quality of being toxic to cells, and DCFH-DA assay (2',7'-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "197-191",
number = "2",
volume = "24",
doi = "10.1016/S1773-2247(14)50031-7"
}

Stupar, P., Pavlović, V., Nunić, J., Cundrić, S., Filipić, M.,& Stevanović, M.. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology
Elsevier Science Bv, Amsterdam., 24(2), 191-197.
https://doi.org/10.1016/S1773-2247(14)50031-7

Stupar P, Pavlović V, Nunić J, Cundrić S, Filipić M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology. 2014;24(2):191-197.
doi:10.1016/S1773-2247(14)50031-7 .

Stupar, P., Pavlović, Vladimir, Nunić, J., Cundrić, S., Filipić, M., Stevanović, M., "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species" in Journal of Drug Delivery Science and Technology, 24, no. 2 (2014):191-197,
https://doi.org/10.1016/S1773-2247(14)50031-7 . .

TY  - JOUR
AU  - Stevanović, M.
AU  - Pavlović, Vladimir
AU  - Petković, J.
AU  - Filipić, M.
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2674
AB  - Porous, poly(D, L-lactide-co-glycolide) (PLGA) materials were prepared by physicochemical solvent/non-solvent method with polyvinyl pyrrolidone (PVP) as a stabilizer and with silicone oil, paraffin, hydrogen peroxide or sodium chloride as a porogen. The obtained PLGA particles without porogens are non-agglomerated, uniform and with particle size on the submicron scale. The formation of intracellular reactive oxygen species (ROS) was measured spectrophotometrically using a fluorescent probe, 2,7-dichlorofluorescein diacetate (DCFH-DA) and it is shown that PLGA nanospheres are not inducers of intracellular formation. Porous PLGA scaffolds obtained in the experiment with sodium chloride as porogen and water as solvent of the porogen had apparently uniform pore morphology with spheroidal pore in shape and well controlled three-dimensional interconnected network. PLGA scaffolds are highly porous with similar porosity values. The degradation of PLGA nanoparticles and PLGA porous materials were studied in phosphate buffered saline as a degradation medium. The samples were characterized by Infrared Spectroscopy (IR), X-ray difractometry, Zeta potential measurements, Scanning Electron Microscopy (SEM) and Ultraviolet Spectroscopy (UV).
PB  - Budapest Univ Technol & Econ, Budapest
T2  - Express Polymer Letters
T1  - ROS-inducing potential, influence of different porogens and in vitro degradation of poly (D,L-lactide-co-glycolide)-based material
EP  - 1008
IS  - 11
SP  - 996
VL  - 5
DO  - 10.3144/expresspolymlett.2011.97
ER  - 

@article{
author = "Stevanović, M. and Pavlović, Vladimir and Petković, J. and Filipić, M. and Uskoković, Dragan",
year = "2011",
abstract = "Porous, poly(D, L-lactide-co-glycolide) (PLGA) materials were prepared by physicochemical solvent/non-solvent method with polyvinyl pyrrolidone (PVP) as a stabilizer and with silicone oil, paraffin, hydrogen peroxide or sodium chloride as a porogen. The obtained PLGA particles without porogens are non-agglomerated, uniform and with particle size on the submicron scale. The formation of intracellular reactive oxygen species (ROS) was measured spectrophotometrically using a fluorescent probe, 2,7-dichlorofluorescein diacetate (DCFH-DA) and it is shown that PLGA nanospheres are not inducers of intracellular formation. Porous PLGA scaffolds obtained in the experiment with sodium chloride as porogen and water as solvent of the porogen had apparently uniform pore morphology with spheroidal pore in shape and well controlled three-dimensional interconnected network. PLGA scaffolds are highly porous with similar porosity values. The degradation of PLGA nanoparticles and PLGA porous materials were studied in phosphate buffered saline as a degradation medium. The samples were characterized by Infrared Spectroscopy (IR), X-ray difractometry, Zeta potential measurements, Scanning Electron Microscopy (SEM) and Ultraviolet Spectroscopy (UV).",
publisher = "Budapest Univ Technol & Econ, Budapest",
journal = "Express Polymer Letters",
title = "ROS-inducing potential, influence of different porogens and in vitro degradation of poly (D,L-lactide-co-glycolide)-based material",
pages = "1008-996",
number = "11",
volume = "5",
doi = "10.3144/expresspolymlett.2011.97"
}

Stevanović, M., Pavlović, V., Petković, J., Filipić, M.,& Uskoković, D.. (2011). ROS-inducing potential, influence of different porogens and in vitro degradation of poly (D,L-lactide-co-glycolide)-based material. in Express Polymer Letters
Budapest Univ Technol & Econ, Budapest., 5(11), 996-1008.
https://doi.org/10.3144/expresspolymlett.2011.97

Stevanović M, Pavlović V, Petković J, Filipić M, Uskoković D. ROS-inducing potential, influence of different porogens and in vitro degradation of poly (D,L-lactide-co-glycolide)-based material. in Express Polymer Letters. 2011;5(11):996-1008.
doi:10.3144/expresspolymlett.2011.97 .

Stevanović, M., Pavlović, Vladimir, Petković, J., Filipić, M., Uskoković, Dragan, "ROS-inducing potential, influence of different porogens and in vitro degradation of poly (D,L-lactide-co-glycolide)-based material" in Express Polymer Letters, 5, no. 11 (2011):996-1008,
https://doi.org/10.3144/expresspolymlett.2011.97 . .