TY  - JOUR
AU  - Núñez, Yolanda
AU  - Radović, Čedomir
AU  - Savić, Radomir
AU  - García-Casco, Juan M.
AU  - Čandek-Potokar, Marjeta
AU  - Benítez, Rita
AU  - Radojković, Dragan
AU  - Lukić, Miloš
AU  - Gogić, Marija
AU  - Muñoz, María
AU  - Fontanesi, Luca
AU  - Óvilo, Cristina
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5827
AB  - This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.
PB  - MDPI AG
T2  - Animals
T1  - Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs
EP  - 24
IS  - 3
SP  - 1
VL  - 11
DO  - 10.3390/ani11030844
ER  - 

@article{
author = "Núñez, Yolanda and Radović, Čedomir and Savić, Radomir and García-Casco, Juan M. and Čandek-Potokar, Marjeta and Benítez, Rita and Radojković, Dragan and Lukić, Miloš and Gogić, Marija and Muñoz, María and Fontanesi, Luca and Óvilo, Cristina",
year = "2021",
abstract = "This work was aimed at evaluating loin transcriptome and metabolic pathway differences between the two main Serbian local pig breeds with divergent characteristics regarding muscle growth and fatness, as well as exploring nutrigenomic effects of tannin supplementation in Mangalitsa (MA) pigs. The study comprised 24 Mangalitsa and 10 Moravka (MO) males, which were kept under identical management conditions. Mangalitsa animals were divided in two nutritional groups (n = 12) receiving a standard (control) or tannin–supplemented diet (1.5%; MAT). Moravka pigs were fed the standard mixture. All animals were slaughtered at a similar age; 120 kg of average live weight (LW) and loin tissue was used for RNA‐seq analysis. Results showed 306 differentially expressed genes (DEGs) according to breed, enriched in genes involved in growth, lipid metabolism, protein metabolism and muscle development, such as PDK4, FABP4, MYOD1 and STAT3, as well as a relevant number of genes involved in mitochondrial respiratory activity (MT‐NDs, NDUFAs among others). Oxidative phosphorylation was the most significantly affected pathway, activated in Mangalitsa muscle, revealing the basis of a different muscle metabolism. Also, many other relevant pathways were affected by breed and involved in oxidative stress response, fat accumulation and development of skeletal muscle. Results also allowed the identification of potential regulators and causal networks such as those controlled by FLCN, PPARGC1A or PRKAB1 with relevant regulatory roles on DEGs involved in mitochondrial and lipid metabolism, or IL3 and TRAF2 potentially controlling DEGs involved in muscle development. The Tannin effect on transcriptome was small, with only 23 DEGs, but included interesting ones involved in lipid deposition such as PPARGC1B. The results indicate a significant effect of the breed on muscle tissue gene expression, affecting relevant biological pathways and allowing the identification of strong regulatory candidate genes to underlie the gene expression and phenotypic differences between the compared groups.",
publisher = "MDPI AG",
journal = "Animals",
title = "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs",
pages = "24-1",
number = "3",
volume = "11",
doi = "10.3390/ani11030844"
}

Núñez, Y., Radović, Č., Savić, R., García-Casco, J. M., Čandek-Potokar, M., Benítez, R., Radojković, D., Lukić, M., Gogić, M., Muñoz, M., Fontanesi, L.,& Óvilo, C.. (2021). Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals
MDPI AG., 11(3), 1-24.
https://doi.org/10.3390/ani11030844

Núñez Y, Radović Č, Savić R, García-Casco JM, Čandek-Potokar M, Benítez R, Radojković D, Lukić M, Gogić M, Muñoz M, Fontanesi L, Óvilo C. Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs. in Animals. 2021;11(3):1-24.
doi:10.3390/ani11030844 .

Núñez, Yolanda, Radović, Čedomir, Savić, Radomir, García-Casco, Juan M., Čandek-Potokar, Marjeta, Benítez, Rita, Radojković, Dragan, Lukić, Miloš, Gogić, Marija, Muñoz, María, Fontanesi, Luca, Óvilo, Cristina, "Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs" in Animals, 11, no. 3 (2021):1-24,
https://doi.org/10.3390/ani11030844 . .

TY  - JOUR
AU  - Bovo, Samuele
AU  - Schiavo, Giuseppina
AU  - Ribani, Anisa
AU  - Utzeri, Valerio J.
AU  - Taurisano, Valeria
AU  - Ballan, Mohamad
AU  - Muñoz, Maria
AU  - Alves, Estefania
AU  - Araujo, Jose P.
AU  - Bozzi, Riccardo
AU  - Charneca, Rui
AU  - Di Palma, Federica
AU  - Djurkin Kušec, Ivona
AU  - Etherington, Graham
AU  - Fernandez, Ana I.
AU  - García, Fabián
AU  - García‑Casco, Juan
AU  - Karolyi, Danijel
AU  - Gallo, Maurizio
AU  - Martins, José Manuel
AU  - Mercat, Marie‑José
AU  - Núñez, Yolanda
AU  - Quintanilla, Raquel
AU  - Radović, Čedomir
AU  - Razmaite, Violeta
AU  - Riquet, Juliette
AU  - Savić, Radomir
AU  - Škrlep, Martin
AU  - Usai, Graziano
AU  - Zimmer, Christoph
AU  - Ovilo, Cristina
AU  - Fontanesi, Luca
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5807
AB  - Coronaviruses silently circulate in human and animal populations, causing mild to severe diseases. Therefore, livestock are important components of a “One Health” perspective aimed to control these viral infections. However, at present there is no example that considers pig genetic resources in this context. In this study, we investigated the variability of four genes (ACE2, ANPEP and DPP4 encoding for host receptors of the viral spike proteins and TMPRSS2 encoding for a host proteinase) in 23 European (19 autochthonous and three commercial breeds and one wild boar population) and two Asian Sus scrofa populations. A total of 2229 variants were identified in the four candidate genes: 26% of them were not previously described; 29 variants affected the protein sequence and might potentially interact with the infection mechanisms. The results coming from this work are a first step towards a “One Health” perspective that should consider conservation programs of pig genetic resources with twofold objectives: (i) genetic resources could be reservoirs of host gene variability useful to design selection programs to increase resistance to coronaviruses; (ii) the described variability in genes involved in coronavirus infections across many different pig populations might be part of a risk assessment including pig genetic resources
PB  - Nature Research
T2  - Scientific Reports
T1  - Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources
IS  - 1
SP  - 3359
VL  - 11
DO  - 10.1038/s41598-021-82956-0
ER  - 

@article{
author = "Bovo, Samuele and Schiavo, Giuseppina and Ribani, Anisa and Utzeri, Valerio J. and Taurisano, Valeria and Ballan, Mohamad and Muñoz, Maria and Alves, Estefania and Araujo, Jose P. and Bozzi, Riccardo and Charneca, Rui and Di Palma, Federica and Djurkin Kušec, Ivona and Etherington, Graham and Fernandez, Ana I. and García, Fabián and García‑Casco, Juan and Karolyi, Danijel and Gallo, Maurizio and Martins, José Manuel and Mercat, Marie‑José and Núñez, Yolanda and Quintanilla, Raquel and Radović, Čedomir and Razmaite, Violeta and Riquet, Juliette and Savić, Radomir and Škrlep, Martin and Usai, Graziano and Zimmer, Christoph and Ovilo, Cristina and Fontanesi, Luca",
year = "2021",
abstract = "Coronaviruses silently circulate in human and animal populations, causing mild to severe diseases. Therefore, livestock are important components of a “One Health” perspective aimed to control these viral infections. However, at present there is no example that considers pig genetic resources in this context. In this study, we investigated the variability of four genes (ACE2, ANPEP and DPP4 encoding for host receptors of the viral spike proteins and TMPRSS2 encoding for a host proteinase) in 23 European (19 autochthonous and three commercial breeds and one wild boar population) and two Asian Sus scrofa populations. A total of 2229 variants were identified in the four candidate genes: 26% of them were not previously described; 29 variants affected the protein sequence and might potentially interact with the infection mechanisms. The results coming from this work are a first step towards a “One Health” perspective that should consider conservation programs of pig genetic resources with twofold objectives: (i) genetic resources could be reservoirs of host gene variability useful to design selection programs to increase resistance to coronaviruses; (ii) the described variability in genes involved in coronavirus infections across many different pig populations might be part of a risk assessment including pig genetic resources",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources",
number = "1",
pages = "3359",
volume = "11",
doi = "10.1038/s41598-021-82956-0"
}

Bovo, S., Schiavo, G., Ribani, A., Utzeri, V. J., Taurisano, V., Ballan, M., Muñoz, M., Alves, E., Araujo, J. P., Bozzi, R., Charneca, R., Di Palma, F., Djurkin Kušec, I., Etherington, G., Fernandez, A. I., García, F., García‑Casco, J., Karolyi, D., Gallo, M., Martins, J. M., Mercat, M., Núñez, Y., Quintanilla, R., Radović, Č., Razmaite, V., Riquet, J., Savić, R., Škrlep, M., Usai, G., Zimmer, C., Ovilo, C.,& Fontanesi, L.. (2021). Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources. in Scientific Reports
Nature Research., 11(1), 3359.
https://doi.org/10.1038/s41598-021-82956-0

Bovo S, Schiavo G, Ribani A, Utzeri VJ, Taurisano V, Ballan M, Muñoz M, Alves E, Araujo JP, Bozzi R, Charneca R, Di Palma F, Djurkin Kušec I, Etherington G, Fernandez AI, García F, García‑Casco J, Karolyi D, Gallo M, Martins JM, Mercat M, Núñez Y, Quintanilla R, Radović Č, Razmaite V, Riquet J, Savić R, Škrlep M, Usai G, Zimmer C, Ovilo C, Fontanesi L. Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources. in Scientific Reports. 2021;11(1):3359.
doi:10.1038/s41598-021-82956-0 .

Bovo, Samuele, Schiavo, Giuseppina, Ribani, Anisa, Utzeri, Valerio J., Taurisano, Valeria, Ballan, Mohamad, Muñoz, Maria, Alves, Estefania, Araujo, Jose P., Bozzi, Riccardo, Charneca, Rui, Di Palma, Federica, Djurkin Kušec, Ivona, Etherington, Graham, Fernandez, Ana I., García, Fabián, García‑Casco, Juan, Karolyi, Danijel, Gallo, Maurizio, Martins, José Manuel, Mercat, Marie‑José, Núñez, Yolanda, Quintanilla, Raquel, Radović, Čedomir, Razmaite, Violeta, Riquet, Juliette, Savić, Radomir, Škrlep, Martin, Usai, Graziano, Zimmer, Christoph, Ovilo, Cristina, Fontanesi, Luca, "Describing variability in pig genes involved in coronavirus infections for a One Health perspective in conservation of animal genetic resources" in Scientific Reports, 11, no. 1 (2021):3359,
https://doi.org/10.1038/s41598-021-82956-0 . .