TY  - JOUR
AU  - Broćić, Zoran
AU  - Milinković, Mirko
AU  - Momčilović, Ivana
AU  - Poštić, Dobrivoj
AU  - Oljača, Jasmina
AU  - Veljković, Biljana
AU  - Milošević, Drago
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5242
AB  - The effects of variety and origin of planting material were examined in the aeroponic growing system. The potato cultivars 'Cleopatra', 'Kennebec' and 'Agria' were used for the experimental purposes, originating both in vitro and from minitubers of the previous season's crops. Plants originating from minitubers were found to be simpler and cheaper to prepare for the aeroponic system of cultivation compared to plants obtained in vitro. The first harvest of the 'Cleopatra' minitubers began 43 days after planting in aeroponics, whereas the harvest of the 'Kennebec' and 'Agria' minitubers occurred 20 days later. The 'Cleopatra' cultivar yielded an average of 14.37 minitubers, followed by the 'Kennebec' cultivar and "Agria" cultivar with 16.91 and 19.43 minitubers on average, respectively. In the 'Cleopatra' and 'Kennebec' cultivars, the plant of the in vitro origin had a significantly larger number of tubers per plant than the plants originating from previous vegetation minitubers. In the 'Agria' cultivar, the in vitro created plants had a larger number of minitubers. The average mass of minitubers of all the cultivars under consideration was significantly higher in the plants originating from minitubers. A successive harvest of minitubers allows them to reach the desired size with dominant minitubers weighing over 8 g, which are considered large.
AB  - Aeroponski sistem za proizvodnju predosnovnog sadnog materija krompira (bezvirusne mini krtole) počeo je da se primenjuje početkom 21. veka. Mini krtole su proizvedene ex vitro od aklimatizovanih biljaka dobijenih in vitro ili od umnoženih mikro krtola. Njihova veličinu je manja od konvencionalnih krtola semenskog krompira, ali je veća od in vitro krtola proizvedenih pod aseptičnim uslovima na veštačkim medijima. Veličina mini krtola se obično kreće od 5-25 mm. U aeroponik sistemu gajenja, ispitivan je uticaj sorte i porekla sadnog materijala. Kao materijal u istraživanjima korišćene su tri sorte krompira Cleopatra, Kennebec i Agria sa dva porekla: in vitro i od mini krtola iz predhodne generacije. Biljke koje potiču iz mini krtola su jednostavnije i jeftinije za pripremu biljaka pogodnih za aeroponski sistem gajenja, u odnosu na biljke dobijene in vitro. Prvo branje mini krtola kod sorte Cleopatra počelo je 43 dana nakon sadnje u aeroponiku, dok je kod sorti Kennebec i Agria bilo 20 dana kasnije. Sorta Cleopatra formirala je u proseku 14,37 mini krtola, sorta Kennebec 16,91 i Agria 19,43 mini krtole. Kod sorti Cleopatra i Kennebec utvrđen je značajno veći broj krtola po biljci poreklom in vitro, u odnosu na broj krtola po biljci poreklom iz mini krtola iz predhodne vegetacije. Kod sorte Agria zabeležen je veći broj mini krtola poreklom iz sistema in vitro. Prosečna masa mini krtola bila je značajno veća kod biljaka poreklom iz mini krtola, u odnosu na biljke zasnovane iz mini krtola in vitro. Sukcesivno branje mini krtola omogućava da one dostignu željenu veličinu mase preko 8 g.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Effect of the variety and origin of plants on the production of virus-free potato minitubers in the aeroponic growing system
T1  - Uticaj sorte i porekla biljaka na proizvodnju bezvirusnih mini krtola krompira u aeroponik sistemu gajenja
EP  - 149
IS  - 3
SP  - 147
VL  - 23
DO  - 10.5937/JPEA1903147B
ER  - 

@article{
author = "Broćić, Zoran and Milinković, Mirko and Momčilović, Ivana and Poštić, Dobrivoj and Oljača, Jasmina and Veljković, Biljana and Milošević, Drago",
year = "2019",
abstract = "The effects of variety and origin of planting material were examined in the aeroponic growing system. The potato cultivars 'Cleopatra', 'Kennebec' and 'Agria' were used for the experimental purposes, originating both in vitro and from minitubers of the previous season's crops. Plants originating from minitubers were found to be simpler and cheaper to prepare for the aeroponic system of cultivation compared to plants obtained in vitro. The first harvest of the 'Cleopatra' minitubers began 43 days after planting in aeroponics, whereas the harvest of the 'Kennebec' and 'Agria' minitubers occurred 20 days later. The 'Cleopatra' cultivar yielded an average of 14.37 minitubers, followed by the 'Kennebec' cultivar and "Agria" cultivar with 16.91 and 19.43 minitubers on average, respectively. In the 'Cleopatra' and 'Kennebec' cultivars, the plant of the in vitro origin had a significantly larger number of tubers per plant than the plants originating from previous vegetation minitubers. In the 'Agria' cultivar, the in vitro created plants had a larger number of minitubers. The average mass of minitubers of all the cultivars under consideration was significantly higher in the plants originating from minitubers. A successive harvest of minitubers allows them to reach the desired size with dominant minitubers weighing over 8 g, which are considered large., Aeroponski sistem za proizvodnju predosnovnog sadnog materija krompira (bezvirusne mini krtole) počeo je da se primenjuje početkom 21. veka. Mini krtole su proizvedene ex vitro od aklimatizovanih biljaka dobijenih in vitro ili od umnoženih mikro krtola. Njihova veličinu je manja od konvencionalnih krtola semenskog krompira, ali je veća od in vitro krtola proizvedenih pod aseptičnim uslovima na veštačkim medijima. Veličina mini krtola se obično kreće od 5-25 mm. U aeroponik sistemu gajenja, ispitivan je uticaj sorte i porekla sadnog materijala. Kao materijal u istraživanjima korišćene su tri sorte krompira Cleopatra, Kennebec i Agria sa dva porekla: in vitro i od mini krtola iz predhodne generacije. Biljke koje potiču iz mini krtola su jednostavnije i jeftinije za pripremu biljaka pogodnih za aeroponski sistem gajenja, u odnosu na biljke dobijene in vitro. Prvo branje mini krtola kod sorte Cleopatra počelo je 43 dana nakon sadnje u aeroponiku, dok je kod sorti Kennebec i Agria bilo 20 dana kasnije. Sorta Cleopatra formirala je u proseku 14,37 mini krtola, sorta Kennebec 16,91 i Agria 19,43 mini krtole. Kod sorti Cleopatra i Kennebec utvrđen je značajno veći broj krtola po biljci poreklom in vitro, u odnosu na broj krtola po biljci poreklom iz mini krtola iz predhodne vegetacije. Kod sorte Agria zabeležen je veći broj mini krtola poreklom iz sistema in vitro. Prosečna masa mini krtola bila je značajno veća kod biljaka poreklom iz mini krtola, u odnosu na biljke zasnovane iz mini krtola in vitro. Sukcesivno branje mini krtola omogućava da one dostignu željenu veličinu mase preko 8 g.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Effect of the variety and origin of plants on the production of virus-free potato minitubers in the aeroponic growing system, Uticaj sorte i porekla biljaka na proizvodnju bezvirusnih mini krtola krompira u aeroponik sistemu gajenja",
pages = "149-147",
number = "3",
volume = "23",
doi = "10.5937/JPEA1903147B"
}

Broćić, Z., Milinković, M., Momčilović, I., Poštić, D., Oljača, J., Veljković, B.,& Milošević, D.. (2019). Effect of the variety and origin of plants on the production of virus-free potato minitubers in the aeroponic growing system. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 23(3), 147-149.
https://doi.org/10.5937/JPEA1903147B

Broćić Z, Milinković M, Momčilović I, Poštić D, Oljača J, Veljković B, Milošević D. Effect of the variety and origin of plants on the production of virus-free potato minitubers in the aeroponic growing system. in Journal on Processing and Energy in Agriculture. 2019;23(3):147-149.
doi:10.5937/JPEA1903147B .

Broćić, Zoran, Milinković, Mirko, Momčilović, Ivana, Poštić, Dobrivoj, Oljača, Jasmina, Veljković, Biljana, Milošević, Drago, "Effect of the variety and origin of plants on the production of virus-free potato minitubers in the aeroponic growing system" in Journal on Processing and Energy in Agriculture, 23, no. 3 (2019):147-149,
https://doi.org/10.5937/JPEA1903147B . .

TY  - CONF
AU  - Broćić, Zoran
AU  - Milinković, Mirko
AU  - Momčilović, Ivana
AU  - Oljača, Jasmina
AU  - Veljković, Biljana
AU  - Milošević, Drago
AU  - Postić, Dobrivoj
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5656
AB  - In the laboratory for micropropagation in Potato Research Center in Gucha, in vitro virus-free plants of following potato varieties were produced: Desiree, Kennebec, Agria, Cleopatra and Sinora. Acclimatized and rooted plants were transplanted in previously prepared aeroponik module. During the duration of the experiment, the mini-tubers were successively harvested four times and their mass and number were recorded. The first harvest of mini tubers was out after 40-45 days after transplanting in aeropnik system. The highest average number of mini tubers was of the Desiree variety (15.55), while the lowest number was harvested by varieties Sinora and Cleopatra (10.66 and 10.52). The highest average mass of one mini-tuber per plant was obtained in the Agria variety (8.97 g), followed by the Kennebec variety (7.61 g) and the Kleopatra variety (6.36 g). The smallest mini-tubers were measured in the Desiree and Sinora varieties (5.32 g and 4.83 g). In the aeroponic system, 4.08 times more mini-tubers were obtained compared to the substrate. Aeroponik system offers the potential to improve the production of potato mini tubers.
AB  - U laboratoriji za mikropropagaciju  Centru za krompir u Guči proizvedene su bezvirusne biljke in vitro sorti krompira: Desiree, Kennebec, Agria, Cleopatra i Sinora. Aklimatizovane i ožiljene biljke presađene su u pripremljene aeroponik module. Tokom trajanja ogleda mini krtole su sukcesivno ubirane u četiri termina i merena je njihova masa i broj. Prvo branje mini krtola je obavljeno posle 40-45 dana po presadjivanju u aeropnik sistem. Najveći prosečan broj mini krtola imala je sorta Desiree (15,55), dok su najmanji broj dale sorte  Sinora i Cleopatra (10,66 i 10,52). Najveća prosečna masa jedne mini krtole po biljci ustanovljena je kod sorte Agria (8,97 g), zatim kod sorte Kennebec (7,61 g), odnosno sorte Cleopatra (6,36 g). Najsitnije mini krtole izmerene su kod kod sorti Desiree i Sinora (5,32 g i 4,83 g). U aeroponik sistemu dobijen je 4,08 puta veći broj mini krtola  u odnosu na supstrat. Aeroponik sistem nudi potencijalnu mogućnost za poboljšanje proizvodnje dobijanja mini krtola krompira.
C3  - 23. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 09.-10. 03. 2018.
T1  - Aeroponics, New Technology for Production of Potato Mini Tubers in Guca
T1  - Aeroponika, nova tehnologija za proizvodnju mini krtola krompira u Guči
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5656
ER  - 

@conference{
author = "Broćić, Zoran and Milinković, Mirko and Momčilović, Ivana and Oljača, Jasmina and Veljković, Biljana and Milošević, Drago and Postić, Dobrivoj",
year = "2018",
abstract = "In the laboratory for micropropagation in Potato Research Center in Gucha, in vitro virus-free plants of following potato varieties were produced: Desiree, Kennebec, Agria, Cleopatra and Sinora. Acclimatized and rooted plants were transplanted in previously prepared aeroponik module. During the duration of the experiment, the mini-tubers were successively harvested four times and their mass and number were recorded. The first harvest of mini tubers was out after 40-45 days after transplanting in aeropnik system. The highest average number of mini tubers was of the Desiree variety (15.55), while the lowest number was harvested by varieties Sinora and Cleopatra (10.66 and 10.52). The highest average mass of one mini-tuber per plant was obtained in the Agria variety (8.97 g), followed by the Kennebec variety (7.61 g) and the Kleopatra variety (6.36 g). The smallest mini-tubers were measured in the Desiree and Sinora varieties (5.32 g and 4.83 g). In the aeroponic system, 4.08 times more mini-tubers were obtained compared to the substrate. Aeroponik system offers the potential to improve the production of potato mini tubers., U laboratoriji za mikropropagaciju  Centru za krompir u Guči proizvedene su bezvirusne biljke in vitro sorti krompira: Desiree, Kennebec, Agria, Cleopatra i Sinora. Aklimatizovane i ožiljene biljke presađene su u pripremljene aeroponik module. Tokom trajanja ogleda mini krtole su sukcesivno ubirane u četiri termina i merena je njihova masa i broj. Prvo branje mini krtola je obavljeno posle 40-45 dana po presadjivanju u aeropnik sistem. Najveći prosečan broj mini krtola imala je sorta Desiree (15,55), dok su najmanji broj dale sorte  Sinora i Cleopatra (10,66 i 10,52). Najveća prosečna masa jedne mini krtole po biljci ustanovljena je kod sorte Agria (8,97 g), zatim kod sorte Kennebec (7,61 g), odnosno sorte Cleopatra (6,36 g). Najsitnije mini krtole izmerene su kod kod sorti Desiree i Sinora (5,32 g i 4,83 g). U aeroponik sistemu dobijen je 4,08 puta veći broj mini krtola  u odnosu na supstrat. Aeroponik sistem nudi potencijalnu mogućnost za poboljšanje proizvodnje dobijanja mini krtola krompira.",
journal = "23. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 09.-10. 03. 2018.",
title = "Aeroponics, New Technology for Production of Potato Mini Tubers in Guca, Aeroponika, nova tehnologija za proizvodnju mini krtola krompira u Guči",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5656"
}

Broćić, Z., Milinković, M., Momčilović, I., Oljača, J., Veljković, B., Milošević, D.,& Postić, D.. (2018). Aeroponics, New Technology for Production of Potato Mini Tubers in Guca. in 23. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 09.-10. 03. 2018..
https://hdl.handle.net/21.15107/rcub_agrospace_5656

Broćić Z, Milinković M, Momčilović I, Oljača J, Veljković B, Milošević D, Postić D. Aeroponics, New Technology for Production of Potato Mini Tubers in Guca. in 23. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 09.-10. 03. 2018.. 2018;.
https://hdl.handle.net/21.15107/rcub_agrospace_5656 .

Broćić, Zoran, Milinković, Mirko, Momčilović, Ivana, Oljača, Jasmina, Veljković, Biljana, Milošević, Drago, Postić, Dobrivoj, "Aeroponics, New Technology for Production of Potato Mini Tubers in Guca" in 23. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 09.-10. 03. 2018. (2018),
https://hdl.handle.net/21.15107/rcub_agrospace_5656 .

TY  - JOUR
AU  - Broćić, Zoran
AU  - Milinković, Mirko
AU  - Momčilović, Ivana
AU  - Poštić, Dobrivoj
AU  - Oljača, Jasmina
AU  - Veljković, Biljana
AU  - Milošević, Drago
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4908
AB  - At the micropropagation laboratory of the Potato Research Centre in Gucha, the following five virus-free in vitro potato cultivars are produced: 'Desiree', 'Kennebec', 'Agria', 'Cleopatra' and 'Sinora'. Acclimatized and rooted plants were transplanted and grown in the three production systems: 1) the aeroponic system, 2) the substrate system, and 3) a combination of the substrate and aeroponic systems. The results obtained indicate that the cultivation system exerts a significant effect on the number and the total yield of potato mini-tubers. In the aeroponic production system, 4.08 times as many mini-tubers were produced compared to the number of mini-tubers produced in the substrate system, whereas 1.29 times as many mini-tubers were produced compared to the number of mini-tubers produced in the combined production system (substrate + aeroponics). The aeroponic mini-tuber production system offers an opportunity to improve the production of seed potatoes in Serbia..
AB  - Proizvodnja bezvirusnog semenskog krompira danas se odvija preko metode poznate kao kultura tkiva (mikropropagacija in vitro), čija je suština da se za relativno kratko vreme, i u kontrolisanim uslovima, dobije veliki broj bezvirusnih mini ili mikro krtola. U laboratoriji za mikropropagaciju Centru za krompir u Guči proizvedene su bezvirusne biljke in vitro pet sorti krompira: Desiree, Kennebec, Agria, Cleopatra i Sinora. Aklimatizovane i ožiljene biljke presađene su i gajene u tri sistema proizvodnje: 1) aeroponik, 2) u supstratu i 3) kombinaciji dva sistema gajenja (supstrat+aeroponik). Cilj rada je bio da se izvrši poređenje aeroponik sistema gajenja sa druga dva sistema proizvodnje mini krtola. Mini krtole u aeroponik sistemu posle 40-45 dana gajenja sukcesivno su ubirane u četiri roka berbe u intervalima od 10-15 dana. U ostala dva sistemima gajenja mini krtole su vađene na kraju vegetacionog perioda. Analiziran je broj mini krtola po m2, prosečna masa krtola i ukupan prinos. Najveći prosečan broj mini krtola utvrđen je kod sorte Desiree, dok je najmanji broj mini krtola zabeležen kod sorte Sinora i Cleopatra. Kod sorte Agria konstatovan je najveći prosečnan prinos mini krtola, a zatim kod sorte Kennebec. Dobijeni rezultati ukazuju da sistem gajenja značajno utiče broj mini krtola i ukupan prinos. Kod aeroponik sistema proizvodnje postignut je 4,08 puta veći broj mini krtola, u odnosu na broj mini krtola dobijen u supstratu, odnosno 1,29 puta veći broj mini krtola, u odnosu na kobinovani način proizvodnje (supstrata+aeroponik). Aeroponik sistem proizvodnje mini krtola nudi potencijalnu mogućnost za poboljšanje proizvodnje semenskog krompira u Srbiji.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Production of potato mini-tubers in the aeroponic growing system
T1  - Proizvodnja mini krtola krompira u aeroponik sistemu gajenja
EP  - 52
IS  - 1
SP  - 49
VL  - 22
DO  - 10.5937/JPEA1801049B
ER  - 

@article{
author = "Broćić, Zoran and Milinković, Mirko and Momčilović, Ivana and Poštić, Dobrivoj and Oljača, Jasmina and Veljković, Biljana and Milošević, Drago",
year = "2018",
abstract = "At the micropropagation laboratory of the Potato Research Centre in Gucha, the following five virus-free in vitro potato cultivars are produced: 'Desiree', 'Kennebec', 'Agria', 'Cleopatra' and 'Sinora'. Acclimatized and rooted plants were transplanted and grown in the three production systems: 1) the aeroponic system, 2) the substrate system, and 3) a combination of the substrate and aeroponic systems. The results obtained indicate that the cultivation system exerts a significant effect on the number and the total yield of potato mini-tubers. In the aeroponic production system, 4.08 times as many mini-tubers were produced compared to the number of mini-tubers produced in the substrate system, whereas 1.29 times as many mini-tubers were produced compared to the number of mini-tubers produced in the combined production system (substrate + aeroponics). The aeroponic mini-tuber production system offers an opportunity to improve the production of seed potatoes in Serbia.., Proizvodnja bezvirusnog semenskog krompira danas se odvija preko metode poznate kao kultura tkiva (mikropropagacija in vitro), čija je suština da se za relativno kratko vreme, i u kontrolisanim uslovima, dobije veliki broj bezvirusnih mini ili mikro krtola. U laboratoriji za mikropropagaciju Centru za krompir u Guči proizvedene su bezvirusne biljke in vitro pet sorti krompira: Desiree, Kennebec, Agria, Cleopatra i Sinora. Aklimatizovane i ožiljene biljke presađene su i gajene u tri sistema proizvodnje: 1) aeroponik, 2) u supstratu i 3) kombinaciji dva sistema gajenja (supstrat+aeroponik). Cilj rada je bio da se izvrši poređenje aeroponik sistema gajenja sa druga dva sistema proizvodnje mini krtola. Mini krtole u aeroponik sistemu posle 40-45 dana gajenja sukcesivno su ubirane u četiri roka berbe u intervalima od 10-15 dana. U ostala dva sistemima gajenja mini krtole su vađene na kraju vegetacionog perioda. Analiziran je broj mini krtola po m2, prosečna masa krtola i ukupan prinos. Najveći prosečan broj mini krtola utvrđen je kod sorte Desiree, dok je najmanji broj mini krtola zabeležen kod sorte Sinora i Cleopatra. Kod sorte Agria konstatovan je najveći prosečnan prinos mini krtola, a zatim kod sorte Kennebec. Dobijeni rezultati ukazuju da sistem gajenja značajno utiče broj mini krtola i ukupan prinos. Kod aeroponik sistema proizvodnje postignut je 4,08 puta veći broj mini krtola, u odnosu na broj mini krtola dobijen u supstratu, odnosno 1,29 puta veći broj mini krtola, u odnosu na kobinovani način proizvodnje (supstrata+aeroponik). Aeroponik sistem proizvodnje mini krtola nudi potencijalnu mogućnost za poboljšanje proizvodnje semenskog krompira u Srbiji.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Production of potato mini-tubers in the aeroponic growing system, Proizvodnja mini krtola krompira u aeroponik sistemu gajenja",
pages = "52-49",
number = "1",
volume = "22",
doi = "10.5937/JPEA1801049B"
}

Broćić, Z., Milinković, M., Momčilović, I., Poštić, D., Oljača, J., Veljković, B.,& Milošević, D.. (2018). Production of potato mini-tubers in the aeroponic growing system. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 22(1), 49-52.
https://doi.org/10.5937/JPEA1801049B

Broćić Z, Milinković M, Momčilović I, Poštić D, Oljača J, Veljković B, Milošević D. Production of potato mini-tubers in the aeroponic growing system. in Journal on Processing and Energy in Agriculture. 2018;22(1):49-52.
doi:10.5937/JPEA1801049B .

Broćić, Zoran, Milinković, Mirko, Momčilović, Ivana, Poštić, Dobrivoj, Oljača, Jasmina, Veljković, Biljana, Milošević, Drago, "Production of potato mini-tubers in the aeroponic growing system" in Journal on Processing and Energy in Agriculture, 22, no. 1 (2018):49-52,
https://doi.org/10.5937/JPEA1801049B . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Dragićević, Ivana
AU  - Pantelić, D.
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3023
AB  - We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)
EP  - 144
IS  - 1
SP  - 135
VL  - 64
DO  - 10.2298/ABS1201135S
ER  - 

@article{
author = "Savić, Jelena and Dragićević, Ivana and Pantelić, D. and Oljača, Jasmina and Momčilović, Ivana",
year = "2012",
abstract = "We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)",
pages = "144-135",
number = "1",
volume = "64",
doi = "10.2298/ABS1201135S"
}

Savić, J., Dragićević, I., Pantelić, D., Oljača, J.,& Momčilović, I.. (2012). Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(1), 135-144.
https://doi.org/10.2298/ABS1201135S

Savić J, Dragićević I, Pantelić D, Oljača J, Momčilović I. Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences. 2012;64(1):135-144.
doi:10.2298/ABS1201135S .

Savić, Jelena, Dragićević, Ivana, Pantelić, D., Oljača, Jasmina, Momčilović, Ivana, "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)" in Archives of Biological Sciences, 64, no. 1 (2012):135-144,
https://doi.org/10.2298/ABS1201135S . .